TW201137124A - Immunomodulatory compositions - Google Patents

Abstract

The present invention relates to an immunomodulatory composition comprising a yolk product from eggs laid by female poultry which has been immunized with Helicobacter pylori. The immunomodulatory composition of the invention is effective in modulating an immune response in a subject. In particular, the immunomodulatory composition can promote production of IFN- γ and inhibit production of IL-4 and IL-5 in a subject.

Description

201137124 VI. Description of the Invention: [Technical Field] The present invention relates to an immunomodulatory composition which enhances an individual's immune response against a pathogen and reduces an inflammatory response. ~ [Prior Art] The immune system is a defense system used by the body to protect against foreign microorganisms, toxins, cancer cells of its own, or potentially harmful substances such as blood or tissues from others. The immune system can be divided into congenital and acquired immune systems. The innate immune system (also known as the non-specific immune system) serves as the first defense mechanism that provides immediate protection. The relevant members include natural killer cells, macrophages, neutrophils, and eosinophils. The acquired immune system (also known as the specific immune system) is the second defense mechanism that takes time to produce an immune response against a specific antigen, including T cells and B cells. The acquired immune system produces a helper T cell type 1 (Thl) immune response and a helper T cell type 2 (Th2) immune response. The Thl immune response mainly refers to the cell-mediated immune response, which involves the secretion of interleukin-2 (IL-2) and interferon-γ (IFN-γ) and the activation of cytotoxic T lymphocytes (CTLs). Fight against viral infections and cancer cells. The Th2 immune response mainly refers to the humoral immune response, which involves the secretion of interleukin-4 (IL-4) and interleukin-5 (IL-5) and the activation of B cells (antibody production) against free bacteria. Or parasites. It is generally believed that 'excessive secretion of IL-4 and IL-5 is associated with allergic and inflammatory responses. In addition, the provision of immunity can be achieved by active or passive immunization. Active immunity means that the body's immune system is activated after being stimulated by foreign substances (for example, germs), and actively generates immunity against the foreign substance. In contrast, passive immunization refers to the introduction of immune molecules (usually antibodies) against specific pathogens into the body. The body does not actively produce such immune molecules. In general, active immunity takes a long time to produce immunity, but the effect is long-lasting, while passive immunity can provide immunity faster than 201137124, but the effect is not lasting. The prior art has proposed a composition as an immunostimulating agent, and a plurality of Chinese herbal medicines can increase the secretion of U, but rises 6,464,982. 〇^ In addition, it is known that female birds are produced by specific pathogens. The egg yolk portion of the egg has a specific antibody against the specific condition, and the specific antibody required for isolation is isolated from the disease by passive immunization against the pathogen. Related art has been developed for many years and can be used, for example, to relieve diarrhea caused by Salmonella pmen.ow 59: 416-20), round 138: 143-8), and to shorten its abdominal wetness period. Specialized people can not use Helicobacter pylori (price) (7) to immunize hens, and then isolate the anti-Helicobacter pylori antibody from the yolk part of the maternal charm, which can be used to treat Helicobacter pylori infection ( flW Dzag Teng "c Qing ^; y / face _ / diaphragm muscle 9 (5): 1061-1066).

