201208732 六、發明說明： 【發明所屬之技術領域】 本發明是有關於一種牙齒保健的方法，且特別是有關於一種 增進牙周組織細胞活性的方法。 【先前技術】 請參照圖1，圖1係繪示一人類牙齒10及牙周組織12的結構 剖面圖。一般而言，牙周組織12通指牙齒周圍支持的組織，包括 了牙周韌帶121、齒槽骨122、牙齦123及牙骨質124等，牙周組 織是用作支特及穩固牙齒的組織，使牙齒能發揮最有效的咀嚼功 能。牙周組織的損壞’其中最常見的症狀包括了牙齦發炎 (gingivitis)、牙齦與齒槽骨的萎縮(gingivai血叩如)。這些缺陷時常 會造成美觀上的問題、敏感以及牙齒搖動的困擾，長時間下來就 很容易轉變成牙周病（periodontal disease) ^目前醫學研究中指出，牙周組織12的損壞多是由存留在牙頸 =(dental neck)的牙菌斑(dental plaqUe)所引起。當牙菌斑開始釋放 毋素時，就會侵犯牙齒周圍的支持組織，當這些細菌大量繁殖蔓 ，，就會開始造成牙齦的疼痛，嚴重的時候會化膿(suppu她η)， 若不處理、持續惡化，牙齒_著的胁會慢慢流失，牙 搖動，最後牙齒就會逐一脫落。 α 現有的牙醫技術對於組_壞的處理，基本上是以降低口 腔内的細菌及減少牙周組織(牙齦、牙_帶等)的發氮 常採用手術刮除口腔内的細菌、搭配抗生素及消’·。 亦或在治療的同時，以雷射光照射口腔、 制生物膜(biofllm)形成。 賴杈菌及抑 201208732 然而，手術到除治療具有侵入性 極 作，且容易產生，例如物萎 翻㈣直接進仃刼 遺症。而雷射_高能量，根暴露，對冷減感等後 的生長，但近距離長時間二然:=腔==生物膜 〇 ra u. A θ對牙齒、牙周或其他口腔組 織^祕。因此’需輯崎能量 或專業人員指導下進行操作師 用。 罘作並不適合作為日常預防保健使 Ϊίί提供—種不會對牙齒、牙周或其他口腔_ U去έ 4口0供一般人日常牙齒預防保養的方法，以達到 保健牙齒組織的目的。 【發明内容】 有4α於此’本發明係提供—種增進？周組織細胞活性的方 法，包括以至少一個發光二極體(Ught Emitting Di〇de，led) 光源，照射口腔區域中至少一顆牙齒的牙周組織，其照射能量 介於0·1焦、耳/平方公分(J/cm2)到1〇焦耳/平方公分之間。 在本發明的—實施例中，牙周組織包含齒槽骨(alveolar b〇ne):細f ’發光二極體光源之照紐為415±25奈雜m)，且 照射能$介於〇·2焦耳/平方公分到5焦耳/平方公分之間。 一在本發明的一實施例中，牙周組織包含齒槽骨細胞，發光 一極體光源之照光波長為575±25奈米，且照射能量介於1焦 耳/平方公分到1〇焦耳/平方公分之間。 在本發明的一實施例中，牙周組織包含齒槽骨細胞，發光 一極體光源之照光波長為635±25奈米’且照射能量介於1焦 耳/平方公分到1〇焦耳/平方公分之間。 在本發明的一實施例中，牙周組織包含牙齦纖維母細胞201208732 VI. Description of the Invention: [Technical Field] The present invention relates to a method of dental care, and more particularly to a method for enhancing cell activity of periodontal tissues. [Prior Art] Referring to Figure 1, Figure 1 is a cross-sectional view showing the structure of a human tooth 10 and a periodontal tissue 12. In general, the periodontal tissue 12 refers to the tissue supported around the teeth, including the periodontal ligament 121, the alveolar bone 122, the gums 123, and the cementum 124. The periodontal tissue is used as a tissue for stinging and stabilizing the teeth. Allows the teeth to perform the most effective chewing function. The most common symptoms of periodontal tissue damage include gingivitis, atrophy of the gums and alveolar bone (gingivai blood stasis). These defects often cause aesthetic problems, sensitivity, and tooth shaking. It is easy to turn into periodontal disease after a long time. At present, medical research indicates that the damage of periodontal tissue 12 is mostly caused by Caused by dental plaque (dental plaqUe). When plaque begins to release alizarin, it will invade the supporting tissues around the teeth. When these bacteria multiply the vines, they will start to cause pain in the gums. In severe cases, they will purulent (suppu her η), if not processed, Continue to deteriorate, the teeth will slowly lose, the teeth will shake, and finally the teeth will fall off one by one. α The existing dental technology for the group _ bad treatment, basically to reduce the bacteria in the mouth and reduce the periodontal tissue (gum, teeth, etc.) of the nitrogen is often used to surgically scrape the bacteria in the mouth, with antibiotics and Eliminate '·. Or, at the same time as the treatment, the oral cavity and the biofllm are irradiated with laser light. Lai bacterium and depression 201208732 However, surgery to the treatment of invasive extremes, and easy to produce, such as vaginal (four) direct sequelae. And laser _ high energy, root exposure, growth after cooling, etc., but close distance for a long time: = cavity == biofilm 〇ra u. A θ on teeth, periodontal or other oral tissue . Therefore, it is necessary to operate the operator under the guidance of Zasaki Energy or a professional. It is not suitable for daily preventive care. Ϊίί provides a method for daily teeth prevention and maintenance for the teeth, periodontal or other oral cavity, for the purpose of health care. SUMMARY OF THE INVENTION There is a 4α in this invention provided by the invention? A method for organizing cell activity, comprising irradiating a periodontal tissue of at least one tooth in an oral region with at least one Ught Emitting Di〇de (led) light source, the irradiation energy being between 0.1 and 1 joule, ear / square centimeter (J / cm2) to 1 〇 joule / square centimeter. In the embodiment of the present invention, the periodontal tissue comprises alveolar b〇ne: the thin f 'light emitting diode light source is 415±25 nanometer m), and the irradiation energy is between 〇 • 2 joules per square centimeter to 5 joules per square centimeter. In an embodiment of the invention, the periodontal tissue comprises alveolar bone cells, and the illumination wavelength of the illuminating one-pole source is 575±25 nm, and the irradiation energy is between 1 Joule/cm 2 and 1 〇 Joule/square. Between the cents. In an embodiment of the invention, the periodontal tissue comprises alveolar bone cells, and the illumination wavelength of the illuminating one-pole light source is 635±25 nm and the irradiation energy is between 1 Joule/cm 2 and 1 〇 Joule/cm 2 . between. In an embodiment of the invention, the periodontal tissue comprises gingival fibroblasts
