201217775 六、發明說明： 【發明所屬之技術領威】 [0001]本發明係有關一耧檢測試片，特別是關於一種電化學檢 測試片。 [先前技術3 [0002] 由於機電技術的發展’在檢測生物檢體時，大多使用電 化學式或是光學式的反應加以偵測。利用前者（電化學 )反應的情況’舉例而言，在偵測血糖時，係以血液樣 本中的葡萄糖與測試試片上搭載的葡萄糖氧化臃（gluc- Π ose oxidase，GOD)進行氣化還原反應，藉由氧化還原 反應中所產生的電子或電流等訊號，以禎測參與反應的 葡萄糖含量’藉此換算成血糖濃度。而利用'後耆（光學 )反應的情況，則可以葡萄糖與酵素反應缘所造成測試 試片的顏色變化，再藉由比色法將顏色變也量換算成血 糖濃度。 - ：：:· . - t [0003] 而目前採用電化學反應的檢測試片，由於需要偵測反應 〇 中所產生的電子電流等訊號，因此需要有導電電極接收 這些訊號’並且傳導至測量儀器中進行換算。習知技術 的導電電極有在銅質電極鍍上鎳及鈀，或是將銀膠印刷 後塗上活性碳層以製成電極。由於銅質電極在製作上多 利用電鐘等方式，將後續的鎳與鈀鍍在銅金屬層上，製 作成本較高。此外，亦有利用蒸鍍或濺鍍等方式，將惰 性金屬（金、鉑、鈀）直接鍍在基板上’後續再使用蝕 刻製程將多餘部份去除，僅留下需要的電路部份，這種 製程則會造成更多的材料耗損，且更提昇製造成本。但 099135366 表單編號A0101 第3頁/共16頁 0992061785-0 201217775 是使用在印刷好的銀膠線路上塗佈有活性碳層以製成電 極的方式，雖然成本較前述採用電鍍或蒸鍍濺鍍等方式 為低，但是所製成的檢測試片，較前述採用惰性金屬為 電極外層的檢測試片，在測量時的精準度與穩定度不佳 ，反而使得品管（QC)上要耗費額外的成本。 【發明内容】 [0004] 為克服上述缺點，本發明提供一種電化學檢測試片，包 含有基板以及設置於基板上的導電電極。導電電極則包 含有依序堆疊的導電膠體層、第一金屬層，第二金屬層 ，以及第三金屬層。導電膠體可為銅膠、鎳膠、銀膠、 銀碳膠、或是碳膠；第一金屬層則是由第VI11族金屬組 成；第二金屬層則由鎳組成；而第三金屬層是鈀（Pd) 、金（Au)或是始（Pt)金屬所組成。 [0005] 因此，本發明之主要目的係提供一種電化學檢測試片 ，由於採用導電膠體，因此易於製造，可有效降低成本 〇 [0006] 本發明之另一目的係提供一種電化學檢測試片，由於 係以鈀、金或是鉑金屬形成電極外層，因此可有效提昇 測量的敏感性以及特異性。 [0007] 此外，本發明亦提供另一種電化學檢測試片，包含有基 板以及設置於基板上的導電電極。導電電極則包含有依 序堆疊的導電膠體層、第一金屬層，第二金屬層，以及 第三金屬層。導電膠體可為銅膠、鎳膠、銀膠、銀碳膠 、或是碳膠，且導電膠體層係經由印刷流程印刷於基板 上。第一金屬屠則是由第V111族金屬組成，並且是經將 099135366 表單編號A0101 第4頁/共16頁 0992061785-0 201217775 印刷有導電膠體層的基板經過電漿清洗與酸洗程序後， 再經過化學鍍方式鍍於導電膠體層上。第二金屬層則由 鎳組成，且經化學鍍方式鍍於第一金屬層上。而第三金 屬層是鈀、金或是鉑金屬所組成，且經化學鍍方式鍍於 第二金屬層上。 [0008] Ο [0009] [0010] ο [0011] 099135366 本發明之另一目的係提供一種電化學檢測試片，由於採 用印刷方式將導電膠體印刷於基板上，因此易於製造， 可有效降低成本。 本發明之另一目的係提供一種電化學檢測試片，由於係 以鈀、金或是鉑金屬形成電極外層，因此可有效提昇測 量的敏感性以及特異性。 【實施方式】 由於本發明係揭露一種電化學檢測試片，其中所利用電 化學原理及電路印刷技術，已為相關技術領域具有通常 知識者所能明瞭，故以下文中之說明，不再作完整描述 。同時，以下文中所對照之圖式，係表達與本發明特徵 有關之示意，並未亦不需要依據實際情形完整繪製，合 先敘明。 首先，請參考第1Α圖，為本發明第一較佳實施例電化學 檢測試片示意圖。電化學檢測試片1包含有基板11以及 鋪設於基板11之上的導電電極12，在導電電極12上蓋設 有絕緣層13。其中基板11的材質可以是聚乙烯對苯二曱 酸酯（PET)、聚碳酸酯（PC)等生物惰性塑膠（bioinert plastic) 材料 ，亦可採用聚 亞醯胺 （poly im-ide)、玻璃纖維板、或是電木板（酚醛樹脂）。 表單編號A0101 第5頁/共16頁 0992061785-0 201217775 [0012]請參考第1 B圖’為第i A圖中電化學檢測試片1沿A A連線 的剖面示意圖。導電電極12的構成，如同圖中所示，由 基板11向上’依序堆疊有導電膠體層12〇、第一金屬層 121、第二金屬層122、以及第三金屬層123。而導電膠 體層120則為含有金屬導體或碳粒子的樹脂膠體，其材質 可以是銅膠（copper paste)、鎳膠（nickel paste )、銀膠（silver paste)、銀碳膠（含有銀粒子與碳 粒子的樹脂膠）、或是碳膠，並且採用印刷方式將導電 膠體層120印刷於基板11之上。第二金屬層122的材質則 採用鎳。此外’在印刷導電膠體層12〇之後，會將印有導 電膠體層120的基板1〇，經過清洗程序，利用電漿（ Plasma)去除導電膠體的雜質，並且再藉由酸洗程序將 導電膠體層120的表面活化，以利後續第一金屬層121、 第二金屬層122以及第三金屬層123的製作。 [0013] 另外，由於導電膠體層120之中的導電材料，如上所述， j ： , ： . ：'. 是使用銅、鎳、銀、碳，或是銀碳膠，因此為了要將第 二金屬層122的鎳以及第三金屬層123採用化學鍍的方式 堆疊上去，第一金屬層121的材質則是要使用第VIII族金 屬’亦即要使用鎳（nickel ’ Ni )、鈀（pai iadium， Pd)、或是鉑（Platinum ’ Pt)金屬；其中又以使用把 的情況，對於後續第二金屬層122與第三金屬層123的化 學鑛效果較好。此外’導電膠體層12〇中所使用的膠體樹 脂的材質則與印刷上去的基板材質為相同時（例如基板 11和導電膠體層120中膠體樹脂的材質均是聚乙稀對笨二 甲酸醋），對於後續進行第一金屬層121的化學錢的效果 099135366 表單編號A0101 第6頁/共16頁 0992061785-0 201217775 較好。此外’導電膠趙層 屬層121的化學鍍的結果 120的印刷厚度也會_$_| [0014] Ο 為了要能讓電化學Μ巾所纽的電句 電電極12，以利後續的訊號測量以及相對—到導 濃度換算，第三金屬層123的材質是使用絶、=物質 具有良好導電性的金屬，其中又以使_ ，或者麵等 學檢測試Μ在使㈣量的精準度為最佳成的電化 當作觸媒，㈣於仏學反應喊生。雖本身可以 果最佳’但因翻的材料成本較高，因此，=始的效 試片測量精準度兩輕平衡考量下，也可叫=本與 第三金屬層123的材質。 金做為 [0015] ο [0016] 較佳實施财所示的，在整條導 電電極12中都锻有第-金屬㈣、第二金屬層122以及 第三金屬層123的情況（亦即是鍵在整個導電膠體層120 的上面）’也可以依照實際情況，只在導電電極12的部 份區域鍍上第-金屬層121、第二金屬層122與第三金屬 層 123。 因此’本發明的第二較佳實施例，亦為_種電化學檢測 試片。請參考第2A圖，電化學檢測試片2與第一較佳實施 例中所述的電化學檢測試片丨大致相同，亦包含有基板21 以及鋪設於基板21之上的導電電極22 ,而在導電電極22 上蓋設有絕緣層23。