TW201119631A - An injection method for shrimps - Google Patents

An injection method for shrimps Download PDF

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TW201119631A
TW201119631A TW098141837A TW98141837A TW201119631A TW 201119631 A TW201119631 A TW 201119631A TW 098141837 A TW098141837 A TW 098141837A TW 98141837 A TW98141837 A TW 98141837A TW 201119631 A TW201119631 A TW 201119631A
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Taiwan
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shrimp
injection
organism
cavity
injecting
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TW098141837A
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Chinese (zh)
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TWI400064B (en
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Ying-Nan Chen
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Univ Nat Pingtung Sci & Tech
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  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)

Abstract

An injection method for shrimps comprises a time-released step, an ice-anesthetized step and a cavity injected step. In the time-released step, a injecting medication is mixed with a covering oil in order to obtain a time-released medication. In the ice-anesthetized step, a shrimp is kept at a cold environment for temportary anesthetization. In the cavity injected step, the time-related medication is injected into a cavity which is located in a basal part of a rostrum placeing on a carapace of the shrimp. Therefore, the time-released medication can slowly release into the body of the shrimp.

Description

201119631 六、發明說明: 【發明所屬之技術領域】 本發明係關於-種蝦類生物的注射方法,特別是關於 一種可以減少注射致死率的蝦類生物注射方法。 【先前技術】 蝦類養瘦業曾是台灣重要經濟來源的一部分,其重要 性不下於農業之其他產業;自六十年代起,由於養殖技術 之突破,蝦類養殖的產業發展一日千里,由早期的魚瑕混 養演進至蝦類單養,興盛時期因人工飼料開發成功而邁由 集、力式養殖’為台灣打開養瑕產業之國際市場;然而後期 卻因產業膨脹過劇引發養殖環境嚴重惡化,爆發病毒威染 的問題,造成養瘦蝦類大量死亡,以致產業萎縮,昔日的 風光已不復見’尤其是草瑕養瘦,近年來,雖有多數專家 學者致力於病毒防治的研究及健康種瑕的培育,試圖挽救 頹勢,然而成效十分有限。 現今瑕類養殖主要的研發重點,為針對目前產業所遭 j的種種問題,尋求改善途徑’主要多著重於如白點症病 毒(white spot syndr⑽e vims WSSV)、桃拉病毒(丁議 Syndrome virus,TSV))等感染瑕類病毒的致病性或疫苗試 驗’蝦類免疫激活劑的開發以及瑕類生殖性腺 關研究,多數實驗為考量所試驗藥劑本身的特性,㈣才為 準確的控制藥劑的植入劑量及觀察養殖蝦類對於該藥劑試 驗的反應’需要採用注射的方式植入試驗藥劑。’、 明參照第1圖所不’係—瑕類生物外部型態之示意 201119631 圖’瑕類生物屬於節肢動物,其外表可分為頭胸部1及腹 節2兩大部份’蝦類生物係為開放式循環系統,血液與組 織液扣合成血淋巴(hemolymph)於體腔中由心臟壓縮提 供動力循流。蝦類生物的心臟一般係呈黃褐色或淺褐色, 主要位於肝胰臟(hepatopancreas)後端上方,緊貼於頭胸 邻後螭背曱下方;而習知蝦類生物的注射方式,為避免針 頭刺入時,傷及重要組織或器官如圍心腔、心臟等,影響 蝦類生物的正常生理,故多由蝦類生物的第一腹節2丨處的 肌肉植入施打藥劑,由肌肉吸收藥劑,觀察蝦類生物的反 應。 然而,以腹卽肌肉注射的方式,雖然可以避開心臟’ 但是注射部位的準確度有時難以掌控,同時,注射肌肉對 蝦類生物來說係一種直接的刺激,多數蝦類生物因無法承 受注射時的緊迫性而在施打後死亡,另外,腹節肌肉部位 /又有一足夠的施樂空間’單次可注射藥物之劑量、體積較 小(一般少於0.1H1L ),若需試驗一些較高劑量或較大體積之 藥物,或是一些體内代謝率快而需大量注射或多次注^的 成分,則容易因頻繁的注射率及高量藥劑的負擔而導致更 嚴重的致死率’有試驗上的困難;由上述缺點,習知瑕類 生物的注射方法無法施予較高劑量或較大體積的率劑,注 射致死率高且有多次注射的困難,故在試驗藥劑上往往受 限過多,造成養殖瑕類的實驗瓶頸,有再精進二必要性^ 以推動蝦類養殖進一步的研究發展。 【發明内容】 201119631 本發明主要係改良習知蝦類生物注射技術之缺點,提 供-穆蝦類生物的注射方法,简改善蝦類生物因藥物注 射所造成之高致死率。 ' 、本發明次一目的係提供一種蝦類生物的注射方法,可 以提高針對瑕類生物單次注射之劑量及體積。 本發明再-目的係提供-種_生物的注射方法,可 以延長藥劑於個體内的反應時間。 、本發明又一目的係提供一種银類生物的注射方法,可 以避免多次注射的麻煩及所造成的緊迫效廡。 為達到前述發明目的,本發明所運用讀術手段及夢 由該技術手段所能達到之功效包含有: 曰 使 一種_生物軌射方法,係包含:―缓釋步驟,係 字-施打_混合—包裹油f,而得—具有缓釋效果 =劑;-觸冰步驟,將-蝦類生物置於—低溫環境,暫 ===類生物;及—空腔植人步驟,將該__^ 式植入該蝦類生物位於頭胸部劍額基部之空腔, 5亥緩釋藥劑逐漸釋放於該蝦類生物之體内。 【實施方式】 為讓本發明之上述及其他目的、特徵及優點能更明顯 S下文特舉本發明之較佳實施例’並配合所附圖式, 作詳細說明如下: 、印芬照第2圖所示,係本發明一種蝦類生物的注射方 去之麵作步驟流程,包含以下步驟:一緩釋步驟S1 ; —觸 冰步禪s2,及—空腔植人步驟s3。 201119631 本發明一種蝦類生物的注射方法之緩釋步驟Si,係將 一施打藥劑以一定體積之比例混合一包裹油質,使該包裹 油質可以包覆於施打藥劑之外層,而形成數個施打藥劑之 包覆顆粒,該包覆顆粒可以減緩顆粒内該施打藥劑所釋出 的速率,係為一緩釋藥劑,具有緩釋效果;該施打藥劑之 廷用可以依照試驗項目之不同而選用各種藥劑,而包覆於 外層的包裹油質可以選自由一甘油'撖禮油、植物油及不 完全佐劑所組成之群組。