TW201212828A - Nutritional products including pea protein hydrolysates - Google Patents
Nutritional products including pea protein hydrolysates Download PDFInfo
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- TW201212828A TW201212828A TW100130367A TW100130367A TW201212828A TW 201212828 A TW201212828 A TW 201212828A TW 100130367 A TW100130367 A TW 100130367A TW 100130367 A TW100130367 A TW 100130367A TW 201212828 A TW201212828 A TW 201212828A
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- protein
- nutritional
- weight
- hydrolysate
- product
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- 229940091258 selenium supplement Drugs 0.000 description 1
- 230000035910 sensory benefits Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- XGVXKJKTISMIOW-ZDUSSCGKSA-N simurosertib Chemical compound N1N=CC(C=2SC=3C(=O)NC(=NC=3C=2)[C@H]2N3CCC(CC3)C2)=C1C XGVXKJKTISMIOW-ZDUSSCGKSA-N 0.000 description 1
- 150000003385 sodium Chemical class 0.000 description 1
- COEZWFYORILMOM-UHFFFAOYSA-M sodium 4-[(2,4-dihydroxyphenyl)diazenyl]benzenesulfonate Chemical compound [Na+].OC1=CC(O)=CC=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 COEZWFYORILMOM-UHFFFAOYSA-M 0.000 description 1
- BBMHARZCALWXSL-UHFFFAOYSA-M sodium dihydrogenphosphate monohydrate Chemical compound O.[Na+].OP(O)([O-])=O BBMHARZCALWXSL-UHFFFAOYSA-M 0.000 description 1
- 239000012265 solid product Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 235000003687 soy isoflavones Nutrition 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- MZNYADCLSDCSHV-UHFFFAOYSA-N sulfuric acid;zinc;hydrate Chemical compound O.[Zn].OS(O)(=O)=O MZNYADCLSDCSHV-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 235000020209 toddler milk formula Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 235000012773 waffles Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 235000010930 zeaxanthin Nutrition 0.000 description 1
- 229940043269 zeaxanthin Drugs 0.000 description 1
- 239000001775 zeaxanthin Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229940118149 zinc sulfate monohydrate Drugs 0.000 description 1
- RNZCSKGULNFAMC-UHFFFAOYSA-L zinc;hydrogen sulfate;hydroxide Chemical compound O.[Zn+2].[O-]S([O-])(=O)=O RNZCSKGULNFAMC-UHFFFAOYSA-L 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/40—Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
Description
201212828 六、發明說明: 【發明所屬之技術領域】 本發明係關於包含碗豆蛋白水解物之營養產品。更特定 吕之,本發明係關於包含婉豆蛋白水解物之嬰兒、兒童及 成人營養產品,其具有降低之免疫反應性及改良之風味及 感官性質,包括乳液穩定性。 【先前技術】 為成人、兒童及嬰兒製造之包含營養成分之目標選擇的 營養液及營養粉為吾人所熟知且廣泛可用,其_一些可提 供唯一營養來源,而其他可提供補充來源。此等營養品包 括可用水或其他水性液體復原之粉末,以及濃縮及即飲營 養液,諸如基於乳或蛋白質之乳液或非乳化或實質上澄清 液體。當調配成含有所選營養成分時,此等營養液尤其適 用。 許多個體、尤其嬰兒(包括早產兒)經歷對液體及粉末中 〇 之特定營養成分不耐(配方不耐)。術語「不耐」與「配方 不耐」在本文中可互換使用。不耐為非免疫系統相關反應 且可由行為或排便或進食模式變化來證實,諸如與對營養 - 《分耐受良好之個體相比"區吐增加、排便次數增加或水 . _便。不耐最通常由胃腸症狀(例如β區吐、排便模式及 氣體)以及行為特性(例如配方接受度、焦燥及味哭)指示。 不耐可與一些個體、尤其嬰兒對特定營養組合物所展現 :過敏型反應形成對照。此等過敏型反應與免疫系統相 關,且可由個體對營養品中存在之蛋白質(一般為牛乳蛋 158197.doc 201212828 白)的敏感引起。此等免疫系統相關之過敏或敏感通常導 致皮膚、呼吸道或胃腸症狀,諸如》區吐及腹填。 許多展現對牛乳蛋白過敏或敏感之個體能夠利用基於大 豆蛋白、尤其大豆蛋白分離物之營養產品。 基於水解物之配方(其中一些可為低過敏性)含有已水解 或分解為短肽片段及胺基酸且因此實質上免疫失活之蛋白 質。展現對牛乳及大豆蛋白之反應的個體通常不會對水解 物配方作出反應,因為其免疫系統不會將水解蛋白質識別 為引起其症狀之完整蛋白質。如先前所述,展現對包括牛 乳及/或大豆蛋白之營養組合物之免疫系統相關反應的個 體亦可展現非免疫系統相關反應(配方不对)。 儘管基於經廣泛水解之蛋白質或胺基酸混合物之產品一 般可為對牛乳及大豆蛋白過敏之個體所接受,但其具有相 當大的味道及感官問題。雖然已多次試圖藉由使用水解蛋 白質、尤其水解大丑蛋白製備營養組合物來避免配方過敏 及不耐,但先前尚不清楚如何使用水解蛋白質調配營養產 品而不會顯著不利地影響產品之感官性質,尤其是味道。 舉例而言’使用水解蛋白質通常產生容易自溶液分離及/ 或沈澱出且此外具有苦味、喊味、酸味及/或豆味之營養 因此,本發明係關於包含碗 主要或唯一來源且呈營養液、 產品及其他食品。該豌豆蛋白 品在存放期内為物理上穩定的 豆蛋白水解物作為蛋白質之 營養粉、營養棒形式之營養 水解物具有使得所得營養產 且提供有利感官性質、同時 158197.doc 201212828 保持實質上免疫失活之水解度。營養產品亦可用作對除婉 蛋白外之蛋白質不耐及/或過敏及/或具有吸收障礙、消 化不良或其他胃腸病狀之個體的營養來源。 【發明内容】 =實施例為一種營養產品,其包括豌豆蛋白水解物,該 ' 豌五蛋白水解物之經調整水解度為約7.0。/。至小於19.0%, 匕括介於約8.0%與約16〇%之間,包括介於約8%與約 0 13.5/❶之間,且亦包括介於約12.0%與16.0%之間。另外, 在實施例中,豌豆蛋白水解物中小於5%之蛋白質的分 子篁大於3 kDa。產品呈選自由以下組成之群的形式:液 體飲料(例如營養乳液、實質上澄清營養液及凝膠)、粉 末食°0、營養棒及營養補充劑。希望產品為嬰兒配方。 另實施例為一種營養產品’其包括具有以每1公克蛋 白質计小於15,〇〇〇叫免疫活性豌豆抗原之豌豆蛋白水解 物。 〇 另一實施例為一種製造營養產品之方法,該方法包含組 合蛋白質、脂肪及碳水化合物。該營養產品包括經調整水 解度介於約7.0%與小於19 〇%之間的豌豆蛋白水解物作為 其蛋白質之主要或唯一來源。在一實施例中,豌豆蛋白水 -解物中小於5%之蛋白質的分子量大於3 kDa。在另—實施 例中,豌豆蛋白水解物具有以每丨公克蛋白質計小於 15,000 pg免疫活性豌豆抗原。 如本文所述之營養產品包含豌豆蛋白水解物作為蛋白質 之唯一或主要來源,其不僅向對牛乳蛋白及/或大豆蛋白 158197.doc 201212828 不耐及/或過敏之個體提供營養來源,而且為物理上穩定 的及/或在每一所選產品形式下均具有感官益處 所述之許多實施例中,碗豆蛋白水解物為蛋白質之唯 源以使得營養產品為低過敏性且適合用於食物過敏患者。 現已發現,此等不耐及/或過敏問題可藉由調配:有瘦 調整水解度介於約7.0%與小於19.0%之間的碗豆蛋白水解 物之營養產品來減少或消除,該水解度產生特定分子量概 況及抗原含量。特定言之,已發現使用此等碗豆蛋白水解 物可降低免疫活性。此外,與包括其他水解植物蛋白質來 源之營養產品b匕’經調整水解度介於約7 〇%與小於 19.0%之間的豌豆蛋白水解物提供改良風味及其他改良感 官性質。因此,現在有可能利用本發明之水解豌豆蛋2製 造基於味道可口、低過敏性、耐受水解物之營養品。 【實施方式】 本文所述之營養產品包含豌豆蛋白水解物作為蛋白質來 源。在許多實施例中,營養產品包含豌豆蛋白水解物作為 蛋白質之唯一來源以使得營養產品為低過敏性且適合用於 食物過敏患者。下文詳細描述營養產品之此等及其他美本 特徵,以及一些許多視情況存在之變化及添加。 除非另外規定’否則如本文中所使用之術語「低過敏 性」係指營養產品在小於1 0%之食物過敏個體中引起對食 物之過敏反應症狀。 除非另外規定,否則如本文中所使用之術語「實質上澄 清營養液」係指具有明顯澄清或半透明外觀之非乳化或類 158197.doc 201212828 似其他液體,該液體可具有且通常將具有稀的或水樣紋 理,其稠度類似於澄清果汁,且如由布絡克菲爾德黏度計 (Br〇〇kfield viscometer^22t下使用丨號軸在6〇『㈣下所 測定,最通常黏度小於約25厘泊。201212828 VI. Description of the Invention: TECHNICAL FIELD OF THE INVENTION The present invention relates to a nutritional product comprising a tofu protein hydrolysate. More specifically, the present invention relates to infant, child and adult nutritional products comprising cowpea protein hydrolysate having reduced immunoreactivity and improved flavor and organoleptic properties, including emulsion stability. [Prior Art] Nutrients and nutritional powders selected for the purpose of nutrition, which are made for adults, children and infants, are well known and widely available, some of which provide a single source of nutrition, while others provide supplemental sources. Such nutraceuticals include powders that can be reconstituted with water or other aqueous liquids, as well as concentrated and ready-to-drink nutrients such as milk or protein based emulsions or non-emulsified or substantially clear liquids. These nutrient solutions are especially useful when formulated to contain selected nutrients. Many individuals, especially babies (including premature babies), experience intolerance to specific nutrients in liquids and powders (formulation intolerance). The terms "intolerance" and "formulation intolerance" are used interchangeably herein. Impatient is a non-immune system-related response and can be confirmed by behavioral or changes in bowel movements or eating patterns, such as an increase in the amount of vomiting, an increase in the number of bowel movements, or water. Impatientness is most often indicated by gastrointestinal symptoms (such as beta vomiting, defecation pattern, and gas) and behavioral characteristics (such as formula acceptance, astringency, and astringent crying). Intolerance can be demonstrated with certain individuals, especially infants, for specific nutritional compositions: allergic reactions form a control. These allergic reactions are associated with the immune system and can be caused by the individual's sensitivity to the protein present in the nutrient (generally the milk egg 158197.doc 201212828 white). Allergies or sensitivities associated with such immune systems often result in skin, respiratory or gastrointestinal symptoms such as vomiting and abdominal filling. Many individuals exhibiting allergy or sensitivity to cow's milk protein are able to utilize nutritional products based on soy protein, especially soy protein isolates. Formulations based on hydrolysates (some of which may be hypoallergenic) contain proteins which have been hydrolyzed or decomposed into short peptide fragments and amino acids and are therefore substantially immunologically inactivated. Individuals exhibiting a response to milk and soy protein typically do not respond to the hydrolyzate formulation because their immune system does not recognize hydrolyzed proteins as intact proteins that cause their symptoms. As previously described, individuals exhibiting an immune system-related response to a nutritional composition comprising cow's milk and/or soy protein may also exhibit a non-immune system related response (incorrect formula). While products based on widely hydrolyzed proteins or mixtures of amino acids are generally acceptable to individuals who are allergic to cow's milk and soy protein, they have considerable taste and sensory problems. Although many attempts have been made to avoid formulation allergies and intolerance by using hydrolyzed proteins, especially hydrolyzed ugly proteins, to prepare nutritional compositions, it is not clear how to use hydrolyzed proteins to formulate nutritional products without significantly adversely affecting the sensory of the product. Nature, especially the taste. For example, the use of hydrolyzed proteins generally produces a nutrient that is easily separated and/or precipitated from the solution and which additionally has a bitter, shoddy, sour and/or beany taste. Accordingly, the present invention relates to a nutrient solution comprising a bowl of primary or sole source , products and other foods. The pea protein product is a physically stable soybean protein hydrolysate during the storage period as a nutrient powder of protein, and a nutrient hydrolysate in the form of a nutrient bar, which has the resulting nutrient production and provides favorable organoleptic properties, while maintaining substantial immunity in 158197.doc 201212828 Inactivation of hydrolysis. The nutritional product can also be used as a source of nutrition for individuals who are intolerant and/or allergic to proteins other than prion protein and/or have malabsorption, digestive or other gastrointestinal conditions. SUMMARY OF THE INVENTION = The example is a nutritional product comprising a pea protein hydrolysate having an adjusted degree of hydrolysis of about 7.0. /. To less than 19.0%, including between about 8.0% and about 16%, including between about 8% and about 0.35/❶, and also between about 12.0% and 16.0%. Additionally, in the examples, less than 5% of the protein in the pea protein hydrolysate has a molecular weight greater than 3 kDa. The product is in the form of a group selected from the group consisting of liquid beverages (e.g., nutritional emulsions, substantially clarified nutrient solutions and gels), powdered foods, nutritional bars, and nutritional supplements. I hope the product is an infant formula. Another embodiment is a nutritional product' which comprises a pea protein hydrolysate having less than 15 grams per gram of protein, nicknamed immunologically active pea antigen.另一 Another embodiment is a method of making a nutritional product comprising combining protein, fat, and carbohydrates. The nutritional product comprises a pea protein hydrolysate having an adjusted degree of hydrolysis between about 7.0% and less than 19% as the primary or sole source of protein. In one embodiment, less than 5% of the protein in the pea protein water-solution has a molecular weight greater than 3 kDa. In another embodiment, the pea protein hydrolysate has less than 15,000 pg of immunologically active pea antigen per gram of protein. The nutritional product as described herein comprises pea protein hydrolysate as the sole or primary source of protein, which not only provides a source of nutrition to individuals who are intolerant and/or allergic to cow's milk protein and/or soy protein 158197.doc 201212828, but also physics In many embodiments that are stable and/or have sensory benefits in each of the selected product forms, the soy protein hydrolysate is the only source of protein to make the nutritional product hypoallergenic and suitable for food allergy. patient. It has been found that such intolerance and/or allergy problems can be reduced or eliminated by formulating a nutrient product having a lean adjusted degree of hydrolysis between about 7.0% and less than 19.0%. Degree produces a specific molecular weight profile and antigen content. In particular, it has been found that the use of such bean protein hydrolysates reduces immunological activity. In addition, the pea protein hydrolysate having an adjusted degree of hydrolysis of between about 7% and less than 19.0% of the nutritional product, including other hydrolyzed vegetable protein sources, provides improved flavor and other improved sensory properties. Therefore, it is now possible to produce a nutrient based on taste, hypoallergenic, and hydrolyzed tolerant using the hydrolyzed pea egg 2 of the present invention. [Embodiment] The nutritional product described herein comprises a pea protein hydrolysate as a protein source. In many embodiments, the nutritional product comprises pea protein hydrolysate as the sole source of protein to render the nutritional product hypoallergenic and suitable for use in food allergy sufferers. These and other aesthetic features of the nutritional product are described in detail below, as well as a number of variations and additions as appropriate. The term "hypoallergenic" as used herein, unless otherwise specified, means that the nutritional product causes symptoms of an allergic reaction to food in less than 10% of food allergic individuals. The term "substantially clear nutrient solution" as used herein, unless otherwise specified, refers to a non-emulsifying or 158197.doc 201212828-like other liquid having a distinctly clear or translucent appearance, which liquid may have and will typically have a Or watery texture, the consistency is similar to that of clarified juice, and as measured by a Brookfield viscometer (Br〇〇kfield viscometer^22t using a 丨 axis at 6〇“(4), the most common viscosity is less than about 25 PCT. Parking.
除非另外規^ ’否則如本文中所使用之術語「嘴霧乾燥 營養粉」係指營養粉中之大多數組分(包括碗豆蛋白水解 物)已在製造期間經均質化且隨後進行嘴霧乾燥製程。其 他成分可經由乾式摻合添加至噴霧乾燥粉末中,只要至少 豌豆蛋白水解物已預先均質化及喷霧乾燥即可。 ^ 除非另外規定’否則如本文中所使用之術語「經調整水 解度」係'才旨已經調整或校正以說明完整蛋白質物質之可量 測胺基氮含量的指;t蛋白質物質卜般為婉豆蛋白物質)之 水解度。舉例而言’若完整的未經修飾之蛋白質物質的胺 基氮含量為8.68且已進行水解程序之蛋白質的水解度為 16.68,則水解蛋白質之經調整水解度為8 〇〇。 除非另外規定,否則如本文中所使用之術語「脂肪」及 「油」可互換用於指來源於植物或動物或自植物或動物加 工之脂質物質。此等術語亦包括合成脂質物質,只要該等 合成物質適合於經口投與至人類即可。 術語「人乳寡畴」或「HM0」—般係指存在於人乳中之 多種複合碳水化合物。存在於乳募醣中之單體通常包括& 葡萄糖(Glc)、D-半乳糖(Gal)、N-乙醯葡糖胺(GicNAC)、 L-岩藻糖(Fuc)及唾液酸[N_乙醯神經胺酸(NeuAc)]。此等 單體之延長可藉由使以01_3或01_4鍵連接之GlcNAc殘基連 158197.doc 201212828 接於Gal殘基,隨後進一步以卜丨^或^丨一鍵添加Gal來達 成。大部分HMO在其還原端帶有乳糖。由此等單體可形成 許多核心結構。由於連接乳糖胺、卩此及/或NeuAc可產生 其他變化形式。 除非另外規定,否則如本文中所使用之術語「存放穩 定」係指營養產品在18-24°C下封裝及隨後儲存至少3個月 後(包括約6個月至約24個月,且亦包括約12個月至約18個 月)保持商品穩定。 如本文中所使用,術語「營養組合物」或「營養產品」 可互換使用且除非另外規定,否則係指營養液、營養粉、 營養棒、營養補充劑及如此項技術中已知之任何其他營養 食品。營養粉可復原形成營養液,其均包含脂肪、蛋白質 及碳水化合物中之一或多者且適合於人類口服食用。 「營養液」 及藉由在使 除非另外規定’否則如本文中所使用之術語 係指呈即飲液體形式、濃縮形式之營養產品, 用之前復原本文所述之營養粉所製得的營養液 除非另外規定,否則如本文中所使 σ JXj j 指12個月或12個月以下之旮土 ,, 乃Λ卜之兒里。如本文中所使用之術言 幼兒⑽叫」係指6個月至3歲之兒童。如本文 用之術語「兒童」制自出生至13歲之使用者。如本 所使用之術語「早產兒」料在絲㈣以 除非另外規定,否刖1 士 ^ 女兄0 否則如本文中所使用之所有百分比、伤 數及比率均以總叙合物之舌θ >丄 有該等重量…: 十。除非另外規定’否則所 在”關於所列成分時係基於有效含量且因此不 158197.doc 201212828 匕括可能包括於市售物質中之溶劑或副產物。 除非另外規定或所提及之上下文相反地明確暗示,否則 所有對本發明之單數個特性或限制的提及均應包括相應的 複數個特性或限制,反之亦然。 〜 除非另外規定或提及組合之上下文相反地明確暗示,否 則如本文中所使用之方法或製程步驟之所有組合均可以任 何順序進行。The term "mouth-dried dry nutrient powder" as used herein, unless otherwise specified, means that most of the components of the nutritional powder, including the bean protein hydrolysate, have been homogenized during manufacture and subsequently dried by mouth. Process. Other ingredients may be added to the spray dried powder via dry blending as long as at least the pea protein hydrolysate has been previously homogenized and spray dried. ^ Unless otherwise specified, 'the term "adjusted degree of hydrolysis" as used herein is a measure that has been adjusted or corrected to account for the measurable amine-based nitrogen content of intact proteinaceous matter; The degree of hydrolysis of the soy protein material). For example, if the intact unmodified protein material has an amine nitrogen content of 8.68 and the degree of hydrolysis of the protein subjected to the hydrolysis procedure is 16.68, the hydrolyzed protein has an adjusted degree of hydrolysis of 8 Å. The terms "fat" and "oil" as used herein are used interchangeably to refer to a lipid material derived from a plant or animal or from a plant or animal, unless otherwise specified. These terms also include synthetic lipid materials as long as the synthetic materials are suitable for oral administration to humans. The term "human milk oligodomain" or "HM0" generally refers to a plurality of complex carbohydrates present in human milk. The monomers present in the lactose usually include & glucose (Glc), D-galactose (Gal), N-acetylglucosamine (GicNAC), L-fucose (Fuc), and sialic acid [N _ acetaminogen (NeuAc)]. The extension of these monomers can be achieved by attaching a GlcNAc residue linked by a 01_3 or 01_4 bond to 158197.doc 201212828 to a Gal residue, and then further adding Gal by a bond or a key. Most HMOs carry lactose at their reducing end. Thus, the monomer can form a plurality of core structures. Other variations can be made by the attachment of lactosamine, hydrazine and/or NeuAc. The term "storage stable" as used herein, unless otherwise specified, means that the nutritional product is packaged at 18-24 ° C and subsequently stored for at least 3 months (including from about 6 months to about 24 months, and also Includes approximately 12 months to approximately 18 months) to maintain commodity stability. As used herein, the terms "nutritional composition" or "nutraceutical product" are used interchangeably and, unless otherwise specified, refer to nutrient solutions, nutritional powders, nutritional bars, nutritional supplements, and any other nutrients known in the art. food. The nutritional powder can be reconstituted to form a nutrient solution, each of which contains one or more of fat, protein and carbohydrates and is suitable for human oral consumption. "Nutrition solution" and nutrient solution prepared by restoring the nutrient powder described herein by using the terminology in a ready-to-drink liquid form, a concentrated form, unless otherwise specified as otherwise. Unless otherwise specified, σ JXj j as used herein refers to bauxite of 12 months or less, which is included in the children. As used herein, a child (10) is a child between 6 months and 3 years of age. As used herein, the term "child" is used by users from birth to age 13. The term "premature babies" as used herein is intended to be in the silk (4) unless otherwise specified, otherwise 刖1 士^女兄0 otherwise all percentages, injuries and ratios used herein are in the total syllable tongue θ >丄There are these weights...: Ten. Unless otherwise specified, 'otherwise' is based on the effective amount and therefore does not include 158197.doc 201212828, including solvents or by-products that may be included in commercially available materials. Unless otherwise stated or the context It is suggested that all references to the singular number of features or limitations of the invention are intended to include a plurality of features or limitations, and vice versa. </ RTI> Unless otherwise specified or referred to in the context of the combination, All combinations of methods or process steps used can be performed in any order.
本發明之營養產品的各種實施例亦可實質上不含本文所 述之任何視情況選用或所選的基本成分或特徵,其限制條 件為剩餘營養產品仍含有如本文所述之所有所需成分或特 徵。在本文中且除非另外規定,否則術語「實質上不含 明所選營養產品含有小於功能量之視情況選用的成分, 通常小於約1重量% ’包括小於約〇.5重量%,包括小於約 0.1重量%且亦包括0重量%的該視情況選用或所選的美 成分。 土 〇 營養產品可包含如本文所述之產品的基本要素以及本文 所述或另外適用於營養產品應用u壬何其他或視情況選用 之要素,由其組成,或基本上由其組成。 產品形式 包括豌丑蛋白水解物之本發明的營養組合物可以任何已 知或另外適合之口服產品形式調配及投與。任何固體、液 體或粉末形式(包括其組合或變化形式)適用於本文,其限 制條件為所述形式允許安全且有效地將如本文亦定義之基 本成分經口傳遞至個體。 158197.doc 201212828 本發明之營養組合物包括如本文所述之豌豆蛋白水解 物。組合物可包括婉豆蛋白水解物作為蛋白質之唯一來源 (即碗豆蛋白水解物為組合物之1〇〇%蛋白質組分)或可包括 婉豆蛋白水解物與如本文所述之其他水解、部分水解或未 水解(完整)蛋白質組合。組合物包括任何產品形式,其包 含本文所述之基本成分,且對於經口投藥安全且有效。營 養組合物可魏成僅包括本文料之基本成分,或可用: 情況之成分修飾以形成多種不同產品形式。本發明之營養 組合㈣佳調配賴食產品形式,其在本文中定義為:如 下產品形式包含本發明之基本成分的實施例:其含有脂 肪、蛋白質及碳水化合物中之至少一者,且較佳亦含有= 生素、碌物質或其組合。在許多實施例中,組合物將包含 作為蛋白質之唯一來源的豌豆蛋白水解物與脂肪、碳水化 口物、准生素及礦物質組合以產生低過敏性組合物。一個 特定實例為低過敏性嬰兒配方,其包括1〇〇%豌豆蛋白水 解物作為蛋白質來源、脂肪、碳水化合物、維生素及礦物 質。 因此’本發明之營養組合物可包括多種不同產品形式, 包括大部分任何習知或另外已知的食品形式,其中—此非 限制性實例包括糖果產品、穀類、食物調味料(例如塗抹 醬(spreads)、粉末、醬料、果醬、果凍、咖啡奶精或甜味 劑)、麵食(pasta)、烘焙或烹調物質(例如麵粉、脂肪咬 ’由、奶油或人造奶油 '裹粉(breading)或烘培混料)、越、責 或調味點心(seasoned snacks)(例如擠製、烘培、油炸)、飲 158I97.doc -10- 201212828 料(例如咖啡、果汁、碳酸飲料、非碳酸飲料、茶)、點心 或代餐棒(例如 SlimfastTM棒、Ensure™棒、Zone perfect™ 棒、)、思慕昔(srn〇〇thies)、早餐縠類、乳 酷·、果汁軟糖產品(gummie products)、加鹽或未加鹽鬆脆 點心(例如薄片、脆餅、普澤餅(pretzels))、調味汁(dips)、 ^ 烘焙食品(例如餅乾、蛋糕、餡餅、糕餅、麵包、貝果 (bagels)、脆麵包丁(croutons)、調味醬、乾混料(例如用於 ◎ 馬芬(muffins)、餅乾、鬆餅(waffles)、薄煎餅、飲料之混 料)、冷凍甜點(例如冰淇淋、冰棒、富奇軟糖棒(fudge bars)、碎冰、冷凍優格)' 麵食、加工肉食(例如玉米熱 狗、漢堡、熱狗、香腸、紅椒香腸)、比薩、布丁、經調 味或未經調味之明膠、冷藏麵團(例如餅乾、麵包、堅果 巧克力糕(brownies))、優格或基於優格之飲料、冷凍優 格、湯、基於蔬菜之漢堡及基於爆米花之點心。 本發明之營養組合物亦可以諸如以下之產品形式調配: 〇 膠囊、錠劑、丸劑、囊片、凝膠、液體(例如懸浮液、溶 液、乳液、澄清溶液)、粉末或其他微粒等。此等產品形 式一般僅含有如本文所述之基本成分,視情況與其他活性 劑、加工助劑或其他劑型賦形劑組合。 營養產品包括液體及喷霧乾燥粉末形式,其包括即食乳 化液體及在使狀前可用水或其他水性液體稀釋形成營養 液之喷霧乾燥可復原粉末。 特定言之,營養組合物可為用於對基於牛乳蛋白或大豆 蛋白之配方不耐之早產兒的人乳強化劑,用於對牛乳蛋白 158197.doc •11· 201212828 或大豆蛋白過敏之嬰兒的人乳強化劑,用於對基於牛乳蛋 白或大豆蛋白之配方不耐之早產兒的早產兒配方,或用於 對牛乳蛋白或大豆蛋白過敏之早產兒的早產兒配方,用於 對基於牛乳蛋白或大豆蛋白之配方不耐之嬰兒的嬰兒配 方’用於對牛乳蛋白或大豆蛋白過敏之嬰兒的嬰兒配方, 用於具有一般食物過敏之嬰兒的低過敏性配方,用於對基 於牛乳蛋白或大豆蛋白之配方不耐之嬰兒的較大嬰兒配方 (f〇U〇W_on infant formulas),用於對基於牛乳蛋白或大豆 蛋白之配方過敏之嬰兒的較大嬰兒配方,用於對基於牛乳 蛋白或大豆蛋白之配方不耐之6個月以上嬰兒的幼兒配 方,用於對基於牛乳蛋白或大豆蛋白之配方過敏之6個月 以上嬰兒的幼兒配方,用於對基於牛乳蛋白或大豆蛋白之 呂養°°不耐之兒童及成人的營養飲料,用於對基於牛乳蛋 白或大豆蛋白之營養品過敏之兒童及成人的營養飲料,用 於對基於牛乳蛋白或大豆蛋白之營養品不耐之老年患者的 營養飲料,用於對基於牛乳蛋白或大豆蛋白之營養品過敏 之^年患者的營養飲料,用於對基於牛乳蛋白或大豆蛋白 之營養品不耐之人群的運動營養飲料,用於對基於牛乳蛋 白或大丑蛋白之營養品過敏之人群的運動營養飲料使用 具有改良感官性質之水解蛋白質的運動營養飲料,使用呈 有改良感官性質之水解蛋白質的營養棒,用於具有吸收障 礙4化不良及其他胃腸病狀之患者之營養需求的半要素 -方及配方、棒狀物、液體或粉末狀蛋白補充劑 需要水解蛋白質來管理盆膦舍串 ,、膳&之患者所没計的基於水解蛋 158197.doc -12- 201212828 白質之其他食物形式。 當本發明之營養組合物調配成膳食產品形式時,其可能 向個體提供唯一營養來源或補充營養來源^在本文中,唯 一營養來源為可每天投與一次或多次以可能向個體提供每 天或預期投藥期間的所有或實質上所有其脂肪、蛋白質、 - 碳水化合物、礦物質及維生素需求。補充營養來源在本文 中定義為不向個體提供潛在唯一營養來源的膳食來源。 液體飲料 Ο 祕 液體飲料包括濃縮及即食液體。適用於本文之液體產品 形式的非限制性實例包括液體嬰兒配方(包括即食調配物 與濃縮調配物)、液體幼兒配方(包括即食調配物與濃縮調 配物)、液體人乳強化劑(包括即食調配物與濃縮調配物)、 點心及代餐產品、熱飲或冷飲、碳酸或非碳酸飲料、果汁 或其他酸化飲料、混合飲料(shake)、咖啡、茶、腸道餵養 組合物等。此等液體組合物最通常調配成懸浮液或乳液, 〇 但亦可以任何其他適合之形式調配,諸如澄清液體溶液、 液體凝膠等。 本發明之營養液飲料可用作用於對基於牛乳蛋白及/或 2且蛋白之營養品不耐或過敏之早產兒、嬰兒、幼兒、兒 里及成人(包括老年患者)的營養飲料。液體飲料亦可用作 用於具有吸收障礙、消化不良及其他胃腸病狀之患者之營 養需求的半要素配方。 A.營養乳液 營養乳液包含碗豆蛋白水解物作為蛋白質之主要或唯- 158197.doc -13- 201212828 來源且為進-步包含脂肪及碳水化合物之水性乳液。此等 乳液在約rc至約2rc下為可流動或可飲用液體且通常呈 水包油、油包水或複合水性乳液形式,但該等乳液最通常 呈具有連續水相及不連續油相之水包油乳液形式。 營養乳液可為且通常為存玫穩定的。以營養乳液之重量 計,營養乳液通常含有至多約95重量%之水,包括約5〇% 至約95%’亦包括約60%至約9〇%,且亦包括約7〇%至約 8 5 %之水。 營養乳液可調配成含有足夠種類及量之營養素以提供唯 一、主要或補充營養來源,或提供用於罹患特定疾病或病 狀之個體的專用營養乳液。舉例而言’可生產用作用於對 基於牛乳蛋白及/或大豆蛋白之營養品不耐或過敏之使用 者的嬰兒、較大嬰兒及幼兒配方、成人配方及其類似物之 營養乳液。在另一實施例中,乳液可用作用於具有一般食 物過敏之個體的低過敏性營養乳液。因此,此等營養乳液 可具有多種產品密度,但最通常密度大於約丨〇3 g/ml,包 括大於約1.04 g/ml,包括大於約Low g/ml,包括約丨〇6 ^ g/ml至約i.12 g/ml ’且亦包括約i 〇85 g/mi至約i 1〇 g/ml ° 營養乳液可具有適合最終使用者之營養需求的熱量密 度’但在大多數情況下’乳液包含約1〇〇 kcal/24〇 mi至約 500 kcal/240 ml ’ 包括約 150 kcal/240 ml至約 350 kcal/240 m卜且亦包括約200 kcal/240 ml至約320 kcal/240 m卜 在一替代實施例中’營養乳液為低熱量營養乳液且熱量 158197.doc •14· 201212828 密度為約 6.5 kcal/fl oz(52.8 kcal/24〇 ml)。在另一實施例 中,營養乳液之熱量密度為約kcal/fl 〇z(57 6 keal/24() m1)。在一實施例中,營養乳液之熱量密度為約77 kcal/fl 〇ζ(62·4 kcal/240 ml)。 • 營養乳液之PH值可在約3.5至約8之範圍内,但最宜在約 - 4.5至約7.5之範圍内,包括約5.5至約7.3,包括約62至約 7.2。 0 儘官營養乳液之份量大小(serving size)可視許多變數而 變化,但典型份量大小在约1〇〇 ml至約3〇〇 W之範圍内, 包括約150 ml至約250 ml,且包括約19〇如至約24〇 mi。 在一實施例中,營養乳液可用作人乳強化劑。在該等實 施例中,典型單個份量大小包括約5.〇 ml。 B.實質上澄清液體飲料 本發明之實質上澄清營養液為至少包含蛋白質及如下文 所論述之碳水化合物的稀液體,該等蛋白質之至少一部分 〇 為婉豆蛋白水解物。實質上澄清營養液實質上不含脂肪; 亦即,除了原料固有之㈣或以低濃度添加以幫助製造液 體之脂肪,液體不添加脂肪。在本文中,術語「不含脂 肪」意謂液體通常含有以營養液之重量計小於1()%、更通 常小於0.5%且更通常小於〇.1%、包括〇%的脂肪。此等實 質上澄清營養液在約至約25<t下為可流動或可飲用液 體。 貫質上澄清營養液可為且通常為存放穩定的。以實質上 澄清營養液之重量計,營養液通常含有至多約95重量%之 158I97.doc -15- 201212828 水,包括約50%至約95%,亦包括約60%至約90%,且亦包 括約70%至約85%之水。 實質上澄清營養液可調配成含有足夠種類及量之營養素 以提供補充營養來源,或提供用於罹患特定疾病或病狀之 個體的專用營養液。舉例而言,在一實施例中,實質上澄 清營養液為用於對基於牛乳蛋白及/或大豆蛋白之營養品 不耐或過敏之使用者的運動飲料。在另一實施例中,實質 上澄清營養液為包括具有改良感官性質之水解蛋白質的運 動飲料。因此,此等實質上澄清營養液可具有多種產品密 度’但最通常密度大於約1.040 g/ml,包括1.06 g/ml至1.12 g/ml,且亦包括約 1.085 g/ml至約 1 1〇 g/ml。 實質上澄清營養液可具有適合最終使用者之營養需求的 熱里禮度’但在大多數情況下’液體包含約9〇 kcal/240 ml 至約 500 kcal/240 ml,包括約 150 kcal/240 ml 至約 350 kcal/240 ml’ 包括約 i8〇 kcal/240 ml 至約 350 kcal/240 ml 且亦包括約250 kcal/240 ml至約320 kcal/240 ml。在其他 實施例中’實質上澄清營養液包含約9〇 kcal/48〇 ml至約 500 kcal/480 m卜包括約 150 kcal/480 ml至約 350 kcal/480 m卜且亦包括約 25〇 kcai/48〇 ml至約 32〇 kcal/48〇 。 貫質上澄清營養液之pH值在約2.8至約4.6之範圍内,包 括約2.9至約4.2,且亦包括約3·1至約3.9。 儘官實質上澄清營養液之份量大小可視許多變數而變 化,但典型份量大小在約1 〇〇 ml至約591 ml之範圍内,包 括約150 mi至約25〇 m卜且包括約19〇爪丨至約24〇爪卜實 158197,doc -16· 201212828 質上澄清營養液之一些特定份量大小包括240 ml(8」盘 司)、296 ml(10盘司)及 480 ml(16盎司)。 營養粉 營養粉包含婉豆蛋白水解物作為蛋白質之主要或唯一來 源且通常呈可流動或實質上可流動微粒組合物或至少微粒 組合物形式。尤其適合之產品形式包括喷霧乾燥、聚結或 乾式摻合粉末組合物。該等組合物可容易用匙或類似其他 0 裝置舀取及量測,其中組合物可容易由預期使用者用適合 之水性液體(通常為水)復原,形成供立即口服或腸道使用 之營養調配物。在本文中,「立即」使用一般意謂在約48 小時内’最通常在約24小時内,較佳在復原後立刻。 營養粉可調配成含有足夠種類及量之營養素以提供唯 一、主要或補充營養來源,或提供用於罹患特定疾病或病 狀之個體的專用營養粉。在一特定實施例中,營養粉可調 配用於對基於牛乳蛋白及/或大豆蛋白之營養品不耐或過 ◎ 敏之個體。在另一實施例中,乳液可用作用於具有一般食 物過敏之個體的低過敏性營養乳液。 營養粉可在使用之前用水復原至適合最終使用者之營養 需求的熱量密度,但在大多數情況下’粉末用水復原形成 包含約 100 kcal/240 ml 至約 500 kcal/240 ml、包括約 150 kcal/240 ml至約 350 kcal/240 ml且亦包括約 200 kcal/240 ml至約320 kcal/240 ml之組合物。 在一替代實施例中,營養粉可用水復原形成熱量密度為 約52.8 kcal/240 ml之低熱量組合物。在另一實施例中,營 158197.doc -17- 201212828 養粉之熱量密度為約57.6 kcal/240 m卜在—實施例中,營 養粉之熱量密度為約62.4 kcal/240 m卜 儘=復原營養液之份量大小可視許多變數而變化,但典 型份量大小在約1〇〇 ml至約3〇〇 ml之範圍内包括約WO 〇11至約25〇1111’包括約19()1111至約24()1111。 在一特^實施财,復原營養液可用作人乳強化劑。在 該等實施例中,典型單個份量大小在每約⑽Μ至約3〇〇 ml水中包括約0.9公克粉末。 其他固趙營養差品 一或者,營養產品可呈固體營養棒及食品形式。舉例而 言,營養產品可調配成棒狀物、條狀物或餅乾、麵包、蛋 糕或其他烘焙食品。 在另一實施例中,本發明之營養組合物亦可以諸如膠 囊、鍵劑、丸劑、囊片及凝膠之產品形式調配。此等產品 形式一般僅含有如本文所述之基本成分,其視情況與其他 活性劑、加工助劑或其他劑型賦形劑組合。 嬰兒配方 备營養產品為嬰兒配方時,營養產品可為如上文所述之 液體或固體營養產品。本發明之液體或固體嬰兒配方產品 可具有任何適合於目標嬰兒之熱量密度’或在復原粉末實 施例時或在稀釋液體濃縮物實施例時提供該密度。舉例而 言,用於本發明之嬰兒配方實施例的最常見熱量密度—般 為至少約19 kcal/fl Oz(66〇千卡/公升),更通常為約如 kcal/fl 〇Z(675-680 千卡 / 公升)至約 25 kcai/fl 〇z(82〇 千卡 / 公 158197.doc •18· 201212828 升)’甚至更通常為約20 kcal/fl 〇z(675-680千卡/公升)至約 24kCal/fl〇z(800-810千卡/公升)。_般而言,22_24kcai/fi oz配方最常用於早產兒或低出生體重嬰兒,且2〇_2i kcal/fl 〇z(675-680至700千卡/公升)配方最常用於足月兒。 在替代實施例中,嬰兒配方為低熱量嬰兒配方且熱量 密度為約6.5 kcal/fl 〇z(220千卡/公升)。在另一實施例中, 嬰兒配方之熱量密度為約7.1 kcal/fl 〇z(24〇千卡/公升)。在 ❹一實施例中,嬰兒配方之熱量密度為約7.7 kcal/fl Oz(260 千卡/公升)。 儘管脂肪、蛋白質及碳水化合物之總濃度或量可視產品 形式(亦即粉末、即食液體或濃縮液體)及預期使用者之目 標膳食需求而變化,但該等濃度或量最通常在以下實施範 圍之一内’包括如本文所述之任何其他基本脂肪、蛋白質 及/或碳水化合物成分。 對於液體嬰兒配方產品’以營養產品之重量計,碳水化 〇 合物濃度最通常在約5%至约40%之範圍内,包括約7%至 約30%,包括約1〇%至約25% •,以營養產品之重量計脂 肪濃度最通常在約1%至約3〇%之範圍内,包括約2%至約 150/。’且亦包括約4%至約丨〇% ;且以營養產品之重量計, 蛋白質濃度最通常在約0.5%至約30%之範圍内,包括約^/。 至約15%,且亦包括約2%至約10%。 在一替代實施例中,液體嬰兒配方產品為低熱量液體嬰 兒配方且包括小於約5重量%碳水化合物、約1重量%脂肪 及約〇.5重量%蛋白質。 158197.doc -19- 201212828 液體營養產品中碳水化合物、脂肪及/或蛋白質之含量 或量亦可以如下表中所示之佔營養組合物之總卡路里百分 比形式另外或替代表徵。用於本發明之液體營養產品的此 等巨量營養素最通常調配成在下表中所述之任一熱量範圍 内(實施例A-F)(在每一數值前加上術語「約」)。 營養素佔總卡路里之% 實施例A 實施例B 實施例C 碳水化合物 0-98 2-96 10-75 蛋白質 0-98 2-96 5-70 脂肪 0-98 2-96 20-85 實施例D 實施例E 實施例F 破水化合物 30-50 25-50 25-50 蛋白質 15-35 10-30 5-30 脂肪 35-55 1-20 2-20 舉例而言,液體嬰兒配方(即食液體與濃縮液體)包括如 下彼等實施例,其中蛋白質組分可包含配方含熱量之約 7.5%至約25% ;碳水化合物組分可包含嬰兒配方總含熱量 之約35%至約50% ;且脂質組分可包含嬰兒配方總含熱量 之約30%至約60%。此等範圍僅作為實例提供,且不欲加 以限制。其他適合之範圍如下表中所示(在每一數值前加 上術語「約」)。 營養素佔總卡路里之% 實施例G 實施例Η 實施例I 碳水化合物: 20-85 30-60 35-55 脂質: 5-70 20-60 25-50 蛋白質: 2-75 5-50 7-40 158197.doc •20- 201212828 當營養產品為嬰兒配方粉時,蛋白質組分之含量為以粉 末狀營養產品之重量計約5%至約35%,包括約8%至約 12%,且包括約10%至約12% ;脂肪組分之含量為以粉末 狀營養產品之重量計約10%至約35%,包括約25%至約 • 30%,且包括約26%至約28% ;且碳水化合物組分之含量 - 為以嬰兒配方粉之重量計約30%至約85%,包括約45%至 約60%,包括約50%至約55%。 本發明之粉末狀營養產品中脂肪、碳水化合物及蛋白質 〇 之總量或濃度可視預期使用者之所選調配物及膳食或醫學 需求而顯著變化。下文闡述巨量營養素濃度之其他適合實 例。在本文中,總量或濃度係指粉末狀產品中之所有脂 肪、碳水化合物及蛋白質來源。對於嬰兒配方粉,該等總 量或濃度最通常且較佳調配成在下表中所述之任一實施範 圍内(所有數字在其前均具有「約」)。 營養素(卡路里%) 實施例A 實施例B 實施例C 碳水化合物 20-85 30-60 35-55 脂肪 5-70 20-60 25-50 蛋白質 2-75 5-50 7-40 婉豆蛋白水解物 營養產品包含豌豆蛋白水解物作為蛋白質之主要或唯一 來源。在需要低過敏性組合物(諸如低過敏性嬰兒配方)之 營養實施例中,營養組合物將包括以蛋白質組分之重量計 100%豌豆蛋白水解物。已發現與其他植物蛋白水解物(諸 158197.doc •21· 201212828 如大丑蛋白)以及牛乳蛋白相比,本發明產品中所用之豌 且蛋白水解物顯示降低之免疫反應性及改良之風味及感官 性質。舉例而言,如圖丨中所示,用於本發明營養產品中 之豌豆蛋白水解物具有降低含量之植物雌激素(亦即染料 木黃酮及大豆異黃酮,其為異黃酮)、鋁、錳及植酸鹽 (phytate),由此改良最終營養產品之營養素概況。另外, 如本文所述,豌豆蛋白水解物具有降低之抗原含量及/或 降低之免疫反應性。因此,豌豆蛋白水解物向營養產品提 供蛋白質之良好來源,該等營養產品為對牛乳蛋白及/或 大且蛋白不耐及/或過敏之個體所設計以及滿足具有吸收 障礙、消化不良及其他胃腸病狀之個體之營養需求。此等 營養產品另外具有改良之風味及乳液穩定性。 豌豆蛋白水解物可為本發明之營養產品中蛋白質之唯一 來源。或者,如下文所述之其他蛋白質來源可與豌豆蛋白 水解物組合用於產品中以提供總蛋白質。因此,豌豆蛋白 水解物提供營養產品中總蛋白質之至少5〇%,包括營養產 品中總蛋白質之約50%至約職,包括總蛋白質之:_ 至約95% ’包括總蛋白質之約7〇%至約9〇%,且亦包括總 蛋白質之約75%至約85%。如本文中所示,其他蛋白質: 源可包括部分水解、水解及/或未水解(完整)蛋白質。 尤其,已令人驚訝地發現,藉由在特定程度上控制豌豆 蛋白來源(諸如豌豆蛋白分離物或豌豆蛋白濃縮物)之: 解’可產生具有降低之抗原含量且因此具有降低之 應性的豌豆蛋白水解物,而不會不利地影響水解物之風味 158197.doc -22- 201212828 屬性。本文所述之心蛋白錢物優於先前已知之大豆蛋 白’因為其具有··⑴不可_含量之異黃酮·⑺降低之 植酸I猛及IS濃度;(3)改良之感官及風味概況;及⑷ 未經遺傳修飾。 一般而言’用於本發明營養產品中之婉豆蛋白水解物係 藉由如下產生:⑴混合具有至少㈣蛋白質(以乾物質重 量S十)之3宛丑蛋白產品盘p. Tty ^ ”欠以心成蛋白質含量至多約20%、 較佳至夕約12%之毁料;⑺水解該漿料;及⑺過濾經水解 黎料以獲得蛋白質水解物。 步驟⑴中用作原料之婉豆蛋白產品可為任何疏豆蛋白 產品’其限制條件為其蛋白質含量為以乾物質重量計至少 65/。’亦即豌丑蛋白濃縮物(蛋白質含量以乾物質重量計高 :70/。)4豌丑蛋白分離物(蛋白質含量以乾物質重量計 高於90%)。市售豌豆蛋白濃縮物及分離物為此項技術所熟 知。 〇 豌豆蛋白產品與水混合形成蛋白質含量至多約20%、較 Μ多_%之漿料。在_或多個實施例中,可在水解之 前加熱衆料以進一步變性。舉例而言,在一實施例中,該 :法進—步包括在水解之前將漿料加熱至高於6(TC之溫 度。。更適當地,可在水解之前將漿料加熱至約賊至約 90 C之溫度後持續約2分鐘 王A 20分鐘之時段。以此方 式,蛋白質有效地蠻性,日 ^ Μ此’隨後水解-般將更快速 =行。此外’藉由加熱漿料可有效地穩固水解期間之微 生物穩定性,此可持續數小時。 158197.doc -23· 201212828 一旦形成且視情況加熱後,在水解反應之前及期間,根 據此項技術中已知之方法,I佳調整衆料之溫度且維持在 約40C至約70。。,包括約5〇0C至約6〇。。。當然如此項技 術中所已知,可使用其他加熱步驟及/或溫度。 含蛋白質漿料之水解可以酶促方法或以非酶促方法進 灯。在一實施例中,含蛋白質漿料水解至經調整水解度介 於約7_0%與小於19.0%之間,包括介於約8 〇%與約16 〇% 之間,包括介於約8.0%與約135%之間,亦包括介於約 u.o%與約14.00%之間,且亦包括約14〇%;及如下文所閣 述之分子量分佈及抗原含量。在—實施例中,如此項技術 中所已知,該水解可以蛋白分解方式進行。 在另一實施例中’用一或多種非特異性作用之肽鏈内切 酶及/或肽鏈㈣酶進行水解。適合之非特異性作用之狀 鏈内切酶及/或肽鏈外切酶包括例如來源於以下菌株之 酶:麵菌屬(抑吻//叫菌株,尤其是黑麵徽(抑叩·— ⑴·#〇菌株、米麯黴^少之似)菌株或醫油麯黴 少似)菌株;或芽孢桿菌(5以㈧似)菌株,尤其 疋澱粉液化芽孢桿菌(5⑽7/似⑽⑽· 菌株、遲 缓芽孢桿菌W似)菌株、地衣芽孢桿菌(方“出似 細/簡⑷菌株或枯草芽孢桿菌(5心^以如·叫菌 株。The various embodiments of the nutritional product of the present invention may also be substantially free of any optional or selected essential ingredients or features described herein, with the proviso that the remaining nutritional product still contains all of the desired ingredients as described herein. Or feature. As used herein and unless otherwise specified, the term "substantially free of the selected nutritional product containing less than a functional amount, as the case may be selected, typically less than about 1% by weight' includes less than about 5% by weight, including less than about 0.1% by weight and also including 0% by weight of the optional or selected beauty component. The earthworm nutritional product may comprise the essential elements of the product as described herein and as described herein or otherwise applicable to nutritional product applications. Other or optionally elements are comprised of, or consist essentially of. The nutritional composition of the present invention in the form of a product comprising a ugly protein hydrolysate may be formulated and administered in any known or otherwise suitable oral form. Any solid, liquid or powder form, including combinations or variations thereof, is suitable for use herein, with the proviso that the form allows for the safe and effective oral delivery of the essential ingredients as defined herein to the individual. 