TW201033370A - Adipate (ester or thioester) synthesis - Google Patents

Abstract

The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a numer of methods for preparing an intermediate for said or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

Description

201033370 VI. INSTRUCTIONS: [PRIMARY FORMS] STATEMENT OF THE INVENTION The present invention relates to a process for preparing adipic acid or sulphuric acid. The present invention is directed to a method for preparing adipic acid from the brewed or sulphuric acid. Further, the present invention provides various methods for preparing the intermediate of the brewed or sulphuric acid. Further, the present invention relates to a method for preparing 6-aminocaproic acid (6-ACA), a method for preparing 5-methylvaleric acid, and a method for preparing caprolactam . Furthermore, the invention relates to a host cell for use in a method according to the invention. 4 [Prior Art; j 4 Background of the Invention Adipic acid (hexane diacid) can be used for the preparation of polyamines and the like. In addition, esters of adipic acid can be used in plasticizers, lubricants, solvents and various polyamine phthalate resins. Other uses of adipic acid are food acidulants, adhesives, insecticides, gems and dyeing applications. Known preparation methods include oxidation of cyclohexanol or cyclohexanone with nitric acid or a mixture thereof (KA oil). Caprolactam is an internal guanamine which can also be used in the manufacture of polyamidoamines such as nylon-6 or caprolactam-lauric acid amide copolymer (nylon _612). A variety of ways to prepare caprolactam from bulk chemicals are known in the art, including the preparation of caprolactam from cyclohexanone, toluene, cyclohexanol, stupid or cyclohexane. Intermediate compounds for the preparation of adipic acid or caprolactam such as cyclohexanol, cyclohexanone or phenol are typically derived from mineral oil, in view of the increased demand for materials prepared using better green resiliency techniques, A method is provided, 3 201033370 wherein adipic acid or caprolactam is intermediate between intermediate compounds obtainable from biorenewable sources or at least biochemically convertible to adipic acid or caprolactam Prepared as a compound. No. 5,487'987, which describes a process for the preparation of adipic acid in which bacterial cells are used, in which the carbon source is converted to 3 dehydroquinone by biosynthesis of bacterial cells or aromatic amino acid biosynthesis. Oxalic acid is used to produce cis-hexadiene diacid by biocatalytic conversion of 3_dehydrohumulic acid. Subsequently, the cis, cis-hexa-monoacid is chemically reduced (using a catalyst) to produce adipic acid. As such, the last step requires chemical catalysis. The inventors further expect that the aromatic intermediate formed by the bacterial cells is toxic to the cells and may require a low concentration in vivo and in cell culture. It is known to prepare caprolactam from 6-aminocaproic acid (6-ACA), as described, for example, in US-A 6,194,572. Biochemical synthesis of 6-ACA via conversion of 6-amine hex-2-enoic acid (6-AHEA) in the presence of an enzyme having a,yS-enoate reductase activity as disclosed in WO 2005/068643 . 6-AHEA can be prepared from an aminic acid, for example, by biochemical or by purification. Although the method 6-ACA disclosed in WO 2005/068643 can be prepared by reduction of 6-AHEA, the inventors have found that under reducing reaction conditions, 6-AHEA may spontaneously and irreversibly cyclize to form an undesired By-products, notable for the 5-/5-proline acid. Cyclization becomes a bottleneck in the manufacture of 6-ACA, resulting in considerable loss of yield. SUMMARY OF THE INVENTION An object of the present invention is to provide a dragon for the preparation of an intermediate compound which can be used in the preparation of polydecylamine 201033370 and adipic acid or caprolactam or for adipic acid or caprolactam. Method, financial method can be used as an alternative to known methods. A further object is to provide a novel method that overcomes one or more of the aforementioned disadvantages. One or more objects that can be solved in accordance with the present invention will be described hereinafter.

The inventors have made it possible to prepare adipic acid (or its ester or thioester) from succinic acid (or its ester or thioester). In particular, the inventors have concluded that adipic acid can be prepared from a series of specific reactions, such as a reverse 3-oxidation reaction in living cells and biosynthesis of fatty acids, from succinic acid (thio) esters and acetic acid (thio) esters ( The thio) ester 'as shown in Fig. 2, "here, each R represents a reactive group (assisted reaction), for example, as will be described later. Each X represents S or Ο, respectively. ED/EDH2 indicates an oxidized/reduced electron donor such as NAD/NADH, NADP/N ADPH, FAD/FADH2, or oxidized ferredoxin/reduced ferredoxin. The actual transfer of electrons can occur directly, or via an intermediate electron carrier such as coenzyme or electron transport flavin (ETF) media. Y-NH2 represents an amine-based donor', as will be described later. Thus, the inventors have concluded that adipic acid (or its vinegar or its medicinal preparation) must be prepared in a biocatalytic manner by a reaction cascade starting from succinic acid (or its ester or thioester) and acetic acid (or its ester or thioester). Thioester). Further, the adipic acid can be converted into 5-methylvaleric acid ("5-FVA"' 5-carbenoxalic acid) by biocatalysis, which is an intermediate in the preparation of 6-ACA, and is used for the transformation. A specific biocatalyst can be used. Thus, the present invention relates to a process for preparing an adipate or a thioester from butyric acid vinegar or a thioester by a plurality of reactions, wherein at least 5 of the 201033370 reactions are catalyzed by a biocatalyst. In particular, the present invention relates to a process for the preparation of adipate or adipic acid sulphuric acid comprising 2,3-dehydroabiedioic acid in the presence of a biocatalyst (^^8 (:name: 5_carboxy_) 2-pentanoic acid) or 2,3-dehydrohexanedicarboxylic acid thioester is converted to adipate or thioester. [Embodiment; J. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The carboxylic acid or carboxy group is described herein. Acidates such as 6_ACA, other amino acids, 5-FVA, adipic acid/adipate, succinic acid/succinate, acetic acid/acetate' unless otherwise specified, these terms are meant to include protons. a carboxylic acid (free state acid), a corresponding carboxylate (its conjugate base), and a salt thereof. When an amino acid is recited herein, for example, the term is included in its zwitterionic form. An amino acid (in which the amine group is in a protonated form and the carboxylic acid group is in a deprotonated form), wherein the amine group is protonated and the carboxyl group is in a neutral form, and the intermediate group thereof is in a neutral form And a carboxylic acid group in the form of a protonated amino acid and a salt thereof. When referring to an ester or thioester of a carboxylic acid such as an adipate or a thioester or an acetate When sulphuric acid and succinic acid are used, these terms include * any active group', especially any biologically active group including coenzyme A (also known as CoA), scalar broadening, its binding surface group or peptide Protein production (ACP or POO, N-Ethyl-cysteine, methyl thioglycolate, methyl decanoate, ethyl, propionate, methyl decanoate, respectively) , methyl _ Wei Ding Ding _, 巯 叫 called vinegar and other vinegar or sulphur slag that can provide the same or similar functions. In the case of using living cells as a biocatalyst 201033370 under 'Ss or & Sa Sa especially for CoA Biocatalysts are manufactured or derived from organisms that can also be used to catalyze the appropriate enzymes for the reaction. CoA-binding enzymes and CoA-transfers have been identified in a variety of organisms and can provide the desired activated ester or thioester. The preparation of adipic acid vinegar or sulphuric acid from vinegar or sulphuric acid specifically includes the following reaction steps (the number between the brackets also corresponds to the number in Fig. 1): (1) 1⁄4 for succinic acid or sulphuric acid and the series Or thioacetic acid is reacted with acetic acid vinegar or thioester to form 3-keto adipate or thioester (2) deuterating the 3-keto adipate or the ketone group of the thioester to form a 3-diester or thioester; (3) the 3-hydroxyadipate or thioester Dehydrogenating to form 2 3 —dehydroadipate or thioester; and (4) hydrogenating the CC double bond of the 2,3-dehydroadipate or thioester to form adipic acid vinegar or Sulfur vinegar. The present invention is also directed to a process suitable for the preparation of intermediate compounds suitable for use in the process for the formation of adipic acid, comprising one of the reaction steps 1-4 in the presence of a biocatalyst which catalyzes such a reaction step or In one embodiment, the adipate or thioester is converted to 5-FVA (5). If desired, the adipate or thioester can be converted to adipic acid. Or a thioester bond to achieve (7) 'by forming adipic acid; or by a transfer reaction, wherein the "alcohol" moiety or the "thiol" moiety (such as C〇A) is derived from the adipate or thioester Transfer to an acid different from adipic acid, thereby forming adipic acid and an acid (sulfur) S different from adipic acid (7). If succinic acid or acetic acid is used as the different acid, the advantage of this reaction is that the alcohol moiety or the thiol moiety 7 201033370 such as C〇A can be recovered, for example, hexamethylene-CoA+ succinate or acetate can be converted. (usually in the presence of a CoA transferase) to form butadiene 〇c〇A or ethane-CoA+adipic acid. Succinic acid _c〇A or acetamidine _c〇a can then be used as the starting compound for the process of the invention. Adipic acid (or an ester or thioester thereof) can be converted, for example, to 5-FVA (8). In one embodiment, the 5-FVA obtained by the process of the invention is converted to 6-ACA (6). The 6-ACA can then be converted to caprolactam, for example, by methods known in the art. In yet another embodiment, the adipic acid or caprolactam obtained in accordance with the process of the present invention is used in the preparation of polyamine. Words such as "a" as used herein are defined as "at least one" unless otherwise specified. When a noun (for example, a compound, an additive, etc.) is recited in the singular, the plural also includes the plural. When referring to compounds in which several isomers are present (e.g., cis and trans isomers, R and S enantiomers), in principle, such compounds include all pairs of such compounds which are particularly useful in the methods of the invention. Opposites, diastereomers and cis/trans isomers. When the enzyme is described in reference to the enzyme class (EC) in parentheses, the enzyme class is classified or categorizable based on the enzyme nomenclature provided by the International Federation of Biochemistry and Molecular Biology Committee (NC-IUBMB). For naming, please refer to http://www.chem.qmul.ac.uk/iubmb/enzyme{. Other suitable enzymes that have not yet been classified as specific classes but can be classified as such are also included herein. When referring to a protein with an access number here, unless otherwise specified, no 201033370 This number is specifically used to refer to March 2! ! The day has a protein that appears in the sequence of the service. The term "homolog" is used herein specifically for at least 3%, preferably at least 40%, more preferably at least 6%, more preferably at least 65%, more preferably at least 70%, and even more preferably at least 75%. More preferably at least 8 G%, especially at least 85%, more particularly at least 90%, at least 91%, at least 92%, at least 93%, at least 舛%, at least 95%, at least 96%, at least 97%, at least 98% or At least 99% of the polynucleotides of the same sequence or multiple peptides. The term homologue also refers to a nucleic acid sequence (polynucleotide sequence) encoding the same multi-peptide sequence, which differs from other nucleic acid sequences due to the degeneracy of the genetic code. Sequence identity or similarity is defined herein as determining the relationship between two or more multi-peptide sequences or two or more nucleic acid sequences by comparing the two sequences. Generally, sequence identity or similarity compares the full length of the sequence, but it is also possible to compare only the portions of the sequence that are calibrated to each other. In the art world, "identity" or "similarity" also refers to the degree of matching between sequences, the sequence correlation between multiple peptide sequences or nucleic acid sequences, as the case may be. Preferably, the method of determining the degree of identity or similarity is designed to achieve the maximum degree of matching between the sequences tested. In the context of the present invention, preferred computer programs for determining the degree of similarity and similarity between two sequences include BLASTP and BLASTN (Altschul, SF et al, J. Mol. Biol. 1990, 215, 403-410' by NCBI and Other sources are available from the job (BLAST Handbook, Altschul, S. et al., NCBI NLM NIH Basta, Maryland 20894)). The preferred parameters for comparison of multi-peptide sequences using BLASTP are gap opening 10.0, gap extension 0.5, and Blosum 62 matrix. The preferred parameters for nucleic acid sequence comparison using BLASTN are sequence opening 1〇.〇, sequence 9 201033370 extension 0.5, DNA full matrix (DNA identity matrix). In the process of the invention, a biocatalyst is used, in other words, at least one of the reaction steps of the process is catalyzed by a biological material or biological moiety derived from a biological source such as an organism or a biomolecule derived from an organism. Biocatalysts in particular comprise one or more enzymes. The biocatalyst can be used in any form. In one embodiment, one or more enzymes isolated from the natural environment (from the organism from which the enzyme is produced) are used, such as in solution, emulsion, dispersion, lyophilized cells (suspension), lysate, or brake The form of the body is used. The use of an enzyme isolated from the organism from which it is derived is particularly useful because the (4) increase in the reaction conditions is adjusted so that the equilibrium of the reaction moves toward the desired end. In one embodiment, the one or more enzymes form part of a living organism, such as a living whole cell. The enzyme can perform catalytic functions inside the cell. It is also possible that the enzyme can be secreted into the medium in which the cells are present. The living cells can be growing cells, resting resting cells or dormant cells (e.g., spores) or cells in stationary phase. Enzymes that form part of the permeabilizable cells (i.e., become precursors to the permeable matrix or enzyme substrate) can also be used. The biocatalyst used in the process of the invention is in principle any organism or may be derived or derived from any organism. The organism can be a eukaryote, a prokaryote or an archaea. The particular organism may be selected from the group consisting of animals (including humans), plants, bacteria 'archaea, yeast and fungi. Suitable bacteria are particularly selected from the group consisting of Absidia, AChrom〇bacter, Acinetobacter, Agr〇bacj; erium. Aeromonas 201033370 Genus, Alcaligenes, Arthrobacter, Arzoarcus, Azomonas, Azospirillum ), Azotobacter, Bacillus, Beijerinckia, Bradyrhizobium, Burkholderia, Byssochlamys, Citrobacter (Citrobacter), Clostridium, Comamonas, Corynebacterium, Deinococcus, Escherichia, Enterobacter , Flavobacterium, Fusobacterium, Gossypium, Klebsiella, Lactobacillus, Listeria, Megasphaera, Micrococcus, Mycobacterium, Norcadia, Porphyromonas, propionibacterium, False Pseudomonas, Ralstonia, Rhizobium, Rhodopseudomonas, Rhodospirillum, Rodococcus, Listeria, Shewanella, Streptomycetes, Xanthomonas, Xylella, Yersinia, Treponema Treponema), Vibrio, Streptococcus, Lactococcus, Zymomonas, Staphylococcus, Salmonella, Brucella Genus (Brucella), Microscilla. 11 201033370 Suitable eukaryotes may in particular be selected from the group consisting of fungi; multicellular animals; Viridiplantae (especially Arabidopsis and Chlamydomonadales; Diplomonads) (Special Giardiinae; Entamoebidae (especially Entaboeba); Euglenozoa (especially Euglena; Pelobiontida (especially Progesta (Mastigamoeba); and Alveolata (especially Cryptosporidium). Suitable fungi include, in particular, fungi and yeast selected from the group consisting of: Rhizopus, red Neurospora, Penicillium, Aspergillus, piromyces, Trichosporon, Candida 'Hansenula , Kluyveromyces, Saccharomyces, Rhodotorula, Schizosaccharomyces, Yarrowja (such as Yarrowia lypolyt) Ica)) Suitable multicellular animals include, in particular, multicellular animals selected from the group consisting of mammals (including humans), more particularly from the family Leporidae, Muridae, Suidae, Bovidae, hominidae. Biocatalysts can be derived from any part of a multicellular organism such as the liver, pancreas, brain, kidney, heart or other organs. Suitable multicellular animals are also special Including Caenorhabditis and Drosophila. The organisms which are particularly useful for providing a suitable biocatalyst for a particular reaction step when describing a particular reaction step of the process of the invention are described below. It will be apparent to those skilled in the art that it can be used. A natural biocatalyst (wild type) or a mutant of a natural biocatalyst with an appropriate activity according to the method of 201033370. The properties of the natural biocatalyst can be improved by biotechnological improvements known to those skilled in the art, such as molecular evolution or theoretical design. A mutant strain of a wild-type biocatalyst can be, for example, a mutation-generating technique known to those skilled in the art. (random mutation occurrence, position-directed mutation generation, directed evolution, genetic recombination, etc.) can be produced by modifying the coding DNA of an organism which can function as a biocatalyst or which can produce a biocatalytic moiety such as an enzyme. Specifically, the DNA is modified such that the DNA encodes at least one amino acid enzyme different from the wild-type enzyme, thus encoding the wild type containing one or more amino acid substitutions, censoring and/or insertions, or allowing mutations The strain combines the expression of two or more parental enzyme sequences' or by performing such modified Dna on appropriate (host) cells. The latter can be achieved by methods known to those skilled in the art, such as codon optimization or codon pair optimization methods, for example based on the method described in WO 2008/000632. Mutant biocatalysts have improved properties, for example, improved properties for one or more of the following phases: selectivity to enzyme matrix, activity, stability, solvent tolerance, pH profile data, temperature profile data, enzyme matrix profile Data, susceptibility to inhibition, cofactor utilization, and enzyme matrix affinity. Mutants with improved properties can be identified based on methods known to those skilled in the art, such as appropriate high throughput screening or selection methods. The enzyme substrate specificity of the enzyme acting on the alkyl or alkyl ester or thioester can be modified. Molecular evolution can be utilized to form diversity, followed by screening of desired mutants and/or rationally engineered enzyme matrix binding pockets. The enzyme matrix specific modification technique of the enzyme used in the method of the present invention can be based on the method known to those skilled in the art 13 201033370. For example, the use of structural and sequence information to rationally engineer specific mutants has been used to modify the enzyme matrix specificity of the thiol transferase site 4 derived from erythromycin polyketide synthase. Other brewing bases. It has been shown that modification of the suggested enzyme matrix binding site results in a modified binding pocket that can be combined with other enzyme matrices to achieve different product ratios (Reeves, C.D.; Murli, S.; Ashley, G.W.;