S 4 201208732 (Gingival Fibroblast’GF)，發光二極體光源之照光波為415±25 奈米，且照射能量介於1焦耳/平方公分到5焦耳/平方公分之 間。 A刀 在本發明的一實施例中，牙周組織包含牙齦纖維母細胞， 發光二極體光源之照光波長為575±25奈米，且照射能量介於 1焦耳/平方公分到10焦耳/平方公分之間。 在本發明的一實施例中，牙周組織包含牙齦纖維母細胞， 發光一極體光源之照光波長為635±25奈米，且照射能量介於 1焦耳/平方公分到10焦耳/平方公分之間。 在本發明的一實施例中，牙周組織包含牙周纖維母細胞 (Periodontal Fibroblast，PF)，發光二極體光源之照光波為 415±25奈米，且照射能量介於1焦耳/平方公分到5焦耳/平方 公分之間。 ^ 本發明的一實施例中，牙周組織包含牙周纖維母細胞，發 光一極體光源之照光波長為575±25奈米，且照射能量介於1 焦耳/平方公分到10焦耳/平方公分之間。 本發明的一實施例中，牙周組織包含牙周纖維母細胞，發 光一極體光源之照光波長為635士25奈米，且照射能量介於1 焦耳/平方公分到10焦耳/平方公分之間。 本發明的一實施例中，牙周組織包含牙周韌帶 (Periodontal Ligmam，PL)細胞，發光二極體光源之照光波 為415±25奈米，且照射能量介於〇.2焦耳/平方公分到$焦耳 /平方公分之間。 … 本發明的一實施例中，牙周組織包含牙周韌帶細胞，發光 二極體光源之照光波長為575±25奈米，且照射能量介於〇.2 焦耳/平方公分到5焦耳/平方公分之間。 201208732 l 一本發明的一實施例中，牙周组織包含牙周韌帶細胞，該發 光一極體光源之照光波長為63 5± 2 5奈米，且照射能量介於〇. 5 焦耳/平方公分到6焦耳/平方公分之間9 本發明的一實施例中，包括以複數個發光二極體光源，同 時…、射σ腔區域巾的複數顆牙齒的牙周組織，且相鄰兩發光二 極體光源所發出的光’照射範圍有部分重疊，使每顆牙齒的牙 周組織的照射能量均介於焦耳/平方公分到6焦耳/平方公 分之間。 於牙t Γ的—實關中’發光三極體光源的照射位置，係在 於牙齒與此牙齒之牙齦的交接處。 你 照射施例，树_提出—種㈣具有狀波長與 周組織細胞活性。由於所搡用組織進仃照射’以增進牙 低，直接昭身十光尤各似。秘光-級體光源，輻射能量較 適合提、牙周或其他口腔組織造成損傷， ^〇 巾齒預防保養，可達到保健牙周組織的目 為讓本發明之上述和其他目的、 ，下文特舉較佳實施例，並配合所附圖式，作 【實施方式】 組織細採光/件與照光方式來增進牙周 周組織錢，，可贿損傷的牙 的。 預防保養，_牙齒保健的目 此處所定⑽—至心崎細胞、牙㈣ 201208732 * 二:加:、、二周纖:維母細胞及牙周韌帶細胞。都可利用本發明 方法加以錢，如促料讀康。 w本發月 性，促進牙周組織細胞增生傾射’以增進牙周組織細胞活 足以保健的目的’本發明選用的能量範圍控制在 影響的照射能ΐ、::：二::避免 此里叩；丨於01焦耳/平方公分到10焦 量的選擇依據將於以下透過實驗數據加以說明。 =外’料二極體光源的發紐長，為因應 ，:以，量的差異，可有不同的最佳照射波 ^631^光^415±25奈米的藍光、575土25奈米的黃光、以 米的紅光波長範目尤佳。本發明發光二極體光源 的波長範圍亦可為數種不同波長光的組合，可對不同牙周組織 細胞產生不同減騎倾增生絲，因此可全綠促進牙齒 組織的保健。 在照光方式上，鑒於本發明在預防保健上的效果、便利性 實用性’本發明採取於適當的照射位置處同時照射一顆以上 牙齒的牙周組織細胞，可以使得多處牙周組織細胞同時活化， 以縮短照射_ ’提升使时的使用意願。關時間例如在 10秒鐘至20分鐘之間。 舉例而言，可使用牙套式的照光模組來達成此目的。任何 該領域中具有通常知識者，皆可在了解本發明的實施方式下， 採用各種不同的照光模組設計。本案於此處舉一實例，請參照 圖2所示，然此實例並不用以限制本發明方法的實施方式。 圖2所繪示的一種發光二極體照射裴置2〇〇，其包括本體 201208732 201、光照部202及電源供應單元203。在本實施例中，本體 201為U型結構，具有相對的内表面201a及外表面201b。内 表面201a及外表面201b之間.，具有一個連接部201c’用以和 内表面201a及外表面201b共同定義出一個牙齒容置槽201d; 可用來套置於使用者的上頜（或下頜）齒列（maxillary or mandibular teeth)。該本體的尺寸與材質設計，較佳可以讓使用 者的嘴唇在套置此裝置時仍可自然閉上，以方便曰常保健上的 頻繁使用。 光照部202 ’設置於本體201上，具有至少一個發光二極 體光源’例如複數個光源202a、202b、202c和202d，分別設 置於内表面201a及外表面201b。 發光二極體光源202a、202b、202c和202d的設置係於本 體套置於牙齒上時，位於牙齦及牙齒交界處附近，可使牙周組 織的照射達到較好的效果。由於光源所發射的光一般呈扇形發 散，例如可使用120度發散的光源。因此發光二極體光源 202a、202b、202c和202d與牙齒的距離，較佳可以使相鄰兩 光源所發射的光線有部分重疊，以使每顆牙齒的照光均勻。 發光二極體光源2〇2a、202b、202c和202d的選用功率雖 無特別限制，但應符合本發明的照射能量要求，即大約介於 0.1焦耳/平方公分到10焦耳/平方公分之間，以避免對牙齒、 牙周或其他口腔組織造成損傷。其中照射時間依據不同發光二 極體光源與照射能量要求，而有所差異。較佳的照射時間，: 約在10秒鐘至20分鐘之間，此照射時間亦適於日常保健之用。 另外，發光二極體光源202a、202b、202c和202d亦可依 實=翻時或部分開啟，以選擇性對牙齒及牙齦組織提供全 面*或區域性的光賴射，相增進牙周組織細胞活性的效 8 201208732 • j 果。 *r - 接著以上述方法對牙周組織細胞（包含齒槽骨細胞、牙齦 纖維母細胞、、牙周纖維母細胞及牙周㈣細胞)進行體外的(i η vitro)照射，並進行細胞活性試驗及細胞再生試驗來證實上 述方法確實具有促進牙顺織細胞錄及增進牙周組織細胞 再生的功效。試驗方法及結果詳述如下： . 一、細胞活性試驗： (1)細胞粒線體活性試驗 a以牙周細胞粒線體(mitochondria)活性表現及細胞鹼性磷 1酶(Alkaline Phosphatase，ALP)活性作為檢驗細胞活性的指 標。首先以依不同牙周組織細胞的生理特性，採用三種不同波 長的發光二極體光源(波長實質為652奈米的紅光發光二極體 光源、波長實質為590奈米的黃光發光二極體光源、和波長實 質為415奈米的藍光發光二極體光源），以及多種不同的照射 旎1(能量）’分別對含齒槽骨細胞、牙齦纖維母細胞、牙周纖 維母細胞及牙周韌帶細胞進行照射與培養。再利用MTT分析 法(MTT colorimetry assay)藉由 MTT 試劑 (3 4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide )被活 細胞粒線體(mitochondria)中琥珀酸脫氫梅(Succinatedehydrogenase) 的還原反應’分別量測含齒槽骨細胞、牙齦纖維母細胞、牙周 纖維母細胞及牙周韌帶細胞内粒線體的活性變化。實際試驗結 果請參照圖3A到圖3F : 圖3A係根據本發明的較佳實施例，所繪示的齒槽骨細胞 的粒線體活性試驗結果分析圖。橫軸代表不同的照射能量(〇 5 焦耳7平方公分、5焦耳/平方公分和10焦耳/平方公分）；縱軸 為齒槽骨細胞，經過三天培養後的活性比值，其係將所量測之 e 9 201208732 粒線體活性除以未照光的控制組的粒線體活性平均值。 根據量測結果，可觀察到，採用藍光發光二極體光源照射 齒槽骨細胞，在照射能量為〇.5焦耳/平方公分時，粒線體的活 性有最佳表現。若採用紅光或黃光發光二極體光源進行昭射\ 在照射能量為5焦、耳/平方公分時’有最佳表現。若與^照光 的控制組相較，可促進細胞活性將近2倍。S 4 201208732 (Gingival Fibroblast'GF), the luminous source of the light-emitting diode source is 415±25 nm, and the irradiation energy is between 1 Joules/cm 2 and 5 Joules/cm 2 . A knife In an embodiment of the invention, the periodontal tissue comprises gingival fibroblasts, and the illuminating diode light source has an illumination wavelength of 575±25 nm, and the irradiation energy is between 1 Joule/cm 2 and 10 Joules/square. Between the cents. In an embodiment of the invention, the periodontal tissue comprises gingival fibroblasts, and the illuminating one-pole light source has an illumination wavelength of 635±25 nm, and the irradiation energy is between 1 Joule/cm 2 and 10 Joules/cm 2 . between. In an embodiment of the invention, the periodontal tissue comprises periodontal fibroblast (PF), the illumination light of the light emitting diode source is 415±25 nm, and the irradiation energy is between 1 Joule/cm 2 . Up to 5 joules / square centimeter. In an embodiment of the invention, the periodontal tissue comprises periodontal fibroblasts, and the illumination wavelength of the illuminating one-pole source is 575±25 nm, and the irradiation energy is between 1 Joule/cm 2 and 10 Joules/cm 2 . between. In an embodiment of the invention, the periodontal tissue comprises periodontal fibroblasts, and the illumination wavelength of the illuminating one-pole source is 635 ± 25 nm, and the irradiation energy is between 1 Joules/cm 2 and 10 Joules/cm 2 . between. In an embodiment of the invention, the periodontal tissue comprises a periodontal ligament (PL) cell, the illumination light of the illuminating diode source is 415±25 nm, and the irradiation energy is between 〇2 joules/cm 2 . To $ joules per square centimeter. In an embodiment of the invention, the periodontal tissue comprises periodontal ligament cells, the illumination wavelength of the illuminating diode source is 575±25 nm, and the irradiation energy is between 〇.2 joules/cm 2 to 5 joules/square. Between the cents. 201208732 l In an embodiment of the invention, the periodontal tissue comprises periodontal ligament cells, and the illumination wavelength of the illuminating one-pole light source is 63 5±25 nm, and the irradiation energy is between 〇. 5 joules/square. Between centimeters and 6 joules per square centimeter. 