158197.doc 201212828 The nutritional composition of the invention comprises a pea protein hydrolysate as described herein. The composition may comprise a cowpea protein hydrolysate The sole source of protein (ie, the soy protein hydrolysate is 1% protein component of the composition) or may comprise a combination of the cowpea protein hydrolysate and other hydrolyzed, partially hydrolyzed or unhydrolyzed (complete) proteins as described herein. The composition includes any product form comprising the essential ingredients described herein, and is safe and effective for oral administration. The nutritional composition may comprise only the essential ingredients of the materials herein, or may be used: Different product forms. The nutritional combination of the present invention (4) is a modified food product form, which is defined herein as an embodiment comprising the essential components of the present invention comprising at least one of fat, protein and carbohydrate And preferably also contains = biotin, a substance or a combination thereof. In many embodiments, the composition will comprise a pea protein hydrolysate as a sole source of protein with fat, carbohydrate hydrate, bitterin and minerals Combine to produce a hypoallergenic composition. One specific example is a hypoallergenic infant formula comprising 1% pea protein water As a source of protein, fat, carbohydrates, vitamins and minerals. Thus the 'nutrition composition of the invention may comprise a plurality of different product forms, including most of any conventional or otherwise known food forms, wherein - this is not limiting Examples include confectionery products, cereals, food seasonings (such as spreads, powders, sauces, jams, jellies, coffee creamers or sweeteners), pasta, baking or cooking substances (such as flour, fat) Bite 'breading or baking mix', cream or margarine's breading or baking mix, seasoned snacks (eg, extruded, baked, fried), drink 158I97.doc -10- 201212828 Materials (eg coffee, juice, carbonated drinks, non-carbonated drinks, tea), snacks or meal replacement bars (eg SlimfastTM sticks, EnsureTM sticks, Zone perfectTM sticks), srn〇〇thies, breakfast縠Class, milky, gummie products, salted or unsalted crispy snacks (eg flakes, shortbread, pretzels), sauces (dips), ^ Baked goods (eg biscuits, cakes, pies, pastries, bread, bagels, croutons, sauces, dry mixes (eg for ◎ muffins, biscuits, muffins) (waffles), pancakes, beverage mixes, frozen desserts (eg ice cream, popsicles, fudge bars, crushed ice, frozen yogurt)' pasta, processed meat (eg corn hot dogs, burgers, Hot dogs, sausages, red pepper sausages), pizza, pudding, seasoned or unflavored gelatin, chilled dough (eg biscuits, bread, nutry cakes), yoghurt or yogurt-based beverages, frozen yogurt Soup, vegetable-based burgers and popcorn-based snacks. The nutritional compositions of the present invention may also be formulated, for example, in the form of a capsule, lozenge, pill, caplet, gel, liquid (e.g., suspension, solution, emulsion, clear solution), powder or other particulates, and the like. Such product forms generally contain only the essential ingredients as described herein, optionally in combination with other active agents, processing aids or other dosage form excipients. The nutritional product comprises a liquid and a spray-dried powder form comprising a ready-to-eat emulsified liquid and a spray-dried reconstitutable powder which can be diluted with water or other aqueous liquid to form a nutrient solution prior to application. In particular, the nutritional composition may be a human milk fortifier for premature infants who are intolerant to a formula based on cow's milk protein or soy protein, for infants allergic to cow's milk protein 158197.doc •11·201212828 or soy protein. Human milk fortifier for preterm infant formula for premature infants who are intolerant to formula based on cow's milk protein or soy protein, or for preterm infant formula for premature infants allergic to cow's milk protein or soy protein, for milk-based protein Or infant formula formulated with soy protein, 'infant formula for infants allergic to cow's milk protein or soy protein, for hypoallergenic formulas for infants with general food allergies, for cow milk protein based or soy A formula for infants who are intolerant to infant formula (f〇U〇W_on infant formulas) for larger infant formulas for infants who are allergic to formula based on cow's milk protein or soy protein for use in cow milk protein or soy A formula for infants who are intolerant to protein for more than 6 months, for 6 months allergic to formula based on cow's milk protein or soy protein A baby formula for infants and adults who are not resistant to cow's milk protein or soy protein, used for nutritional beverages for children and adults who are allergic to milk protein based on cow's milk protein or soy protein. a nutritional beverage for elderly patients who are intolerant to milk protein based on cow's milk protein or soy protein, a nutritional beverage for a patient who is allergic to milk protein based on cow's milk protein or soy protein, for use in milk protein based or A sports nutrition drink for people who are intolerant to soy protein, used in sports nutrition drinks for people who are allergic to milk protein based on cow's milk protein or large ugly protein, using sports nutrition drinks with hydrolyzed protein with improved sensory properties. A nutrient stick for the improvement of organoleptic properties of hydrolyzed proteins, which is used as a semi-element for the nutritional needs of patients with dysfunction and other gastrointestinal conditions - formulas, sticks, liquid or powdery protein supplements requiring hydrolysis of proteins Based on the hydrolysis of eggs 158197.doc -12- 201 212828 Other food forms of white matter. When the nutritional composition of the present invention is formulated into a dietary product form, it may provide the individual with a single source of nutrients or a source of supplemental nutrients. In this context, the sole source of nutrition may be administered one or more times per day to provide the individual with daily or All or substantially all of its fat, protein, - carbohydrate, mineral and vitamin requirements are expected during the administration. Supplementary sources of nutrition are defined herein as dietary sources that do not provide individuals with a potential source of unique nutrition. Liquid beverages Ο Secret Liquid beverages include concentrated and ready-to-feed liquids. Non-limiting examples of liquid product forms suitable for use herein include liquid infant formulas (including ready-to-eat formulations and concentrated formulations), liquid infant formulas (including ready-to-eat formulations and concentrated formulations), liquid human milk fortifiers (including ready-to-feed blending) And concentrated formulations), snacks and meal replacement products, hot or cold drinks, carbonated or non-carbonated beverages, juice or other acidified beverages, shakes, coffee, tea, enteral feeding compositions, and the like. These liquid compositions are most often formulated as a suspension or emulsion, but may be formulated in any other suitable form, such as clear liquid solutions, liquid gels and the like. The nutrient solution of the present invention can be used as a nutritious drink for premature infants, infants, young children, children and adults (including elderly patients) who are intolerant or allergic to milk based on cow's milk protein and/or protein. Liquid beverages can also be used as a semi-element formula for the nutritional needs of patients with malabsorption, dyspepsia and other gastrointestinal conditions. A. Nutrient Emulsion The nutritional emulsion contains the tofu protein hydrolysate as the main or only source of protein and is an aqueous emulsion containing fat and carbohydrates. Such emulsions are in the form of flowable or potable liquids at about rc to about 2 rc and are typically in the form of oil-in-water, water-in-oil or complex aqueous emulsions, but such emulsions most often have a continuous aqueous phase and a discontinuous oil phase. In the form of an oil-in-water emulsion. The nutritional emulsion can be and is usually stable. The nutritional emulsion typically contains up to about 95% by weight water, including from about 5% to about 95%, including from about 60% to about 9%, and also from about 7% to about 8, based on the weight of the nutritional emulsion. 5 % water. The nutritional emulsion can be formulated to contain sufficient amounts and amounts of nutrients to provide a unique, primary or supplemental source of nutrition, or to provide a specialized nutritional emulsion for an individual suffering from a particular disease or condition. For example, it is possible to produce a nutritional emulsion for use as an infant, a larger infant and toddler formula, an adult formula and the like for use in a user who is intolerant or allergic to a nutritional protein based on cow's milk protein and/or soy protein. In another embodiment, the emulsion can be used as a hypoallergenic nutritional emulsion for individuals with general food allergies. Thus, such nutritional emulsions can have a variety of product densities, but most typically have a density greater than about 丨〇3 g/ml, including greater than about 1.04 g/ml, including greater than about Low g/ml, including about ^6^g/ml. Up to about i.12 g/ml 'and also includes from about 〇85 g/mi to about i 1〇g/ml ° The nutritional emulsion can have a caloric density suitable for the end user's nutritional needs 'but in most cases' The emulsion comprises from about 1 〇〇 kcal / 24 〇mi to about 500 kcal / 240 ml ' including from about 150 kcal / 240 ml to about 350 kcal / 240 m b and also includes from about 200 kcal / 240 ml to about 320 kcal / 240 m In an alternate embodiment, the 'nutrient emulsion is a low calorie nutritional emulsion and the heat is 158197.doc •14·201212828 and the density is about 6.5 kcal/fl oz (52.8 kcal/24 〇 ml). In another embodiment, the nutritional emulsion has a caloric density of about kcal/fl 〇z (57 6 keal/24() m1). In one embodiment, the nutritional emulsion has a caloric density of about 77 kcal/fl 〇ζ (62·4 kcal/240 ml). • The pH of the nutritional emulsion may range from about 3.5 to about 8, but is most preferably in the range of from about -4.5 to about 7.5, including from about 5.5 to about 7.3, including from about 62 to about 7.2. 0 The serving size of the nutritional lotion may vary depending on a number of variables, but typical serving sizes range from about 1 〇〇ml to about 3 〇〇W, including from about 150 ml to about 250 ml, and include about 19 to, for example, about 24 〇mi. In one embodiment, the nutritional emulsion can be used as a human milk fortifier. In such embodiments, a typical single serving size comprises about 5. 〇 ml. B. Substantially Clarifying the Liquid Beverage The substantially clarified nutrient solution of the present invention is a dilute liquid comprising at least a protein and a carbohydrate as discussed below, at least a portion of which is a cowpea protein hydrolysate. It is substantially clarified that the nutrient solution is substantially free of fat; that is, the liquid does not contain fat, except that it is inherently (four) or added at a low concentration to help make the fat of the liquid. As used herein, the term "free of fat" means that the liquid typically contains less than 1 (%) by weight of the nutrient solution, more typically less than 0.5% and more typically less than 0.1%, including 〇% fat. These are substantially clarifying that the nutrient solution is a flowable or drinkable liquid at about to about 25 < t. The clarifying nutrient solution can be and is generally stable in storage. The nutrient solution typically contains up to about 95% by weight of 158I97.doc -15-201212828 water, including from about 50% to about 95%, also including from about 60% to about 90%, and also from about 5% by weight of the nutrient solution. It includes from about 70% to about 85% water. Substantially clarifying the nutrient solution can be formulated to contain sufficient amounts and amounts of nutrients to provide a supplemental source of nutrients, or to provide a specialized nutrient solution for an individual suffering from a particular disease or condition. For example, in one embodiment, the substantially clarified nutrient solution is a sports drink for use by a user who is intolerant or allergic to a nutritional protein based on cow's milk protein and/or soy protein. In another embodiment, the substantially clarified nutrient solution is a sports drink comprising hydrolyzed protein having improved organoleptic properties. Thus, such substantially clarified nutrient solutions can have a variety of product densities' but most typically have a density greater than about 1.040 g/ml, including 1.06 g/ml to 1.12 g/ml, and also include from about 1.085 g/ml to about 1 〇. g/ml. Substantially clarifying that the nutrient solution may have a heat rating that is suitable for the end user's nutritional needs 'but in most cases' the liquid contains from about 9 〇 kcal / 240 ml to about 500 kcal / 240 ml, including about 150 kcal / 240 The ml to about 350 kcal/240 ml' includes from about i8〇kcal/240 ml to about 350 kcal/240 ml and also includes from about 250 kcal/240 ml to about 320 kcal/240 ml. In other embodiments, the 'substantially clarified nutrient solution comprises from about 9 〇 kcal / 48 〇 ml to about 500 kcal / 480 m b including about 150 kcal / 480 ml to about 350 kcal / 480 m b and also includes about 25 〇 kcai /48〇ml to about 32〇kcal/48〇. The pH of the clarified nutrient solution is in the range of from about 2.8 to about 4.6, including from about 2.9 to about 4.2, and also from about 3.1 to about 3.9. The amount of nutrient solution may vary substantially depending on a number of variables, but typical sizes range from about 1 〇〇ml to about 591 ml, including from about 150 mi to about 25 〇m and include about 19 claws.丨到约24〇爪卜实158197, doc -16· 201212828 Some specific parts of the clarifying nutrient solution include 240 ml (8" plates), 296 ml (10 plates) and 480 ml (16 ounces). Nutritional Powder The nutritional powder comprises a cowpea protein hydrolysate as the primary or sole source of protein and is typically in the form of a flowable or substantially flowable particulate composition or at least a particulate composition. Particularly suitable product forms include spray dried, coalesced or dry blended powder compositions. The compositions can be easily taken and measured with a spoon or similar device, wherein the composition can be readily reconstituted by the intended user with a suitable aqueous liquid (usually water) to form a nutrient for immediate oral or enteral use. Formulation. As used herein, "immediately" use generally means within about 48 hours' most commonly within about 24 hours, preferably immediately after recovery. The nutritional powder can be formulated to contain sufficient amounts and amounts of nutrients to provide a unique, primary or supplemental source of nutrition, or to provide a specialized nutritional powder for individuals suffering from a particular disease or condition. In a particular embodiment, the nutritional powder is formulated for use in individuals who are intolerant or oversensitive to dairy protein and/or soy protein based nutritional products. In another embodiment, the emulsion can be used as a hypoallergenic nutritional emulsion for individuals with general food allergies. The nutritional powder can be reconstituted with water prior to use to a caloric density suitable for the end user's nutritional needs, but in most cases the powder is reconstituted with water to form from about 100 kcal/240 ml to about 500 kcal/240 ml, including about 150 kcal. /240 ml to about 350 kcal/240 ml and also includes a composition of from about 200 kcal/240 ml to about 320 kcal/240 ml. In an alternate embodiment, the nutritional powder can be reconstituted with water to form a low calorie composition having a caloric density of about 52.8 kcal/240 ml. In another embodiment, the caloric density of the 158197.doc -17-201212828 powder is about 57.6 kcal/240 m. In the embodiment, the caloric density of the nutrient powder is about 62.4 kcal/240 m. The amount of nutrient solution may vary depending on a number of variables, but a typical portion size ranges from about 1 〇〇ml to about 3 〇〇ml, including from about WO 〇11 to about 25 〇1111' including about 19 () 1111 to about 24 () 1111. In a special implementation, the nutrient solution can be used as a human milk fortifier. In such embodiments, a typical single serving size comprises about 0.9 grams of powder per about (10) Torr to about 3 Torr of water. Other Gu Zhao Nutritional Differences One, nutritional products can be in the form of solid nutrition bars and food. For example, nutritional products can be formulated into sticks, sticks or biscuits, bread, cakes or other baked goods. In another embodiment, the nutritional compositions of the present invention may also be formulated in the form of a product such as a capsule, a key, a pill, a caplet, and a gel. These product forms generally contain only the essential ingredients as described herein, optionally in combination with other active agents, processing aids or other dosage form excipients. Infant Formula When the nutritional product is an infant formula, the nutritional product may be a liquid or solid nutritional product as described above. The liquid or solid infant formula of the present invention may have any caloric density suitable for the target infant' or provide this density when reconstituting the powder embodiment or in diluting the liquid concentrate embodiment. For example, the most common caloric density for an infant formula embodiment of the invention is generally at least about 19 kcal/fl Oz (66 〇 kcal/liter), more typically about kcal/fl 〇Z (675- 680 kcal / liter) to about 25 kcai / fl 〇z (82 〇 kcal / public 158197.doc • 18 · 201212828 liter) 'even more usually about 20 kcal / fl 〇 z (675-680 kcal / liter ) to about 24kCal/fl〇z (800-810 kcal / liter). In general, the 22_24kcai/fi oz formula is most commonly used in premature or low birth weight infants, and 2〇_2i kcal/fl 〇z (675-680 to 700 kcal/litre) formula is most commonly used in term infants. In an alternate embodiment, the infant formula is a low calorie infant formula and has a caloric density of about 6.5 kcal/fl 〇z (220 kcal/liter). In another embodiment, the infant formula has a caloric density of about 7.1 kcal/fl 〇z (24 〇 kcal/liter). In one embodiment, the infant formula has a caloric density of about 7.7 kcal/fl Oz (260 kcal/liter). While the total concentration or amount of fat, protein, and carbohydrate may vary depending on the product form (ie, powder, ready-to-feed liquid, or concentrated liquid) and the intended dietary needs of the intended user, such concentrations or amounts are most commonly in the following ranges of practice. One includes 'any other essential fat, protein and/or carbohydrate component as described herein. For liquid infant formulas, the concentration of the carbohydrate hydrate is most typically in the range of from about 5% to about 40%, including from about 7% to about 30%, including from about 1% to about 25, by weight of the nutritional product. %• The fat concentration, most typically from about 1% to about 3% by weight of the nutritional product, includes from about 2% to about 150%. And also includes from about 4% to about 丨〇%; and the protein concentration is most typically in the range of from about 0.5% to about 30% by weight of the nutritional product, including about +/-. Up to about 15%, and also includes from about 2% to about 10%. In an alternate embodiment, the liquid infant formula is a low calorie liquid infant formula and comprises less than about 5% by weight carbohydrate, about 1% by weight fat, and about 5% by weight protein. 158197.doc -19- 201212828 The amount or amount of carbohydrate, fat and/or protein in the liquid nutritional product may also be additionally or alternatively characterized as a percentage of the total calorie of the nutritional composition as shown in the following table. These macronutrients for the liquid nutritional product of the present invention are most commonly formulated into any of the calories described in the following table (Examples A-F) (the term "about" is added before each value). Nutrient as a percentage of total calories Example A Example B Example C Carbohydrate 0-98 2-96 10-75 Protein 0-98 2-96 5-70 Fat 0-98 2-96 20-85 Example D Implementation Example E Example F Water-breaking compound 30-50 25-50 25-50 Protein 15-35 10-30 5-30 Fat 35-55 1-20 2-20 For example, liquid infant formula (ready liquid and concentrated liquid) Including the following embodiments, wherein the protein component may comprise from about 7.5% to about 25% of the caloric content of the formulation; the carbohydrate component may comprise from about 35% to about 50% of the total caloric content of the infant formula; and the lipid component may Contains from about 30% to about 60% of the total calories of the infant formula. These ranges are provided as examples only and are not intended to be limiting. Other suitable ranges are shown in the table below (the term "about" is added before each value). Nutrients accounted for % of total calories. Example G Example 实施 Example I Carbohydrate: 20-85 30-60 35-55 Lipid: 5-70 20-60 25-50 Protein: 2-75 5-50 7-40 158197 .doc •20- 201212828 When the nutritional product is an infant formula, the protein component is present in an amount of from about 5% to about 35% by weight of the powdered nutritional product, including from about 8% to about 12%, and includes about 10 % to about 12%; the fat component is present in an amount of from about 10% to about 35% by weight of the powdered nutritional product, including from about 25% to about 30%, and including from about 26% to about 28%; and carbon water The amount of the compound component is from about 30% to about 85% by weight of the infant formula, including from about 45% to about 60%, including from about 50% to about 55%. The total amount or concentration of fat, carbohydrate and protein mash in the powdered nutritional product of the present invention may vary significantly depending on the intended formulation of the user and the dietary or medical need. Other suitable examples of macronutrient concentrations are set out below. As used herein, the total amount or concentration refers to all sources of fat, carbohydrate and protein in a powdered product. For infant formulas, these totals or concentrations are most commonly and preferably formulated to fall within any of the ranges set forth in the table below (all numbers have "about" before them). Nutrients (calories %) Example A Example B Example C Carbohydrate 20-85 30-60 35-55 Fat 5-70 20-60 25-50 Protein 2-75 5-50 7-40 Cowpea Protein Hydrolysate The nutritional product contains pea protein hydrolysate as the primary or sole source of protein. In a nutritional embodiment where a hypoallergenic composition, such as a hypoallergenic infant formula, is desired, the nutritional composition will comprise 100% pea protein hydrolysate by weight of the protein component. It has been found that the protein and hydrolysate used in the products of the present invention exhibit reduced immunoreactivity and improved flavor compared to other vegetable protein hydrolysates (such as 158197.doc • 21·201212828 such as large ugly protein) and cow's milk protein. Sensory nature. For example, as shown in FIG. ,, the pea protein hydrolysate used in the nutritional product of the present invention has a reduced content of phytoestrogens (ie, genistein and soy isoflavones, which are isoflavones), aluminum, manganese. And phytate, thereby improving the nutrient profile of the final nutritional product. Additionally, as described herein, the pea protein hydrolysate has a reduced antigen content and/or reduced immunoreactivity. Thus, pea protein hydrolysate provides a good source of protein to nutritional products designed for individuals with bovine milk protein and/or large and protein intolerant and/or allergic, as well as for malabsorption, dyspepsia and other gastrointestinal disorders. The nutritional needs of individuals with symptoms. These nutritional products additionally have improved flavor and emulsion stability. The pea protein hydrolysate can be the sole source of protein in the nutritional product of the present invention. Alternatively, other protein sources, as described below, can be used in the product in combination with the pea protein hydrolysate to provide total protein. Thus, the pea protein hydrolysate provides at least 5% of the total protein in the nutritional product, including about 50% of the total protein in the nutritional product to the co-worker, including total protein: _ to about 95% 'including about 7 总 of total protein % to about 9%, and also includes from about 75% to about 85% of total protein. As shown herein, other proteins: the source may include partially hydrolyzed, hydrolyzed, and/or unhydrolyzed (intact) proteins. In particular, it has been surprisingly found that by controlling the pea protein source (such as the pea protein isolate or the pea protein concentrate) to a certain extent: the solution can produce a reduced antigen content and thus a reduced solubility. Pea protein hydrolysate without adversely affecting the flavor of the hydrolysate 158197.doc -22- 201212828 Properties. The heart protein described herein is superior to the previously known soy protein 'because it has (1) non-isof content of isoflavones (7) reduced phytic acid I and IS concentration; (3) improved sensory and flavor profile; And (4) not genetically modified. In general, the cowpea protein hydrolysate used in the nutritional product of the present invention is produced by: (1) mixing a 3 ugly protein product tray having at least (four) protein (at a dry matter weight of S 10) p. Tty ^ owed a protein having a protein content of up to about 20%, preferably up to about 12%; (7) hydrolyzing the slurry; and (7) filtering the hydrolyzed material to obtain a protein hydrolysate. The asparagus protein used as a raw material in the step (1) The product may be any gluten protein product 'with the proviso that its protein content is at least 65 per dry weight.' </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> Ugly protein isolate (protein content greater than 90% by weight of dry matter). Commercially available pea protein concentrates and isolates are well known in the art. The pea protein product is mixed with water to form a protein content of up to about 20%. More than _% of the slurry. In _ or more embodiments, the mass may be heated for further denaturation prior to hydrolysis. For example, in one embodiment, the method includes: adding the slurry prior to hydrolysis Heated to above 6 (TC temperature More suitably, the slurry may be heated to a temperature of about thief to about 90 C before hydrolysis for about 2 minutes for a period of about 20 minutes. In this way, the protein is effectively savage, day ^ Μ 'Subsequent hydrolysis will generally be faster = OK. In addition, by heating the slurry, the stability of the microorganism during hydrolysis can be effectively stabilized, which can last for several hours. 158197.doc -23· 201212828 Once formed and heated as appropriate, Before and during the hydrolysis reaction, I preferably adjust the temperature of the batch and maintain it at a temperature of from about 40 C to about 70, including from about 5 〇 0 C to about 6 Torr, according to methods known in the art. It is known that other heating steps and/or temperatures can be used. The hydrolysis of the protein-containing slurry can be carried out enzymatically or non-enzymatically. In one embodiment, the protein-containing slurry is hydrolyzed to an adjusted degree of hydrolysis. Between about 7_0% and less than 19.0%, including between about 8% and about 16%, including between about 8.0% and about 135%, including between about uo% and about 14.00 Between %, and also includes about 14%; and as explained below Amount distribution and antigen content. In an embodiment, as is known in the art, the hydrolysis can be carried out in a proteolytic manner. In another embodiment, 'one or more non-specifically acting endopeptidases and / or peptide chain (4) enzyme hydrolysis. Suitable non-specific endostases and / or exonucleases include, for example, enzymes derived from the following strains: Genus (suppression / / called strains, especially It is a black-faced emblem (suppressive--(1)·#〇 strain, Aspergillus oryzae) or a strain of Bacillus (5), especially Bacillus licheniformis ( 5(10)7/like (10)(10)· strain, Bacillus lentus-like strain, Bacillus licheniformis (fang "like fine/simple (4) strain or Bacillus subtilis (5 heart ^ as called strain).