201119631 VI. Description of the Invention: [Technical Field to Which the Invention Is Applicable] The present invention relates to a method for injecting shrimp-like organisms, and more particularly to a method for injecting shrimp by biological injection which can reduce the lethality of injection. [Prior Art] The shrimp thinning industry was once part of Taiwan's important economic source, and its importance is no less than other industries in agriculture. Since the 1960s, due to the breakthrough in farming technology, the shrimp farming industry has developed rapidly. The breeding of the surimi has evolved to the single-cultivation of shrimps. During the booming period, due to the successful development of artificial feed, the development of the cultivation and the cultivation of the farms has opened the international market for raising the industry. However, in the later period, the industrial environment has been severely affected due to the expansion of the industry. Deterioration, the outbreak of virus-dyeing, causing a large number of deaths of thin-skinned shrimps, resulting in a shrinking industry, the past scenery is no longer seen, especially in the grass-roots, in recent years, although most experts and scholars are committed to virus prevention research And the cultivation of healthy species, trying to save the situation, but the results are very limited. The main research and development focus of today's aquaculture is to find ways to improve the problems faced by the current industry. 'The main focus is on white spot syndr (10) e vims WSSV) and the peach virus (Syndrome virus). TSV)) Pathogenicity or vaccine test for infection of prion viruses 'Shrimp immune activator development and steroidal glandularity study, most of the experiments consider the characteristics of the tested drug itself, (4) is the accurate control of the drug Implantation dose and observation of the response of cultured shrimp to the drug test 'requires injection of the test agent by injection. ', Ming with reference to Figure 1 is not 'systems' - the external type of scorpion organisms 201119631 Figure 'Apes are arthropods, the appearance can be divided into two parts of the head chest 1 and the abdominal section 2 'shrimp organisms The system is an open circulatory system, and blood and tissue fluids are combined to synthesize hemolymph in the body cavity to provide power circulation by cardiac compression. The heart of shrimp organisms is generally yellow-brown or light brown, mainly located at the back of the hepatopancreas, close to the back of the head and chest, and the way of injection of shrimps is avoided. When the needle is pierced, it damages important tissues or organs such as the pericardial cavity and heart, affecting the normal physiology of the shrimp organism. Therefore, the muscles of the first abdominal segment of the shrimp organism are implanted with the drug. The muscle absorbs the agent and observes the reaction of the shrimp organism. However, the intramuscular injection of the diarrhea can avoid the heart', but the accuracy of the injection site is sometimes difficult to control. At the same time, the injection of muscle is a direct stimulation of the shrimp organism, and most shrimp organisms cannot withstand the injection. The urgency of the time and death after the application, in addition, the abdominal