與第一較佳實施例不同之處是電化 學檢測試片2的導電電極22，進一步包含有反應區22a、 插入區22c，以及介於反應區22a與插入區22c之間的傳 導區22b。反應區22a上則塗佈有反應材料24，以供與待 099135366 表單編號A0101 第7頁/共16頁 0992061785-0 201217775 測物質（未圖示）進行f化學反應，藉此產生電子電流 等訊號·，而在反應區22a所產㈣電子電流峨，是經由 傳導區22b傳導至插422e。插人區❿則是電化學反 應試片2與測量儀器（未圖示）連接的區域。在實際使用 時，插入區22c會藉由與測量儀器（未圖示）的連接，將 電子電流訊號傳送至測量儀器中進行運算，藉此換算成 待測物質的濃度等資訊。 [0017] [0018] [0019] 請再參考第2B圖，為第_中所示的電化學檢測試片2沿 BB連線的剖面示意圖。如圖中所示，在基板以上則是 自反應區22a、傳導區22b—直到連接區22c都有印刷有 導电膠體層220。而在連接區'2 2c處，在導電膠體層220 之上直接蓋设有絕緣層23。而在..反應.區.22a處，則是自導 电膠體層220起依序堆疊有第一金屬層221、第二金屬層 222以及第三金屬層223。 此外，可根據實際需求’將依序堆疊的第一金屬層221、 第二金屬層222以及第三金屬層223設置於連接區22c處 ;或是更進一步地將第一金屬層221、第二金屬層222以 及第三金屬層223設置在反應區22a與連接區22c兩處。 上述設計即是在電化學反應試片2中，並非如同第一較佳 實施例中是沿著整條導電膠體層220都鍍有第—金屬層 221、第二金屬層222以及第三金屬層223。由於反應區 22a是塗佈有反應材料24，待測物質（未圖示）與反應材 料2 4進行電化學反應後所產生的電子或電流，需要傳導 至反應區22a的導電電極22上，因此此處需要有良好導電 性的導體金屬，藉此降低阻抗以及導電電極22的訊嗓比 099135366 表單編號A0101 第8頁/共16頁 0992061785-0 201217775 [0020] Ο [0021] Ο [0022] (Signal/Noise Ratio)，以増進電化學反應試片2在 測量上的敏感性（sensitivity)以及特異性（賢 C1f1C1ty)。此外，由於連接區22c的導電電極22是需 要將電子或電料城傳送朗量_巾進行運算，因 此此處使料有1好導雜的導體金·，亦有助於增進 電化學反應試片2在測量上的敏感性以及特異性。 因此’在製作時’可以在基板21印刷上導電膠禮層22〇， 經過«清洗以及酸洗程序後，在傳導區22b處的導電膠 體層220噴上或塗上一層絕緣漆22〇1。藉此’讓傳導區 22b處的導電膠體層220在後績的化學鍍製程中，不會再 與化學鍍所使用的反應溶液接觸，使得傳導區22b處的導 電膠體層220不再鍍上金屬層，可以有效節省第一金屬層 221、第一金屬層222與第三金屬層223中所需的惰性金 屬（例如金、鉑、鈀）用量，以降低製造成本。 此外’第二較佳實施例中所述的基板21、導電膠艘層220 、第一金屬層221、第二金屬層222以芩第三金屬層223 ’其材質以及較隹的配置狀態則與第一較佳實施例中所 述大致相同，在此不再重複贅述。 本發明進一步提供第三較佳實施例，其結構與第一較佳 實施例相同，故請參考第1A圖與第1B圖。本發明中的第 三較佳實施例所提供的電化學檢測試片11包含有基板1 1 以及铺設於基板11之上的導電電極12，在導電電極12上 蓋設有絕緣層13。而導電電極12的構成，則由基板η向 上’依序堆疊有導電膠體層120、第一金屬層12ι、第二 金屬層122、以及第三金屬層123。 099135366 表單編號A0101 第9頁/共16頁 0992061785-0 201217775 [0023] 導電膠體層120可為銅膠、鎳膠、銀膠、銀碳膠’或是碳 膠所組成且導電膠體層12Q係經的刷流程印刷於基板 11上。第一金屬層121則是由第VIII族金屬組成，並且 是經將印刷有導電膠體層120的基板11經過電漿清洗與酸 洗程序後，再經過化學鍍方式鑛於導電膠體層12〇上。第 一金屬層122則由鎳組成。而第三金屬層123是鈀 '金或 是翻金屬所組成。而第二金屬層m與第三金屬層123同 樣地是經過化學鍍方式分別鍍於在第—金屬層12丨與第二 金屬層122之上。此外，第一金屬層121則是將印刷有導 電膠體層120且經過電漿清洗與酸洗程序的基板n，浸泡 到含有第VIII族金屬離子的電解液中，控制電解的溫度 、時間以及電解液中的第VI Π族金屬的離子濃度以及電 解液的pH值在適當範圍，將第一金屬層121鍍在導電膠體 層120上。而第二金屬層122與第三金屬層123的化學鍍 方式，則與第一金屬層121的化學鍍方式類似，只是在將 第二金屬層122鍍到第一金屬層121之上時，是將已經鑛 上第一金屬層121的基板II浸泡糾含者録離子的電解液中 ，而第三金屬層123鍍在第二金屬層122上時則將已經鍍 上第一金屬層122的基板11浸泡到含有是把、金或是始離 子的電解液中’並且控制電解的溫度、時間以及電解液 中要鍍上去的金屬離子濃度以及電解液的pH值在適當範 圍。 以上所述僅為本發明之較佳實施例，並非用以限定本發 明之申請專利權利；同時以上的描述，對於熟知本技術 領域之專門人士應可明瞭及實施，因此其他未脫離本發 099135366 表單蝙號A0101 第10頁/共16頁 0992061785- [0024] 201217775 明所揭示之精神下所完成的等效改變或修飾，均應包含 在申請專利範圍中。 【圖式簡單說明】 [0025] 第1 A圖，為本發明第一較佳實施例電化學檢測試片示意 圖。 .201217775 VI. Description of the Invention: [Technical Leadership of the Invention] [0001] The present invention relates to a test strip for testing, and more particularly to an electrochemical test strip. [Prior Art 3 [0002] Due to the development of electromechanical technology, most of the biochemical samples are detected using electro-chemical or optical reactions. In the case of the former (electrochemical) reaction, for example, when detecting blood sugar, the glucose in the blood sample is subjected to gasification reduction reaction with gluc- ose oxidase (GOD) carried on the test piece. The glucose content involved in the reaction is measured by a signal such as an electron or a current generated in the redox