Piagentini, Μ. ; Hutchinson, C.R. ; McDaniel, R. Biochemistry 2001' 40(51), 15464-15470). Both the theoretical design method and the molecular evolution method have been used to change the enzyme matrix specificity of the biocatalyst BM3, resulting in the replacement of the natural enzyme matrix of the medium chain fatty acid (such as myristic acid) or the medium chain fatty acid natural enzyme matrix, and a large amount of oxidizable. Mutants of various different alkenes, cycloolefins, arylenes and heteroarylenes (Peters, MW; Meinhold, Ρ· ; Glieder, A. ; Arnold, FH American Chemical Society Journal 2003, 125(44), 13442 -13450 ; Appel, D. ; Lutz-Wahl, s. ; Fischer, P.;

Schwaneberg, U.; Schmid, R. D. Biotechnology Journal 2001, 88(2), 167-171 and references therein). When referring to a biocatalyst derived from a particular source, particularly an enzyme, it is specifically meant to include a recombinant biocatalyst, particularly an enzyme, derived from a first organism but actually in a (genetically modified) second organism. The biocatalyst from the first organism is in particular an enzyme. Preparation of 3-ketoadipate (ester or thioester) ("Reaction 1") In the examples of the present invention, 3-ketoadipate (ester or thioester) is prepared from succinate and acetate The succinate and acetate actually have an activating group, and in particular an ester or a thioester can be obtained to assist the reaction. 201033370 朗i adipic acid (vinegar or thioester) can be achieved by biocatalytic or chemical means by a Claisen condensation reaction in which acetate or sulfur S is coupled with a succinate or thioester. . In a preferred process of the invention, the preparation comprises the biocatalytic reaction in the presence of a biocatalyst capable of catalyzing sulfhydryl transfer. An enzyme having such catalytic activity can thus be referred to as a transductase. In a preferred method, the thiol transfer is carried out between two thiol esters or oxime esters. It is better to turn it into 醯C_A) and Ding 醯·〇)Α. Preferably, the catalyst is selective for the sulphuric acid. The biocatalyst comprises in particular an enzyme which can carry out thiol transfer, which is selected from the group consisting of a transductase (EC 2.3.1), preferably selected from 2> ii-CoA. ^ ^- C〇AC-6 ai#^a^(EC 2.3.1.9) ^-CoA : 乙醯-CoAC-转酿崎2 3116) and 丁二酿_c〇A :乙酿_c〇a C-丁The diterpene transferase (EC such as (10), also referred to as _ _ _ _ _ thiolase) grouped. In the case of Wei Ming Wei (Kyoto Encyclopedia of Genes and Genomes), plant oxidation, fatty acid biosynthesis, polyketide biosynthesis, or butyrate metabolism. The transductase is particularly a reconstituted enzyme selected from the group consisting of an organism consisting of archaea, bacteria and eukaryotes. In particular, the enzyme may be derived from a microorganism which decomposes an organic compound comprising an aromatic ring structure or a cycloaliphatic ring structure, particularly a 5, 6, or 7 member ring structure. The organic compound may optionally contain one or more heteroatoms or a moiety as a substituent or substituent in the ring. For example, the organic moiety may be an aromatic compound 'particularly an aromatic compound containing a 6-membered ring. In particular, the aromatic 15 201033370 family compound may be selected from the group consisting of stupid acetic acid _, benzophenone _, catechu, acid vinegar and gentisic acid. The organic compound may be a cycloaliphatic compound, particularly a bad alcohol such as cyclohexanol, a ring (tetra) such as cyclohexanone, or a ring-burning such as a ring. The organic compound can be an internal guanamine such as caprolactam. In one embodiment, the enzyme is derived from an organism which decomposes ditoponic acid (usually c6-c1 oxime), particularly a linear saturated dicarboxylic acid such as adipic acid. In yet another embodiment, the enzyme is derived from a synthesizable 3 adipic acid as part of a secondary metabolite (eg, a mannomycin)

_〇n〇mycin)) is preferred as 'from Streptomyces rimosus, especially from Actinomyces (Actin〇myc is called from other Actinobacteria (Actinobacteda) Or other known secondary metabolite c. Preferred microorganisms for providing a biocatalyst capable of catalyzing the preparation of 3-ketoadipate (ester or thioester) further include Acinetobacter (particularly Acinetobacter) ADP1 and A calc〇aceticus, soil genus (especially A. tumefaciens), Alcaligenes (especially for the bacterium lineage D2) And A ® eutmphus, Arthrobacter, Denitrification (A evansii), Azotobacter, Azotobacter, Bacillus (especially B. halodurans, Phytophthora, genus Rhizobium, Burkholder, Clostridium (especially Ckluyveri, acetobutyric acid) Acetobutylicum, c beijerinckii, crown Cyanobacteria, Corynebacterium (especially C. glutamicum and C. aurantiacum), large intestines 16 201033370 (E.coli), Enterobacter, Flavobacterium Genus, Megabacterium (particularly M. elsdenii), Nocardia, Pseudomonas (especially P. putida, Pseudomonas aeruginosa (p Aeruginosa) and Pseudomonas strain B13), Ralstonia (especially R. eutropha), Rhizobium, Rhodopseudomonas (especially Rhodopseudomonas (R. pahmris), Rhodococcus (particularly R erythropolis, R 〇pacus and Rhodococcus sp. RHA1), Fusarium (especially A. niger), Euglena (especially EUglena gracilis), Rhodobacter (especially CraSSa), Penicillium (especially P. chrysogenum) )), Rhodotorula, Saccharomyces, Trichosporon (particularly T Cutaneum). In a particular embodiment, the biocatalyst comprises a sequence ID An enzyme for recognizing an amino acid sequence of any of 113 or its homologs. 3_Preparation of hexamethylene dioxime (ester or thioester) ("Reaction 2") In one embodiment, 3-hydroxy hexane The acid (ester or thioester) is prepared from 3-ketoadipate (ester or thioester). Usually, 3-ketoadipate is provided as an active group as described above. In principle 3-hydroxyadipate (ester or thioester) can be prepared chemically, for example via selective hydrogenation of a 3-keto group in 3-ketoadipate (ester or thioester). Such a reaction is particularly carried out in the presence of a biocatalyst which catalyzes the reaction step, in particular a biocatalyst which catalyzes the reduction of a ketone group, more particularly a carbonyl group to a hydroxyl group. In a specific embodiment, the 3-ketoadipate is present in the form of a thioester 17 201033370 with coenzyme a (hereinafter, the thioester of 3-ketoadipate and coenzyme A will be referred to as 3-ketohexanide -CoA). In a preferred method of the invention, the preparation comprises a biocatalytic reaction in the presence of a biocatalyst which catalyzes the reduction of 3-mercapto (ester or thioester) to 3-oxindole (ester or thioester). An enzyme having such catalytic activity is thus referred to as a 3-branched (ester or thiopurine) dehydrogenation. Therefore, an enzyme having such catalytic activity for 3-hydroxyindole c-A-succinate is referred to as 3-hydroxyindole CoA-dehydrogenase. Preferably, the 3-hydroxyindole CoA-dehydrogenase is selective for the enzyme substrate 3-ketohexanide-CoA. The enzyme which catalyzes the reduction of 3-ketooxime (ester or thioester) to 3-oxindole (ester or thioester) is particularly selected from the group consisting of dehydrogenase (E C 1.1.1), preferably

Is selected from the group consisting of 3-hydroxyindole_c〇A dehydrogenase (EC 1.1.1.35 and EC 1.1.1.36), 3-hydroxybutyrene_C〇A dehydrogenase (Ec 1.1.1.157), 3-hydroxyl Geng Erqi

a group consisting of -CoA dehydrogenase (EC 1.1.; ι 259) and long chain 3-hydroxyindole-CoA dehydrogenase (EC 1.1.1.211). These enzymes can use \8::11 or 1^8 1)1)11 as a cofactor. According to the KEGG (Kyoto Gene and Genome Encyclopedia) database, 3-branched-CoA dehydrogenase activity has been described, for example, in fatty acid metabolism, polyketide biosynthesis, polyhydroxyalkanoate metabolism, butyrate metabolism, and aromatic compounds. break down. Specifically, the microorganism can decompose the organic compound (referred to as "Reaction 1") as previously identified, and is particularly an aromatic compound, a cycloaliphatic compound or a dicarboxylic acid. Other preferred organisms for providing a biocatalyst capable of catalyzing the preparation of 3-adipate (S or sulphuric acid) include: immobility (especially for Acinetobacter spp. ADP1 and Acinetobacter aceti (a. Detector 18 201033370 genus (Alcaligenes X is especially for Alcaligenes faecalis 〇 2 and A. emrophus), denitrifying genus (specially denitrifying bacteria), Bacillus (especially for hobby) B hal〇durans, Bordetella (especially for B. pertussis (B卩州), Burkella (special B. pSeud〇mallei and B. xen〇) V〇rans), Corynebacterium (especially for Corynebacterium amyloliquefaciens, Corynebacterium faecalis and C. efficiens), Dinocobacter (especially Radiform Dinodus D, large intestine) Φ bacterium, Klebsiella (K. pneumonia), Pseudomonas (especially Pseudomonas prgenskii and Pseudomonas fluorescens) P. fluorescens)), Rhodopseudomonas (especially R. palustris), Coccus (especially Rhodococcus rhodochrous (R. " erythr〇P〇lis), Rhodococcus erythropolis (R· opacus) and Rhodococcus sp. RHA1), Fusarium (especially A niger) ), Rhodotorula (especially N. crassa), Penicillium (especially Chrysogeimm), Saccharomyces (especially Saccharomyces cerevisiae (s. • used to provide action on 3_ ketone) Suitable organisms of the EC 1.1丄35 enzyme of 醯-C〇A may be obtained from any organism including mammals, particularly selected from the group consisting of European cattle (Bos taums), brown rats (Rattus norvegicus), European pigs (Sus scrofa) and Mammals in a group consisting of human (H〇mo sapiens). Suitable biocatalysts involving (anaerobic) fatty acid synthesis, polyketide biosynthesis or polyhydroxyalkanoic acid metabolism can be obtained from any organism, especially microorganisms. These include: Clostridium (specially Clostridium butyricum and Clostridium clostridium (especially for the genus Euglena), Macrococcal (especially for the genus Erythromycin), and genus Rastis Especially for the genus Rasporia), and the fungus group (z〇〇gi〇a) 19 201033370 (particularly Zoogloea ramigera). In a particular embodiment, the biocatalyst (catalyzed "Reaction 2") comprises - an enzyme comprising, for example, sequence IDs 15-26, 29 or homologs thereof The amino acid sequence identified by either of them. It is specifically contemplated that an enzyme comprising an aminopurine sequence according to sequence ID 26 catalyzes "Reaction 2" and "Reaction 3". Preparation of 2,3-dehydroadipate (ester or thioester) ("Reaction 3") In one embodiment, 2,3-dehydroadipate (5-carboxy-2-pentanoic acid) Or a thioester) is prepared from 3-hydroxyadipate (ester or thioester). Optionally, 2,3-dehydrohexanedioic acid and 3-adipate are coupled to a coenzyme, ACP or other reactive group as described herein. In one embodiment of the invention, 2,3-dehydroabietate can be prepared by chemically converting 3-hydroxyadipate (ester or thioester), for example, in the absence of water in the presence of sulfuric acid in an anhydrous environment ( Ester or thioester). 2,3-Dehydroabietate is prepared in particular from 3-hydroxyadipate using at least one biocatalyst. A preferred biocatalyst is a biocatalyst which catalyzes the dehydration of 3- oxime ester or sulphur to its 2-ene oxime ester or its 2-enyl thioester. In a particular embodiment, the 2,3-dehydroadipate is present as a thioester of coenzyme a (hereinafter 2, the thioester of 3-dehydroabietic acid and coenzyme A will be referred to as 5_绫-2 - Pentenacene-CoA). In a specific embodiment, the biocatalyst catalyzes the 3-hydroxyhexanyl-(: 〇8 dehydrated to 5-carboxy-2-pentenyl oxime-CoA. In particular, the biocatalytic reaction can catalyze the 3-hydroxyindole The (sulfuric acid) vinegar is dehydrated to a 2,3-dehydroabietyl ester biocatalyst in the presence of a biocatalyst. Therefore, the enzyme having such activity is referred to as a 3-mercapto (ester or 琉g) dehydration 20 201033370 enzyme. 3-Hydroxyindole CoA-thioester The enzyme having such catalytic activity is therefore referred to as 3-hydroxyindole-CoA dehydrating enzyme. Preferably, the 3-hydroxyindole-CoA dehydrating enzyme system is for the enzyme substrate 3-hydroxyhexamidine. -CoA is selective.

An enzyme which catalyzes the dehydration of 3-hydroxyindole (ester or thioester) to 2,3-dehydropurine (brewed or sulphate) is especially selected from the group consisting of dehydratase (EC 4.2.1). The best is selected from the group consisting of olefin-CoA hydratase (EC 4.2.1.17), 3-hydroxybutyro-c〇A dehydratase (EC 4.2.1.55) and long-chain olefin-CoA hydratase (EC 4.2). .1.74) Reference

The group formed. According to the KEGG database, 3-oxindole-CoA dehydration activity has been described, for example, in fatty acid metabolism, synthesis of polyketides, or metabolism of butanone acid, and decomposition of aromatic compounds. The 3-hydroxyindole (ester or thioester) dehydrating enzyme may be one selected from the group consisting of archaea, bacteria, and eukaryotes, for example, selected from the group consisting of yeast, fungi, and mammals. 3 oxindole (ester or thioester) dehydratase of the organism.可0 The above-identified decomposable organic compound (refer to "Reaction lj", especially the aromatic compound 'cycloaliphatic compound or dicarboxylic acid microorganism catalyzed by 2,3 · adipic acid (g or (4)) A preferred source of the biocatalyst. Knife-dissolving aroma compounds, cycloaliphatic compounds, or dicarboxylic acids of the genus Acinetobacter spp. (especially the Acinetobacter strains adpi and calcium acetate), and the genus Bacillus (particularly for production) Phytophthora D2), genus Fusarium (especially for black sputum, _ _ genus (especially for the denitrifying bacteria), nitrogen-fixing bacteria, nitrogen-fixing seedlings ^ gΜίά,,, genus Especially for Bacillus haemophilus), Corynebacterium genus Corynebacterium and Corynebacterium baumannii, Escherichia coli, Rhodopseus (especially for the red genus), Penicillium (especially for yellowing) Qing 21 201033370 mildew, pseudo single cell __彳 for #单单状状丝红假单单_ (specially transferred to red material _), red ball = Rhodococcus species RHA1). Do not provide an effect on 3 - a preferred body of the 2117 enzyme comprising a group consisting of a mammal and a microorganism The EC4.2.1.I7 enzyme suitably derived from a mammal is obtained from a group selected from the group consisting of European cattle A, brown and European pigs. EC4.2.1.17 suitably obtained from microorganisms. Aeromonas

(Aer〇m〇naS) (Specially, Aeromonas aeruginosa (A., Clostridium (special clostridium butyric acid), cotton genus (5) (four) coffee ton special American cotton fungus along hlrSUmm)), red A group of genus Spirulina (particularly R. mbnimi) and Rasta genus (_Nutrition ras _). It is also preferably a microbial organism capable of performing (anaerobic) fatty acid biosynthesis. These micro (four) include shuttle g (especially for Clostridium oxysporum and Kluyveromyces cerevisiae), Euglena m. special larvae, giant ball (Specific Escherichia coli), and Saccharomyces (especially Saccharomyces cerevisiae).

