TW201111001A - Emulsification composition containing bio-available egg-albumin and its preparation manner - Google Patents

Emulsification composition containing bio-available egg-albumin and its preparation manner Download PDF

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TW201111001A
TW201111001A TW98132605A TW98132605A TW201111001A TW 201111001 A TW201111001 A TW 201111001A TW 98132605 A TW98132605 A TW 98132605A TW 98132605 A TW98132605 A TW 98132605A TW 201111001 A TW201111001 A TW 201111001A
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Taiwan
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composition
group
protein
emulsifier
egg
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TW98132605A
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Chinese (zh)
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yi-feng Gao
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yi-feng Gao
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Abstract

An emulsification composition containing bio-available egg-albumin and its preparation manner, wherein the composition contains therein: a lipid ingredient material, an emulsifier, and at least a liposome; the lipid ingredient material makes up 35-90 weight% of the composition; the emulsifier makes up 10-65 weight% of the composition; the liposome includes therein at least a bio-available egg-albumin, wherein the bio-available egg-albumin is a water soluble egg-albumin. By means of the physical processing manner, the bio-available egg-albumin can be wrapped into the liposome to form the material in the emulsified state, while the material of the emulsifier is selected from the edible material. Therefore, the present invention is directly edible and the bio-available egg-albumin cannot be made to be fast decomposed in the digestive tract.

Description

201111001 六、發明說明: 【發明所屬之技術領域】 本發明是有關於一種可直接食用之乳化組成物及其加 工方法,特別是指一種含有微脂粒的可食用乳化組成物及 其加工方法。 【先前技術】 習用之卵黃抗體的製作過程,需先將抗原打入家禽類 體内經一至二個月的補強免疫注射後,收取雞印以製備具 專一性之免疫球蛋白IgY,再純化及濃縮製成免疫球蛋白 製劑,而藉由習用製作過程所產出之IgY抗體不僅製程複 雜,且在加工過程中常因此而導致IgY抗體失去活性。 藉由上述過程所製作出的的疫苗或抗體因為其中所含 有的蛋白質具有容易因溫度、有機溶劑及不利的值而 變性等原因’因此通常以注射的方式投藥進入人或動物體 • β,以避免疫苗或疫苗因為口服進入消化道後所產生的變 性反應’而經由口服之疫苗或抗體為保護其不受生物體消 化液所降解,多以化學物質及有機溶液加工製成微膠囊, 仁如此I亦常常使抗體或疫苗中之活性蛋白,因有機溶 液之影響而失去活性。 綜上所陳傳統之疫苗或抗體的投藥方式以及其產能 上述之缺失而有待改進。 【發明内容】 201111001 成物,且在於提供—齡有微練之乳化組 便製作,覆有生物可利用性蛋白,其不僅方 添加二=態物質可直接提供為副食品,亦或食品 本發明所提供之一種含有生物可利用性 成物及其製作方法,該組成物中包含有··一脂質質且 、一乳化劑以及至少一微脂粒。 、 該脂質成分物質佔有該組成物之35至90的曹晉石八 比;該乳化劑佔有該組成物之1〇至65的重量百分比.〇 微脂粒中包含有至少一生物可利用性蛋白物 可利用性蛋白係為一水溶性蛋白。 物 藉由本發明之製作過程,可將生物可利用性蛋白包覆 於-微脂粒中,且該乳化劑係選自可食用之材料,因此本 發明係可直接食用而不會使生物可利用性蛋白於胃酸中分 解’藉此可使生物可利用性蛋白藉由口服進入消化道中而 被人體所吸收。 【實施方式】 為了詳、’明本發明之組成物及製作流程,兹舉以下 較佳實施例並配合圖示說明如後: 本發明-較佳實施例所提供之一種含有生物可利用性 蛋白之礼化組成物包含有:—乳化劑,—脂質成分物質以 及至少-微脂粒;該乳化劑佔有該經成物之1〇至65的重 量百分比;該脂質成分物質佔有該組成物之%至9〇的重 201111001 量百分比;該等微脂粒中包含有至少-生物可利用性蛋白 ,且議ml之該組成物中含有至少—分蛋 該生物可利用性蛋白。 或pg至5g的 。二==絲不限於油包水型或是水包油型 生成動物二== == 免疫特異性抗原所生成之特異性抗體 ^ ^ 群组之一,該乳化劑係選自由家禽類之卵黃 貝^加工^黃粉、攸蟲類之印黃質及其加工之即黃粉 ”、、;!之印汽質及其加工之印黃粉、植物種子萃取物、高 量咖旨f、_脂質之介面活性物質及其所域的群組之 一;該脂質成分物質係選自由食用油、嬰兒油、動物性油 、植物性油、礦物油及其所組成的群組之一。 本發明之乳化組成物係可用以製成食品、副食品或食 物調理魏劑,其能觸由進食或是制為人類或是動物 提供養分。如:沙拉,蛋黃醬等可食用之食品。 本發明之乳化組成物亦可用以製成身體表面之保養品 ’其能夠藉由塗抹於身體表面,使皮膚或嘴唇等身體表面 吸收微脂粒及其中之生物可利用性蛋白。 再請參閱第一圖,係為本發明之方塊流程圖,主要揭 示本發明之製造方法。 本發明組成物之較佳製造方法,其係包括下列步驟: (a)使一乳化劑中含有一預定量之生物可利用性蛋白 201111001 ’其中料化嶋選自由家禽類之彡卩黃質及其加工之卵黃 私爬蛾類之即黃質及其加工之印黃粉、魚類之印黃質及 其加工之彡卩讀、植物種子萃取物、高量彻旨質、類磷脂 質之"面活性物質及其所組成的群組之一;該生物可利用 性蛋白係選自㈣物減球蛋白、人卫合成胜肽、重組蛋 白、生物體内生蛋自、直接免疫㈣質生成動物體所產生 之生物可利祕蛋自、生物體經由免疫特異性抗原所生成 之特異性抗體及其所組成的群組之一。 (b)將脂質成分物質於〇它至56ΐ之間,分批加入上 述步驟之混合物申,並同時利用高壓唧筒射出、均質擾拌 等混合技術,均勻混合該脂質成分物質與該混合物;其中 該脂質成份物㈣選自由食用油、嬰兒油、動物性油、植 物性油、礦物油及其所組成的群組之一。 