TW201247102A - Method for cultivating sugar cane - Google Patents

Abstract

The present invention relates to a method for cultivating sugar cane comprising cutting the stalk of an adult sugar cane plant into pieces, placing the stalk pieces containing at least one node in a humid medium, removing the shoots emerged from the nodes of the stalk or of the stalk pieces, optionally removing the newly emerged shoots, where this step can be repeated one or several times, removing the newly formed shoots when they are 10 to 60 cm long in such a way that they comprise meristematic tissue, planting these shoots in a growth medium, growing seedlings from the shoots at a temperature of at least 15 DEG C, and 10 to 120 days after planting the shoots, planting the seedlings grown from the shoots to the field if the growth medium is not a field, or, in case the growth medium is a field, exposing the seedlings obtained from the shoots to ambient conditions.

Description

201247102 VI. Description of the Invention: [Technical Field] The present invention relates to a method for cultivating sugar cane, which comprises cutting a stalk of a mature sugar cane plant into pieces, and placing a test piece containing at least one stem section in a wet medium. In the middle, the germination sprouts are removed from the strict stalk section or the M-like piece, and the new sprouting sprouts are removed as needed. This step can be repeated one or several times, and the newly formed sprouts of up to 60 cm length are removed to include them. The meristematic tissue is cultivated in the growth medium, and the new shoots are grown at at least the temperature, and 10 to 12 days after the cultivation of the new shoots. If the growth medium is not the field, the seedlings will be grown from the new shoots. When cultivated into the field, or when the growth medium is field, 'the seedlings grown from the new shoots are exposed to the surrounding environment. [Prior Art] Considering the limited nature of fossil fuels, it has been re-recognized that biofuels are important energy sources. Sugar cane is used in plants for the production of biofuels (bioethanol) and is believed to be useful for further use, providing a renewable and clean fuel for the alcohol obtained from the fermentation of such plants. The area of planting and growing in the world is increasing, and the investment in the production of alcohol from the wasteland in the factory is also increasing. ^Seed from the single-pistil, ovary wall (peel) and the seed coat to form a dry single seed fruit or fruit. The seeds are 彡卩m-shaped, yellow-colored and extremely small, about 1 mm long. The disadvantage is that sugar cane seeds only germinate under certain environmental characteristics (eg, constant warm and humid climate conditions). These climatic conditions & methods exist in every place where they are long, and therefore the B-horn buds of the seeds of the sorghum are not always guaranteed. In the case of commercial agriculture, Gan Lian is vegetatively propagated by planting stem segments (or part of sugarcane stalks or grass stalks or young crops) without planting or planting the cultivar 163649.doc 201247102. As mentioned above, the stem of sugar cane, and the stem of the grass plant, contain several knots from which new plants will grow. The traditional method of planting sugar cane involves retaining the crop area as a plant source for replanting because of the inclusion of nodes in the stem. The plants for replanting are harvested and subsequently cut into sections of about 20 to 5 cm so that there are J 2 nodes in each stem segment (seU). The stem is cut to destroy the apex advantage, otherwise it can cause poor sprouting when using full length (uncut) stems. The segments are cut to have at least 2 buds (or at least two segments; each segment typically has buds) to ensure germination, since not every bud will germinate. Existing machines used to cut the ginseng section do not recognize any of the characteristics of the stem, and thus the exact location of the cut point can only be determined at random. After cutting, the stem segments are arranged parallel to each other in the gully of the soil, and the gullies are generally open to the ground surface 'wide and deep (4 〇 to 5 〇 cm wide and 3 〇 to 4 〇) (10) Deep), and then slightly cover the soil. Although this planting technique has been used to date, the overall process is relatively inefficient due to the need to use many sections of 2 to 4 knots to ensure germination. The results require large areas for replanting, and therefore the area that could otherwise be used for crops and alcohol or sugar must be retained for replanting. Therefore, it is necessary to improve the efficiency of Gangan cultivation technology. Recently, from the cultivation method of Syngenta (referred to as Magic (3) (10)), the section of less than 4 cm in length was separated from the stem, so that it was treated with the Syngenta seed product, and then planted on the field. It is said that the method is 7% production: increase by up to 丨5%. However, the area required for '% colonization is still enormous. + 1

The cultivation method is also described in WO 163649.doc 201247102

2009/000398, WO 2009/000399, WO 2009/000400, WO 2009/000401 and WO 2009/000402. JP 08-280244 describes a method for producing sugarcane seedlings which causes buds to germinate from sugarcane side buds, allowing the buds to grow, removing the head of the bud that has grown, thereby allowing the buds to self-cut buds The lateral buds of the body are germinated, and the process steps are repeated one or several times. It is said that this process can produce a large number of sugarcane seedlings. A similar method is described in JP 2003-204716 and JP 2000-135025. Therefore, it is an object of the present invention to provide a method for cultivating sugar cane which requires a significant reduction in the area for reproduction, so that the time for using the propagation area is short, Reproduction is less dependent on the cultivated area and a healthy and resistant plant can be obtained. SUMMARY OF THE INVENTION This object is achieved by a method for cultivating sugar cane, the method comprising: (0 cutting a sugar cane plant of 6 to 18 months old into a lump; (II) at least one fungicidal as needed And/or at least one insecticide and/or at least one wound protection material and/or at least one growth regulator to treat the cut surface of the cane bar obtained in step (1); (III) will be in step (1) or (Π) The four sugarcane pieces obtained in at least one section are placed in a wet medium; (lv) are cut from the top of the meristem in the sprout of the stalk of the cane; (V) as needed Treating the cut surface of the lateral sprout obtained in step 163649.doc 201247102 (iv) with at least one fungicide and/or at least one insecticide and/or at least one growth regulator; (vi) removing the step in the step as needed ( The newly formed sprouts of the cut new teeth obtained in iv) or (v) are located on top of the meristem; (vii) by at least one fungicide and/or at least one insecticide and/or as needed Or at least one growth regulation Treating the cut surface of the side sprout obtained in step (vi); (νΐϋ) repeating step (vi) and optionally step (vii) one or several times as needed; (ix) when from step (iv), (v) , (vi), (vii) or (vni) the newly formed side shoots germinated by the cut sprouts are 10 to 60 cm long, 'cut them to include at least a portion of the meristems; X) treating the sprout obtained in step (ix) with at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one growth regulator, if desired; (xi) Planting the shoots obtained in step (ix) or (X) in a growth medium; Ο 11) using at least one fertilizer and/or at least one fungicide and, as needed, shortly before, during or after planting / or at least one insecticide and / or at least one nematicide and / or at least one growth regulator and / or at least one super adsorbent and / or at least one growth promoting bacteria treatment of sprouts and / or growth medium; 163649. Doc 201247102 (xiii) Make sprouts at least 15 °C Growing seedlings at temperature; (xiv) by at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one growth regulator and/or at least one hair root agent, as desired And/or growth-promoting bacteria to treat seedlings and/or their growth medium during growth; (XV) seedlings obtained from new shoots after 10 to 120 days of planting the shoots in the growth medium, if the growth medium is not the field Planted to the field, wherein the field has been grown by at least one fertilizer and/or at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one prior to or during planting. Conditioning agent and/or at least one super adsorbent and/or growth promoting bacteria treatment, or if the growth medium is fielded, 'the seedlings obtained from the sprout are exposed to the surrounding environment; and (xvi) being planted in the field as needed By at least one fertilizer and/or at least one fungicide and/or at least one insecticide and/or at least one during or after exposure to the surrounding environment Nematocides and / or at least one growth regulating agent and / or at least one super-adsorbent and / or seedlings and / or in the field of promoting bacterial growth and / or at least one polymer preservation process. [Embodiment] For the purposes of the present invention, 'stem' is the main stem or stalk of the grass stalk portion of the grass plant (here: sugar cane plant), that is, the trunk of the plant, specifically, forms 163649.doc 201247102 The main plant axis of buds and sprouts. A "sett" is a segment or segment of a stem segment having one or more segments. '' is the location where new shoots, buds or small shoots are formed in the stems of grasses (here: Ganshen plants). The sprout "#" or "small bud" is the embryo, spore or germ of the grass (here: sugar cane plant). "Sprout" is a new plant that sprouts from sprouts/buds. Young field is a young plant that sprouts/buds from sprouts/buds. As of this month. The young plants from the time of germination/germination to planting to the field (if the growth medium in which the new shoots first grow is not the field) or until the young plants are exposed to the surrounding environment (if the sprouts are directly planted in the field) are called "seedlings". The following description of the preferred embodiments of the method features of the present invention can be applied in combination with each other, in particular, in combination with each other. When the stem of the removed plant is cut into pieces in step (1), the top end advantage is destroyed. The "top advantage" is a physiological phenomenon in plants, whereby the stem of the plant will have an advantage over the lateral stem. The apical bud produces growth hormone auxin, which diffuses downward and inhibits the progression of lateral bud growth, otherwise the lateral buds compete with the tip. Interrupting the auxin circulation and thereby destroying the apical advantage will allow for the development of lower dominant lateral buds. By disrupting the apical dominance, the lateral sprouts are induced to germinate from the nodes on the remaining plants below. In general, each reproductive node produces an average of one sprout. Of course, I63649.doc 201247102 also has non-reproductive festivals and produces more than - Cheong Hong

The reproductive node produces a new shoot.彳 ― big. The removal of the P-tooth in step (w) removes the part of the new cross-section that will induce each segment (or, more accurately, = each residual plant part of the sprout) to produce more than one side sprout...= The average yield peak of each reproductive node - plus ~ month and then the colony and produce less than three new shoots ~ 1 or more than three sprouts, but most sections produce three new shoots. Gentleman's ~ s 'depends on whether the steps are carried out (v〇 and (Viii), the steps (丨) in the sudden, the removal of the sprout will be on average 3 to 6 times more than the step (iv). Therefore 'in this In the method of the invention, 'each of the sugarcane plants used in the step (i) produces an average of 〇 to 12 young 'mothers' _ (five), which is on average twice the number known in the conventional planting. JP ^ JP 08. 280244 ^ jp 2003-204716 and JP 2000-135025 describe the method of the ancient, 1 _. Tingcher, which requires a shorter breeding area, that is, up to the desired age of the mother plant. Because the breeding of sprouts and seedlings can be carried out independently in farmland anywhere, and space and manpower can be applied reasonably. Step (1) is preferably carried out in the case of Ganyan plants up to 8 months! That is, where the growth is strong, and the recommended stalk is still connected with the root), the recommended stalk of the sorghum plant is cut into pieces 'But for practical reasons, it is best to remove the ginseng plant from its growing place first, then Then the stalk is cut into pieces. It is removed from the place where the plant grows to cut it into pieces. The time interval should not be too long to ensure that the new shoots can still be produced. However, the maximum time interval can be affected by the conditions of the removed plants or their storage conditions. For example, 'When stored under humid conditions, the maximum time interval can be extended. However, it is preferred to remove the plant from its growing place, for example, up to 7 days after the removal of the plant from 163649.doc 201247102, preferably up to 5 days, preferably up to 3 days and at most After 2 days, the cane rod is cut into pieces. If the sorghum plant is removed from its growing place and then the t-stalk is cut into pieces, it is better to remove the stalk plant from its growing place before or after (but in Before cutting the cane bar into pieces, the top of the cane is cut so that the remaining samples of the remaining plants still contain about 5 to 15, preferably 8 to 15 and specific ones, 8 to 12 knots. First remove the top of the recommendation' and then remove the plant: the plant is removed from its place of growth. If the topmost section removes one of the tops, this removal also helps to destroy the apical advantage, but for this purpose. Not necessary steps Because it is only necessary to cut the recommendation into pieces to achieve this purpose. For practical reasons (eg, easier to transport and handle "head-to-head"), it is not a preferred embodiment to cut the top." "Excision" means any suitable process for removing the top, such as, for example, by cutting a knife, a mountain knife, an axe, a miscellaneous or any suitable machine, cutting or sawing or by manual interruption or Remove the top. The top of the plant can be removed manually or automatically. The plant or its "head" cane can be removed with its underground part. This process can be carried out by any suitable means, cutting, rooting, excavating or lifting. Manually or automatically removed from the roots. It is then better to remove the (original) underground part of the self-recommended stalk. The subterranean part can be removed from the axe by any suitable means, such as by cutting or removing or using a machine to break or strip it: for example, by hand or by impact. However, it is better to remove the plant just above the surface of the soil or its "toe stalk. It is better to remove the plant / rigorous" to make it contain plants or 163649.doc 201247102 its "to the head" cane toads All sections or at least 80% of the festival. The cane can be removed or stripped by any suitable means, such as by cutting a knife, a mountain knife, an ax, a saw, a chopping or sawing, or by using a machine, such as by hand or by means of Shock. In a preferred embodiment, the plant/stalk is broken or stripped by impact, for example, by hand or, more specifically, by impact. This has the advantage that diseases that may exist in a single plant do not spread contaminated other healthy plants via tools for cutting/cutting, sawing, etc. (eg, knives, rakes, axes, saws, and the like). The cut surface of the cane stick is optionally provided by at least one fungicide and/or or to a wound protection material and/or at least one growth regulator. This treatment not only protects its "open wounds" but also diseases and pests. It also supports physiological effects. Suitable and preferred anti-killing agents, insecticides, wound protection materials and growth regulators are shown below in -Hai and other killings (4), especially those who also have physiological effects, especially = health and / or growth Promoting the action of fungicides (such as arborvitae; 1 gram (Py (four) she. bin)) treatment. Preferably, the formula is used to treat the surface of the plant. In the context of the present invention, the term "the surface obtained by the plant part is obtained." ^二= any opening obtained by any means of specifying the cut:: two by removing the plant part by any suitable means in the steps, for example, by means of a cutter, a mountain knife, " Cut into blocks 'eg, cut, cut or cut. This process can be done by human or t or any suitable machine cut by manual or automated. 163649.doc