9 In one embodiment of the invention, the plurality of illuminating diodes are used to illuminate the periodontal tissue of the plurality of teeth of the sigma region, and the adjacent two illuminates The illumination range of the light emitted by the diode source partially overlaps, so that the irradiation energy of the periodontal tissue of each tooth is between Joule/cm 2 and 6 Joules/cm 2 . The position of the illumination of the illuminating triode source is in the intersection of the tooth and the gum of the tooth. You illuminate the application, the tree _ proposes - (4) has a wavelength and weekly tissue cell activity. Because the tissue used in the sputum is irradiated to promote the lower teeth, it is similar to the direct light. The secret light-level body light source, the radiation energy is more suitable for lifting, periodontal or other oral tissues, and the prevention and maintenance of the teeth can reach the above-mentioned and other purposes of the present invention. According to the preferred embodiment, and in conjunction with the drawings, [Embodiment] The fine light/piece and illumination method are organized to promote the tissue of the periodontal week, and the damaged tooth can be bribed. Preventive maintenance, _ dental care purposes here (10) - to the heart of the cells, teeth (four) 201208732 * Two: plus:, two weeks of fiber: Victorian cells and periodontal ligament cells. All of the methods of the present invention can be used to make money, such as promoting consumption. w This hair is monthly, promotes the proliferation of periodontal tissue cells to enhance the activity of periodontal tissue cells for health care. The energy range selected by the present invention is controlled by the influence of irradiation energy. ::: 2:: Avoid this选择; The selection criteria for the range of 01 J/cm to 10 Coke will be explained below through experimental data. = The outer diameter of the 'dipole light source' is the corresponding, the difference between the quantity and the quantity, there may be different optimal illumination waves ^ 631 ^ light ^ 415 ± 25 nm blue light, 575 soil 25 nm The yellow light and the red light wavelength of the meter are especially good. The wavelength range of the light-emitting diode light source of the present invention can also be a combination of several different wavelengths of light, which can produce different kinds of reduced-rising proliferation filaments for different periodontal tissue cells, so that the whole green can promote the health care of the tooth tissue. In view of the illumination method, in view of the effect of the present invention on preventive health care, convenience and practicality, the present invention can simultaneously irradiate periodontal tissue cells of more than one tooth at an appropriate irradiation position, so that multiple periodontal tissue cells can be simultaneously Activate to shorten the exposure _ 'Improve the willingness to use. The off time is for example between 10 seconds and 20 minutes. For example, a braces-type illumination module can be used for this purpose. Anyone having ordinary knowledge in the art can use a variety of different illumination module designs in understanding the embodiments of the present invention. In this case, an example is given here, please refer to FIG. 2, but this example is not intended to limit the implementation of the method of the present invention. FIG. 2 illustrates a light-emitting diode illumination device 2 that includes a body 201208732 201, an illumination unit 202, and a power supply unit 203. In the present embodiment, the body 201 has a U-shaped structure with opposing inner and outer surfaces 201a and 201b. Between the inner surface 201a and the outer surface 201b, there is a connecting portion 201c' for defining a tooth receiving groove 201d together with the inner surface 201a and the outer surface 201b; and can be used for the user's upper jaw (or lower jaw) Maxillary or mandibular teeth. The size and material design of the body preferably allows the user's lips to naturally close when the device is placed, so as to facilitate frequent use of the health care. The illuminating portion 202' is disposed on the body 201, and has at least one illuminating diode light source ', for example, a plurality of light sources 202a, 202b, 202c, and 202d disposed on the inner surface 201a and the outer surface 201b, respectively. The arrangement of the light-emitting diode light sources 202a, 202b, 202c, and 202d is such that when the body is placed on the teeth, it is located near the junction of the gums and the teeth, so that the irradiation of the periodontal tissue can achieve a better effect. Since the light emitted by the light source is generally fan-shaped, for example, a 120-degree divergent light source can be used. Therefore, the distance between the LED light sources 202a, 202b, 202c and 202d and the teeth is preferably such that the light emitted by the adjacent two light sources partially overlaps to make the illumination of each tooth uniform. The power selection of the light-emitting diode light sources 2〇2a, 202b, 202c, and 202d is not particularly limited, but should meet the irradiation energy requirement of the present invention, that is, between about 0.1 Joules/cm 2 and 10 Joules/cm 2 . To avoid damage to teeth, periodontal or other oral tissues. The irradiation time varies according to the requirements of different light-emitting diode sources and irradiation energy. The preferred irradiation time is: between about 10 seconds and 20 minutes, and the irradiation time is also suitable for daily health care. In addition, the light-emitting diode light sources 202a, 202b, 202c, and 202d can also be turned over or partially turned on to selectively provide comprehensive* or regional light reflection on the teeth and gum tissue, and enhance the periodontal tissue cells. The effect of activity 8 201208732 • j fruit. *r - Following in vitro (i η vitro) irradiation of periodontal tissue cells (including alveolar bone cells, gingival fibroblasts, periodontal fibroblasts, and periodontal (four) cells), and cell viability Tests and