藉由上述較佳製造方法,操作人員於實際運作時,可 先於豕禽(泛指雞鴨鵝)體内注射入一特異性抗原,在經過 ^3個月的免疫補強注射後,上述之家禽體内於血液循環 系統中會產生抗特異性抗原之專一性抗體(Ig),而家禽體 内之卵母細胞於累積一定量的專一性抗體後形成卵黃型態 ,將此一含有專一性抗體之卵黃取出,此卵黃即為天然之 乳化劑且其中含有生物可利用性蛋白(專一性抗體),將此 卵黃分多次加入脂質成分物質,並於每次添加脂質成分物 質後以高壓唧筒射出、均質攪拌等方式混合卵黃及脂質成 分物質’且其中總添的脂質成分物質比例需大於卵黃體積 ’由於卵黃中含有乳化劑之成份,因此可於添加脂質成分 201111001 物質後,混合形成油包水型或水包油型(〇/w,觸, 0/W/0,WO/W)介面之單層或多層之乳化液,而於脂質 成分物質詩黃混合過程情可形祕m該微脂粒 中即會包覆有該等專-性抗體,以成本發騎提供之含有 生物可利用性蛋白之乳化組成物。201111001 VI. Description of the Invention: [Technical Field] The present invention relates to an emulsifiable composition which can be directly eaten and a processing method thereof, and more particularly to an edible emulsified composition containing vesicles and a processing method therefor. [Previous technique] The preparation process of the yolk antibody used in the prior art requires the antigen to be injected into the poultry body for one to two months after the vaccination, and the chicken print is collected to prepare the specific immunoglobulin IgY, and then purified. Concentration is made into an immunoglobulin preparation, and the IgY antibody produced by the conventional production process is not only complicated in the process, but often causes the IgY antibody to be inactivated during processing. The vaccine or antibody produced by the above process is easy to be injected into a human or animal body by injection because the protein contained therein is easily denatured by temperature, organic solvent, and unfavorable value. Avoid vaccines or vaccines due to the degenerative reaction produced by oral administration into the digestive tract. The vaccine or antibody is used to protect them from the digestive juice of the organism. It is processed into chemical and organic solutions to make microcapsules. It is also often the case that the active protein in an antibody or vaccine is inactivated by the influence of an organic solution. In summary, the traditional vaccine or antibody administration method and the lack of the above-mentioned production capacity need to be improved. SUMMARY OF THE INVENTION 201111001 The product is produced by providing an emulsified group of a younger age, coated with a bioavailable protein, which can be directly provided as a non-staple food, or a food product. A bioavailable product and a method for producing the same, comprising a lipid substance, an emulsifier, and at least one vesicle. The lipid component material occupies 35 to 90 of the composition of Cao Jinshi; the emulsifier occupies 1% to 65% by weight of the composition. The 〇 脂 脂 contains at least one bioavailable protein. The utilization protein is a water soluble protein. By the process of the present invention, the bioavailability protein can be coated in the -lipid granules, and the emulsifier is selected from edible materials, so the present invention can be directly consumed without making the bioavailable The protein is decomposed in gastric acid', thereby allowing the bioavailable protein to be absorbed by the human body by oral administration into the digestive tract. [Embodiment] For the purpose of detailing the composition and manufacturing process of the present invention, the following preferred embodiments are illustrated with the following description: The present invention - a bioavailable protein provided by the preferred embodiment The ritual composition comprises: an emulsifier, a lipid component material and at least a vesicle; the emulsifier occupies 1% to 65% by weight of the composition; the lipid component occupies % of the composition The percentage of weights up to 9〇 201111001; the microlipids contain at least a bioavailability protein, and the composition of the ml contains at least the egg bioavailability protein. Or pg to 5g. Two == silk is not limited to water-in-oil type or oil-in-water type-generated animal two == == one of the specific antibodies generated by the immune-specific antigen ^ ^ group, the emulsifier is selected