muscles / there is enough Xerox space 'single injectable drug dose, small volume (generally less than 0.1H1L), if you need to test some higher Dosage or larger volume of drugs, or some components with high metabolic rate in the body that require large injections or multiple injections, are more likely to cause more serious mortality due to frequent injection rates and high doses of drugs. Difficulties in the test; due to the above shortcomings, the injection method of the known mites can not be administered with higher doses or larger volume agents, and the injection mortality rate is high and there are multiple injections, so the test agents are often subject to Too many limits, resulting in experimental bottlenecks in the cultivation of mites, there is a need to refine the second need ^ to promote further research and development of shrimp farming. SUMMARY OF THE INVENTION 201119631 The present invention mainly improves the shortcomings of the conventional shrimp bioinjection technology, and provides an injection method for the shrimp-like organism, which simply improves the high mortality rate caused by the injection of the shrimp organism. The second object of the present invention is to provide a method for injecting shrimp organisms which can increase the dosage and volume of a single injection for a moss. A further object of the present invention is to provide an injectable method for prolonging the reaction time of an agent in an individual. Another object of the present invention is to provide a method for injecting silver-like organisms, which can avoid the trouble of multiple injections and the resulting urgency. In order to achieve the foregoing object, the functions of the reading method and the dream that can be achieved by the technical means of the present invention include: 曰 a method of _ biological orbiting, comprising: "sustained release step, "--" Mixing-wrapping oil f, but having a slow release effect = agent; - ice-touching step, placing - shrimp organisms in a low temperature environment, temporarily ===like organisms; and - cavity implanting steps, _^ The implanted shrimp organism is located in the cavity of the base of the head and chest, and the 5 liter sustained release agent is gradually released into the body of the shrimp. The above and other objects, features, and advantages of the present invention will become more apparent. The figure shows a step-by-step procedure for the injection of a shrimp organism according to the present invention, comprising the following steps: a slow release step S1; a touch ice step s2, and a cavity implant step s3. 201119631 The slow release step Si of the method for injecting shrimp organisms according to the present invention is characterized in that a coating agent is mixed with a package of oil in a certain volume ratio, so that the oil can be coated on the outer layer of the application agent to form an outer layer of the application agent. a plurality of coated granules for applying the granules, wherein the coated granules can slow the release rate of the medicinal agent in the granules, and are a sustained release medicinal agent having a sustained release effect; the medicinal agent can be used according to the test item. The various agents are selected differently, and the encapsulated oil coated on the outer layer may be selected from the group consisting of glycerol's eucalyptus oil, vegetable oil, and incomplete adjuvant.