cell regeneration assays have confirmed that the above methods do have the effect of promoting the recording of odontocytes and enhancing the regeneration of periodontal tissue cells. The test methods and results are detailed as follows: 1. Cell viability test: (1) Cell mitochondrial activity test a shows the activity of mitochondria and the alkaline alkaline phosphorus 1 (Alkaline Phosphatase, ALP). Activity is used as an indicator to test cell viability. Firstly, according to the physiological characteristics of different periodontal tissue cells, three different wavelengths of light-emitting diode light source (red light emitting diode light source with a wavelength of 652 nm and yellow light emitting diode with a wavelength of 590 nm) Body light source, and a blue light emitting diode source with a wavelength of 415 nm, and a variety of different illuminations 1 (energy)' for tooth-containing bone cells, gingival fibroblasts, periodontal fibroblasts, and teeth The ligamentum cells are irradiated and cultured. Dehydrogenated succinate in mitochondria by MTT assay using MTT colorimetry assay (3 4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide The reduction reaction of Succinate dehydrogenase] measured the activity of mitochondria in alveolar bone cells, gingival fibroblasts, periodontal fibroblasts and periodontal ligament cells, respectively. The actual test results are shown in Figs. 3A to 3F: Fig. 3A is a graph showing the results of the mitochondrial activity test of the alveolar bone cells according to a preferred embodiment of the present invention. The horizontal axis represents different irradiation energies (〇5 joules 7 cm ^ 2 , 5 joules / cm ^ 2 and 10 joules / cm ^ 2 ); the vertical axis is the alveolar bone cells, and the activity ratio after three days of culture is measured. Measured e 9 201208732 The mitochondrial activity was divided by the average of the mitochondrial activity of the unlit control group. According to the measurement results, it can be observed that the alveolar bone cells are irradiated by the blue light emitting diode light source, and the activity of the granular body is best when the irradiation energy is 焦5 joules/cm 2 . If the red or yellow light emitting diode source is used for the singularity, the best performance is obtained when the irradiation energy is 5 joules and the ear/square centimeter. Compared with the control group of illuminating light, it can promote cell activity nearly twice.
圖3B和圖3C根據本發明的較佳實施例，所分別繪示的牙 齦纖維母細胞粒線體活性試驗結果分析圖。其中，圖3b和圖 的牙齦纖㈣細胞，齡別叫㈤光照雜量 奶焦耳/付公分；狀為5焦硏方公分）。橫=圖表^ 照射後的培養天數；縱軸為代表細胞數量的吸输 W3B and 3C are graphs showing the results of the mitochondrial activity test of the gingival fibroblasts, respectively, in accordance with a preferred embodiment of the present invention. Among them, Figure 3b and Figure of the gingival fiber (four) cells, the age is not called (five) light amount of milk coke / pay centimeters; the shape is 5 coke square centimeters).横=图^ The number of days of culture after irradiation; the vertical axis is the absorption of the number of cells.
Density，OD )。 由1測結果可崎$，採用藍紐光二極體 纖維母細胞，在縣能量為2.5料/付公料有最佳= 見齦 右_紅光或黃光二極體光源進行縣，财照射能 隹 方公分時，粒線體活性會有最絲現。若與未照光的控制组 相較’可促進細胞活性近2倍。 J、、10· 圖3D和圖3E根據本發明的較佳實施例，所分 牙周纖維母細雜線體活輯驗結果分析圖。其巾犯如 =3E的牙齦纖維母細胞，係分別料同光照射能量進行照 L ^為2.5焦耳/平方公分；圖3E為5焦耳/平方公分)橫 照射後的培養天數;縱軸為活性比值，其係將所量測 ’立活性除以未照光的控制組的粒線體活性平均值。/ 由量測結果可以看出，採用藍 胞’在照射能量為2.5焦耳/平方公分時，粒= 會有最佳表現。若以用紅光或黃光發光二極體光源進行照 201208732 射’則照射能量為5焦、耳/平方公分時，粒線體活性會有最佳表 現。若與未照光的控制組相較，可促進細胞活性近2倍。 圖3F係根據本發明的較佳實施例，所繪示的牙周韌帶細 胞的粒線體活性試驗結果分析圖。橫軸代表不同實驗組的照 能量(0.5焦、耳/平方公分、2焦耳/平方公分和5焦耳/平方公 分）；縱軸為活性比值，其係將實驗組所量測之粒線體活性， 除以未照光的控制組的粒線體活性平均值。 根據量測結果’採用上述三種不同波長的發光二極體光源 照射後的牙周㈣細胞，經過三天培養後，可觀制光刺激對 牙周勤帶粒線體活性表現的影響。其中，牙肋帶細胞粒線體 ，活性表現。在採用藍光發光二極體光源進行照射時，照射能 量為〇.5焦耳/平方公分時，有最絲現。在制紅光與黃光 發光二極體光源進行照㈣，以照射能量^ 2纟耳/平方公分 時，有最佳表現。若與未照光的控制組相較，可促進 二 將近2倍。 $ ⑺細胞内鹼性磷酸性酶試驗（ALpase挪#， phosphatase assay) 依不同牙周!且織細胞的生理特性(phen〇type)，採用三種不 同波長的發光二極體統(波長實質為652奈米的紅光發光二 極，光源；皮長實質為’奈米的黃光發光二極體光源、和油 長實質為415奈米的藍光發光二極體光源），以及多種不同纪 照射能量，騎含齒射細胞、牙齦纖維母細胞、牙周纖绅 母細胞及牙職帶細胞騎騎與培養。再分別到含齒則 細胞、牙齦纖維母細胞、牙周纖維母細胞 性磷酸酶的活性變化。 由於細胞内的鹼性磷酸酶在特定環境下，能催^ 201208732 t p-nitrophmylatephosphate 反應；而所生成的 p_nitr〇phmylate anion在405nm有最大咴光波峰。故可藉由檢驗細胞内部的 p-mtrophrnylate anion得到鹼性磷酸酶的活性值。實際試驗結 果請參照圖4A到圖41 : 圖4A到圖4C係根據本發明的較佳實施例，所分別繪示 的齒槽骨細胞内鹼性磷酸性酶試驗的結果分析圖。試驗的分組 方式，係先採用，三種不同光源進行分組（圖4A到圖4(：分別 為波長實質為652奈米的紅光發光二極體光源、波長實質為 590奈米的页光發光二極體光源、和波長實質為4丨5奈米的藍 光發光一極體絲採用相同波長的實驗組中，又分別採用 0.1焦=/平方公分、0.5焦、耳/平方公分、5焦耳/平方公分和 焦耳平方公分四種不同照射能量為來對齒槽骨細胞進行照 射。經過培養後’進储射細胞崎㈣雜酶濃度(㈣細) 的量測其中’檢軸代表不同的培養天數；縱軸為齒槽骨細胞 内鹼性磷酸酶的濃度。 =—4A到圖4C可看出，不論採用藍光發光二極體光源、 1 ，體ί源或黃光發光二極體光源，對齒槽骨細胞進 行’都顯著地增進驗性磷酸性 =槽骨_，在照射能量為05焦耳,平方公二= 5隹耳/平誠黃結㈣射_，在蘭能量為 5焦耳/千方公分時，可達到最佳表現。 圖4D到圖4F係根據本發明的較 3 ίΐ 公分、2焦耳/平方公分和5焦耳/平方 種照射能量，來進行分組(圖4D到圖4F)。每一實驗組 刀丨以紅光發光二極體光源、黃光發光二極體光源、和藍光 201208732 發光二極體光源，對牙齦纖維母細胞進行照射。經過培養後， 進行牙齦纖維母細胞内鹼性磷酸性酶濃度的量測。 其中，橫軸代表不同的培養天數；縱軸為活性比值，其 將所量測之牙齦纖維母細胞内鹼性磷酸酶的濃度，除以未照光 的控制組之牙齦纖維母細胞内驗性填酸酶的濃度平均值。“ 由圖4D到圖4F可看出，不論採用藍光發光二極體光源、 =光，光二極體光源或黃光發光二極體絲，對牙齦纖維母細 匕進行照射，都促進細胞分泌(secret)鹼性磷酸晦。當昭射能量 0.5焦耳坪方公分時，_藍絲對於牙賴科細胞進#照 于一了達到最佳表現，當照射能量為2焦耳/平方公分時，採用 紅，來對於牙齦纖維母細胞進行照射，可達到最佳表現；當照 射能量為5焦耳/平方公分時，採用黃光來對於牙酿纖維母細 進行照射’可達到最佳表現。 圖4G到圖41係根據本發明的較佳實施例，所分別繪示的 牙周纖維母細胞内鹼性磷酸性酶試驗的結果分 0-5 分二種照射能量，來進行分組(4G到圖41)。每一實驗組再分 別以紅光發光二極體絲、黃光發光二極體光源、和藍光發光 二極體光源，對牙周纖維母細胞進行照射。經過培養後，進行 牙周纖維母細胞内鹼性磷酸性酶濃度的量測。 ，中，橫軸代表不同的培養天數；縱軸為活性比值，其係 將所量測之牙齦纖維母細胞内鹼性磷酸酶的濃度，除以未照光 的控制組之牙齦纖維母細胞内驗性鱗酸酶的濃度平均值。 ，由圖4G到圖41可看出，不論採用藍光發光二極體光源、 紅光發光二極體光源或黃光發光二極體光源，對牙周纖維母細 胞進行照射，都促進細胞分泌鹼性磷酸晦。尤其是藍光發光二極 201208732 體光源在照射能量實質為0.5焦耳/平方公分；紅光及黃光發光 二極體光源在照射能量實質為5焦耳/平方公分時，細胞活性可 達到最佳表現。若與未照光的控制組相較，可促進細胞鹼性磷 酸晦分泌近2倍。 二、細胞再生試驗 以照光後細胞增生的數量，作為增進細胞再生的指標^首、 先以依不同牙周組織細胞的生理特性，採用三種不同波長的發 光二極體光源(波長實質為652nm的紅光發光二極體光源、波 長實質為590nm的黃光發光二極體光源、和波長實質為415nm 的藍光發光二極體光源）’以及多種不同的照射能量’分別對 含齒槽骨細胞、牙齦纖維母細胞、牙周纖維母細胞及牙周韌帶 細胞進行照射與培養。