from the egg yolk of poultry Bei ^ processing ^ yellow powder, aphid-like yellow matter and its processing, that is, yellow powder",,;! The printing of steam and its processing of printed yellow powder, plant seed extract, high-volume coffee f, _ One of the lipid interface materials and a group thereof; the lipid component material is selected from the group consisting of edible oil, baby oil, animal oil, vegetable oil, mineral oil, and the like. The emulsified composition can be used to make food, non-staple food or food conditioning Wei agent, which can be used to feed or to provide nutrients for humans or animals, such as salads, mayonnaise and other edible foods. The emulsified composition can also be used as a skin care product for the body surface. It can be applied to the body surface to absorb the liposome and the bioavailable protein in the body surface such as the skin or the lips. Referring to the first figure, Is the block flow chart of the present invention, mainly revealed A preferred method of making the composition of the present invention comprises the steps of: (a) comprising an emulsifier comprising a predetermined amount of bioavailability protein 201111001 'wherein the mash is selected from the group consisting of poultry The yellow yam and its processed yolk private moths are the yellow matter and the processed yellow powder, the yellow printed matter of the fish and its processing, the plant seed extract, and the high quality , a phospholipid-like "facial active substance and one of the group consisting of; the bioavailable protein is selected from the group consisting of (four) attenuating globulin, human-synthesis peptide, recombinant protein, bio-in vivo egg production Direct immunization (4) The organism produced by the animal can be derived from the specific antibody produced by the immune specific antigen and the group formed by the organism. (b) The lipid component is in the sputum. Between 56 and ,, the mixture of the above steps is added in batches, and at the same time, the lipid component and the mixture are uniformly mixed by a mixing technique such as high pressure cylinder injection, homogenization, and the like; wherein the lipid component (4) is selected from the group consisting of Oil, baby oil, animal oil, vegetable oil, mineral oil and one of the groups formed by the above. According to the above preferred manufacturing method, the operator can be in the actual operation before the poultry (referred to as chicken and duck) Goose) is injected with a specific antigen in the body. After 3 months of immunization, the above-mentioned poultry will produce a specific antibody (Ig) against specific antigen in the blood circulation system, while the poultry body The oocyte forms a yolk-type after accumulating a certain amount of specific antibody, and the yolk containing the specific antibody is taken out, and the yolk is a natural emulsifier and contains a bioavailable protein (specific antibody) The egg yolk is added to the lipid component substance several times, and after each addition of the lipid component substance, the egg yolk and the lipid component substance are mixed by high pressure cylinder injection, homogenization stirring, etc., and the total lipid component content is larger than the egg yolk. Volume 'Because the yolk contains emulsifier, it can be mixed with water-in-water or oil-in-water type after adding the lipid component 201111001 (〇/w, touch, 0/ W/0, WO/W) interface of a single layer or a plurality of layers of emulsion, and in the lipid component substance poetic yellow mixing process can be shaped, the microlipid will be coated with the specific antibody, The emulsified composition containing the bioavailable protein provided by the cost rider.