Suitable enzymes in particular comprise an amino acid sequence according to any of the sequence IDs 14, 27, 28, 3〇 41, 92 or a homologue thereof. Preparation of adipic acid from 2,3·degassing (vinegar or sulfur) (series or sulfur ("Reaction 4") In one embodiment, adipic acid (g or sulphuric acid) is from 23 Preparation of dehydrogenated hexanyl (s or chelates). The monoacid (ester or thioester) can be prepared chemically from 2,3-dehydroadipate (ester or thioester), for example by C2 <:3 double bond Prepared by selective hydrogenation or prepared by biocatalysis. Usually 2,3-dehydroadipate has an active group, as indicated above 22 201033370 Hexanoic acid (S or sulfur) preferably uses at least one catalyzable a biocatalyst for the hydrogenation of a carbon-carbon double bond of valeric acid (ester or thioester) and prepared from 2,3-dehydroadipate (ester or thioester). In a preferred method of the invention, The preparation comprises a biocatalytic reaction in the presence of a biocatalyst capable of catalyzing the reduction of cis or trans-2-olefin (ester or thioester) to hydrazine (ester or thioester). The biocatalyst can use a range of electron donors' From the electron donor in a group consisting of NADH, NADPH, FADH2, and reduced • iron redox proteins. Electrons can be directly applied by electron donors. Moved to a biocatalyst, or otherwise transferred to a biocatalyst by an electron donor, especially by the so-called electron-transfer yellow protein (ETF) medium. Therefore, the enzyme having such catalytic activity is called 2-ene oxime (ester or thioester). Reductase (ER). Therefore, the 2-olefin oxime-CoA thioester has such a catalytic activity as a 2-olefin oxime-CoA reductase. Preferably, the 2- olefin 醯c〇A reductase The substrate 2,3-dehydrohexamidine-CoA is selective. The enzyme which catalyzes the reduction of 2-ene oxime (ester or thioester) is especially selected from ruthenium oxidoreductase (EC 1.3_1 and EC 1.3. 99) The group consisting of

From the group consisting of enelt-CoA reductase (ec 1.3.1.8, EC 1.3.1.38 and EC 1.3.1.44); selected from the reduction of olefinic 醯 [醯 carrier protein] (: 1.3. 1.9, £0: 1.3.1.1〇 and the group consisting of 1.1.3.39; and selected from Dingshao-CoA dehydrogenase (EC L3.99.2), 醯^ from dehydrogenase (1.3.99.3) And a group consisting of long chain 醯·(::〇Δ dehydrogenase (EC ! 3 99 13). Trans-2-enyl (ester or thioester) reductase activity has been described, for example, according to KEgg Fatty acid metabolism, polyketide synthesis, butyric acid metabolism, and mitochondrial fatty acid biosynthesis. 23 201033370 2-Enyl (ester or thioester) reductase can be obtained or derived from any organism in principle. Special organisms can be selected from bacteria. An archaea or eukaryote, such as a group selected from the group consisting of yeast, fungi, protists, plants, and animals (including humans). In one embodiment, the organism may be selected from the group consisting of: Escherichia coli, Vibrio, Bacillus (especially Bacillus subtilis), Clostridium (especially Kluwer _, Clostridium aceta, Clostridium Clostridium perfringens (C. perfringens) 'Streptococcus (particularly s coelicolor and s avermitilis), Pseudomonas (special Pseudomonas) Pseudomonas and Pseudomonas aeruginosa, Schwannella, Xanthomonas, xylobacter, Yersinia, Treponema (especially T. dentate), gas production list Phytophthora (especially Aeromonas hydrophila), Bradybacterium (particularly Microscilla marina), Megacoccal (especially Megacocci), Dinobacter Genus (especially Radiated Dinoococcus), Yarrowia (especially for the decomposition of A. faecalis) and Eubacterium (especially E. pyruvativorans). In the '2-enoquinone (ester or thioester) reductase, the organism is obtained from an organism selected from the group consisting of the eye worm (particularly Euglena). In one embodiment, '2-ene oxime (ester) Or a thioester) reductase derived from a genus of the genus Saccharomyces (Special Saccharomyces cerevisiae), Kluyveromyces (particularly lactic acid) K. lactis, Schizosacchar〇 myces (specially S. pombe), Candida (c. tropicalis) The organism in the group. 24 201033370 In the examples, the 2-olefin (ester or thioester) reductase is selected from the group consisting of the genus of the genus (specially black bacterium and A. nidulans). And the organism of the genus of the genus Penicillium (specially produced by Huang Qingzheng). In the examples, the '2-thin (S or sulphur) reductase is obtained from an organism selected from the group consisting of genus (especially A. thaliana). In one embodiment, the 2-enyl (ester or thioester) reductase is selected from the group consisting of human, brown rat, European cattle, Cavia sp., Caenorhabditis elegans, and black belly. An organism in a group of Drosophila melanogaster. Suitable enzymes in particular comprise an amino acid sequence according to any of sequence ID 42-67, 94, 96, 98, 100, 105 '107, 1〇9, in, 113 or a homologue thereof' more particularly according to the sequence ID The amino acid sequence of any of 60, 63, 96, 100 or a homologue thereof. Examples of nucleotide sequences encoding appropriate enzymes for catalyzing "Reaction 4" are represented by 2-enyl (ester or thioester) reductases 93, 95, 97, 99, 104, 106, 108, 110 and 112. In a preferred embodiment, the use of ETF in addition to the 2-olefin (ester or thioester) reductase facilitates the activity of the reductase. Such ETFs may be derived or derived from an organism as hereinbefore identified or derived from a reductase. It may in particular be derived or derived from the same organism as the reductase used, more particularly the same organism. A particular ETF comprises the amino acid sequence represented by sequence IDs 102, 103, 115, 116. Sequence IDs 101 and 114 represent nucleotide sequences encoding a particular ETF. Typically such ETFs comprise two subunits (etfA and etfB) encoded by two different genes. Typically used in combination to make active ETF proteins. For example, 25 201033370 may use the following composition: sequence ID 102 and sequence ID 103 or a combination of sequence id 116 and sequence ID 115. Skilled people can choose other suitable ETF compositions known to the art world. In embodiments of the invention, a biocatalyst having a desired activity or enzyme matrix specificity can be modified by methods known in the art, such as by theoretical design or molecular evolution modification to form a catalyst that can be rationally or reasonably selective 2 3-Dehydroabietate (ester or thioester) is converted to a mutant of adipic acid (ester or thioester). It is preferred to use a biocatalyst having a chain length of 6 2-ene-CoA derivative, which is obtained from Clostridium clostridium, European cattle, Euglena, genus genus, Saccharomyces cerevisiae, tropical silk Yeast, human and biocatalysts of P. pyruvate are particularly good. Preparation of adipic acid ("Reaction 7") 乂 According to the present invention, an adipate or a thioester can be used for the preparation of adipic acid by hydrolysis of an ester bond or a thioester bond. It can be achieved chemically, for example in the presence of an acid or a base or by chemical catalysis. In a preferred method of the present invention, a biocatalytic reaction is carried out in the presence of a biocatalyst which is hydrolyzed by a (tetra) (thio) vinegar. The enzyme having catalytic activity can be referred to as a brewing (sulfur) vinegar hydrolase. The enzyme which has such catalytic activity for the brewing of CoA sulphuric acid is therefore referred to as 醯_c〇a hydrolyzed. Preferably, the 醯-CqA hydrolase system _substrate hexamethylene _CqA is selective. The hydrolysis of ruthenium (sulfur) gambling is particularly selected from the group consisting of hydrolyzed plum (EC 3.1.2) 'preferably selected from Yufu hydrolysis hydrolyzate (EC 3.L2.20) ), (10) partial _^ 3 i 2a), long bond fat _ brewing - C 〇 A hydrolase (Qi (1)), Ding siu is very hydrolyzed (four) Ec3j23) 26 201033370 and brewing - [stuffed - carrier - protein] - hydrolysis a group of enzymes (EC 3 12(4). The biocatalyst comprises an enzyme derived from any organism including archaea, bacteria or eukaryotes. The special biocatalyst comprises an enzyme selected from the group consisting of the following: : Escherichia coli, Bmcella (Special Brucella melitensis), Agrobacterium (special nodule soil rod), Rhizoctonia genus, Sinorhizobium (Special Sinorhizobium meliloti, MeSorhiz〇biUm (special Mes〇rhiz〇bium. 1〇tl), Vibrio, Streptomyces (especially Streptomyces coelicolor and A. streptococci), Rhodopseudomonas (especially Rhodopseudomonas palustris), Quality genus, yeah

X-genus, Pseudomonas (special Pseudomonas and Pseudomonas aeruginosa), Schwannella, Shigella, Salmonella, Corynebacterium, Mycobacterium, Hyphium monas (Hyphomonas neptunium) and oleic acid genus. Φ Suitable biocatalysts are particularly found in yeast selected from the group consisting of Saccharomyces (Special Saccharomyces cerevisiae) and Kluyveromyces (Specific Kluyveromyces lactis). Suitable biocatalysts are particularly found in fungi selected from the group consisting of the genus Fusarium (specially, the genus A. fumigatus and the genus) and the genus Penicillium (particularly P. chrysogenum). . In yet another embodiment, the organic system is selected from the group consisting of Arabidopsis (particularly Arabidopsis), Muridae (particularly brown rat, mouse (Mus musculus), bovidae (special European) a group of cattle, sheep, and humans, and a genus of Caenorhabditis (Caen〇rhabditis elegans). In one embodiment of the present invention, Biocatalysts which do not themselves have the desired activity or enzyme matrix specificity can be formed by techniques known in the art, for example by theoretical design or molecular evolution modification, to form mutants which can efficiently convert adipate or thioester to adipic acid. It is preferred to use a biocatalyst having an initial activity with a C4-C8 acid, particularly a bismuth-CoA derivative of a dicarboxylic acid. For example, a mutant strain can be formed based on a sputum-CoA-thioesterase obtained from a mouse (for example, In Seq ID 73). In a particular embodiment of the invention, the preparation procedure comprises a biocatalytic reaction in the presence of a biocatalyst which catalyzes the transfer of an active agent, particularly an ester or a thioester, most particularly CoA. Active It may be referred to as a C〇A transferase. Preferably, the CoA transferase is selective for a dicarboxylic acid-C〇A which is an enzyme substrate for CoA. More preferably, the dicarboxylic acid-CoA is hexamethylene-CoA. Preferably, the CoA system is selective for acetic acid as a CoA-receptor substrate. The enzyme capable of catalyzing the transfer of CoA groups is particularly selected from the group consisting of c〇A transferase (EC 2.8.3), preferably Selected from the bis-acid _c〇A: dicarboxylic acid CoA transferase, adipic acid: butadiene-CoA CoA transferase, 3-minic acid CoA-transferase (EC 2.8.3.5), 3-ketone A group consisting of adipic acid CoA-transferase (EC 2.8.3.6) and acetic acid CoA-transferase (EC 2.8.3.8).

CoA transferases can in principle be derived or derived from any organism. The organism can be a bacterium, an archaea or a eukaryote. In particular, it is preferred that the organism which decomposes the diacid to specifically decompose adipic acid is preferred. 201033370 The organism is particularly selected from the group consisting of the genus of the genus of the genus (specially fixed rods _ s), bacteria in the group: Clostridium (Special Kluyveromyces cerevisiae, (10) 1, Actinobacillus acetate), pseudomonas Genus (especially for Platts (four) or Clostridium sylvestris), sapphire fluorescens Pseudomonas, soil rod (10), alkali-producing genus, arthritis genus & Guardian® solid-air premature genus, Azospirillum, genus of genus, genus Bacillus, pL ^ m, genus, genus, genus, genus, genus, burdock Cyanobacteria,

Rhizobium, Rhodotorula, Rhodococcus, Nocardia, genus, genus, genus, and Roseburia sp. The organism is particularly a yeast or fungus selected from the group consisting of noodles (especially yellow) and genus. Particularly suitable CoA transferases may be derived from or derived from one of the human family (Hominidea), more particularly from humans. An enzyme suitable for use in Reaction 7 specifically comprises an amino acid sequence according to any of the sequences still 68-73, 85, 116, 117, 119-124 or a homolog thereof. The preparation of adipic acid from a thioester is particularly catalyzed by a biocatalyst comprising a ruthenium-CoA hydrolase containing an amino acid sequence according to one of Sequence Engineering 68-73, 117, 119 or a homolog thereof. The preparation of adipic acid from sulphuric acid may in particular be carried out by a biocatalyst comprising a CoA transferase comprising an amino acid sequence according to one of the sequence IDs 85, 121, 122, 123, 124, 125, 126 or a homologue thereof. catalytic.

A CoA transferase can be encoded by a single gene or by more than one gene. For example, several CoA transfer drunks contain two subunits encoded by two different genes. These subunits are commonly used together to allow C〇A to transfer enzyme proteins 29 201033370

With / tongue. For example, the following composition may be used: a combination of sequence d 121 and sequence ID 122 or a combination of sequence ID 125 and sequence id d. 5FVA ("Reaction 5") was prepared from -- or adipic acid sulfur s | In the examples, 5-methylvaleric acid (5-FVA) was prepared from adipate or hexanoic acid. The preparation can be carried out chemically or biocatalytically. * Adipic acid vinegar is specifically coupled to CoA or coupled to other reactive groups as indicated above. In particular, the present invention also provides a method for preparing 5-FVA from adipate or bismuth adipate in the presence of a biocatalyst capable of catalyzing the reduction of oxime ester or sulfonium thioester, particularly for use in the preparation of 5-FVA. The method of preparing 5-FVA by 醯_c〇A thioester. Therefore, an enzyme having such catalytic activity can be referred to as an aldehyde dehydrogenase. Therefore, an enzyme having such a catalytic activity for an oxime ester or a sulfonium ester such as 醯_c〇A thioester can be referred to as a dehydroquinone (acetamidine). Preferably, the biocatalyst comprising aldehyde dehydrogenase (acetamidine) is selective for the enzyme matrix adipate or thiodicarboxylate. The enzyme which catalyzes the reduction of thiol (thio) ester is selected in particular from the group consisting of oxidoreductases (EC 1.2.1), preferably selected from the group consisting of aldehyde dehydrogenase (acetylation) (EC) 1.2.1.10), fat 醯-CoA reductase (EC 1.2.1.42), long-chain fat 醯-CoA reductase (EC 1.2.1.50), butadiene dehydrogenase (EC 1.2.1.57) and succinic semialdehyde A group consisting of dehydrogenase (e.g., see Sohling et al., 1996, JBacteriol. 178: 871-880). The dehydrogenase can in principle be derived or derived from any organism. It is to be understood that the enzyme is obtained from the multi-gene 30 201033370 body source by direct isolation of the encoding nucleic acid and subsequent determination of activity in a non-homologous host, or by sequence homology found in the multi-source gene D N A . The organism can be a bacterium, an archaea or a eukaryote. The particular organism may be selected from bacteria, more particularly selected from the group consisting of Escherichia coli, Clostridium (Special Kluyveromyces, Clostridium cloacae, Clostridium butyricum, C. botylicum, C. tetani, Clostridium perfringens and Clostridium viridis (cn〇Vyi), Porphyromonas gingivalis, Lis Listeria, oleic acid genus (especially Phytophthora fuliginea), Enterococcus, Clostridium, Lactobacillus (especially Lactobacillus lactis), Bacillus (especially for B. thuringiensis), Burkholderia (especially B. thailandensis and B. mallei), Pseudomonas (especially P. sinensis) Monocytogenes), Rhodococcus (particularly Rhodococcus species RHA1) and Salmonella (especially Salmonella typhi). The organism may also be selected from eukaryotes, more particularly from Giardia (especially g. lamblia), Entamoeba. (especially for E. Histolytica), Mastigamoeba balamuthi, Chlamydomonas reinhardtii, Polytomella, Piromyces, Cryptosporidium Genus (Cryptosporidium), and cristospores (Spironucleus barkhanus). Suitable dehydrogenases, in particular, comprising an amino acid sequence according to any of Sequence IDs 74-81, 139-148, or a homolog thereof, in the examples of the present invention, do not themselves have the desired activity or enzyme matrix specificity The biocatalyst can be modified by theoretical methods or molecular evolution to form a mutant capable of converting an adipate or a thioester into a 5-FVA. It is preferred to have a biocatalyst which can degrade aldehyde dehydrogenase activity using a ruthenium-CoA derivative of chain 31 201033370, which includes 4-8, including but not limited to, from Kluyveromyces cerevisiae (sequence ID 74) or gums. Succinic acid semialdehyde dehydrogenase (acetylation) of Porphyromonas gingivalis (sequence ID 75) and acetal butyric acid acetal (sequence ID 80, 81) or P. stipitis (sequence ID) 79) A biocatalyst of butyraldehyde dehydrogenase (acetamidine) is preferred. Preparation of 5-FVA from adipic acid ("Reaction 8") According to the present invention, adipic acid can be used to prepare 5-FVA by reducing one of the carboxylic acid groups. This item can be achieved chemically, for example by selective chemical reduction, which may be achieved by a protonation reaction of a carboxylic acid group or by biocatalysis. In a preferred method of the invention, the preparation comprises a biocatalytic reaction in the presence of a biocatalyst capable of reducing the catalytic carboxylic acid. The biocatalyst can use NADH or NADPH as an electron donor. Therefore, an enzyme having such catalytic activity is referred to as an aldehyde dehydrogenase. Preferably, the aldehyde dehydrogenase is selective for the enzyme substrate adipic acid.