再分別利用細胞計數器，量測含齒槽骨 細胞、牙齦纖維母細胞、牙周纖維母細胞及牙周韌帶細胞内粒 線體的細胞增生密度。實際試驗結果請參照圖5A到圖5F : 圖5A係根據本發明的較佳實施例，所繪示的齒槽骨細胞 照光後細胞增生試驗的結果分析圖。橫軸代表不同的照射能量 (分別為0.5焦耳/付公分、5焦耳坪方公分和10焦耳/平方 公分）；縱軸為齒槽骨細胞密度比值，其為光照實驗組齒槽骨 細胞數除2未照光的控制組之齒槽骨細胞數平均值。 根據量測結果’可觀察到，採用藍光發光二極體光源照射 齒槽月細胞’在照射能量為〇 5焦耳/平方公分時，對齒槽骨細 胞的增生有最佳的促進作用；若採用紅光與黃光發光二極艘光 源進，照射’财照賴量為5料/平方公糾，有最佳表 現右與未照光的控制組相較，可促進齒槽骨細胞數量增生高 達1.5倍》 圖5B寺圖5C根據本發明的較佳實施例，所分別繪示的 201208732 r 牙齦纖維母細胞照光後細胞增生試驗結果分析圖。其中，圖 5B和圖5C的牙齦纖維母細胞係分別以不同光照射能量進行 =射（圖5B為〇.5焦耳/平方公分；圖5C為5焦耳/平方公分）。 秩軸代表光照射後的培養天數；縱軸為牙齦纖維母細胞密度比 值’其為光照實驗組牙齦纖維母細胞數除以未照光的控制組之 牙齦纖維母細胞數平均值。 根據量測結果，可觀察到，採用藍光發光二極體光源照牙 齦纖維母細胞，在歸能量為〇·5 f、耳坪方公糾，對牙銀纖 維母細胞的增生有最佳的促進作帛；若採用红光與冑光發光二 極體光源進行· ’财照·量為5料/平方公分時有最 =表現。若與未照光的控制組相較，可促進牙齦纖維母細胞 量增生將近1.4倍。 圖5D和圖5E根據本發明的較佳實施例，所分別繪示的 周，維母細胞照光後細胞增生(pr〇liferati〇n)試驗結果分析 昭。”中，圖5D和圖5E的牙齦纖維母細胞係分別以不同光 ^能量進行照射(圖5D為0.5焦耳/平方公分；圖5£為5焦 維母橫轴代表光照射後的培養天數；縱軸為牙周纖 Γ又比值’其為光照實驗組牙周纖維母細胞數除以未 …、九的控制組之牙周纖維母細胞數平均值。 牙周果’可觀察到’採賴光發光二極體光源照射 。義、’隹母、、’田胞’在照射能量為〇 增=最佳的，進作用;若採用心與= 射，則在騎能量為5焦耳/平方公分時有 數量增光的控制組相較’可促進牙周纖維母細胞 圖F係根據本發明的較佳實施例，所繪示的牙周拿刃帶細 a 15 201208732 胞照光後細胞增生試驗的結果分析圖。橫軸代表不同的照射能 量(分別為0.5焦耳/平方公分、5焦耳/平方公分和1〇焦耳/平 方公分），縱軸為牙周韌帶細胞密度比值，其為光照實驗組牙 周韌帶細胞數除以未照光的控制組之牙周韌帶細胞數平均值。 根據量測結果，可觀察到，採用藍光發光二極體光源照射 牙周韌帶細胞，在照射能量為〇.5焦耳/平方公分時，對牙周韌 帶細胞的增生有最佳的促進作用；若採用紅光與黃光發光二極 體光源預射’财照舰量為5焦耳/付公分時有最佳 表現。若與未照光的控制組相較，可促進牙周韌帶細胞數量增 生將近1.7倍。 、-.'T、上所述，根據買測結果，採用本發明之實施例所提供的 方法及裝置，對牙周組織細胞進行照射，確實可促進牙周組織 細胞活性提高’促進細胞分化，並進_激牙周組織細胞增生。 而且，當採用藍光發光二極體光源照射牙周組織細胞時， /、增進齒槽骨細胞活性，並促進細胞增生的較佳照射能量，實 料/平方公分到5焦耳/平方公分U最佳照 仓月匕1為。·5焦耳/平方公分。增進牙齦纖維母細胞活性，並促 進細胞増生的較佳照雜量’實質介於1隹耳/平方公分们 分之間；且最賴職量為2.5斜7平方公分。 周纖維母細胞活性’並促進細胞增生的較佳照射能量， 射能=if耳/平方公分到5焦耳/平方公分之間；且最佳照 細胎；.焦、耳/平方公分。增進牙周勤帶細胞活性，並促進 隹胞s生的難照龍量’實質介於（U f、耳/平方公分到5 …耳=方公分U最佳照龍4為G 5焦耳/平方公分。 當^黃光和紅光發光二極體光源進行照㈣，其增進齒 僧月，雜，並促進細胞增线有朗射能量，實質介於i 201208732 焦耳/平方公分到10焦耳/平方公分之間；且最佳照射能量為5 焦耳/平方公分。增進牙齦纖維母細胞活性，並促進細胞增生 的有效照射能量，實質介於丨焦耳/平方公分到10焦耳/平方公 分之間；且最佳照射能量為5焦耳/年方公分。增進牙周纖維 母細胞活性，並促進細胞增生的有效照射能量，實質介於1焦 耳/平方公分到10焦耳/平方公分之間；且最佳照射能量為.5 焦耳/平方公分。增進牙周韌帶細胞活性，並促進細胞增生的 有效照射能量，實質介於0.5焦耳/平方公分到6焦耳/平方公 分之間；且最佳照射能量為2焦耳/平方公分。 值得一提的是，根據試驗顯示，由於本發明之實施例係採 用低功率發光二極體進行照射，光照能量所造成的溫度變化， 皆在細胞可容許的範圍内。因此，在提高細胞活性，刺激組織 細胞再生的同時，並不會產生明顯的熱效應而影響細胞的正常 生理表現。 另外，藉由分析照光後牙周細胞(齒槽骨細胞、牙齦纖維 母細胞、牙周纖維母細胞及牙周韌帶細胞）的細胞程式凋亡 (apoptosis)結果，更可發現，照射能量超過1〇焦耳/平方公分 以上，方有可能對牙周細胞造成明顯傷害。更顯示採用本發日^ 之實施例所提供的方法及裝置，並不會造成牙齒、牙周或^他 口腔組織造成損傷。具有安全性佳’操作簡便的技術優勢7適 合提供一般人日常牙齒預防保養。 根據上述實施例，本發明係提出一種使用具有特定波長與 照射能量之發光二極體光源，對牙周組織進行照射，以增進^ 周組織細胞活性，促進牙周組織細胞增生。由於所採用=發光 二極體光源，輻射能量較低，直接照射並不會對不會對牙^、 牙周或其他口腔組織造成損傷，適合提供一般人日^牙齒預防 17 201208732 保養，可達到保健牙齒組織的目的。 雖然本發明已以較佳實施例揭露如上，然其並非用以限定 本發明’任何熟習牝技藝者，在不脫離本發明之精神和範圍 内’當可作些許之更動與潤飾，因此本發明之保護範圍當視後 附之申請專利範圍所界定者為準。 【圖式簡單說明】 圖1係繪示一人類牙齒及牙周組織的結構剖面圖。 圖2係根據本發明一實施例，所繪示的一種發光二極體照 射裝置的結構示意圖。 圖3A到圖3F係根據本發明的較佳實施例，所繪示的一 系列牙周組織細胞的粒線體活性試驗結果分析圖。 4A到圖41係根據本發明的較佳實施例，所繪示的齒槽一 系列牙周組織細胞内鹼性磷酸性酶試驗的結果分析圖。、 圖5A到圖5F係根據本發明的較佳實施例，所繪示的一 系列牙周組織照光後細胞增生試驗的結果分析圖。 【主要元件符號說明】 12 :牙周組織 122 :齒槽骨 124 :及牙骨質 201 :本體 201b :本體外表面 201d :牙齒容置槽 202a :光源 202c :光源 1〇 :牙齒 121 :牙周韌帶 123 I牙齦 20〇 :發光二極體照射裝置 201a.本體内表面 201c :連接部 202 :光照部 202b .光源 201208732 202d :光源 203 :電源供應單元Density, OD). From the 1 test result, you can use the blue neon diode fibroblast, and the energy in the county is 2.5 material/paid material. The best = see 龈 right _ red light or yellow light diode light source for county, financial illumination When the square is divided, the mitochondrial activity will be the most vivid. If compared with the unlit control group, it can promote cell activity nearly 2 times. J, 10, Fig. 3D and Fig. 3E are diagrams showing the results of the test of the periodontal fiber matrix fine-chain body in accordance with a preferred embodiment of the present invention. The gingival fibroblasts such as =3E are treated with the same light irradiation energy as L ^ is 2.5 joules / cm ^ 2; Figure 3E is 5 joules / cm ^ 2) the number of culture days after horizontal irradiation; the vertical axis is active The ratio is the average activity of the mitochondrial activity of the control group divided by the measured activity. / It can be seen from the measurement results that the granule = will have the best performance when the irradiation energy is 2.5 joules/cm 2 . The mitochondrial activity is best exhibited when the irradiation energy is 5 joules or ears per square centimeter with a red or yellow light emitting diode source. Compared with the unilluminated control group, it can promote cell activity nearly 2 times. Fig. 3F is a graph showing the results of a test for the activity of the mitochondrial activity of the periodontal ligament cells according to a preferred embodiment of the present invention. The horizontal axis represents the illuminating energy of different experimental groups (0.5 joules, ears/cm ^ 2 , 2 joules / cm ^ 2 and 5 joules / cm ^ 2 ); the vertical axis is the activity ratio, which is the mitochondrial activity measured by the experimental group. , divided by the average of the mitochondrial activity of the unlit control group. According to the measurement results, the periodontal (four) cells irradiated by the above three different wavelengths of the light-emitting diode source were used, and after three days of cultivation, the effect of the light stimulation on the activity of the periodontal mitochondria was observed. Among them, the ribs have cell mitochondria and are active. When irradiated with a blue light-emitting diode source, the irradiation energy is 〇5 joules/cm 2 , which is the most vivid. In the red and yellow light-emitting diode source (4), the best performance is obtained when the energy is 2 2 ears/cm 2 . If compared with the unlit control group, it can promote nearly two times. $ (7) Intracellular alkaline phosphatase assay (ALpase #, phosphatase assay) Depending on the periodontal and ph 〇 physiologic properties (phen 〇 type), three different wavelengths of luminescent diodes are used (the wavelength is 652 nm) The red light emitting diode of the meter, the light source; the skin length is 'nine's yellow light emitting diode light source, and the oil length is 415 nm blue light emitting diode light source), and a variety of different irradiation energies, Riding and culturing with dentate cells, gingival fibroblasts, periodontal fibroblasts, and dental cells. Then, the activity of the cells, the gingival fibroblasts, and the periodontal fibroblast phosphatase were changed. Because the alkaline phosphatase in the cell can respond to the 201208732 t p-nitrophmylatephosphate reaction in a specific environment, the generated p_nitr〇phmylate anion has the largest calender peak at 405 nm. Therefore, the activity value of alkaline phosphatase can be obtained by examining the p-mtrophrnylate anion inside the cell. The actual test results are shown in Figs. 4A to 41: Fig. 4A to Fig. 4C are diagrams showing the results of the results of the alkaline alkaline phosphorylase test in the alveolar bone according to a preferred embodiment of the present invention. The grouping method of the experiment is firstly adopted, and three different light sources are grouped (Fig. 4A to Fig. 4 (: respectively, a red light emitting diode light source with a wavelength of substantially 652 nm, and a page light emitting light with a wavelength of substantially 590 nm) The polar body light source and the blue light emitting monopole body having a wavelength of substantially 4 丨 5 nm use the same wavelength in the experimental group, and respectively adopt 0.1 jog=/cm 2 , 0.5 joule, ear/cm 2 , 5 joule/square. The alveolar bone cells are irradiated by four different irradiation energies of centimeters and joules square centimeters. After the culture, the concentration of the microcapsules (four) of the storage cells is measured, and the 'axis is the number of different culture days; The vertical axis is the concentration of alkaline phosphatase in the alveolar bone cells. From -4A to Fig. 4C, it can be seen that whether the blue light emitting diode source, the 1, the source or the yellow light emitting diode source, the tooth The trough cells perform 'all significant enhancement of the phosphoric acid = trough _, the irradiation energy is 05 joules, the square metric = 5 隹 ear / Ping Cheng Huang knot (four) shot _, the energy in the blue energy is 5 joules / thousand square At the time of centimeters, the best performance can be achieved. Figures 4D to 4F are based on Invented more than 3 ΐ cm, 2 joules / cm ^ 2 and 5 joules / square of irradiation energy to group (Figure 4D to Figure 4F). Each experimental group of knives with red light emitting diode light source, yellow light The diode light source and the blue light 201208732 light-emitting diode light source irradiate the gingival fibroblasts. After the culture, the concentration of alkaline phosphorylase in the gingival fibroblasts is measured. Among them, the horizontal axis represents different cultures. The number of days; the vertical axis is the activity ratio, which is the average of the concentration of the alkaline phosphatase in the gingival fibroblasts measured by the illuminating fibroblasts in the unlit control group. It can be seen from Fig. 4D to Fig. 4F that whether the blue light emitting diode light source, the light, the light diode light source or the yellow light emitting diode wire is used to irradiate the gingival fiber fine sputum, the cell secretion is promoted (secret). Alkaline strontium phosphate. When the radiance energy is 0.5 joules per square centimeter, _ blue silk is used for the best performance of the ray ray cells. When the irradiation energy is 2 joules/cm 2 , the red color is used. Come to the teeth When the fibroblasts are irradiated, the best performance can be achieved; when the irradiation energy is 5 joules/cm<2>, the yellow light is used to irradiate the dendritic fiber matrix to achieve the best performance. FIG. 4G to FIG. 41 are diagrams according to the present invention. In the preferred embodiment, the results of the alkaline phosphorylase test in the periodontal fibroblasts, respectively, are divided into 0-5 points and the two irradiation energies are grouped (4G to Fig. 41). The periodontal fibroblast is irradiated with a red light emitting diode wire, a yellow light emitting diode light source, and a blue light emitting diode light source. After the culture, the alkaline phosphorylase in the periodontal fibroblast is performed. The concentration is measured. The middle axis represents the different culture days; the vertical axis represents the activity ratio, which is the concentration of the alkaline phosphatase in the measured gingival fibroblasts divided by the unlit control group. The mean concentration of endogenous luciferase in fibroblasts. It can be seen from Fig. 4G to Fig. 41 that the irradiation of periodontal fibroblasts, whether using a blue light emitting diode light source, a red light emitting diode light source or a yellow light emitting diode light source, promotes the secretion of alkali by the cells. Barium phosphate. In particular, the blue light emitting diode 201208732 body light source is substantially 0.5 joules per square centimeter of illumination energy; the red and yellow light emitting diodes achieve optimal performance when the irradiation energy is substantially 5 joules per square centimeter. Compared with the unilluminated control group, the cell