Mar to dt et al. (US 7,355, 〇92) discloses a genetic vaccine for providing E. coli cilia antigens, and demonstrates that after the genomic vaccine is administered to hens, the laid eggs can be collected and further isolated from the eggs to fight against ciliated antigens. The antibody provides passive immunity against diarrheal diseases in humans or animals. However, there is no literature to disclose the effect of a female poultry egg produced by antigen stimulation to regulate the immune function of an individual. SUMMARY OF THE INVENTION The present invention discloses for the first time that an egg yolk product derived from eggs produced by Helicobacter pylori-immunized female birds has the immune function of t-week individuals, in particular, can enhance natural killer cell toxicity and promote IFN_Yi production. Inhibition of IL-4 and IL-5 production, thus allowing the individual's immune response to Thl immune response 'to avoid the occurrence of Th2 inflammatory response, help to combat foreign pathogens and reduce inflammatory response. Thus, in one aspect, the invention provides an immunomodulatory composition comprising 201137124 duck, or goose.杈佳者' The female poultry is chicken, yellow and ==== epidemic; = step-by-step includes the weight ratio of mulberry yellow product, mulberry yellow and chitosan vinegar is the egg contained in the composition. Typically, the immunological composition of the present invention can be used as an i-additive or a detailed description of the specific examples in the specific examples of the present invention, and the following description is intended to limit the rest. The disclosure of the content. Use instead of any side [Embodiment] In the aspect of the present invention, an immunomodulatory composition is provided: a: abbreviated|the main yellow product is derived from a Helicobacter pylori, a female bird thereof egg. It’s only 4, and it’s a singularity. All the technical and scientific techniques used here are as good as those of the ordinary technology in the art of the present invention. "B", if not specified, ^ refers to the number of one (one or more). Qualitative time immunization - g 卩 "B" "describes" - the reagent or substance has changed or adjusted at least - the immune system However, it is not limited to 'changing or adjusting the number (or content/activity) of member cells or knives (eg, cytokines and antibodies) of the immune system. Various test methods have been developed in the related art to evaluate a reagent or substance. The immune regulation function, such as 'the evaluation method of healthy immune regulation Wei published by the Department of Health of the Executive Yuan', including the analysis of the activity of the gambling hand and the cell stimulating 201137124, the yolk product used in the details "B" means - isolated from the egg yolk miscellaneous, which can be unprocessed or added = production mode 'including but not limited to, freeze-drying. By "using a reagent or substance", the immune response of the individual is activated, preferably, to produce a specific immune response against the agent or substance. The shoulder body is capable of administering H. pylori according to the present invention. To the sex poultry epidemic ^ 'Ran Wei Jing and other female poultry eggs 5 to avoid cold), and then get 1 yellow = yolk portion of the egg produced by the worm, can be isolated from the snail and the special antibody In order to treat H. pylori II, the Ming system first revealed and confirmed that the egg yolk product obtained from the ff of Helicobacter pylori-immunized S has a _ _ effect. As shown below, Ϊ No, Ϊ compared with the control group, taking The mice with these egg yolk products face the challenge of ΐ ΐ , , , , , 展现 增强 增强 增强 增强 增强 增强 IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN IFN Helicobacter pylori, which is vaccinated against female cockroaches by the quotient of ^ ί ί 可由 适 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Product. Take appropriate = Spirulina solution The cell product is mixed with an adjuvant, and the plague effect is administered by intramuscular injection. After an appropriate time, the embryonic poultry hair is collected: the yellow part is further obtained to obtain the egg yolk product. Complicated poultry, including buttocks, ducks and cranes, 201137124 is preferably chicken. The above-mentioned technology for immunizing female birds with Helicobacter pylori and the subsequent acquisition of the yolk fraction has been done for this. ^ (^) 〇 加 加 骑 骑 加 加 可 可 可 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加 加In the mulberry mulberry, the immunomodulatory composition of the present invention can be further processed into a sapling, and the perennial, Phytophthora fungi, which is parasitic on mulberry trees, is a traditional Chinese medicinal material and is commercially available. In recent years ί °»f=九报口 ^ 桑 桑 具有 has anti-oxidation, anti-mutation, anti-tumor activity, and protect the liver _. It is quite popular to use the chitin to remove some or all of the products of the brewing base. In addition to this, the activity of lipids and the activities of enhancing immunity, anti-tumor and anti-caries are the most widely studied. In a specific embodiment, the immunomodulatory composition of the present invention comprises * to the vaccinated female Helicobacter pylori, a fragment thereof or a combination of ί ΐ ί: ίΐ: ί eggs, and mulberry yellow and chitosan Sugar, wherein the weight ratio of the egg re-product, mulberry to chitosan is about 2:2:1. Use, ΐ ϊ ϊ ϊ 为 为 为 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县 县. Immunity suitable for use in the present invention ^ 201137124 = Ingredients include, but are not limited to, diluents, binders, lubricants, antioxidants, colorants, fillers, preservatives, and stabilizers. Useful diluents include sodium carboxymethylcellulose, sorbitol, talc, dextran, lactose, sucrose, mannitol, and the like. Useful binders include gum arabic, sodium alginate, ethyl cellulose, seaweed, gelatin, starch, hydroxycellulose, hydroxypropylcellulose, and the like. Useful lubricants include stearic acid, barium stearate, magnesium stearate, talc, hydrogenated oils, waxes and the like. Useful antioxidants include tocopherols, dibutylhydroxyindole, butyl hydroxy oxybenzene, ascorbic acid, and the like. Those skilled in the art to which the present invention pertains can turn over the appropriate components of the appropriate composition according to the known techniques and the contents of the present specification. The immunomodulatory composition of the present invention can be administered directly to an individual, or as a food additive, added to a food, or as a food. The immunomodulatory composition of the present invention can be used to modulate an individual's immune response. More specifically, the immunomodulatory composition of the present invention enhances natural killer cell killing/tongue production, promotes production of IFN-γ, and inhibits production of IL_4 and IL-5. Therefore, the immunomodulatory composition of the present invention helps to enhance the individual's immunity against foreign pathogens, and can reduce the incidence of inflammatory reactions, and the individual thief has considerable positive benefits. The immunomodulatory composition of the present invention can be used for the improvement of the immune function of a normal individual or a weak individual (for example, a cancer or an AIDS patient). In contrast, the word "jian" used in the text includes but foxes, humans and non-human animals, such as cattle, sheep, ethics, horses, dogs and jealousies. The following examples are illustrative only and not intended to be limiting of the invention. Examples of pharmaceutical egg protein (ovalbumin, ova), heparin (heparin), concanavalin a (ConA), lipopoly (LPS), desertification 3_ (4,5_ dimethyl order sitting_2) _2, 5 - phenyltetrazolium (MTT), dimethyl sulfoxide (DMS 〇), Freund's adjuvant 201137124 (Freund's adjuvants), Hank's balanced salt solution (HBSS), Delbecco's modified Eagle's medium (DMEM), RPMI 1640 Trypan blue, Tween-20, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich, USA. Fetal bovine serum (FB S ) is constructed from Paa Laboratories GmbH, Australia (Pasching, Australia).