The enzyme which catalyzes the reduction of the carboxylic acid is selected in particular from the group consisting of oxidoreductases (EC 1.2.1), preferably selected from the group consisting of aldehyde dehydrogenase (EC 1.2.1.3, EC 1.2.1.4). And EC 1.2.1.5), malonate semi-dehydrogenase (EC 1.2.1.15), succinic acid semialdehyde dehydrogenase (Ec 1.2.1.16 and EC 1.2.1.24); glutamic acid semialdehyde dehydrogenase (EC 1.2.1.20), amine adipic acid semialdehyde dehydrogenase (EC 1.2.1.31), adipic acid semialdehyde dehydrogenase (EC i2163), also known as 6-ketohexanoate dehydrogenase group . The adipic acid semialdehyde dehydrogenase activity has been described in the KEGG database for the decomposition of caprolactam. In particular, 6-ketohexanoate dehydrogenase can be used. An example of a 6-ketohexanoate dehydrogenase is an enzyme comprising the sequence represented by the sequence ID 127, 128 or its homologue. 32 201033370 The body may be derived from or derived from any organism. Organic: Prokaryotic or eukaryotic. The particular organism may be selected from the group consisting of bacteria, archaea, yeast, fungi, r protozoa, plants and animals (including humans). In the case of a non-moving rod, the bacterium is selected from the group consisting of: (especially Acinetobacter species NCIMB9871), Rasta genus 'Bod's M,,, Chinese roots;: genus, Burkholder, bacterium, genus Flavobacterium, γ!, genus Nitrobacter, Nitrobacter, Brucella (especially Brucella melanogaster), Pseudomonas, Agrobacterium β/Agrobacterium tumefaciens, Bacillus, Listeria, alkaloids, Bacillus, Corynebacterium, and Flavobacterium. ^ : In one embodiment, the organic system is selected from the group consisting of yeast and fungi, in particular selected from the group consisting of the genus Trichophyton (especially black bacillus and venom) and the genus Penicillium (especially P. chrysogenum) The group formed. In one embodiment, the organism is a plant, particularly Arabidopsis, more particularly Arabidopsis. Preparation of φ 6-ACA ("Reaction 6") In one embodiment of the invention, 5-FVA was used to prepare 6-ACA. In one embodiment, 6-ACA is obtained by hydrogenating P-02 with 6-mercaptohexanoic acid obtained by reacting 5-FVA with hydroxylamine (FO Ayorinde, EY Nana, PD Nicely, AS Woods, EO Price, Cp Nwaonicha J. Am. Oil Chem. Soc.l997, 74, 531-538 also references the synthesis of the homolog 12-amine dodecanoic acid). 6-ACA can be prepared in high yield via a reductive amination reaction using a hydrogenation catalyst such as Ni in a Si〇2/Al203 support using ammonia 5-FVA, as in 33 201033370 EP-A 628 535 or DE 4 322 065 Said for 9-amine decanoic acid (9-amine geranyl acid) and 12-amine undecanoic acid (12-amine lauric acid). In yet another embodiment, 6_ACA is prepared in a biocatalytic manner. In a preferred method, the preparation of 6-ACA from 5-FVA comprises enzymatic reaction in the presence of an enzyme selected from the group consisting of a transaminase (E c 2.6.1) which catalyzes a transamination reaction in the presence of an amine donor. . % ® god makeup has a 6-form 5-FVA converted to 6_ACa

The transaminase is particularly selected from the group consisting of the following transaminase: the mammalian genus is a perennial hawthorn, more particularly a perennial genus of the fern, especially the unilateral fern or the north Iron-toothed fern; Carob nia) is more particularly long-horned beans (cerat〇nia 8 丨 仙 ;); Red bacillus is especially RhRdd〇 bacter sphaer〇ides; Staphylococcus is particularly Staphylococcus aureus ( Staphykeoeeus aureus); Vibrio genus is river = Vibrio; Pseudomonas is especially Pseudomonas aeruginosa; Rhodopseudomonas is cytosine genus (four) Bacillus vegetative and hay (four) · silicosis Acid genus, Neisseria; or yeast, especially for Saccharomyces cerevisiae, is a nucleus of Wei riding enzyme, _ is particularly difficult to derive from η kidney, donor liver, mammal heart or mammalian brain. _ / amine Γ selected from (four) group of lower rides: from the mammalian kidney 4 different: · ·: two:: transaminase _ is derived from the pig kidney "transfer amine, special = free: it: from "Mammal liver" _ The heart of the winter turns the material (four) rides the heart of the aspartic acid to transfer 34 201033370 from the mammalian liver 4_amine-butyric acid transaminase; obtained from the mammalian cerebral palsy especially Since the 4_amine of pig liver from the human, pig, or rat brain, the 4_amine_butyric acid transamination m material is especially the ketoadipate-proline amide transamination peak, and the heart of the genus Amino acid transaminase; obtained from Escherichia coli (4) adipic acid family advisory + butyric acid transaminating, or from a: amine + enzyme adipic acid transaminase, especially from thermophilic Clostridium citrate

° (Pyrobaculum 1SlandlCUm) provides the appropriate 2-amine adipic acid transamination. In a particular embodiment, the transaminase used comprises according to sequence 82, sequence I々D 83, sequence ID 84, sequence m 134, sequence id 136, sequence,

Or the order of this kind of money - the same as the green - amino acid phase. Sequence 1 86 (wild and 88 (codon-optimized to show the coding sequence of the enzyme represented by the sequence m 82 (=87). Sequence ID 89 (wild type) and 91 (codon optimization)) represented by sequence 1D The coding sequence of the enzyme represented by 82 (=87). Sequence ID 133, sequence ID 135, and sequence id 137 represent the sequence of sequence ID 134, sequence ID 136, and sequence 138, respectively. The special amine donor may be selected from ammonia. a group consisting of ammonium ions, amines and amino acids. Suitable amines are first amines and second amines. Amino acids have D-configuration or L-configuration. Examples of amine donors are alanine, bran Amine acid, isopropylamine, 2-amine butane, 2-amine heptane, benzoguanamine, 1-phenyl-1-amine ethane, glutamine, tyrosine, albinoic acid, aspartic acid, point-amine Isobutyric acid; S-malonic acid, 4-amine butyric acid, and α-amine adipic acid. In still further preferred embodiments, the method for preparing 6-ACA is included in 35 201033370

a biocatalytic reaction in the presence of an ammonia source capable of catalyzing a reductive amination reaction, the enzyme being selected from the group consisting of an oxidoreductase (EC 1.4) acting on the ch_nh2 group of the donor, in particular selected from the group consisting of A group consisting of amino acid dehydrogenase (EC 1_4.1). In general, an appropriate amino acid dehydrogenase has 6-aminocaproic acid 6-dehydrogenase activity to catalyze the conversion of 5-FVA to 6-ACA; or has alpha-amine pimelic acid 2-dehydrogenase activity to catalyze AKP Convert to AAP. Particularly suitable amino acid dehydrogenases may be selected from the group consisting of diamine pimelate dehydrogenase (EC 1.4.1.16), lysine 6-dehydrogenase (EC 1.4.1.18), and facial acid dehydrogenase ( EC 1.4.1.3; EC 1.4.1.4) and a group consisting of leucine dehydrogenase (EC 1.4.1.9). In one embodiment, the amino acid dehydrogenase may be selected from the group consisting of amino acid dehydrogenases classified as follows: glutamate dehydrogenase (EC 1.4.1.3) that acts as a receptor with NAD or NADP, A face acid dehydrogenase (EC 1.4.1.4), leucine dehydrogenase (ec 1.4.1.9), diamine pimelate dehydrogenase (EC 1.4.1.16), which functions as a receptor with NADP, and Amino acid 6-dehydrogenase (EC 1.4.1.18). The amino acid dehydrogenase may in particular be derived from an organism selected from the group consisting of: Corynebacterium, in particular Corynebacterium glutamicum; Proteus, in particular Proteus vulgaris; Agrobacterium, in particular Agrobacterium tumefaciens; the genus of the genus Bacillus is particularly a fixed thermophilic bacterium; the genus Acinetobacter is particularly ADP1 of the Acinetobacter species; the genus Astragalus is particularly a Ralstonia S〇lanaceamm; the genus Salmonella is particularly a typhoid bacillus Saccharomyces, especially Saccharomyces cerevisiae; Brevibacterium genus, especially Brevibacterium flavum: and Bacillus sp. (4) are spherical spores, cactus buds g or grass: bacteria. For example, a suitable amino acid dehydrogenase may be selected from the group consisting of diamine pimelate dehydrogenase from Bacillus sphaeroides; diamine 201033370 pimelic acid dehydrogenase from Brevibacterium; a diamine pimelic acid dehydrogenase of the genus Bacillus, in particular a diamine pimelic acid dehydrogenase derived from Corynebacterium glutamicum; a diamine pimelic acid dehydrogenase obtained from a Proteus species, in particular a diamine pimelic acid dehydrogenase derived from Proteus vulgaris; an lysine 6-dehydrogenase derived from Agrobacterium tumefaciens; a lysine 6 derived from the genus Bacillus licheniformis - dehydrogenase; glutamate dehydrogenase (EC 14丨3) derived from Acinetobacter with NADH or NADpH as a cofactor, especially from the glutamate dehydrogenase of the Acinetobacter sp. ADP1; A glutamic acid dehydrogenase from the genus Rasta (EC 1.4.1.3), especially from the glutamate dehydrogenase of the genus Phytophthora; a glutamine derived from the genus Salmonella with NADPH as a cofactor Acid dehydrogenase (EC 1.4.1.4) 'Specially obtained from glutamate dehydrogenase of Salmonella typhi; obtained from the face acid of the genus Saccharomyces Enzyme (EC 4 Shang Shu 4), in particular from beer

Saccharomyces glutamate dehydrogenase; amylin dehydrogenase from the genus Brevibacterium (EC 1.4.1.4), especially from the sulphate dehydrogenase of Brevibacterium brevis; and white from Bacillus Ship dehydrogenation - not derived from cactus buds g or Bacillus subtilis leucine dehydrogenase. In one embodiment, the 6 ACA prepared in the process of the invention is used in the preparation of the product. Such a process comprises recycling 6-amine hexanoic acid in the presence of a biocatalyst as needed. The reaction conditions for any of the biocatalytic steps used in the context of the present invention can be selected based on the known conditions of the biocatalyst, particularly the conditions of the enzyme, the information disclosed herein, and optionally via several routine experiments. In principle, the pH of the reaction medium used may be selected from a wide range of limits as long as the biocatalyst is active under the pH conditions. According to 37 201033370 biocatalyst and other factors, alkaline, neutral or acidic conditions can be used. Where the method comprises the use of a microorganism, e.g., for expressing an enzyme that catalyzes the method of the invention, the pH is selected such that the microorganism can perform its intended function. The pH may in particular be selected from 4 pH units below neutral pH and 2 pH units above neutral pH, ie in the range of pH 3 to pH 9 at 25 ° C in the case of a substantially aqueous system. . If the water is the sole solvent or the main solvent (greater than 50 wt.% on a total liquid basis, especially greater than 90 wt.%), the system is considered to be aqueous, wherein for example a small amount can be dissolved (less than 5 〇 wt % based on the total liquid) The concentration of the alcohol or other solvent (e.g., as a carbon source), particularly less than 10 wt.%, allows the microorganisms present to remain active. Particularly in the case of using yeast and/or fungi, acidic conditions are preferred, particularly based on a substantially aqueous system at 25 ° C and a pH at pH 3 to pH 8. Can acid and/or alkali be adjusted if needed? 11 or buffer the pH with an appropriate combination of acid and assay. In principle, the culture conditions can be selected from a wide range as long as the biocatalyst exhibits sufficient activity and/or growth. Such conditions include aerobic, micro-oxygen, oxygen-limited and anaerobic conditions. An anaerobic condition is defined herein as a condition free of any oxygen, or in which substantially no oxygen is consumed by the biocatalyst, particularly by microorganisms. 'Usually less than 5 millimoles per liter. Oxygen consumption in hours, especially equivalent to less than 2.5 millimoles per liter per hour or less than i millimoles per liter of oxygen consumption. There are milk conditions in which a sufficient amount of oxygen for unrestricted growth dissolves in the medium and thus supports at least a war/L of hour, more preferably more than 2 〇m, liters, hours, and more preferably more than 5 gram. Mohr / liter hour and best greater than 38 201033370 1 Saki Mo / L · hour oxygen consumption rate. The finite oxygen condition is defined as the condition in which the oxygen of the gas is limited. Oxygen is determined by the party's transfer from gas to liquid upper limit, that is, usually at least = the lower limit of the part is 2.5 mM per liter of anaerobic conditions or at least 5 =: / liter·hour and especially at least The upper limit is determined by the lower limit of the aerobic condition. Oxygen limited condition