S • 12. 201247102 Of course, the cutting method should be such that a block with a complete section is obtained, that is, the cutting surface is substantially parallel or slightly inclined to the circumference formed by the segments, but must never be perpendicular to the circumference. Preferably, the blocks are cut in such a way that the block contains at least one section, for example 1, 2, 3, 4, 5 or 6 sections, preferably J, 2, 3, 4 or 5 sections, preferably , 丨, 2, 3 or 4 sections; even better [, 2 or 3 sections, in particular, 1 or 2 sections, and especially 1 section. Of course, in order to reduce the size of the block containing at least one of the segments, it is possible to cut out the block without any of the segments (and discard). For example, if the blocks contain a single section, a narrow disk having only the same section and similar area can be cut out and used for subsequent steps, and the block previously between the two sections can be discarded. If the block contains 2 knots, it can be cut close to the 2 knots and discard the intermediate block without any knots. If the cutting is performed automatically, the machine used preferably includes a sensor for detecting the position of the node, for example, a 汛 sensor. In step (ii) as desired, the treatment in step (1) is carried out by at least one fungicide and/or to insecticide and/or at least one wound protection material and/or to a growth regulator. The cut surface of the obtained sugarcane block not only protects the "open wound" from diseases and pests but also supports physiological effects. Suitable and preferred fungicides, insecticidal, wound care 4 materials and growth adjustments are shown below. In the case of fungicides, it is preferred to use at least one of the fungicides (such as propionin and especially kekemin) which also have physiological effects, especially plant health and/or long promoting action. A fungicide (specifically, a scorpion-a fungus, especially a cytochrome), and/or at least one growth regulator. 163649.doc -13· 201247102 In the 7 step ((1)), the block obtained in the step (1) or (8) and containing at least one known block is placed in the wet medium. The time interval between cutting from a rigid rod to the placement of the blocks in the wet medium should not be too long to ensure that the shoots still produce sprouts. However, the maximum time interval can be affected by the storage conditions of the block being cut. For example, saving under wet conditions can extend the maximum time interval. ", preferably, shortly after the cut into pieces, for example, after the cane is cut into pieces, up to 7 days, preferably up to 5 days, more preferably up to 3 days and, in particular, up to 2 days, The cutting block is placed in a wet medium. - The sugar cane block intended to be placed in the wet medium preferably contains at least one knot, for example, 1, 2, 3, 4, 5 or 6 knots, preferably 2 , 3, 4 or 5 knots 'better 1, 2, 3 or 4 knots; even better, 2 or 3 knots, 1 or 2 knots in particular, and especially 1 knot. For steps (iii) Medium wet medium is any medium or means that allows the cane to be wet. This purpose can be achieved not only by any medium suitable for preserving and delivering moisture, but also by nutrient solution or by permanent or periodic An irrigation system that wets, soaks, sprays, rinses or wets the cane cubes to maintain the ability of these sugarcane pieces to produce new shoots. Suitable mediums for storing and transporting water are, for example, soil, sand, clay & Volcanic rock, pumice, Geohumus®, unloaded and partially neutralized acrylic/acrylate Copolymer, such as GEFa Produkte from Germany

Stockosorb® from Fabritz GmbH; Polymeric supersorbents, vermiculite, coconut fiber, wet cellulosic materials, hydroponic systems, gas tillage systems and the like. 163649.doc 201247102 Suitable nutrient solutions are described below. They can be used as nutrient membrane technology systems and irrigation systems. The wet medium is preferably selected from the group consisting of clay, sand, vermiculite and coconut fiber, more preferably selected from vermiculite and coconut fiber, and especially coconut fiber. The medium in which the water can be stored and transferred should of course be kept moist or periodically humidified, for example, by irrigation with water or a nutrient solution, sprayed, inserted into water or a nutrient solution, etc. by at least one fungicide and/or at least one The wet medium is treated with an insecticide and/or to/or a wound protection material and/or at least one growth regulator. This treatment not only protects plant blocks from diseases and pests, but also supports physiological effects. Suitable and preferred fungicides, insecticides, wound protection materials and growth regulators are shown below. In the case of fungicides, it is especially preferred to treat them by fungicides which have physiological effects, in particular plant health and/or growth promoting effects (e.g., icyrubin and especially dexamethasone). Preferably, the sugarcane block is placed in the wet by at least one fungicide (specifically, a serotonin fungicide, especially a kekemin), and/or at least one growth regulator. In the medium, the buds are arranged sideways with respect to the axis of gravity, that is, they are neither pointing to the earth nor facing the earth. It should be solemn, in fact, each section generally holds a (original advantage) bud, from which sprouts can be formed (see next step). This bud is generally not obvious but lateral placement of the buds is still possible to ensure better development of the shoots and/or to make it easier to handle in the next step. After the sprout is developed, step (iv) is carried out. Preferably, step (iv) is initiated when the germinated new bud is preferably 10 to 6 cm, more preferably 25 to 3 cm long and contains at least one segment I63649.doc -15-201247102. In step (w), the at least one of the new shoots that are germinated from the section of the vegetable stalk block (more precisely, the buds from the knot) is located at the top of the meristem above the excised block. Does not contain any meristems. (4) They will not survive because they cannot form any roots, and they will be discarded as usual. The meristem is known to those skilled in the art and can be positioned by its proximity to the node. However, the excised blocks contain at least the top of the sprout. "Excision" as used herein refers to any suitable process for removing the top, such as 'for example, by cutting, chopping or miscuting with a cutter, a mountain knife, an ax, a mine or any suitable machine' or by a person The guard interrupts or strips the top. This removal is preferably done manually by, for example, by breaking, stripping, cutting or removing. In step (v) as required, the bud (4) is removed by at least one fungicide and/or at least one insecticide and/or at least __ growth regulator treatment step (Vi) (4) Surface. This treatment not only protects this wound, but also suffers from diseases and pests, and also supports physiological effects. Suitable and preferred fungicidal insecticides, wound protection materials and growth regulators are shown below. Fungicides +, the use of fungicides which also have physiological effects (especially plant health and/or growth promoting effects) (e.g., fentanin and especially baikemin) are particularly preferred. Preferably, it is treated by at least one fungicide (specifically, a muscarinic fungicide, especially a gram of sensitizer), and/or at least one growth regulator. As described above, removing the top of the sprout in step (iv) will induce a new shoot. 163649.doc

S 201247102 Health - This time the amount produced is increased by the amount directly obtained after removing the top of the sugar cane plant. These newly sprouted sprouts can be used for planting (by step (ix) and subsequent steps, see below) or steps (vi) and (vii) can be performed as needed, which means that steps (iv) and (V) are repeated. The description of steps (iv) and (v) applies equally to steps (vi) and (vii). Step (vi) and (vii) as needed - or several times, may be repeated as needed (viii). Steps (vi) and (vii) can be repeated in principle, but the number of repetitions is often limited by the quality of the newly sprouted sprouts, which will decrease with each repetition' and generally no more than five times. Preferably, step (vi) and (vii) as desired may be performed at most once (or in other words: step (viii) is preferably not repeated). Specifically, the step (Vii) and (viii) 'this means that the new shoot obtained after the step (iv) or the b) will directly proceed to the step (ix). In the step (ix), when the newly formed sprouts germinated from the sprouts cut in the step (iv), (v), (vi), (vii) or (viii) are 10 to 60 cm long, Wait and cut. The cut will be such that it contains at least a portion of the meristem. The presence of meristematic tissue allows the cut shoots to produce roots and growth. For this purpose, the sprouts are cut near the location of their germination. Preferably, when the new sprout is 10 to 40 cm long, more preferably 20 to 40 0111 long and 25 to 30 cm long for the specific a, it is cut. "cut" as used herein, means any suitable manufacturing for the removal or separation of side buds such as 'cutting', cutting or sawing by means of a knife, a mountain knife, an ax, a saw or any suitable machine, or By manually interrupting or stripping the new 163649.doc 201247102 bud. It is preferably manually removed, for example, broken, stripped, cut or removed. The buds obtained in step (ix) are treated by at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one growth regulator, as desired. This treatment not only protects the "open wound" of the cut shoot from disease and pests, but also supports physiological effects. Suitable and preferred fungicides, insecticides and growth regulators are shown below. Among the agents, it is particularly preferred to use a fungicide (such as apigenin and especially kekemin) which also has a physiological action (especially plant health and/or growth promoting action). In insecticides, it is preferred. Treated by at least one GABA antagonist, preferably by at least one fungicide (specifically, quercetin fungicides, especially 100 grams of sensitizer) and/or at least one insecticide (specifically Preferably, the GABA antagonist, especially fipronil, and/or at least one growth regulator is treated by at least one fungicide (specifically, a scorpion fungicide) Especially In a specific embodiment, the step (χ) is carried out. The new shoots obtained in the step (ix) or (X) are then planted in a growth medium [step (xi)]. a day or a mixture of the substrate 4, examples of which are soil, clay, sand, mud, small wood, cellulose, decaying organic residues, insects to stone, coconut fiber and the like, and Such as a mixture of soil, insect to stone or coconut fiber, in particular, the growth medium can also be a nutrient solution, such as containing growth factors, fertilizer, 163649.doc 201247102 buffer, ion exchange Agent, inorganic salt (such as 'calcium salt (for example, calcium nitrate, calcium sulfate, calcium hydrogen phosphate), magnesium salt (for example, magnesium nitrate, magnesium sulfate), potassium salt (for example, potassium dihydrogen phosphate 'potassium nitrate), iron Salt (for example, ferrous sulfate, iron sulfide) and micronutrients (for example, lithium salts such as lithium gasification; copper salts such as copper sulfate, zinc salts such as zinc sulfate; aluminum salts such as aluminum sulfate; nickel a salt such as nickel sulfate; a tin salt such as tin chloride An aqueous solution of a cobalt salt such as cobalt nitrate; boric acid, and the like, such as: ^MKnQp) nutrient solution and Hegrand (Hoag丨 八八至2 solution, and the like. The nutrient solution can be used by For example: an inorganic substrate (eg, expanded clay) stabilizes the form. Furthermore, the growth medium can be used as a soil in the field. In the step (xii) as needed, before, during and/or after planting the sprout At least one fertilizer and/or at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one growth regulator and/or at least one super adsorbent and/or promote growth The bacteria are treated with the growth medium. Suitable and preferred fertilizers, fungicides, killing agents, nematicides, growth regulators, super-adsorbents and growth-promoting bacteria are described in the following fungicides, in particular The system is treated by a fungicide (such as apigenin and especially kekemin) which also has physiological effects (especially plant health and/or growth promoting effects). Preferably, it is treated by at least one fungicide (specifically, 'halophilic fungicide, especially 100 grams of sensitizer') and/or at least one growth regulator. Or alternatively, the shoots may be treated with at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one growth regulator before and/or during planting. Suitable and preferred fungicidal I63649.doc -19· 201247102 Insecticides, nematicides and growth regulators are described in the context. In the case of fungicides, it is particularly preferred to use fungicides which also have physiological effects (especially plant health and/or growth promoting effects (for example, hair roots)) (such as eccentrics and especially kekemin). deal with. Preferably, the treatment is carried out by at least one fungicide (specifically, a serotonin fungicide, especially a gram sensitive), and/or at least one growth regulator. If the sprout is treated before planting, this step Simultaneously with step (X). In this case, the above description is applicable, that is to say, in the fungicide, particularly preferably by a fungicide which also has a physiological action (especially plant health and/or growth promoting action) (such as In particular, in the insecticide, it is preferably treated by at least one gaba antagonist. Preferably by at least one fungicide (specifically, a serotonin fungicide, especially a gram of sensitizer), and/or at least one insecticide (specifically, a GABA antagonist, especially a fenp Ni), and / or at least one growth regulator at ί. More preferably, it is treated by at least one fungicide (specifically, a muscarinic fungicide, especially a gram of sensitizer). In a preferred embodiment, step (xii)e is preferably carried out by at least one of the eclipse fungicides and, if desired, at least one fungicide and/or at least one fertilizer And/or at least "(4) insecticide and / or at least one nematicide and / or at least one growth regulator and / or at least one super adsorbent and / or growth-promoting bacteria to treat sprouts and / or raw nutrients. Suitable and preferred etoposide fungicides are shown below in the preferred J body.适宜 Appropriate growth medium is taken out in step (8), and the shoots are planted in a container containing the medium grown in a preferred embodiment. Shown above in "for 163649.doc