alkaline bismuth phosphate can be promoted nearly twice as much. Second, the cell regeneration test to increase the number of cell proliferation after illumination, as an indicator of cell regeneration, first, according to the physiological characteristics of different periodontal tissue cells, using three different wavelengths of light-emitting diode light source (wavelength is 652nm a red light emitting diode light source, a yellow light emitting diode light source having a wavelength of substantially 590 nm, and a blue light emitting diode light source having a wavelength of substantially 415 nm) and a plurality of different irradiation energies respectively for the tooth-containing bone cells, Gingival fibroblasts, periodontal fibroblasts, and periodontal ligament cells were irradiated and cultured. Then, using a cell counter, the cell proliferation density of the granules in the alveolar bone cells, the gingival fibroblasts, the periodontal fibroblasts, and the periodontal ligament cells was measured. The actual test results are shown in Figs. 5A to 5F: Fig. 5A is a graph showing the results of a cell proliferation test after alveolar bone cell irradiation according to a preferred embodiment of the present invention. The horizontal axis represents different irradiation energies (0.5 joules per centimeter, 5 joules per square centimeter, and 10 joules per square centimeter, respectively); the vertical axis is the alveolar bone cell density ratio, which is the number of alveolar bone cells in the light experimental group. 2 The average number of alveolar bone cells in the unlit control group. According to the measurement results, it can be observed that the irradiation of the alveolar cells with the blue light emitting diode light source has the best promoting effect on the proliferation of alveolar bone cells when the irradiation energy is 〇5 joules/cm 2 ; Red light and yellow light emitting two-pole light source, the radiation 'quantity depends on 5 materials / square public correction, the best performance of the right and unilluminated control group, can promote the number of alveolar bone cell proliferation up to 1.5 Figure 5B is a diagram showing the results of cell proliferation test of 201208732 r gingival fibroblasts according to a preferred embodiment of the present invention. Here, the gingival fibroblast cell lines of Fig. 5B and Fig. 5C were respectively irradiated with different light irradiation energies (Fig. 5B is 〇5 joules/cm 2 ; Fig. 5C is 5 joules/cm 2 ). The rank axis represents the number of days of culture after light irradiation; the vertical axis represents the ratio of the density of gingival fibroblasts, which is the average of the number of gingival fibroblasts in the light-tested experimental group divided by the number of gingival fibroblasts in the unlit control group. According to the measurement results, it can be observed that the gingival fibroblasts are illuminated by the blue light emitting diode source, and the energy is 〇·5 f and the ear pings are corrected, which promotes the proliferation of silver fibroblasts. For 帛; if using red light and neon light source light source · 'Finance · quantity is 5 material / square centimeter when there is the most = performance. Compared with the unilluminated control group, it can promote the proliferation of gingival fibroblasts by nearly 1.4 times. Fig. 5D and Fig. 5E show, in accordance with a preferred embodiment of the present invention, the results of the test of cell proliferation after fluorescing cells in the pr〇liferati〇n. In the gingival fibroblast cell lines of Fig. 5D and Fig. 5E, respectively, irradiation with different light energy (Fig. 5D is 0.5 Joules/cm 2 ; Fig. 5 £ 5 is the focal length of the 5 Jiaowei mother representing the number of culture days after light irradiation; The vertical axis is the periodontal fibrosis and the ratio is 'the number of periodontal fibroblasts in the light-tested experimental group divided by the number of periodontal fibroblasts in the control group of No. and Nine. The periodontal fruit can be observed. Illumination of the light-emitting diode source. The meaning of the 'Yimu, 'Tianji' is the best in the irradiation energy = the best effect; if the heart and the = shot are used, the riding energy is 5 J/cm 2 When there is a quantity of light-increasing control group compared to 'can promote periodontal fibroblasts picture F', according to a preferred embodiment of the present invention, the periodontal leading edge band is a 15 201208732. Analysis graph. The horizontal axis represents different irradiation energies (0.5 joules/cm ^ 2 , 5 joules / cm ^ 2 and 1 〇 joules / cm ^ 2 , respectively), and the vertical axis is the ratio of periodontal ligament cell density, which is the periodontal of the light experimental group. The number of ligament cells divided by the unilluminated control group of periodontal toughness The average number of cells. According to the measurement results, it can be observed that the periodontal ligament cells are irradiated by the blue light emitting diode light source, and the proliferation of the periodontal ligament cells is optimal when the irradiation energy is 焦5 joules/cm 2 . The promotion effect; if the red light and the yellow light emitting diode source are used to pre-shoot the 'fair ship volume is 5 joules / centimeters, the best performance. If compared with the unilluminated control group, can promote the periodontal ligament The number of cells proliferated nearly 1.7 times. -, 'T, above, according to the test results, using the method and device provided by the embodiments