用於水解步驟(2)中之其他適合之肽鏈内切酶包括例 如:⑴麩胺醯基肽鏈内切酶(EC 3 4 21 19) ; (ii)離胺醯基 肽鏈内切酶(EC 3.4.21.50) ; (iii)白胺醯基肽鏈内切酶(EC 158197.doc 24· 201212828Other suitable endopeptidases for use in the hydrolysis step (2) include, for example: (1) glutamine thiol endopeptidase (EC 3 4 21 19); (ii) amidoxime endopeptidase (EC 3.4.21.50); (iii) leucine thiol endopeptidase (EC 158197.doc 24· 201212828
3.4.21.57) ; (iv)甘胺醯基肽鏈内切酶(EC 3.4·22·25) ; (ν)脯 胺醯基肽鏈内切酶(EC 3.4.21.26) ; (vi)胰蛋白酶(EC 3.4.21.4) 或騰蛋白酶樣(離胺酸/精胺酸特異性)肽鏈内切 酶;或(vii)肽基-Asp金屬肽鏈内切酶(EC 3.4.24.33)。其他 適合之肽鏈外切酶包括例如羧肽酶,諸如:⑴脯胺酸羧肽 Ο Ο 酶(EC 3.4.16.2) ; (ii)羧肽酶 a(EC 3.4.17.1) ; (iii)羧肽酶 B(EC 3.4.17.2) ; (iv)羧肽酶 c(EC 3.4.16.5) ; (ν)羧肽酶 D(EC 3.4.16.6) ; (vi)離胺酸(精胺酸)羧肽酶(Ec 3.4_ 1 7.3) ; (vii)甘胺酸竣肽酶(ec 3·4.17_4) ; (viii)丙胺酸 叛肽酶(EC 3.4.17.6) ; (ix)麩胺酸羧肽酶(Ec 3.4.17.11);3.4.21.57); (iv) Glycosyl endopeptidase (EC 3.4·22·25); (ν) amidoxime endopeptidase (EC 3.4.21.26); (vi) Trypsin (EC 3.4.21.4) or a protease (like lysine/arginine specific) endopeptidase; or (vii) peptidyl-Asp metalloendase (EC 3.4.24.33). Other suitable endopeptidases include, for example, carboxypeptidases such as: (1) valine carboxypeptidase (EC 3.4.16.2); (ii) carboxypeptidase a (EC 3.4.17.1); (iii) carboxy Peptidase B (EC 3.4.17.2); (iv) carboxypeptidase c (EC 3.4.16.5); (v) carboxypeptidase D (EC 3.4.16.6); (vi) lysine (arginine) carboxylate Peptidase (Ec 3.4_ 1 7.3); (vii) glycinate peptidase (ec 3·4.17_4); (viii) alanine tickopeptidase (EC 3.4.17.6); (ix) glutamic acid carboxypeptide Enzyme (Ec 3.4.17.11);
(X)肽基-二肽酶 A(EC 3.4_15_1);或(Xi)肽基-二肽酶(EC 3.4.15.5) 。 添加至漿料中以獲得婉豆蛋白水解物之所需特性之酶的 精確量將視諸如以下之特徵而變化:(1)所用之酶或酶系 統;(2)所需最終水解度;及/或(3)所需最終分子量分佈。 因為此等特徵為已知參數,所以一般技術者可容易地確定 達成最終豌豆蛋白水解物之所需特性的適當條件。通常, 酶或酶系統以在漿料中每35 kg蛋白質約1〇叫至約测 g、包括35 mg至約700 g之範圍内的量添加至漿料中。 水解反應通常將進行所需時間長度以獲得所需水解度及/ 或所需分子量概況。通常’水解反應將持續約叫鐘:約 24小時之時段,包括約财鐘至約24()分鐘,|亦包㈣ 45分鐘至約120分鐘。此外,水解反應所需之確切時間長 度將視如上文所述之特定特徵而定,但可容易由熟習:匕項 158197.doc -25· 201212828 技術者確定。 在其他實施例中,含蛋白質漿料可使用非酶促方法水 解,諸如藉由化學及/或機械(物理)水解。該技術為此項技 術中亦熟知之技術。 豌旦蛋白物質水解至所需程度後,例如藉由使酶或酶系 統失活或藉由其他習知方法使水解反應停止。在一實施例 中,错由熱處理使酶或酶系統失活。在最終豌豆蛋白水解 物之PH值將會相對高的情況下,此失活尤其充分適合。 /根據慣例,可適當地藉由使培育漿料之溫度增加至酶變 得失活之溫度(例如增加至高於約7〇<t且適當地為約 之溫度)來使酶製劑失活。 或者,可藉由適合之酸處理使酶失活。在最終豌豆蛋白 2解物之pH值將會相對低的情況下,此失活尤其充分適 °舉例而δ,可藉由使漿料之pH值降低至酶變得失活之 值(例如低於約4.0)來使酶失活。 視情況,在已淬滅水解反應後,製備方法可進一步包含 在介於約50°C與約70。(:之間的溫度用活性碳處理漿料一段 適合時間,例如相對於乾物質含量計算,活性碳之量一般 對應於約1 /〇至約5%碳之間。此視情況之碳步驟可改良最 終豌且蛋白水解物之顏色且亦進一步降低豌豆蛋白水解物 之異味。 最後,利用適合之習知過濾法過濾水解漿料以獲得豌豆 蛋白水解物。舉例而言,在一個實施例中,藉由在介於 C 〃、70 C之間的溫度下奈米過濾及/或藉由蒸發進行過 158197.doc •26· 201212828 隨後收集保留物作為碗豆蛋白水解物溶液。藉助於太 未過慮,可藉由適當選擇膜來進行除鹽。蒸發 = 前獲得高含量乾物質於濃縮物中之優點。… 在―㈣U施例中’藉由上文概述之過濾、製程獲得之 豌旦蛋白水解物進行第二過濾製 奴人 i,愿表程,諸如超濾製程,以提 供適口用於半要素及相關營養產品 ^ Λ <77于篁分佈。超濾單 為中工纖維膜’例如以商標咖⑽購自Amic〇n o corporation之單元及/或以商標—購自R〇micon C〇—n之單元’具有所需標稱截止(⑽禮)分子量 值。中空纖維膜裝填於遽筒中以允許流體通過其中,適告 小分子穿過該膜’水解物流出率視穿過膜之壓降而定,且 該等小分子以溶液帶至收集區;溶液之剩餘部分含有較高 分子量蛋白質水解物’直接沿纖維管通過,視需要再循環 至水解反應器或至分開之收集區。 此外’此視情況之第二過濾步驟可降低豌豆蛋白水解物 〇中所得植酸鹽含量。特別地’碗豆蛋白水解物中之植酸鹽 含量降低至少約1〇% ’包括至少約15%,包括至少約 20% ’包括至少約25%,包括至少約齋〇 ’包括至少約 35%,包括至少約40%,及包括至少約鄉或桃以上。 使用上述方法製備詩本文所述之營養產A中的婉豆蛋 白水解物且其具有以下特性。根據以上方法製得之例示性 豌丑蛋白水解物包括分子量分佈為小於5%之蛋白質大於5 kD的婉豆蛋白水解物。 根據上述方法所產生之豌豆蛋白水解物另外具有降低之 158197.doc -27- 201212828 免疫反應性。在一實施例中,使用抑制ELISA(酶聯免疫吸 附檢定)分析豌豆蛋白水解物之免疫反應性且以每一定量 蛋白質之免疫活性抗原量表示。舉例而言,根據本文所述 之方法製備的1公克婉豆蛋白水解物通常將包括小於約 15,000 免疫活性豌豆抗原,包括小於約1〇〇〇〇吨免疫 活性豌豆抗原,包括小於約7,500盹免疫活性豌豆抗原, 包括小於約5,000 pg免疫活性婉豆抗原,包括小於約(X) Peptidyl-dipeptidase A (EC 3.4_15_1); or (Xi) Peptidyl-dipeptidase (EC 3.4.15.5). The exact amount of enzyme added to the slurry to obtain the desired properties of the cowpea protein hydrolysate will vary depending on such characteristics as: (1) the enzyme or enzyme system used; (2) the desired degree of final hydrolysis; / or (3) the desired final molecular weight distribution. Because these features are known parameters, one of ordinary skill in the art can readily determine the appropriate conditions for achieving the desired properties of the final pea protein hydrolysate. Typically, the enzyme or enzyme system is added to the slurry in an amount ranging from about 1 Torr to about g, including 35 mg to about 700 g, per 35 kg of protein in the slurry. The hydrolysis reaction will typically be carried out for the desired length of time to achieve the desired degree of hydrolysis and/or the desired molecular weight profile. Typically, the hydrolysis reaction will last for about 24 hours: from about 24 hours to about 24 minutes, and from 4 minutes to about 120 minutes. In addition, the exact length of time required for the hydrolysis reaction will depend on the particular characteristics described above, but can be readily determined by those skilled in the art: 158197.doc -25 20121228. In other embodiments, the protein-containing slurry can be hydrolyzed using a non-enzymatic method, such as by chemical and/or mechanical (physical) hydrolysis. This technique is also well known in the art. After the pea protein material has been hydrolyzed to the desired extent, the hydrolysis reaction is stopped, for example, by inactivating the enzyme or enzyme system or by other conventional methods. In one embodiment, the heat is used to inactivate the enzyme or enzyme system. This inactivation is particularly well suited where the pH of the final pea protein hydrolysate will be relatively high. / In accordance with convention, the enzyme preparation may be suitably inactivated by increasing the temperature of the incubation slurry to a temperature at which the enzyme becomes inactive (e.g., increased to a temperature above about 7 Torr < t and suitably about). Alternatively, the enzyme can be inactivated by a suitable acid treatment. In the case where the pH of the final pea protein 2 solution will be relatively low, this inactivation is particularly adequate for example δ, by lowering the pH of the slurry to a value at which the enzyme becomes inactive (eg, low) The enzyme was inactivated at about 4.0). Optionally, the preparation process may further comprise between about 50 ° C and about 70 after the hydrolysis reaction has been quenched. (The temperature between the treatments is treated with activated carbon for a suitable period of time, for example, relative to the dry matter content, the amount of activated carbon generally corresponds to between about 1 / Torr to about 5% carbon. This carbon step may optionally be The color of the final phacoprotein hydrolysate is modified and the odor of the pea protein hydrolysate is further reduced. Finally, the hydrolyzed slurry is filtered using a suitable conventional filtration method to obtain a pea protein hydrolysate. For example, in one embodiment, By using nanofiltration at a temperature between C 〃, 70 C and/or by evaporation, 158197.doc •26·201212828 then collects the retentate as a solution of the soy protein hydrolysate. Desalting can be carried out by appropriately selecting the membrane. Evaporation = the advantage of obtaining a high content of dry matter in the concentrate before.... In the "(IV) U example, 'the hydrolysis of the phage protein obtained by the filtration and the process outlined above" The second filter is made by the slave, i wish to watch the process, such as the ultrafiltration process, to provide a palatable use for the semi-element and related nutritional products ^ Λ < 77 in the 篁 distribution. The ultrafiltration single is a medium fiber membrane ' The trademark coffee (10) is purchased from the unit of Amic〇no corporation and/or the unit of the trademark - purchased from R〇micon C〇-n has the required molecular cutoff value ((10)). The hollow fiber membrane is packed in the cylinder to Allowing fluid to pass therethrough, advising small molecules passing through the membrane 'The rate of hydrolysis outflow depends on the pressure drop across the membrane, and the small molecules are brought to the collection zone as a solution; the remainder of the solution contains higher molecular weight protein hydrolysis The material 'passes directly along the fiber tube and is recycled to the hydrolysis reactor or to separate collection zones as needed. Furthermore, this second filtration step can reduce the phytate content obtained in the pea protein hydrolysate. In particular The phytate content of the pea protein hydrolysate is reduced by at least about 1%%, including at least about 15%, including at least about 20% 'including at least about 25%, including at least about 〇' including at least about 35%, including at least About 40%, and including at least about township or peach. The cowpea protein hydrolysate in the nutraceutical A described herein is prepared using the above method and has the following characteristics. Exemplary peas prepared according to the above method The ugly protein hydrolysate comprises a cowpea protein hydrolysate having a molecular weight distribution of less than 5% of protein greater than 5 kD. The pea protein hydrolysate produced according to the above method additionally has a reduced 158197.doc -27-201212828 immunoreactivity. In the examples, the immunoreactivity of the pea protein hydrolysate is analyzed using inhibition ELISA (enzyme-linked immunosorbent assay) and is expressed as the amount of immunologically active antigen per quantitative protein. For example, 1 gram prepared according to the methods described herein. The cowpea protein hydrolysate will typically comprise less than about 15,000 immunologically active pea antigens, including less than about 1 ton of immunologically active pea antigen, including less than about 7,500 angstroms of immunologically active pea antigen, including less than about 5,000 pg of immunologically active cowpea antigen. , including less than about
Kg免疫活性跳豆抗原’包括小於約5〇〇叫免疫活性婉豆抗 原,包括小於約250盹免疫活性豌豆抗原,包括小於約 1〇〇 Kg免疫活性豌豆抗原,包括小於約5〇μ§免疫活性豌豆 抗原,且包括豌豆蛋白水解物實質上不含免疫活性豌豆抗 原。 另外,藉由上述方法所產生之豌豆蛋白水解物之免疫反 應指數小於2.5 l〇gIRI單位,包括小於2 4i〇gIRl單位,包 括小於2.3 1〇§1111單位,包括小於221〇§1111單位,包括小 於2·1 log IRI單位,且亦包括小於2 〇 1〇g IRI單位。 蛋白質 本發月之營養產品可視情況進一步包含除婉豆蛋白水解 物外其他來源之蛋白質。適用於口服營養產品且與該等產 品之基本要素及特徵相容的任何其他蛋白質來源適合與豌 豆蛋白水解物组合使用。 當用於液體飲❹時,以營養液飲料之重量計,營養液 飲料中之總蛋白f濃度可在約0.5%至約40%之範圍内,包 括约0.5%至約3〇%,包括約1%至約15%,且亦包括約】%至 158197.doc -28 - 201212828 約跳’且亦包括約1%至约7%。在八 養乳液中蛋白質之含量為以營養乳液之重=施例中,營 當用於固體營養產品(諸如營養粉族約6.0%。 中時,以固體營養產品之重量計,固體營養養棒其他食品) 白質道;f可* Α π , 賓"產 中之總蛋 白賢展度了在約1〇%至約5〇%之範圍内 文 50%,且亦包括的〗Λ()/ s从 包括約10%至約Kg immunologically active hopomizing antigen 'includes less than about 5 免疫 called immunologically active cowpea antigen, including less than about 250 盹 immunologically active pea antigen, including less than about 1 〇〇Kg of immunologically active pea antigen, including less than about 5 〇μ§ immunity The active pea antigen, and including the pea protein hydrolysate, is substantially free of immunologically active pea antigen. In addition, the pea protein hydrolysate produced by the above method has an immune response index of less than 2.5 l〇gIRI units, including less than 2 4 i〇gIR1 units, including less than 2.3 1〇§1111 units, including less than 221〇§1111 units, including Less than 2·1 log IRI units, and also includes less than 2 〇1〇g IRI units. Protein The nutritional product of this month may further contain proteins other than cowpea protein hydrolysate. Any other source of protein suitable for oral nutritional products and compatible with the essential elements and characteristics of such products is suitable for use in combination with the pea protein hydrolysate. When used for liquid beverages, the total protein f concentration in the nutrient solution beverage may range from about 0.5% to about 40%, including from about 0.5% to about 3%, including about the weight of the nutrient solution beverage. 1% to about 15%, and also includes about 5% to 158197.doc -28 - 201212828 and also include about 1% to about 7%. The content of protein in the eight-nuclear emulsion is the weight of the nutritional emulsion. In the example, when used as a solid nutritional product (such as about 6.0% in the nutritional powder family), the solid nutritional nutrition is based on the weight of the solid nutritional product. Other foods) White matter; f can * Α π , 宾 · "The total protein production in the production range of about 1% to about 5% of the text within 50% of the text, and also included Λ ) () / s from about 10% to about
刀匕栝約10%至約3〇〇/〇。在_ A 霧乾燥營養粉中蛋白質含量 纟%例中,喷 ❹ Ο 計約19%。 3量為以噴霧乾燥營養粉之重量 適合用於營養產品_ 實例包括心或其來源的非限制性 貫例水解、部分水解或非水解蛋自質Μ自質來源, 其可來源於任何已知或另外適合 ’、 <求源,諸如乳(例如酪 蛋白、乳清)、動物(例如肉類、魚類)、榖類(例如水稻、 玉米)、植物(例如大豆)或其組合1等蛋白f之非限制性 實例包括如本文所述之乳蛋白分離物、乳蛋白濃縮物、路 蛋白分離物、乳清蛋白、酪蛋白鈉或酪蛋白鈣、全牛乳、 部分或完全脫脂乳、大豆蛋白分離物、大豆蛋白濃縮物 等。 碳水化合物 營養產品可進一步包含適合用於口服營養產品中且與該 等產品之基本要素及特徵相容的任何碳水化合物。以營養 液飲料之重量计,營養液飲料中之碳水化合物濃度例如可 在約5%至約40%之範圍内,包括約7%至約3〇%,包括約 10%至約25。/〇。在一特定實施例中,營養乳液中礙水化合 物之含量為以營養乳液之重量計約丨〇 2%。 158197.doc •29· 201212828 以固體營養產品之重量計,固體營養產品中之碳水化合 物濃度可在約10%至約90%之範圍内,包括約2〇%至約 8〇% ’進—步包括約40%至約60%。在一特定實施例中, 以噴霧乾燥營養粉之重量計’喷霧乾燥營養粉中碳水化合 物之含量為約58%。 適合用於本文所述之營養產品中之碳水化合物或其來源 的非限制性貫例可包括麥芽糊精、水解或改質殿粉或玉米 澱粉、葡萄糖聚合物、玉米糖漿、玉米糖漿固體、來源於 水稻之碳水化合物、來源於豌豆之碳水化合物、來源於馬 鈴薯之碳水化合物、木薯、蔗糖、葡萄糖、果糖、乳糖、 高果糖玉米糖漿、蜂蜜、糖醇(例如麥芽糖酵、赤藻糖 醇、山梨糖醇)、人造甜味劑(例如蔗糖素(sucral〇se)、乙 醢磺胺酸鉀、甜菊(stevia))及其組合。尤其所需之碳水化 合物為低右旋糖當量(DE)麥芽糊精。 脂肪 營養產品可進一步包含脂肪,最通常為乳化脂肪。以營 養乳液之重量計,營養液飲料中脂肪之含量可為〇%至約 30%,包括丨%至約30%,包括約1%至約2〇%,包括約⑼至 約15%,且亦包括約至約5%。在—特定實施例中,以 營養乳液之重量計,營養乳液包括約16%之量的脂肪。 熟習此項技術者應認識到,纟—些實施^列中,諸如在實 質上澄清液體飲料中,營養產品將實質上不含脂肪。 當用於固體營養產品中日夺,以目體營養產品之重量計, 脂肪之含量可為約1%至約35%,包括約1%至約鳩,包招 158197.doc •30· 201212828 約1%至約15% ,且亦包括約5 〇%至約1〇%。在—特定實施 例中,喷霧乾燥營養粉包括以喷霧乾燥營養粉之重量計約 7.5%之量的脂肪。 用於本文中之脂肪的適合來源包括適合用於口服營養產 品且與該等產品之基本要素及特徵相容的任何脂肪或脂肪The knife is about 10% to about 3 inches/〇. In the case of protein content _% in the _ A fog-dried nutrient powder, the sputum Ο is about 19%. The amount of 3 is suitable for the nutritional product by the weight of the spray-dried nutrient powder. Examples include non-limiting partial hydrolysis, partially hydrolyzed or non-hydrolyzed egg self-mass source of the heart or its source, which may be derived from any known source. Or otherwise suitable for ', < source, such as milk (such as casein, whey), animals (such as meat, fish), cockroaches (such as rice, corn), plants (such as soybeans) or a combination thereof 1 protein f Non-limiting examples include milk protein isolates, milk protein concentrates, road protein isolates, whey proteins, casein sodium or casein calcium, whole milk, partially or fully skimmed milk, soy protein separations as described herein. , soy protein concentrate, etc. The carbohydrate nutritional product may further comprise any carbohydrate suitable for use in oral nutritional products and compatible with the essential elements and characteristics of such products. The carbohydrate concentration in the nutrient solution beverage can be, for example, from about 5% to about 40% by weight of the nutrient solution beverage, including from about 7% to about 3%, including from about 10% to about 25. /〇. In a particular embodiment, the amount of water-blocking compound in the nutritional emulsion is about 2% by weight of the nutritional emulsion. 158197.doc •29· 201212828 The concentration of carbohydrates in the solid nutritional product may range from about 10% to about 90% by weight of the solid nutritional product, including from about 2% to about 8%. Includes from about 40% to about 60%. In a particular embodiment, the amount of carbohydrate in the spray dried nutritional powder is about 58% by weight of the spray dried nutritional powder. Non-limiting examples of carbohydrates or sources thereof suitable for use in the nutritional products described herein can include maltodextrin, hydrolyzed or modified temple flour or corn starch, glucose polymer, corn syrup, corn syrup solids, Carbohydrates derived from rice, carbohydrates derived from peas, carbohydrates derived from potatoes, cassava, sucrose, glucose, fructose, lactose, high fructose corn syrup, honey, sugar alcohols (eg maltase, erythritol, Sorbitol), artificial sweeteners (eg, sucralose, potassium sulfamate, stevia), and combinations thereof. A particularly desirable carbohydrate is a low dextrose equivalent (DE) maltodextrin. The fat nutritional product may further comprise fat, most commonly emulsified fat. The fat content of the nutrient solution beverage may range from 〇% to about 30%, including 丨% to about 30%, including from about 1% to about 2%, including from about (9) to about 15%, and It also includes about 5%. In a particular embodiment, the nutritional emulsion comprises about 16% by weight of the weight of the nutritional emulsion. Those skilled in the art will recognize that the nutritional products will be substantially free of fat, such as in a solid clarified liquid beverage. When used in a solid nutritional product, the fat content may be from about 1% to about 35%, including about 1% to about 5% by weight of the body nutrient product, including 158197.doc • 30· 201212828 1% to about 15%, and also includes about 5% to about 1%. In a particular embodiment, the spray dried nutritional powder comprises about 7.5% by weight of the weight of the spray dried nutritional powder. Suitable sources of fat for use herein include any fat or fat suitable for use in oral nutritional products and compatible with the essential elements and characteristics of such products.