灵評言之 腺發育之一生物胺,對一草蝦進行注射試驗;係將該生物 胺作為該施打藥劑,以等體積之比例均句混合一甘油作為 該包裹油質,形成該緩釋藥劑;擬將該生物胺植入一草瑕 個體進行試驗時,該包覆顆粒可以做為該生物胺 蝦個體之—輸送紐,使該生物胺可以穩定植人-特^部 位,並且於該特定部位緩缓釋放至草蝦體内,可延長該生 物胺於體内作用的時間,發揮緩釋效果。 / 本發明一種瑕類生物的注射方法之觸冰步驟^,係將 ==生物置於一低溫環境上,而使該瑕類生物之 於二/ 转反應,本實施例係將—草蝦個體靜置 二堍ί數秒,使該草瑕之—側表面可直接接觸於該 〜冰塊時感覺麻如減緩其 藥劑之操作順暢。 勃力^便稍後植入 本發明一種蝦類生物的、、 S3,請參照第3及第4圖奸入步驟 方式植入該r刪頭胸::之:;,劑’由注射 之d領11基部,蝦類生物頭 201119631 胸j 1之劍《1 u基部具有一空腔12 (如第4圖n 揮該緩釋藥劑腔12内緩慢釋放至瑕類生物體内,發 由上所述’本發明一種瑕類 視_型甚可: 可以增加單姐射所能植人的藥量 。、里 具有緩釋效果,可以 $卜触射之緩釋藥劑 内所作用的時間,減少=:=夏=長個體 期嫌咖_,減少注射=率避免瑕類生物因長 注射緊為'Γ/^發明一種瑕類生物的注射方法可以有效改善因 90.7±6八彡養1目月之難物,該草蝦之平均體重為 t ’有病毒感染及其他明顯的生理問題,將該數隻 瑕_為三組’—觸雜緩釋雖),係舰本發明—種 、物的注射方法,植入一緩釋藥劑’本實施例之緩釋藥 ^、糸以一生物胺為施打藥劑而製成;該緩釋藥劑之注射劑量 =。別_ 一低劑量及一高劑量’該低劑量之藥量係為該高劑 你一/°L分別注射於草制_體顯部觸基部之空腔内;另操 ^腹節肌肉緩釋組⑻,係採用相同之緩釋藥劑(生物胺),同 :將草瑕個體經本發明之觸冰步驟%麻醉後再注射,注射部 則係於第-腹節之肌肉部位,所植入緩釋藥劑之注射劑量則 201119631 與該劍額空腔緩釋組(A)之注射情形相同;_劍額空腔生理組 (Q,則係將該生物胺溶於—生理食鹽水,配製—連續低劑量 =懸係為紐劑量之_及—連續高賴其劑量係為 =劑量之職)之生物胺溶液,該賴低婦與該連續高劑 量比為誦,每隔四天注射於草瑕劍額基部之空腔, =額空时雜(C)於輕期__辑讀綱量,不 額⑷及該㈣肌肉所注射之 後^或⑤齡,觀祭並紀錄上述三組草蝦分別於注射試驗 各組存活的情形,結果紀錄於下表: 施藥量 低劑量 存活率_空腔、緩腹節肌肉、缓_空腔生 釋組(Al)!〇)釋組巧(%)理组 40 高劑量 —---- 連續低劑量 .....................~~~-____ 連續高劑量 30One of the glandamines of the gland development of the spirit evaluation, an injection test is carried out on a grass prawn; the biogenic amine is used as the application agent, and a glycerin is uniformly mixed in an equal volume ratio to form the sustained oil release agent; When the biogenic amine is to be implanted into a grasshopper individual, the coated granule can be used as a transporting bacterium of the biogenic prawn individual, so that the biogenic amine can stabilize the phyto-existing part and at the specific part. Slow release into the grass shrimp can prolong the time of action of the biogenic amine in the body and exert a sustained release effect. / The ice-touching step of the injection method of the steroid organism of the present invention is to place the == organism on a low temperature environment, and the mites are subjected to a second/revolution reaction, and the present embodiment is a grass prawn individual After standing for two seconds, the side surface of the grass can be directly contacted with the ice cube to feel the ease of operation of the medicament. Bo Li ^ will be implanted later in a shrimp organism of the present invention, S3, please refer to the 3rd and 4th figure into the step of implanting the r-cut chest::::, the agent 'by injection d Collar 11 base, shrimp biological head 201119631 chest j 1 sword "1 u base has a cavity 12 (as shown in Figure 4 n) slow release of the drug cavity 12 slowly released into the steroid organism, issued by 'The invention is a kind of scorpion _ type s: can increase the amount of medicine that can be implanted by a single sister. It has a slow release effect, and the time spent on the sustained release drug can be reduced by = = summer = long individual period y _, reduce injection = rate to avoid sputum organisms due to long injections tightly 'Γ / ^ invented a scorpion organism injection method can effectively improve the difficulty of 90.7 ± 6 彡 1 1 The average body weight of the grass shrimp is t 'virus infection and other obvious physiological problems, the number of 瑕 _ is three groups '-------------------------------------------------- Implanting a