S -20. 201247102 The preferred growth medium in the container is selected from the group consisting of soil, clay, sand, mud, small broken wood, cellulose, decaying organic residues, insect to stone, nunzi fiber and the like. Mixtures, and more preferably soil, vermiculite or coconut fibres, in particular coconut fibres. The container can be made of a conventional material or a biodegradable material. The advantage of the biodegradable container is that the seedlings can be planted in the field, that is, the seedlings need not be removed from the container prior to planting. This advantage also lies in the possibility of planting with automatic or semi-automated planting machines. In a specific embodiment, a biodegradable container is used in step (iv). The biodegradable container is based on a biodegradable material, and the biodegradable material is preferably based on biodegradable polymer, temple powder, cellulose, cellulosic material, polylactic acid, raw rubber, paper, cardboard, and cellulose. Pulp, grass, bagasse, sawdust, natural fibers or mixtures thereof. In another embodiment, the container is a conventional (ie, non-special biodegradable) container after the sprout is planted in the growth medium and, if desired, by at least one fertilizer, at least one fungicide, at least An insecticide, at least a nematicide, at least one growth regulator, at least one super adsorbent, and/or a growth promoting bacteria, after treating the sprout and/or growth medium, allowing the sprout to be at a temperature of at least 15 C Growing seedlings. Preferably, the sprout is between 丄$ and 35C, more preferably 18 to 35 ° C, even more preferably 2 to 35 ° C, in particular, 22 to 35 C and especially 25 to 35 C, for example 25 to 32 ° C or 25 Seedlings are grown to a temperature of 30 eC or 25 to 28 ° C or 25 to 27 ° C. Preferably, the sprout is grown at 40 to 100%, more preferably 5 to 95%, even more preferably 7 to 163649.doc • 21 · 201247102 to 90% and, in particular, 7 to 85% moisture seedling. The required temperature is naturally achieved (for example, by allowing sprouts to grow in warm climates (e.g., in tropical climates), or by artificial equipment. Artificial equipment is for example, a greenhouse or a covering material. The growth medium can be transferred to the greenhouse, for example, to medium, or after planting, or can be insulated, for example, by covering a growth medium containing the sprouts with a suitable material (e.g., a sheet). In a preferred embodiment, the shoots are planted in a container containing growth medium, wherein the 5 liters are placed in a greenhouse or placed in a greenhouse after planting. In the greenhouse, the temperature is preferably from 15 to 35 < 5 (:, more preferably ^ to "force, even more preferably from 20 to 35 t, in particular, 22 to 35 ° c and especially 25 to 35 t, for example, 25 to The humidity in the range of 32 ° C or 25 to 3 ° t or 25 to 28 ° C or 25 to 27 ° C is preferably 40 to 100%, more preferably 5 to 95. /, or even more preferably 70 to 90./〇 and in particular, within the range of 7〇 to 8〇0/〇. In another preferred embodiment, the shoot is planted into a container or field containing growth medium (in this case, growth medium) Covering the container or field with one or more covering materials. The covering material is, for example, a textile felt and a cover foil specially used for agricultural insulation/protection, such as agricultural plastic sheets, preferably black sheets. , for example, a black sheet in the form of a sheet tunnel, or a wool felt. In a step (xiv) as desired, by at least one fungicide and/or at least one insecticide and/or at least one nematicide and / or at least one growth regulator and / or at least one hair root and / or growth-promoting bacteria are treated at a temperature of at least 15 ° C The seedling growth and / or other growth medium one or more times, for example, '1' 2 or 3, preferably one or two times "and suitable 163649.doc -22 ·

S 201247102, insecticides, growth regulators, hair roots, and fines to promote growth are described below. Herein, treating the seedlings means treating the seedlings or growth medium or both of the germination of the autogenous medium. If treated, at least one fungicide, at least one insecticide and/or at least one nematicide is used depending on (especially) pest pressure (type and strength). In a preferred embodiment, step (xiv) is performed. Preferably, at least one fungicide and/or at least one GABA antagonist is used to kill the insecticide and, if desired, also by at least one different fungicide (ie, with the eccentric Different fungicides and/or at least one fertilizer and/or at least one different insecticide (ie, an insecticide different from the GABA antagonist) and/or at least one nematicide and/or at least one growth regulator And/or at least one supersorbent and/or growth promoting bacteria to treat the seedlings and/or their growth medium. Suitable and preferred etoposide fungicides are shown below. The preferred one is Bai Kemin. Preferably, the GABA antagonist is selected from the group consisting of acetoprole, endosulfan, vaniliprole, pyrafluprole, pyriprole 'as in formula II Phenyl. Specific compound

163649.doc -23· 201247102 wherein R^c^c4-alkyl or (^ to (: 4-haloalkyl; or an agriculturally acceptable salt thereof; and a phenylpyrazole compound of formula III)

Or an agriculturally acceptable salt thereof. 1 is preferably an ethyl or trifluoromethyl group. Particularly preferred is Fenpney. More preferably 'by at least one opiate fungicide and, if desired, by at least one different fungicide and/or at least one fertilizer and/or at least one insecticide and/or at least one Treating the seedlings and/or their growth medium with a nematode agent and/or at least one growth regulator and/or at least one supersorbent and/or growth promoting bacteria, wherein the at least one insecticide is preferably a GABA antagonist, especially Finkney. Suitable and preferred eosinophilic fungicides are shown in the following order. The specific preferred one is Bai Kemin. The seedlings that have been grown from the sprouts are planted to the field 1 to 12 Torr, preferably 25 to 1 day after the sprouts are planted in the growth medium (although only when the growth medium is not the field). At this point in time, the seedlings have essentially had a length of Μ to SO cm. More preferably after planting the shoots in the growth medium 163649.doc

S -24- 201247102 25 to 80 days 'Even better 4 to 7 days ^ Specially Lu Lu, 50 to 70 days and specific Luzhi, 60 ± 5 days, planted to the field is also considered to have borrowed From at least one fertilizer and / or at least one fungicide and / or at least - (four) insect cockroach / or at least - (four) nematode-like / or at least one growth regulator and / 岑 5 4, a 43⁄4 h 2 to fly to the evening super The adsorbent and/or the growth-promoting bacteria are treated and then planted. m These treatments can also be carried out during planting, for example, by means of strips. It is described below that the preferred fertilizers, fungicides, insecticides, nematicides, shengxian gang 八 八郎蜊王食调剂, super sorbent and growth promoting bacteria are described below. If the growth medium is field, 1 to 12 days after planting the sprout, preferably 25 to (10) days 'better 25 to 8 () days, or even better (10) money days to say '50 to 70 days and specific In other words, when 6〇±5 days, the seedlings that have emerged from the sprouts are exposed to the surrounding environment, that is, they are no longer insulated. For example, if it has been insulated by a cover material (eg, a cover sheet or wool felt), remove it. By at least one fertilizer and / or at least one fungicide and / or at least one insecticide and / or at least one nematicide and / or at least one after being planted in the field or after exposure to the surrounding environment The growth regulator and/or at least one supersorbent and/or growth promoting bacteria and/or at least one freshening polymer treat the seedlings or fields. Suitable and preferred fertilizers, fungicides, insecticides, nematicides, growth-regulating superabsorbents, growth-promoting bacteria and fresh-keeping polymers are described below. It can be artificially semi-automated or automatically planted into the field. For example, if biodegradable containers are used to grow new shoots and to allow seedlings to grow in them, then 163649.doc •25· 201247102 can be fully automated for planting. In this case, it is not necessary to take out the seedlings from the container before planting, thus allowing the planting machine to be used throughout the planting process. If a conventional container is used, the seedlings need to be taken out of the container before planting, and the process is generally carried out manually. The planting can then be automated. In the above treatment, the at least one fungicide is preferably selected from the group consisting of A) azoles selected from the group consisting of azoles and biphenyls. Bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, dilute alcohol-M, epoxiconazole, B Ring "etaconazole", fenbuconazole, fluquinconazole, flusiazole, powder. Flutriafol, hexaconazole, imibenconazole, ipconazole 'metconazole', myclobutanil, oxpoconazole , paclobutrazole, penconazole, propiconazole, prothiotoxin. Spontaneous (prothioconazole), simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, sterilized 0 sitting (triticonazole), uniconazole, 1-(4-a-phenyl)-2-([1,2,4]tris-s-l-yl)-cycloheptanol, cyazofamid ), imazalil, pefurazoate, prochloraz, triflumizol, benomyl, carbendazim

163649.doc -26- S 201247102 (carbendazim), fuberidazole, thiabendazole, ethaboxam 'etridiazole, hymexazole And 2-(4-Gas-phenyl)-N-[4-(3,4-dimethoxy-phenyl)-isoxazol-5-yl]-2-prop-2-ynyloxy - acetamamine; B) icophilin, which is selected from the group consisting of: azoxystrobin, dimoxystrobin, enestroburin, fluorosis vinegar ( Fluoxastrobin), kresoxim-methyl, metominostrobin, oressastrobin, picoxystrobin, pyraclostrobin, "pyraoxystrobin ), Pyrametostrobin, pyribencarb, trifloxystrobin, 2-(2-(6-(3-Ga-2-methyl-phenoxy)-5- Fluoropyrimidin-4-yloxy)-phenyl)-2-methoxyimino-N-mercapto-acetamide, 3-methoxy-2-(2-(Ν-(4- Methoxy-phenyl)-cyclopropane-carboxyiminothiolanyl)-phenyl)-acrylic acid '2-Ga-5-[1-(3-mercaptobenzyloxyimino)-ethyl]benzyl)amino decanoate and 2-(2-(3-(2,6-) Diphenyl)-1-indolyl-arylene-aminooxymethylmethyl)-phenyl)-2-methoxyimino-N-indenyl-acetamide; C) Carboxylic acid Amidoxime, selected from the group consisting of: benalaxyl, bendal-M, benodanil, bixafen, boscalid, wilt (carboxin), fenfuram, cycloheximide 163649.doc •27· 201247102 (fenhexamid), flutolanil, furametpyr, pyrazolam, iSOpyrazam Isotianil, kiralaxyl, mepronil, metalaxyl, methaul-Μ (mefenoxam), furanide (ofurace), eus Oxadixyl, oxycarboxin, D.0 penthiopyrad, sedaxane, tecloftalam, thifluzamide, tiadinil, 2 -amino-4-methyl-thiazol-5-mercaptoaniline, 2-gas-N-(l,l,3-trimethyl-oxime -4-yl)-nicotine decylamine, :^-(3·,4',5·-trifluorobiphenyl-2-yl)-3-difluorodecyl-1-indenyl-1H·吼0 Sodium-4-branched amine (fluxapyroxade), N-(4'-trifluoromethylthiobiphenyl-2-yl)-3-difluoromethyl-1-methyl- 1H-»Bizozol-4-decylamine, N-(2-(l,3-dimercapto-butyl)-phenyl)-1,3-didecyl-5-fluoro-1Η-pyrazole 4-nonylamine and N-(2-(l,3,3-tridecyl-butyl)-phenyl)-1,3·dimethyl-5·fluoro-1Η-pyrazole-4- Formamide, dimethomorph, flumorph, pyrimorph, flumetover, IL 0 flupicolide, fluopyram, seat Zoxamide, N-(3-ethyl-3,5,5-tridecyl-cyclohexyl)-3-indoleamine-2-yl-benzoguanamine, gappamine (carpropamid) ), dicyclomet, mandiproamid, oxytetracyclin, silthiofam, and N-(6-decyloxy-pyridin-3-yl)cyclopropane oxime Acid amide; 163649.doc • 28 -