你邛八貝於貫際運作較佳製作方法時,亦可以下述方 式呈現··使操作人M將選自於動物生成之免疫球蛋白、人 工合成胜肽、重組蛋白、生物體内生蛋白、直接免疫印黃 質生成動物體所產生之生物可利祕蛋白、生物體經由免 疫特異性抗原所生成之·性抗體及其所域之群组直接 =加入i黃内,加以游後,再分多次加人脂質成分物 質’並於母次添加脂f成分物f後以縫_射出、均質 授拌等方歧合,並使總添加之脂y成分㈣的體積大於 印黃之體積’由㈣黃中含有乳化劑之成份,因而可於添 加脂質成分物質後,形成油包水型或水包油型(講, ’ w_)介面之單層或多層之乳化液,而 :=分物質與印黃混合過程中即可形成微脂粒且該 所=I會包覆有該等生物可利用性蛋白,而成本發明 k供之3有生物可_性蛋白之乳化組成物。 =運作較佳製作方法時,村以下述方式呈現:使 、、重组=選自於動物生成之免疫球蛋白、人卫合成胜肽 體,所逢 t物體内生蛋自、直接免疫纟卩黃質生成動物 原所生物可彻性蛋白、生物體經由免疫特異性抗 '、 特異性抗體及其驗叙群組錢添加入大豆 201111001 卵磷脂中,並於添加後加以攪拌,再分多次加入脂質成分 物質,並於每次添加脂質成分物質後以高壓唧筒射出、均 質攪拌等方式混合,而其中總添加的脂質成分物質體積需 大於大豆印磷脂之體積,由於大豆纟構脂中含有乳化劑之 成份’因而可於添加脂質成分物質後,形成油包水型或水 包油型(O/W,W/0,o/w/o,W/0/W)介面之單層或多 層之乳化液,而於脂質成分物質與大豆卵磷脂混合過程中 即可形成微脂粒,且該微脂粒中即會包覆有該等生物可利 用性蛋白,而成本發明所提供之含有生物可利用性蛋白之 乳化組成物。 惟以上所述者’僅為本發明之較佳實施例及製造流程 而已’當不能以此限定本發明實施之範s,即大凡依本發 明申請專鄉1|及_說_容所作之簡單的等效變化與 修飾’皆仍屬本發明專利涵蓋之範圍内。 【圖式簡單說明】 第一圖係為本發明之方塊流程圖,主要揭示本發 製造流程。 【主要元件符號說明】When you are working on a better method, you can also present it in the following way: · The operator M will be selected from animal-derived immunoglobulins, synthetic peptides, recombinant proteins, and bio-generated proteins. Directly immunizing the yellow-yellow animal body to produce the bio-visible protein, the organism's sexual antibodies generated by the immune-specific antigen and its group directly = add i yellow, after swimming, and then Adding the lipid component material multiple times and adding the fat f component f after the mother, and then dispersing with the seam_emission, homogenizing and mixing, and making the total added fat y component (4) larger than the volume of the printed yellow' (4) Yellow contains an emulsifier, so that after adding a lipid component, it can form a single-layer or multi-layer emulsion of a water-in-oil or oil-in-water type (say, 'w_) interface, and: = sub-substance and printing The liposome can be formed during the yellow mixing process and the ?I will be coated with the bioavailable protein, and the invention may be provided with an emulsified composition of the bio-protein. = When the operation method is better, the village is presented in the following way: make, recombination = immunoglobulin selected from animal, human and synthetic peptide, and raw egg in the t object, direct immunization The bio-obtainable protein and organism of the plastid-producing animal are added to the soybean 201111001 lecithin via an immunospecific anti-specific antibody, and the specificity of the antibody, and are stirred after the addition, and then added in multiple times. The lipid component substance is mixed with a high pressure cylinder after each addition of the lipid component substance, and is uniformly stirred, and the total amount of the lipid component substance to be added is larger than the volume of the soybean printing phospholipid, and the soybean mash has an emulsifier. The component ' can thus form a single layer or multiple layers of a water-in-oil or oil-in-water (O/W, W/0, o/w/o, W/0/W) interface after the addition of the lipid component material. An emulsion, which forms a liposome during the mixing of the lipid component with the soybean lecithin, and the microlipid is coated