sustained-release drug's sustained-release drug of the present embodiment, and sputum is prepared by using a biogenic amine as a pharmaceutical agent; the dose of the sustained-release drug is injected =. _ _ a low dose and a high dose 'the low dose of the dose is the high dose of you / °L respectively injected into the cavity of the grass _ body part of the base; Group (8) adopts the same sustained-release drug (biamine), and the same: the grasshopper individual is anesthetized by the ice-touching step of the present invention, and the injection part is attached to the muscle part of the first-abdominal section, and the implant is slowed down. The injection dose of the release agent is the same as that of the injection of the Sword cavity slow release group (A) in 201119631; _ Sword cavity cavity physiological group (Q, the biogenic amine is dissolved in the physiological saline solution, formulated - continuous Low-dose = suspension of the dose of ton and - continuous high reliance on the dose of the dose of the dose of the biogenic amine solution, the Lai low woman and the continuous high dose ratio of sputum, every four days into the grasshopper The cavity of the base of the sword, = the amount of time and space (C) in the light period __ reading the amount, not (4) and the (4) muscles after injection ^ or 5 years old, observe the sacrifice and record the above three groups of grass shrimp The survival of each group in the injection test, the results are recorded in the following table: Application rate Low dose survival rate _ cavity, slow abdominal muscles, slow _ cavity Release group (Al)!〇) Release group (%) group 40 high dose ----- continuous low dose........................~~ ~-____ Continuous high dose 30

草^上表結果所示,同樣植人__(生物胺)之二組 旦的古’ ^劍财腔部健狀草瑕⑷,*論所植入劑 广低’冑有7Q%以上的縣^纽射 :良好的存活狀態,而接受第一腹節之肌肉注射的草: 之^直人低劑量之草蝦僅有活存,而提高注射劑量 空月I其^使所注射草社存活料低至鳩,·而於劍額 工上土部植入生物胺溶液的草蝦(c),不論所連續注射之劑『 201119631 3里〇:)低由?二二射試驗結束後大量死亡(存活率僅有 明二,節肌肉注射的方式植入本發 苴以挪术白勺確會對草瑕個體造成刺激,以致多數 草蝦個體於注射後死亡.,品夕数 可以減_#個h 判之觸㈣注射方式則 ^對相個體的注射刺激,減少死 採用未經緩釋處理之筚兩丨 右 J主射於卓蝦劍額基部的空腔,則 期再措-:背可於草瑕個體内之作用時間有限,須定 體“迫:=生然而多次且頻繁的注射同樣造成草瑕個 期間死衡,同樣致使多數草蝦個體相繼於試驗 承上述結果,本發明之植人蝦類生物劍額基部的注射 /’不會對所接受注射的糊個體造成明顯的致死率, 德^使該_個體接受較大的注射劑量也可以於注射 後保持★良好的生理狀況;另外,本發明緩釋藥劑,係 劑可於蝦類個體内產生緩釋效果,延長該藥劑之 T間’避免重複施打對蝦類生物所造成之負面影響, 故應用本發明可以有效改善瑕類生物的注射致死率,同時 避免多次注射對蝦類生物的刺激,有利於瑕類生物藥劑注 射試驗的進行。 Θ / 另外,本實施例所採用的施打藥劑係—生物胺,係可 =誘fx瑕類生物之性腺發育,該生物胺無法直接由食物中 攝取,必須以注射方式植入蝦類生物之個體,而該生物胺 於生物體内代謝迅速,若直接以生物胺溶液之形式植入, 無法長時間作用’需多次施打以維持體内的作用量,而多 次注射易導致大量注射個體的死亡’故無法完』生物‘ 201119631 藥織驗之實驗技術;利用本發明-種蝦類生物的注射方 法’將該生物胺以緩釋藥劑形式植入瑕類個體,有利於該 生物胺於瑕類個體之長效作用,發揮生物胺誘發瑕類性腺 發展之功能;另外,該生物胺係植入瑕類頭胸部劍額基部 . 之空腔部位,該空腔部位可容納較大注射體積,省去操作 • I多次注射的麻煩,同時避免瑕類個體®多次注射而造成 死亡,故可成功進行生物胺相關的藥劑試驗。 由上所述,利用本發明一種蝦類生物的注射方法操作 •—生物胺對一瑕類生物性腺發展之誘發試驗,可成功將生 物胺植入,不造成锻類個體之死亡,並且,於試驗期間可 明顯觀察該生物胺對誘發個體性腺發展之結果,顯示該生 物胺係成功植入體内,於體内緩慢釋放且作用於特定部 位/因此,利用本發明不僅可以減少蝦類生物因注射過程 所導致的向致死率,也同時擴大適用於注射試驗之藥物範 圍,^善一些代謝速率快、作用劑量高或是需要試驗週 鲁 I之藥物測試品質,對於未來錢賴病毒的相關研究、 瑕類免疫,活劑的開發試驗及蝦類生殖能力的相關研究皆 有正面的貝獻’有助於推動瑕類養殖產業進一步的研究發展。 本發明一種蝦類生物的注射方法,係將一緩釋藥劑I ㈤至生物頭胸部之劍額基部,避免肌肉注射對瑕類生 * &所造成—,②善蝦類生物因藥劑注射或多次注 造成之高致死率,為本發明之功效。 本!X明種瑕類生物的注射方法,係將一緩釋藥 射至瑕類生物頭胸部劍額基部之空腔部位,該劍額基^ 有-空腔可容___,可以提高單次注射所能植又 201119631 的劑里及體積,為本發明之功效。 本發明-種瑕類生物的注射方法,係將 射至瑕類生物頭胸部之劍額基部,該注 果,可以延長藥劑於個體内所作用的時間,減;::釋: 中所需要崎的讀,具有转㈣之魏。°购呆作 本發明-種瑕類生物的注射方法,係將 射至蝦類生物賴部之_基部 =樂制注 及注射量小的瓶頸,擴大藥物注射之試驗:圍主=: 殖瑕類研紐展之魏。 ㈣於養 /本發明已·上述較佳實施例揭示,然其並非用 以,定本發明’任何熟f此技藝者在不脫離本發明之精神 和犯圍之内’㈣上述實施例進行各種更動與修改仍屬本 發月所保4之技術範*,因此本發明之保護範圍當視後附 之申請專利範圍所界定者為準。The results of the grass ^ table show that the same group of __ (biological amines) of the two groups of ancient '^ Jiancai Department of health-like grasshoppers (4), * on the implants wide and low '胄 have more than 7Q% County ^ New Shot: Good survival, and the grass that received the first abdominal joint of the muscle: the straight human low-dose grass shrimp only live, and increase the injection dose of empty moon I to make the injected grass society survive The material is as low as 鸠, and the grass prawn (c) implanted with the biogenic amine solution in the soil of the Sword, regardless of the continuous injection of the agent "201119631 3 〇:) low? A large number of deaths after the end of the two-shot test (the survival rate is only the second, and the method of intramuscular injection of this hairpin will cause irritation to the grasshopper individuals, so that most grass shrimp individuals die after the injection. The number of eves can be reduced by _#h. The touch is (4) the injection method is ^ the stimulation of the individual injection, reducing the death using the unreleased 筚 two 丨 right J main shot at the base of the Zhuo Shoujian base, In the case of a period of time - the back can be used in the grasshoppers for a limited time, must be fixed "forced: = birth, however, repeated and frequent injections also cause the grass to die during the period, also caused most grass shrimp individuals to succeed According to the above results, the injection/' at the base of the implanted shrimp bio-sword of the present invention does not cause a significant lethal rate to the paste-injected individual, and the individual can receive a larger injection dose. Maintaining a good physiological condition after injection; in addition, the sustained-release preparation of the present invention can produce a sustained release effect in the shrimp individual, and prolong the T-between of the medicament to avoid the negative effects caused by repeated application of the shrimp-like organism. Therefore, the invention can be applied In order to effectively improve the injection lethality rate of scorpion organisms, and avoid the stimulation of shrimp organisms by multiple injections, it is beneficial to the injection test of steroidal biological agents. Θ / In addition, the application of the herbicide-biamine in this example , can be induced to induce the gonadal development of fx steroids, the biogenic amine can not be directly taken from the food, must be injected into the individual of the shrimp organism, and the biogenic amine is rapidly metabolized in the organism, if directly by the organism Implantation of the amine solution can not be used for a long time. 