The actual amount of L used in the equipment used is determined by the composition, composition, and microbial density. The nature of transfer, the microorganisms used make Hi degree ===_ otherwise _ shows substantial activity, then at least blowing, more temperature is at least 〇 °c, special catalysis #丨, ^ 夕 is 2 〇 < :. The highest temperature expected is based on the raw material. Usually, the highest temperature is known to the art, for example, in the case of two doses, as indicated by the product information sheet, or may be based on the stipulations of the Department of Health (4). Wen Lai often refers to thieves 22 or below, especially below SOW, and more particularly, solvents, additional reagents and other auxiliaries such as cofactors (such as Μ·· and/or nad/nadh) can be selected based on known reaction principles. Auxiliary factors) to achieve or add to a specific reaction 'and / or take measures to move the balance to the desired side of the 4th temple to the outside of the host organism for biocatalytic reactions, used in 39 201033370 to maintain enough in this medium In the case of active saki, a reaction medium containing an organic solvent at a still concentration (for example, more than 50 wt.% or more than 9 〇wt.%) may be used. The succinic acid (ester or thioester) and acetic acid (ester or thioester) used in the process of the invention can in principle be obtained in any manner. Succinic acid, for example, is naturally formed as an intermediate of the citric acid cycle (Krebs cycle) or as an end product of cellular metabolism. This can be obtained from a renewable carbon source using a suitable biocatalyst. Biocatalysts In particular, microorganisms can be used to produce succinic acid from a suitable carbon source. The microorganism can be a prokaryote or a eukaryote. The microorganism can be recombinant or wild type. In recombinant microorganisms, metabolism can be altered to increase succinic acid production and productivity above a suitable carbon source. Methods for increasing succinic acid production have been described for prokaryotic organisms in Song and Lee, Enzymes and Microbial Technology, 2006, 39: 352-361. Succinic acid can also be produced by eukaryotes. Additionally and additionally, an adaptation adaptation may be applied, such as illustrated in U.S. Patent No. 2007/111294. The succinate or thioester can be obtained from succinic acid in either manner. A particular succinate or thioester can be obtained from succinic acid using a biocatalyst. Particularly via the use of a hydrazine comprising a group selected from the group consisting of acid thiol ligases (EC 6.2.1), preferably selected from the group consisting of succinyl-CoA synthetase (EC 6.2.1.4 and EC 6.2.1.5) A catalyst for the enzyme consisting of the group, which can obtain butadiene-CoA from succinic acid. Additionally or alternatively, butadiene-CoA can be obtained from succinic acid via the use of a biocatalyst comprising an enzyme selected from the group consisting of CoA transferase (EC 2.8.3), as specified for Reaction 7. The succinate or thioester can also be obtained in any way from the 201033370 molecule other than succinic acid. Specifically, using a biocatalyst comprising a 2-ketoglutarate dehydrogenase complex, butadiene 醯c〇A can be obtained from 2-mertoglutaric acid. It is known to those skilled in the art that the ketoglutarate dehydrogenase complex is a multi-enzyme complex involved in the TCA cycle. Additionally or alternatively, using a biocatalyst comprising 2-mercapto-succinic acid: ferredoxin oxidoreductase (EC 12.73), butadiene-CoA can be obtained from 2-ketoglutaric acid. Acetic acid is the natural intermediate or end product of cellular metabolism. Thus acetic acid can be obtained from a renewable carbon source via the use of a suitable biocatalyst. Biocatalysts In particular, microorganisms can be used to produce succinic acid from a suitable carbon source. The microorganism can be a prokaryote or a eukaryote. The microorganism can be recombinant or wild type. Acetic acid vinegar or thioacetate can be obtained from acetic acid in either manner. In particular, by using an enzyme comprising an enzyme selected from the group consisting of acid thiol ligase (EC 6.2.1), preferably acetamidine-CoA synthetase (EC 6.2.1.1 and EC 6.2.1.13) Catalyst, acetic acid _C〇A can be obtained from acetic acid. Additionally or alternatively, acetamidine-c〇A can be obtained from acetic acid using a biocatalyst comprising an enzyme selected from the group consisting of c〇A transferase (EC 2.8.3) as defined in Reaction 7. The acetate or thioacetate may also be obtained from a molecule other than acetic acid in any manner. Specifically, the use comprises a compound selected from the group consisting of pyruvate dehydrogenase, pyruvate dehydrogenase (NADP+) (EC 1.2.1.51), and propionate citrate lysing enzyme (EC 2·3·1·54). Or a biocatalyst capable of efficiently converting a propionic acid into a biocatalyst or a group of enzymes of acetamidine _c〇A, and obtaining acetamidine-CoA from pyruvic acid, as known to those skilled in the art, pyruvate The hydrogenase complex is a multi-enzyme complex that converts pyruvate to ethylene-CoA. Using a biocatalyst comprising an enzyme selected from the group 41 201033370 consisting of an oxidoreductase (EC 1.2.1), acetamyl-CoA can also be obtained from acetaldehyde, preferably the enzyme is selected from dehydrogenation from an aldehyde. Enzyme (acetylation) (EC 1.2.1.10), fatty acid acetamidine-CoA reductase (EC 1.2.1.42), butyraldehyde dehydrogenase (Ec 1.2.1.57) and succinic acid half-acid dehydrogenase (acetamidine) a group consisting of (described in Sohling et al. 1996. JBacteriol. 178: 871-880). When the biocatalyst is a eukaryote, the supply of Ethyl-CoA can be increased by over-characterizing a homologous gene and/or a non-homologous gene encoding an enzyme that converts a catalytic precursor molecule into an Ethyl-CoA molecule, preferably increased in Supply of acetaminophen-CoA in the cytosolic compartment of the host cell. The precursor molecule is, for example, acetic acid as described by Shiba et al., Metabolic Engineering, 2007, 9: 160-8. In the excellent process of the present invention, particularly for the preparation of 6-ACA adipic acid or an intermediate compound for 6-ACA or adipic acid, for use in 6-ACA, adipic acid or an intermediate thereof The whole cell biotransformation of the enzyme matrix comprises the use of a microorganism in which one or more enzymes which catalyze any of the foregoing reactions are produced and a carbon source for the microorganism. The carbon source particularly contains at least one compound selected from the group consisting of a monohydric alcohol, a polyhydric alcohol, a carboxylic acid carbon dioxide, a fatty acid, a glyceride, and a mixture comprising any of the compounds. Suitable monohydric alcohols include sterols and ethanol. Suitable polyols include glycerin and carbohydrates. Suitable fatty acids or glycerides are especially provided in the form of edible oils, preferably vegetable-derived edible oils. Water-blocking compounds are particularly used because carbohydrates are generally available in large quantities from biorenewable sources such as agricultural products such as agricultural waste. Preferably, the carbohydrate used is selected from the group consisting of glucose, fructose, sucrose, milk 201033370 sugar, sucrose, starch, cellulose and hemicellulose. Particularly preferred are glucose, sugar-containing sugar, and glucose-containing polysaccharides. In a particular method of the invention, the method is a fermentation process. Such methods, in particular, comprise a cell comprising a biocatalyst, optionally contacting a host cell as described herein with a fermentable carbon source, wherein the carbon source comprises any compound that will be converted to a pre-prepared compound, or wherein the cells are prepared The compound to be converted into a compound to be prepared from the carbon source. The cells comprising one or more of the cells used to catalyze the reaction step in the method of the invention can be constructed using molecular biotechnology known in the art. For example, if one or more biocatalysts are to be produced in a non-homologous system, such techniques can be used to provide a vector comprising one or more genes encoding one or more of the catalysts. A vector comprising one or more of the genes can comprise one or more sputum elements operably linked to one of the genes encoding the biocatalyst, such as one or more promoter genes. The term "working link" as used herein refers to a functional relationship of a polynucleotide element (or a coding sequence or a nucleic acid sequence). A nucleic acid sequence is a "workable link" when it is functionally related to other nucleic acid sequences. For example, if the right promoter gene or the booster gene affects the transcription of the coding sequence, then the promoter gene or the booster gene line is functionally linked to the coding sequence. As used herein, the term "initiating gene" refers to a nucleic acid fragment that is used to control the transcription of one or more genes upstream of the transcriptional orientation relative to the transcriptional origin of the gene, structurally rDNa-dependent. The location of the RNA polymerase, the location of the transcriptional initiation, and the presence of any other genomic sequence including, but not limited to, the transcription factor 纟士入43 201033370 position, repressor protein and activated protein binding sites, and accustomed The skilled person is known to act directly or indirectly to mediate transcription by the promoter gene: any other nuclear sequence. The "constitutive" promoter gene has a heterogeneous promoter base under most environmental and developmental conditions. The "turning material" is an active promoter that is active under environmental or developmental regulation. "Homolog" - a word used to indicate the relationship between a given (recombinant) nucleic acid or a multi-peptide molecule and a given host organism or host cell, must be understood to be expressed from the suspicion or the multi-peptide Molecules are made from host cells or organisms of the same species, preferably of the same variety or of the same germline. The promoter which can be used to express the transcript nucleic acid sequence (such as the one described above) for use in the method of the present invention may be a difficult acid, a sequence, or may be the nucleic acid sequence (coding sequence). Non-homologous, system, * by working bond. Preferably, the promoter gene is homologous, i.e., the promoter gene is endogenous to the host cell. If a non-homologous promoter gene (relative to the nucleic acid sequence encoding the enzyme of interest) is used, '贞m non-μ promoter base _ can be made to have a higher steady-state concentration than the promoter gene of the clone A coding sequence transcript is included (or a greater number of transcript molecules, i.e., mRNA molecules, can be produced per unit time). Suitable promoter genes in this context include constitutive and inducible natural promoter genes as well as genetically engineered promoter genes, which are well known to those skilled in the art. A "potent constitutive promoter gene" is a promoter gene that causes mRNA to start at a higher frequency than a natural host cell. Examples of such potent constitutive promoter genes in Gram-positive microorganisms include sp〇126, 44 201033370 SP01-15, veg, pyc (pyruvate tickase promoter gene) and &1115^. Examples of the inducible promoter gene in Gram-positive microorganisms include the IPTG-inducible Pspac promoter gene, the xylose-inducible PxylA promoter gene. Examples of constitutive promoter genes and inducible promoter genes for Gram-negative microorganisms include tac, tet, trp-tet, lpp, lac, lpp-lac, laclq, T7, T5, T3, gal, trc, ara (PBAD) ), SP6, λ -PR, and λ -Pl. When used in a nucleic acid (DNA or RNA) or protein, the term "non-homologous" refers to a nucleic acid or protein that does not naturally occur as part of the organism, cell, genome or DNA sequence or RNA sequence in which it is present. , or the nucleic acid or protein is present at a different location or different DNA or RNA sequence from a different cell or genome than the naturally occurring one. Non-homologous nucleic acids or non-homologous proteins are not endogenous to the cell into which they are introduced, but instead are derived from other cells or are produced synthetically or recombinantly. Roughly, but not necessarily, such nucleic acids encode proteins in which the cells in which the DNA is transcribed or expressed are not normally produced. Similarly, exogenous RNA encodes a protein in which the exogenous RNA normally does not express. Non-homologous nucleic acids and proteins can also be referred to as foreign nucleic acids or proteins. Those skilled in the art will recognize that non-homologous nucleic acids or foreign nucleic acids or proteins of cells expressing the nucleic acid are encompassed herein by the term non-homologous nucleic acid or protein. The method according to the invention can be carried out using an organism which can be a host organism, in particular a host microorganism or a wild-type microorganism. Thus, the invention also relates to a novel (host) cell which may be a microorganism comprising a biocatalyst which catalyzes at least one reaction step of the method of the invention, preferably the cell is capable of producing an enzyme or a plurality of hydrazines thereby catalyzing 45 201033370 in the process of the invention two or more reaction steps. The invention also relates to a novel vector comprising one or more genes encoding one or more enzymes that catalyze at least one of the reaction steps of the methods of the invention. In one embodiment, a cell or a vector comprising a nucleic acid sequence can be provided. The nucleic acid sequence can be a recombinant nucleic acid sequence encoding a 5-redog-2-pentenyl ester or thioester hydrogenase activity, particularly 5- a plum of carboxy-2-pentene hydrazine hydrogenase activity. Preferably, the cell further comprises a nucleic acid sequence encoding at least one of the enzymes (the recombinant vector comprises one) selected from the group consisting of catalyzable hexamethylene ester or thioester, especially hexamethylene-C〇A, converted to 5 - FVA enzyme and a group of enzymes which can catalyze the conversion of diterpene or thioesters, in particular, the conversion of hexamethylene _C〇A to adipic acid. In particular, in an embodiment, wherein the cell comprises an enzyme that catalyzes the conversion of hexamethylene phthalate or thioacetate to 5-FVA, the cell preferably comprises (a recombinant vector, package 3 thereof) encoding to convert 5-FVA to 6 A nucleic acid sequence of one of the enzymes of ACA. Such hydrazines may in particular be enzymes having 5-FVA transaminase activity. In addition or in addition, the '(host) cell or vector comprises at least one of the following nucleic acid sequences: - the encoding can catalyze the reaction of butyl or thioester with acetate or sulfur sulphur to form a 3-ketone a nucleic acid sequence of one of the ester or thioester enzymes; - a nucleic acid sequence encoding one of the enzymes capable of catalyzing the formation of 3 - hydroxyhexanol or a thioester from 3 - keto hexanedicarboxylate or a thioester; 3-hydroxy hydroxy decyl ester or thioester to form a nucleic acid sequence of 5 - carboxy 46 201033370 -2-pentan vinegar or a carbon ester enzyme; - encoding catalyzed by 5-carboxy-2-pentene oxime ester or sulphur The ester forms a nucleic acid sequence of one of the enzymes of hexamethylene or thioester. One or more suitable genes are selected, in particular, from a gene encoding an enzyme as described above, more particularly from the coding according to sequence ID 1-67, 94, 96, 98, 1〇〇, 102, 103, 1〇5. The gene of the enzyme of any of 107, 109, lu, 113, ιΐ5, ιι6 or a homolog thereof. • The host cell can be a prokaryotic or eukaryotic cell. The particular host cell can be selected from the group consisting of bacteria, archaea, yeast, fungi, protists, plants and animals (including humans). - In other words, the host cell according to the present invention may be selected from the group consisting of the genus Vibrio spp., the genus Corynebacterium, the genus Escherichia, the genus Saccharomyces, the genus Pseudomonas, the genus Penicillium, The group consisting of the genus Pichia is particularly a host strain, such that the host cell can be selected from the group consisting of a large intestine rod, Bacillus subtilis, Bacillus licheniformis, Corynebacterium glutamicum, Black scorpion _ A group consisting of Pichia pastoris and Saccharomyces cerevisiae. It is preferred to produce a short-chain fatty acid such as succinic acid and/or acetic acid and/or its ester 3, which is 1 Sg of primary cells. Organisms that achieve this function are usually present in the tumor site of the opposite animal. In particular, it is preferred to co-produce an organism of succinic acid and acetic acid or an ester thereof or a thioester thereof. The microorganism can be a recombinant or wild type microorganism. In particular, microorganisms which can produce dipterin include Escherichia coli, Acinetobacter (especially for succinogenes), Mannheimia (special:, ', butyric acid) M. succiniciproducens), Saccharomyces cerevisiae, 47 201033370 Phytophthora (especially black bacillus), Penicillium (especially P. simplicissimum) and Kaemwich Jantama, MJ Haupt, Spyros A. Svoronos, Xueli Zhang, JC Moore, KT Shanmugam LO Ingram. Other organisms described in Biotechnology and Bioengineering (2007) 99, 5: 1140-1153. Particularly suitable microorganisms for the production of acetic acid include Enterobacteriaceae (especially Escherichia coli, Salmonella and Shigella), Acetic acid bacteria (including Acetobcter (especially Acetobacter aceti), and Lactobacillus (especially for Gluconobacter oxidans), Acidomonas, Gluconacetobacter, Asaia, Kozakia, and Shaming Swaminathania, Saccharibacter, Neoasaia, Granulibacter, Clostridium (especially C. aceticum, Clostridium thermoaceticum) Thermoaceticum), C_ thermoautotrophicum, C. fonnicoaceticum, Clostridium clostridium, C. propkmicum, and the genus Coccidia (especially Escherichia) Macrococcal), Acetobacterium X (Acetobacterium X, especially Aw〇〇dii and A' wieringae), Lactobacillus (especially flat Lactobacillus) L plantamm), Lactobacillus brevis (L. breVUm), Bifidobacterium (especially B bifidum) and Leuconostoc. The present invention further relates to novelty Multi-peptides and nucleotide sequences encoding such multi-peptides. In particular, the invention further relates to amines comprising any of the constituents according to the sequence 201033370, ID 57' 68 72' 79' 85 and its homologs The invention relates to a species comprising an amino acid sequence according to any one of the sequence (1) 57, 68-72, 79, 85 and its homologues. The peptide encodes a multi-nucleotide acid. Next, the present invention will be exemplified by the following examples. Example Example 1: Summary method

Molecular and genetic techniques to show quasi-genetic and molecular biology techniques are generally known to the art world and have been described previously (Maniatis et al. 1982 "Molecular Transduction: A Laboratory Manual". Cold Harbor Laboratory 'Cold Spring Harbor, New York; Miller 1972" Molecular Genetics Experiment, Cold Spring Harbor Laboratory, Cold Spring Harbor; Sambrook and Russell 2001 "Molecular Transduction: Laboratory Manual" (Third Edition), Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, edited by F_ Ausubel et al. "Popular Programs in Molecular Biology", Green Publishing and Willy Technology, Inc., New York, 1987). The plastids and germ lines pBAD/Mc-His C and pET21d were obtained from Invitrogen (Casper Bed, California, USA) and EMD Biosciences (Danstedt, Germany). pF113 (derivative of pJF119EH (Ftirste, JP, W. Pansegrau, R. Frank, H. Blocker, P. Scholz, M. Bagdasarian and E. Lanka. 1986 in the multi-host range tacP expression vector in the RP4 primer region of the colony Molecular selection 'gene 48: 131) which contains two notl positions at positions 515 and 5176 'starting with the tac promoter gene as a starting point number), pACYC-tac (Kramer, M. (2000). Untersuchungen 49 201033370 zum Einfluss Erhohter Bereitstellung von Erythrose-4-Phosphat und Phosphosenolpyurvat auf den Kohlenstofffluss in den Aromatenbiosyntheseweg von Escherichia coli. Berichte des Forschungszentrums Jtilich, 3824. ISSN 0944-2952 (Ph.D. thesis, Russdorf University) and pMS470 (Balzer, D.; Ziegelin, G·; Pansegrau, W.; Kruft, V_; Lanka, Ε·Nucleic Acid Research 1992, 20(8), 1851-1858) has been described as before. E. coli TOPIO (English micro gene company, Kasperd, California, USA) Used for all selection procedures. The large intestine rod top line Topi0 (夬微基因公司, Kasperd, California, USA),

Rv308 (ATCC31608) 'Rv308AaraB and BL21 A1 (British Microgenes, Kasperd, CA, USA) for protein expression. All carriers are allowed to have the same selection strategy by inserting a common link adjustment. The adjustment plan and the general selection plan are shown in Figure 2. Medium 2xTY medium (16 g/L tryptic, 10 g/L yeast extract, 5 g/L NaCl) was used for the growth of E. coli. Antibiotics (1 μg/ml ampicillin) were added to maintain the plastids. For induction of gene expression, using arabinose (for pBAD derivatives), iptg (for pMS470 and pF113 derivatives), and a combination of arabinose and IpTG (for pET21d derivatives in E. coli BL21-A1) The final concentrations were 0.005-0.2% (arabinose) and 0]_〇.5 mM (ipTG), respectively. Identification of plastids The quality of the system carrying different genes is recognized by genetic, biochemical and/or silky means commonly known in the art world, such as the anti-antibiotic anti- 50 201033370, PCR analysis or qualitative analysis of the transformed strain. Purification of bulk DNA, restriction analysis of purified plastid DNA or DNA sequence analysis. HPLC-MS Analytical Method for the Determination of Co A Derivatives The concentrations of hexamethylene-CoA and 6-carboxy-2,3-enhexanyl-CoA were determined by LC-Ms. Separation was carried out using an Agilent SB_C18 2. Γ 50 mm column using acetonitrile/water (pH = 75) buffered with 750 mg/liter of octyl ammonium acetate as the mobile phase. The flow rate is 3 〇〇 microliters / minute, with a gradient (start: 70% water, 3 minutes φ clock down to 58%, phase change to 45%, further reduced to 2〇% after ι·5 minutes, then The column was again equilibrated, in which the operating time was 7 minutes) ^ Elution was performed. In the electrospray free mode, LTQ orbitap was used to scan from m/z 765-900.己二醯_coA and 6_Rebel_2,3_稀己醯_c〇A were eluted at 2.25 minutes and 2.5 minutes, respectively. By observing the exact protonated molecule of the desired compound (Second-CoA; 894, 15123-894, 16017, 6-carboxy-2,3-diazepine-CoA: 892. 13437-892. 14329), the method The selectivity is enhanced. To determine the concentration, a standard curve of the compound prepared synthetically was used to calculate the response factor of the individual ions. Used to calculate the concentration in an unknown sample. Detection and quantification of adipic acid is described in Kippenberge, M.; Winterhalter, R.; Moortgat, G. Κ Anal. Bioanal. Chem. 2008, 392(7-8), 1459-1470. Example 2: Determination of 6-rebel 2,3·thin hexanyl-CoA reductase activity The composition of the putative 6-carboxy-2,3-enhexanyl-CoA reductase was selected from the database (Table 1). ). The target gene encoding the selected protein is optimized by codon pair 51 201033370 (using the method described in W008000632) and consisting synthetically (Geneart, Reynoldsberg, Germany). Target genes (e. g., secretion signals or peroxisome/mitochondrial target sequences) are removed from the amino acid sequence prior to optimization. This set of sequences can be identified by bioinformatics tools known to the artisan' as described in Emanuelsson et al. Nature 2: 953-971. In the optimization procedure, 'avoiding the internal shear position, the common cut-off position is introduced at the start and end points to allow for colonization according to the strategy shown in Figure 2. Such modifications may result in small changes in individual protein sequences and are not expected to alter the properties of individual proteins in any way. There is an isotopic ribosome binding position and leader sequence in front of each 〇RF to drive translation of pF113, pMS470 and pET21d. In pBAD, the translation initiation signal is provided by the vector. The target genes 'Adi4', 'Adi5', 'Adi8' and 'Adi9, were selected with and without all four plastids read to the C-terminal His-tag provided by the linker sequence. Protein expression in E. coli The initial culture was grown overnight in 96-well plates in 200 microliters of medium per well. Transfer 40-160 microliters to a 24-deep well plate containing 4 ml of medium. For the pBAD composition of E. coli TOP10 or E. coli rv308 Δ araB, this medium directly contains 5% arabinose for induction. The plates were incubated at 25 C on an orbital shaker (inf〇rs, 55 rpm). Adding an inducing group after 4-6 hours (〇 5mM ipTG for E. coli

Rv308, E. coli BL21 or pF113 and pMS470 in E. coli TOP10; 55 mM IPTG and 0.2% arabinose were used for pET21d in E. coli BL21A1, and the well plates were cultured for further 4 to 48 hours to harvest cells by centrifugation. 52 201033370 Preparation of cell-free extracts and purification of His-tags Cells obtained by small-scale growth (refer to the previous paragraph) obtained by centrifugation and discarded supernatants. The pellets formed during centrifugation were frozen at 1-2 for at least 16 hours and then thawed on ice. 2 ml of freshly prepared lysis buffer (5 〇 111 river potassium phosphate ρΗ7·5, 0-1 mg/ml DNAse I ( Roche (R〇che), Almere, NL) '2 mg/ml lysozyme, 〇5 mM MgS〇4, lmM dithiothreitol and protease inhibitors (complete mini-free ^^ 八八, Roche, Almere, NL, used according to the manufacturer's instructions))) was added to each well and the cells were resuspended by vigorous vortex plate for 2-5 minutes. To achieve dissolution, the plates were incubated for 30 minutes at room temperature in order to remove cell debris. The plates were centrifuged at 4 艽 and 6 〇〇〇 for 2 。 minutes. The supernatant was transferred to a new well plate and maintained on ice until further use. For the purification of His-tagged protein, ms Matiche Multitrap HP filter plates (GE Helthcare bioscience AB) 'Switzerland's Uppsala' are used according to the manufacturer's instructions. Enzyme matrix synthase matrix (JR Stern, A. del Campillo, J) · Biol. Chem., 1956, 985. AK Das, MD Uhler, AK Hajr a, J· Biol. Chem” 2000, 24333. H. Oku, N. Futamori, K. Masuda, Y. Shimabukuro, T. Omine, H. Iwasaki, Biosc. Biotech.