reaction, thereby converting the blood glucose concentration into a blood glucose concentration. In the case of the 'post-twist (optical) reaction, the color change of the test piece can be caused by the reaction between glucose and the enzyme, and the color change amount is converted into the blood sugar concentration by the colorimetric method. - :::· . - t [0003] At present, the detection test piece using electrochemical reaction requires a conductive electrode to receive these signals' and conducts to the measurement because of the need to detect signals such as electron current generated in the reaction enthalpy. Conversion is performed in the instrument. The conductive electrode of the prior art has a nickel electrode and a palladium plated on the copper electrode, or a silver paste is printed and coated with an activated carbon layer to form an electrode. Since the copper electrode is fabricated by using an electric clock or the like, the subsequent nickel and palladium are plated on the copper metal layer, and the manufacturing cost is high. In addition, the inert metal (gold, platinum, palladium) is directly plated on the substrate by means of evaporation or sputtering. Then the etching process is used to remove the excess portion, leaving only the required circuit portion. This process will result in more material wear and increase manufacturing costs. However, 099135366 Form No. A0101 Page 3 of 16 0992061785-0 201217775 is a method of coating an activated carbon layer on a printed silver glue line to form an electrode, although the cost is higher than the foregoing using electroplating or vapor deposition sputtering. The method is low, but the test piece produced is lower than the above-mentioned test piece using the inert metal as the outer layer of the electrode, and the accuracy and stability of the measurement are not good, but the quality of the quality control (QC) is extra. the cost of. SUMMARY OF THE INVENTION [0004] In order to overcome the above disadvantages, the present invention provides an electrochemical test strip comprising a substrate and a conductive electrode disposed on the substrate. The conductive electrode includes a conductive colloid layer, a first metal layer, a second metal layer, and a third metal layer which are sequentially stacked. The conductive colloid may be copper glue, nickel glue, silver glue, silver carbon glue or carbon glue; the first metal layer is composed of the metal of group VI11; the second metal layer is composed of nickel; and the third metal layer is Palladium (Pd), gold (Au) or initial (Pt) metal. [0005] Therefore, the main object of the present invention is to provide an electrochemical test strip which is easy to manufacture and which can effectively reduce the cost by using a conductive colloid. [0006] Another object of the present invention is to provide an electrochemical test strip. Since the electrode outer layer is formed of palladium, gold or platinum metal, the sensitivity and specificity of the measurement can be effectively improved. In addition, the present invention also provides another electrochemical test strip comprising a substrate and a conductive electrode