'It needs to be applied several times to maintain the amount of action in the body, and multiple injections can easily lead to the death of a large number of injected individuals', so it is impossible to complete the organism' 201119631 Experimental technique of drug weaving By using the invention-injection method of shrimp-like organisms, the bio-amine is implanted into the steroid type as a sustained-release preparation, which is beneficial to the long-acting effect of the biogenic amine on scorpion individuals, and exerts biogenic gonads induced by biogenic amines. In addition, the biogenic amine is implanted into the cavity of the base of the head and chest of the scorpion, which can accommodate a larger injection volume, eliminating the need for operation. The trouble of injection, while avoiding the death caused by multiple injections of the scorpion individual®, can successfully carry out the biomine related drug test. According to the injection method of the shrimp organism of the present invention, the operation of the biogenic amine is Induction test for the development of steroidal gonads can successfully implant biogenic amines without causing death of forged individuals, and the results of the development of the gonads induced by the biogenic amines can be clearly observed during the test, indicating that the biogenic amines are successful. Implanted into the body, slowly released in the body and acting on specific sites. Therefore, the invention can not only reduce the mortality rate caused by the injection process of the shrimp organism, but also expand the range of drugs suitable for the injection test. Some metabolic rates are fast, the dose is high, or the quality of the drug test needs to be tested. For the future research on the drug, the immunization, the development test of the active agent and the research on the reproductive capacity of the shrimp are positive. Beixing' will help promote further research and development of the aquaculture industry. The invention relates to a method for injecting shrimp organisms, which comprises a sustained release medicament I (f) to the base of the sword head of the biological head and chest, avoiding the intramuscular injection of the sputum-like sputum* & The high mortality rate caused by multiple injections is the efficacy of the present invention. this! The injection method of the X-species mites is to shoot a sustained-release drug to the cavity of the base of the scorpion creature's head and chest. The sword base has a cavity that can accommodate ___, which can improve a single injection. The agent and volume of the can be planted in 201119631 are the effects of the present invention. The invention relates to a method for injecting a mites, which is to be applied to the base of the sword of the head of the scorpion organism, and the result of the injection can extend the time of the action of the medicament in the individual, and the reduction::: Release: The reading, with the turn (four) of Wei. ° purchase of the present invention - the injection method of the mites, will be shot to the shrimp bio-resources _ base = music injection and small injection bottleneck, expand the drug injection test: the main == Wei of the research institute. (4) The present invention has been disclosed in the above-described preferred embodiments, and it is not intended to be used in any way that the present invention can be modified without departing from the spirit and scope of the present invention. And the modifications are still in the technical scope of the warranty of the present invention. Therefore, the scope of protection of the present invention is subject to the definition of the scope of the patent application.