S 201247102 D) Heterocyclic compounds selected from the group consisting of fluazinam, pyrifenox, 3" to 5, (4' gas-phenyl)-2,3-di Methyl-isox. Sit-3-yl] is more than 3,5 [5-phenyl-phenyl)-2,3-dimethyl-iso-.定定,q 2,3,5,6-甲气-努^气-赛普洛芬竦莫减•派林尼埤莫赛福宁护宁宁菌菌灵芬福福四气-4-曱Burnt stone yellow base ratio, 3,4,5-trichloro-n ratio 0 _2 6 nitrile, Ν-(1-(5 - desert-3 - chloro-0 ratio bit-2-yl)-ethyl )-2 4--Acoustic amine, N-[(5->odor-3 - gas-° ratio -2-定-2-yl)-methyl)_2 * Final test guanamine, bupirimate (cyprodinil), diflumetorim (fenarimol), fermizone, (mepanipyrim), nitrapyrin (nuarimol), pyrimethanil (triforine) 'fenpiclonil ), (fludioxonil), aldimorph (dodemorph), carbendazim acetate, (fenpropimorph), tridemorph, fenpropidin, fluoroimid ), Iprodione, chlozolinate, procymidone, vinclozolin, famoxadone, fenamidone, flutianil , octhilinone, probenazole, 5-amino-2-isopropyl-3-oxooxy-4-o-indolyl-2,3-dihydro-pyrazole-1- S-allyl thioformate, a-cixolar-S-methyl, β-bend | "Sodium ruthenium 163649.doc -29- 201247102 (amisulbrom), enilazin (anilazin) , blasticidin-S, captafol, captan, chinomethionat, dazomet, debacarb, diclomezine ), difenzoquat, wild swallow-methylsulfate, fenoxanil, Folpet, oxolinic acid, piperalin, propionol Proquinazid), pyrrox (pyr0qUil〇n), quinoxyfen, triazoxide, tricyclazole, 2-butoxy-6-e-3-propyl gram-4 -ketone, 5-chloro-1-(4, 6-dimethoxy-pyrimidin-2-yl)-2-methyl-1H-benzimidazole, 5·gas-7-(4-methylpiperidin-1-yl)-6-(2,4 ,6-trifluorophenyl)-[1,2,4]triazolo[l,5a]pyrimidine and 5-ethyl-6-octyl-[1,2,4]triazolo[l,5 -a]pyrimidin-7-ylamine; E) urethanes selected from the group consisting of: ferbam, zinc, mancozeb, maneb, man Metam, metasulphocarb, metiram, propineb, thiram, zineb 'ziram, volk (benthiavalicarb), pyributicarb, diethofencarb, iprovalicarb, iodocarb, propamocarb, propk-hydrochloride, thiophane (prothiocarb), valifan (valiphenal) and N-(l-(i-(4_ 芳163649.doc -30-

S 201247102 phenyl-phenyl) ethanesulfonyl)-butyl-2-yl)amino decanoic acid _(4-fluorophenyl) ester; F) other active compounds 'these are selected from the group consisting of: - terpenoids, pulse, dodine, doxy free test, guazatine, gram-ethyl acetate, iminoctadine, bis-octylamine-triacetate, biguanide Aminoctadine-tris (albesilate); - Antibiotics: kasugamycin, kasugamycin-hydrochloride, hydrate, streptomycin, polyoxine , validin A (a); benzene derivatives: binapacryl, dinobuton, dinocap, meptyldinocap, nitroxal -isopropyl), tetrakis nitrobenzene (tecnazen), - organometallic compounds: fentin salts, such as fentin-acetate, fentin chloride or triphenyl hydroxide Fetin hydroxide; - sulfur-containing heterocyclic compounds: dithianon, isoprothiolane; - organic complex : edifenphos, fosetyl, fossett-ming, iprobenfos, linoleic acid and its salts, pyrazophos, tolclofos-methyl Organic gas compound: chlorothalonil, Yifa 163649.doc 31 201247102 spirit (dichlofluanid), dichlorophen, flusulfamide, hexachlorobenzene, penmone (pencycuron) ), pentachlorophenole and its salts, phthalide, quintozene, thiophanate-methyl 'tolylfluanid', N-(4) - gas-2-stone schyl-phenyl)-N-ethyl-4-methyl-benzenesulfonamide; - inorganic active: Bordeaux mixture, copper acetate, copper hydroxide, copper oxide, alkali Copper sulphate, sulfur; - other · biphenyl, bromoopol, cyflufenamid, chloroneb, cymoxanil, dicloran, tetrachloro Nitrobenzene (tecnazene), diphenylamin, metrafenone, Mildiomycin, oxin-copper, pr〇hexadione calcium, spiroxamine, t〇iyfiuanid, N-(ring Propyl methoxyimino-(6-difluoro-decyloxy-2,3-difluoro-phenyl)-indenyl-2-pyridylacetamide, Ni_(4-(4-gas) -3-trifluoromethyl-phenoxy)-2,5-diindenyl-phenyl)·Ν_ethyl_N•mercaptomethyl hydrazine, ν, _(4·(4-fluoro-3- Trifluoromethyl-phenoxy)_2,5-didecyl-phenyl)_Ν_: benzyl-hydrazino-hydrazino, ν,·(2-mercapto-5-trifluoromethyl-4-( 3-trimethyl-stone-propoxy-propyl)-N-ethyl_N_methylformamidine N'-(5-difluoromethyl-2-mercapto-4-(3-tri) Mercaptoalkyl-propoxy)-phenyl)-N-ethyl-N-indenyl-lung, fluorenyltrifluoro-163649.doc

S •32- 201247102 decyl-pyrazol-1-yl)-ethinylpiperidine-4-yl}-thiazole_4-decanoic acid, fluorenyl-(1,2,3,4-tetrahydro- Naphthyl-diyl) decylamine, 2_μ_[2·(5_mercapto-3-trifluoromethyl-η-pyrazole-1 1 yl)-ethenyl]-piperidine-4-yl-thiazol-4- Formic acid, mercaptotetrahydro-naphthalen-1-yl)-nonylamine, acetic acid-6-t-butyl-8-fluoro-2,3-diyl-fluorene- 4 - Acetic acid 6-tert-butyl_8_fluoro-2,3-dimethyl-quinoline-4'; and G) biological control agent. Biological control is defined as the reduction of the number of pests by natural enemies and generally involving human active participation. The most common biological control of plant diseases is based on the antagonism of BeΑ. There are several types of fungal biological control machines, including the production of antifungal antibiotics, nutritional competition and root colonization. Suitable biological control agents are selected from non-pathogenic bacteria, preferably selected from the group consisting of Pseudomonas fluorescens, Pseudomonas putida, Streptomyces griseus, Streptomyces. Ochraceisleroticus), Streptomyces graminofaciens, Streptomyces corchousii, Streptomyces spiroverticillatus, Streptomyces griseovirdis, Streptomyces hygroscopicus, Bacillus subtilis (Streptomyces hygroscopicus) Bacillus subtilis), Bacillus cereus, Bacillus mycoides, Bacillus pumilus, Bacillus licheniformis (Bacillus 163649.doc -33- 201247102 licheniformis), Suyun Golden Bud Bacillus thuringensis and metabolites produced from the bacteria; non-pathogenic fungi, preferably selected from Trichoderma spp., Trichoderma harzianum, Trichoderma viridae, wax Verticillium lecanii, Use of Sporides mium sclerotiorum and Zygomycetes, and metabolites produced from the fungus, plant extracts of myric acid, Reynoutria sachalinensis; and plant defense inducers, preferably Preferably, the harpin is at least one fungicide selected from the group consisting of cycloxazole, chlorhexidine, ezine, flurazepam, flucarbazole, fluconazole, cyanoxazole, myclobutanil , pentobacteria ° sitting, ring. Sitting, propargyl spit, three. Take ketone, three. Alcohol, penta-isopropanol, tetrafluoroetherazole, sterilized azole, poker, racer, free, carbendazim, ethaboxam, atmomin, ether oxystrobin, fluoxastrobin, fluorobenzene Pyraclostrobin, kexinxin, faecal amine, oxystrobin' baikemin, trifluoro-sensitive, baisaifen, leucoside, dexamethasone, cycloheximide, chlorhexidine, pyrazol Amine, dextromethorphan, furosemide, dawhen, flumorph, fluopyram (flurazepam), dexamethasamine, gupamine, dipropionamide, fluazinam ), 赛普洛, 芬瑞莫, 灭派林, 派美尼, Saifuning's 护宁宁, carbendazim, fenfen, thirteen morpholine, fenpropidin, yiputong, kekening , killing the same with azole, chlorpyrifos, propoxyquinoline, S-mercapto-azepene, tetradane, sterilized dan, carbendazim, vebufen, 5-ethyl-6-octyl- [1,2,4] monoazolo[1'5-a]pyrimidine_7-ylamine, zinc-manganese-purine, rotten, sulfhydryl-zinc, thiram, acesulfame, voltose Lika 163649.doc

-34- S 201247102 (benthiavalicarb)), plucker, nitrile sulphide, triptyril "(3)^...salt, forsyth, forsythia-aluminum, HjO3 and its salts, chlorthalcmil , Yifaling, methyl chlorhexate, copper acetate, copper hydroxide, copper oxychloride, copper sulphate, sulphur, gram, fentanyl, spiroxamine and Bacillus subtilis and their metabolites. At least one fungicide is a combination of at least one of a muscarinic fungicide or a Bacillus subtilis and/or a metabolite thereof or a fungicide thereof. Preferably, the eclipse is selected from the group consisting of Tomin, epothilone, fluoxastrobin, flufenpyram, kexinxin, acesulfame, oxystrobin, baikemin and trifluoro-sensitive. In particular, icophilic fungicidal fungicide The agent is 100%. In the above treatment, the at least one insecticide is preferably selected from the group consisting of a) a pyrethroid compound selected from the group consisting of acrinathrin, allethrin, and d-cis. -trans arylene, d-trans arylene, bifenthrin, bioallethrin, S-cyclopentenyl bio-enyne , bioresmethrin, cypromethrin (CyCl〇pr〇thrin), cyfluthrin, β-saiconin, cyhalothrin, λ-saironin, γ- Cyronin, cypermethrin, α-赛灭宁, β-赛灭宁, Θ-赛灭宁, ξ-赛灭宁, cyanidin g (cyphenothrin), 灭宁宁 (deltamethrin) ,empenthrin, esfenvalerate, etfenfenx, fenpropathrin, fenvalerate, 赛赛宁163649.doc •35- 201247102 (flucythrinate ), flumethrin, flu-valfluinate, halfenprox, imiprothrin, metofluthrin, permethrin ), phenothrin 'prallethrin, profiuthrin, pyrethrin (pyrethrum), pyrethrin (resmethrin), Silafluofen, tefluthrin, tetramethrin, tralmethrin, and tetrakierene Sfluthrin); b) a nicotinic acid receptor agonist/antagonist compound selected from the group consisting of acetamiprid, bensultap, cartap hydrochloride, clothianidin, ° Dinotefuran, imidacloprid, thiamethoxam, nitenpyram, nicotine, spinosad (ectopic agonist), ethyl spinosyn ( Spinetoram), thiacloprid, thiocyclam, thiosultap-sodium, and AKD 1022; c) GABA gated chloride channel antagonist compound selected from Chlordane, endosulfan, γ-HCH (lindane), ethiprole, fipronil, pyrafluprole and bipro (pyriprole); d) a gas channel activator selected from the group consisting of abamectin, emamectin benzoic acid vinegar, milbemectin, and lepimectin;

163649.doc -36- S 201247102 e) Chitin biosynthesis inhibitors: (el) benzamidine ureas: bistrifluron, chlorfluazuron, diflubenzuron, Flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, noifurumuron, teflubenzuron, kill Triflumuron. More preferably, the at least one insecticide is selected from the group consisting of fentanyl, arsenic, chlothianidin, idacaine, acesulfan, aba; butyl and defodron, and specific fentanyl . In the above treatment, the at least one nematicidal agent is preferably selected from the group consisting of - an antibiotic nematicide, such as abatatin; - a plant nematicide, such as carvacrol; - a soap tree (Quillaja) or a acacia ( Gleditsia) extracts; - saponines; - amino phthalate nematicides, selected from the group consisting of ridding, carbofuran, carbosulfan and cloethocarb - an amine phthalate nematicide selected from the group consisting of alanycarb, aldicarb, aldoxycarb, euridine and tirpate; - fumigant Nematicide, selected from dithioether and decyl bromide; - organic nematode insecticide · 163649.doc -37· 201247102 - organophosphate nematicide, selected from diamidafos ; fenamiphos; fosthietan and phosphamidon; - organothiophosphate nematicides selected from cadusafos, chlorpyrifos, wire-disc (dichlofenthion), dimethoate, ethoprophos, fengfusong (fen Sulfosion), fosthiazate, heterophos, isamidofos, isazofos, phorate, phosphocarb, terbufos, insect Wire dish (thionazin) and triazophos; - thiophosphonite nematicide selected from imicyafos and mecarphon; and - unclassified nematicide, selected from Acetylene nitrile, benclothiaz, chloropicrin, mailong, DBCP, DCIP, 1,2-dipropane, 1,3-dipropene, fluthiasulfone (fluensulfone) ), Kushiro, Weibai, 曱 蛾 moth, methyl isothiocyanate and xylenol. In particular, the at least one nematicide is abatatin. In the above treatment, the growth promoting bacteria is preferably selected from the group consisting of azospirillum, az〇t〇bacter, azomonas, bacillus, Beyjerinckia, burkholderia, clostridium, Cyan〇bacteria, Enterobacter