disposed on the substrate. The conductive electrode includes a conductive colloid layer stacked in sequence, a first metal layer, a second metal layer, and a third metal layer. The conductive paste may be copper glue, nickel glue, silver glue, silver carbon glue or carbon glue, and the conductive glue layer is printed on the substrate through a printing process. The first metal butcher is composed of the metal of Group V111, and after the plasma cleaning and pickling process is performed on the substrate printed with the conductive colloid layer of 099135366 Form No. A0101 Page 4 / 16 Page 0992061785-0 201217775 Electroless plating is applied to the conductive colloid layer. The second metal layer is composed of nickel and is electrolessly plated on the first metal layer. The third metal layer is composed of palladium, gold or platinum metal and is electrolessly plated on the second metal layer. [0009] [0010] 099135366 Another object of the present invention is to provide an electrochemical test strip, which is easy to manufacture and can effectively reduce cost by printing a conductive paste on a substrate by printing. . Another object of the present invention is to provide an electrochemical test strip which is effective in improving the sensitivity and specificity of the measurement since the electrode outer layer is formed of palladium, gold or platinum metal. [Embodiment] Since the present invention discloses an electrochemical detection test piece, the electrochemical principle and the circuit printing technology utilized by the present invention have been known to those skilled in the relevant art, and therefore, the description below will not be completed. description. At the same time, the drawings referred to in the following texts are indicative of the features relating to the features of the present invention and are not required to be completely drawn according to the actual situation, which will be described in the prior. First, please refer to FIG. 1 for a schematic diagram of an electrochemical detection test piece according to a first preferred embodiment of the present invention. The electrochemical test strip 1 includes a substrate 11 and a conductive electrode 12 laid on the substrate 11, and an insulating layer 13 is covered on the conductive electrode 12. The material of the substrate 11 may be a bioinert plastic material such as polyethylene terephthalate (PET) or polycarbonate (PC), or poly im-ide or glass. Fiberboard, or bakelite (phenolic resin). Form No. A0101 Page 5 of 16 0992061785-0 201217775 [0012] Please refer to FIG. 1B' for a cross-sectional view of the electrochemical test strip 1 along line A A in the figure iA. The conductive electrode 12 is constructed such that a conductive colloid layer 12, a first metal layer 121, a second metal layer 122, and a third metal layer 123 are sequentially stacked upwardly from the substrate 11 as shown in the drawing. The conductive colloid layer 120 is a resin colloid containing a metal conductor or carbon particles, and the material thereof may be a copper paste, a nickel paste, a silver paste or a silver carbon paste (containing silver particles and A carbon