12 — 201119631 【圖式簡單說明】 第1圖:蝦類生物之示意圖 第2圖:本發明一種蝦類生物的注射方法之操作步驟流 程圖 第3圖:本發明一種蝦類生物的注射方法之操作圖 第4圖:本發明一種蝦類生物的注射方法之蝦類生物頭 胸部示意圖 【主要元件符號說明】 1 頭胸部 11 劍額 12 空腔 2 腹節 21 第一腹節 13 —12 — 201119631 [Simplified illustration] Fig. 1: Schematic diagram of shrimp organisms Fig. 2: Flow chart of the operation steps of a method for injecting shrimp organisms according to the present invention. Fig. 3: A method for injecting shrimp organisms according to the present invention Operation Diagram Fig. 4: Schematic diagram of the shrimp head and chest of the shrimp method of the present invention [Main component symbol description] 1 head chest 11 sword amount 12 cavity 2 abdominal section 21 first abdominal section 13 —

Claims (1)

201119631 七、申請專利範圍: 1、 一種蝦類生物的注射方法,係包含: _ 一緩釋步驟,係將一施打藥劑混合一包袤油質,而得一 具有缓釋效果之緩釋藥劑; 一觸冰步驟,將一蝦類生物置於一低溫環境,暫時麻醉 該蝦類生物;及 一空腔植入步驟,將該緩釋藥劑以注射方式植入該蝦類 生物於頭胸部劍額基部之空腔,使該緩釋藥劑逐漸釋放 於該蝦類生物之體内。 籲 2、 依申請專利範圍第1項所述之一種蝦類生物的注射方 法,其中,該包裹油質係選自由甘油、撖禮油、植物油 及不完全佐劑所組成之群組。 3、 依申請專利範圍第1項所述之一種蝦類生物的注射方 法,其中,該缓釋藥劑之注射藥量係0.1〜〇.5mL。 —14 —201119631 VII. Scope of application for patents: 1. A method for injecting shrimp organisms, comprising: _ a slow-release step of mixing a pack of medicinal oil with a medicinal agent, and obtaining a sustained-release medicament having a sustained release effect a step of ice, placing a shrimp organism in a low temperature environment to temporarily anesthetize the shrimp organism; and a cavity implantation step, implanting the sustained release agent into the shrimp body by injection The cavity of the base allows the sustained release agent to be gradually released into the body of the shrimp organism. 2. A method of injecting a shrimp organism according to claim 1, wherein the oil is selected from the group consisting of glycerin, eucalyptus oil, vegetable oil and incomplete adjuvant. 3. The method for injecting a shrimp organism according to the first aspect of the patent application, wherein the sustained release drug is in an amount of 0.1 to 0.5 mL. —14 —
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102499785A (en) * 2011-11-02 2012-06-20 浙江大学舟山海洋研究中心 Micro-injection method for shrimp animals
CN109771077A (en) * 2019-02-14 2019-05-21 河南师范大学 A kind of micro intervention injection device of Shrimp waste optic stalk

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4857506A (en) * 1987-01-12 1989-08-15 American Cyanamid Company Sustained release growth hormone compositions for parenteral administration and their use

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102499785A (en) * 2011-11-02 2012-06-20 浙江大学舟山海洋研究中心 Micro-injection method for shrimp animals
CN109771077A (en) * 2019-02-14 2019-05-21 河南师范大学 A kind of micro intervention injection device of Shrimp waste optic stalk
CN109771077B (en) * 2019-02-14 2024-04-19 河南师范大学 Micro-intervention injection device for shrimp and crab eye handles

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