The DuoSet ELISA Development System was purchased from the US R & d Systems Corporation (Minneapolis, Minnesota, USA). Data presentation and statistics

The experimental results of the following examples are shown as mean ± standard error (Mean ± SD). Each group was compared by Student's t-test (Studenfs t-test), and p < 〇.〇5 represents a significant difference. Example 1: Preparation of Helicobacter pylori The preparation of pyloric helix = bacteria was mainly carried out according to the method disclosed by Shin et al. (supra). Briefly, 'The American Type Culture Collection No. ATCC 435〇4 = H. pylori cultured in Brucella broth (brucdla br〇th, Difco Experiment 2 'Detroit City 'Michigan' USA), which contains 5% Fetal bovine serum and antibiotics including 2.5 pg/ml of amphotericin B, 1〇μ§/ιη1 of vancomycin, 5 ΪΪ1 of trimethylammonium sneeze; and 2.5 IU/ml of succulent sputum, all purchased from ==na Chemical Company), culture conditions are 37. 〇, 1〇% c〇2 and 2^ speed. Remove the cultured Helicobacter pylori and centrifuge at 12, _ χ g for 1 〇 sonic wave ° f after centrifugation 'to obtain the supernatant' This is the pylorus p. dry with a cinchonnic acid method (bicinchoninic acid method,