of the present invention to irradiate the periodontal tissue cells, indeed promote the periodontal tissue cells Increased activity 'promotes cell differentiation, and stimulates periodontal tissue cell proliferation. Moreover, when irradiating periodontal tissue cells with blue light-emitting diode source, /, enhances alveolar bone cell activity, and promotes better irradiation of cell proliferation Energy, material / square centimeter to 5 joules / square centimeter U best photo period month 匕 1 · 5 joules / square centimeter. Improve the activity of gingival fibroblasts, and promote the better dose of cell growth The substance is between 1 隹/m2; and the most vacant position is 2.5 7 7 cm ^ 2. The preferred energy of irradiation of the fibroblasts and promoting cell proliferation, the energy of the shot = if the ear / square centimeter Between 5 joules / square centimeter; and the best care of fine tires; coke, ear / square centimeter. Improve the activity of the periodontal zone with cell viability, and promote the number of difficult cells of the scorpion s 'substance' (U f , ear / square centimeters to 5 ... ears = square centimeters U best according to the dragon 4 is G 5 joules / square centimeter. When ^ yellow light and red light emitting diode light source to take photos (four), which promotes the gums, miscellaneous, And promote the cell line to have a radiant energy, the essence is between i 201208732 joules / square centimeters to 10 joules / square centimeter; and the optimal irradiation energy is 5 joules / cm ^ 2. Improve the activity of gingival fibroblasts and promote cells The effective irradiation energy of hyperplasia is substantially between 丨 joules per square centimeter to 10 joules per square centimeter; and the optimal irradiation energy is 5 joules per year centimeters. The effective irradiation energy for promoting the activity of periodontal fibroblasts and promoting cell proliferation is substantially between 1 joule/cm 2 and 10 joules/cm 2 ; and the optimal irradiation energy is .5 joules/cm 2 . The effective irradiation energy for promoting the activity of periodontal ligament cells and promoting cell proliferation is substantially between 0.5 Joules/cm 2 and 6 Joules/cm 2 ; and the optimal irradiation energy is 2 Joules/cm 2 . It is worth mentioning that, according to tests, since the embodiment of the present invention is irradiated with a low-power light-emitting diode, the temperature change caused by the illumination energy is within the allowable range of the cells. Therefore, while increasing cell activity and stimulating tissue cell regeneration, it does not produce significant thermal effects and affects the normal physiological performance of the cells. In addition, by analyzing the results of apoptosis of periodontal cells (alveolar bone cells, gingival fibroblasts, periodontal fibroblasts, and periodontal ligament cells) after illumination, it was found that the irradiation energy exceeded 1 More than 〇 joules per square centimeter may cause significant damage to periodontal cells. It is further shown that the method and apparatus provided by the embodiment of the present invention do not cause damage to teeth, periodontal or other oral tissues. It has a good safety. The technical advantage of easy operation is suitable for providing daily dental care and maintenance for the average person. According to the above embodiment, the present invention proposes to irradiate periodontal tissue with a light-emitting diode light source having a specific wavelength and irradiation energy to enhance cell tissue activity and promote periodontal tissue cell proliferation. Due to the use of the light-emitting diode source, the radiant energy is low, and direct irradiation does not cause damage to the teeth, periodontal or other oral tissues. It is suitable for providing general human teeth and teeth prevention 17 201208732 Maintenance, which can reach health care The purpose of dental tissue. Although the present invention has been disclosed in the above preferred embodiments, the present invention is not intended to limit the invention, and the invention may be modified and modified without departing from the spirit and scope of the invention. The scope of protection is subject to the definition of the scope of the patent application. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a cross-sectional view showing the structure of a human tooth and periodontal tissue. 2 is a schematic structural view of a light emitting diode illumination device according to an embodiment of the invention. 3A through 3F are graphs showing the results of a mitochondrial activity test of a series of periodontal tissue cells according to a preferred embodiment of the present invention. 4A to 41 are graphs showing the results of a test of intracellular alkaline phosphorylase in a series of periodontal tissues of the alveolar according to a preferred embodiment of the present invention. 5A to 5F are diagrams showing the results of a series of periodontal tissue cell proliferation test according to a preferred embodiment of the present invention. [Main component symbol description] 12: Periodontal tissue 122: Alveolar bone 124: and cementum 201: Body 201b: External body surface 201d: Dental receiving groove 202a: Light source 202c: Light source 1〇: Teeth 121: Periodontal ligament 123 I gingival 20 〇: illuminating diode illuminating device 201a. body inner surface 201c: connecting portion 202: illuminating portion 202b. light source 201208732 202d: light source 203: power supply unit
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