163649.doc -38· S 201247102 (enterobacter), erwinia, gluconobacter, klebsiella and streptomyces bacterium 0 is better, which promotes growth The bacteria are selected from the group consisting of Azospirillum amazonense, Her ba spirillum seropedicae, Herbaspirillum rubrisubalbicans, Burkholderia tropica, and Gluconacetobacter diazotrophicus 'Pseudomanas fluorescens, Pseudomonas putida, Streptomyces griseus, Streptomyces ochraceisleroticus, Streptomyces sp., Streptomyces corchousii Streptomyces spiroverticillatus, Streptomyces griseovirdis, Streptomyces hygroscopicus, Bacillus subtilis, Bacillus cereus, Bacillus licheniformis, Bacillus pumilus, Bacillus licheniformis and Bacillus thuringiensis. In the above treatment, the at least one growth regulator is preferably selected from the group consisting of fluorenylcyclohexanone, such as prohexadione, cyclamate-Ca, trinexapac or anti-pour ethyl ester ( Trinexapac ethyl); mepiquat chloride and chl〇mequatchloride mepiquat. More preferably, the at least one growth regulator is selected from the group consisting of nonylcyclohexanedione such as cyclohexanoic acid, cyclohexanoic acid-Ca, anti-pour acid or anti-pour ethyl ester, and specifically selected from the group consisting of a cyclic acid -Ca and anti-pour ethyl ester. Preferably, the at least one hair rooting agent is selected from the above-mentioned fungicidal fungicidal agents. 39-163649.doc 201247102 Agents, in particular, fluorobenzamine and chloramine; the above-mentioned final acid receptor agonist / antagonists 'specifically' cotinidine, edamine and cytosine; auxin 'such as 4-CPA, 2,4-D, 2,4-DB, 2,4-DEP, drip propionic acid ( Dichlorprop), fenoprop, iaa, IBA, naphthylamine, α-naphthylacetic acid, 1-naphthol, naphthyloxyacetic acid, potassium naphthenate, sodium naphthenate and 2,4,5-anthracene Gibberellins, gibbere Uic acid, cytokinins (eg, 2iP), benzyl adenine, 4-hydroxyphenylethyl alcohol, kinetin, and glutamic acid; and humic acid , soap tree or soap extract, saponin, biological control agent and plant defense inducer. Suitable fertilizers are those specially used for the cultivation of sugarcane plants, such as NPK fertilizer, ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, plant-derived products (such as cereal meal, bark meal, wood meal, nut meal) a mulch covering, and the like. The at least one super sorbent is preferably a super sorbent polymer having an adsorption capacity of at least 1 〇〇g of deionized water/ig polymer. Super sorbent polymer is well known Synthetic organic polymer, which is solid and hydrophilic, insoluble in water, and can adsorb multiple times its equivalent weight of water or aqueous solution, thereby forming an aqueous polymer gel, which can be nonionic or ionic. Copolymers. Suitable supersorbent polymers are known, for example, from US 4,417,992, US 3,669,103, WO 01/25493, and WO 2008/031870. They are also commercially available from the market, for example, from France. SNF SA. purchases a product under the trade name Aquasorb® (for example, 3500 S) or a product from the BASF SE purchaser 4 named Luquasorb® 'for example, Luquasorb® 1010,

Luquasorb® 1280, Luquasorb® 1060, Luquasorb® 1160, 163649.doc 201247102

Luquasorb® 1061 and HySorb®. Preferably, the wound protection material is selected from non-toxic inorganic and organic film forming or coating polymers such as super adsorbents, super adsorbent treated polymers (eg Luquasorb® from BASF), aliphatic-aromatic copolymerization. Esters (eg Ecoflex® from BASF), preservative polymers (eg FreshSeal® from BASF), waxes, soluble glass and natural swelling substances (eg 'Tingui (Magonia pubescens)) seed coat preparations, especially from Magnolia Powder of seed coat or, more specifically, 'gel formed from such powders'. Among these, 'the preferred ones are FreshSeal® and gels prepared from Magnolia seed coat powder. Such fungicides, insecticides, nematicides, growth regulators and hair roots are generally used in the form of ready-to-use preparations. Hereinafter, a suitable ready-to-use preparation containing at least one fungicide, insecticide, nematicide, growth regulator or hair root preparation (hereinafter referred to as "active ingredient") will be described. In a ready-to-use preparation, the active ingredient may be in a suspension, emulsification or dissolved form. The form of application will depend entirely on the intended use. The active ingredient may be administered in the form of its formulation or in the form of a preparation prepared from the formulation, eg in the form of a direct sprayable solution, powder, suspension or dispersion, including a highly concentrated aqueous, oily or other suspension or dispersion. , Emulsions, oil dispersions, pastes, dusts, compositions or granules for spreading, typically applied by spraying, atomizing, spraying, spreading or concentrated water. The form and method of application will depend on the intended use; in each case, it should be such that the active compound is as evenly distributed as possible. According to an embodiment of the ready-to-use preparation containing the active ingredient, the preparations comprise - I63649.doc 201247102: a multi-discussion or solid carrier, if appropriate, & containing a surfactant, and if appropriate 'including other specifically for blending An adjuvant for crop protection agents. The formulation of such formulations is known to those skilled in the art. The aqueous application form can be prepared, for example, from an emulsion concentrate, a suspension, a paste, or a water-dispersible granule, by adding water. In order to prepare an emulsion 1 or an oil dispersion, the original form or the active compound dissolved in an oil or solvent can be homogenized in water by a wetting agent, an adhesive, a dispersing agent or an emulsifier. Alternatively, a concentrate composed of an active substance, a wetting agent, a binder, a dispersing agent or an emulsifier, and if necessary, a solvent or an oil may be prepared, and such concentrates are suitably diluted with water. The concentration of the active ingredient in the ready-to-use preparation can vary over a wide range. In general, it is between 0 0001 and 10%, preferably between 〇ι and 1% (% by weight of the total active compound, based on the total weight of the ready-to-use formulation). The active ingredient can also be used successfully in ultra low volume methods (ULV), which can employ formulations containing more than 95% by weight of active compound, or even application of active ingredients without additives. Various types of oils, wetting agents, auxiliaries, bactericides and/or fertilizers can be added to the active ingredient, and, if appropriate, added until before use (large tank mixing). These preparations may be mixed with the active ingredients employed in a weight ratio of from 1:1 Torr to 1 :1, preferably from 1:1 Torr to 1 Torr. The auxiliary system is, for example, a modified organic polyoxyalkylene, for example, Break

Thru S 240®; alcohol alkoxides, for example, Atplus 245®, Atplus MBA 1303®, Plurafac LF 300® and Lutensol ON 30®; EO- t63649.doc • 42- s 201247102 PO block copolymers, for example, Plur 〇nic RPE 2〇358 and Genap〇1 B®, alkanol ethoxylates, for example, Lutens〇1 χρ 8〇@; and sodium dioctylsulfosuccinate 'for example, LeophenRA®. The formulations are prepared in a known manner, for example, by supplementing the active ingredient with a solvent and/or carrier, if desired, using surfactants, i.e., emulsifiers and dispersing agents. Solvents/carriers suitable for this purpose are essentially: • water, aromatic solvents (eg, Solvesso products, xylene), paraffins (eg 'mineral oil fractions'), alcohols (eg methanol, butanol, pentanol) , benzyl alcohol), ketone (for example, cyclohexanone, methyl hydroxybutyl ketone, diacetone alcohol, isopropylideneacetone, isophorone), lactone (for example, γ-butyrolactone), pyrrolidone ( Pyrrolidine _, N_mercaptopyrrolidone, N-ethylpyrrolidone, N-octylpyrrolidone), acetate (diacetate), diol, dimercapto fatty acid decylamine, fatty acid and fatty acid ester In principle, a solvent mixture can also be used. - Carriers such as ground natural minerals (eg kaolin, clay, talc, chalk) and ground synthetic minerals (eg subdivided cerium oxide, cerium); emulsifiers such as nonionic and Anionic emulsifiers (such as 'polyethylene oxide fatty alcohol ethers, alkyl sulfonates and aryl sulfonates) and dispersants 'such as lignin sulfite waste liquid and methyl cellulose. Suitable surfactants are lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalene acid, alkyl aryl acid, sulfuric acid alkyl ester, acid-base vinegar, fatty alcohol sulfate, fatty acid and Sulfated fatty alcohol glycol test for metal sleep, soil test metal salt and salt recorded; other acidified naphthalene and naphthalene derivatives and road 163649.doc •43· 201247102 condensate, naphthalene or naphthalenesulfonic acid Condensate of phenol and furfural, polyethylene oxide octyl phenol ether, ethoxylated isooctyl phenol, octyl phenol, nonyl phenol, alkyl stupid = polyglycol ether, tributyl phenyl poly Glycol ether, trisyl-based polyglycol ether, alkyl aryl polyether alcohol, alcohol and fatty alcohol cyclohexane condensate, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylated poly Epoxy propylene, lauryl alcohol polyglycol ether acetal, sorbitol ester, lignin sulfite waste liquid and sulfhydryl cellulose are suitable for preparing direct sprayable solution, emulsion, paste or oil dispersion Mineral oil with medium to high boiling point, such as kerosene or diesel, other coal tar and plant and animal source oil; aliphatic 'ring And aromatic hydrocarbons such as toluene, diphenylbenzene, paraffin, tetrahydronaphthalene, alkylated naphthalene or its derivatives, methanol, ethanol, propanol, butanol, cyclohexanol, cyclohexanone 'isopropylidene Acetone, isophorone, strong polar solvent, such as diterpene hard, 2 _. More than π each of the ketone, fluorenyl-pyridyl pyrrolidone, butyrolactone or water. The powder, the composition for spreading and the dust can be prepared by mixing or co-milling the active ingredient with a solid carrier. Granules, for example, coated granules, impregnated granules, and homogeneous granules can be prepared by binding the active ingredient to a solid carrier. Solid carrier systems, for example, mineral soils such as tannins, silicates, talc, kaolin, red orange stones, limestone, lime, chalk, red basalt, loess 'clay, dolomite, shixia, calcium sulphate, Magnesium sulfate, magnesium oxide; ground synthetic materials, fertilizers, such as 'such as 'ammonium sulfate, ammonium phosphate, ammonium nitrate, urea and plant products, such as glutinous meal, bark coarse powder, wood meal and nutshell meal, Cellulose powder and other solid carriers. 163649.doc -44 -

S 201247102 Formulations for sprout treatment may further comprise a binder and/or a gelling agent and optionally a coloring agent. Generally, the formulations comprise from 0.01 to 95% by weight, preferably from 90% to, in particular, from 5 to 5% by weight of active ingredient. Herein, the purity of the active ingredient used is from 9% to 1%, preferably from 95% to 10,000% (by NMR spectrum). After two to ten times dilution, the formulation for treating the sprouts may comprise from 0.01 to 60% by weight, preferably from 5% to 5% by weight, of the active ingredient in the ready-to-use preparation. Examples of formulations are: 1. Products for dilution in water I) Water-soluble concentrates (SL, LS) Dissolving 1 part by weight of the active compound in 90 parts by weight of water or water-soluble solvent . Or 'add a humidifying agent or other adjuvant. When diluted in water, the active compound will dissolve. The resulting formulation contained 1% by weight of active ingredient. II) Dispersible Concentrate (DC) 20 parts by weight of the active compound is dissolved in 70 parts by weight of cyclohexanol' and 10 parts by weight of a dispersing agent, for example, polyethylene, is added. More than σ each bite ketone. The active ingredient content was 20% by weight. When diluted in water, a dispersion is obtained. III) emulsifiable concentrate (EC) 15 parts by weight of the active compound is dissolved in 75 parts by weight of dimethyl benzene, and added with decyl benzoic acid and castor oil ethoxylate (respectively 163649 .doc • 45- 201247102 in 5 parts by weight). The active ingredient content was 15% by weight. When diluted in water, an emulsion is obtained. IV) Emulsion (EW, hydrazine, ES) 25 parts by weight of the active compound is dissolved in 35 parts by weight of bismuth benzene, 'addition of calcium dodecylbenzene sulfonate and phthalic acid ethoxylate (5 parts respectively) weight ratio). This mixture was added to 30 parts by weight of water using an emulsifier (Ultraturrax) to prepare a homogeneous emulsion. The active ingredient content was 25% by weight. When diluted in water, an emulsion is obtained. V) Suspension (sc, 〇D, FS) pulverize 2 parts by weight of the active compound in a ball mill, add 1 part by weight of dispersant, wetting agent and 7 parts by weight of water or organic The solvent 'forms a homogeneous suspension of the active compound. The active ingredient content is 2 〇 weight 0/. . When diluted in water, a stable suspension of the active compound is obtained. VI) Water-dispersible and water-soluble particles (WG, SG) uniformly grind 50 parts by weight of active compound, add 5 parts by weight of dispersant and wetting agent, and use technical equipment (for example, extrusion, spraying The mist tower, fluidized bed) is made of water-dispersible or water-soluble particles. The active ingredient content is 50% /" s. When diluted in water, a stable active compound dispersion or solution is obtained. νπ) Water dispersible or water soluble powder (WP, SP, SS, ws) in the rotor tweezers Grinding 75 parts by weight of the active compound, adding 25 parts by weight to the dispersant, wetting agent and silicone. The active ingredient content is 75% by weight. When diluted in the middle, the stable active compound dispersion or Solution. 163649.doc