適合用於本文所述之營養產品之脂肪或其來源的非限制 性實例包括椰子油、分餾椰子油、大豆油、玉米油、撖欖 油、紅花油、高油酸紅花油、MCT油(中鏈甘油三酯)、葵 花籽油、高油酸葵花籽油、棕櫊及棕櫚仁油、棕櫊油酸 (palm oleln)、菜籽油、海生動物油、棉籽油及其組合。 其他視情況選用之成分 包括嬰兒配方且具有本發明之豌豆蛋白水解物的營養產 品可進一步包含其他視情況選用之組分,其可改變產品之 物理、化學、美觀或加工特性或當用於目標群體中時充當 藥物或額外營養組分。許多該等視情況選用之成分為已知 的或另外適合用於醫學食物或其他營養產品或醫藥劑型中 且亦可用於本文之組合物中,其限制條件為該等視情況選 用之成分對於經口投藥為安全的且與所選產品形式中之基 本及其他成分相容。 該等視情況存在之成分的非限制性實例包括防腐劑、抗 氧化劑、乳化劑、緩衝劑、《募醣、半乳寡醣、人乳募醣 及其他益菌助生質(prebiotic)、醫藥活性劑、如本文所述 之其他營養素、著色劑、調味劑、增稠劑及穩定劑、乳化 158197.doc -31 - 201212828 劑、潤滑劑等。 可包括於本發明組合物中之適合HMO的實例包括乳糖-N-四糖、乳糖-N-新四糖、乳糖-N-岩藻五糖I、乳糖-N-岩 藻五糖II、乳糖-N-岩藻五糖III、乳糖-N-岩藻五糖V、乳 糖-N-六糖、對-乳糖-N-六糖、乳糖-N-新六糖、對-乳糖-N-新六糖、單岩藻糖基乳糖-N-六糖II、異構岩藻糖基化乳 糖-N-六糖(1)、單岩藻糖基乳糖-N-六糖、異構岩藻糖基化 乳糖-N-六糖(3)、異構岩藻糖基化乳糖-N-六糖(2)、二岩 藻糖基-對-乳糖-N-新六糖、二岩藻糖基-對-乳糖-N-六 糖、二岩藻糖基乳糖-N-六糖、乳糖-N-新八糖、對-乳糖-N-八糖、異-乳糖-N-八糖、乳糖-N-八糖、單岩藻糖基乳 糖-新八糖、單岩藻糖基乳糖-N-八糖、二岩藻糖基乳糖-N-八糖I、二岩藻糖基乳糖-N-八糖II、二岩藻糖基乳糖-N-新 八糖II、二岩藻糖基乳糖-N-新八糖I、乳糖-N-十糖、三岩 藻糖基乳糖-N-新八糖、三岩藻糖基乳糖-N-八糖、三岩藻 糖基-異-乳糖-N-八糖及其組合。此等HMO更全面地描述 於美國專利申請案第2009/0098240號中,該案以全文引用 的方式併入本文中。可包括於本發明組合物中之HMO的其 他適合實例包括乳糖-N-二岩藻糖-六糖II、唾液酸基α(2-3) 乳糖、唾液酸基α(2-6)乳糖、唾液酸基-乳糖-Ν-四糖a、唾 液酸基-乳糖-N-四糖b、唾液酸基-乳糖-N-四糖c、唾液酸 基-岩藻糖基-乳糖-N-四糖I、唾液酸基-岩藻糖基-乳糖-N-四糖II及二唾液酸基-乳糖-N-四糖。 組合物可進一步包含甜味劑,較佳包括至少一種糖醇, 158197.doc -32- 201212828 諸如麥芽糖醇、赤藻糖醇、山梨糖醇、木糖醇、甘露糖 醇、異麥芽糖及乳糖酵,且亦較佳包括至少一種人造或高 效力甜未劑,諸如乙醯續胺酸卸、阿斯巴甜糖 (aSpartame)、蔗糖素、糖精、甜菊及塔格糖(tagatose)。此 等甜味齊],尤其為糖醇與人造甜味劑之組合,尤其適用於 ' 調配具有所需有利概況之本發明液體飲料實施例。此等甜 未劑組合尤其有效掩蔽有時與液體飲料中添加植物蛋白相 〇 關之不良風味。以營養產品之重量計,營養產品中視情況 選用之糖醇濃度可在至少約001%之範圍内,包括01%至 ’·勺10 /〇且亦包括約1 °/0至約6%。以營養產品之重量計, 視N况選用之人造甜味劑濃度可在約〇 〗%之範圍内,包 括約0.05 /〇至約5% ’亦包括約〇丨%至約1 〇%。 机動劑或抗結塊劑可包括於營養產品(諸如本文所述之 s養粕)中以延緩粉末隨時間凝塊或結塊並製備容易自其 谷益流動之粉末實施例。已知或另外適合用於營養粉或產 ◎ αΠ形式中之任何已知流動劑或抗結塊劑適用於本文,其非 限制眭具例包括磷酸三鈣、矽酸鹽及其組合。營養產品中 桃動劑或抗結塊劑之濃度視產品形式、其他所選成分、所 需机動性質等而變化,但以營養產品之重量計,最通常在 約0.1%至約4%之範]u内,包括約〇5%至約2%。 I疋劑亦可包括於營養產品中。已知或另外適合用於營 養產°°中之任何穩定劑亦適用於本文,其一些非限制性實 例包括樹膠,諸如三仙膠。以營養產品之重量計,穩定劑 可佔約〇·1%至約5.0%,包括約0.5%至約3%,包括約〇.7% 158197.doc •33- 201212828 至約1.5%。 組合物可進一步包合各插甘认 乂匕3夕種其他維生素或相關營養素中之 任一者,其非限制性實例包括維生素A、維生素D、維生 素E、准生素K、硫胺素、核黃素、π比㈣、維生素〜' 類_素(例如Μ月蘿萄素、玉米黃素、葉黃素、番蘇红 素)、於酸、葉酸、泛酸、生物素、維生素c、膽驗、肌 醇、其鹽及衍生物,及其組合。 組合物可進一步包含多種其他另外礦物質中之任一者, 其非限制性實例包括妈、磷、鎂、鐵、辞、錳、銅、納、 鉀、鉬 '鉻、硒、氣化物及其組合。 製造方法 ,本發明之營養產品可藉由用於製備所選產品固體或液體 形式之任何已知或另外有效製造技術來製備。已知用於任 何特疋產物形式(諸如營養液或營養粉或營養棒)之許多該 等技術且其可容易由—般技術者應用於本文所述之營養組 合物。 因此,本發明之營養產品可藉由多種已知或另外有效調 配或製造方法中之任—者來製備。在—種適合之製造方法 幻女製備至少二種各別漿料,包括脂肪包蛋白質 in fat ’ PIF)漿料、碳水化合物_礦物質(ch〇 min) 水料及水包蛋白質(pr〇tein_in_water , piw)漿料。piF漿料 藉由加熱及混合所選油(例如菜籽油、玉米油等),隨後在 持續加熱及攪拌下添加乳化劑(例如印磷脂)、脂溶性維生 158197.doc •34· 201212828 素及一部分總蛋白質(例如乳蛋白濃縮物等)來形成。CHO-MIN漿料藉由在加熱攪拌下將以下物質添加至水中來形 成:礦物質(例如檸檬酸鉀、磷酸氫二鉀、檸檬酸鈉等)、 痕量及超痕量礦物質(TM/UTM預混物)、增稠劑或懸浮劑 (例如艾維素(avicel)、結蘭膠(gellan)、角叉菜膠)。所得 CHO-MIN漿料在持續加熱及攪拌下保持10分鐘,隨後添加 其他礦物質(例如氯化鉀、碳酸鎂、碘化鉀等)及/或碳水化 合物(例如果募醣、蔗糖、玉米糖漿等)。接著藉由在加熱 及攪拌下混合其餘蛋白質(若存在)來形成PIW漿料。 接著將所得漿料在加熱攪拌下摻合在一起且將pH值調整 至6.6-7.0,隨後對組合物進行高溫短時(HTST)處理,在此 期間將組合物熱處理、乳化及均質化,接著使其冷卻。添 加水溶性維生素及抗壞血酸,必要時將pH值調整至所需範 圍,添加調味劑,且添加水以達成所需總固體含量。接著 無菌包裝組合物以形成無菌包裝營養乳液。接著可進一步 稀釋、熱處理及包裝此乳液以形成即食或濃縮液體,或可 將其熱處理且隨後加工及包裝成可復原粉末,例如噴霧乾 燥、乾式混合、聚結。 當需要實質上澄清液體飲料時,修改上述製造方法以使 得僅摻合CHO-MIN漿料與PIW漿料以形成營養液飲料。 諸如營養棒之其他產品形式可例如使用如棒狀物製造技 術中已知及通常所述之冷擠壓技術來製造。為製備該等產 品,通常將所有粉末狀組分乾式摻合在一起,其通常包括 158197.doc -35- 201212828 任何蛋白質、維生素預混物、特定碳水化合物等。接著將 月曰溶性組分摻合在—起並與任何預混物粉末混合。最後, 將任何液體組分混入組合物中,形成塑膠樣組合物或團狀 物。接著可藉由冷成型或擠壓使所得塑膠塊成形,而不發 生物理或化學變化,其中在相對低的壓力下迫使該塑膠塊 通過賦予所需形狀之模具。接著在適當位置切斷所得流出 物,彳寸到所需重量之產品。必要時,接著塗佈固體產物, 以增強可口性,並包裝用於分配。 其他適合用於製備營養產品之方法例如描述於美國專利 第 6,365,218 號(B0rschel 等人)、美國專利 6 589 576 (B〇rSChel等人)、美國專利第6,306,908號(Carlson等人)、 美國專利申δ月案20030118703 A1 (Nguyen等人)中,該等描 述在其與此—致之程度上以引用的方式併入本文中。 使用方法 包括豌五蛋白水解物作為蛋白質之唯一來源的營養產品 適用作用於對牛乳蛋白、大豆蛋白不耐及/或過敏及/或具 有其他一般食物過敏之早產兒、嬰兒、幼兒、兒童、成人 及老年人的低過敏性營養來源及/或半要素營養來源。另 外,營養產品可尤其適合用於具有吸收障礙、消化不良及 其他胃腸病狀之個體。 在一實施例中,營養產品可適用於提供使用水解蛋白質 且具有改良感官性質之營養來源’諸如運動飲料中。 營養產品可按需要經口投與以提供所需營養含量,最通 158197.doc • 36 · 201212828 三份、.四份、五份、六份、七份、 份量大小可變化很大且可在約1 mI 包括約5 ml至約250 m卜包括約1〇 〇 以下實例說明本發明之腕豆蛋白水解物及/或營養產品 的特定實施例及/或特徵。該等實例僅出於說明之目的而 給出且不應理解為限制本發明,因為在不障離本發明之精 神及範疇的情況下其許多蠻化可左 t夕變化了犯存在。除非另外規定, 否則所有例示量均為以組合物之總重 一 ϋ L室量计的重量百分比。 例示組合物為根據營養行聿中孰 仃杲Τ熟知用於製備營養乳液、 其他營養液飲料、營養粉及/或營養 呂贅棒之製造方法所製備 之營養產品。 實例1 〇 常以每日一份、兩份 八份或更多份之形式 至约500 ml之範圍内 ml至約 240 ml。 在此實例中,評估根據如本文 、*,r、 4〈万法所製備的經過 慮⑺及未經過滤(N)婉豆蛋白水解物之iQ個樣品且^敕 婉立蛋白分離物剛樣品比較各種物理特性 '經過: 表示樣品進行上述第二過濾步驟。 "; 所有樣品均進行上述第 一過濾步驟。對照樣品(完整)不進耔紅y _ 仃任何水解程序。特定 吕之’使用標準分析技術評估樣品之 .. 解度、分子量分 佈、異黃_含量及植酸鹽含量。評估社 _ 、、’Q果展示於下表中。 158197.doc -37- 201212828 樣品 完整 AN AF BN BF CN CF DN DF EN EF 屬性 氮% 12.90 13.30 13.60 13.20 14.00 12.90 13.60 13.10 14.00 12.90 13.90 蛋白質% 80.63 83.13 85 82.5 87.5 80.63 85 81.88 87.5 80.63 86.88 氮%\4~近似值6.25 來自AA B+F% 之 蛋白質 82.05 81.34 88.83 81.12 88.64 80.20 83.03 82.70 91.27 83.65 94.36 胺基N 1.12 1.68 2.05 2.2 2.39 2.88 2.98 2.95 3.31 3.41 3.97 DH(% AN/TN) 8.68 12.63 15.07 16.67 17.07 22.17 21.91 22.75 23.64 26.43 28.56 經調整 DH 0.00 3.95 6.39 7.99 8.39 13.49 13.23 14.07 14.96 17.75 19.88 在每毫 升5 mg 蛋白質 下100 ml之毫 克數 620 601 588 606 571 620 588 610 571 620 576 在2%蛋 白質下 Gm Par =500 ml 12.4 12.02 13.75 12.12 11.42 12.4 11.76 12.2 11.42 12.4 11.52 根據HPLC之MW範園 >3KD 97.00% 37.50% 27.20% 10.40% 4.80% 6.70% 1.70% 6.90% 2.60% 5.80% 1.60% >5KD 93.30% 26.30% 16.50% 4.10% 1.00% 2.70% 0.40% 2.50% 0.60% 2.00% 0.40% 3-5 KD 3.70% 11.20% 10.70% 6.30% 3.80% 4.00% 1.30% 4.40% 2.00% 3.80% 1.20% 1-3 KD 1.00% 28.80% 34.50% 31.30% 33.10% 18.10% 17.80% 22.90% 24.60% 18.40% 19.20% 0.5-1 KD 0.40% 20.30% 23.60% 30.40% 33.20% 29.30% 31.80% 29.40% 31.60% 28.10% 30.30% <0.5 KD 1.60% 13.40% 14.70% 27.90% 28.90% 45.90% 48.70% 40.80% 41.20% 47.70% 48.90% 植物雌激素含量 大豆異 黃 酮 (mg/kg) <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 染料木 黃 蜩 (mg/kg) <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 總 磷 (g/kg) 8.3 8.55 6.41 8.3 4.58 8.27 5.73 8.27 5.05 8.21 4.89 植 酸 (g/kg) 20.6 21.2 15.9 20.6 11.4 20.6 14.2 20.6 12.5 20.4 12.2 -38- 158197.doc 201212828 實例2 在此實例中,評估實例1中所分析之豌豆蛋白水解物的 低過敏性可能性且使用兔子超免疫與實例1之完整豌豆蛋 白分離物(ISO)樣品比較’其中如下文所述經35天之時期 給與兔子多次免疫。接著藉由ELIS A(酶聯免疫吸附檢定) 測試豌豆蛋白水解物之免疫原性,其量測兔子對完整婉豆 蛋白/婉豆蛋白水解物免疫原之抗原特異性IgG反應。 〇 在第一次免疫之前,使此實例中所用之兔子進行不含牛 乳、大豆及豌豆蛋白之膳食14天。第〇天,藉由來自兔子 之血液樣品評估每一兔子之基線抗體狀況。獲得基線後, 用以完全弗氏佐劑(Complete Freund's Adjuvant,CFA)乳 化之4.5 ml抗原中所含的5.0 mg婉豆蛋白當量胺基酸含量 對每一兔子免疫接種。如下傳遞免疫:每—部位2〇χ〇1 ml/去毛側腹,皮内;及0.25 ml/後腿胭淋巴結區。第21 天’第二次免疫如下:每一部位〇5〇 mlx2處部位/後腿, 〇 肌肉内;及每一部位0.25 mlx4處部位,肩胛下。對於每一 次免疫,投與5 mg恆定劑量之抗原胺基酸當量。在研究第 35天將兔子放血。 使用直接ELISA定量超免疫後每一兔子之抗體反應。使 •用阻斷滴定達成檢定最佳化以測定抗原塗佈濃度及結合物 稀釋度。檢定最佳化後,定量第〇天及第35天血清樣品中 之豌豆蛋白特異性兔子抗體。另外,藉由將經校正之第h 天抗體效價除以每一抗血清第〇天之經校正基線(第〇天)效 價來測定免疫反應指數(IRI單位)^免疫組含有5_1〇隻動物 158197.doc -39- 201212828 且對各動物之結果取平均值。抗原特異性IRI結果之比較 預示低過敏性臨床效能。結果提供於下表中: 超免疫後之免疫反應指數(經校正之第35天效價/經校正之第0天效價) 完整 AN AF BN BF CN CF DN DF EN EF IRI 單位 7155 9002 7558 330.8 167.8 167.2 10.73 921.4 932.8 1027 144.5 Log IRI 單位 3.8546 3.9543 3.8784 2.5196 2.2245 2.2232 1.0307 2.9644 2.9698 3.0115 2.16 如該表中所示,豌豆蛋白水解物顯示寬泛範圍之抗體反 應。低反應性可表明豌豆蛋白水解物仍具有由兔子識別為 抗原性而非免疫原性之抗原決定基。另外,在超免疫後, 與其他樣品相比,豌豆蛋白水解物BN、BF、CN及CF顯示 顯著降低之免疫原性。豌豆蛋白水解物樣品降低之免疫原 性表明保留之豌豆抗原不具有刺激免疫反應之能力。 實例3 在此實例中,評估實例1中所分析之豌豆蛋白水解物的 低過敏性可能性且使用藉由抑制ELISA量測之抗原含量與 實例1之完整腕豆蛋白分離物(ISO)樣品比較。在此檢定 中,將完整豌豆蛋白塗佈於ELISA培養板上。各別地準備 含有標準濃度之樣品或豌豆蛋白溶液加上抗豌豆蛋白抗體 的培育管。培育期後,將樣品(或標準物)加上抗體溶液之 等分試樣添加至經塗佈之培養板中。培育及洗滌後,添加 抗兔子IgG酶結合抗體。培育培養板,洗滌,且添加酶受 質。進行顯色反應,其與基於標準曲線定量之樣品中豌豆 抗原之濃度成反比。10個樣品之此測試結果展示於下表 158197.doc -40- 201212828 中: 超免疫後之免疫反應指數(經校正之第35天效償/經校正之第0天效價) 穿琴 7\j at. AN AF BN BF CN CF DN DF EN EF 抗原 (pg/g 蛋 白質) 1,000,000 493009 431210 55413 36007 15013 6417 18504 14900 14215 8644 實例4 在此實例中,進行感官評估持續兩階段篩選期以表徵蒸 ◎ 顧於水與 Alimentum® 基質(Abbott Laboratories, Columbus, Ohio)中之實例1之豌豆蛋白水解物樣品的味道。該 Alimentum®基質含有礙水化合物、脂肪、維生素及礦物 質以模擬低過敏性配方之生物化學。其用於評估對水解物 之相互感官影響及最終配方對蒸餾處理後產品基質内彼此 之影響。將樣品與Alimentum®中所用之當前水解物(酷·蛋 白水解物)及實例1之完整豌豆蛋白分離物(ISO)進行比較。 特定言之,已知水解蛋白質一般為苦味、酸味、贼味及/ 〇 或豆味。因此,難以在不強烈損害產品味道之情況下將此 功能成分併入營養調配物中。 分析樣品之各種味道強度,包括例如:苦味、鹹味、酸 味、鮮味、濕羊毛味、酸的酸味、青草味/大豆味、澱粉 味、木頭味/木屑味、生青味/豆味/乾豆味、乾燥、粉末 狀、油性、硫化物味/卷心菜味、燒奶絡味及粗砂質。特 定言之,一旦樣品製備後,即令四個受過訓練之描述風味 官能檢查員品嘗每一樣品。品嘗後,每一官能檢查員使用 158197.doc -41 - 201212828 下表中所示之評分系統評估樣品之風味屬性且接著對結果 取平均值。 分數 描述 */2 極輕微 1 輕微 1 */2 輕微至中等 2 中等 2½ 中等至強烈 3 強烈 水中之水解物(2%溶液) 基礎味道 及芳香味 AF BF CF DF EF 當前 Alimentum® 水解物 苦味 1 1 */2 1 1 1 1 械味 1 1 】/2 */2 1 酸味 */2 '/2 y2 */2 1 1 鮮味 1 1 1 */2 1 y2 2 2½ 濕羊毛味 1 */2 1 ^2 1 */2 1 */2 2 2½ 酸的酸味 1 外觀: 半透明 淺黃色 半透明 淺黃色 半透明 淺黃色 半透明 淺黃色 半透明 淺黃色 半透明金黃色 水中之未經過濾水解物(2%溶液) 基礎味道及芳香味 AN BN CN DN ΕΝ ISO 苦味 1 1 1 !/2 1 1 */2 械味 1 */2 酸味 1 1 1 1 1 1 158197.doc •42· 201212828Non-limiting examples of fats or sources thereof suitable for use in the nutritional products described herein include coconut oil, fractionated coconut oil, soybean oil, corn oil, eucalyptus oil, safflower oil, high oleic safflower oil, MCT oil (medium Chain triglyceride), sunflower oil, high oleic sunflower oil, palm and palm kernel oil, palm oleln, rapeseed oil, marine animal oil, cottonseed oil and combinations thereof. Other optional ingredients including infant formulas and nutritional products having the pea protein hydrolysate of the present invention may further comprise other optional components which may alter the physical, chemical, aesthetic or processing characteristics of the product or when used in the target Act as a drug or an additional nutrient component in the population. Many of these optional ingredients are known or otherwise suitable for use in medical foods or other nutritional products or pharmaceutical dosage forms and may also be used in the compositions herein, with the proviso that the ingredients selected as such Oral administration is safe and compatible with the essential and other ingredients in the selected product form. Non-limiting examples of such optional ingredients include preservatives, antioxidants, emulsifiers, buffers, "sugar, galactooligosaccharides, human milk, and other probiotics, pharmaceutically active Agents, other nutrients, colorants, flavoring agents, thickening agents and stabilizers as described herein, emulsification 158197.doc -31 - 201212828 agents, lubricants, and the like. Examples of suitable HMOs which may be included in the compositions of the present invention include lactose-N-tetraose, lactose-N-neotetraose, lactose-N-fucos pentasaccharide I, lactose-N-fucos pentasaccharide II, lactose -N-fucoid pentasaccharide III, lactose-N-fucoid pentasaccharide V, lactose-N-hexasaccharide, p-lactose-N-hexaose, lactose-N-hexaose, p-lactose-N-new Hexaose, monofucosyl lactose-N-hexasaccharide II, isomerized fucosylated lactose-N-hexasaccharide (1), monofucosyl lactose-N-hexose, isomerized fucose Lactose-N-hexasaccharide (3), isomerized fucosylated lactose-N-hexaose (2), difucosyl-p-lactose-N-hexaose, difucosyl -p-lactose-N-hexaose, difucosyllactose-N-hexaose, lactose-N-new octasaccharide, p-lactose-N-octasaccharide, iso-lactose-N-octasaccharide, lactose- N-octasaccharide, monofucosyl lactose-new octasaccharide, monofucosyl lactose-N-octasaccharide, difucosyllactose-N-octasaccharide I, difucosyllactose-N- Octaose II, difucosyllactose-N-new octasaccharide II, difucosyllactose-N-new octasaccharide I, lactose-N-decasaccharide, trifucosyl lactose-N-Xinba Sugar, trifucosyl lactose-N-octasaccharide, trichophyton Glycosyl-iso-lactose-N-octasaccharide and combinations thereof. Such HMOs are more fully described in U.S. Patent Application Serial No. 2009/0098240, which is incorporated herein in its entirety by reference. Other suitable examples of HMOs which may be included in the compositions of the present invention include lactose-N-digofose-hexasaccharide II, sialic acid alpha (2-3) lactose, sialic acid alpha (2-6) lactose, Sialyl-lactose-indole-tetraose a, sialyl-lactose-N-tetraose b, sialyl-lactose-N-tetraose c, sialic acid-fucosyl-lactose-N-four Sugar I, sialyl-fucosyl-lactose-N-tetraose II and disialyl-lactose-N-tetraose. The composition may further comprise a sweetener, preferably comprising at least one sugar alcohol, 158197.doc -32 - 201212828 such as maltitol, erythritol, sorbitol, xylitol, mannitol, isomaltose and lactose And preferably, at least one artificial or high-potency sweetener, such as acetaminophen, aSpartame, sucralose, saccharin, stevia, and tagatose. Such sweetnesses, especially combinations of sugar alcohols and artificial sweeteners, are particularly suitable for use in formulating liquid beverage embodiments of the invention having the desired advantageous profile. These sweetener combinations are particularly effective in masking undesirable flavors that are sometimes associated with the addition of vegetable proteins to liquid beverages. The concentration of sugar alcohol selected in the nutritional product may be in the range of at least about 001% by weight of the nutritional product, including 01% to ’·spoon 10 〇 and also including about 1 °/0 to about 6%. The artificial sweetener concentration, depending on the weight of the nutritional product, may range from about 〇%, including from about 0.05/〇 to about 5% ‘, including from about 〇丨% to about 〇%. The motorized or anti-caking agent can be included in a nutritional product, such as the sputum described herein, to delay the agglomeration or agglomeration of the powder over time and to prepare a powder embodiment that readily flows from its base. Any known flow or anti-caking agent known or otherwise suitable for use in nutritional powders or in the form of ΠαΠ is suitable for use herein, non-limiting examples of which include tricalcium phosphate, citrate, and combinations thereof. The concentration of the peach or anti-caking agent in the nutritional product varies depending on the product form, other selected ingredients, the required maneuverability, etc., but is usually from about 0.1% to about 4% by weight of the nutritional product. ]u, including about 5% to about 2%. I can also be included in nutritional products. Any stabilizer known or otherwise suitable for use in a nutritional formulation is also suitable for use herein, some non-limiting examples of which include gums such as triterpene. The stabilizer may comprise from about 1% to about 5.0% by weight of the nutritional product, including from about 0.5% to about 3%, including about 7%.7% 158197.doc • 33-201212828 to about 1.5%. The composition may further comprise any of the other vitamins or related nutrients of each of the inserted sweets, non-limiting examples of which include vitamin A, vitamin D, vitamin E, vitamin K, thiamine, Riboflavin, π ratio (IV), vitamin ~ 'class _ (eg Μ月萝素, zeaxanthin, lutein, sulphonin), acid, folic acid, pantothenic acid, biotin, vitamin c, biliary test, Inositol, its salts and derivatives, and combinations thereof. The composition may further comprise any of a variety of other additional minerals, non-limiting examples of which include mom, phosphorus, magnesium, iron, rhenium, manganese, copper, sodium, potassium, molybdenum 'chromium, selenium, vapors and their combination. Method of Manufacture, the nutritional product of the present invention can be prepared by any known or otherwise effective manufacturing technique for preparing a solid or liquid form of the selected product. Many of these techniques are known for use in any particular product form, such as nutrient solutions or nutritional powders or nutritional bars, and can be readily applied by the general practitioner to the nutritional compositions described herein. Thus, the nutritional product of the present invention can be prepared by any of a variety of known or otherwise effective methods of formulation or manufacture. In a suitable manufacturing method, the phantom prepares at least two separate pastes, including fat-packed protein in fat 'PIF) slurry, carbohydrate-mineral (ch〇min) water and water-packed protein (pr〇tein_in_water, Piw) slurry. piF slurry by heating and mixing selected oils (such as rapeseed oil, corn oil, etc.), followed by continuous heating and stirring to add an emulsifier (such as imprinted phospholipids), fat-soluble vitamins 158197.doc • 34· 201212828 And a part of total protein (such as milk protein concentrate, etc.) is formed. CHO-MIN slurry is formed by adding the following substances to water under heating and stirring: minerals (such as potassium citrate, dipotassium hydrogen phosphate, sodium citrate, etc.), traces and ultra-trace minerals (TM/ UTM premix), thickener or suspending agent (eg avicel, gellan, carrageenan). The obtained CHO-MIN slurry is kept under heating and stirring for 10 minutes, and then other minerals (such as potassium chloride, magnesium carbonate, potassium iodide, etc.) and/or carbohydrates (for example, sugar, sucrose, corn syrup, etc.) are added. . The PIW slurry is then formed by mixing the remaining protein (if present) with heating and stirring. The resulting slurry is then blended together under heat and agitation and the pH is adjusted to 6.6-7.0, after which the composition is subjected to a high temperature short time (HTST) treatment during which the composition is heat treated, emulsified and homogenized, followed by Let it cool. Water-soluble vitamins and ascorbic acid are added, pH is adjusted to the desired range if necessary, flavoring is added, and water is added to achieve the desired total solids content. The composition is then aseptically packaged to form a sterile packaged nutritional emulsion. The emulsion can then be further diluted, heat treated and packaged to form a ready-to-eat or concentrated liquid, or it can be heat treated and subsequently processed and packaged into a reconstitutable powder, such as spray drying, dry mixing, coalescence. When it is desired to substantially clarify the liquid beverage, the above manufacturing method is modified so that only the CHO-MIN slurry and the PIW slurry are blended to form a nutrient solution beverage. Other product forms such as nutritional bars can be made, for example, using cold extrusion techniques known in the art of rod manufacturing and generally described. To prepare such products, all of the powdered components are typically dry blended together, which typically includes 158197.doc -35 - 201212828 any protein, vitamin premix, specific carbohydrates, and the like. The lunar lysate component is then blended in and mixed with any premix powder. Finally, any liquid component is mixed into the composition to form a plastic-like composition or agglomerate. The resulting plastic block can then be formed by cold forming or extrusion without biological or chemical changes, wherein the plastic block is forced through a mold that imparts the desired shape at a relatively low pressure. The resulting effluent is then cut at the appropriate location and the product is weighed to the desired weight. If necessary, the solid product is then coated to enhance palatability and packaged for dispensing. Other suitable methods for the preparation of nutritional products are described, for example, in U.S. Patent No. 6,365,218 (B0schel et al.), U.S. Patent No. 6,589,576 (B.r., et al.), U.S. Patent No. 6,306,908 (Carlson et al.), U.S. Patent Application In the case of δ月月20030118703 A1 (Nguyen et al.), the descriptions are hereby incorporated by reference. The method of use includes pea protein hydrolysate as the sole source of protein. The nutritional product is suitable for premature infants, infants, young children, children, adults who are intolerant to and/or allergic to milk protein, soy protein, and/or other general food allergies. And hypoallergenic nutrient sources and/or semi-factorial sources of nutrition for the elderly. In addition, nutritional products are particularly suitable for use in individuals with malabsorption, dyspepsia and other gastrointestinal conditions. In one embodiment, the nutritional product may be adapted to provide a source of nutrients such as sports drinks that use hydrolyzed proteins and have improved organoleptic properties. Nutritional products can be administered orally as needed to provide the required nutrient content. The most common 158197.doc • 36 · 201212828 three, four, five, six, seven, the size can vary greatly and can be A specific embodiment and/or feature of the caramel protein hydrolysate and/or nutritional product of the present invention is illustrated by an example of about 1 mI, including from about 5 ml to about 250 m, including about 1 Torr. The examples are given for illustrative purposes only and are not to be construed as limiting the invention, as many of the singularities of the present invention can be changed without departing from the spirit and scope of the invention. Unless otherwise stated, all exemplified amounts are by weight based on the total weight of the composition. The exemplified composition is a nutritional product prepared according to the manufacturing method for preparing a nutritional emulsion, other nutrient liquid beverage, nutritional powder, and/or nutrition 赘 赘 according to 营养 营养. Example 1 〇 Often one part per day, two servings of eight or more servings to a range of about 500 ml to about 240 ml. In this example, iQ samples of the treated (7) and unfiltered (N) cowpea protein hydrolysates prepared according to the methods herein, *, r, 4, and 10,000 are evaluated. Comparison of various physical properties 'passing: indicates that the sample is subjected to the second filtering step described above. "; All samples were subjected to the first filtration step described above. The control sample (complete) did not enter the erythro y _ 仃 any hydrolysis procedure. Specific Lvzhi's use standard analytical techniques to evaluate samples. Resolution, molecular weight distribution, iso-yarn content, and phytate content. The evaluation agency _ , , 'Q results are shown in the table below. 158197.doc -37- 201212828 Sample complete AN AF BN BF CN CF DN DF EN EF Property nitrogen % 12.90 13.30 13.60 13.20 14.00 12.90 13.60 13.10 14.00 12.90 13.90 Protein % 80.63 83.13 85 82.5 87.5 80.63 85 81.88 87.5 80.63 86.88 Nitrogen%\4 ~ Approximate value 6.25 Protein from AA B+F% 82.05 81.34 88.83 81.12 88.64 80.20 83.03 82.70 91.27 83.65 94.36 Amine N 1.12 1.68 2.05 2.2 2.39 2.88 2.98 2.95 3.31 3.41 3.97 DH(% AN/TN) 8.68 12.63 15.07 16.67 17.07 22.17 21.91 22.75 23.64 26.43 28.56 Adjusted DH 0.00 3.95 6.39 7.99 8.39 13.49 13.23 14.07 14.96 17.75 19.88 100 ml of milligrams per ml of 5 mg protein 620 601 588 606 571 620 588 610 571 620 620 576 Gm Par = 2 at 2% protein Ml 12.4 12.02 13.75 12.12 11.42 12.4 11.76 12.2 11.42 12.4 11.52 MW Fanyuan according to HPLC>3KD 97.00% 37.50% 27.20% 10.40% 4.80% 6.70% 1.70% 6.90% 2.60% 5.80% 1.60% >5KD 93.30% 26.30% 16.50% 4.10% 1.00% 2.70% 0.40% 2.50% 0.60% 2.00% 0.40% 3-5 KD 3.70% 11.20% 10.70% 6. 