paste of a carbon particle, or a carbon paste, and the conductive paste layer 120 is printed on the substrate 11 by printing. The material of the second metal layer 122 is made of nickel. In addition, after the conductive paste layer 12 is printed, the substrate on which the conductive colloid layer 120 is printed is rubbed, the impurities of the conductive colloid are removed by plasma, and the conductive colloid is removed by a pickling process. The surface of layer 120 is activated to facilitate subsequent fabrication of first metal layer 121, second metal layer 122, and third metal layer 123. [0013] In addition, since the conductive material in the conductive colloid layer 120, as described above, j : , : . : '. is using copper, nickel, silver, carbon, or silver carbon glue, so in order to be the second The nickel of the metal layer 122 and the third metal layer 123 are stacked by electroless plating, and the material of the first metal layer 121 is to use a metal of the group VIII, that is, nickel (Ni), palladium (pai iadium) is used. , Pd), or platinum (Platinum 'Pt) metal; wherein the use of the handle, for the subsequent second metal layer 122 and the third metal layer 123 chemical ore effect is better. In addition, the material of the colloidal resin used in the conductive adhesive layer 12 is the same as the material of the printed substrate (for example, the material of the colloidal resin in the substrate 11 and the conductive colloid layer 120 is polyethylene terephthalic acid vinegar) For the subsequent effect of the chemical money of the first metal layer 121 099135366 Form No. A0101 Page 6 / Total 16 pages 0992061785-0 201217775 Preferably. In addition, the printed thickness of the result of the electroless plating of the conductive adhesive layer 121 is also _$_| [0014] Ο In order to enable the electro-electrode electric electrode 12 of the electrochemical wipe to facilitate the subsequent signals The measurement and the relative-to-conductivity conversion, the material of the third metal layer 123 is a metal having good electrical conductivity, and the accuracy of the (four) amount is determined by using _ or the surface test. The best electricity is used as a catalyst, and (4) it is shouted in the dropout response. Although it can be the best in itself, but because of the high cost of the material, the measurement accuracy of the original test piece can be called the material of the third metal layer 123 under the two light balance considerations. [0015] As shown in the preferred embodiment, the case where the first metal (four), the second metal layer 122, and the third metal layer 123 are forged in the entire conductive electrode 12 (that is, The key may be on the entire surface of the conductive paste layer 120. The first metal layer 121, the second metal layer 122 and the third metal layer 123 may be plated only in a partial region of the conductive electrode 12 as the case may be. Therefore, the second preferred embodiment of the present invention is also an electrochemical test strip. Referring to FIG. 2A, the electrochemical test strip 2 is substantially the same as the electrochemical test strip 所述 described