-46- S 201247102 VIII) Gel Formulation (GF) Grinding 20 parts by weight of active compound, part by weight of dispersant, 1 part by weight of gelling agent and 70 parts by weight of water in a ball mill Or an organic solvent to form a uniformly dispersed suspension. When diluted in water, a stable suspension of the active compound is obtained. 2. Products for direct application IX) Dust (DP, DS) 5 parts by weight of the active compound are finely ground and uniformly mixed with 95 parts by weight of fine granular kaolin. A dust having an active ingredient content of 5% by weight was formed. X) Granules (GR, FG, GG, MG) 0.5 parts by weight of the active compound are finely ground and combined with 95.5 parts by weight of the carrier. Existing methods are extrusion, spray drying or fluidized beds. Directly applied granules having an active ingredient content of 0.5% by weight are formed. XI) ULV solution (UL) 10 parts by weight of the active compound is dissolved in 9 parts by weight of an organic solvent (e.g., 'xylene). A direct application product having an active ingredient content of 10% by weight is formed. Formulations suitable for the treatment of sprouts, for example: 1 soluble concentrate (SL, LS) III emulsifiable concentrate (EC) IV emulsion (EW, EO, ES) V suspension (SC, OD, FS) VI water Dispersible and Water Soluble Particles (WG, SG) 163649.doc • 47· 201247102 VII Water Dispersible and Water Soluble Powder (WP, SP, ws) VIII Gel Formulation (GF) IX Dust and Dust Powder ( DP, DS) A better blending system for handling sprouts! ^Formulations β _ In general, such formulations comprise from 1 to 800 g/i of active compound, from 1 to 2 g/i of a wetting agent, from 0 to 200 g per ounce of antifreeze, to 4 The binder of 〇〇g/i, which is a coloring agent (pigment and/or dye) and a solvent of 2 (eight) g/Ι, preferably water. Preferred active compound FS formulations for the treatment of new shoots generally comprise from 5 to 80% of the active compound, from 5 to 5% of the wetting agent, from 5 to 15% of the dispersing agent, from 0.1 to 5%. Thickener, 5 to 20% antifreeze, 1 to 2% antifoam, 0 to 1 5% adhesive or adhesive, 75% filler/vehicle, and 〇.〇1 Up to 1 °/. Preservatives. Suitable wetting agents and dispersing agents are in particular the abovementioned surfactants. Preferably, the wetting agent is an alkylnaphthalenesulfonate such as diisopropyl or diisobutylnaphthalenesulfonate. Preferred dispersants are nonionic or anionic dispersants or mixtures of nonionic or anionic dispersants. Suitable nonionic dispersants are, in particular, ethylene oxide/propylene oxide block copolymers, alkylphenol polyglycol ethers and tristyrylphenol polyglycol ethers, such as polyoxyethylene octylphenol ethers, Ethylene oxide octyl phenol, octyl phenol, nonyl phenol, alkyl phenol polyglycol ether, tributyl phenyl polyglycol ether, trisyl polyglycol ether, alkyl aryl polyether alcohol Alcohol and fatty alcohol/ethylene oxide condensate, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylated polyoxypropylene, lauryl alcohol polyglycol ether acetal, sorbitol ester and methyl cellulose. Suitable anionic dispersant, in particular, lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid 'alkylarylsulfonic acid, alkyl sulfur

163649.doc -48· S 201247102 Alkali metal salts, soil test metal salts and ammonium salts of acid, alkyl sulfonic acid, fatty alcohol sulfuric acid, fatty acid and sulfated fatty alcohol glycol ether; others are aryl sulfonic acid / 曱A condensate of an aldehyde, such as a condensate of a sulfonated naphthalene and a naphthalene derivative with formic acid, a condensate of naphthalene or naphthalenesulfonic acid with phenol and formaldehyde, a lignin hydrochloride, a lignin sulfite waste liquid, a fluorene-based fiber Phosphorylated or sulfated derivatives and polyacrylates. Suitable for use as an antifreeze is in principle all substances below the melting point of water. Suitable antifreeze agents include alkanols such as methanol, ethanol, isopropanol, butanol, ethylene glycol, glycerin, diethylene glycol and the like. Suitable thickeners are all materials which can be used for this purpose in agrochemical compositions, for example, cellulose derivatives, polyacrylic acid derivatives, xanthan gum, modified clays and finely divided vermiculite. Suitable as defoamers are all defoamers that are specifically formulated for the formulation of agrochemically active compounds. Particularly suitable are ketone antifoaming agents and magnesium stearate. Suitable preservatives are all preservatives which can be used in agrochemical compositions for such purposes. Examples thereof may be referred to as di(tetra) (diehl_phene), iso-sialin (iS〇thiaZ〇lene), such as U-benzoisoxanthone, 2-methyl-2-indolyl (tetra)·3, hydrochloride, 5' gas-2-(4•chlorof-yl)_3(2Η)·iso-seat steel, 5-gas·2-methyl-2Η-iso.塞吐_3_嗣, 5-气_2_methyl_2Η-iso-s- 3 ketone, 5-chloro-2-methyl·2Η-isothiazole _3-ketone hydrochloride, 4,5-dichloro · 2-cyclohexyl-4-isoindole. 3,4,5-dichloro_2•octyl_2η isothia^3′, 2-methyl-2Η-iso-salt-3, ' 2 曱 Η 2 Η 嗟 嗟 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ ‘ 163649.doc -49- 201247102 Adhesives/adhesives may be added to improve the adhesion of the active ingredient to the treated shoots. Suitable adhesives are E〇/P〇 type block copolymer surfactants, including polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene Polyethylenediamine, polyethylenediamine, polyethylenediamine (Lupas(R), p〇lymin8), polyacrylic acid and copolymers derived from such polymers. Suitable compositions for treating soil mites include granules which can be applied as granules or impregnated fertilizer granules and sprayed before or after excavation; spray applied to the soil in the form of Li" for treating plants, in particular Suitable compositions of the aerial parts of plants, especially "open wounds" and seedlings (especially seedling leaves), include spray liquid, dust and particulates, preferably sprayed with liquid. Formulations suitable for direct application of spray solutions: 1 Soluble concentrate (SL, LS) Π Dispersible concentrate (DC) III emulsifiable concentrate (EC) IV Emulsion (EW, E〇) V Suspension (SC) VI water dispersible and water soluble particles (WG) VII water dispersible and water soluble powder (WP, sp) (before planting) can be used to treat new shoots, some special treatment or coating The method 'such as, but not limited to, using the active ingredient or its formulation, coating, soaking, soaking, for example, can be used in a device suitable for this purpose, with the original form of the desired amount 163649.doc 201247102 The active ingredient formulation after dilution with water is treated for treatment, for example, in a mixing apparatus for solid or solid/liquid mixed mate until the composition is evenly distributed over the sprout. If appropriate, then carry out the drying operation. The supersorbent is typically applied to the growth medium or field by mixing with the growth medium or soil or by applying the desired amount of supersorbent to the hole for the sprout or seedling. The latter method is preferred for applying a superabsorbent to the field. By the method of the present invention, when the sugar cane plant is propagated, the required area is significantly reduced compared to the conventional method and the Plene® method, because a growing sugar cane plant will produce an increased amount of sprouts. Compared with the propagation method described in Japanese Patent Application No. A, the present invention provides a greater flexibility in that cane or cane meal can be stored or transported anywhere, and the reproduction method is independent of the cultivated land, and It can be done in a simple container that can be stored anywhere. The method of the present invention also reduces the risk of unbranched plants in the field, otherwise valuable farmland will be wasted. Since the sprout/seedling is protected during the first growth phase of sensitivity and only moves after its root system is well developed: to the field or exposed to the surrounding environment, the method is healthy and robust and grows from it. Adult sugar cane plants, especially when performing at least one of the above-mentioned aspects. Especially in the steps (χ) and / or (four), body. The step (in the case of a call, in particular, the steps include, by at least one fungicide and/or at least one insecticide, preferably by at least a seed fungicide and a special agent By treating with 100 grams of sensitization, a particularly healthy and sturdy seedling and an adult cultivar grown from these seedlings J63649.doc 201247102 cane plants are obtained. Example 1 Heat on the surface of 0.05 hectares is removed by machine cutting Approx.:5: The planting of sweet and sour plants is 'borrowing the top. Discard and remove... The lower part of the 8th-month-old Ganlian plant in the south is removed from the field, cut above the surface, and the rest contains - a section Block. Abandon =: The stalk is cut into about 5 to 10 (10) and the block is placed in a wet medium (sand bed) t, : 2::::: one: Add and set the stalk. _ Keep it, sprout (8) generation) has been humidified from the bud body 1 ^. In the 30th day of the bud (2nd generation), the top of the 4 new shoots is cut off from the top of the cut. After 30 days of the new top, it is pulled out by 3^. In the excision of the first generation of sprouts, each section of the Λ=: ί ^ dimension / warp stone wet. The second generation of the second generation, the 'maintained coconut fiber 镞f Μ 4, was placed in a separate pot filled with wet coconut fibers. Place the pots in the greenhouse

7. To the gift. Under the humidity, when the newly planted buds grow young C, the sprouts/seedlings are fully irrigated. After being placed in the greenhouse for 3 to 45 days, the surviving seedlings were transferred to the soil-to-soil adaptation zone and maintained. Thereafter, the number is counted and subsequently transplanted to the field. 96, _ seedlings have been obtained (average mother plants are recommended to produce 2 to 24 seedlings). This number is sufficient to plant on the 6.4 hectare area at the same row spacing, and the plants in each row are the same as in the field of origin. I63649.doc

52· S

Claims (1)