30% 3.80% 4.00% 1.30% 4.40% 2.00% 3.80% 1.20% 1-3 KD 1.00% 28.80% 34.50% 31.30% 33.10% 18.10% 17.80% 22.90% 24.60% 18.40% 19.20% 0.5-1 KD 0.40% 20.30% 23.60% 30.40% 33.20% 29.30% 31.80% 29.40% 31.60% 28.10% 30.30% <0.5 KD 1.60% 13.40% 14.70% 27.90% 28.90% 45.90% 48.70% 40.80% 41.20% 47.70% 48.90% Phytoestrogens Content Soy Flavonoid (mg/kg) <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 <2>2 dye wood xanthine (mg/kg) < 2 <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 <2 Total phosphorus (g/kg) 8.3 8.55 6.41 8.3 4.58 8.27 5.73 8.27 5.05 8.21 4.89 Acid (g/kg) 20.6 21.2 15.9 20.6 11.4 20.6 14.2 20.6 12.5 20.4 12.2 -38- 158197.doc 201212828 Example 2 In this example, the hypoallergenic possibility of the pea protein hydrolysate analyzed in Example 1 was evaluated and used. Rabbit hyperimmunization was compared to the complete pea protein isolate (ISO) sample of Example 1 'where multiple immunizations were given to rabbits over a 35 day period as described below. The immunogenicity of the pea protein hydrolysate was then tested by ELIS A (enzyme-linked immunosorbent assay), which measures the antigen-specific IgG response of the rabbit to the intact cowpea/homoprotein hydrolysate immunogen.兔子 The rabbits used in this example were subjected to a diet containing no milk, soy and pea protein for 14 days prior to the first immunization. On the third day, the baseline antibody status of each rabbit was evaluated by blood samples from rabbits. After the baseline was obtained, each rabbit was immunized with a 5.0 mg cowpea protein equivalent amino acid content contained in 4.5 ml of antigen emulsified in Complete Freund's Adjuvant (CFA). Immunization was delivered as follows: 2 〇χ〇 1 ml per site - depilated flank, intradermal; and 0.25 ml / hind leg axillary lymph node area. On the 21st day, the second immunization was as follows: each part 〇5〇 mlx2 part/hind leg, 〇 muscle; and 0.25 ml×4 parts of each part, under the shoulder. For each immunization, a constant dose of 5 mg of the amino acid equivalent was administered. The rabbits were bled on the 35th day of the study. The antibody response of each rabbit after hyperimmunization was quantified using a direct ELISA. • Optimize the assay by blocking titration to determine antigen coating concentration and conjugate dilution. After the assay was optimized, the pea protein-specific rabbit antibodies in the serum samples on Days 1-3 and Day 35 were quantified. In addition, the immune response index (IRI unit) was determined by dividing the corrected h-day antibody titer by the corrected baseline (days) titer of each antiserum on the third day. The immunized group contained 5_1〇. Animals 158197.doc -39- 201212828 and the results for each animal were averaged. Comparison of antigen-specific IRI results predicts hypoallergenic clinical efficacy. The results are provided in the table below: Immunoreactivity index after hyperimmunization (corrected 35th day titer / corrected 0th day titer) Complete AN AF BN BF CN CF DN DF EN EF IRI Unit 7155 9002 7558 330.8 167.8 167.2 10.73 921.4 932.8 1027 144.5 Log IRI Unit 3.8546 3.9543 3.8784 2.5196 2.2245 2.2232 1.0307 2.9644 2.9698 3.0115 2.16 As shown in the table, pea protein hydrolysate shows a wide range of antibody responses. Low reactivity may indicate that the pea protein hydrolysate still has an epitope that is recognized by the rabbit as antigenic rather than immunogenic. In addition, after hyperimmunization, pea protein hydrolysates BN, BF, CN and CF showed significantly reduced immunogenicity compared to other samples. The reduced immunogenicity of the pea protein hydrolysate sample indicates that the retained pea antigen does not have the ability to stimulate an immune response. Example 3 In this example, the hypoallergenic potential of the pea protein hydrolysate analyzed in Example 1 was evaluated and the antigen content measured by inhibition ELISA was compared to the intact carnid protein isolate (ISO) sample of Example 1. . In this assay, intact pea protein was plated onto an ELISA plate. Prepare tubes containing standard concentrations of samples or pea protein solutions plus anti-pea protein antibodies. After the incubation period, an aliquot of the sample (or standard) plus antibody solution is added to the coated culture plate. After incubation and washing, an anti-rabbit IgG enzyme-binding antibody was added. The plate was incubated, washed, and the enzyme was added. A color reaction is performed which is inversely proportional to the concentration of the pea antigen in the sample quantified based on the standard curve. The results of this test for 10 samples are shown in the following table 158197.doc -40- 201212828: Immune response index after hyperimmunization (corrected 35th day of reimbursement / corrected 0th day titer). j at. AN AF BN BF CN CF DN DF EN EF antigen (pg/g protein) 1,000,000 493009 431210 55413 36007 15013 6417 18504 14900 14215 8644 Example 4 In this example, sensory evaluation was performed for a two-stage screening period to characterize steaming ◎ Consider the taste of the pea protein hydrolysate sample of Example 1 in water and Alimentum® matrix (Abbott Laboratories, Columbus, Ohio). The Alimentum® matrix contains biochemicals that impede water compounds, fats, vitamins and minerals to mimic hypoallergenic formulations. It is used to assess the mutual sensory effects on the hydrolysate and the effect of the final formulation on each other within the matrix of the product after distillation. The samples were compared to the current hydrolysate (Cool Protein Hydrolysate) used in Alimentum® and the intact pea protein isolate (ISO) of Example 1. In particular, hydrolyzed proteins are generally known to be bitter, sour, thief, and/or odor. Therefore, it is difficult to incorporate this functional ingredient into the nutritional formulation without damaging the taste of the product. Analyze the various flavor strengths of the sample, including, for example, bitter, salty, sour, umami, wet wool, sour acid, grassy/soybean, starchy, woody/woody, green, bean/dry Bean flavor, dry, powdery, oily, sulphide/cabbage, roasted milk and coarse sand. Specifically, once the sample is prepared, each of the four trained flavor functional inspectors tastes each sample. After tasting, each functional inspector evaluates the flavor properties of the samples using the scoring system shown in the table below and then averages the results. Fractional description*/2 Very slight 1 Minor 1 */2 Mild to medium 2 Medium 21⁄2 Medium to strong 3 Hydrolysate in strong water (2% solution) Base taste and aroma AF BF CF DF EF Current Alimentum® Hydrolysate Bitter 1 1 */2 1 1 1 1 味味 1 1 】/2 */2 1 sour */2 '/2 y2 */2 1 1 umami 1 1 1 */2 1 y2 2 21⁄2 wet wool 1 */ 2 1 ^2 1 */2 1 */2 2 21⁄2 Acidic acidity 1 Appearance: Translucent light yellow translucent light yellow translucent light yellow translucent light yellow translucent light yellow translucent gold yellow water unfiltered hydrolysis (2% solution) Basic taste and aromatic taste AN BN CN DN ΕΝ ISO Bitter taste 1 1 1 !/2 1 1 */2 Mechanical taste 1 */2 Sour taste 1 1 1 1 1 1 158197.doc •42· 201212828
鮮味 1 1 1 1 1 */2 酸的酸味 1 青草味/大豆味 2 2 1 1 /2 1 */2 澱粉味 1 1 1 1 1 1 木頭味/木屑味 2 1 1 生青味/豆味/乾豆味 1 */2 乾燥 2 2 2 2 2 2 粉末狀口感 1 'Λ 1 */2 1 */2 1 »/2 1 */2 1 */2 外觀: 混濁黃 褐色 混濁黃 褐色 混濁黃 褐色 混濁黃 褐色 混濁黃 褐色 不透明淺 黃褐色Tasty 1 1 1 1 1 */2 Acidic sourness 1 Green grassy/soybean flavor 2 2 1 1 /2 1 */2 Starch flavor 1 1 1 1 1 1 Woody flavor / woody flavor 2 1 1 Green flavor / bean Taste/dry bean flavor 1 */2 Dry 2 2 2 2 2 2 Powdery mouthfeel 1 'Λ 1 */2 1 */2 1 »/2 1 */2 1 */2 Appearance: turbid yellow brown turbid yellow brown Turbid yellow brown turbid yellow brown turbid yellow brown opaque light yellow brown
Alimentum基質中之經過遽水解物 基礎味道 及芳香味 AF BF CF DF EF 當前 Alimentum® 水解物 苦味 */2 % */2 */2 1 1 械味 '/2 »/2 */2 */2 1 酸味 1 1 1 1 1 1 甜味 1 '/2 1 % 1 1 '/2 1 */2 1 */2 鮮味 2,火雞 1 ^2 1 */2 2,火雞 2,火雞 1 /2 酸的酸味 1 1 1 1 1 1 '/2 乾燥 1 1 1 1 1 1 油性口感 1 1 1 1 1 1 硫化物味/ 卷心菜味 2 燒奶酪味 1 »/2 濕羊毛味 】/2 外觀: 不透明淺灰 色及黃色 不透明 淺灰色 不透明 淺灰色 不透明 淺灰色 不透明 淺灰色 不透明淺灰色 158197.doc • 43- 201212828The base taste and aroma of the hydrazine hydrolysate in the Alimentum matrix AF BF CF DF EF The current Alimentum® hydrolysate bitterness */2 % */2 */2 1 1 mechanical taste '/2 »/2 */2 */2 1 sour 1 1 1 1 1 1 sweet 1 '/2 1 % 1 1 '/2 1 */2 1 */2 umami 2, turkey 1 ^ 2 1 */2 2, turkey 2, turkey 1 /2 Acidic acidity 1 1 1 1 1 1 '/2 Drying 1 1 1 1 1 1 Oily mouthfeel 1 1 1 1 1 1 Sulfate flavor / Cabbage flavor 2 Cheese flavor 1 »/2 Wet wool flavor /2 Appearance: opaque light gray and yellow opaque light gray opaque light gray opaque light gray opaque light gray opaque light gray 158197.doc • 43- 201212828
Alimentum基質中之未經過遽水解物 基礎味道及芳香味 AN* BN* CN* DN* EN* ISO* 苦味 1 1 */2 1 y2 1 % 1 */2 */2 械味 */2 y2 '/2 '/2 »/2 '/2 酸味 1 1 1 1 1 1 _ 甜味 1 >/2 1 >/2 1 !/2 1 % 1 '/2 1 % 鮮味 1 1 1 1 1 酸的酸味 1 ^2 2 2 2 2 木頭味/木屑味 1 % 1 */2 1】/2 1 >/2 1 */2 乾燥 1 1 1 1 1 1 粉末狀口感 1 '/2 1 y2 1 */2 1 >/2 1 */2 粗砂質口感 2 生青味/豆味/乾豆味 2 外觀: 不透明 不透明 不透明 不透明 不透明 不透明 黃褐色 黃褐色 黃褐色 黃褐色 黃褐色 黃褐色 --—_____ 及灰色 及灰色 及灰色 及灰色 及灰色 及灰色 實例5-34 貫例5-34說明本發明之各種嬰兒配方營養粉,其包括豌 豆蛋白水解物作為蛋白質之至少一個來源,且在一些情況 下作為蛋白質之唯一來源,其成分列於下表中。豌豆蛋白 ^解物之水解度(經調整)為約7.0%至小於19.0%。藉由各 種方法以各別批次製備此等產品,在使狀前用水復原至 :=¼成分濃度。以下所述之包括豌豆蛋白水解物作為 之唯一來源的實施例為低過敏性實施例。 i58197.doc • 44 - 201212828 實例5-9The basic taste and aroma of the undigested hydrolysate in the Alimentum matrix AN* BN* CN* DN* EN* ISO* bitterness 1 1 */2 1 y2 1 % 1 */2 */2 mechanical flavor */2 y2 ' /2 '/2 »/2 '/2 sour 1 1 1 1 1 1 _ sweetness 1 >/2 1 >/2 1 !/2 1 % 1 '/2 1 % umami 1 1 1 1 1 Sour taste of acid 1 ^2 2 2 2 2 Woody taste / Sawdust 1 % 1 */2 1]/2 1 >/2 1 */2 Drying 1 1 1 1 1 1 Powdery mouthfeel 1 '/2 1 y2 1 */2 1 >/2 1 */2 coarse sandy taste 2 green taste / bean flavor / dried bean flavor 2 Appearance: opaque opaque opaque opaque opaque opaque yellow brown yellow brown yellow brown yellow brown yellow brown yellow brown - -______ and grey and grey and grey and grey and grey and grey Examples 5-34 Example 5-34 illustrates various infant formula nutritional powders of the present invention comprising pea protein hydrolysate as at least one source of protein, and in some In the case of the sole source of protein, the ingredients are listed in the table below. The degree of hydrolysis (adjusted) of the pea protein solution is from about 7.0% to less than 19.0%. These products were prepared in separate batches by various methods, and were reconstituted with water to a concentration of :1⁄4 before use. The examples described below including the pea protein hydrolysate as the sole source are hypoallergenic examples. I58197.doc • 44 - 201212828 Example 5-9
成分 每22727.3 kg之量 實例5 實例6 實例7 實例8 實例9 玉米糖漿 9091.6 kg 9091.6 kg 8925.1 kg 9091.6 kg 8758.7 kg 婉豆蛋白水解物 3329.4 kg 3162.9 kg 3495.9 kg 2996.5 kg 3662.3 kg 高油酸紅花油 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg 糖(蔗糖) 2328.6 kg 2495.1 kg 2328.6 kg 2661.5 kg 2328.6 kg 大豆油 1908.3 kg 1908.3 kg 1908.3 kg 1908.3 kg 1908.3 kg 椰子油 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 5%氫氧化鉀 600 kg 600 kg 600 kg 600 kg 600 kg 檸檬酸卸#1、#2 279.8 kg 279.8 kg 279.8 kg 279.8 kg 279.8 kg 磷酸三鈣 252.5 kg 252.5 kg 252.5 kg 252.5 kg 252.5 kg 磷酸氫鈣 147.6 kg 147.6 kg 147.6 kg 147.6 kg 147.6 kg 氯化納 76.2 kg 76.2 kg 76.2 kg 76.2 kg 76.2 kg 氯化鎂 58.8 kg 58.8 kg 58.8 kg 58.8 kg 58.8 kg L-甲硫胺酸 36.2 kg 36.2 kg 36.2 kg 36.2 kg 36.2 kg 抗壞血酸 36.1 kg 36.1 kg 36.1 kg 36.1 kg 36.1 kg 維生素/礦物質/胺基乙 磺酸預混物 32.5 kg 32.5 kg 32.5 kg 32.5 kg 32.5 kg 氯化膽鹼 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg 維生素A、D3、E及K1 預混物 8.7 kg 8.7 kg 8.7 kg 8.7 kg 8.7 kg 棕櫊酸抗壞血酸酯 8.1kg 8.1 kg 8.1 kg 8.1 kg 8.1kg 硫酸亞鐵 6.9 kg 6.9 kg 6.9 kg 6.9 kg 6.9 kg 氯化鉀 4.3 kg 4.3 kg 4.3 kg 4.3 kg 4.3 kg 混合生育酚 3.7 kg 3.7 kg 3.7 kg 3.7 kg 3.7 kg L_肉驗 2.5 kg 2.5 kg 2.5 kg 2.5 kg 2.5 kg 植物油中之β-胡蘿蔔素 28.3 g 28.3 g 28.3 g 28.3 g 28.3 g 埃化鉀 22.9 g 22.9 g 22.9 g 22.9 g 22.9 g 158197.doc • 45· 201212828 實例10-14 成分 每22727.3 kg之量 實例10 實例11 實例12 實例13 實例14 玉米糖漿 9091.6 kg 9091.6 kg 9091.6 kg 9091.6 kg 9091.6 kg 婉豆蛋白水解物 2996.5 kg 2663.5 kg 2330.6 kg 1997.6 kg 1664.7 kg 大豆蛋白分離物 332.9 kg 665.9 kg 998.8 kg 1331.8 kg 1664.7 kg 高油酸紅花油 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg 糖(蔬糖) 2328.6 kg 2495.1 kg 2328.6 kg 2661.5 kg 2328.6 kg 大豆油 1908.3 kg 1908.3 kg 1908.3 kg 1908.3 kg 1908.3 kg 椰子油 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 5%氫氧化鉀 600 kg 600 kg 600 kg 600 kg 600 kg 檸檬酸鉀#1、#2 279.8 kg 279.8 kg 279.8 kg 279.8 kg 279.8 kg 磷酸三鈣 252.5 kg 252.5 kg 252.5 kg 252.5 kg 252.5 kg 磷酸氫鈣 147.6 kg 147.6 kg 147.6 kg 147.6 kg 147.6 kg 氣化鈉 76.2 kg 76.2 kg 76.2 kg 76.2 kg 76.2 kg 氣化鎂 58.8 kg 58.8 kg 58.8 kg 58.8 kg 58.8 kg L-甲硫胺酸 36.2 kg 36.2 kg 36.2 kg 36.2 kg 36.2 kg 抗壞血酸 36.1 kg 36.1 kg 36.1 kg 36.1 kg 36.1 kg 維生素/礦物質/胺基乙磺 酸預混物 32.5 kg 32.5 kg 32.5 kg 32.5 kg 32.5 kg 氣化膽鹼 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg 維生素A、D3、E及K1預 混物 8.7 kg 8.7 kg 8.7 kg 8.7 kg 8.7 kg 棕橺酸抗壞血酸酯 8.1 kg 8.1 kg 8.1kg 8.1 kg 8.1 kg 硫酸亞鐵 6.9 kg 6.9 kg 6.9 kg 6.9 kg 6.9 kg 氣化鉀 4.3 kg 4.3 kg 4.3 kg 4.3 kg 4.3 kg 混合生育酚 3.7 kg 3.7 kg 3.7 kg 3.7 kg 3.7 kg L_肉驗 2.5 kg 2.5 kg 2.5 kg 2.5 kg 2.5 kg 植物油中之β-胡蘿蔔素 28.3 g 28.3 g 28.3 g 28.3 g 28.3 g 蛾化斜 22.9 g 22.9 g 22.9 g 22.9 g 22.9 g 158197.doc -46- 201212828 實例15-19 成分 每1000 kg之量 實例15 實例16 實例17 實例18 實例19 玉米糖漿 412.6 kg 419.9 kg 405.3 kg 427.3 kg 397.9 kg 婉豆蛋白水解物 146.7 kg 139.4 kg 154.0 kg 132.0 kg 161.4 kg 高油酸紅花油 114.7 kg 114.7 kg 114.7 kg 114.7 kg 114.7 kg 蔗糖 97.9 kg 97.9 kg 97.9 kg 97.9 kg 97.9 kg 大豆油 83.9 kg 83.9 kg 83.9 kg 83.9 kg 83.9 kg 挪子油 77.3 kg 77.3 kg 77.3 kg 77.3 kg 77.3 kg 果寡醣 16.2 kg 16.2 kg 16.2 kg 16.2 kg 16,2 kg 檸檬酸鉀 15.3 kg 15.3 kg 15.3 kg 15.3 kg 15.3 kg 磷酸三鈣 11.7 kg 11.7 kg 11.7 kg 11.7 kg 11.7 kg 磷酸氫鈣 6.8 kg 6.8 kg 6.8 kg 6.8 kg 6.8 kg 氯化納 3.4 kg 3.4 kg 3.4 kg 3.4 kg 3.4 kg 花生四烯酸 2.9 kg 2.9 kg 2.9 kg 2.9 kg 2.9 kg 氣化鎂 2.6 kg 2.6 kg 2.6 kg 2.6 kg 2.6 kg L-曱硫胺酸 1.7 kg 1.7 kg 1.7 kg 1.7 kg 1.7 kg 抗壞血酸 1.6 kg 1.6 kg 1.6 kg 1.6 kg 1.6 kg 維生素/破物質/胺基乙磺酸預 混物 1.4 kg 1.4 kg 1.4 kg 1.4 kg 1.4 kg 二十二碳六烯酸 1.1 kg 1.1 kg 1.1 kg 1.1kg 1.1kg 氣化膽鹼 507.7 g 507.7 g 507.7 g 507.7 g 507.7 g 維生素ADEK預混物 372.6 g 372.6 g 372.6 g 372.6 g 372.6 g 棕櫚酸抗壞血酸酯 357.5 g 357.5 g 357.5 g 357.5 g 357.5 g 硫酸亞鐵 305.3 g 305.3 g 305.3 g 305.3 g 305.3 g 氯化卸 198.5 g 198.5 g 198.5 g 198.5 g 198.5 g 混合類胡蘿蔔素預混物 187.4g 187.4 g 187.4 g 187.4 g 187.4 g 混合生育酚 157.2 g 157.2 g 157.2 g 157.2 g 157.2 g L-肉鹼 107.8 g 107.8 g 107.8 g 107.8 g 107.8 g 块化鉀 1.0 g 1.0 g 1.0 g 1.0 g 1.0 g 5% KOH 按需要 按需要 按需要 按需要 按需要 158197.doc • 47· 201212828 實例20-24 成分 每1000 kg之量 實例20 實例21 實例22 實例23 實例24 玉米糖漿 412.6 kg 412.6 kg 412.6 kg 412.6 kg 412.6 kg 婉且蛋白水解物 132.0 kg 117.4 kg 102.7 kg 88.0 kg 73.35 kg 大豆蛋白 14.7 kg 29.3 kg 44.0 kg 58.7 kg 73.35 kg 高油酸紅花油 114.7 kg 114.7 kg 114.7 kg 114.7 kg 114.7 kg 蔗糖 97.9 kg 97.9 kg 97.9 kg 97.9 kg 97.9 kg 大豆油 83.9 kg 83.9 kg 83.9 kg 83.9 kg 83.9 kg 椰子油 77.3 kg 77.3 kg 77.3 kg 77.3 kg 77.3 kg 果募醣 16.2 kg 16.2 kg 16.2 kg 16.2 kg 16.2 kg 檸檬酸鉀 15.3 kg 15.3 kg 15.3 kg 15.3 kg 15.3 kg 磷酸三鈣 11.7 kg 11.7 kg 11.7kg 11.7kg 11.7 kg 磷酸氫鈣 6.8 kg 6.8 kg 6.8 kg 6.8 kg 6.8 kg 氣化納 3.4 kg 3.4 kg 3.4 kg 3.4 kg 3.4 kg 花生四稀酸 2.9 kg 2.9 kg 2.9 kg 2.9 kg 2.9 kg 氣化鎂 2.6 kg 2.6 kg 2.6 kg 2.6 kg 2.6 kg L-曱硫胺酸 1.7 kg 1.7 kg 1.7 kg 1.7 kg 1.7 kg 抗壞血酸 1.6 kg 16 kg 1.6 kg 1.6 kg 1.6 kg 維生素/礦物質/胺基乙磺酸預 混物 1.4 kg 1.4 kg 1.4 kg 1.4 kg 1.4 kg 二十二碳六烯酸 11 kg 1.1 kg 1.1kg 1.1 kg 1.1 kg 氣化膽鹼 507.7 g 507.7 g 507.7 g 507.7 g 507.7 g 維生素ADEK預混物 372.6 g 372.6 g 372.6 g 372.6 g 372.6 g 棕櫊酸抗壞血酸酯 357.5 g 357.5 g 357.5 g 357.5 g 357.5 g 硫酸亞鐵 305.3 g 305.3 g 305.3 g 305.3 g 305.3 g 氣化鉀 198.5 g 198.5 g 198.5 g 198.5 g 198.5 g 混合類胡蘿萄素預混物 187.4 g 187.4 g 187.4 g 187.4 g 187.4 g 混合生育酚 157.2 g 157.2 g 157.2 g 157.2 g 157.2 g L-肉驗 107.8 g 107.8 g 107.8 g 107.8 g 107.8 g 峨化奸 1.0 g l.Og l.Og l.Og l.Og 5% ΚΟΗ 按需要 按需要 按需要 按需要 按需要 158197.doc ·48· 201212828 實例25-29Ingredient per 22727.3 kg Example 5 Example 6 Example 7 Example 8 Example 9 Corn syrup 9091.6 kg 9091.6 kg 8925.1 kg 9091.6 kg 8758.7 kg Cowpea protein hydrolysate 3329.4 kg 3162.9 kg 3495.9 kg 2996.5 kg 3662.3 kg High oleic safflower oil 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg sugar (sucrose) 2328.6 kg 2495.1 kg 2328.6 kg 2661.5 kg 2328.6 kg soybean oil 1908.3 kg 1908.3 kg 1908.3 kg 1908.3 kg 1908.3 kg coconut oil 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 5% hydroxide Potassium 600 kg 600 kg 600 kg 600 kg 600 kg Citric acid unloading #1, #2 279.8 kg 279.8 kg 279.8 kg 279.8 kg 279.8 kg Tricalcium phosphate 252.5 kg 252.5 kg 252.5 kg 252.5 kg 252.5 kg Hydrogen phosphate 147.6 kg 147.6 kg 147.6 Kg 147.6 kg 147.6 kg sodium chloride 76.2 kg 76.2 kg 76.2 kg 76.2 kg 76.2 kg magnesium chloride 58.8 kg 58.8 kg 58.8 kg 58.8 kg 58.8 kg L-methionine 36.2 kg 36.2 kg 36.2 kg 36.2 kg 36.2 kg ascorbic acid 36.1 kg 36.1 kg 36.1 kg 36.1 kg 36.1 kg Vitamin/Mineral/Aminoethanesulfonic acid premix 32.5 kg 32.5 kg 32.5 kg 32.5 kg 32.5 kg Choline 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg Vitamin A, D3, E and K1 Premix 8.7 kg 8.7 kg 8.7 kg 8.7 kg 8.7 kg Ascorbyl palmitate 8.1 kg 8.1 kg 8.1 kg 8.1 kg 8.1 kg Sulfuric acid Ferrous 6.9 kg 6.9 kg 6.9 kg 6.9 kg 6.9 kg Potassium chloride 4.3 kg 4.3 kg 4.3 kg 4.3 kg 4.3 kg Mixed tocopherol 3.7 kg 3.7 kg 3.7 kg 3.7 kg 3.7 kg L_meat test 2.5 kg 2.5 kg 2.5 kg 2.5 kg 2.5 kg of β-carotene in vegetable oil 28.3 g 28.3 g 28.3 g 28.3 g 28.3 g Potassium hydride 22.9 g 22.9 g 22.9 g 22.9 g 22.9 g 158197.doc • 45· 201212828 Example 10-14 Example per 22727.3 kg 10 Example 11 Example 12 Example 13 Example 14 Corn syrup 9091.6 kg 9091.6 kg 9091.6 kg 9091.6 kg 9091.6 kg Cowpea protein hydrolysate 2996.5 kg 2663.5 kg 2330.6 kg 1997.6 kg 1664.7 kg Soy protein isolate 332.9 kg 665.9 kg 998.8 kg 1331.8 kg 1664.7 kg High oleic safflower oil 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg 2608.1 kg sugar (soybean sugar) 2328.6 kg 2495.1 kg 2328.6 kg 2661.5 kg 2328.6 kg soybean oil 1908.3 kg 1908.3 kg 1908.3 kg 1908.3 kg 1908. 3 kg coconut oil 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 1844.7 kg 5% potassium hydroxide 600 kg 600 kg 600 kg 600 kg 600 kg potassium citrate #1, #2 279.8 kg 279.8 kg 279.8 kg 279.8 kg 279.8 kg tricalcium phosphate 252.5 kg 252.5 kg 252.5 kg 252.5 kg 252.5 kg calcium hydrogen phosphate 147.6 kg 147.6 kg 147.6 kg 147.6 kg 147.6 kg gasification sodium 76.2 kg 76.2 kg 76.2 kg 76.2 kg 76.2 kg gasification magnesium 58.8 kg 58.8 kg 58.8 kg 58.8 kg 58.8 kg L -methionine 36.2 kg 36.2 kg 36.2 kg 36.2 kg 36.2 kg ascorbic acid 36.1 kg 36.1 kg 36.1 kg 36.1 kg 36.1 kg vitamin/mineral/amino ethanesulfonic acid premix 32.5 kg 32.5 kg 32.5 kg 32.5 kg 32.5 kg gas Choline 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg Vitamin A, D3, E and K1 premix 8.7 kg 8.7 kg 8.7 kg 8.7 kg 8.7 kg ascorbyl palmitate 8.1 kg 8.1 kg 8.1 kg 8.1 kg 8.1 kg sulfuric acid Ferrous 6.9 kg 6.9 kg 6.9 kg 6.9 kg 6.9 kg Gasification potassium 4.3 kg 4.3 kg 4.3 kg 4.3 kg 4.3 kg Mixed tocopherol 3.7 kg 3.7 kg 3.7 kg 3.7 kg 3.7 kg L_meat test 2.5 kg 2.5 kg 2.5 kg 2.5 kg 2.5 kg of vegetable oil in β-hu素素28.3 g 28.3 g 28.3 g 28.3 g 28.3 g moths 22.9 g 22.9 g 22.9 g 22.9 g 22.9 g 158197.doc -46- 201212828 Example 15-19 Composition per 1000 kg Example 15 Example 16 Example 17 Example 18 Example 19 Corn syrup 412.6 kg 419.9 kg 405.3 kg 427.3 kg 397.9 kg Cowpea protein hydrolysate 146.7 kg 139.4 kg 154.0 kg 132.0 kg 161.4 kg High oleic safflower oil 114.7 kg 114.7 kg 114.7 kg 114.7 kg 114.7 kg Sucrose 97.9 kg 97.9 kg 97.9 Kg 97.9 kg 97.9 kg Soybean oil 83.9 kg 83.9 kg 83.9 kg 83.9 kg 83.9 kg Necklace oil 77.3 kg 77.3 kg 77.3 kg 77.3 kg 77.3 kg Fructooligosaccharide 16.2 kg 16.2 kg 16.2 kg 16.2 kg 16,2 kg Potassium citrate 15.3 kg 15.3 kg 15.3 kg 15.3 kg 15.3 kg Tricalcium phosphate 11.7 kg 11.7 kg 11.7 kg 11.7 kg 11.7 kg Calcium hydrogen phosphate 6.8 kg 6.8 kg 6.8 kg 6.8 kg 6.8 kg Chlorinated sodium 3.4 kg 3.4 kg 3.4 kg 3.4 kg 3.4 kg Arachidene Acid 2.9 kg 2.9 kg 2.9 kg 2.9 kg 2.9 kg Magnesium 2.6 kg 2.6 kg 2.6 kg 2.6 kg 2.6 kg L-曱 thiamic acid 1.7 kg 1.7 kg 1.7 kg 1.7 kg 1.7 kg Ascorbic acid 1.6 kg 1.6 kg 1.6 kg 1.6 kg 1.6 Kg live Prime/breaking substance/amino ethanesulfonic acid premix 1.4 kg 1.4 kg 1.4 kg 1.4 kg 1.4 kg docosahexaenoic acid 1.1 kg 1.1 kg 1.1 kg 1.1 kg 1.1 kg gasified choline 507.7 g 507.7 g 507.7 g 507.7 g 507.7 g Vitamin ADEK premix 372.6 g 372.6 g 372.6 g 372.6 g 372.6 g ascorbyl palmitate 357.5 g 357.5 g 357.5 g 357.5 g 357.5 g ferrous sulfate 305.3 g 305.3 g 305.3 g 305.3 g 305.3 g Chlorination unloading 198.5 g 198.5 g 198.5 g 198.5 g 198.5 g Mixed carotenoid premix 187.4g 187.4 g 187.4 g 187.4 g 187.4 g Mixed tocopherol 157.2 g 157.2 g 157.2 g 157.2 g 157.2 g L-carnitine 107.8 g 107.8 g 107.8 g 107.8 g 107.8 g Potassium Potassium 1.0 g 1.0 g 1.0 g 1.0 g 1.0 g 5% KOH As needed, as needed, as needed 158197.doc • 47· 201212828 Example 20-24 Composition per 1000 kg Example 20 Example 21 Example 22 Example 23 Example 24 Corn syrup 412.6 kg 412.6 kg 412.6 kg 412.6 kg 412.6 kg 蛋白 Protein hydrolysate 132.0 kg 117.4 kg 102.7 kg 88.0 kg 73.35 kg Soy protein 14.7 kg 29.3 kg 44.0 kg 58.7 kg 73.35 kg High oil Safflower oil 114.7 kg 114.7 kg 114.7 kg 114.7 kg 114.7 kg sucrose 97.9 kg 97.9 kg 97.9 kg 97.9 kg 97.9 kg soybean oil 83.9 kg 83.9 kg 83.9 kg 83.9 kg 83.9 kg coconut oil 77.3 kg 77.3 kg 77.3 kg 77.3 kg 77.3 kg fruit sugar 16.2 kg 16.2 kg 16.2 kg 16.2 kg 16.2 kg Potassium citrate 15.3 kg 15.3 kg 15.3 kg 15.3 kg 15.3 kg Tricalcium phosphate 11.7 kg 11.7 kg 11.7 kg 11.7 kg 11.7 kg Calcium hydrogen phosphate 6.8 kg 6.8 kg 6.8 kg 6.8 kg 6.8 kg gas 3.4 kg 3.4 kg 3.4 kg 3.4 kg 3.4 kg peanut tetrahydro acid 2.9 kg 2.9 kg 2.9 kg 2.9 kg 2.9 kg gasification magnesium 2.6 kg 2.6 kg 2.6 kg 2.6 kg 2.6 kg L-antimony citrate 1.7 kg 1.7 kg 1.7 Kg 1.7 kg 1.7 kg Ascorbic acid 1.6 kg 16 kg 1.6 kg 1.6 kg 1.6 kg Vitamin/Mineral/Aminoethanesulfonic acid premix 1.4 kg 1.4 kg 1.4 kg 1.4 kg 1.4 kg docosahexaenoic acid 11 kg 1.1 kg 1.1kg 1.1 kg 1.1 kg gas choline 507.7 g 507.7 g 507.7 g 507.7 g 507.7 g vitamin ADEK premix 372.6 g 372.6 g 372.6 g 372.6 g 372.6 g palmitate ascorbate 357.5 g 357.5 g 357.5 g 357.5 g 357.5 g Ferrous sulfate 305.3 g 3 05.3 g 305.3 g 305.3 g 305.3 g potassium carbonate 198.5 g 198.5 g 198.5 g 198.5 g 198.5 g mixed carotenoid premix 187.4 g 187.4 g 187.4 g 187.4 g 187.4 g mixed tocopherol 157.2 g 157.2 g 157.2 g 157.2 g 157.2 g L-meat test 107.8 g 107.8 g 107.8 g 107.8 g 107.8 g 峨 rape 1.0 g l.Og l.Og l.Og l.Og 5% ΚΟΗ As needed, as needed, as needed 158197.doc ·48· 201212828 Example 25-29