Biochem., 2003, 2107. Elvidge et al., J. Chem. Soc" 1953, 1793. F. Liu, HY. Zha, ZJ. Yao, J. Org. Chem., 2003, 6679-6684) and desired organisms The chemical reaction product (w〇2〇〇4/1〇6347) was synthesized by Synom (Groningen, NL) according to the published procedure. Enzyme catalyzed olefin-CoA assay analysis 53 201033370 Preparation of reaction mixture 'containing 50 mM Clock buffer (pH 7.5), 0.7 mM NADH and NADPH, and about 20 μΜ enzyme substrate 6-carboxy 2,3-ene hexose-CoA. 190 μl of the reaction mixture was dispensed into each well of a 96-well plate. Individual lines with a blank vector were prepared in the same manner as 10 microliters of cell-free extract for the control reaction. To initiate the reaction, 10 microliters of cell-free extract or purified protein was added to each well. The reaction mixture was at room temperature (2 〇 _25 ° C) culture for 15 minutes to 24 hours, online monitoring of 340 nm UV absorption. At the end, the reaction was terminated by adding an equal amount of methanol and the sample was centrifuged. The supernatant was transferred to a new well plate 'and It was stored at -80 ° C until further analysis by HPLC-MS. It was found that hexamethylene-CoA is shown in Table 1. Table 1: Enzyme catalytic assay所C醯A (number is indicated by relative peak area) Biocatalyst SeqlD# Modified 1 hexamethylene Adi4 63 Remove N-terminal 134 amino acid 3266 Adi5 96 196031 Adi8 60 Remove N-terminal 22 amine Acid 581859 Adi9 100 Remove N-terminal 12-amino acid 117077 - (vehicle control) 0 1 If changed, the resulting multi-peptide does not start with N-terminal methionine, then the resulting multi-peptide is 2 The modification was carried out by adding methionine at the N-terminus. The results were obtained by culturing E. coli BL21 containing the adi gene for 20 hours in pMS470. Other expression vectors and host lines (such as pET21d in Escherichia coli BL21-A1) were used. , pBAD/Myc-His C also obtained positive results in E. coli Rv308 and pF113 E. coli BL21) and different culture time (for example, 2 hours). Example 3: Production of adipic acid adipic acid biosynthesis path by non-homologous microorganisms The composition design is synthesized by one of the enzyme catalytic activities shown in Figure 3. The enzymes encoding these activities are recognized in the database. The target genes encoding these enzymes are optimized by codon pair and synthesized. Composition (Centre, Inc., Regentsberg, Germany In the optimization procedure, remove the internal clipping position, the target sequence of the non-period 201033370 (such as the secretion signal or the peroxysomal dry sequence), and introduce the restriction position at the start point and the end point to allow 4 shows the performance of the carrier assembly path. Such modifications can result in minor changes in the protein sequence' but it is expected that the properties of the individual proteins will never be altered. Each 〇RF is preceded by an apical ribosome binding position and a leader sequence to drive translation. Used for reactions 1, 2 and 3 ' using adi21+22+23 or adi26+27+28

combination. For reaction 7, use a combination of adi29, adi30 or adi24+25. For reaction 4, use adil-20 with adi8, adi6, adil3+12. Table 2: SeqlD of different Adi proteins. Protein Seq ID # Adi21 5 Adi22 18 Adi23 33 Adi24 119 Adi25 120 Adi26 3 Adi27 29 Adi28 14 Adi29 116 Adi30 117 • Composition of adipic acid-producing Escherichia coli germline To form adipic acid-producing Escherichia coli germline, complete The plastid encoded by the diacid pathway is transformed into a suitable host species for expression of the selected gene. The pMS470 construct containing the complete pathway was transformed into E. coli BL21, TOP10 and Rv308. pBAD/Myc-His C was transformed into E. coli TOP10 and Rv308AaraB. The composition in pF113 or pACYC-tac is co-transformed with a compatible pF113, pACYC-tac or pMS470 composition to allow the final germline to contain the complete adipic acid pathway. These plastids were co-transformed into E. coli TOPIO, BL21 and Rv308. 55 201033370 Preparation of adipic acid In order to produce adipic acid, the initial culture was carried out in a 96-well plate containing 2 μl of microliter of medium at 3 (TC growth overnight. 50 μl was transferred to a plate containing 4 ml of culture 1 The 24-well plate was grown in the pit for 4-6 hours, then the inducer for inducing the adipic acid pathway was added. The hunger was cultured for 12 hours to 72 hours. The well plate was centrifuged and the sample was prepared for LC_MS analysis. The supernatant and the sterol were mixed with 1:1 to precipitate the protein and then directly analyzed. The metabolite derived from the cell was extracted by resuspending the pellet in 1 ml of ethanol. The cell suspension was transferred to a test tube with a screw cap in a boiling water bath. Heat for 3 minutes. After centrifugation, the supernatant was transferred to a new tube and evaporated in an accelerated evaporator (Speed_vac). The dried sample was resuspended in 100 μl of the mobile phase and analyzed. Example 4: Preparation of 5-FVA from hexamethylene-CoA The HPLC-MS method for the determination of 5-FVA by selective reaction monitoring (SRM)-MS, measurement of transition m/z 129-83 detection of 5-FVA. The peak area of the 5-FVA peak eluted in about 6 minutes was calculated, and the concentration of 5-FVA was calculated. Calibration was performed using an external standard procedure. All LC-MS experiments were performed on an Ajicool 1200 LC system containing a Level 4 pump, autosampler and column oven coupling Ajicool 6410 QQQ triple quaternary MS LC conditions: Column: 50x4.6 mm (Nucleosil C18, 5 micron (Machery & Nagel) pre-column coupled to 250x4.6 mm OD Puwei Prevail C18, 5 micron (Altech) 56 201033370

Column temperature: room temperature eluent: A: water containing 0.1% formic acid B: acetonitrile with 0.1% citric acid Gradient: time (minutes) % stripping agent B 0 10 6 50 6.1 10 11 10 Flow rate: 1.2 ml /min before entering the MS flow 1:3 split injection volume: 2 microliters MS conditions: ionization: negative ion electric spray source conditions: ion spray voltage: 5kV temperature · · 350 ° C segmentation voltage and collision energy Optimized Scanning Mode: Selecting the Reaction Mode: Transition m/z 129-83 The composition of the composition was determined by the putative deuterium-CoA reductase (SeqlD 74, 75, 77, 79, 80, 139-148). The expression constructs were designed and prepared in the same manner as described in Example 2 using pBAD/Myc-His C and pET21d. All steps of protein expression, extraction and purification were carried out as described in Example 2, and the reaction mixture was prepared by enzyme-catalyzed hexamidine-CoA reductase assay, comprising 50 mM potassium buffer (pH 7.5), each 57 201033370 0.7 mM NADH and NADPH and l〇pm_i〇mM enzyme·substrate twice with-CoA. 190 microliters of the reaction mixture was dispensed into each well of a 96-well plate. The hexamethylene-CoA system was prepared as described in Example 2. To initiate the reaction, 1 〇 microliter of cell-free extract or purified protein was added to each well. Ten microliters of cell-free extract prepared in the same manner from the germline carrying the blank vector was used for the control reaction. The reaction mixture was incubated at room temperature (20-25 °C) for 15 minutes to 24 hours with on-line monitoring of 340 nm UV absorption. At the end, the reaction was stopped by adding an equal amount of methanol. The supernatant was transferred to a fresh well plate and stored until 5-FVA was detected by HPLC-MS. Measurement of 5-FVA verified that the selected hydrazine had ruthenium-CoA reductase activity. Example 5 Preparation of 6-AC Α λ from 5-F VA HPLC-MS analysis for the determination of 6-ACA Calibration: Calibration was performed by an external calibration line of 6-ACA (m/z 132-m/z 114, chamber Temperature 7.5 minutes). All LC-MS experiments were carried out at Yajilong iioo, equipped with a fourth-stage pump, deaerator, autosampler, column oven and single quaternary MS (Yage Cold, Germany ). The LC-MS conditions are: @ Column: 50*4 Newcastle (Maggie and Naja) + 250x4.6 Purwell C18 (Act), all at room temperature (RT) Eluent: Α = 0 · l (v / v) formic acid in ultrapure water B = acetonitrile (pa 'Merck (Merck)) Flow rate: 1.0 ml / min, before the MS into the stream with a 1: 3 splitting gradient: at t = 0 minutes starts at 1〇〇% (ν/ν)Α, and is maintained for 15 minutes, and changed to 8〇% (v/v) B in 15 minutes (t=30 minutes). The gradient was maintained constant at 8〇% (v/v) B from 3 minutes 58 201033370 to 31 minutes.

Injection volume: 5 μl MS detection: ESI(+)-MS Electrospray ionization (E SI) was performed in positive scan mode using the following conditions: m/z 50-500, 50V segmenter, O.lm/ The step size is 350 ° C dry gas temperature, 10 liters of nitrogen / minute dry gas, 50 psig atomizer pressure and 2.5 kV capillary voltage. The design of the target gene's selection of the attB is added to the ribosome binding site and all genes downstream of the initiation codon and downstream of the initiation codon to assist in the use of Gatway technology (British Microgenes Inc., Casper, CA, USA) Germany). Gene synthesis and plastid composition The synthetic gene line was obtained from DNA2.0 and the codon was optimized for E. coli expression according to the standard procedure of DNA 2.0. Vibrio fluvialis JS17 [SEQ ID No. 86], which encodes Vibrio fluvialis JS176J-transaminase [SEQ ID No. 82] and B. faecalis KBAB4 transaminase (ZP_01186960) [SEQ ID No 83], respectively. The transaminase gene of B. sphaericus KBAB4 [SEQ ID No. 89] was codon-optimized, respectively, and the obtained sequences [SEQ ID No. 88] and [SEQ ID No. 91] were obtained by DNA synthesis. The cells to which these genes are supplied are hereinafter referred to as Escherichia coli TOP10/pBAD-Vfl_AT and Escherichia coli TOPl〇/pBAD-Bwe_AT, respectively. Colonization by PCR

Highly reliable PCR ultramixer according to manufacturer's specifications (PCR 59 201033370

Supermix High Fidelity (AMD) uses PCR to amplify a variety of genes encoding biocatalysts from genomic DNA. The PCR reaction was analyzed by agarose gel electrophoresis, and a PCR product of the correct size was eluted from the gel using a QIAquick PCR purification kit set (Qiagen, Hilden, Germany). As described in the manufacturer's protocol, the purified PCR product was cloned into pBAD/Myc- using Gateway technology (British Microgene) through the introduced extraction site and pDONR-zeo (English microgene) as an entry vector. Hs-DEST expression vector. The sequence of the gene cloned by PCR was confirmed by DNA sequencing. In this way, the expression vector, pBAD-Bsu_gil6077991_AT (containing the gene represented by sequence ID 133, the peptide represented by coding sequence ID 134), pBAD-Pae_gi9946143_AT (using primers identified by sequence IDs 130 and 131), pBAD-Pae_gi9951072_AT (including The gene represented by sequence ID 135, the coding sequence Π) 136 represents the peptide), pBAD-Pae_gi9951630_AT (containing the gene represented by sequence id 137, and the peptide represented by sequence ID 138). E. coli TOP10 (British Microgene), which is a chemically competent pBAD constitutive body, was obtained to obtain a corresponding germline. Enzyme-catalyzed reaction for conversion of 5-mevalonic acid to 6-ACA Unless otherwise specified, the preparation reaction mixture contains 10 mM 5-methylvaleric acid, 20 mM racemic α-mercaptodecylamine and 200 μM pyridoxal 5,-phosphoric acid in 50 mM potassium phosphate buffer, pH 7.0. 1 〇〇 microliter of the reaction mixture was dispensed into each well of the well plate. To initiate the reaction, 20 microliters of cell free extract was added to each well. The reaction mixture was applied to a shaker at 37. (: Shake for 24 hours. In addition, the chemical blank mixture (without cell-free extract) and the biological blank group (E. coli TOP10 containing pBAD/Myc-His C) were cultured under the same conditions. By 60 201033370 hplc-ms The samples were analyzed. The results are summarized in the following table. Table 3: Formation of 6-aca biocatalyst 6-ACA concentration in the presence of transaminase [mg/kg] Bacillus TOPl〇/pBAD-Vfl AT 43* " -- -- T〇Pl〇/pBAD-Pae pBAD-Pae gi 994614^ 930 E. coli TOPlO/pBAD-Pae AT 25* E. coli TOP10/pBAD-Bwe AT 24* E. coli TOPl〇/pBAD-Bsu gil6077991 AT 288 Escherichia coli TOPlO/pBAD-Pae gi9951072 AT 1087 E. coli TOP10/pBAD-gi9951630 AT 92 E. coli TOP10 (bio blank group) of pBAD/Myc-His C 0.6 None (chemical blank group) Undetectable method differs in the use of 10 micro Liter of cell-free extract instead of 20 μl of 'pyridoxal-5'-phosphate concentration of 50 μΜ instead of 200 μΜ and the volume of the reaction mixture in the well is 190 μl instead of 1 μL, shown in the presence of transaminase Formation of 6-ACA from 5-FVA, and E. coli can catalyze this formation [Simplified description of the figure 3 Figure 1 shows the preparation of adipate or thioester from succinate or thioester; Figure 2 shows a comprehensive analysis of the transfer strategy, showing only relevant features and limited shear position; The figure shows the adipic acid biosynthesis pathway; Figure 4 shows the translocation strategy for assembling the adipic acid pathway. [Main component symbol description] (none) 61 201033370

SEQUENCE LISTING <110> DSM IP ASSETS B.V. WU, Liang

Trefzer, Axel Christoph De Wildeman, Stefaan Marie Andre Berg, Marco Alexander <120> Adipate (ester or thioester) synthesis <130> P84199PC00 <150> EP 08152595.8 <151> 2008-11-03 <160><170> Patentln version 3.3

<210> 1 <211> 419 <212> PRT

<213> Penicillium chrysogenum <400> 1

Met Ser Asn Ala Gin Gin Arg Leu Ser Gin Val Ser Ser His Phe Gly 15 10 15

Pro Gly Gly Lys Lys Gly Ala Ala Ala lie Thr Glu Lys His Pro Asp 20 25 30

Asp lie Val Val Thr Cys Ala Leu Arg Thr Ala Leu Thr Lys Gly Gly 35 40 45

Lys Gly Gly Phe Lys Asp Thr Ala Gly Ala Asp Leu Leu Ala Gly Val 50 55 60

Phe Lys Ala Val Leu Asn Lys Ser Gly Val Asp Pro Ser Ser Val Gin 65 70 75 80

Asp lie Ala Val Gly Ser Val Leu Ala Pro Gly Gly Gly Ala Thr Glu 85 90 95

Phe Arg Ala Ala Ala Leu Val Ala Gly Phe Pro Glu Ser Thr Ala Val 100 105 110

Lys Ser Leu Asn Arg Gin Cys Ser Ser Gly Leu Gin Ala lie Ala Asp 115 120 125 lie Ala Asn Ala lie Gin Ser Gly Met lie Asp Val Gly lie Gly Ala 130 135 140