with the bioavailable protein, and the bioavailable protein provided by the invention Use of sex eggs White emulsified composition. However, the above description is only for the preferred embodiment of the present invention and the manufacturing process, and the invention cannot be limited thereto, that is, the simple application of the invention 1| and _say_容Equivalent variations and modifications are still within the scope of the invention. BRIEF DESCRIPTION OF THE DRAWINGS The first figure is a block flow diagram of the present invention, which mainly discloses the manufacturing process of the present invention. [Main component symbol description]

Claims (1)

201111001 七、 申請專利範圍: 1· 一種含有生物可利用性蛋白之导 物中包含有: Ub組成物,該組成 重=’該脂質成分物質佔有該組成物之 一乳化劑, 百分比,以及 s亥乳化劑佔有該組成物之10至65的重量201111001 VII. Patent application scope: 1. A material containing bioavailable protein contains: Ub composition, the composition weight = 'the lipid component material occupies one of the components of the emulsifier, percentage, and shai The emulsifier occupies 10 to 65 parts of the composition 至少一微脂粒 利用性蛋白。 且§亥等微脂粒中包含有至少一生物可 .依據申%專利範圍第1項所述之組成物,其中:該 生物可利用性蛋白係為一水溶性蛋白。 3.依據申叫專利範圍帛2項所述之組成物,其中: l〇〇ml之該組成物巾至少含有—分子之該生物可利用性蛋 4·依據申請專利範圍第3項所述之組成物其中:該 ❿ 生物可利用性蛋白係選自由動物免疫球蛋白、人^合成^ 肽、重組蛋白、生物體内生蛋白、直接免疫印黃質生成動 物體所產生之生物可利用性蛋白、生物體經由免疫特異性 抗原所生成之特異性抗體及其所組成的群組之一。 5. 依攄申請專利範圍第丨項所述之組成物,其中:該 乳化劑係選自由高量磷脂質、類磷脂質之介面活性物質及 其所組成的群組之一。 6. 依據申請專利範圍第丨項所述之組成物,其中:該 乳化劑係選自由家禽類、爬蟲類、魚類等之卵黃質及其加 201111001 工之卵黃粉所組成的群組之一。 7.依據申請專利範圍第1項所述之組成物,其中:該 乳化劑係為植物種子萃取物。 8·依據申請專利範圍第1項所述之組成物,其中:該 脂質成分物質係選自由食用油、嬰兒油、動物性油、植物 性油、礦物油及其所組成的群組之一。 9. 種應用請求項1之組成物所製成之食品其能夠 藉進食或是飲用為人類或是動物提供養分。 10. 種應用請求項1之組成物所製成之身體表面之 保養ασ,其旎夠藉由塗抹於身體表面使人體吸收微脂粒及 其中之生物可利用性蛋白。 11. 種如请求項1之組合物的製造方法,其係包括 下列步驟: ⑷使一礼化劑中含有一預定量之生物可利用性蛋白 (b)將脂質成分物質分批加人上述步驟之混合物中, 並同時均自混合刻旨質成分物質與該混合物。 12. 依據申請專利範圍第u項所述之製造方法,其中 步驟(b)係於0 C至56°C的溫度下操作。 13. 依據申請專利範圍第u項所述之製造方法,其中 步驟⑷之乳化劑係選自由家禽類之卵黃質及其加工之印 黃粉、爬蟲轉黃質及其加工之㈣粉、魚類之卵黃質及 其加工之卵黃粉所組成的群組之一。 14. 依射請專利範圍第u項所述之麟方法其中 201111001 _⑻之乳化_為植物種子萃取物。 15·依據中請專利範園第u項所述之製造方法,其令 步驟⑻之乳化劑係選自由高量鱗脂質、類鱗脂質之介面 活性物質及其所組成的群組之一。 16. 依據巾請專利範圍第u項所述之製造方法,其中 步驟⑻之生物可彻性蛋自係選自㈣物免疫球蛋白、 人工合成胜肽、重組蛋自、生物體内生蛋自、直接免疫印 黃質生成動物體所產生之生物可顧性蛋自、生物體經由 免疫特異性抗原所生成之特異性抗體及其所組成的群組之 ·"—* 〇 17. 依據申請專利範圍第n項所述之製造方法其中 步驟(b)之混合方式係選自由高壓哪筒射出、 其所組成的群組之一。 18. 依據申請專利範圍第η項所述之製造方法,其 中步驟(b)之脂質成份物質係選自由食用油、嬰兒油、動 物性油、植物性油、礦物油及其所組成的群組之一:At least one liposome utilization protein. And the composition of the first aspect of the invention, wherein the bioavailable protein is a water-soluble protein. 3. The composition according to claim 2, wherein: 〇〇ml of the composition towel contains at least - the bioavailability of the egg. 4. According to the scope of claim 3 The composition: wherein the bioavailable protein is selected from the group consisting of an animal immunoglobulin, a human synthetic peptide, a recombinant protein, an in vivo protein, and a bioavailable protein produced by directly immunizing a yellow-yellow animal. The specific antibody produced by the organism via an immunospecific antigen and one of the groups formed thereof. 