in the first preferred embodiment, and includes a substrate 21 and a conductive electrode 22 laid on the substrate 21, and An insulating layer 23 is covered on the conductive electrode 22. The difference from the first preferred embodiment is that the conductive electrode 22 of the electrochemical test strip 2 further includes a reaction zone 22a, an insertion zone 22c, and a conduction zone 22b interposed between the reaction zone 22a and the insertion zone 22c. The reaction zone 22a is coated with a reaction material 24 for f-chemical reaction with a substance (not shown) of Form No. 099135366 Form No. A0101/16 pages 0992061785-0 201217775, thereby generating signals such as electronic currents. The electron current 产 (4) produced in the reaction zone 22a is conducted to the plug 422e via the conductive region 22b. The insertion area is an area where the electrochemical reaction test piece 2 is connected to a measuring instrument (not shown). In actual use, the insertion area 22c is connected to a measuring instrument (not shown) to transmit an electronic current signal to the measuring instrument for calculation, thereby converting the information into the concentration of the substance to be tested. [0019] Referring again to FIG. 2B, it is a schematic cross-sectional view of the electrochemical test strip 2 shown in the first section along the line BB. As shown in the figure, above the substrate, the self-reactive region 22a and the conductive region 22b are used until the connection region 22c is printed with the conductive colloid layer 220. At the connection region '2 2c, an insulating layer 23 is directly covered on the conductive paste layer 220. At the reaction area 22a, the first metal layer 221, the second metal layer 222, and the third metal layer 223 are sequentially stacked from the conductive colloid layer 220. In addition, the first metal layer 221, the second metal layer 222, and the third metal layer 223 which are sequentially stacked may be disposed at the connection region 22c according to actual needs; or the first metal layer 221 and the second layer may be further disposed. The metal layer 222 and the third metal layer 223 are disposed at two locations of the reaction zone 22a and the connection zone 22c. The above design is in the electrochemical reaction test strip 2, and unlike the first preferred embodiment, the first conductive layer 220 is plated with the first metal layer 221, the second metal layer 222, and the third metal layer. 223. Since the reaction zone 22a is coated with the reaction material 24, the electrons or current generated by the electrochemical reaction between the substance to be tested (not shown) and the reaction material 24 need to be conducted to the conductive electrode 22 of the reaction zone 22a. Here, a conductor metal having good conductivity is required, thereby reducing the impedance and the signal ratio of the conductive electrode 22. 