Mrce, R0ckford, ΠΙ) measures the protein concentration of Helicobacter pylori lysates. (4) 2 1_Maternal Difficulty and Unaccountable Yellow Dry Powder (10) Take the pyloric helix lysate (mu US) of Example 1 and submit it to the hen by intramuscular injection. B_(10) one, - (5). Per-hen:=Up 201137124 There are 4 positions to receive the injection, each position is 25〇 μ1. After the first injection, a second enhancement reaction was carried out every two weeks with a Helicobacter pylori lysate mixed with Freund's incomplete adjuvant. One month after the last enhancement reaction was completed, the eggs laid by the hens were collected, and the yolk portions were taken out and lyophilized to obtain an lyophilized powder of yolk (hereinafter referred to as "IgY-HP"). Further Example 3: Treatment of mice L-source of mouse and its feeding management The mice used herein were 6-week-old, female BALB/c mice, which were purchased from Lesco Biotech Co., Ltd. (BioLASC). 〇Taiwan Ca, Ud,

Taipei, Taiwan). The feeding management of experimental mice was approved by the Animal Experimental Management Team of the Research Institute of Food Industry in Hsinchu City. In short, the mice were observed for two weeks, and the abnormalities (such as photophobia, dehydration, etc.) or the experiments falling outside the mean body weight ± 2 standard deviation were eliminated. Small United States ^EPCO company (Warrensburg, ΝΥ) experimental animal-specific wood filings, after the use of bacteria, replaced twice a week. The experimental feed is the PMI article of the experimental atmosphere of the American Purina Company. The breeding environment was 23 ± 2 t: temperature, 5 〇 ± 10% relative humidity and 12 hours of light and dark alternating. 2. Feeding experiment of mice The mice were randomly divided into two groups, each group was only one group, and one group was defined as yep. The mice exposed to the HP sputum were taken from about 2.8 ml of deionized water to form a sand suspension, and the suspension was orally administered to the mother for 10 weeks (from 6 weeks to 16 weeks). Week,), = for the mice in the water-receiving group, the daily feeding amount of deionized water. 3. Immunization experiment in mice = small milk also received * Gva to induce immunity during #食. In detail. ^ 7 In the pure food experiment, the blood is collected from the tail first, and then you are arrested at 13 weeks of age and '3 times of immunogenic side, which is mixed with if, if, adjuvant, and administered to mice by subcutaneous injection. No use, and ova as the antigen and incomplete Freund's adjuvant 2 201137124 3 combined with the main shot to the small 5, the second and third immunogenic effects. The second product i i〇°〇vm^i2 Mg, 1 ton and 1 〇, each time the injection body is _μΙ. Blood is collected from the tail periodically after maternal immunogenicity. After the mice were finished at the age of 16 weeks, the whole body was sprayed with alcohol and moved to biosafety operation. I:

5 steps to take the spleen. Table 1 shows the treatment of the mouse in the preparation of Tai 1: mouse virtual (four) Syrian J experiment mice

Time

Adapt to the environment, weigh the cage, collect blood, start irrigation

1 7 2 8 3 9 4 10 5 11 6 12 7 13 8 14 9 15 10 16 Food 1st immunogenicity Blood collection 2nd immunogenicity Blood collection 3rd immunogenicity

Example 4: Mice's sputum re-pointing # The pq was observed for ten weeks as described and the growth of the mice was observed. Table 2 g's weekly body weight measurement of mouse body Table 2: mouse body weight measurement results, gas weight measurement results. 201137124 Experiment Time (Week) 〇^ 1 2 3 4

Water control group (n=12)