成分 每22679.62 kg之量 實例25 實例26 實例27 實例28 實例29 麥芽糊精 7595 kg 7793.9 kg 7396.1 kg 7992.8 kg 7197.2 kg 婉豆蛋白水解物 3978 kg 3779.1 kg 4176.9 kg 3580.2 kg 4375.8 kg 蔗糖 3288 kg 3288 kg 3288 kg 3288 kg 3288 kg 高油酸紅花油 2204 kg 2204 kg 2204 kg 2204 kg 2204 kg 分餾椰子油(MCT) 2162 kg 2162 kg 2162 kg 2162 kg 2162 kg 大豆油 1825 kg 1825 kg 1825 kg 1825 kg 1825 kg 微米尺寸化磷酸三鈣 329 kg 329 kg 329 kg 329 kg 329 kg Panodan FDPK 326 kg 326 kg 326 kg 326 kg 326 kg 擰檬酸鉀 276 kg 276 kg 276 kg 276 kg 276 kg 超細三仙膠 128 kg 128 kg 128 kg 128 kg 128 kg 氯化鎂 69.6 kg 69.6 kg 69.6 kg 69.6 kg 69.6 kg 蒸餾單酸甘油酯 65.2 kg 65.2 kg 65.2 kg 65.2 kg 65.2 kg 花生四烯酸(AA) 60.0 kg 60.0 kg 60.0 kg 60.0 kg 60.0 kg 氣化鈉 59.2 kg 59.2 kg 59.2 kg 59.2 kg 59.2 kg 抗壞血酸 51.2 kg 51.2 kg 51.2 kg 51.2 kg 51.2 kg L-胱胺酸二鹽酸鹽 46.6 kg 46.6 kg 46.6 kg 46.6 kg 46.6 kg 碳麟 33.7 kg 33.7 kg 33.7 kg 33.7 kg 33.7 kg 維生素/礦物質/胺基乙磺 酸預混物 31.1 kg 31.1kg 31.1kg 31.1kg .31.1 kg 二十二碳六稀酸(DHA) 22.5 kg 22.5 kg 22.5 kg 22.5 kg 22.5 kg L-酪胺酸 19.7 kg 19.7 kg 19.7 kg 19.7 kg 19.7 kg 氣化鉀 16.7 kg 16.7 kg 16.7 kg 16.7 kg 16.7 kg 158197.doc -49- 201212828 氣化膽鹼 13.0 kg 13.0 kg 13.0 kg 13.0 kg 13.0 kg L-色胺酸 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg 硫酸亞鐵 10.9 kg 10.9 kg 10.9 kg 10.9 kg 10.9 kg 維生素A、D3、E及K1預 混物 8.53 kg 8.53 kg 8.53 kg 8.53 kg 8.53 kg 棕櫚酸抗壞血酸酯 5.34 kg 5.34 kg 5.34 kg 5.34 kg 5.34 kg 生育酚-2食品級抗氧化劑 2.67 kg 2.67 kg 2.67 kg 2.67 kg 2.67 kg L-肉鹼 2.52 kg 2.52 kg 2.52 kg 2.52 kg 2.52 kg 破化钟 33.8 g 33.8 g 33.8 g 33.8 g 33.8 g 5%氫氧化鉀(加工助劑) 按需要 按需要 按需要 按需要 按需要 實例30-34 成分 每22679.62 kg之量 實例30 實例31 實例32 實例33 實例34 麥芽糊精 7595 kg 7595 kg 7595 kg 7595 kg 7595 kg 婉豆蛋白水解物 3580.2 kg 3182.4 kg 2784.6 kg 2386.8 kg 1989 kg 路蛋白水解物 397.8 kg 795.6 kg 1193.4 kg 1591.2 kg 1989 kg 蔗糖 3288 kg 3288 kg 3288 kg 3288 kg 3288 kg 高油酸紅花油 2204 kg 2204 kg 2204 kg 2204 kg 2204 kg 分餾椰子油(MCT) 2162 kg 2162 kg 2162 kg 2162 kg 2162 kg 大豆油 1825 kg 1825 kg 1825 kg 1825 kg 1825 kg 微米尺寸化磷酸三鈣 329 kg 329 kg 329 kg 329 kg 329 kg Panodan FDPK 326 kg 326 kg 326 kg 326 kg 326 kg 檸檬酸鉀 276 kg 276 kg 276 kg 276 kg 276 kg 超細三仙膠 128 kg 128 kg 128 kg 128 kg 128 kg 氣化鎂 69.6 kg 69.6 kg 69.6 kg 69.6 kg 69.6 kg 蒸傑單酸甘油醋 65.2 kg 65.2 kg 65.2 kg 65.2 kg 65.2 kg 花生四烯酸(AA) 60.0 kg 60.0 kg 60.0 kg 60.0 kg 60.0 kg 158197.doc -50- 201212828 氯化納 59.2 kg 59.2 kg 59.2 kg 59.2 kg 59.2 kg 抗壞血酸 51.2 kg 51.2 kg 51.2 kg 51.2 kg 51.2 kg L-胱胺酸二鹽酸鹽 46.6 kg 46.6 kg 46.6 kg 46.6 kg 46.6 kg 碳酸鈣 33.7 kg 33.7 kg 33.7 kg 33.7 kg 33.7 kg 維生素/礦物質/胺基乙磺 酸預混物 31.1 kg 31.1 kg 31.1 kg 31.1kg 31.1kg 二十二破六稀酸(DHA) 22.5 kg 22.5 kg 22.5 kg 22.5 kg 22.5 kg L-酪胺酸 19.7 kg 19.7 kg 19.7 kg 19.7 kg 19.7 kg 氯化鉀 16.7 kg 16.7 kg 16.7 kg 16.7 kg 16.7 kg 氯化膽驗 13.0 kg 13.0 kg 13.0 kg 13.0 kg 13.0 kg L-色胺酸 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg 硫酸亞鐵 10.9 kg 10.9 kg 10.9 kg 10.9 kg 10.9 kg 維生素A、D3、E及K1預 混物 8.53 kg 8.53 kg 8.53 kg 8.53 kg 8.53 kg 棕櫊酸抗壞血酸酯 5.34 kg 5.34 kg 5.34 kg 5.34 kg 5.34 kg 生育酚-2食品級抗氧化劑 2.67 kg 2.67 kg 2.67 kg 2.67 kg 2.67 kg L-肉驗 2.52 kg 2.52 kg 2.52 kg 2.52 kg 2.52 kg 蛾化卸 33.8 g 33.8 g 33.8 g 33.8 g 33.8 g 5%氫氧化錦(加工助劑) 按需要 按需要 按需要 按需要 按需要 實例35-54 實例35-54說明本發明之營養乳液實施例,其包括豌豆 蛋白水解物作為蛋白質之至少一個來源,且在一些情況下 作為蛋白質之唯一來源,其成分列於下表中。為製備營養 乳液,製備至少三種各別漿料(例如CHO-MIN漿料、PIW 漿料、PIF漿料),接著摻合在一起,熱處理,均質化並標 準化。將所得組合物無菌包裝至塑膠瓶中或蒸餾滅菌。以 下所述之包括豌豆蛋白水解物作為蛋白質之唯一來源的實 158197.doc -51 - 201212828 施例為低過敏性實施例。 實例35-39 成分 每1000 Kg之量 實例35 實例36 實例37 實例38 實例39 成分水 足量 足量 足量 足量 足量 玉米糖漿 63.5 kg 63.5 kg 63.5 kg 63.5 kg 63.5 kg 婉豆蛋白水解物 18.9 kg 17.9 kg 19.8 kg 17.0 kg 20.8 kg 高油酸紅花油 14.0 kg 14.0 kg 14.0 kg 14.0 kg 14.0 kg 蔗糖 12.6 kg 12.6 kg 12.6 kg 12.6 kg 12.6 kg 大豆油 10.5 kg 10.5 kg 10.5 kg 10.5 kg 10.5 kg 椰子油 10.0 kg 10.0 kg 10.0 kg 10.0 kg 10.0 kg 果寡餹 2.3 kg 2.3 kg 2.3 kg 2.3 kg 2.3 kg 檸檬酸鈣 1.7 kg 1.7 kg 1.7 kg 1.7 kg 1.7 kg 細微磷酸三鈣 1.1 kg 1.1 kg 1.1 kg 1.1 kg 1.1 kg 磷酸二氫鉀 744.4 g 744.4 g 744.4 g 744.4 g 744.4 g 抗壞血酸 437.2 g 437.2 g 437.2 g 437.2 g 437.2 g 檸檬酸鉀 406.0 g 406.0 g 406.0 g 406.0 g 406.0 g 氣化鎂 373.4 373.4 373.4 373.4 373.4 花生四烯酸油 366.4 g 366.4 g 366.4 g 366.4 g 366.4 g 單酸甘油酯 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g 卵磷脂 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g 氣化納 309.5 g 309.5 g 309.5 g 309.5 g 309.5 g 磷酸氫二鉀 237.9 g 237.9 g 237.9 g 237.9 g 237.9 g L-甲硫胺酸 208.0 g 208.0 g 208.0 g 208.0 g 208.0 g Seakem RLC 200.1 g 200.1 g 200.1 g 200.1 g 200.1 g 維生素/礦物質/胺基乙磺酸 預混物 176.4 g 176.4 g 176.4 g 176.4 g 176.4 g 氣化:if 143.6 g 143.6 g 143.6 g 143.6 g 143.6 g 二十二碳六烯酸油 135.4 g 135.4 g 135.4 g 135.4 g 135.4 g 158197.doc -52- 201212828 氯化膽鹼 71.3 g 71.3 g 71.3 g 71.3 g 71.3 g 維生素ADEK預混物 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g 硫酸亞鐵 44.8 g 44.8 g 44.8 g 44.8 g 44.8 g 混合類胡蘿蔔素預混物 26.5 g 26.5 g 26.5 g 26.5 g 26.5 g L-肉鹼 13.8 g 13.8 g 13.8 g 13.8 g 13.8 g 核黃素 2.0 g 2.0 g 2.0 g 2.0 g 2.0 g 維生素A棕櫚酸酯 780.0 mg 780.0 mg 780.0 mg 780.0 mg 780.0 mg 峨化鉀 150.0 mg 150.0 mg 150.0 mg 150.0 mg 150.0 mg 5% KOH 按需要 按需要 按需要 按需要 按需要 實例40-44Composition per 22679.62 kg Example 25 Example 26 Example 27 Example 28 Example 29 Maltodextrin 7595 kg 7793.9 kg 7396.1 kg 7992.8 kg 7197.2 kg Cowpea protein hydrolysate 3978 kg 3779.1 kg 4176.9 kg 3580.2 kg 4375.8 kg Sucrose 3288 kg 3288 kg 3288 kg 3288 kg 3288 kg High oleic safflower oil 2204 kg 2204 kg 2204 kg 2204 kg 2204 kg Fractionated coconut oil (MCT) 2162 kg 2162 kg 2162 kg 2162 kg 2162 kg Soybean oil 1825 kg 1825 kg 1825 kg 1825 kg 1825 kg Micron Digested Tricalcium Phosphate 329 kg 329 kg 329 kg 329 kg 329 kg Panodan FDPK 326 kg 326 kg 326 kg 326 kg 326 kg Potassium citrate 276 kg 276 kg 276 kg 276 kg 276 kg Superfine Sanxian gel 128 kg 128 kg 128 kg 128 kg 128 kg Magnesium chloride 69.6 kg 69.6 kg 69.6 kg 69.6 kg 69.6 kg Distilled monoglyceride 65.2 kg 65.2 kg 65.2 kg 65.2 kg 65.2 kg Arachidonic acid (AA) 60.0 kg 60.0 kg 60.0 kg 60.0 kg 60.0 kg Gas Sodium 59.2 kg 59.2 kg 59.2 kg 59.2 kg 59.2 kg Ascorbic acid 51.2 kg 51.2 kg 51.2 kg 51.2 kg 51.2 kg L-cystine dihydrochloride 46.6 kg 46.6 kg 46.6 kg 46.6 kg 46.6 kg Carbon Lin 33.7 Kg 33.7 kg 33.7 kg 33.7 kg 33.7 kg Vitamin/Mineral/Aminoethanesulfonic acid premix 31.1 kg 31.1kg 31.1kg 31.1kg .31.1 kg Teflon acid (DHA) 22.5 kg 22.5 kg 22.5 kg 22.5 Kg 22.5 kg L-tyramine 19.7 kg 19.7 kg 19.7 kg 19.7 kg 19.7 kg Gasification potassium 16.7 kg 16.7 kg 16.7 kg 16.7 kg 16.7 kg 158197.doc -49- 201212828 Gasification choline 13.0 kg 13.0 kg 13.0 kg 13.0 kg 13.0 kg L-Tryptophan 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg Ferrous sulfate 10.9 kg 10.9 kg 10.9 kg 10.9 kg 10.9 kg Vitamin A, D3, E and K1 premix 8.53 kg 8.53 kg 8.53 kg 8.53 kg 8.53 Kg palmitate ascorbate 5.34 kg 5.34 kg 5.34 kg 5.34 kg 5.34 kg tocopherol-2 food grade antioxidant 2.67 kg 2.67 kg 2.67 kg 2.67 kg 2.67 kg L-carnitine 2.52 kg 2.52 kg 2.52 kg 2.52 kg 2.52 kg Broken clock 33.8 g 33.8 g 33.8 g 33.8 g 33.8 g 5% potassium hydroxide (processing aid) As needed, as needed, as needed, examples 30-34, ingredients per 22679.62 kg, examples 30, examples 31, examples 32, examples 33, examples 34 Maltodextrin 7595 kg 7595 kg 7595 kg 75 95 kg 7595 kg cowpea protein hydrolysate 3580.2 kg 3182.4 kg 2784.6 kg 2386.8 kg 1989 kg road protein hydrolysate 397.8 kg 795.6 kg 1193.4 kg 1591.2 kg 1989 kg sucrose 3288 kg 3288 kg 3288 kg 3288 kg 3288 kg high oleic safflower oil 2204 Kg 2204 kg 2204 kg 2204 kg 2204 kg Fractionated coconut oil (MCT) 2162 kg 2162 kg 2162 kg 2162 kg 2162 kg Soybean oil 1825 kg 1825 kg 1825 kg 1825 kg 1825 kg Micronized tricalcium phosphate 329 kg 329 kg 329 kg 329 Kg 329 kg Panodan FDPK 326 kg 326 kg 326 kg 326 kg 326 kg potassium citrate 276 kg 276 kg 276 kg 276 kg 276 kg ultrafine three scented plastic 128 kg 128 kg 128 kg 128 kg 128 kg gasified magnesium 69.6 kg 69.6 kg 69.6 kg 69.6 kg 69.6 kg steamed monoglyceride 65.2 kg 65.2 kg 65.2 kg 65.2 kg 65.2 kg arachidonic acid (AA) 60.0 kg 60.0 kg 60.0 kg 60.0 kg 60.0 kg 158197.doc -50- 201212828 sodium chloride 59.2 Kg 59.2 kg 59.2 kg 59.2 kg 59.2 kg Ascorbic acid 51.2 kg 51.2 kg 51.2 kg 51.2 kg 51.2 kg L-cystine dihydrochloride 46.6 kg 46.6 kg 46.6 kg 46.6 kg 46.6 kg Calcium carbonate 33.7 kg 33.7 kg 33.7 kg 33.7 kg 3 3.7 kg Vitamin/Mineral/Aminoethanesulfonic acid premix 31.1 kg 31.1 kg 31.1 kg 31.1kg 31.1kg Twenty-two broken hexahydrate (DHA) 22.5 kg 22.5 kg 22.5 kg 22.5 kg 22.5 kg L-tyramine 19.7 kg 19.7 kg 19.7 kg 19.7 kg 19.7 kg Potassium chloride 16.7 kg 16.7 kg 16.7 kg 16.7 kg 16.7 kg Chloride test 13.0 kg 13.0 kg 13.0 kg 13.0 kg 13.0 kg L-tryptophan 11.5 kg 11.5 kg 11.5 kg 11.5 kg 11.5 kg ferrous sulfate 10.9 kg 10.9 kg 10.9 kg 10.9 kg 10.9 kg Vitamin A, D3, E and K1 premix 8.53 kg 8.53 kg 8.53 kg 8.53 kg 8.53 kg Palmitic acid ascorbate 5.34 kg 5.34 kg 5.34 kg 5.34 kg 5.34 Kg tocopherol-2 food grade antioxidant 2.67 kg 2.67 kg 2.67 kg 2.67 kg 2.67 kg L-meat test 2.52 kg 2.52 kg 2.52 kg 2.52 kg 2.52 kg moth release 33.8 g 33.8 g 33.8 g 33.8 g 33.8 g 5% hydroxide Brocade (Processing Aid) As needed, as needed, Examples 35-54, as needed, Examples 35-54 illustrate an embodiment of a nutritional emulsion of the present invention comprising pea protein hydrolysate as at least one source of protein, and in some cases The only one as a protein Sources, whose ingredients are listed in the table below. To prepare the nutritional emulsion, at least three separate slurries (e.g., CHO-MIN slurries, PIW slurries, PIF slurries) are prepared, then blended together, heat treated, homogenized, and standardized. The resulting composition is aseptically packaged into a plastic bottle or sterilized by distillation. The following includes the pea protein hydrolysate as the sole source of protein. 158197.doc -51 - 201212828 The example is a hypoallergenic example. Example 35-39 Ingredient per 1000 Kg Example 35 Example 36 Example 37 Example 38 Example 39 Ingredients sufficient in water sufficient sufficient amount of corn syrup 63.5 kg 63.5 kg 63.5 kg 63.5 kg 63.5 kg cowpea protein hydrolysate 18.9 Kg 17.9 kg 19.8 kg 17.0 kg 20.8 kg High oleic acid safflower oil 14.0 kg 14.0 kg 14.0 kg 14.0 kg 14.0 kg Sucrose 12.6 kg 12.6 kg 12.6 kg 12.6 kg 12.6 kg Soybean oil 10.5 kg 10.5 kg 10.5 kg 10.5 kg 10.5 kg Coconut oil 10.0 Kg 10.0 kg 10.0 kg 10.0 kg 10.0 kg Fructus bismuth 2.3 kg 2.3 kg 2.3 kg 2.3 kg 2.3 kg Calcium citrate 1.7 kg 1.7 kg 1.7 kg 1.7 kg 1.7 kg Tricalcium phosphate 1.1 kg 1.1 kg 1.1 kg 1.1 kg 1.1 kg Phosphoric acid Potassium dihydrogen 744.4 g 744.4 g 744.4 g 744.4 g 744.4 g ascorbic acid 437.2 g 437.2 g 437.2 g 437.2 g 437.2 g potassium citrate 406.0 g 406.0 g 406.0 g 406.0 g 406.0 g Magnesium sulphate 373.4 373.4 373.4 373.4 373.4 Arachidonic acid 366.4 g 366.4 g 366.4 g 366.4 g 366.4 g monoglyceride 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g lecithin 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g gasification nano 309.5 g 3 09.5 g 309.5 g 309.5 g 309.5 g dipotassium hydrogen phosphate 237.9 g 237.9 g 237.9 g 237.9 g 237.9 g L-methionine 208.0 g 208.0 g 208.0 g 208.0 g 208.0 g Seakem RLC 200.1 g 200.1 g 200.1 g 200.1 g 200.1 g Vitamin/Mineral/Aminoethanesulfonic acid premix 176.4 g 176.4 g 176.4 g 176.4 g 176.4 g Gasification: if 143.6 g 143.6 g 143.6 g 143.6 g 143.6 g docosahexaenoic acid 135.4 g 135.4 g 135.4 g 135.4 g 135.4 g 158197.doc -52- 201212828 Choline chloride 71.3 g 71.3 g 71.3 g 71.3 g 71.3 g Vitamin ADEK premix 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g Ferrous sulfate 44.8 g 44.8 g 44.8 g 44.8 g 44.8 g Mixed carotenoid premix 26.5 g 26.5 g 26.5 g 26.5 g 26.5 g L-carnitine 13.8 g 13.8 g 13.8 g 13.8 g 13.8 g Riboflavin 2.0 g 2.0 g 2.0 g 2.0 g 2.0 g Vitamin A Palmitate 780.0 mg 780.0 mg 780.0 mg 780.0 mg 780.0 mg Potassium Bismuth 150.0 mg 150.0 mg 150.0 mg 150.0 mg 150.0 mg 5% KOH As needed, as needed, as needed, 40-44
成分 每1000 Kg之量 實例40 實例41 實例42 實例43 實例44 成分水 足量 足量 足量 足量 足量 玉米糖漿 63.5 kg 63.5 kg 63.5 kg 63.5 kg 63.5 kg 婉豆蛋白水解物 17.0 kg 15.1 kg 13.2 kg 11.3 kg 9.45 kg 大豆蛋白 1.9 kg 3.9 kg 5.8 kg 7.7 kg 9.45 kg 高油酸紅花油 14.0 kg 14.0 kg 14.0 kg 14.0 kg 14.0 kg 蔗糖 12.6 kg 12.6 kg 12.6 kg 12.6 kg 12.6 kg 大豆油 10.5 kg 10.5 kg 10.5 kg 10.5 kg 10.5 kg 椰子油 10.0 kg 10.0 kg 10.0 kg 10.0 kg 10,0 kg 果寡醣 2.3 kg 2.3 kg 2.3 kg 2.3 kg 2.3 kg 擰檬酸鈣 1.7 kg 1.7 kg 1.7 kg 1.7 kg 1.7 kg 細微磷酸三鈣 1.1 kg 1.1 kg 1.1 kg 1.1 kg 1.1kg 麟酸二氫鉀 744.4 g 744.4 g 744.4 g 744.4 g 744.4 g 抗壞血酸 437.2 g 437.2 g 437.2 g 437.2 g 437.2 g 檸檬酸鉀 406.0 g 406.0 g 406.0 g 406.0 g 406.0 g 氯化鎂 373.4 373.4 373.4 373.4 373.4 花生四烯酸油 366.4 g 366.4 g 366.4 g 366.4 g 366.4 g 158197.doc -53- 201212828 單酸甘油西旨 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g 卵磷脂 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g 氣化鈉 309.5 g 309.5 g 309.5 g 309.5 g 309.5 g 磷酸氫二鉀 237.9 g 237.9 g 237.9 g 237.9 g 237.9 g L-曱硫胺酸 208.0 g 208.0 g 208.0 g 208.0 g 208.0 g Seakem RLC 200.1 g 200.1 g 200.1 g 200.1 g 200.1 g 維生素/礦物質/胺基乙磺酸 預混物 176.4 g 176.4 g 176.4 g 176.4 g 176.4 g 氣化鉀 143.6 g 143.6 g 143.6 g 143.6 g 143.6 g 二十二破六烯酸油 135.4 g 135.4 g 135.4 g 135.4 g 135.4 g 氣化膽鹼 71.3 g 71.3 g 71.3 g 71.3 g 71.3 g 維生素ADEK預混物 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g 硫酸亞鐵 44.8 g 44.8 g 44.8 g 44.8 g 44.8 g 混合類胡蘿蔔素預混物 26.5 g 26.5 g 26.5 g 26.5 g 26.5 g L-肉驗 13.8 g 13.8 g 13.8 g 13.8 g 13.8 g 核黃素 2.0 g 2.0 g 2.0 g 2.0 g 2.0 g 維生素A棕櫊酸酯 780.0 mg 780.0 mg 780.0 mg 780.0 mg 780.0 mg 蛾化鉀 150.0 mg 150.0 mg 150.0 mg 150.0 mg 150.0 mg 5% KOH 按需要 按需要 按需要 按需要 按需要 實例45-49 成分 每45359.24 kg之量 實例45 實例46 實例47 實例48 實例49 成分水 足量 足量 足量 足量 足量 蔗糖 2007 kg 2007 kg 2007 kg 2007 kg 2007 kg 婉豆蛋白水解物 1025 kg 973.8 kg 1076.2 kg 922.5 kg 1127.5 kg 改質木薯澱粉 989 kg 989 kg 989 kg 989 kg 989 kg 紅花油 644 kg 644 kg 644 kg 644 kg 644 kg 158197.doc -54- 201212828Ingredient per 1000 Kg Example 40 Example 41 Example 42 Example 43 Example 44 Ingredients sufficient in water sufficient in sufficient amount sufficient corn syrup 63.5 kg 63.5 kg 63.5 kg 63.5 kg 63.5 kg cowpea protein hydrolysate 17.0 kg 15.1 kg 13.2 Kg 11.3 kg 9.45 kg Soy protein 1.9 kg 3.9 kg 5.8 kg 7.7 kg 9.45 kg High oleic acid safflower oil 14.0 kg 14.0 kg 14.0 kg 14.0 kg 14.0 kg Sucrose 12.6 kg 12.6 kg 12.6 kg 12.6 kg 12.6 kg Soybean oil 10.5 kg 10.5 kg 10.5 Kg 10.5 kg 10.5 kg Coconut oil 10.0 kg 10.0 kg 10.0 kg 10.0 kg 10,0 kg Fructooligosaccharide 2.3 kg 2.3 kg 2.3 kg 2.3 kg 2.3 kg Calcium citrate 1.7 kg 1.7 kg 1.7 kg 1.7 kg 1.7 kg Tricalcium phosphate 1.1 kg 1.1 kg 1.1 kg 1.1 kg 1.1kg Potassium dihydrogenate 744.4 g 744.4 g 744.4 g 744.4 g 744.4 g Ascorbic acid 437.2 g 437.2 g 437.2 g 437.2 g 437.2 g Potassium citrate 406.0 g 406.0 g 406.0 g 406.0 g 406.0 g Magnesium chloride 373.4 373.4 373.4 373.4 373.4 Arachidonic acid oil 366.4 g 366.4 g 366.4 g 366.4 g 366.4 g 158197.doc -53- 201212828 Monoglyceride West 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g Egg Grease 350.0 g 350.0 g 350.0 g 350.0 g 350.0 g sodium vaporate 309.5 g 309.5 g 309.5 g 309.5 g 309.5 g dipotassium hydrogen phosphate 237.9 g 237.9 g 237.9 g 237.9 g 237.9 g L-oxime thioglycol 208.0 g 208.0 g 208.0 g 208.0 g 208.0 g Seakem RLC 200.1 g 200.1 g 200.1 g 200.1 g 200.1 g Vitamin/Mineral/Aminoethanesulfonic acid premix 176.4 g 176.4 g 176.4 g 176.4 g 176.4 g Gasification potassium 143.6 g 143.6 g 143.6 g 143.6 g 143.6 g dihydropentaenoic acid oil 135.4 g 135.4 g 135.4 g 135.4 g 135.4 g gasified choline 71.3 g 71.3 g 71.3 g 71.3 g 71.3 g vitamin ADEK premix 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g sulfuric acid Ferrous 44.8 g 44.8 g 44.8 g 44.8 g 44.8 g Mixed carotenoid premix 26.5 g 26.5 g 26.5 g 26.5 g 26.5 g L-meat 13.8 g 13.8 g 13.8 g 13.8 g 13.8 g Riboflavin 2.0 g 2.0 g 2.0 g 2.0 g 2.0 g Vitamin A palmitate 780.0 mg 780.0 mg 780.0 mg 780.0 mg 780.0 mg Potassium moth 150.0 mg 150.0 mg 150.0 mg 150.0 mg 150.0 mg 5% KOH As needed, as needed -49 ingredients per 45359.24 kg Example 45 Example 46 Example 47 Example 48 Example 49 Ingredients in water sufficient in sufficient amount sufficient sucrose 2007 kg 2007 kg 2007 kg 2007 kg 2007 kg cowpea protein hydrolysate 1025 kg 973.8 kg 1076.2 kg 922.5 kg 1127.5 kg Tapioca starch 989 kg 989 kg 989 kg 989 kg 989 kg safflower oil 644 kg 644 kg 644 kg 644 kg 644 kg 158197.doc -54- 201212828
分餾椰子油 568 kg 568 kg 568 kg 568 kg 568 kg 大豆油 479 kg 479 kg 479 kg 479 kg 479 kg 5% KOH(加工助劑) 187 kg 187 kg 187 kg 187 kg 187 kg 檸檬酸鈣 69.9 kg 69.9 kg 69.9 kg 69.9 kg 69.9 kg 磷酸氫鈣 48.1 kg 48.1 kg 48.1 kg 48.1 kg 48.1 kg 角叉菜膠 45.4 kg 45.4 kg 45.4 kg 45.4 kg 45.4 kg 磷酸氫二鉀 26.2 kg 26.2 kg 26.2 kg 26.2 kg 26.2 kg 抗壞血酸 24.2 kg 24.2 kg 24.2 kg 24.2 kg 24.2 kg 氯化鎂 18.6 kg 18.6 kg 18.6 kg 18.6 kg 18.6 kg 高山被抱黴(M. Alpina)油 (ARA 油) 17.9 kg 17.9 kg 17.9 kg 17.9 kg 17.9 kg 檸檬酸鉀 15.9 kg 15.9 kg 15.9 kg 15.9 kg 15.9 kg 氣化納 14.9 kg 14.9 kg 14.9 kg 14.9 kg 14.9 kg 氫氧化#5 14.0 kg 14.0 kg 14.0 kg 14.0 kg 14.0 kg L-胱胺酸二鹽酸鹽 12.1 kg 12.1 kg 12.1 kg 12.1 kg 12.1 kg 氣化鉀 11.2 kg 11.2 kg 11.2 kg 11.2 kg 11.2 kg 水溶性維生素預混物 8.00 kg 8.00 kg 8.00 kg 8.00 kg 8.00 kg 藻類隱曱藻(C. 0?;2响)油 (DHA 油) 6.71 kg 6.71 kg 6.71 kg 6.71 kg 6.71 kg L-酪胺酸 5.08 kg 5.08 kg 5.08 kg 5.08 kg 5.08 kg L-色胺酸 4.67 kg 4.67 kg 4.67 kg 4.67 kg 4.67 kg 氣化膽驗 3.36 kg 3.36 kg 3.36 kg 3.36 kg 3.36 kg 油溶性維生素預混物 2.37 kg 2.37 kg 2.37 kg 2.37 kg 2.37 kg 硫酸亞鐵 2.32 kg 2.32 kg 2.32 kg 2.32 kg 2.32 kg L-肉驗 650 g 650 g 650 g 650 g 650 g 核黃素 87.9 g 87.9 g 87.9 g 87.9 g 87.9 g 維生素A棕櫊酸酯 17.6 g 17.6 g 17.6 g 17.6 g 17.6 g 破化鉀 8.70 g 8.70 g 8.70 g 8.70 g 8.70 g 158197.doc -55- 201212828 實例50-54 成分 每45359.24 kg之量 實例50 實例51 實例52 實例53 實例54 成分水 足量 足量 足量 足量 足量 蔗糖 2007 kg 2007 kg 2007 kg 2007 kg 2007 kg 豌豆蛋白水解物 922.5 kg 820.0 kg 717.5 kg 615.0 kg 512.5 kg 酪蛋白水解物 102.5 kg 205.0 kg 307.52 kg 410.0 kg 512.5 kg 改質木薯澱粉 989 kg 989 kg 989 kg 989 kg 989 kg 紅花油 644 kg 644 kg 644 kg 644 kg 644 kg 分館椰子油 568 kg 568 kg 568 kg 568 kg 568 kg 大豆油 479 kg 479 kg 479 kg 479 kg 479 kg 5% KOH(加工助劑) 187 kg 187 kg 187 kg 187 kg 187 kg 檸檬酸鈣 69.9 kg 69.9 kg 69.9 kg 69.9 kg 69.9 kg 磷酸氫鈣 48.1 kg 48.1 kg 48.1 kg 48.1 kg 48.1 kg 角叉菜膠 45.4 kg 45.4 kg 45.4 kg 45.4 kg 45.4 kg 磷酸氫二鉀 26.2 kg 26.2 kg 26.2 kg 26.2 kg 26.2 kg 抗壞血酸 24.2 kg 24.2 kg 24.2 kg 24.2 kg 24.2 kg 氣化鎂 18.6 kg 18.6 kg 18.6 kg 18.6 kg 18.6 kg 高山被孢黴油(ARA油) 17.9 kg 17.9 kg 17.9 kg 17.9 kg 17.9 kg 檸檬酸鉀 15.9 kg 15.9 kg 15.9 kg 15.9 kg 15.9 kg 氣化納 14.9 kg 14.9 kg 14.9 kg 14.9 kg 14.9 kg 氫氧化齊 14.0 kg 14.0 kg 14.0 kg 14.0 kg 14.0 kg L-胱胺酸二鹽酸鹽 12.1kg 12.1 kg 12.1 kg 12.1 kg 12.1 kg 氣化鉀 11.2 kg 11.2 kg 11.2 kg 11.2 kg 11.2 kg 水溶性維生素預混物 8.00 kg 8.00 kg 8.00 kg 8.00 kg 8.00 kg 藻類隱曱藻油(DHA油) 6.71 kg 6.71 kg 6.71 kg 6.71 kg 6.71 kg L-酪胺酸 5.08 kg 5.08 kg 5.08 kg 5.08 kg 5.08 kg L-色胺酸 4.67 kg 4.67 kg 4.67 kg 4.67 kg 4.67 kg 158197.doc -56- 201212828 氯化膽鹼 3.36 kg 3.36 kg 3.36 kg 3.36 kg 3.36 kg 油溶性維生素預混物 2.37 kg 2.37 kg 2.37 kg 2.37 kg 2.37 kg 硫酸亞鐵 2.32 kg 2.32 kg 2.32 kg 2.32 kg 2.32 kg L-肉驗 650 g 650 g 650 g 650 g 650 g 核黃素 87.9 g 87.9 g 87.9 g 87.9 g 87.9 g 維生素Α棕橺酸酯 17.6 g 17.6 g 17.6 g 17.6 g 17.6 g 破化卸 8.70 g 8.70 g 8.70 g 8.70 g 8.70 g 實例55-64 Ο 實例55-64說明本發明之營養乳液,其成分列於下表 中。為製備營養乳液,製備至少三種各別漿料(例如CHO-ΜΙΝ漿料、PIW漿料、PIF漿料),接著掺合在一起,熱處 理,均質化並標準化。將所得組合物無菌包裝至塑膠瓶中 或蒸餾滅菌。以下所述之包括豌豆蛋白水解物作為蛋白質 之唯一來源的實施例為低過敏性實施例。Fractionated coconut oil 568 kg 568 kg 568 kg 568 kg 568 kg soybean oil 479 kg 479 kg 479 kg 479 kg 479 kg 5% KOH (processing aid) 187 kg 187 kg 187 kg 187 kg 187 kg calcium citrate 69.9 kg 69.9 kg 69.9 kg 69.9 kg 69.9 kg Calcium hydrogen phosphate 48.1 kg 48.1 kg 48.1 kg 48.1 kg 48.1 kg Carrageenan 45.4 kg 45.4 kg 45.4 kg 45.4 kg 45.4 kg Dipotassium hydrogen phosphate 26.2 kg 26.2 kg 26.2 kg 26.2 kg 26.2 kg Ascorbic acid 24.2 kg 24.2 kg 24.2 kg 24.2 kg 24.2 kg Magnesium chloride 18.6 kg 18.6 kg 18.6 kg 18.6 kg 18.6 kg M. Alpina oil (ARA oil) 17.9 kg 17.9 kg 17.9 kg 17.9 kg 17.9 kg Potassium citrate 15.9 kg 15.9 kg 15.9 kg 15.9 kg 15.9 kg Gasification nano 14.9 kg 14.9 kg 14.9 kg 14.9 kg 14.9 kg Hydroxide #5 14.0 kg 14.0 kg 14.0 kg 14.0 kg 14.0 kg L-cystine dihydrochloride 12.1 kg 12.1 kg 12.1 kg 12.1 kg 12.1 kg Gasification potassium 11.2 kg 11.2 kg 11.2 kg 11.2 kg 11.2 kg Water-soluble vitamin premix 8.00 kg 8.00 kg 8.00 kg 8.00 kg 8.00 kg Algae Cryptophyta (C. 0?; 2 ring) oil (DHA oil) 6.71 Kg 6.71 kg 6.71 kg 6.71 kg 6.71 kg L- Amino acid 5.08 kg 5.08 kg 5.08 kg 5.08 kg 5.08 kg L-tryptophan 4.67 kg 4.67 kg 4.67 kg 4.67 kg 4.67 kg Gasification test 3.36 kg 3.36 kg 3.36 kg 3.36 kg 3.36 kg Oil-soluble vitamin premix 2.37 kg 2.37 Kg 2.37 kg 2.37 kg 2.37 kg ferrous sulfate 2.32 kg 2.32 kg 2.32 kg 2.32 kg 2.32 kg L-meat test 650 g 650 g 650 g 650 g 650 g riboflavin 87.9 g 87.9 g 87.9 g 87.9 g 87.9 g Vitamin A brown Phthalate 17.6 g 17.6 g 17.6 g 17.6 g 17.6 g Potassium depleted 8.70 g 8.70 g 8.70 g 8.70 g 8.70 g 158197.doc -55- 201212828 Example 50-54 Composition per 45359.24 kg Example 50 Example 51 Example 52 Example 53 Example 54 Ingredients in water sufficient in sufficient amount sufficient sucrose 2007 kg 2007 kg 2007 kg 2007 kg 2007 kg pea protein hydrolysate 922.5 kg 820.0 kg 717.5 kg 615.0 kg 512.5 kg casein hydrolysate 102.5 kg 205.0 kg 307.52 kg 410.0 kg 512.5 kg Modified tapioca starch 989 kg 989 kg 989 kg 989 kg 989 kg safflower oil 644 kg 644 kg 644 kg 644 kg 644 kg branch coconut oil 568 kg 568 kg 568 kg 568 kg 568 kg soybean oil 479 kg 479 kg 479 Kg 479 Kg 479 kg 5% KOH (processing aid) 187 kg 187 kg 187 kg 187 kg 187 kg calcium citrate 69.9 kg 69.9 kg 69.9 kg 69.9 kg 69.9 kg calcium hydrogen phosphate 48.1 kg 48.1 kg 48.1 kg 48.1 kg 48.1 kg carrageen Glue 45.4 kg 45.4 kg 45.4 kg 45.4 kg 45.4 kg Dipotassium hydrogen phosphate 26.2 kg 26.2 kg 26.2 kg 26.2 kg 26.2 kg Ascorbic acid 24.2 kg 24.2 kg 24.2 kg 24.2 kg 24.2 kg Magnesium oxide 18.6 kg 18.6 kg 18.6 kg 18.6 kg 18.6 kg Alpine Mortierella oil (ARA oil) 17.9 kg 17.9 kg 17.9 kg 17.9 kg 17.9 kg Potassium citrate 15.9 kg 15.9 kg 15.9 kg 15.9 kg 15.9 kg Gasification nano 14.9 kg 14.9 kg 14.9 kg 14.9 kg 14.9 kg Hydrogen peroxide 14.0 kg 14.0 Kg 14.0 kg 14.0 kg 14.0 kg L-cystine dihydrochloride 12.1 kg 12.1 kg 12.1 kg 12.1 kg 12.1 kg Gasification potassium 11.2 kg 11.2 kg 11.2 kg 11.2 kg 11.2 kg Water-soluble vitamin premix 8.00 kg 8.00 kg 8.00 Kg 8.00 kg 8.00 kg Algae cryptic algae oil (DHA oil) 6.71 kg 6.71 kg 6.71 kg 6.71 kg 6.71 kg L-tyramine 5.08 kg 5.08 kg 5.08 kg 5.08 kg 5.08 kg L-tryptophan 4.67 kg 4.67 kg 4.67 kg 4.67 kg 4.67 kg 158197.doc -56- 20 1212828 Choline chloride 3.36 kg 3.36 kg 3.36 kg 3.36 kg 3.36 kg Oil-soluble vitamin premix 2.37 kg 2.37 kg 2.37 kg 2.37 kg 2.37 kg Ferrous sulfate 2.32 kg 2.32 kg 2.32 kg 2.32 kg 2.32 kg L-meat test 650 g 650 g 650 g 650 g 650 g riboflavin 87.9 g 87.9 g 87.9 g 87.9 g 87.9 g Vitamin Α palm phthalate 17.6 g 17.6 g 17.6 g 17.6 g 17.6 g Decomposition 8.70 g 8.70 g 8.70 g 8.70 g 8.70 g Examples 55-64 实例 Examples 55-64 illustrate the nutritional emulsions of the present invention, the ingredients of which are listed in the table below. To prepare the nutritional emulsion, at least three separate slurries (e.g., CHO-ΜΙΝ slurries, PIW slurries, PIF slurries) are prepared, then blended together, heat treated, homogenized, and standardized. The resulting composition is aseptically packaged into a plastic bottle or sterilized by distillation. The examples described below including pea protein hydrolysate as the sole source of protein are hypoallergenic examples.