099135366 Form No. A0101 Page 8 / Total 16 Page 0992061785-0 201217775 [0020] Ο [0021] Ο [0022] Signal/Noise Ratio) to measure the sensitivity and specificity of the electrochemical reaction test strip 2 (Xin C1f1C1ty). In addition, since the conductive electrode 22 of the connection region 22c needs to be transferred to the electronic or electric material, the conductor has a good conductive gold, which also contributes to the electrochemical reaction test. The sensitivity and specificity of slice 2 in measurement. Therefore, the conductive paste layer 22 can be printed on the substrate 21 at the time of fabrication. After the cleaning and pickling process, the conductive paste layer 220 at the conductive region 22b is sprayed or coated with an insulating varnish 22〇1. Thereby, the conductive colloid layer 220 at the conductive region 22b is not in contact with the reaction solution used for electroless plating in the subsequent electroless plating process, so that the conductive colloid layer 220 at the conductive region 22b is no longer plated with metal. The layer can effectively save the amount of inert metal (such as gold, platinum, palladium) required in the first metal layer 221, the first metal layer 222 and the third metal layer 223 to reduce the manufacturing cost. In addition, the substrate 21, the conductive rubber layer 220, the first metal layer 221, and the second metal layer 222 in the second preferred embodiment are made of a third metal layer 223' and a relatively thin configuration. The descriptions in the first preferred embodiment are substantially the same, and the detailed description thereof will not be repeated here. The present invention further provides a third preferred embodiment, the structure of which is the same as that of the first preferred embodiment, so reference is made to Figs. 1A and 1B. The electrochemical test strip 11 provided in the third preferred embodiment of the present invention comprises a substrate 11 and a conductive electrode 12 laid on the substrate 11, and an insulating layer 13 is provided on the conductive electrode 12. On the other hand, in the configuration of the conductive electrode 12, the conductive colloid layer 120, the first metal layer 12i, the second metal layer 122, and the third metal layer 123 are sequentially stacked from the substrate η. 099135366 Form No. A0101 Page 9 / Total 16 Page 0992061785-0 201217775 [0023] The conductive colloid layer 120 can be composed of copper glue, nickel glue, silver glue, silver carbon glue or carbon glue and the conductive colloid layer 12Q is The brush flow is printed on the substrate 11. The first metal layer 121 is composed of a Group VIII metal, and after the substrate 11 printed with the conductive colloid layer 120 is subjected to a plasma cleaning and pickling process, and then electrolessly deposited on the conductive colloid layer 12 . The first metal layer 122 is composed of nickel. The third metal layer 123 is composed of palladium 'gold or a metal. The second metal layer m and the third metal layer 123 are respectively plated on the first metal layer 12 and the second metal layer 122 by electroless plating. In addition, the first metal layer 121 is a substrate n on which the conductive colloid layer 120 is printed and subjected to plasma cleaning and pickling procedures, and is immersed in an electrolyte containing a Group VIII metal ion to control