IgY-HP experimental group (n=12) 6 7 8 9 10 11 12 13 14 15 16 20.9±1.0 21.3±0.7 19.6±0.6 21.2±1.〇22.2±0.9 23.4±0.7 24.7±0.8 25.1±0.8 25.2±0.7 25.8±1.2 h results in a flat touch 2. * indicates a significant difference with the water dragon group (p < 0.05) and the early morning position is gram (gm > 卞 Table 2 does not 'the weight appears below the water during the 6th to 8th week The phenomenon of the control group, but the subsequent 9 /=== Iger experimental group and the water control group of the mouse body $ to = week = sex, regardless of the feeding composition. v 5 6 7 8 9 10 20.9 ± 0.7 21.4 ± 〇.3 19.8±0.6 21.3±〇.722.2±〇.6 23·1±〇.7 23.7 soil 0.6* 24.1±〇.6* 24.8±0.7* 25.1±〇.6 25.3 ± 0.6 Example 5: Non-specific Analysis of the anti-tuberculosis director. The whole blood obtained after the completion of the ten-week feeding experiment was saved in 4 C ' antibody detection reagent (Mouse Ig ELISA Quantitation Kit, Bethy丨, Montgomery, Texas 'USA)' The total amount of IgG antibodies contained in the blood was determined by sandwich enzyme-linked immunosorbent assay ( sandwich-ELISA) according to the instructions. Table 3 shows the non- The measurement results of the total amount of lgG antibodies heterosexual Table 3: nonspecific antibody measurement result of the total 201 137 124

Total IgG antibody (Hg/mL) Water control, group (n=9) The first immunization before immunization The second immunization The third immunization 57 Shi 12 120±14 344±99 830 ±246

IgY-HP experimental group __(η=10) 51±8 133±26 533±173* 1010±317 1·Results are expressed as mean + standard deviation (mean+ SD) 2. * indicates significant difference with water control group difference. ', the amount of extraction can promote the total amount of antibody in the blood of mice (after the second immunization), the average of the total amount of IgG antibodies in the third immunization test group is still higher than the water control group ( There is no statistical difference between the two, which is presumed to be caused by premature activation). Example 6 · Analysis of the killing activity of non-specific natural killer cells s Λίίί into the small ship food experiment _ spleen taken after the sacrifice, placed in m towel. Xiang syringe advancement||At the end of the flat, she pressed the spleen and shattered it until the color turned white', indicating that the spleen cells between the connective tissues were free, and the liquid towel was cultured. Miscellaneous, the culture containing the sense cells is taken to a centrifuge tube and allowed to stand for 5 to 10 minutes, so that connective tissue or large particle debris is precipitated below. Thereafter, the upper cell suspension was pipetted into another centrifuge tube and centrifuged at 25 Torr xg for 5 minutes. After discarding the supernatant, pat the wall to spread the cells at the bottom of the tube. Add 5 ml of ice-cold ACK red blood cell disruption buffer, and evenly mix with the cells in the tube for 1 minute, then add 5 ml of the warmed and serum-containing medium. Then, the cells were centrifuged at 250 xg for 5 minutes, and the supernatant was discarded, and then washed twice with 1 〇 ml of HBSS buffer. The cells were suspended in 1 ml of the medium. The concentration of the cell suspension was calculated by trypan blue staining. The natural killer cell cytotoxic activity in the spleen cells of the small 201137124 mouse was examined using a cytotoxicity assay kit (LIVE/DEAD cell-mediated cytotoxicity kit ‘American Molecular Probes Inc., Eugene, USA). In short, 'first use the dye DIOC18 marker natural killer cell target cell Ya〇l (37〇C, 5% C02 incubator for 16 hours), then spleen cells and target cells (E/T) 1 : 100 or 1: 200 ratio mixing, in a 37 ° C, 5% C 〇 2 incubator, for 4 hours. In the negative control group, spleen cells were mixed with the target cells in rpmi 1640 medium (containing 1% fetal bovine serum). The number of cells was analyzed by flow cytometry. The natural killer cytotoxic activity (%) was calculated as "the target cell death rate of the experimental group and the target cell death rate of the negative control group". Table 4 shows the results of analysis of natural killer cytotoxic activity. Natural killer cell active water control group IgY-HP experimental group (%) (n=10) (n=10) natural killer cell activity f〇/n) E/T=100:l 35.2 ±3.4 39.0 ±5.0 E/T =200:1 39.9 ±3.1 45.8 ±5.3* 1. The results are expressed as mean + standard deviation (mean + SD). 2. * indicates a significant difference from the water control group. Table 4: Natural killer cell activity, 妍Cobalt Wu φ As shown in Table 4, the natural killer cell activity of the lgY_HP experimental group was significantly higher than that of the water control group (p<〇.〇5), indicating that IgY_HP has a natural killer. The role of cytotoxic activity. Example 7: Non-specific cytokine secretion assay In a 24-well culture dish, a culture solution (control group), a culture solution containing ConA (final concentration) or LPS (final concentration: 1 〇μδ/ιη1) was added, respectively. The spleen cell suspension of Example 4 was added, and the total number of cells per well was 〇4×10 cells. The cells were cultured for 24 and 48 hours in a 37 ° C, 5% 0) 2 incubator, and the cell culture supernatant was collected. Using the cytokine test kit ELISA Devel〇pmentSystem, USA R&D Systems, Minnesota, 'Minnesota' USA, USA, sandwich enzyme-linked immunosorbent assay, sandwich-ELISA, measurement The content of non-specific cytokines 'IL-2, IFN-γ, IL-4 and IL-5 in the cell culture supernatant. Table 5 shows the measurement results of non-specific cytokines. Table 5. Measurement of non-specific cytokines Water control group IgY-HP experimental group-----(n=10) - (n=l〇) IL-2 (pg/mL) Culture medium 17±99 ConA 9423±2463 LPS 19±6 IFN-γ (pg/mL) Culture medium 〇±〇ConA 3013±814 LPS 960±529 IL-4 (pg/mL) Culture medium 0±0 ConA 337±189 LPS 12±2 IL -5 ( pg/mL ) υ±υ