成分 每1000 kg 之量(kg) 實例55 實例56 實例57 實例58 實例59 水 足量 足量 足量 足量 足量 麥芽糊精 120.0 120.0 120.0 120.0 120.0 蔗糖 71.38 71.38 71.38 71.38 71.38 婉豆蛋白水解物 62.88 66.02 59.74 69.17 56.59 芥花油 27.5 27.5 27.5 27.5 27.5 玉米油 15.70 15.70 15.70 15.70 15.70 磷酸鎂 1.92 1.92 1.92 1.92 1.92 檸檬酸鉀 6.92 6.92 6.92 6.92 6.92 檸檬酸鈉 0.903 0.903 0.903 0.903 0.903 卵磷脂 1.50 1.50 1.50 1.50 1.50 158197.doc -57- 201212828 三磷酸鈉 1.06 1.06 1.06 1.06 1.06 磷酸氫二鉀 0.730 0.730 0.730 0.730 0.730 氯化鉀 1.04 1.04 1.04 1.04 1.04 抗壞血酸 0.235 0.235 0.235 0.235 0.235 角叉菜膠 0.150 0.150 0.150 0.150 0.150 氫氧化鉀 0.136 0.136 0.136 0.136 0.136 TM/UTM預混物 0.1684 0.1684 0.1684 0.1684 0.1684 結蘭膠 0.050 0.050 0.050 0.050 0.050 維生素A、D、E預混物 0.0758 0.0758 0.0758 0.0758 0.0758 水溶性維生素預混物 0.0728 0.0728 0.0728 0.0728 0.0728 峨化斜 0.00022 0.00022 0.00022 0.00022 0.00022 氯化鉻 0.000217 0.000217 0.000217 0.000217 0.000217 調味劑 3.3 3.3 3.3 3.3 3.3 成分 每1000 kg之量(kg) 實例60 實例61 實例62 實例63 實例64 水 足量 足量 足量 足量 足量 麥芽糊精 120.0 120.0 120.0 120.0 120.0 蔗糖 71.38 71.38 71.38 71.38 71.38 乳蛋白濃縮物 9.33 7.46 5.60 3.73 1.87 茶花油 27.5 27.5 27.5 27.5 27.5 婉豆蛋白水解物 31.50 39.61 41.82 55.34 50.93 赂蛋白納 13.25 10.67 8.00 5.34 2.67 大豆蛋白濃縮物 7.00 5.60 4.22 2.81 1.40 玉米油 15.70 15.70 15.70 15.70 15.70 乳清蛋白濃縮物 1.75 1.40 1.05 0.70 0.35 磷酸鎂 1.92 1.92 1.92 1.92 1.92 檸檬酸鉀 6.92 6.92 6.92 6.92 6.92 檸檬酸鈉 0.903 0.903 0.903 0.903 0.903 卵碌脂 1.50 1.50 1.50 1.50 1.50 158197.doc -58- 201212828 三磷酸鈉 1.06 1.06 1.06 1.06 1.06 磷酸氫二鉀 0.730 0.730 0.730 0.730 0.730 氯化鉀 1.04 1.04 1.04 1.04 1.04 抗壞血酸 0.235 0.235 0.235 0.235 0.235 角叉菜膠 0.150 0.150 0.150 0.150 0.150 氫氧化鉀 0.136 0.136 0.136 0.136 0.136 TM/UTM預混物 0.1684 0.1684 0.1684 0.1684 0.1684 結蘭膠 0.050 0.050 0.050 0.050 0.050 維生素A、D、E預混物 0.0758 0.0758 0.0758 0.0758 0.0758 水溶性維生素預混物 0.0728 0.0728 0.0728 0.0728 0.0728 峨化鉀 0.00022 0.00022 0.00022 0.00022 0.00022 氣化鉻 0.000217 0.000217 0.000217 0.000217 0.000217 調味劑 3.3 3.3 3.3 3.3 3.3Ingredient per 1000 kg (kg) Example 55 Example 56 Example 57 Example 58 Example 59 Water sufficient amount sufficient sufficient amount sufficient maltodextrin 120.0 120.0 120.0 120.0 120.0 sucrose 71.38 71.38 71.38 71.38 71.38 Kidney bean protein hydrolysate 62.88 66.02 59.74 69.17 56.59 Canola oil 27.5 27.5 27.5 27.5 27.5 Corn oil 15.70 15.70 15.70 15.70 15.70 Magnesium phosphate 1.92 1.92 1.92 1.92 1.92 Potassium citrate 6.92 6.92 6.92 6.92 6.92 Sodium citrate 0.903 0.903 0.903 0.903 0.903 Lecithin 1.50 1.50 1.50 1.50 1.50 158197.doc -57- 201212828 Sodium triphosphate 1.06 1.06 1.06 1.06 1.06 Dipotassium hydrogen phosphate 0.730 0.730 0.730 0.730 0.730 Potassium chloride 1.04 1.04 1.04 1.04 1.04 Ascorbic acid 0.235 0.235 0.235 0.235 0.235 Carrageenan 0.150 0.150 0.150 0.150 0.150 Potassium hydroxide 0.136 0.136 0.136 0.136 0.136 TM/UTM premix 0.1684 0.1684 0.1684 0.1684 0.1684 Lancture 0.050 0.050 0.050 0.050 0.050 Vitamin A, D, E premix 0.0758 0.0758 0.0758 0.0758 0.0758 Water-soluble vitamin premix 0.0728 0.0728 0.0728 0.0728 0.0728 峨Inclination 0.0002 2 0.00022 0.00022 0.00022 0.00022 Chromium chloride 0.000217 0.000217 0.000217 0.000217 0.000217 Flavoring agent 3.3 3.3 3.3 3.3 3.3 Ingredient per 1000 kg (kg) Example 60 Example 61 Example 62 Example 63 Example 64 Water sufficient amount sufficient to be sufficient Maltodextrin 120.0 120.0 120.0 120.0 120.0 Sucrose 71.38 71.38 71.38 71.38 71.38 Milk protein concentrate 9.33 7.46 5.60 3.73 1.87 Camellia oil 27.5 27.5 27.5 27.5 27.5 Cowpea protein hydrolysate 31.50 39.61 41.82 55.34 50.93 Brass protein nano 13.25 10.67 8.00 5.34 2.67 Soybean Protein Concentrate 7.00 5.60 4.22 2.81 1.40 Corn Oil 15.70 15.70 15.70 15.70 15.70 Whey Protein Concentrate 1.75 1.40 1.05 0.70 0.35 Magnesium Phosphate 1.92 1.92 1.92 1.92 1.92 Potassium Citrate 6.92 6.92 6.92 6.92 6.92 Sodium Citrate 0.903 0.903 0.903 0.903 0.903 Eggs Fat 1.50 1.50 1.50 1.50 1.50 158197.doc -58- 201212828 Sodium triphosphate 1.06 1.06 1.06 1.06 1.06 Dipotassium hydrogen phosphate 0.730 0.730 0.730 0.730 0.730 Potassium chloride 1.04 1.04 1.04 1.04 1.04 Ascorbic acid 0.235 0.235 0.235 0.235 0.235 Carrageenan 0.150 0.15 0 0.150 0.150 0.150 Potassium hydroxide 0.136 0.136 0.136 0.136 0.136 TM/UTM premix 0.1684 0.1684 0.1684 0.1684 0.1684 Lancture 0.050 0.050 0.050 0.050 0.050 Vitamin A, D, E premix 0.0758 0.0758 0.0758 0.0758 0.0758 Water-soluble vitamin premix Material 0.0728 0.0728 0.0728 0.0728 0.0728 Potassium sulphate 0.00022 0.00022 0.00022 0.00022 0.00022 Chromium oxide 0.000217 0.000217 0.000217 0.000217 0.000217 Flavoring agent 3.3 3.3 3.3 3.3 3.3
實例65-74 實例65-74說明本發明之實質上澄清營養液,其成分列 於下表中。為製備營養液,製備至少兩種各別漿料(例如 CHO-MIN漿料及PI W漿料),接著摻合在一起,熱處理, 均質化並標準化。將所得組合物無菌包裝至塑膠瓶中或蒸 餾滅菌。以下所述之包括婉豆蛋白水解物作為蛋白質之唯 一來源的實施例為低過敏性實施例。 成分 每1000 kg 之量(kg) 實例65 實例66 實例67 實例68 實例69 水 足量 足量 足量 足量 足量 蔗糖 109.0 109.0 0 54.5 54.5 異麥芽酮糖 0 0 109.0 54.5 54.5 婉豆蛋白水解物 42.90 45.05 40.76 47.19 38.61 158197.doc -59- 201212828 磷酸 2.0 2.0 2.0 2.0 2.0 檸檬酸 1.4 1.4 1.4 1.4 1.4 N&A蘋果調味劑 700 g 700 g 700 g 700 g 700 g 抗壞血酸 535 g 535 g 535 g 535 g 535 g 焦糖液體著色劑 400 g 400 g 400 g 400 g 400 g UTM/TM預混物 230 g 230 g 230 g 230 g 230 g 單水合硫酸鋅* 52.1 g 52.1 g 52.1 g 52.1 g 52.1 g 硫酸亞鐵* 40.8 g 40.8 g 40.8 g 40.8 g 40.8 g 檸檬酸* 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g 硫酸錳* 13.0 13.0 13.0 13.0 13.0 硫酸銅* 7.1 g 7.1 g 7-1 g 7.1 g 7.1 g 氣化鉻* 430 mg 430 mg 430 mg 430 mg 430 mg 钥酸鈉* 339 mg 339 mg 339 mg 339 mg 339 mg 硒酸鈉* 146 mg 146 mg 146 mg 146 mg 146 mg 水分散性ADEK預混物 178 g 178 g 178 g 178 g 178 g 二-α-生育酚乙酸酯** 45.3 g 45.3 g 45.3 g 45.3 g 45.3 g 維生素Α棕櫊酸酯** 4.2 g 4.2 g 4.2 g 4.2 g 4.2 g 葉綠醌** 127 mg 127 mg 127 mg 127 mg 127 mg 維生素D3** 23 mg 23 mg 23 mg 23 mg 23 mg WSV維生素預混物 37.9 g 37.9 g 37.9 g 37.9 g 37.9 g 菸鹼醯胺*** 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g d-泛酸約*** 9.2 g 9.2 g 9.2 g 9.2 g 9.2 g 鹽酸氣化硫胺素 2.4 g 2.4 g 2.4 g 2.4 g 2.4 g 鹽酸吡哆醇*** 23 g 2.3 g 2.3 g 2.3 g 2.3 g *UTM/TM預混物;**ADEK預混物;***WSV維生素預混物 158197.doc -60- 201212828Examples 65-74 Examples 65-74 illustrate the substantially clarified nutrient solutions of the present invention, the ingredients of which are listed in the table below. To prepare the nutrient solution, at least two separate slurries (e.g., CHO-MIN slurries and PI W slurries) are prepared, then blended together, heat treated, homogenized, and standardized. The resulting composition is aseptically packaged into a plastic bottle or sterilized by distillation. The following examples including the cowpea protein hydrolysate as the sole source of protein are hypoallergenic examples. Ingredient per 1000 kg (kg) Example 65 Example 66 Example 67 Example 68 Example 69 Water sufficient amount sufficient sufficient amount sufficient sucrose 109.0 109.0 0 54.5 54.5 isomaltulose 0 0 109.0 54.5 54.5 42.90 45.05 40.76 47.19 38.61 158197.doc -59- 201212828 Phosphoric acid 2.0 2.0 2.0 2.0 2.0 Citric acid 1.4 1.4 1.4 1.4 1.4 N&A apple flavoring 700 g 700 g 700 g 700 g 700 g Ascorbic acid 535 g 535 g 535 g 535 g 535 g caramel liquid colorant 400 g 400 g 400 g 400 g 400 g UTM/TM premix 230 g 230 g 230 g 230 g 230 g zinc sulfate monohydrate* 52.1 g 52.1 g 52.1 g 52.1 g 52.1 g sulfuric acid Ferrous * 40.8 g 40.8 g 40.8 g 40.8 g 40.8 g citric acid * 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g manganese sulfate * 13.0 13.0 13.0 13.0 13.0 copper sulfate * 7.1 g 7.1 g 7-1 g 7.1 g 7.1 g gas Chromium* 430 mg 430 mg 430 mg 430 mg 430 mg sodium sulphate* 339 mg 339 mg 339 mg 339 mg 339 mg sodium selenate* 146 mg 146 mg 146 mg 146 mg 146 mg water-dispersible ADEK premix 178 g 178 g 178 g 178 g 178 g di-α-tocopheryl acetate** 45.3 g 45.3 g 45.3 g 45.3 g 45.3 g Vitamin Α palmitate** 4.2 g 4.2 g 4.2 g 4.2 g 4.2 g chlorophyll ** 127 mg 127 mg 127 mg 127 mg 127 mg vitamin D3** 23 mg 23 mg 23 mg 23 mg 23 Mg WSV vitamin premix 37.9 g 37.9 g 37.9 g 37.9 g 37.9 g Nicotine guanamine *** 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g d-pantothenic acid *** 9.2 g 9.2 g 9.2 g 9.2 g 9.2 g Hydrochloric acid thiamine 2.4 g 2.4 g 2.4 g 2.4 g 2.4 g pyridoxine hydrochloride *** 23 g 2.3 g 2.3 g 2.3 g 2.3 g *UTM/TM premix; **ADEK premix; *WSV vitamin premix 158197.doc -60- 201212828
成分 每1000 kg之量(kg) 實例70 實例71 實例72 實例73 實例74 水 足量 足量 足量 足量 足量 蔗糖 109.0 109.0 0 54.5 54.5 異麥芽酮糖 0 0 109.0 54.5 54.5 疏豆蛋白水解物 21.45 27.03 28.53 37.75 34.75 乳清蛋白分離物 18.59 12.87 11.15 6.24 3.44 水解酪蛋白 2.86 4.29 1.72 2.14 0.75 磷酸 2.0 2.0 2.0 2.0 2.0 檸檬酸 1.4 1.4 1.4 1.4 1.4 N&A蘋果調味劑 700 g 700 g 700 g 700 g 700 g 抗壞血酸 535 g 535 g 535 g 535 g 535 g 焦糖液體著色劑 400 g 400 g 400 g 400 g 400 g UTM/TM預混物 230 g 230 g 230 g 230 g 230 g 單水合硫酸辞* 52.1 g 52.1 g 52.1 g 52.1 g 52.1 g 硫酸亞鐵* 40.8 g 40.8 g 40.8 g 40.8 g 40.8 g 檸檬酸* 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g 硫酸錳* 13.0 13.0 13.0 13.0 13.0 硫酸銅* 7.1 g 7.1 g 7.1 g 7.1 g 7.1 g 氣化鉻* 430 mg 430 mg 430 mg 430 mg 430 mg 鉬酸鈉* 339 mg 339 mg 339 mg 339 mg 339 mg 硒酸鈉* 146 mg 146 mg 146 mg 146 mg 146 mg 水分散性ADEK預混物 178 g 178 g 178 g 178 g 178 g 二-α-生育酚乙酸酯** 45.3 g 45.3 g 45.3 g 45.3 g 45.3 g 維生素Α棕櫊酸酯** 4.2 g 4.2 g 4.2 g 4.2 g 4.2 g 葉綠醌** 127 mg 127 mg 127 mg 127 mg 127 mg 維生素D3** 23 mg 23 mg 23 mg 23 mg 23 mg WSV維生素預混物 37.9 g 37.9 g 37.9 g 37.9 g 37.9 g 158197.doc -61 · 201212828 菸鹼醯胺*** 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g d-泛酸妈*** 9.2 g 9.2 g 9.2 g 9.2 g 9.2 g 鹽酸氣化硫胺素*** 2.4 g 2.4 g 2.4 g 2.4 g 2.4 g 鹽酸吡哆醇*** 2.3 g 2.3 g 2.3 g 2.3 g 2.3 g 核黃素*** 1.8 g 18 g 1.8 g 1.8 g 1.8 g 葉酸*** 350 mg 350 mg 350 mg 350 mg 350 mg 生物素*** 277 mg 277 mg 277 mg 277 mg 277 mg 氰鈷胺*** 6.3 mg 6.3 mg 6.3 mg 6.3 mg 6.3 mg 葉酸 1.3 g 1.3 g 1.3 g 1.3 g 1-3 g 峨化鉀 204 mg 204 mg 204 mg 204 mg 204 mg β-丙胺酸 0 0 0 1.0 1.0 *UTM/TM預混物;**ADEK預混物;***WSV維生素預混物 實例75-84 實例75-84說明本發明之濃縮液體人乳強化劑,其成分 列於下表中。為製備濃縮液體人乳強化劑,製備至少三種 各別漿料(例如CHO-MIN漿料及PIW漿料),接著摻合在一 起。以下所述之包括豌豆蛋白水解物作為蛋白質之唯一來 源的實施例為低過敏性實施例。 成分 每1000 lbs之量 實例75 實例76 實例77 實例78 實例79 水 足量 足量 足量 足量 足量 婉豆蛋白水解物 104.61b 99.4 lb 109.8 lb 94.1 lb 115.1 lb 麥芽糊精 93.0 lb 93.0 lb 93.0 lb 93.01b 93.0 lb MCT油 21.61b 21.61b 21.6 lb 21.6 lb 21.61b 超微米尺寸化磷酸三鈣 17.8 lb 17.81b 17.81b 17.81b 17.8 lb 大豆油 12.9 lb 12.91b 12.9 lb 12.9 lb 12.9 lb 158197.doc •62· 201212828Ingredients per 1000 kg (kg) Example 70 Example 71 Example 72 Example 73 Example 74 Water sufficient amount sufficient sufficient amount sufficient sucrose 109.0 109.0 0 54.5 54.5 isomaltulose 0 0 109.0 54.5 54.5 21.45 27.03 28.53 37.75 34.75 Whey Protein Isolate 18.59 12.87 11.15 6.24 3.44 Hydrolyzed Casein 2.86 4.29 1.72 2.14 0.75 Phosphoric Acid 2.0 2.0 2.0 2.0 2.0 Citric Acid 1.4 1.4 1.4 1.4 1.4 N&A Apple Flavor 700 g 700 g 700 g 700 g 700 g ascorbic acid 535 g 535 g 535 g 535 g 535 g caramel liquid colorant 400 g 400 g 400 g 400 g 400 g 400 g UTM/TM premix 230 g 230 g 230 g 230 g 230 g monohydrate sulfuric acid* 52.1 g 52.1 g 52.1 g 52.1 g 52.1 g ferrous sulfate* 40.8 g 40.8 g 40.8 g 40.8 g 40.8 g citric acid* 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g Manganese sulfate* 13.0 13.0 13.0 13.0 13.0 Copper sulphate* 7.1 g 7.1 g 7.1 g 7.1 g 7.1 g vaporized chromium* 430 mg 430 mg 430 mg 430 mg 430 mg sodium molybdate* 339 mg 339 mg 339 mg 339 mg 339 mg sodium selenate* 146 mg 146 mg 146 mg 146 mg 146 mg Water-dispersible ADEK premix 178 g 178 g 1 78 g 178 g 178 g Di-α-tocopherol acetate** 45.3 g 45.3 g 45.3 g 45.3 g 45.3 g Vitamin Α palmitate** 4.2 g 4.2 g 4.2 g 4.2 g 4.2 g Leaf green 醌127 mg 127 mg 127 mg 127 mg 127 mg vitamin D3** 23 mg 23 mg 23 mg 23 mg 23 mg WSV vitamin premix 37.9 g 37.9 g 37.9 g 37.9 g 37.9 g 158197.doc -61 · 201212828 Nicotinamide *** 14.2 g 14.2 g 14.2 g 14.2 g 14.2 g d-pantothenic acid *** 9.2 g 9.2 g 9.2 g 9.2 g 9.2 g Hydrochloric acid thiamine *** 2.4 g 2.4 g 2.4 g 2.4 g 2.4 g Hydrochloric acid Pyridoxine *** 2.3 g 2.3 g 2.3 g 2.3 g 2.3 g riboflavin *** 1.8 g 18 g 1.8 g 1.8 g 1.8 g folic acid *** 350 mg 350 mg 350 mg 350 mg 350 mg biotin** * 277 mg 277 mg 277 mg 277 mg 277 mg cyanocobalamin *** 6.3 mg 6.3 mg 6.3 mg 6.3 mg 6.3 mg folic acid 1.3 g 1.3 g 1.3 g 1.3 g 1-3 g potassium telluride 204 mg 204 mg 204 mg 204 Mg 204 mg β-alanine 0 0 0 1.0 1.0 *UTM/TM premix; **ADEK premix; ***WSV vitamin premix Example 75-84 Examples 75-84 illustrate the concentrated liquid person of the present invention Milk fortifiers, the ingredients of which are listed in the table below. To prepare a concentrated liquid human milk fortifier, at least three separate slurries (e.g., CHO-MIN slurries and PIW slurries) are prepared and then blended together. The examples described below which include pea protein hydrolysate as the sole source of protein are hypoallergenic examples. Ingredients per 1000 lbs Example 75 Example 76 Example 77 Example 78 Example 79 Water sufficient amount sufficient sufficient sufficient amount of cowpea protein hydrolysate 104.61b 99.4 lb 109.8 lb 94.1 lb 115.1 lb maltodextrin 93.0 lb 93.0 lb 93.0 lb 93.01b 93.0 lb MCT oil 21.61b 21.61b 21.6 lb 21.6 lb 21.61b Ultra-micronized tricalcium phosphate 17.8 lb 17.81b 17.81b 17.81b 17.8 lb Soybean oil 12.9 lb 12.91b 12.9 lb 12.9 lb 12.9 lb 158197.doc •62· 201212828
OSA改質玉米澱粉 12.0 lb 12.0 lb 12.0 lb 12.0 lb 12.0 lb 椰子油 7.91b 7.9 lb 7.91b 7.91b 7.91b 擰樣酸鉀 7.01b 7.0 lb 7.01b 7.01b 7.01b 抗壞血酸 5.41b 5.41b 5.41b 5.41b 5.4 lb 氯化鎂 3.91b 3.91b 3.91b 3.91b 3.9 lb ARA 3.4 lb 3.41b 3.41b 3.41b 3.41b L-白胺酸 2.81b 2.81b 2.81b 2.8 lb 2.81b DHA 2.61b 2.61b 2.61b 2.61b 2.61b 氣化鉀 1.61b 1.61b 1.61b 1.61b 1.61b L-酪胺酸 1.4 lb 1.41b 1.41b 1.41b 1.4 lb 蒸館單酸甘油醋 1.01b 1.01b 1.01b 1.01b 1.01b 核苷酸/膽鹼預混物 399.0 g 399.0 g 399.0 g 399.0 g 399.0 g M-肌醇 296.0 g 296.0 g 296.0 g 296.0 g 296.0 g 氣化鈉 246.3 g 246.3 g 246.3 g 246.3 g 246.3 g L-肉鹼 147.0 g 147.0 g 147.0 g 147.0 g 147.0 g 硫酸辞 127.0 g 127.0 g 127.0 g 127.0 g 127.0 g 於驗酿胺 124.0 g 124.0 g 124.0 g 124.0 g 124.0 g dl-α-生育酚乙酸酯 114.0 g 114.0 g 114.0 g 114.0 g 114.0 g 結蘭膠 90.7 g 90.7 g 90.7 g 90.7 g 90.7 g 硫酸亞鐵 56.0 g 56.0 g 56.0 g 56.0 g 56.0 g 氯化膽驗 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g 泛酸鈣 39.3 g 39.3 g 39.3 g 39.3 g 39.3 g 維生素A棕櫚酸酯 24.0 g 24.0 g 24.0 g 24.0 g 24.0 g 核黃素 9.4 g 9.4 g 9.4 g 9.4 g 9.4 g 維生素D3 8.6 g 8.6 g 8.6 g 8.6 g 8.6 g 硫酸鋼 8.2 g 8.2 g 8.2 g 8.2 g 8.2 g 鹽酸D比吟醇 6.3 g 6.3 g 6.3 g 6.3 g 6.3 g 鹽酸硫胺素 6.1 g 6.1 g 6.1 g 6.1 g 6.1 g 葉黃素 2.5 g 2.5 g 2.5 g 2.5 g 2.5 g 158197.doc ·63· 201212828 β胡蘿蔔素 1-7 g 1.7 g 1.7 g 1.7 g 1.7 g 葉酸 0.88 g 0.88 g 0.88 g 0.88 g 0.88 g 生物素 0.74 g 0.74 g 0.74 g 0.74 g 0.74 g 硫酸錳 06.8 g 06.8 g 06.8 g 06.8 g 06.8 g 葉綠醌 0.31 g 0.31 g 0.31 g 0.31 g 0.31 g 砸酸納 0.06 g 0.06 g 0.06 g 0.06 g 0.06 g 氰鈷胺 0.02 g 0.02 g 0.02 g 0.02 g 0.02 g 成分 :1000 lbs之 默 實例80 實例81 實例82 實例83 實例84 水 足量 足量 足量 足量 足量 豌豆蛋白水解物 52.3 lb 62.76 lb 73.22 lb. 83.68 lb 94.14 lb 鈣水解物 52.3 lb 41.841b 31.381b 20.92 lb 10.46 lb 麥芽糊精 93.0 lb 93.0 lb 93.0 lb 93.0 lb 93.0 lb MCT油 21.6 lb 21.61b 21.61b 21.6 lb 21.61b 超微米尺寸化鱗酸三妈 17.81b 17.8 lb 17.81b 17.81b 17.8 lb 大豆油 12.9 lb 12.9 lb 12.9 lb 12.91b 12.9 lb OSA改質玉米澱粉 12.0 lb 12.0 lb 12.0 lb 12.0 lb 12.0 lb 椰子油 7.91b 7.91b 7.91b 7.91b 7.91b 檸檬酸卸 7.01b 7.01b 7.01b 7.01b 7.01b 抗壞血酸 5.41b 5.41b 5.41b 5.41b 5.4 lb 氯化鎂 3.91b 3.9 lb 3.91b 3.91b 3.91b ARA 3.4 lb 3.41b 3.4 lb 3.4 lb 3.41b L-白胺酸 2.81b 2.81b 2.81b 2.81b 2.81b DHA 2.61b 2.61b 2.61b 2.61b 2.61b 氣化鉀 1.6 lb 1.61b 1.61b 1.61b 1.61b L-酪胺酸 1.4 lb 1.4 lb 1.41b 1.4 lb 1.4 lb 蒸館單酸甘油醋 1.0 lb 1.0 lb 1.01b 1.01b 1.01b 核苷酸/膽鹼預混物 399.0 g 399.0 g 399.0 g 399.0 g 399.0 g M-肌醇 296.0 g 296.0 g 296.0 g 296.0 g 296.0 g 158197.doc 201212828 氣化鈉 246.3 g 246.3 g 246.3 g 246.3 g 246.3 g L-肉驗 147.0 g 147.0 g 147.0 g 147.0 g 147.0 g 硫酸辞 127.0 g 127.0 g 127.0 g 127.0 g 127.0 g 菸鹼醯胺 124.0 g 124.0 g 124.0 g 124.0 g 124.0 g dl-α-生育酚乙酸酯 114.0 g 114.0 g 114.0 g 114.0 g 114.0 g 結蘭膠 90.7 g 90.7 g 90.7 g 90.7 g 90.7 g 硫酸亞鐵 56.0 g 56.0 g 56.0 g 56.0 g 56.0 g 氣化膽鹼 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g 泛酸鈣 39.3 g 39.3 g 39.3 g 39.3 g 39.3 g 維生素A棕櫚酸酯 24.0 g 24.0 g 24.0 g 24.0 g 24.0 g 核黃素 9.4 g 9.4 g 9.4 g 9.4 g 9.4 g 維生素D3 8.6 g 8.6 g 8.6 g 8.6 g 8.6 g 硫酸銅 8.2 g 8.2 g 8.2 g 8.2 g 8.2 g 鹽酸°比哆醇 6.3 g 6.3 g 6.3 g 6.3 g 6.3 g 鹽酸硫胺素 6.1 g 6.1 g 6.1 g 6.1 g 6.1 g 葉黃素 2.5 g 2.5 g 2.5 g 2.5 g 2.5 g β胡蘿蔔素 1-7 g 1.7 g 1.7 g 1.7 g 1-7 g 葉酸 0.88 g 0.88 g 0.88 g 0.88 g 0.88 g 生物素 0.74 g 0.74 g 0.74 g 0.74 g 0.74 g 硫酸錳 06.8 g 06.8 g 06.8 g 06.8 g 06.8 g 葉綠醌 0.31 g 0.31 g 0.31 g 0.31 g 0.31 g 石西酸納 0.06 g 0.06 g 0.06 g 0.06 g 0.06 g 氰鈷胺 0.02 g 0.02 g 0.02 g 0.02 g 0.02 g 實例85-94 以下實例說明人乳強化劑粉,其添加至可根據本發明之 方法投與至嬰兒之人乳中。製造8,172 0人乳強化劑粉之 成分列於下表中。 158197.doc •65· 201212828 成分 用於調配8,172 kg強化劑之量 實例85 實例86 實例87 實例88 實例89 成分水 16,205 L 16,205 L 16,205 L 16,205 L 16,205 L 玉米糖漿固體 1603 kg 1603 kg 1603 kg 1603 kg 1603 kg 氯化鎂 96.2 kg 96.2 kg 96.2 kg 96.2 kg 96.2 kg 擰檬酸鉀 223.8 kg 223.8 kg 223.8 kg 223.8 kg 223.8 kg 擰檬酸鈉 6.6 kg 6.6 kg 6.6 kg 6.6 kg 6.6 kg 氯化鈉 15.4 kg 15.4 kg 15.4 kg 15.4 kg 15.4 kg MTC油 801kg 801 kg 801 kg 801 kg 801 kg 卵磷脂 16.6 kg 16.6 kg 16.6 kg 16.6 kg 16.6 kg 維生素A 2.36 kg 2.36 kg 2.36 kg 2.36 kg 2.36 kg 維生素D 359.3 g 359.3 g 359.3 g 359.3 g 359.3 g 維生素K 27.5 g 27.5 g 27.5 g 27.5 g 27.5 g 生育酚乙酸酯 15.8 kg 15.8 kg 15.8 kg 15.8 kg 15.8 kg 碳酸鈣 33.1 kg 33.1 kg 33.1 kg 33.1kg 33.1 kg 磷酸三鈣 646 kg 646 kg 646 kg 646 kg 646 kg 婉豆蛋白水解物 2696.5 kg 2561.7 kg 2831.3 kg 2426.9 kg 2966.2 kg 檸檬酸鉀 257.2 g 257.2 g 257.2 g 257.2 g 257.2 g 硫酸亞鐵 3.7 kg 3.7 kg 3.7 kg 3.7 kg 3.7 kg 硫酸鋅 11.1kg 11.1kg 11.1kg 11.1kg 11.1kg 硫酸銅 1.84 kg 1.84 kg 1.84 kg 1.84 kg 1.84 kg 硫酸猛 0.320 kg 0.320 kg 0.320 kg 0.320 kg 0.320 kg 石西酸納 0.001 kg 0.001 kg 0.001 kg 0.001 kg 0.001 kg 菸鹼醯胺 0.98 kg 0.98 kg 0.98 kg 0.98 kg 0.98 kg 核黃素 1.14 kg 1.14 kg 1.14kg 1.14 kg 1.14 kg 泛酸鈣 4.08 kg 4.08 kg 4.08 kg 4.08 kg 4.08 kg 鹽酸。比哆醇 0.655 kg 0.655 kg 0.655 kg 0.655 kg 0.655 kg m-肌醇 9.55 kg 9.55 kg 9.55 kg 9.55 kg 9.55 kg 158197.doc -66- 201212828OSA Modified Corn Starch 12.0 lb 12.0 lb 12.0 lb 12.0 lb 12.0 lb Coconut Oil 7.91b 7.9 lb 7.91b 7.91b 7.91b Sodium Potassium 7.01b 7.0 lb 7.01b 7.01b 7.01b Ascorbic Acid 5.41b 5.41b 5.41b 5.41b 5.4 lb Magnesium chloride 3.91b 3.91b 3.91b 3.91b 3.9 lb ARA 3.4 lb 3.41b 3.41b 3.41b 3.41b L-leucine 2.81b 2.81b 2.81b 2.8 lb 2.81b DHA 2.61b 2.61b 2.61b 2.61b 2.61b Gasification Potassium 1.61b 1.61b 1.61b 1.61b 1.61b L-tyramine 1.4 lb 1.41b 1.41b 1.41b 1.4 lb Steaming Monoglyceride 1.01b 1.01b 1.01b 1.01b 1.01b Nucleotide/choline Premix 399.0 g 399.0 g 399.0 g 399.0 g 399.0 g M-inositol 296.0 g 296.0 g 296.0 g 296.0 g 296.0 g sodium gas 246.3 g 246.3 g 246.3 g 246.3 g 246.3 g L-carnitine 147.0 g 147.0 g 147.0 g 147.0 g 147.0 g Sulfuric acid 127.0 g 127.0 g 127.0 g 127.0 g 127.0 g Fermentation amine 124.0 g 124.0 g 124.0 g 124.0 g 124.0 g dl-α-tocopheryl acetate 114.0 g 114.0 g 114.0 g 114.0 g 114.0 g Lacquer 90.7 g 90.7 g 90.7 g 90.7 g 90.7 g ferrous sulfate 56.0 g 56.0 g 56.0 g 56.0 g 56.0 g chlorination test 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g calcium pantothenate 39.3 g 39.3 g 39.3 g 39.3 g 39.3 g vitamin A palmitate 24.0 g 24.0 g 24.0 g 24.0 g 24.0 g riboflavin 9.4 g 9.4 g 9.4 g 9.4 g 9.4 g vitamin D3 8.6 g 8.6 g 8.6 g 8.6 g 8.6 g sulfuric acid steel 8.2 g 8.2 g 8.2 g 8.2 g 8.2 g hydrochloric acid D sterol 6.3 g 6.3 g 6.3 g 6.3 g 6.3 g thiamine hydrochloride 6.1 g 6.1 g 6.1 g 6.1 g 6.1 g Lutein 2.5 g 2.5 g 2.5 g 2.5 g 2.5 g 158197.doc ·63· 201212828 beta carotene 1-7 g 1.7 g 1.7 g 1.7 g 1.7 g folic acid 0.88 g 0.88 g 0.88 g 0.88 g 0.88 g biotin 0.74 g 0.74 g 0.74 g 0.74 g 0.74 g Manganese sulfate 06.8 g 06.8 g 06.8 g 06.8 g 06.8 g Leaf green 醌 0.31 g 0.31 g 0.31 g 0.31 g 0.31 g sodium citrate 0.06 g 0.06 g 0.06 g 0.06 g 0.06 g Cyanocobalamin 0.02 g 0.02 g 0.02 g 0.02 g 0.02 g Composition: 1000 lbs of exemplified Example 80 Example 81 Example 82 Example 83 Example 84 Water sufficient amount sufficient sufficient amount sufficient pea protein hydrolysate 52.3 lb 62.76 lb 73.22 lb. 83.68 lb 94.14 Lb Calcium Hydrolysate 52.3 lb 41.841b 31.381b 20.92 lb 10.46 lb Maltodextrin 93.0 lb 93.0 Lb 93.0 lb 93.0 lb 93.0 lb MCT Oil 21.6 lb 21.61b 21.61b 21.6 lb 21.61b Ultra Micron Size Syrup Sanma 17.81b 17.8 lb 17.81b 17.81b 17.8 lb Soybean Oil 12.9 lb 12.9 lb 12.9 lb 12.91b 12.9 lb OSA Modified Corn Starch 12.0 lb 12.0 lb 12.0 lb 12.0 lb 12.0 lb Coconut Oil 7.91b 7.91b 7.91b 7.91b 7.91b Citric Acid Unloading 7.01b 7.01b 7.01b 7.01b 7.01b Ascorbic Acid 5.41b 5.41b 5.41b 5.41b 5.4 lb Magnesium chloride 3.91b 3.9 lb 3.91b 3.91b 3.91b ARA 3.4 lb 3.41b 3.4 lb 3.4 lb 3.41b L-leucine 2.81b 2.81b 2.81b 2.81b 2.81b DHA 2.61b 2.61b 2.61b 2.61b 2.61b Gasification Potassium 1.6 lb 1.61b 1.61b 1.61b 1.61b L-tyramine 1.4 lb 1.4 lb 1.41b 1.4 lb 1.4 lb Steaming Monoglyceride 1.0 lb 1.0 lb 1.01b 1.01b 1.01b Nucleotide/choline premix 399.0 g 399.0 g 399.0 g 399.0 g 399.0 g M-inositol 296.0 g 296.0 g 296.0 g 296.0 g 296.0 g 158197.doc 201212828 sodium gas 246.3 g 246.3 g 246.3 g 246.3 g 246.3 g L-meat 147.0 g 147.0 g 147.0 g 147.0 g 147.0 g Sulfuric acid 127.0 g 127.0 g 127.0 g 127.0 g 127.0 g Nicotinamide 124.0 g 124 .0 g 124.0 g 124.0 g 124.0 g dl-α-tocopherol acetate 114.0 g 114.0 g 114.0 g 114.0 g 114.0 g lanolin 90.7 g 90.7 g 90.7 g 90.7 g 90.7 g ferrous sulfate 56.0 g 56.0 g 56.0 g 56.0 g 56.0 g gasified choline 48.0 g 48.0 g 48.0 g 48.0 g 48.0 g calcium pantothenate 39.3 g 39.3 g 39.3 g 39.3 g 39.3 g vitamin A palmitate 24.0 g 24.0 g 24.0 g 24.0 g 24.0 g riboflavin 9.4 g 9.4 g 9.4 g 9.4 g 9.4 g vitamin D3 8.6 g 8.6 g 8.6 g 8.6 g 8.6 g copper sulfate 8.2 g 8.2 g 8.2 g 8.2 g 8.2 g hydrochloric acid sterol 6.3 g 6.3 g 6.3 g 6.3 g 6.3 g thiamine hydrochloride 6.1 g 6.1 g 6.1 g 6.1 g 6.1 g lutein 2.5 g 2.5 g 2.5 g 2.5 g 2.5 g beta carotene 1-7 g 1.7 g 1.7 g 1.7 g 1-7 g folic acid 0.88 g 0.88 g 0.88 g 0.88 g 0.88 g Biotin 0.74 g 0.74 g 0.74 g 0.74 g 0.74 g Manganese sulfate 06.8 g 06.8 g 06.8 g 06.8 g 06.8 g Leaf green 醌 0.31 g 0.31 g 0.31 g 0.31 g 0.31 g Salicillin 0.06 g 0.06 g 0.06 g 0.06 g 0.06 g cyanocobalamin 0.02 g 0.02 g 0.02 g 0.02 g 0.02 g Example 85-94 The following examples illustrate human milk fortifier powder, which can be added to The method of the invention is administered to human milk in infants. The ingredients for making 8,172 0 human milk fortifier powder are listed in the table below. 158197.doc •65· 201212828 Ingredients used to formulate 8,172 kg of fortifier Example 85 Example 86 Example 87 Example 88 Example 89 Ingredient water 16,205 L 16,205 L 16,205 L 16,205 L 16,205 L Corn syrup solid 1603 kg 1603 kg 1603 kg 1603 kg 1603 kg Magnesium chloride 96.2 kg 96.2 kg 96.2 kg 96.2 kg 96.2 kg Potassium citrate 223.8 kg 223.8 kg 223.8 kg 223.8 kg 223.8 kg Sodium citrate 6.6 kg 6.6 kg 6.6 kg 6.6 kg 6.6 kg Sodium chloride 15.4 kg 15.4 kg 15.4 kg 15.4 kg 15.4 kg MTC oil 801kg 801 kg 801 kg 801 kg 801 kg Lecithin 16.6 kg 16.6 kg 16.6 kg 16.6 kg 16.6 kg Vitamin A 2.36 kg 2.36 kg 2.36 kg 2.36 kg 2.36 kg Vitamin D 359.3 g 359.3 g 359.3 g 359.3 g 359.3 g vitamin K 27.5 g 27.5 g 27.5 g 27.5 g 27.5 g tocopherol acetate 15.8 kg 15.8 kg 15.8 kg 15.8 kg 15.8 kg calcium carbonate 33.1 kg 33.1 kg 33.1 kg 33.1 kg 33.1 kg tricalcium phosphate 646 kg 646 kg 646 kg 646 Kg 646 kg cowpea protein hydrolysate 2696.5 kg 2561.7 kg 2831.3 kg 2426.9 kg 2966.2 kg potassium citrate 257.2 g 257.2 g 257.2 g 257.2 g 257.2 g ferrous sulfate 3.7 kg 3.7 Kg 3.7 kg 3.7 kg 3.7 kg zinc sulfate 11.1kg 11.1kg 11.1kg 11.1kg 11.1kg copper sulfate 1.84 kg 1.84 kg 1.84 kg 1.84 kg 1.84 kg sulfuric acid 0.320 kg 0.320 kg 0.320 kg 0.320 kg 0.320 kg sodium sulphate 0.001 kg 0.001 Kg 0.001 kg 0.001 kg 0.001 kg Nicotine decylamine 0.98 kg 0.98 kg 0.98 kg 0.98 kg 0.98 kg Riboflavin 1.14 kg 1.14 kg 1.14 kg 1.14 kg 1.14 kg Calcium pantothenate 4.08 kg 4.08 kg 4.08 kg 4.08 kg 4.08 kg Hydrochloric acid. Compared to sterols 0.655 kg 0.655 kg 0.655 kg 0.655 kg 0.655 kg m-inositol 9.55 kg 9.55 kg 9.55 kg 9.55 kg 9.55 kg 158197.doc -66- 201212828