Gly Val Glu Ser Met Ser Ser Gin Tyr Gly Pro Gly Ala Val Thr Glu 145 150 155 160

Phe Ser Asp Leu Leu Glu Ser His Pro Glu Ser Ala Asn Cys Lys Val 165 170 175

Pro Met Gly Val Leu Ser Glu Asn Met Ala Lys Asp Arg Gly Val Thr 180 185 190

Arg Ala Ser Gin Asp Ser Phe Ala Ala Gin Ser Tyr Gin Lys Ala Val 1 195 200 205

Ala Ala Gin Lys Ala Gly Leu Phe Asn Glu Glu lie Ala Pro Leu Asp 210 215 220

Val Lys Trp Thr Asp Pro Lys Thr Gly Glu Glu Lys Thr lie Thr Val 225 230 235 240

Lys Ala Asp Asp Gly Val Arg Gin Gly lie Thr Ala Glu Ser Leu Gly 245 250 255

Lys lie Lys Pro Ala Phe Ala Lys Asp Gly Ser lie His Ala Gly Asn 260 265 270

Ala Ser Gin lie Ser Asp Gly Ala Ala Ala Val Leu Leu Met Lys Arg 275 280 285

Ser Thr Ala Glu Arg Leu Gly Gin Lys lie Leu Gly Lys Tyr Val Thr 290 295 300

Ala Ser Val Val Gly Val Lys Pro Leu Leu Met Gly Val Gly Pro Trp 305 310 315 320

Lys Ala lie Pro Val Ala Leu Glu Lys Ala Gly lie Thr Lys Asp Asp 325 330 335

Val Asp lie Tyr Glu lie Asn Glu Ala Phe Ala Ser Gin Cys Val Trp 340 345 350

Cys Val Asn Glu Leu Gly lie Pro Ala Glu Lys Val Asn Pro Lys Gly 355 360 365

Gly Ala lie Ala Phe Gly His Pro Leu Gly Cys Thr Gly Ala Arg Gin 370 375 380

Val Ser Thr Leu Phe Thr Glu Leu Lys Arg Thr Asn Lys Lys lie Gly 385 390 395 400

Val Thr Ser Met Cys Val Gly Thr Gly Met Gly Met Ala Ala Val Trp 405 410 415

Val Ser Glu <210> 2 <211> 418

<212> PRT <213> Penicillium chrysogenum <400> 2

Met Ser Ser Pro Gin Gin Arg Leu Asn Ser Val Ala Asn Gin Leu Ala 15 10 15

Pro Gly Ser Ala Arg Gin Lys lie Leu Ala Lys Asn Pro Asp Asp Val 20 25 30

Val lie Thr Tyr Leu Ala Arg Thr Pro Leu Thr Lys Ala Arg Lys Gly 2 35 40 45

Gly Leu Lys Asp Thr Thr Val Asp Asp Leu Leu lie Ser Leu Leu Thr 50 55 60

Thr Val Arg Glu Lys Ser Asn Leu Asp Pro Asn Leu Val Glu Asp Val 65 70 75 80

Cys Val Gly Asn Val Leu Cys Pro Gly Ser Ala Tyr Val Ala Arg Ser 85 90 95

Ala Val Leu Ala Ala Gly Tyr Pro Val Thr Ala Ala Ala Ser lie Ala 100 105 110

Asn Arg Phe Cys Ser Ser Gly Leu Leu Ala Val Gin Asn lie Ala Asn 115 120 125

Gin lie He Ala Gly Ser lie Asp Val Gly Val Ala Val Gly Ala Glu 130 135 140

Ser Met Ser Lys Asn Ala Asp Gly Gly Ala Pro Glu Met Ser Glu Arg 145 150 155 160 lie Thr Lys His Pro lie Ala Ser Gin Asn Ser Gin Pro Met Gly Gin 165 170 175

Thr Ser Glu Asn Val Ala Asn Gin Phe Asn lie Ser Arg Glu Gin His 180 185 190

Asp Arg Phe Ala Ala Asn Ser Phe Gin Lys Ala Glu Arg Ala Gin Lys 195 200 205

Ala Gly Trp Leu Glu Asp Glu lie Val Pro Val Arg Thr Gin lie Lys 210 215 220

Asp Pro Lys Thr Gly Glu Val Lys Asp lie Val Val Val Arg Asp Asp 225 230 235 240

Gly lie Arg Tyr Gly Thr Thr Pro Glu Ser Leu Gly Lys Val Arg Ala 245 250 255

Ala Phe Pro Gin Trp Ala Pro Ser Ala Thr Thr Gly Gly Asn Ala Ser 260 265 270

Gin lie Thr Asp Gly Ala Ala Ala Leu Val Leu Met Lys Arg Ser Arg 275 280 285

Ala Gin Gin Leu Gly Gin Pro lie Val Ala Lys Phe Cys Gly Ala Thr 290 295 300

Val Ser Gly Leu Glu Pro Arg lie Met Gly lie Gly Pro Ser Leu Ala 305 310 315 320 lie Pro Lys lie Leu Ser Lys 325

Phe Asn Leu Ser Lys Asp Asp lie Asp 330 335 lie Phe Glu lie Asn Glu Ala 340

Phe Ser Ser Met Gly Val Tyr Cys Val 345 350

Asn Lys Leu Gly Leu Asp Glu 355

Ser Lys Val Asn Pro Arg Gly Gly Ala 360 365 lie Ala Phe Gly His Pro Leu Gly Ala Thr Gly Ala Arg Gin Val Val 370 375 380

Thr Ala Leu Ser Glu Leu Arg Arg Gin Asp Lys Arg Val Ala Val Thr 385 390 395 400

Ser Met Cys Val Gly Thr Gly Met Gly Met Ala Gly lie Phe Val Ser 405 410 415

Glu His <210> 3 <211> 418 <212> PRT <213> Penicillium chrysogenum <400> 3

Met Ala Ala Asp Arg Leu Ser Ser Leu Leu Ser His Leu Lys Pro Gly 15 10 15

Ala Thr Asn Gly Leu Ala Ala lie Thr Gin Lys Asn Pro Asp Asp Val 20 25 30

Val lie Thr Leu Ala lie Arg Thr Pro Leu Thr Lys Ala Arg Lys Gly 35 40 45 Gly Phe Lys Asp Thr Glu Leu Asp Tyr Met lie Tyr Ala Leu Leu Lys 50 55 60 Glu Thr Leu Ala Lys Ser Gin lie Asp Pro Ala Leu Lie Glu Asp Val 65 70 75 80

Cys Leu Gly Asn Val Asn Glu Val Lys Ala Ala Tyr Met Val Arg Ala 85 90 95

Ala Ala Leu Ala Ala Gly lie Pro His Thr Ala Gly Ala Ser Ser Val 100 105 110

Asn Arg Phe Cys Ser Ser Gly Leu Lys Ala Val Gin Asp lie Ala Asn 115 120 125

Gin lie Gin Leu Gly Ala lie Asp Val Gly Val Ala Leu Gly Ala Glu 130 135 140

Leu Met Ser Ala Ser Gly Asp Arg Leu Asp Arg Pro Phe Asn Glu Glu 145 150 155 160

Val Leu Arg Asn Gin Glu Ala Ala Asp Cys 165 170

Met Gin Pro Met Gly Gin 175

Thr Ser Glu Asn Val Gly Lys Asp Phe Asp lie Pro Arg Glu Gin Gin 180 185 190

Asp Arg Tyr Ala Ala Glu Ser Phe Arg Arg Ala Glu Ala Ala Gin Asn 195 200 205

Asn Gly Trp Leu Asp Asp Glu lie Ala Pro lie Ala Val Lys Val Lys 210 215 220

Asp Pro Lys Thr Gly Glu Val Lys Glu Val Thr Leu Ser Arg Asp Asp 225 230 235 240

Gly lie Arg Pro Gly Thr Thr Phe Glu Ser Leu Ser Lys lie Arg Pro 245 250 255

Ala Phe Pro Gin Phe Gly Asp Lys Ser Thr Gly Gly Asn Ser Ser Gin 260 265 270

Val Thr Asp Gly Ala Ala Ser Val Leu Leu Met Arg Arg Ser Lys Ala 275 280 285 lie Glu Leu Asn Gin Pro lie Leu Ala Lys Phe Cys Gly Ala Thr Val 290 295 300

Ala Gly Val Pro Pro Arg Val Met Gly lie Gly Pro Thr Ala Ala lie 305 310 315 320

Pro Lys Leu Leu Ser Lys Phe Gin Leu Asp Lys Asn Asp lie Asp lie 325 330 335

Tyr Glu lie Asn Glu Ala Phe Ala Ser Met Ala Val Tyr Cys Val Lys 340 345 350

Asn Leu Gly Leu Asp His Ala Lys Val Asn Pro Arg Gly Gly Ala lie 355 360 365

Ala Leu Gly His Pro Leu Gly Ala Thr Gly Ala Arg Gin lie Ala Thr 370 375 380 lie Leu Ser Glu Ala Arg Arg Thr Lys Ser Lys lie Leu Val Thr Ser 385 390 395 400

Met Cys lie Gly Thr Gly Gin Gly Met Ala Gly Leu Phe Val Asn Glu 405 410 415

Gin Leu <210> 4 <211> 413 <212> PRT <213> Penicillium chrysogenum <400> 4

Met Ala Ser Pro lie Pro Arg Gly Leu Arg Gin Val Leu Gin Lys Ser 15 10 15 201033370

Ser Asn Asp lie Val lie 20 Leu Ser Ser Leu Arg Thr Pro Val Thr Arg 25 30 Ala Lys Lys Gly Gly Phe 35 Lys Asp Ala Tyr Pro Glu Glu Leu Leu Ala 40 45 Ser Val Leu Gin Ala Thr 50 Leu Lys Ala Asn Pro Asn Leu Asp Pro Ala 55 60 Gin lie Asp Asp Val Leu 65 70 lie Gly Ser Val Leu Gin Glu Leu Gly Gly 75 80 Ala Lys Ala Gly Arg Met 85 Gly Gin lie His Ala Gly Phe Pro His Ser 90 95 Val Pro Phe Asn Thr lie 100 Asn Arg Gin Cys Ser Ser Gly Leu Ala Ala 105 110 lie Thr Thr lie Ala Asn Zero 115 Gly lie Arg Ala Gly Ala lie Asn Val Gly 120 125 Val Gly Gly Gly Met Glu Ser Met Thr Arg Asn Tyr Gly Ser Arg Ala 130 135 140 lie Pro Thr Val Leu Trp 145 150 Pro Glu Leu Lys Glu Ser Tyr Ser Gin Asp 155 160 Ala Arg Asp Cys lie Met 165 Pro Met Gly lie Thr Ser Glu Asn Val Ala 170 175 Ser Arg Tyr Gly Val Ser 180 Arg Ala Asp Gin Asp Ala Phe Ala Val Glu 185 190 Ser His Ala Lys Ala Ser 195 Ala Ala Gin Lys Ala Gly Arg Phe Asp Ser 200 205 At Glu lie Val Ser Val Thr ^ 210 215 Thr Lys Thr Leu Asp Pro Glu Asn Pro Asp 220 Ala Pro Ala Arg Asp Val 225 230 Thr Val Ser Gin Asp Asp Gly lie Arg His 235 240 Gly Leu Ser lie Glu Lys 245 Val Gly Ala Leu Lys Pro Ala Phe Ser Pro 250 255

Thr Gly Ala Ser Thr Ala Gly Asn Ser Ser Gin Val Ser Asp Gly Ala 260 265 270 Ala Ala Thr Leu Leu Met 275 Arg Arg Ser Thr Ala Glu Glu Leu Gly Leu 280 285

Ser Gly Ser lie Lys Ala Arg Trp Val Ala Ser Ala Val Ala Gly Cys 290 295 300

Ala Pro Asp Glu Met Gly Val Gly Pro Ala Val Ala lie Pro Lys Leu 305 310 315 320 6 201033370

Leu Gin Thr Val Gly Val Glu Val Pro Glu Val Gly Val Trp Glu lie 325 330 335

Asn Glu Ala Phe Ala Ser Gin Ala Leu Tyr Ser Val Arg Lys Leu Gly 340 345 350 lie Asp Gin Ala Lys Val Asn Pro Asn Gly Gly Ala lie Ala lie Gly 355 360 365

His Pro Leu Gly Ala Thr Gly Ala Arg Gin Leu Ala Thr Leu Leu Pro 370 375 380

Glu Leu Glu Arg Ser Gly Gin Glu lie Gly Val lie Ser Met Cys lie 385 390 395 400

Gly Thr Gly Met Gly Met Ala Gly Met Phe Val Arg Glu 405 410

<210> 5 <211> 401

<212> PRT <213> Acinetobacter sp. <400> 5

Met Leu Asn Ala Tyr lie Tyr Asp Gly Leu Arg Thr Pro Phe Gly Arg 15 10 15

His Ala Gly Glu Leu Ala Ser lie Arg Pro Asp Asp Leu Ala Gly Leu 20 25 30

Val lie Gin Arg Leu lie Glu Lys Thr Gly Val Ala Gly Ala Asp lie 35 40 45

Glu Asp Val lie Phe Gly Asp Thr Asn Gin Ala Gly Glu Asp Ser Arg 50 55 60

Asn lie Ala Arg His Ala Ala Leu Leu Ala Gly Leu Pro Val Thr Val 65 70 75 80

Pro Gly Gin Thr Val Asn Arg Leu Cys Ala Ser Gly Leu Ala Ala lie 85 90 95 lie Asp Ser Ala Arg Ala lie Thr Cys Gly Glu Gly Asp Leu Tyr lie 100 105 110

Ala Gly Gly Val Glu Ser Met Ser Arg Ala Pro Phe Val Met Gly Lys 115 120 125

Ala Glu Ser Ala Tyr Ser Arg Asp Ala Lys lie Tyr Asp Thr Thr lie 130 135 140

Gly Thr Arg Phe Pro Asn Lys Lys lie Val Ala Gin Tyr Gly Gly His 145 150 155 160

Ser Met Pro Glu Thr Gly Asp Asn Val Ala Val Glu Tyr Gly lie Ser 165 170 175 7

Arg Glu Gin Ala Asp Leu Phe Ala 180 Ala Gin Ser Gin Ala Lys Tyr Gin 185 190 Lys Ala Leu Glu Glu Gly Phe Phe Ala Gly Glu lie Thr Ala Val Glu 195 200 205

Val Ser Gin Gly Lys Lys Leu Pro 210 215 Pro Lys Gin Val Thr Glu Asp Glu 220 His Pro Arg Pro Ser Ser Thr Leu 225 230 Glu Ala Leu Ser Lys Leu Lys Pro 235 240 Leu Phe Glu Gly Gly Val Val Thr 245 Ala Gly Asn Ala Ser Gly lie Asn 250 255 Asp Gly Ala Ala Ala Leu Leu lie 260 Gly Ser Glu Val Ala Gly Gin Lys 265 270 Tyr Gly Leu Thr Pro Met Ala Lys 275 280 lie Leu Ser Ala Ala Ala Ala Gly 285 Val Glu Pro Arg Lie Met Gly Ala 290 295 Gly Pro lie Glu Ala lie Lys Lys 300 Ala Val Ala Arg Ala Gly Leu Thr 305 310 Leu Asp Asp Leu Asp lie lie Glu 315 320 lie Asn Glu Ala Phe Ala Ser Gin 325 Val Leu Ser Cys Leu Lys Gly Leu 330 335 Gly lie Asp Phe Asn Asp Pro Arg 340 Val Asn Pro Asn Gly Gly Ala lie 345 350 Ala Val Gly His Pro Leu Gly Ala 355 360 Ser Gly Ala Arg Leu Ala Leu Thr 365 Val Ala Arg Glu Leu Gin Arg Arg 370 375 Asn Lys Lys Tyr Ala Val Val Ser 380 Leu Cys lie Gly Val Gly Gin Gly 385 390 Ser Leu Ala Met Val lie Glu Asn Val 395 400 <210> 6 <211> 393 <212> PRT <213&gt ; Cl Ostridium kluyveri <400> 6 Met Arg Glu Val Val He Val Ser 1 5 Ala Val Arg Thr Ala lie Gly Ser 10 15 Phe Gly Gly Thr Leu Lys Asp Val 20 Ser Ala Val Asp Leu Gly Ala lie 25 30 201033370

Val lie Lys Glu Ala Val Lys Arg Ala Gly lie Lys Pro Glu Gin Val 35 40 45

Asp Glu Val lie Phe Gly Asn Val lie Gin Ala Gly Val Gly Gin Ser 50 55 60

Leu Ala Arg Gin Ser Ala Val Tyr Ala Gly Leu Pro Val Glu Val Pro 65 70 75 80

Ala Phe Thr Val Asn Lys Leu Cys Gly Ser Gly Leu Arg Thr Val Ser 85 90 95

Leu Ala Ala Ser Leu lie Ser Asn Gly Asp Ala Asp Thr lie Val Val 100 105 110

Gly Gly Ser Glu Asn Met Ser Ala Ser Pro Tyr Leu lie Pro Lys Ala 115 120 125

Arg Phe Gly Tyr Arg Met Gly Glu Ala Lys lie Tyr Asp Ala Met Leu 130 135 140

His Asp Gly Leu lie Asp Ser Phe Asn Asn Tyr His Met Gly lie Thr 145 150 155 160

Ala Glu Asn lie Ala Glu Lys Trp Gly lie Thr Arg Glu Asp Gin Asp 165 170 175

Lys Phe Ala Leu Ala Ser Gin Gin Lys Ala Glu Ala Ala lie Lys Ala 180 185 190

Gly Lys Phe Lys Asp Glu lie Val Pro Val Thr Val Lys Met Lys Lys 195 200 205

Lys Glu Val Val Phe Asp Thr Asp Glu Asp Pro Arg Phe Gly Thr Thr 210 215 220

Lie Glu Thr Leu Ala Lys Leu Lys Pro Ala Phe Lys Arg Asp Gly Thr 225 230 235 240

Gly Thr Val Thr Ala Gly Asn Ser Ser Gly lie Asn Asp Ser Ser Ala 245 250 255

Ala Leu lie Leu Met Ser Ala Asp Lys Ala Lys Glu Leu Gly Val Lys 260 265 270

Pro Met Ala Lys Tyr Val Asp Phe Ala Ser Ala Gly Leu Asp Pro Ala 275 280 285 lie Met Gly Tyr Gly Pro Tyr Tyr Ala Thr Lys Lys Val Leu Ala Lys 290 295 300