13±7 10225±2099 18 ±3 0.37±1.18 3549±1213 1513±658 〇±l 349±153 9±2*

Tussah ConA LPS υ±υ 260±93* 26±7 470±272 -----25±5 L The result is expressed as mean + standard deviation (mean + SD) „ 2. * indicates between the water control group and the water control group There was a significant difference., Fetal preparation 5 / kg not 'Compared with the water control group, IgY4iP experimental group of Th2 fine inhibition of sputum secretion is significantly lower (P < 〇. 〇 5); shows that 1gY has 虿 inhibition of Th2 immune response Example 8: Specific cytokine analysis 15 201137124 In the same manner as in the above Example 5, the specific 'in a 24-well culture dish' was separately added to the culture 2. The simple (final Han degree was 100 pg/ml) culture. Liquid, and add $^^) or 〇va cell suspension, so that the total number of cells per well is 〇·4 x 1〇6 έ 』 4 spleen fine 〇 2 培养 2 2 2 2 培养 , , , , , , , , , , , , , , , , , Using the aforementioned cytokine test kit, the measurement results of human dendritic T-specific cytokines of cell culture cytokines IL-2, IFN-γ, IL-4 and IL-5 were measured. 3 s ° Table 6 shows specificity

Indicates Table 6: Measurement of specific cytokines Water control group 50±12 535±19〇19±6 169±86 IL-2 (pg/mL) IFN-γ (pg/mL) IL-4 (pg/mL) IL-5 (pg/mL) _____ L results in mean + standard deviation (mean + ; 2. * indicates a significant difference between the water control group - the table IgY: HP on the one hand can inhibit ova-specific IL_4 and 1L-5 (P < 〇〇 5, 2 can promote the secretion of 0-specific IFN_丫 significantly increased = IgY_HP can promote the secretion of specific TM cytokines, reduce the secretion of specific plus cytokines. Examples from %1 Powder conversion - hormone analysis, tamping:, 丨 股份有限公司 购 购 购 购 购 购 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 I I I I I I I I I I I Combine the powder and load it into the capsule (the weight of each capsule is divided into the actual way, the mouse meal is carried out. In short, the mice are 8 to 12 with the group, the group is the water-based control) Group, another experimental group of 'gY-HP combination powder. With 7 〇 kg of adults and 16 201137124 words, as a general health use, the recommended amount is 2 capsules per day.