201247102 VII. Patent application scope: 1 · A method for cultivating sugar cane, the method comprising: (1) cutting a sugarcane plant from 6 to 18 months old into a block; (8) I requiring at least one fungicide and/or at least one An insecticide and/or at least one wound protection material and/or at least one growth regulator treating the cut surface of the sugar cane pieces obtained in step (1); (iii) being obtained in step (1) or (ii) The cane pieces containing at least one knot are placed in the wet medium; (iv) cut off the top of the meristem above the sprouts sprouted from the knots of the cane bars; (V) at least one as needed Treating the cut surface of the sprout obtained in step (i V) with a fungicide and/or at least one insecticide and/or at least one growth regulator; (Vi) resecting as needed from step (iv) or (v) The top of the newly formed sprout in which the sprout is germinated is located at the top of the meristem; (vii) treated with at least one fungicide and/or at least one killing agent and/or at least one growth regulator as needed In step (vi (viii) repeat the steps (Vi) and the steps (vii) as needed, one or several times as needed; (ix) from steps (iv), (V), (Vi) ), (vii) or (viiim cut new sprouts of new shoots grow to 10 to 6 cm long, cut them to include at least part of the meristem; 163649.doc 201247102 (X) It is desirable to treat the sprout obtained in step (ix) by at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one growth regulator; (xi) step ( The buds obtained in ix) or (X) are planted in a growth medium; (XII), as needed, by at least one fertilizer and/or at least one fungicide and/or shortly before, during or after planting. Treating the sprouts and/or growth medium with at least one insecticide and/or at least one nematicide and/or at least one growth regulator and/or at least one supersorbent and/or at least one growth promoting bacteria; (xiii) The sprout grows at a temperature of at least 15 ° C (xiv) by means of at least one fungicide and / or at least one insecticide and / or at least one nematicide and / or at least one growth regulator and / or at least one hair root agent and / or promote The growing bacteria treats the seedlings during growth and/or their growth medium; (xv) 10 to 12 days after the new shoots are planted in the growth medium, if the growth medium is not the field, the seedlings obtained from the sprouts are planted to the field Where the field has been at least one fertilizer and/or at least one fungicide and/or at least one insecticide and/or at least one nematicide and/or at least one growth regulator and/or / or at least one super sorbent and / or growth-promoting bacteria treatment, or if the growth medium is field - 'the seedlings obtained from the sprouts are exposed to the surrounding 163649.doc S 201247102 environment; and (χνι) as needed in the planting By at least one fertilizer and/or at least one fungicide and/or at least one insecticide during or after the field or after exposure to the surrounding environment / Or at least one other nematocides and / or at least one growth regulating agent and / or at least - kind of super adsorbent and / or seedlings and / or in the field of promoting bacterial growth and / or at least one polymer preservation process. 2. The method of claim 1, wherein in step (1), the live sugar cane plant of 6 to 18 months old is removed from its growth place before the string is cut into pieces. 3. The method of claim 2, wherein after the sugarcane plant is removed from the place where it is grown, the top of the cane bar is cut so that the lower cane bar still contains 5 to 15 knots. The method of any one of claims 2 or 3, wherein the sugar cane plant or the lower sugar cane thereof is removed by breaking or cutting over the soil. 5. The method of any of the claims, wherein in step (1), there will be; one section, preferably 1 to 3 sections, more preferably 1 or 2 sections, in particular, 1 section The lower part of the cane is cut into pieces. 6. The method of any of claims __, wherein the wet medium used in step (10) is selected from the group consisting of soil, clay, nutrient solution, vermiculite, coconut fiber, wet cellulosic material, hydroponic system, The gas tillage system, the nutrient membrane technology system, and the irrigation; gt system, and preferably are selected from the group consisting of insect to stone and scorpion fiber. A method according to any one of the preceding claims, wherein the cane pieces are placed in the wet medium such that the buds are laterally aligned with respect to the axis of gravity. 163649.doc 201247102 8. 9. 10. 11. 12. 13. 14. 15. The method of any of the preceding claims, wherein step (vi) is carried out. The method of any of the new IT claims, wherein in the step (ίχ), when the buds of the jiang dynasty grow to 2 〇 to 4 〇 (10)', preferably 25 to 3 〇 (10), they are removed. A method of arranging any of the claims, wherein in step (10), the shoot obtained in step or (8) is planted in a container containing growth medium. The method of claim 1, wherein the container is placed in a greenhouse, and in step (X111), the sprouts are allowed to grow in the greenhouse. The method of any of the items of the present invention, wherein in step (xi), the new shoot obtained in step (IX) or (x) is planted in a growth medium and allowed to grow under the cover sheet. . The method of any of the months of the month, in the step (10), allows the sprouts to grow at temperatures between 18 and 35. (:, preferably 22 to hunger and specifically 25 to 35 C) The above-mentioned method of any of the above-mentioned items, in the step (10), allows the sprouts to be 40 to 100%, preferably "to %. /. And better seedlings grow in humidity from 7〇 to 9〇%. The method according to any one of the preceding claims, wherein in step (xiv), by the y-type vegetalin fungicide and/or at least one GABA antagonist: N1 insecticide and optionally By at least one different fungicide and/or at least one different insecticide and/or at least one nematicide and/or at least one growth regulator and/or at least one hair root and/or growth promoting bacteria Treatment of these growing seedlings and/or their growth culture I63649.doc 201247102. 16. The method of claim 15, wherein the at least one ergonomics fungicide is selected from the group consisting of azoxystrobin, & dimoxystrobin, and enestr〇burin , fluoxastrobin, kresoxim_methyl, phenoxystrobin, oressastrobin, picoxystrobin, pyracl〇 str〇bin Pyraoxystrobin, pyramet〇str〇bin, pyribencarb, trifloxystrobin, 2-(2-(6. (3-gas-2-) Methyl-phenoxy)-5-fluoro-pyrimidin-4-yloxy)-phenyl)_2-methoxyimino-N-indenyl-acetamide, 3-decyloxy-2- (2-(N-(4-decyloxy-phenyl)-cyclopropane-carboxyiminothiomethyl)-phenyl)-decyl acrylate, (2-gas-5-[ 1-(3) -Methylbenzyloxyiminoethyl]benzyl)carbamic acid oxime S 曰 and 2-(2-(3-(2,6-monophenyl))_ι_indolyl-allylamine Alkylmethyl)-phenyl)-2-nonyloxyimido-N-mercapto-acetamide. The method of claim 16, wherein the blanket is The method of claim 15 wherein the at least one GABA antagonist is selected from the group consisting of acetoprole, endosulfan, and fennelup. (vaniliprole), pyraflupr〇le, pyriprole, phenylpyrazole compound I63649.doc 201247102 Wherein 1 ^ is (:| to (: 4 · alkyl or (^ to (: 4-haloalkyl; or an agriculturally acceptable salt thereof; and the base η than the salivation compound of formula 111) Or an agriculturally acceptable salt thereof. • 19. 20. 21. 22. The method of claim 18 wherein the gabΑ antagonist is fipronil, such as the method of any one of claims 10 or 11, wherein the sprout is planted The container is biodegradable. The method of claim 20, wherein in the step (χν), the seedling is not removed from the container and planted into the field. The method of claim 20 or 21, wherein the biodegradable container comprises a biodegradable material based on biodegradable poly 163649.doc 201247102 polylactic acid, raw rubber, curtain fish, wood chips, natural Ester, starch, cellulose, cellulosic materials, paper, paperboard, pulp from cellulose, grass fibers, and the like. 23. The method of any of the preceding claims, wherein in step (XV), the seedling is planted to the field using an automated or semi-automated planting machine. The method according to any one of the preceding claims, wherein in step (xiii), the shoot is planted in the step (XV), in the step (XV), after 25 to 80 days, preferably 40 to 70 days after the long medium. The seedlings are planted in the field or the seedlings are exposed to the surrounding environment. The method of any one of the preceding claims, wherein the at least one fungicide is selected from the group consisting of A) azoles selected from the group consisting of azaconazoles and oxazolyl alcohols ( Bitertanol), bromuconazole 'cyproconazole, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, beta 0 sit (etaconazole), nitrile benzene. Sitting (fenbuconazole), fluquinconazole, flusiazole, powder flutriafol, hexaconazole, imibenconazole, ipconazole , leaf fungus. Metaconazole, myclobutanil, oxpoconazole, paclobutrazole, penconazole, propiconazole, prothioconazole, simeconazole ), tebuconazole 163649.doc 201247102 (tebuconazole), tetrafluoron. Tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole, 1-(4-chloro-phenyl)-2-([1,2 , 4] triazol-1-yl)-cycloheptanol, cyazofamid, imazalil, pefurazoate, prochloraz, triflumizol, Benomyl, carbendazim, fuberidazole, thiabendazole, ethaboxam, etridiazole, hymexazole And 2-(4-chloro-phenyl)-N-[4-(3,4-dimethoxy-phenyl)-isoxazole-5-yl]-2-prop-2-ynyloxy B-acetamide; B) philoxins, selected from the group consisting of: azoxystrobin, dimoxystrobin, enestrobine, fluorinated . Fluoxastrobin, kresoxim-methyl, metominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, sputum Pyraoxystrobin, pyrametostrobin, pyribencarb, trifloxystrobin, 2-(2-(6-(3-chloro-2-indolyl-phenoxy) -5-fluoro-pyrimidin-4-yloxy)-phenyl)-2-decyloxyimido-N-mercaptoacetamide, 3-decyloxy-2-(2-(N-) (4-Methoxy-phenyl)-cyclopropane-carboxyiminothiomethyl)-phenyl)-isopropyl decyl ester, (2-chloro-5-[l-(3-methylbenzyl) Oxyimido)-ethyl]benzyl 163649.doc S 201247102 yl) guanyl decanoate and 2-(2-(3-(2,6-di-phenyl)-1-indenyl) Allyl-aminooxyindenyl)-phenyl)-2-decyloxyimidomethyl-acetamide; C) Carboxylamamines selected from the group consisting of: Bendale (benalaxyl), Benalaxyl-M, benodanil, bixafen, boscalid, carb〇xin Yue 0 Cardiff Amides (fenfuram), acyl cyclic amine bacteria (fenhexamid), Fu Duoning (flutolanil), furametpyr (furarnetpyr), than square. Isoprozil, isotianil, kiralaxyl, mepr〇nii, metaaXyi, madal-Μ (mefen〇xam) )), ofurace, oxadixyl, oxycarboxin, penthi〇pyrad, sedaxane, teci〇ftaiam, match Thifluzamide, Uadinil, 2-amino 4-mercapto-single _5_-mercaptoaniline, 2-gas-N-(l,l,3-trimethyl-anthracene -4--4-yl)-nicotine decylamine, N_(3',4',5,-trifluorobiphenyl-2-yl)-3-difluoromethyl-1-methyl-1H- ° than saliva _4_ guanamine (fluxapyroxade), Ν_(4·_trifluoromethyl thiobiphenyl-2-yl)_3_difluoromethyl-1-indolyl-1Η-pyrazole _ 4_ decylamine, ν-(2-(1,3-didecyl-butylphenyl) 1,3 dimethyl-5.fluoro-1 Η-.biazole-4_formamide and hydrazine -(2_(1,3,3-trimethyl-butyl)-phenyl)-1,3-dimercapto-5-fluoro-1 Η比圭 4 曱, amine, darfuryl (djmeth〇m〇 Rph), fluocrin 163649.doc -9- 201247102 (flumorph), pyromorph, Humetover, flupicolide, fluopyram, zoxamide, N-(3-ethyl-3,5,5-tridecyl-cyclohexyl) -3-carboxamido-2-hydroxy-benzamide, carpropamid, dicyclomet, mandiproamid, oxytetracyclin, 矽托凡Silthiofam) and Ν·(6-methoxy-pyridin-3-yl)cyclopropanoic acid decylamine; D) a heterocyclic compound selected from the group consisting of: fluazinam, bifenfen (pyrifenox) ' 3-[5-(4-Gas-phenyl)-2,3-dimercapto-iso- 0 ° ° sit--3-yl]-β ratio bite, 3-[5-(4- Mercapto-phenyl)-2,3-dimethyl-iso. Ester. Sodium decyl-3-yl]-η ratio. Fixed, 2,3,5,6_ four gas-4 - 曱院石黄Kibi bite, 3,4,5-three gas ratio"-2,6-dicarbonitrile, N-(l-(5-bromo-3-a-oxaridin-2-yl)-ethyl) -2,4-digas-nicotine decylamine, hydrazine-[(5-bromo-3-chloro-pyridin-2-yl)-methyl)·2,4-dichloro·final amine, Bray Bupirimate, cyprodinil, diflumetorim, fenarimol, fulmi Rimzone), mepanipyrim, nitrapyrin, nuarimol, pyrimethanil, triforine, seed dressing π (fenpici〇nii) Fluodixonil, aidimorph, dodemorph, carbendazim acetate, fenprofene (fenpr〇Pim〇i*ph), thirteen morpholine (tridem〇rph), benzene Rustidine 163649.doc -10- S 201247102 (fenpropidin), fluoroimid, iprodione, chlozolinate, procymidone, vinclozolin, Where to kill (famoxadone), feeding. Fenamidone, flutianil, octhilinone, probenazole, 