the temperature, time, and electrolysis of the electrolysis. The first metal layer 121 is plated on the conductive colloid layer 120 in an appropriate range of the ion concentration of the VI quinone metal in the liquid and the pH of the electrolyte. The electroless plating of the second metal layer 122 and the third metal layer 123 is similar to the electroless plating of the first metal layer 121, except when the second metal layer 122 is plated onto the first metal layer 121. The substrate II having the first metal layer 121 on the ore is immersed in the electrolyte of the etched ion, and when the third metal layer 123 is plated on the second metal layer 122, the substrate having the first metal layer 122 is plated. 11 is soaked in an electrolyte containing a pump, gold or a precursor ion and controls the temperature and time of electrolysis and the concentration of metal ions to be plated in the electrolyte and the pH of the electrolyte in an appropriate range. The above description is only the preferred embodiment of the present invention, and is not intended to limit the patent application rights of the present invention. The above description should be understood and implemented by those skilled in the art, and therefore the other is not departing from the present invention. The form bat number A0101 page 10 / total 16 page 0992061785 - [0024] 201217775 The equivalent changes or modifications made under the spirit of the disclosure are to be included in the scope of the patent application. BRIEF DESCRIPTION OF THE DRAWINGS [0025] FIG. 1A is a schematic view of an electrochemical detection test piece according to a first preferred embodiment of the present invention. .
[0026] 第1B圖，為本發明第一較佳實施例電化學檢測試片剖面 示意圖。 [0027] 第2A圖，為本發明第二較佳實施例電化學檢測試片示意 〇 圖。 [0028] 第2B圖，為本發明第二較佳實施例電化學檢測試片刳面 , 示意圖。 【主要元件符號說明】 [0029] 電化學檢測試片1、2 [0030] 基板11、21 [0031] 導電電極12 '22 [0032] 導電膠體層 120 、 220 [0033] 第一金屬層 121 、 221 [0034] 第二金屬層 122 、 222 [0035] 第三金屬層 123 、 223 [0036] 絕緣層13、 23 [0037] 反應材料24 099135366 表單編號A0101 第11頁/共16頁 0992061785-0 201217775 [0038] 反應區 22a [0039] 傳導區 22b [0040] 插入區 22c [0041] 絕緣漆 2201 099135366 表單編號 A0101 第 12 頁/共 16 頁 0992061785-01B is a schematic cross-sectional view showing an electrochemical detection test piece according to a first preferred embodiment of the present invention. 2A is a schematic view of an electrochemical detection test piece according to a second preferred embodiment of the present invention. 2B is a schematic view showing the surface of an electrochemical detection test piece according to a second preferred embodiment of the present invention. [Explanation of main component symbols] [0029] Electrochemical detection test strips 1, 2 [0030] Substrate 11, 21 [0031] Conductive electrode 12 '22 [0032] Conductive colloid layer 120, 220 [0033] First metal layer 121, 221 [0034] Second metal layer 122, 222 [0035] Third metal layer 123, 223 [0036] Insulation layer 13, 23 [0037] Reaction material 24 099135366 Form number A0101 Page 11 / Total 16 page 0992061785-0 201217775 Reaction Zone 22a [0039] Conduction Zone 22b [0040] Insertion Zone 22c [0041] Insulating Varnish 2201 099135366 Form No. A0101 Page 12 of 16 0992061785-0