Capsules 'converted to mice (body weight 20g) dose is about 2 j W for the experimental group: sputum, gas, take the above dose of Ι ΥΉ ΥΉ ρ combination powder = ml of deionized water to form an immunomodulatory suspension, daily The suspension was 'for several tens of weeks (from 6 weeks to 16 weeks of age); and the mouse was given a daily dose of deionized water. The aforementioned small approach accepts ova immunogenicity during feeding. Example 3 According to the method of the above Example 8, the percentage of the cytokine of the specific cytokine in the water control group was increased or decreased, and the cytokine of the 卞^ group was compared with the healthy surface of the Jianshui test (experimental value/water control) The cytokine measurement value of the group is multiplied by the difference between the value of the hundredth of a hundred percent. . Table 7 shows that the specific cell cytokine of the igY-HP combination powder is more than the IMil suture group) IL-2 (pg/mL) IFN-γ (pg/mL) IL-4 (pg/mL) Increased by 12%, increased by 40%, decreased by 68%; decreased by 59%51 • '----- One IL-5 (pg/mL)__ * indicates a significant difference from the water control group. - A separate cytokine fraction of mulberry yellow and chitosan compared to the cytokine of the water control group increased or decreased by 100: ratio; Cytokine cytokine changes in rabbit jj mulberry and chitosan increased IFN-γ (pg/mL) by 38%* knot! L-4 (pg/mL) decreased by 1〇% decreased by 12% 201137124 —ikLJpg /mL) Xian NO, showing ^^ line test: -----1 drop 23〇 / 〇 * indicates a significant difference between the water control group and the water control group. 2 ‘and reduce the briberyTM cytokine's bifurcation. The effect of mulberry yellow or chitosan alone. Money =, the IgY_HP of the present invention proves to enhance the natural killer cell toxicity, such as the secretion of IFN-γ and inhibit the secretion of IL_4 and IL_5; and the effect of combining mulberry yellow and chitosan is better than single mulberry yellow or The effect of chitosan alone. The immunomodulatory composition of the present invention was first proposed and has been shown to promote the immune response of individuals to the Th1 immune response, avoiding the occurrence of Th2 inflammatory response, and is quite beneficial for combating foreign pathogens and reducing inflammatory response.

Claims (1)

201137124 VII. Patent application scope: i - an immunomodulatory composition comprising an egg yolk product, which is produced by a female bird of the group: Helicobacter pylori (polar sand/w>/), a fragment thereof or a group immunized egg. 4. The composition of claim 1, wherein the bird is a chicken, a duck, or a crane. 3. The composition of claim 3, wherein the egg yolk product is a freeze-dried powder. The composition of claim 1, which is in the form of a capsule, a tablet, a sharp form, a pill form, a suspension form, or a granule form. 5. The composition of claim 4, wherein the egg yolk product is a freeze-dried powder incorporated into the capsule. 6. The composition of claim 1, which is in oral form. 7. The composition of claim 1, wherein the individual is a human. 8. The composition as claimed in claim 1 which further comprises mulberry yellow and chitosan. 9. The composition of claim 1, wherein the weight ratio of the egg yolk product to the chitosan is about 2:2:1. The composition of any one of claims 1 to 9 which can be used as a counterfeit additive or food. ‘· 19 201137124 IV. Designated representative map: (1) The representative representative of the case is: ( ). (2) A brief description of the symbol of the representative figure: (none) 5. If there is a chemical formula in this case, please reveal the chemical formula that best shows the characteristics of the invention: (none) 2