5. The composition of claim 2, wherein the emulsifier is selected from the group consisting of a high amount of phospholipids, a phospholipid-like interface active substance, and a group thereof. 6. The composition according to claim 2, wherein the emulsifier is selected from the group consisting of yolks of poultry, reptiles, fish, etc., and yolk powder of 201111001. 7. The composition of claim 1, wherein the emulsifier is a plant seed extract. 8. The composition according to claim 1, wherein the lipid component is selected from the group consisting of edible oils, baby oils, animal oils, vegetable oils, mineral oils, and the like. 9. A food made from the composition of claim 1 which is capable of providing nutrients to humans or animals by eating or drinking. 10. The maintenance of the body surface, ασ, produced by the composition of claim 1 which is sufficient for the body to absorb the liposome and the bioavailable protein thereof by applying to the surface of the body. 11. A method of producing a composition according to claim 1, which comprises the steps of: (4) adding a predetermined amount of bioavailability protein (b) to the liturgical agent to add the lipid component substance in batches to the above steps. In the mixture, and at the same time, the mixture of the components and the mixture are mixed. 12. The manufacturing method according to claim 5, wherein the step (b) is operated at a temperature of from 0 C to 56 °C. 13. The manufacturing method according to claim 5, wherein the emulsifier of the step (4) is selected from the group consisting of yolk of poultry and processed yellow powder, reptile to yellow and processed (four) powder, fish One of the groups consisting of yolk and its processed egg yolk powder. 14. According to the patent, the lining method described in item u of the patent scope 201111001 _(8) is a plant seed extract. 15. The manufacturing method according to the above-mentioned patent application, wherein the emulsifier of the step (8) is selected from the group consisting of a high amount of squamous lipids, a scaly lipid-based interface active substance, and a group thereof. 16. The method according to the invention of claim 5, wherein the bio-degradable egg of step (8) is selected from the group consisting of (four) immunoglobulin, synthetic peptide, recombinant egg, and living organism. Directly immunization of the yellow-yellow animal body produced by the organism can be taken from the egg, the specific antibody produced by the organism via the immune-specific antigen and the group formed by the "-* 〇 17. According to the application The manufacturing method according to item n of the patent scope, wherein the mixing method of the step (b) is one selected from the group consisting of high pressure which is emitted. 18. The manufacturing method according to claim n, wherein the lipid component of step (b) is selected from the group consisting of edible oil, baby oil, animal oil, vegetable oil, mineral oil, and the like. one:
TW98132605A 2009-09-25 2009-09-25 Emulsification composition containing bio-available egg-albumin and its preparation manner TW201111001A (en)

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