5-amino-2-isopropyl-3-oxo-4-pyrylene S-allyl ester of bis- 2,3-dihydropyrazole-1-thiocarbamate, acibenzolar-S-methyl, 0 azulbrom, enemy Anilazin, blasticidin-S, captafol, captan, chinomethionat, dazomet, debacarb, up to Dimlomezine, difenzoquat, wild swallow-mercaptosulfate, fenoxanil, Folpet, ox〇linic acid, piperalin , proquinazid, pyroquilon, quinoxyfen, triazoxide, tricyclazole, 2-butoxy_6_iodo-3-propyl Terpene-4-one, 5-gas-l-(4,6-dimethoxy-pyrimidin-2-yl)-2-mercapto-1H-benzimidazole, 5-gas-7-(4- Nonylpiperidin-1-yl)-6-(2,4,6-trifluorophenyl)-[1,2,4]triazolo[i,5a]pyrimidine and ethyl -6-octyl-[1,2,4]triazolo[l,5-a]pyrimidin-7-ylamine; E)carbamate's which are selected from the group consisting of: Chaner (ferbam), zinc 乃 浦 ( (manc 〇 b b ( 猛 猛 猛 猛 猛 猛 man 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 163 (propineb), thiram, zineb, ziram, benthi aval icarb, pyributicarb, diethofencarb, carbendazim Iprovalicarb), iodocarb, propamocarb, probucol-hydrochloride, prothiocarb 'valiphenal' and N-(1-(1-(4- Cyano·phenyl)ethanesulfonyl)-butan-2-yl)carbamic acid-(4-fluorophenyl); F) other active compounds, which are selected from the group consisting of: Compounds: guanidine, dodine, doxine free test, guazatine, guazatine_acetate, iminoctadine, dihydrooctylamine triacetate ( Iminoctadine-triac Etate), diin-octylamine-tris (albesilate); antibiotics: kasugamycin, kasugamycin-hydrochloride-hydrate 'gelmycin, paeontin ( Polyoxine), validamycin A; schutyl benzene derivative: binapacryl 'dinobuton, dinocap, meptyldinocap, nitrothal -isopropyl) 'Tesenazen, organometallic compound · fentin salt, such as fentin-acetate, fentin chloride or triphenyl hydroxide Fetin hydroxide; I63649.doc •12 3 201247102 Sulfur-containing heterocyclic compounds: dithianon, isoprothiolane; organic breaking compounds: edifenphos, fosetyl , fosetyl-aluminum, iprobenfos, squamous acid and its salts, pyrazophos, tolclofos-methyl; organic compounds · four different Chlorothalonil, dichlofluanid, two gases (dichlorophen), flusulfamide, hexachlorobenzene, pencycuron, pentachlorophenole and its salts, phthalide, quintozene, hydrazine Thiophanate-methyl, tolylfluanid, N-(4-chloro-2-nitro-phenyl)-N-ethyl-4-indolyl benzenesulfonamide; inorganic Active material: Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulphate, sulfur; other: biphenyl, bronopol, cyflufenamid, gas benzene Chlorhexine, cymoxanil, dicloran, tecnazene, diphenylamin, metrafenone, mildiomycin, jing Oxin-copper, prohexadione, spiroxamine, tolyfluanid, N-(cyclopropyl decyloxyimido-( 6-difluoro-methoxy-2,3·difluoro-benzene 163649.doc 13 201247102 base)-mercapto)-2-phenylacetamide, N,-(4-(4 - gas-3-trifluoromethyl_phenoxy)-2,5-dimethyl-phenyl)-N-ethyl.N-mercaptomethyl hydrazine, Ν'-(4-(4.fluoro) 3_Trifluoromethyl-phenoxy)_2,5-didecylphenyl)-fluorenyl-ethyl-Ν-mercaptomethylhydrazine, ν,-(2-mercapto-5-trifluoromethyl) 4-(3-methylsulfanyl-propoxy)-phenyl)-indole-ethylindenyl hydrazine, hydrazine-(5-difluoromethyl-2-indole-4-(3-tri) Mercaptoalkyl-propoxy)-benyl)-N-ethyl-N-methylindole, 2-{i-[2-(5-fluorenyl-3-trifluoromethyl-pyrazole)基 醯 醯 哌 哌 _ _ 4 4 4 4 4 4 4 - - - - - - - - - - - - - - - - - - - - 2- 2- 2- 2- 2- 、 、 、 、 、 、 、 、 、 -(5-mercapto-3-trifluoromethylpyrazole 4• ketoethyl)-piperidin-4-ylthiazole_4-carboxylic acid, methyltetrahydro-naphthalene-1-yl)-oxime Amine, acetic acid tert-butyl _8_ι_2, 3-dimethyl quinolin-4-yl ester and decyloxy-acetic acid 6 · tert-butyl _8 • fluoro-2,3-didecyl-quinoline -4-yl ester; and G) biological control agent. 26. The method of claim 25, wherein the biological control agent is selected from the group consisting of non-pathogenic bacteria, preferably selected from the group consisting of Pseud〇m〇nas fluorescens, Pseudoinonas putida ), Streptomyces griseus, Streptomyces ochraceisleroticus, Streptomyces graminofaciens, Streptomyces corchousii, Streptomyces spiroverticillatus, gray-green Streptomyces 163649.doc • 14· S 201247102 griseovirdis, Streptomyces hygroscopicus, Bacillus subtilis, Bacillus cereus, Bacillus mycoides, short buds Bacinus pumilus, Bacillus licheniformis, Bacillus thuringensis, and metabolites produced from the bacteria; the non-pathogenic fungus 'is preferably selected from Trichoderma spp. Trichoderma harzianum, Trichoderma virida e) Verticillium lecanii, Sporidesmium sclerotiorum and Zygomycetes, and metabolites produced from the fungus; resinic acid; Reynoutria sachalinensis The plant extract; and the plant defense inducer' is preferably a plant harpin. The method of any one of claims 25 or 26, wherein the at least one fungicide is selected from the group consisting of cyproconazole, difenoconazole 'epoxiconazole, and sputum (fluquinconazole), flusilazole, flurifaol, and leaf fungus. Sit (metconazole) 'nitrilase. Sit (myclobutanil), penconazole, propiconazole, and prothiotoxin. Sitting (pr〇thioconazole), triadimefon, triadimenol, tebuconazole, tetraconazole, triticonazole, prochl〇raz ), cyazofamid, benomyl, carbendazim 15 163649.doc 201247102 (carbendazim), ethaboxam, azoxystrobin, dim〇xyStrobin Fluorine "fluoxastrobin, fluorobenzene" bismuth (fiuxapyroxade), kresoxim-methyl, orysastrobin. Picoxystrobin, pyracyr〇str〇bin, trifloxystrobin, bixafen, b〇scalid, sedaxane, ring Fenhexamid, metalaxyl, 0 ratio. iSOpyrazam, mefenoxam, furosemide, dimethomorph, flumorph, fluopicolid (flurazia) Picobenzamid, zoxamide, carpr0pamid, mandipropamid, fluazinam, cyprodinil, fenarimol, Mepanipyrim 'pyrimethanil, triforine, fludioxonil, dodemorph, fenpropimorph, tridemorph, Fenpropidin, iprodione, vinclozolin, famoxadone, fenamidone, probenazole, proquinazid, S - acibenzolar-S-methyl, captafol, folpet, fenoxanil, quinoxyfen, 5-ethyl-6-octyl -[1,2,4]triazolo[l,5-a] benzophenanthidine, zinc, mancozeb, metiram, 163649.d Oc -16- 3 201247102 Propineb, thiram, iprovalicarb, flubenthiavalicarb (benthiavalicarb), propamocarb , dithianon, fentin salt, fosetyl, fosetyl-aluminium, H3PO3 and its salts, chlorthalonil, Dichlofluanid, thiophanat-methyl, copper acetate, copper hydroxide, chlorinated steel 'stone copper, sulfur, cymoxanil, metrafenone, squirrel Spiroxamine and Bacillus subtilis and their metabolites. 28. The method of claim 27, wherein the at least one fungicide is selected from the group consisting of azoxystrobin, dimoxystrobin, fluoxastrobin, and flubenpyoxade ), kresoxim-methyl, ory Sastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin, and Bacillus subtilis and their metabolism And, preferably, pyraclostrobin. 29. The method of any one of the preceding claims, wherein the at least one insecticide is selected from the group consisting of a) a pyrethroid compound selected from the group consisting of acrinathrin, allethrin, d - cis-trans arylene (d-cis_trans_ allethrin), d-trans arylene (d_t; rans allethrin), bifenthrin, biocetoacetine (bi〇aiiethrin), bio-sweet Ester S-cyclopentyl, biopterin (bi〇resnlethrin), B 163649.doc 201247102 Cyprothrin (cycloprothrin), cyfluthrin, β-cyfluthrin, competition Cyhalothrin, λ-cyhalothrin, γ-cyhalothrin, cypermethrin, α-cypermethrin, β-赛灭β (β-cypermethrin), θ-cypermethrin, ξ-cypermethrin, cyphenothrin, deltamethrin, methicillin Empenthrin), esfenvalerate, etfenfenx, fenpropathrin, fenvalerate, Flucythrinate, flumethrin, τ-fluvalinate, halfenprox, imiprothrin, metofluthrin, hundred Permethrin, phen〇thrin, prallethrin, profiuthrin, pyrethrin (pyrethrum), benzfur Resmethrin, siiafiuofen, tefluthrin, tetramethrin, tralmethrin and transfiuthrin; b) nicotinic acid a agonist/antagonist compound selected from the group consisting of acetamiprid, bensultap, cartap hydrochloride, clothianidin, dinotefuran, Yida Amine (imidacloprid), thiamethoxam, nitenpyram, nicotine, spinosad (ectopic agonist), spinosyn 163649.doc •18-S 201247102 (spinetoram) (ectopic agonist), thiacloprid ' insecticidal ring (thiocyc Lam), thiosultap-sodium and AKD 1022; c) GABA gated airway antagonist compound selected from the group consisting of chlordane, endosulfan, gamma-HCH (lingali) Lindane)), ethiprole, fipronil, pyrafluprole and pyriprol; d) chloride channel activator selected from abamectin , emamectin benzoate, milbemectin and lepimectin; and e) chitin biosynthesis inhibitor: (el) benzoquinone: double worm Bistrifluron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron 'bis benzene fluoride Urea (novaluron), polyfluorogland (novifiuniuron), teflubenzuron, triflumuron. 30. The method of claim 29, wherein the at least one insecticide is selected from the group consisting of fipronil, acetamiprid, chlothianidin, imidacloprid, Sai Su'an. The method of any one of the preceding claims, wherein the at least one nematicidal agent is selected from the group consisting of: 163649.doc 19 201247102 Antibiotic nematicidal (Aphis sinensis) Agents such as abamectin; plant nematicides such as carvacrol; Quillaja or Gleditsia extract; saponines; urethane nematicides, Selected from benomyl, carbofuran, carb〇Sulfan and cloethocarb; 躬^-aminoformate nematicidal agent' selected from cotton ringworm (alanycarb) ), aldicarb, aldoxycarb, oxamyl, and tirpate; a fumigant nematicide selected from the group consisting of dimethyl disulfide (dithioether) Mercapto bromide; organophosphate nematicide: organic acid salt killing Agent, selected from the group consisting of diamidafos; fenamiphos; fosthietan and phosphamidon; organothiophosphate nematicides selected from cadusafos , chlorpyrifos, dichlofenthion, dimethoate, ethoprophos, fensulfothion, fosthiazate, heterophos, and stagnation Isamidofos), isazofos, phorate, phosphocarb 'terbufos', terionazin and trispin 163649.doc • 20· S 201247102 (triazophos); a thiophosphonite nematicide selected from the group consisting of imicyafos and mecarphon; and an unclassified nematicide selected from the group consisting of acetoprole and isothiaclones. Benclothiaz), chloropicrin, dazomet, DBCP, DCIP, 1,2-dichloropropane, 1,3-dipropene, flubensulfonite < (fluensulfone), brewing ( Furfural), metam, methyl iodide, methyl isothio The method of any one of the preceding claims, wherein the growth promoting bacteria are selected from the group consisting of Azospirillum, Azotobacter, Nitrogen. Azomonas, bacillus, beijerinckia, burkholderia, cl〇stridium, cyanobacteria Cyanobacteria), enterobacter, erwinia, gluconobacter, klebsiella, and streptomyces bacteria. 3. The method of claim 32 wherein the growth promoting bacteria are selected from the group consisting of Azospirillum amazonense, Herbaspirillum seropedicae, and Herbaspirillum rubrisubalbicans. ), Burkholderia tropica, Gluconacetobacter diazotrophicus, Pseudomanas fluorescens, Pseudomonas putida (Pseud〇m〇nas 163649.doc 21 201247102) Putida), Streptomyces griseus, Streptomyces ochraceisleroticus, Streptomyces graminofaciens, Streptomyces corchousii, Streptomyces spiroverticillatus, Phytophthora nicotiana (Streptomyces griseovirdis), Streptomyces hygroscopicus, Bacillus subtilis 'Bacillus cereus, Bacillus mycoides, Baciiius pumiius' lichens Bacillus liche The method of any one of the preceding claims, wherein the at least one growth regulator is selected from the group consisting of nonylcyclohexanedione, mepiquat chloride And chlorinequat chloride, preferably selected from the group consisting of prohexadione, prohexadi〇ne_Ca, trinexapac, trinexapac_ethyi, and strong cotton Mepiquat chloride and chlormequatchl〇ride. The method of any one of the preceding claims, wherein the at least one hair root agent is selected from the group consisting of a philophilic fungicide, a nicotinic acid receptor agonist/antagonist auxin, gibberellin (gibberellins), gibberellic acid, cytokinin, humic add, quince (Quillaja) or Gleditsia extract, saP〇nines, biological control agents and Plant defense inducer. The method of any of the preceding claims, wherein the wound protection material is 163649.doc • 22· S 201247102 is selected from the group consisting of non-read inorganic and organic film formation or 37. The method of claim 36, wherein the wound Guarantee: The combination and sealing materials are selected from super adsorbent, super-adsorbent treated polymer, aliphatic-aromatic..., fresh-keeping polymer, wax, soluble glass and Tingui seed coat preparation. 0 163649.doc - 23· 201247102 IV. Designated representative map: (1) The representative representative of the case is: (none) (2) The symbolic symbol of the representative figure is simple: 5. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention. : (none) 163649.doc