生物素 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg 葉酸 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 氰鈷胺 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 抗壞血酸 153.5 kg 153.5 kg 153.5 kg 153.5 kg 153.5 kg 成分 用於調配8,172 kg強化劑之量 實例90 實例91 實例92 實例93 實例94 成分水 16,205 L 16,205 L 16,205 L 16,205 L 16,205 L 玉米糖漿固體 1603 kg 1603 kg 1603 kg 1603 kg 1603 kg 氯化鎂 96.2 kg 96.2 kg 96.2 kg 96.2 kg 96.2 kg 擰檬酸鉀 223.8 kg 223.8 kg 223.8 kg 223.8 kg 223.8 kg 檸檬酸鈉 6.6 kg 6.6 kg 6.6 kg 6.6 kg 6.6 kg 氯化鈉 15.4 kg 15.4 kg 15.4 kg 15.4 kg 15.4 kg MTC油 801 kg 801 kg 801 kg 801 kg 801 kg 卵鱗脂 16.6 kg 16.6 kg 16.6 kg 16.6 kg 16.6 kg 維生素A 2.36 kg 2.36 kg 2.36 kg 2.36 kg 2.36 kg 維生素D 359.3 g 359.3 g 359.3 g 359.3 g 359.3 g 維生素K 27.5 g 27.5 g 27.5 g 27.5 g 27.5 g 生育酚乙酸酯 15.8 kg 15.8 kg 15.8 kg 15.8 kg 15.8 kg 碳酸鈣 33.1 kg 33.1 kg 33.1kg 33.1 kg 33.1kg 磷酸三鈣 646 kg 646 kg 646 kg 646 kg 646 kg 婉豆蛋白水解物 1348.3 kg 1537.0 kg 1981.9 kg 1941.5 kg 2669.58 kg 大豆蛋白濃縮物 753.0 kg 602.4 kg 451.8 kg 301.2 kg 150.6 kg 脫脂乳粉 1653.5 kg 1322.8 kg 992.1 kg 661.4 kg 3.31 kg 檸檬酸鉀 257.2 g 257.2 g 257.2 g 257.2 g 257.2 g 硫酸亞鐵 3.7 kg 3.7 kg 3.7 kg 3.7 kg 3.7 kg 硫酸辞 11.1kg 11.1kg 11.1kg 11.1kg 11.1kg 硫酸銅 1.84 kg 1.84 kg 1.84 kg 1.84 kg 1.84 kg 硫酸猛 0.320 kg 0.320 kg 0.320 kg 0.320 kg 0.320 kg 碰酸納 0.001 kg 0.001 kg 0.001 kg 0.001 kg 0.001 kg 158197.doc •67- 201212828 菸鹼醯胺 0.98 kg 0.98 kg 0.98 kg 0.98 kg 0.98 kg 核黃素 1.14 kg 1.14 kg 1.14 kg 1.14 kg 1.14 kg 泛酸鈣 4.08 kg 4.08 kg 4.08 kg 4.08 kg 4.08 kg 鹽酸°比哆醇 0.655 kg 0.655 kg 0.655 kg 0.655 kg 0.655 kg m-肌醇 9.55 kg 9.55 kg 9.55 kg 9.55 kg 9.55 kg 生物素 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg 葉酸 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 氰鈷胺 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 抗壞血酸 153.5 kg 153.5 kg 153.5 kg 153.5 kg 153.5 kg 實例95-104 在此等實例中,根據本發明製備膠凝人乳強化劑。膠凝 人乳強化劑之成分展示於下表中。 藉由將成分溶解及組合於進行適當熱處理以達成長期存 放穩定性之均質水性混合物中來製備膠凝人乳強化劑。 為開始製造過程,將供應巨量營養素(碳水化合物、蛋 白質、脂肪及礦物質)之成分共同且與水一起組合於多種 漿料中。對此摻合物進行初始熱處理,接著進行測試以檢 驗適當營養素含量。 成分 用於調配強化劑之32,000 lbs之量 實例95 實例96 實例97 實例98 實例99 水 足量 足量 足量 足量 足量 玉米麥芽糊精 1450 kg 1450 kg 1450 kg 1450 kg 1450 kg 玉米糖漿固體 1388 kg 1388 kg 1388 kg 1388 kg 1388 kg 中鏈三酸甘油酯 694 kg 694 kg 694 kg 694 kg 694 kg 婉豆蛋白水解物 1944.4 kg 1847.18 kg 2041.6 kg 1750.0 kg 2138.8 kg 磷酸鈣 271kg 271kg 271kg 271kg 271kg 抗壞血酸 152 kg 152 kg 152 kg 152 kg 152 kg 158197.doc -68- 201212828Biotin 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg Folic acid 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg Cyanocobalamin 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg Ascorbic acid 153.5 kg 153.5 kg 153.5 kg 153.5 kg 153.5 kg Ingredients for Formulation of 8,172 kg of fortifier Example 90 Example 91 Example 92 Example 93 Example 94 Component water 16,205 L 16,205 L 16,205 L 16,205 L 16,205 L Corn syrup solid 1603 kg 1603 kg 1603 kg 1603 kg 1603 kg Magnesium chloride 96.2 kg 96.2 kg 96.2 kg 96.2 Kg 96.2 kg potassium citrate 223.8 kg 223.8 kg 223.8 kg 223.8 kg 223.8 kg sodium citrate 6.6 kg 6.6 kg 6.6 kg 6.6 kg 6.6 kg sodium chloride 15.4 kg 15.4 kg 15.4 kg 15.4 kg 15.4 kg MTC oil 801 kg 801 kg 801 Kg 801 kg 801 kg Egg squama 16.6 kg 16.6 kg 16.6 kg 16.6 kg 16.6 kg Vitamin A 2.36 kg 2.36 kg 2.36 kg 2.36 kg 2.36 kg Vitamin D 359.3 g 359.3 g 359.3 g 359.3 g 359.3 g Vitamin K 27.5 g 27.5 g 27.5 g 27.5 g 27.5 g Tocopherol acetate 15.8 kg 15.8 kg 15.8 kg 15.8 kg 15.8 kg Calcium carbonate 33.1 kg 33.1 kg 33.1 kg 33.1 kg 33.1kg Tricalcium phosphate 646 kg 646 kg 646 kg 646 kg 646 kg Cowpea protein hydrolysate 1348.3 kg 1537.0 kg 1981.9 kg 1941.5 kg 2669.58 kg Soy protein concentrate 753.0 kg 602.4 kg 451.8 kg 301.2 kg 150.6 kg skim milk powder 1653.5 kg 1322.8 Kg 992.1 kg 661.4 kg 3.31 kg Potassium citrate 257.2 g 257.2 g 257.2 g 257.2 g 257.2 g ferrous sulfate 3.7 kg 3.7 kg 3.7 kg 3.7 kg 3.7 kg Sulfuric acid 11.1 kg 11.1 kg 11.1 kg 11.1 kg 11.1 kg Copper sulfate 1.84 kg 1.84 Kg 1.84 kg 1.84 kg 1.84 kg sulfuric acid 0.320 kg 0.320 kg 0.320 kg 0.320 kg 0.320 kg sodium citrate 0.001 kg 0.001 kg 0.001 kg 0.001 kg 0.001 kg 158197.doc •67- 201212828 Nicotine decylamine 0.98 kg 0.98 kg 0.98 kg 0.98 Kg 0.98 kg riboflavin 1.14 kg 1.14 kg 1.14 kg 1.14 kg 1.14 kg calcium pantothenate 4.08 kg 4.08 kg 4.08 kg 4.08 kg 4.08 kg hydrochloric acid sterol 0.655 kg 0.655 kg 0.655 kg 0.655 kg 0.655 kg m-inositol 9.55 kg 9.55 Kg 9.55 kg 9.55 kg 9.55 kg Biotin 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg 0.0727 kg Folic acid 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg Cyanocobalamin 0. 0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg ascorbic acid 153.5 kg 153.5 kg 153.5 kg 153.5 kg 153.5 kg Examples 95-104 In these examples, a gelled human milk fortifier was prepared in accordance with the present invention. The ingredients of the gelled human milk fortifier are shown in the table below. A gelled human milk fortifier is prepared by dissolving and combining the ingredients in a homogeneous aqueous mixture which is suitably heat treated to achieve long-term storage stability. To begin the manufacturing process, a large amount of nutrients (carbohydrate, protein, fat, and minerals) are supplied together and combined with water in various slurries. The blend was subjected to an initial heat treatment followed by a test to check the appropriate nutrient content. Ingredients used to formulate 32,000 lbs of fortifier Example 95 Example 96 Example 97 Example 98 Example 99 Water sufficient amount sufficient sufficient sufficient amount of corn maltodextrin 1450 kg 1450 kg 1450 kg 1450 kg 1450 kg corn syrup solid 1388 kg 1388 kg 1388 kg 1388 kg 1388 kg Medium chain triglyceride 694 kg 694 kg 694 kg 694 kg 694 kg Cowpea protein hydrolysate 1944.4 kg 1847.18 kg 2041.6 kg 1750.0 kg 2138.8 kg Calcium phosphate 271kg 271kg 271kg 271kg 271kg Ascorbic acid 152 Kg 152 kg 152 kg 152 kg 152 kg 158197.doc -68- 201212828
氣化鎂 38.0 kg 38.0 kg 38.0 kg 38.0 kg 38.0 kg 檸檬酸鉀 12.2 kg 12.2 kg 12.2 kg 12.2 kg 12.2 kg 氣化納 12.1kg 12.1 kg 12.1 kg 12.1 kg 12.1 kg 大豆卵填脂 8.84 kg 8.84 kg 8.84 kg 8.84 kg 8.84 kg M-肌醇 7.98 kg 7.98 kg 7.98 kg 7.98 kg 7.98 kg 磷酸鎂 5.55 kg 5.55 kg 5.55 kg 5.55 kg 5.55 kg 高山被孢黴油 5.35 kg 5.35 kg 5.35 kg 5.35 kg 5.35 kg 菸鹼醯胺 4.35 kg 4.35 kg 4.35 kg 4.35 kg 4.35 kg α-生育酚乙酸酯 4.21 kg 4.21 kg 4.21 kg 4.21 kg 4.21 kg 硫酸辞 3.51 kg 3.51 kg 3.51 kg 3.51 kg 3.51 kg 藻類隱曱藻油 3.45 kg 3.45 kg 3.45 kg 3.45 kg 3.45 kg 氣化膽驗 2.90 kg 2.90 kg 2.90 kg 2.90 kg 2.90 kg 泛酸鈣 1.89 kg 1.89 kg 1.89 kg 1.89 kg 1.89 kg 磷酸鉀 1.62 kg 1.62 kg 1.62 kg 1.62 kg 1.62 kg 硫酸亞鐵 1.64 kg 1.64 kg 1.64 kg 1.64 kg 1.64 kg 維生素Α棕橺酸酯 900 g 900 g 900 g 900 g 900 g 硫酸銅 678 g 678 g 678 g 678 g 678 g 核黃素 572 g 572 g 572 g 572 g 572 g 鹽酸硫胺素 373 g 373 g 373 g 373 g 373 g 鹽酸°比哆醇 232 g 232 g 232 g 232 g 232 g 維生素D3 152 g 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 葉酸 47.9 g 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 生物素 34.1 g 153.5 kg 153.5 kg 153.5 kg 153.5 kg 硫酸猛· 23.2 g 23.2 g 23.2 g 23.2 g 23.2 g 葉綠醌 11.6g 11.6 g 11.6g 11.6 g 11.6g 氰鈷胺 1.60 g 1.60 g 1.60 g 1.60 g 1.60 g 砸酸納 0.798 g 0.798 g 0.798 g 0.798 g 0.798 g 碳酸鈣 按需要 按需要 按需要 按需要 按需要 檸檬酸鈉 按需要 按需要 按需要 按需要 按需要 氫氧化鉀 按需要 按需要 按需要 按需要 按需要 158197.doc -69- 201212828 成分 用於調配強化劑之32,000丨bs之量 實例100 實例101 實例102 實例103 實例104 水 足量 足量 足量 足量 足量 濃縮脫脂乳 2625 kg 2100 kg 1575 kg 1050 kg 525 kg 脫脂乳固體 682.5 kg 546 kg 409.5 kg 273 kg 136.5 kg 玉米麥芽糊精 1450 kg 1450 kg 1450 kg 1450 kg 1450 kg 玉米糖漿固體 1388 kg 1388 kg 1388 kg 1388 kg 1388 kg 中鏈三酸甘油酉旨 694 kg 694 kg 694 kg 694 kg 694 kg 婉豆蛋白水解物 972.2 kg 1166.6 kg 1361.1kg 1555.5 kg 1750.0 kg 乳清蛋白濃縮物 317 kg 253.6 kg 190.2 kg 126.8 kg 63.4 kg 磷酸鈣 271kg 271 kg 271kg 271kg 271kg 抗壞血酸 152 kg 152 kg 152 kg 152 kg 152 kg 氣化鎂 38.0 kg 38.0 kg 38.0 kg 38.0 kg 38.0 kg 擰檬酸鉀 12.2 kg 12.2 kg 12.2 kg 12.2 kg 12.2 kg 氯化鈉 12.1 kg 12.1 kg 12.1 kg 12.1kg 12.1 kg 大豆卵鱗脂 8.84 kg 8.84 kg 8.84 kg 8.84 kg 8.84 kg M-肌醇 7.98 kg 7.98 kg 7.98 kg 7.98 kg 7.98 kg 磷酸鎂 5.55 kg 5.55 kg 5.55 kg 5.55 kg 5.55 kg 高山被孢黴油 5.35 kg 5.35 kg 5.35 kg 5.35 kg 5.35 kg 菸鹼醯胺 4.35 kg 4.35 kg 4.35 kg 4.35 kg 4.35 kg α-生育驗乙酸酉旨 4.21 kg 4.21 kg 4.21 kg 4.21 kg 4.21 kg 硫酸辞 3.51 kg 3.51 kg 3.51 kg 3.51 kg 3.51 kg 藻類隱曱藻油 3.45 kg 3.45 kg 3.45 kg 3.45 kg 3.45 kg 氣化膽驗 2.90 kg 2.90 kg 2.90 kg 2.90 kg 2.90 kg 泛酸鈣 1.89 kg 1.89 kg 1.89 kg 1.89 kg 1.89 kg 磷酸鉀 1.62 kg 1.62 kg 1.62 kg 1.62 kg 1.62 kg 硫酸亞鐵 1.64 kg 1.64 kg 1.64 kg 1.64 kg 1.64 kg 維生素Α棕櫊酸酯 900 g 900 g 900 g 900 g 900 g 158197.doc •70- 201212828 硫酸銅 678 g 678 g 678 g 678 g 678 g 核黃素 572 g 572 g 572 g 572 g 572 g 鹽酸硫胺素 373 g 373 g 373 g 373 g 373 g 鹽酸η比哆醇 232 g 232 g 232 g 232 g 232 g 維生素D3 152 g 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg 葉酸 47.9 g 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg 生物素 34.1 g 153.5 kg 153.5 kg 153.5 kg 153.5 kg 硫酸锰 23.2 g 23.2 g 23.2 g 23.2 g 23.2 g 葉綠醌 11.6 g 11.6g 11.6g 11.6g 11.6g 氰鈷胺 1.60 g 1.60 g 1.60 g 1.60 g 1.60 g 石西酸納 0.798 g 0.798 g 0.798 g 0.798 g 0.798 g 碳酸鈣 按需要 按需要 按需要 按需要 按需要 檸檬酸鈉 按需要 按需要 按需要 按需要 按需要 氫氧化鉀 按需要 按需要 按需要 按需要 按需要Magnesium vapor 38.0 kg 38.0 kg 38.0 kg 38.0 kg 38.0 kg Potassium citrate 12.2 kg 12.2 kg 12.2 kg 12.2 kg 12.2 kg Gasification sodium 12.1 kg 12.1 kg 12.1 kg 12.1 kg 12.1 kg Soybean egg fat 8.84 kg 8.84 kg 8.84 kg 8.84 Kg 8.84 kg M-inositol 7.98 kg 7.98 kg 7.98 kg 7.98 kg 7.98 kg Magnesium phosphate 5.55 kg 5.55 kg 5.55 kg 5.55 kg 5.55 kg Algae sp. oil 5.35 kg 5.35 kg 5.35 kg 5.35 kg 5.35 kg Nicotine guanamine 4.35 kg 4.35 kg 4.35 kg 4.35 kg 4.35 kg α-tocopherol acetate 4.21 kg 4.21 kg 4.21 kg 4.21 kg 4.21 kg Sulfuric acid 3.51 kg 3.51 kg 3.51 kg 3.51 kg 3.51 kg Algae cryptic algae oil 3.45 kg 3.45 kg 3.45 kg 3.45 kg 3.45 kg gasification bile test 2.90 kg 2.90 kg 2.90 kg 2.90 kg 2.90 kg calcium pantothenate 1.89 kg 1.89 kg 1.89 kg 1.89 kg 1.89 kg potassium phosphate 1.62 kg 1.62 kg 1.62 kg 1.62 kg 1.62 kg ferrous sulfate 1.64 kg 1.64 kg 1.64 kg 1.64 Kg 1.64 kg Vitamin Α palmitate 900 g 900 g 900 g 900 g 900 g Copper sulfate 678 g 678 g 678 g 678 g 678 g Riboflavin 572 g 572 g 572 g 572 g 572 g Thiamine hydrochloride 373 g 373 g 373 g 373 g 373 g salt °Comparinol 232 g 232 g 232 g 232 g 232 g Vitamin D3 152 g 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg Folic acid 47.9 g 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg Biotin 34.1 g 153.5 kg 153.5 kg 153.5 kg 153.5 kg Sulfuric acid Meng 23.2 g 23.2 g 23.2 g 23.2 g 23.2 g chlorophyllin 11.6g 11.6 g 11.6g 11.6 g 11.6g cyanocobalamin 1.60 g 1.60 g 1.60 g 1.60 g 1.60 g sodium citrate 0.798 g 0.798 g 0.798 g 0.798 g 0.798 g Calcium carbonate as needed, as needed, as needed, sodium citrate, as needed, as needed, as needed, potassium hydroxide, as needed, as needed, as needed, as needed 158197.doc -69- 201212828 ingredients for blending Example of amount of enhancer 32,000 丨bs Example 100 Example 101 Example 102 Example 103 Example 104 Water sufficient amount sufficient amount sufficient sufficient amount of concentrated skim milk 2625 kg 2100 kg 1575 kg 1050 kg 525 kg skim milk solid 682.5 kg 546 kg 409.5 Kg 273 kg 136.5 kg corn maltodextrin 1450 kg 1450 kg 1450 kg 1450 kg 1450 kg corn syrup solid 1388 kg 1388 kg 1388 kg 1388 kg 1388 kg medium chain three Glycerin 694 694 kg 694 kg 694 kg 694 kg 694 kg Cowpea protein hydrolysate 972.2 kg 1166.6 kg 1361.1kg 1555.5 kg 1750.0 kg Whey protein concentrate 317 kg 253.6 kg 190.2 kg 126.8 kg 63.4 kg Calcium phosphate 271kg 271 kg 271kg 271kg 271kg ascorbic acid 152 kg 152 kg 152 kg 152 kg 152 kg gasification magnesium 38.0 kg 38.0 kg 38.0 kg 38.0 kg 38.0 kg potassium citrate 12.2 kg 12.2 kg 12.2 kg 12.2 kg 12.2 kg sodium chloride 12.1 kg 12.1 kg 12.1 kg 12.1kg 12.1 kg Soybean egg fat 8.84 kg 8.84 kg 8.84 kg 8.84 kg 8.84 kg M-inositol 7.98 kg 7.98 kg 7.98 kg 7.98 kg 7.98 kg Magnesium phosphate 5.55 kg 5.55 kg 5.55 kg 5.55 kg 5.55 kg Alkali spore oil 5.35 kg 5.35 Kg 5.35 kg 5.35 kg 5.35 kg Nicotine guanamine 4.35 kg 4.35 kg 4.35 kg 4.35 kg 4.35 kg α-fertility acetate test 4.21 kg 4.21 kg 4.21 kg 4.21 kg 4.21 kg Sulfuric acid 3.51 kg 3.51 kg 3.51 kg 3.51 kg 3.51 kg Algae cryptic algae oil 3.45 kg 3.45 kg 3.45 kg 3.45 kg 3.45 kg Gasification biliary test 2.90 kg 2.90 kg 2.90 kg 2.90 kg 2.90 kg Calcium pantothenate 1.89 kg 1.89 kg 1.89 kg 1.89 kg 1.89 kg Potassium phosphate 1.6 2 kg 1.62 kg 1.62 kg 1.62 kg 1.62 kg Ferrous sulfate 1.64 kg 1.64 kg 1.64 kg 1.64 kg 1.64 kg Vitamin Α palmitate 900 g 900 g 900 g 900 g 900 g 158197.doc •70- 201212828 Copper sulphate 678 g 678 g 678 g 678 g 678 g riboflavin 572 g 572 g 572 g 572 g 572 g thiamine hydrochloride 373 g 373 g 373 g 373 g 373 g η sterol hydrochloride 232 g 232 g 232 g 232 g 232 g Vitamin D3 152 g 0.0775 kg 0.0775 kg 0.0775 kg 0.0775 kg Folic acid 47.9 g 0.0016 kg 0.0016 kg 0.0016 kg 0.0016 kg Biotin 34.1 g 153.5 kg 153.5 kg 153.5 kg 153.5 kg Manganese sulfate 23.2 g 23.2 g 23.2 g 23.2 g 23.2 g Leaf green 醌11.6 g 11.6g 11.6g 11.6g 11.6g cyanocobalamin 1.60 g 1.60 g 1.60 g 1.60 g 1.60 g sodium sulphate 0.798 g 0.798 g 0.798 g 0.798 g 0.798 g calcium carbonate as needed Sodium sulphate as needed, as needed, as needed, potassium hydroxide, as needed, as needed, as needed
實例 105-114 實例105-114說明本發明之營養粉,其成分列於下表 中。藉由喷霧乾燥法以各別批次製備此等產品,在使用之 前用水復原至所需目標成分濃度。除非另外規定,否則所 有成分量均以每1 〇〇〇 kg批量之產品的千克數列出。 成分 每1000 kg 之量(kg) 實例105 實例106 實例107 實例108 實例109 麥特靈(maltrin) 436.7 436.7 436.7 436.7 436.7 蔗糖 145.5 145.5 145.5 145.5 145.5 豌豆蛋白水解物 186.8 177.5 196.14 168.12 205.5 共軛亞油酸 67.0 63.65 70.35 60.3 73.7 FOS粉 33.6 33.6 33.6 33.6 33.6 高油酸葵花油 33.1 33.1 33.1 33.1 33.1 158197.doc -71 - 201212828 β-羥基-β-曱基丁酸鈣 31.6 31.6 31.6 31.6 31.6 芥花油 28.3 28.3 28.3 28.3 28.3 大豆油 13.3 13.3 13.3 13.3 13.3 檸檬酸鉀 10.3 10.3 10.3 10.3 10.3 檸檬酸鈉 5.8 5.8 5.8 5.8 5.8 氣化鉀 5.2 5.2 5.2 5.2 5.2 氣化鎂 4.7 4.7 4.7 4.7 4.7 氫氧化鉀 3.1 3.1 3.1 3.1 3.1 磷酸氳二鈉脫水物 3.0 3.0 3.0 3.0 3.0 氣化納 2.5 2.5 2.5 2.5 2.5 氣化膽鹼 1.8 1.8 1.8 1.8 1.8 天然及人造香草調味劑 1.8 1.8 1.8 1.8 1.8 單水合磷酸二氫鈉 1.6 1.6 1.6 1.6 1.6 三水合鱗酸氫二鉀 1.1 1.1 1.1 1.1 1.1 人造棉花糖調味劑 1.0 1.0 1.0 1.0 1.0 維生素預混物 1.0 1.0 1.0 1.0 1.0 棕櫚酸抗壞血酸酯 0.2437 0.2437 0.2437 0.2437 0.2437 抗壞血酸 0.2400 0.2400 0.2400 0.2400 0.2400 生育酚-2食品級抗氧化劑 0.1161 0.1161 0.1161 0.1161 0.1161 硫酸亞鐵 0.0929 0.0929 0.0929 0.0929 0.0929 維生素預混物 0.065 0.065 0.065 0.065 0.065 單水合硫酸鋅 0.057 0.057 0.057 0.057 0.057 硫酸猛 0.0454 0.0454 0.0454 0.0454 0.0454 礦物質混合硫酸銅 0.0353 0.0353 0.0353 0.0353 0.0353 30% Ρ胡蘿蔔素 0.0056 0.0056 0.0056 0.0056 0.0056 氣化鉻 0.0013 0.0013 0.0013 0.0013 0.0013 鉬酸鈉 0.0012 0.0012 0.0012 0.0012 0.0012 块化鉀 0.001 0.001 0.001 0.001 0.001 硒酸鈉 0.0004008 0.0004008 0.0004008 0.0004008 0.0004008 158197.doc -72- 201212828 檸檬酸 按需要 按需要 按需要 按需要 按需要 氫氧化鉀 按需要 按需要 按需要 按需要 按需要 無水硫酸鎂 按需要 按需要 按需要 按需要 按需要 超微米尺寸化礙酸三鈣 按需要 按需要 按需要 按需要 按需要 抗壞血酸 按需要 按需要 按需要 按需要 按需要 水 足量 足量 足量 足量 足量Examples 105-114 Examples 105-114 illustrate nutritional powders of the present invention, the ingredients of which are listed in the following table. These products are prepared in separate batches by spray drying and are reconstituted with water to the desired target ingredient concentration prior to use. All ingredients are listed in kilograms per 1 〇〇〇 kg lot unless otherwise stated. Ingredient per 1000 kg (kg) Example 105 Example 106 Example 107 Example 108 Example 109 maltrin 436.7 436.7 436.7 436.7 436.7 Sucrose 145.5 145.5 145.5 145.5 145.5 Pea protein hydrolysate 186.8 177.5 196.14 168.12 205.5 Conjugated linoleic acid 67.0 63.65 70.35 60.3 73.7 FOS powder 33.6 33.6 33.6 33.6 33.6 High oleic sunflower oil 33.1 33.1 33.1 33.1 33.1 158197.doc -71 - 201212828 β-Hydroxy-β-mercaptobutyrate 31.6 31.6 31.6 31.6 31.6 Canola oil 28.3 28.3 28.3 28.3 28.3 Soybean oil 13.3 13.3 13.3 13.3 13.3 Potassium citrate 10.3 10.3 10.3 10.3 10.3 Sodium citrate 5.8 5.8 5.8 5.8 5.8 Calcium hydride 5.2 5.2 5.2 5.2 5.2 Magnesium 4.7 4.7 4.7 4.7 4.7 Potassium hydroxide 3.1 3.1 3.1 3.1 3.1 Bismuth disodium phosphate dehydrate 3.0 3.0 3.0 3.0 3.0 Gasification nano 2.5 2.5 2.5 2.5 2.5 Gasification choline 1.8 1.8 1.8 1.8 1.8 Natural and artificial vanilla flavoring 1.8 1.8 1.8 1.8 1.8 Sodium dihydrogen phosphate monohydrate 1.6 1.6 1.6 1.6 1.6 Dipotassium bisulphate trihydrate 1.1 1.1 1.1 1.1 1.1 Artificial Marshmallow Flavor 1.0 1.0 1.0 1.0 1.0 Vitamin Premix 1.0 1.0 1.0 1.0 1.0 Ascorbyl palmitate 0.2437 0.2437 0.2437 0.2437 0.2437 Ascorbic acid 0.2400 0.2400 0.2400 0.2400 0.2400 Tocopherol-2 food grade antioxidant 0.1161 0.1161 0.1161 0.1161 0.1161 Ferrous sulfate 0.0929 0.0929 0.0929 0.0929 0.0929 Vitamin premix 0.065 0.065 0.065 0.065 0.065 Monohydrate sulfuric acid Zinc 0.057 0.057 0.057 0.057 0.057 Sulfuric acid 0.0454 0.0454 0.0454 0.0454 0.0454 Mineral mixed copper sulfate 0.0353 0.0353 0.0353 0.0353 0.0353 30% Beta carotene 0.0056 0.0056 0.0056 0.0056 0.0056 Calcined chromium 0.0013 0.0013 0.0013 0.0013 0.0013 Sodium molybdate 0.0012 0.0012 0.0012 0.0012 0.0012 Potassium 0.001 0.001 0.001 0.001 0.001 0.001 Sodium sulphate 0.00004008 0.0004008 0.0004008 0.0004008 0.0004008 158197.doc -72- 201212828 Citric acid as needed, as needed, as needed, potassium hydroxide, as needed, as needed, as needed, as needed, anhydrous magnesium sulfate As needed, as needed, as needed, ultra-micron size, acid tricalcium, as needed, as needed, as needed, as needed Acid as required as required as required as required as required plenty of water enough enough enough enough
成分 每1000 kg之量(kg) 實例110 實例Π1 實例112 實例113 實例114 麥芽糊精 436.7 436.7 436.7 436.7 436.7 蔗糖 145.5 145.5 145.5 145.5 145.5 酪蛋白鈣 64.6 51.6 38.7 25.8 12.91 婉豆蛋白水解物 93.4 112.1 130.8 149.4 168.1 共輊亞油酸 67.0 63.65 70.35 60.3 73.7 大豆蛋白分離物 28.8 23.1 17.3 11.5 5.77 FOSS 33.6 33.6 33.6 33.6 33.6 高油酸葵花油 33.1 33.1 33.1 33.1 33.1 P-羥基-P-曱基丁酸妈 31.6 31.6 31.6 31.6 31.6 芬花油 28.3 28.3 28.3 28.3 28.3 大豆油 13.3 13.3 13.3 13.3 13.3 檸檬酸鉀 10.3 10.3 10.3 10.3 10.3 擰檬酸鈉 5.8 5.8 5.8 5.8 5.8 氣化钟 5.2 5.2 5.2 5.2 5.2 氣化鎂 4.7 4.7 4.7 4.7 4.7 氫氧化鉀 3.1 3.1 3.1 3.1 3.1 磷酸氫二鈉脫水物 3.0 3.0 3.0 3.0 3.0 氣化納 2.5 2.5 2.5 2.5 2.5 氣化膽鹼 1.8 1.8 1.8 1.8 1.8 天然及人造香草調味劑 1.8 1.8 1.8 1.8 1.8 158197.doc •73- 201212828 單水合鱗酸二氫鈉 1.6 1.6 1.6 1.6 1.6 三水合磷酸氫二鉀 1.1 1.1 1.1 1.1 1.1 人造棉花糖調味劑 1.0 1.0 1.0 1.0 1.0 維生素預混物 1.0 1.0 1.0 1.0 1.0 棕櫚酸抗壞血酸酯 0.2437 0.2437 0.2437 0.2437 0.2437 抗壞血酸 0.2400 0.2400 0.2400 0.2400 0.2400 生育酚-2食品級抗氧化劑 0.1161 0.1161 0.1161 0.1161 0.1161 硫酸亞鐵 0.0929 0.0929 0.0929 0.0929 0.0929 維生素預混物 0.065 0.065 0.065 0.065 0.065 單水合硫酸辞 0.057 0.057 0.057 0.057 0.057 硫酸錳 0.0454 0.0454 0.0454 0.0454 0.0454 礦物質混合硫酸銅 0.0353 0.0353 0.0353 0.0353 0.0353 30% p胡蘿蔔素 0.0056 0.0056 0.0056 0.0056 0.0056 氣化鉻 0.0013 0.0013 0.0013 0.0013 0.0013 鉬酸鈉 0.0012 0.0012 0.0012 0.0012 0.0012 块化鉀 0.001 0.001 0.001 0.001 0.001 硒酸鈉 0.0004008 0.0004008 0.0004008 0.0004008 0.0004008 檸檬酸 按需要 按需要 按需要 按需要 按需要 氫氧化鉀 按需要 按需要 按需要 按需要 按需要 無水硫酸鎂 按需要 按需要 按需要 按需要 按需要 超微米尺寸化磷酸三鈣 按需要 按需要 按需要 按需要 按需要 抗壞血酸 按需要 按需要 按需要 按需要 按需要 水 足量 足量 足量 足量 足量 【圖式簡單說明】 圖1為描述本發明之豌豆蛋白水解物與完整大豆蛋白之 間的組成比較的圖表。 158197.doc -74-Ingredients per 1000 kg (kg) Example 110 Example Π 1 Example 112 Example 113 Example 114 Maltodextrin 436.7 436.7 436.7 436.7 436.7 Sucrose 145.5 145.5 145.5 145.5 145.5 Casein calcium 64.6 51.6 38.7 25.8 12.91 Bean protein hydrolysate 93.4 112.1 130.8 149.4 168.1 Co-linoleic acid 67.0 63.65 70.35 60.3 73.7 Soy protein isolate 28.8 23.1 17.3 11.5 5.77 FOSS 33.6 33.6 33.6 33.6 33.6 High oleic sunflower oil 33.1 33.1 33.1 33.1 33.1 P-hydroxy-P-mercaptobutyric acid 31.6 31.6 31.6 31.6 31.6 Fennel oil 28.3 28.3 28.3 28.3 28.3 Soybean oil 13.3 13.3 13.3 13.3 13.3 Potassium citrate 10.3 10.3 10.3 10.3 10.3 Sodium citrate 5.8 5.8 5.8 5.8 5.8 Gasification clock 5.2 5.2 5.2 5.2 5.2 Magnesium 4.7 4.7 4.7 4.7 4.7 Potassium hydroxide 3.1 3.1 3.1 3.1 3.1 Disodium hydrogen phosphate dehydrate 3.0 3.0 3.0 3.0 3.0 Gasification nano 2.5 2.5 2.5 2.5 2.5 Gasification choline 1.8 1.8 1.8 1.8 1.8 Natural and artificial vanilla flavoring 1.8 1.8 1.8 1.8 1.8 158197. Doc •73- 201212828 Sodium dihydrogen sulphate monohydrate 1.6 1.6 1.6 1.6 1.6 Dipotassium hydrogen phosphate trihydrate 1.1 1.1 1.1 1.1 1.1 Marshmallow Flavor 1.0 1.0 1.0 1.0 1.0 Vitamin Premix 1.0 1.0 1.0 1.0 1.0 Ascorbyl palmitate 0.2437 0.2437 0.2437 0.2437 0.2437 Ascorbic acid 0.2400 0.2400 0.2400 0.2400 0.2400 Tocopherol-2 food grade antioxidant 0.1161 0.1161 0.1161 0.1161 0.1161 Ferrous sulfate 0.0929 0.0929 0.0929 0.0929 0.0929 Vitamin premix 0.065 0.065 0.065 0.065 0.065 Monohydrate sulfuric acid 0.057 0.057 0.057 0.057 0.057 Manganese sulfate 0.0454 0.0454 0.0454 0.0454 0.0454 Mineral mixed copper sulfate 0.0353 0.0353 0.0353 0.0353 0.0353 30% p carotene 0.0056 0.0056 0.0056 0.0056 0.0056 Chromium oxide 0.0013 0.0013 0.0013 0.0013 0.0013 Sodium molybdate 0.0012 0.0012 0.0012 0.0012 0.0012 Potassium 0.001 0.001 0.001 0.001 0.001 Sodium selenate 0.00004008 0.0004008 0.0004008 0.0004008 0.0004008 Citric acid as needed, as needed, as needed, potassium hydroxide, as needed Need to be as needed, as needed, anhydrous magnesium sulfate, as needed, as needed, ultra-micronized tricalcium phosphate as needed As needed, as needed, as needed, ascorbic acid as needed, as needed, as needed, as needed, sufficient amount of sufficient amount of sufficient amount. [Simplified illustration] Figure 1 is a description of the pea protein hydrolysate and intact soybean of the present invention. A chart comparing the composition of proteins. 158197.doc -74-
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DE69220568T2 (en) * | 1991-03-07 | 1997-11-13 | Novonordisk As | PROTEIN HYDROLYSATE FROM PEA, METHOD FOR THE PRODUCTION AND USE THEREOF |
US5514656A (en) * | 1993-05-28 | 1996-05-07 | Abbott Laboratories | Method of providing enteral nutritional support for patients undergoing radiation therapy and/or chemotherapy |
ES2219871T3 (en) | 1997-02-21 | 2004-12-01 | Abbott Laboratories | PROCEDURE AND COMPOSITIONS THAT SERVE TO LIMIT THE FREQUENCY OF NECROTIZING ENTEROCOLITIS OF THE NEWBORN. |
US6365218B1 (en) | 2000-02-04 | 2002-04-02 | Abbott Laboratories | Pediatric formula and methods for providing nutrition and improving tolerance |
US6811801B2 (en) | 2001-12-12 | 2004-11-02 | Abbott Laboratories | Methods and compositions for brightening the color of thermally processed nutritionals |
US7070825B2 (en) * | 2002-09-10 | 2006-07-04 | Abbott Laboratories | Infant formula |
US20060286208A1 (en) * | 2005-06-01 | 2006-12-21 | Nagendra Rangavajla | Methods for producing protein partial hydrolysates and infant formulas containing the same |
EP1911457A1 (en) * | 2006-10-12 | 2008-04-16 | N.V. Nutricia | Composition to treat or prevent gastrointestinal infection |
US8197872B2 (en) | 2007-05-17 | 2012-06-12 | The Regents Of The University Of California | Human milk oligosaccharides to promote growth of beneficial gut bacteria |
-
2011
- 2011-08-22 WO PCT/US2011/048665 patent/WO2012027287A1/en active Application Filing
- 2011-08-24 TW TW100130367A patent/TW201212828A/en unknown
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108601371A (en) * | 2016-01-29 | 2018-09-28 | 罗盖特公司 | Include the nutraceutical formulation of pea protein isolate |
CN108601371B (en) * | 2016-01-29 | 2023-08-08 | 罗盖特公司 | Nutritional formulations comprising pea protein isolate |
CN114395600A (en) * | 2022-01-14 | 2022-04-26 | 琛蓝(美国)营养制品股份有限公司 | Preparation method and application of multifunctional pea peptide |
CN114395600B (en) * | 2022-01-14 | 2023-12-12 | 琛蓝(美国)营养制品股份有限公司 | Preparation method and application of multifunctional pea peptide |
Also Published As
Publication number | Publication date |
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WO2012027287A1 (en) | 2012-03-01 |
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