Thr Asn Leu Thr lie Lys Asp Phe Asp Leu lie Glu Ala Asn Glu Ala 305 310 315 320

Phe Ala Ala Gin Ser lie Ala Val Ala Arg Asp Leu Glu Phe Asp Met 325 330 335 9

Ser Lys Val Asn Val Asn Gly Gly Ala lie Ala Leu Gly His Pro Val 340 345 350 Gly Cys Ser Gly Ala Arg lie Leu Val Thr Leu Leu His Glu Met Gin 355 360 365 Lys Arg 370 Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys lie Gly Gly Gly 375 380 Gin Gly 385 Thr Ala Val Val Val Glu Arg 390 <210> 7 <211> 391

<212> PRT <213> Clostridium kluyveri <400> 7

Met Lys 1 Asp Ala Val lie Val Ser Ala Val Arg Thr Ala lie Gly Ser 5 10 15 Phe Gly Gly Thr Leu Lys Asp lie Ser Ala Val Asp Leu Gly Ala lie 20 25 30 Val lie Lys Glu Ala Val Lys Arg Ala Gly lie Lys Pro Glu Gin Val 35 40 45 Asp Glu 50 Val lie Phe Gly Asn Val lie Gin Ala Gly Leu Gly Gin Ser 55 60 Pro Ala 65 Arg Gin Ala Ala Val Lys Ala Gly lie Pro Val Glu Val Pro 70 75 80 Ala Phe Thr Leu Asn Lys Val Cys Gly Ser Gly Leu Arg Ser Val Ser 85 90 95 Leu Ala Ala Gin Leu lie Lys lie Gly Asp Asp Asp lie Val Val Val 100 105 110 Gly Gly Thr Glu Asn Met Ser Ala Ala Pro Tyr Leu Leu Pro Lys Ala 115 120 125 Arg Trp 130 Gly His Arg Met Gly Glu Gly Lys Leu Val Asp Ala Met lie 135 140 Lys Asp 145 Gly Leu Trp Glu Ala Phe Asn Asn Tyr His Met Gly lie Thr 150 155 160

Ala Glu Asn lie Ala Glu Lys Trp Gly lie Thr Arg Asp Met Gin Asp

Glu Phe 165 170 175 Ala Leu Ala Ser Gin Gin Lys Ala Glu Ala Ala lie Lys Ala 180 185 190 Gly Lys Phe Lys Asp Glu lie Val Pro Val Thr Val Lys Gin Lys Lys 195 200 205 10

Lys Glu lie lie Phe Asp Thr Asp Glu Phe Pro Arg Phe Gly Thr Thr 210 215 220 lie Glu Ala Leu Ala Lys Leu Lys Pro Ser Phe Lys Lys Asp Gly Thr 225 230 235 240

Val Thr Ala Gly Asn Ala Ser Gly lie Asn Asp Ala Ala Ala Ala Leu 245 250 255

Val Val Met Ser Ala Asp Lys Ala Lys Glu Leu Gly lie Lys Pro Leu 260 265 270

Ala Lys lie Val Ser Tyr Gly Ser Lys Gly Leu Asp Pro Thr lie Met 275 280 285

Gly Tyr Gly Pro Phe Tyr Ala Thr Lys Leu Ala Leu Glu Lys Ala Asn 290 295 300

Leu Ser lie Ala Asp Leu Asp Leu lie Glu Ala Asn Glu Ala Phe Ala 305 310 315 320

Ser Gin Ser Leu Ala Val Ala Lys Asp Leu Glu Phe Asp Met Ser Lys 325 330 335

Val Asn Val Asn Gly Gly Ala lie Ala Leu Gly His Pro Val Gly Cys 340 345 350

Ser Gly Ala Arg He Leu Val Thr Leu Leu Tyr Glu Met Gin Arg Arq 355 360 365

Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys lie Gly Gly Gly Met Gly 370 375 380

Thr Ala Leu lie Val Glu Arg 385 390 <210> 8 <211> 391

<212> PRT <213> Clostridium kluyveri <400> 8

Met Arg Glu Val Val lie Val Ser Ala Val Arg Thr Ala lie Gly Ser 1 5 10 15

Phe Gly Gly Thr Leu Lys Asp Val Pro Ala Val Glu Leu Gly Ala Val 20 25 30

Val lie Lys Glu Ala Val Lys Arg Ala Asn Val Lys Pro Glu Gin lie 35 40 45

Asp Glu Val lie Phe Gly Asn Val lie Gin Ala Gly Leu Gly Gin Ser 50 55 60

Pro Ala Arg Gin Ala Ala Val Lys Ala Gly lie Pro Val Glu Val Pro 65 70 75 80 11 201033370

Ala Phe Thr Leu Asn Lys Val Cys Gly Ser Gly Leu Arg Ser Val Ser 85 90 95

Leu Ala Ala Gin Val lie Lys Ala Gly Asp Ala Asp lie Val Val Val 100 105 110

Gly Gly Met Glu Asn Met Ser Ala Ala Pro Tyr Val Leu Pro Lys Ala 115 120 125

Arg Trp Gly His Arg Met Gly Glu Gly Lys lie Val Asp Ala Met lie 130 135 140

Lys Asp Gly Leu Trp Glu Ala Phe Asn Asn Tyr His Met Gly lie Thr 145 150 155 160

Ala Glu Asn lie Ala Glu Lys Trp Gly Leu Thr Arg Glu Glu Gin Asp 165 170 175

Glu Phe Ser Ala Ala Ser Gin Gin Lys Ala Glu Ala Ala Gin Lys Ala 180 185 190

Gly Lys Phe Lys Asp Glu lie Val Pro Val Thr Val Lys lie Lys Lys 195 200 205

Lys Glu Val Val Phe Asp Thr Asp Glu Tyr lie Lys Pro Gly Thr Thr 210 215 220

Val Glu Thr Leu Ala Lys Leu Arg Pro Ala Phe Lys Lys Asp Gly Thr 225 230 235 240

Val Thr Ala Gly Asn Ala Ser Gly lie Asn Asp Ala Ala Ala Ala Leu 245 250 255

Val Val Met Ser Ala Asp Lys Ala Lys Glu Leu Gly lie Lys Pro Leu 260 265 270

Ala Lys lie Val Ser Tyr Gly Ser Ala Gly Leu Asp Pro Thr lie Met 275 280 285

Gly Tyr Gly Pro Phe His Ala Thr Lys Ala Ala Leu Glu Lys Ala Asn 290 295 300

Leu Ser Val Ala Asp Leu Asp Leu lie Glu Ala Asn Glu Ala Phe Ala 305 310 315 320

Ser Gin Ser Leu Ala Val Ala Lys Asp Leu Glu Phe Asp Met Ser Lys 325 330 335

Val Asn Val Asn Gly Gly Ala lie Ala Leu Gly His Pro Val Gly Ala 340 345 350

Ser Gly Ala Arg lie Leu Val Thr Leu Leu His Glu Met Gin Arg Arg 355 360 365

Asp Ala Lys Lys Gly Leu Ala Thr Leu Cys lie Gly Gly Gly Met Gly 12 370 375 380

Thr Ala Leu lie Val Glu Arg 385 390 <210> 9 <211> 408

<212> PRT <213> Corynebacterium glutamicum <400> 9

Met Asn Pro Gin Asp lie Val lie Cys Ser Pro Leu Arg Thr Pro Val 15 10 15

Gly Ala Tyr Gly Gly Ser Phe Thr Gly Val Pro Val Glu Glu Leu Ala 20 25 30

Thr Thr Val lie Asn Ala lie Val Glu Ala Thr Gly lie Thr Gly Asp 35 40 45

Asp Val Asp Asp Leu lie Leu Gly Gin Ala Ser Pro Asn Gly Ala Ala 50 55 60

Pro Ala Leu Gly Arg Val Val Ala Leu Asp Ser Lys Leu Gly Gin Asn 65 70 75 80

Val Pro Gly Met Gin Leu Asp Arg Arg Cys Gly Ser Gly Leu Gin Ala 85 90 95 lie Val Thr Ala Ala Ala His Val Ala Ser Gly Ala Ala Asp Leu lie 100 105 110 lie Ala Gly Gly Ala Glu Ser Met Ser Arg Val Glu Tyr Thr Val Ser 115 120 125

Gly Asp lie Arg Trp Gly Val Lys Gly Gly Asp Met Gin Leu Arg Asp 130 135 140

Arg Leu Ala Glu Ala Arg Glu Thr Ala Gly Gly Arg Asn His Pro lie 145 150 155 160

Pro Gly Gly Met lie Glu Thr Ala Glu Asn Leu Arg Arg Glu Tyr Gly 165 170 175

He Ser Arg Glu Glu Gin Asp Lys lie Ser Ala Ala Ser Gin Gin Arg 180 185 190

Trp Gly Lys Ala Ala Asp Ala Gly Leu Phe Asp Asp Glu He Val Pro 195 200 205

Val Thr Val Pro Ala Lys Lys Arg Gly Gin Glu Pro Thr lie Val Ser 210 215 220

Arg Asp Glu His Gly Arg Pro Gly Thr Thr Val Glu Lys Leu Ala Ala 225 230 235 240

Leu Arg Pro lie Met Gly Arg Gin Asp Ala Glu Ala Thr Val Thr Ala 13 245 250 255

Gly Asn Ala Ser Gly Gin Asn Asp Gly Ala Ala Ala Val lie Val Thr 260 265 270

Thr Arg Ala Lys Ala Glu Glu Lys Gly Leu Arg Pro Val Met Arg Leu 275 280 285

Ala Gly Trp Ser Val Ala Ala Val Pro Pro Glu Thr Met Gly lie Gly 290 295 300

Pro Val Pro Ala Thr Lys Lys Val Leu Asp Arg Leu Gly Leu Thr Leu 305 310 315 320

Glu Asp lie Gly Ala lie Glu Leu Asn Glu Ala Phe Ala Ala Gin Ala 325 330 335

Leu Ser Val Leu Lys Glu Trp Asn lie Ser Trp Glu Asp Glu Arg Val 340 345 350

Asn Pro Leu Gly Ser Gly lie Ser Met Gly His Pro Val Gly Ala Thr 355 360 365

Gly Ala Arg Met Ala Val Thr Leu Ala His Arg Met Gin Arg Glu Asn 370 375 380

Thr Gin Tyr Gly Leu Ala Thr Met Cys lie Gly Gly Gly Gin Gly Leu 385 390 395 400

Ala Ala Val Phe Glu Lys Glu Asn 405 <210> 10 <211> 401 <212> PRT <213> Escherichia coli <400>

Met Arg Glu Ala Phe lie Cys Asp Gly lie Arg Thr Pro lie Gly Arg 15 10 15

Tyr Gly Gly Ala Leu Ser Ser Val Arg Ala Asp Asp Leu Ala Ala lie 20 25 30

Pro Leu Arg Glu Leu Leu Val Arg Asn Pro Arg Leu Asp Ala Glu Cys 35 40 45 lie Asp Asp Val lie Leu Gly Cys Ala Asn Gin Ala Gly Glu Asp Asn 50 55 60

Arg Asn Val Ala Arg Met Ala Thr Leu Leu Ala Gly Leu Pro Gin Ser 65 70 75 80

Val Ser Gly Thr Thr lie Asn Arg Leu Cys Gly Ser Gly Leu Asp Ala 85 90 95

Leu Gly Phe Ala Ala Arg Ala lie Lys Ala Gly Asp Gly Asp Leu Leu 14 201033370 100 105 110 lie Ala Gly Gly Val Glu Ser Met Ser Arg Ala Pro Phe Val Met Gly 115 120 125

Lys Ala Ala Ser Ala Phe Ser Arg Gin Ala Glu Met Phe Asp Thr Thr 130 135 140 lie Gly Trp Arg Phe Val Asn Pro Leu Met Ala Gin Gin Phe Gly Thr 145 150 155 160

Asp Ser Met Pro Glu Thr Ala Glu Asn Val Ala Glu Leu Leu Lys lie 165 170 175

Ser Arg Glu Asp Gin Asp Ser Phe Ala Leu Arg Ser Gin Gin Arg Thr 180 185 190

Ala Lys Ala Gin Ser Ser Gly lie Leu Ala Glu Glu lie Val Pro Val 195 200 205

Val Leu Lys Asn Lys Lys Gly Val Val Thr Glu lie Gin His Asp Glu 210 215 220

His Leu Arg Pro Glu Thr Thr Leu Glu Gin Leu Arg Gly Leu Lys Ala 225 230 235 240

Pro Phe Arg Ala Asn Gly Val lie Thr Ala Gly Asn Ala Ser Gly Val 245 250 255

Asn Asp Gly Ala Ala Ala Leu lie lie Ala Ser Glu Gin Met Ala Ala 260 265 270

Ala Gin Gly Leu Thr Pro Arg Ala Arg lie Val Ala Met Ala Thr Ala 275 280 285

Gly Val Glu Pro Arg Leu Met Gly Leu Gly Pro Val Pro Ala Thr Arg 290 295 300

Arg Val Leu Glu Arg Ala Gly Leu Ser lie His Asp Met Asp Val lie 305 310 315 320

Glu Leu Asn Glu Ala Phe Ala Ala Gin Ala Leu Gly Val Leu Arg Glu 325 330 335

Leu Gly Leu Pro Asp Asp Ala Pro His Val Asn Pro Asn Gly Gly Ala 340 345 350 lie Ala Leu Gly His Pro Leu Gly Met Ser Gly Ala Arg Leu Ala Leu 355 360 365

Ala Ala Ser His Glu Leu His Arg Arg Asn Gly Arg Tvr Ala Leu Cys 370 375 380

Thr Met Cys lie Gly Val Gly Gin Gly lie Ala Met lie Leu Glu Arg 385 390 395 400 15 201033370

Val

<210> 11 <211> 400 <212> PRT <213> Pseudomonas putida <400>

Met His Asp Val Phe lie Cys Asp Ala lie Arg Thr Pro lie Gly Arg 15 10 15

Phe Gly Gly Ala Leu Ala Ser Val Arg Ala Asp Asp Leu Ala Ala Val 20 25 30

Pro Leu Lys Ala Leu lie Glu Arg Asn Pro Gly Val Gin Trp Asp Gin 35 40 45

Val Asp Glu Val Phe Phe Gly Cys Ala Asn Gin Ala Gly Glu Asp Asn 50 55 60

Arg Asn Val Ala Arg Met Ala Leu Leu Leu Ala Gly Leu Pro Glu Ser 65 70 75 80 lie Pro Gly Val Thr Leu Asn Arg Leu Cys Ala Ser Gly Met Asp Ala 85 90 95

Val Gly Thr Ala Phe Arg Ala He Ala Ser Gly Glu Met Glu Leu Val 100 105 110 lie Ala Gly Gly Val Glu Ser Met Ser Arg Ala Pro Phe Val Met Gly 115 120 125

Lys Ala Glu Ser Ala Tyr Ser Arg Asn Met Lys Leu Glu Asp Thr Thr 130 135 140 lie Gly Trp Arg Phe lie Asn Pro Leu Met Lys Ser Gin Tyr Gly Val 145 150 155 160

Asp Ser Met Pro Glu Thr Ala Asp Asn Val Ala Asp Asp Tyr Gin Val 165 170 175

Ser Arg Ala Asp Gin Asp Ala Phe Ala Leu Arg Ser Gin Gin Lys Ala 180 185 190

Ala Ala Ala Gin Ala Ala Gly Phe Phe Ala Glu Glu lie Val Pro Val 195 200 205

Arg lie Ala His Lys Lys Gly Glu lie lie Val Glu Arg Asp Glu His 210 215 220

Leu Arg Pro Glu Thr Thr Leu Glu Ala Leu Thr Lys Leu Lys Pro Val 225 230 235 240

Asn Gly Pro Asp Lys Thr Val Thr Ala Gly Asn Ala Ser Gly Val Asn 245 250 255 16 201033370

Asp Gly Ala Ala Ala Met lie Leu Ala Ser Ala Ala Ala Val Lys Lys 260 265 270

His Gly Leu Thr Pro Arg Ala Arg Val Leu Gly Met Ala Ser Gly Gly 275 280 285

Val Ala Pro Arg Val Met Gly lie Gly Pro Val Pro Ala Val Arg Lys 290 295 300

Leu Thr Glu Arg Leu Gly lie Ala Val Ser Asp Phe Asp Val lie Glu 305 310 315 320

Leu Asn Glu Ala Phe Ala Ser Gin Gly Leu Ala Val Leu Arg Glu Leu 325 330 335

Gly Val Ala Asp Asp Ala Pro Gin Val Asn Pro Asn Gly Gly Ala He 340 345 350

Ala Leu Gly His Pro Leu Gly Met Ser Gly Ala Arg Leu Val Leu Thr 355 360 365

Ala Leu His Gin Leu Glu Lys Ser Gly Gly Arg Lys Gly Leu Ala Thr 370 375 380

Met Cys Val Gly Val Gly Gin Gly Leu Ala Leu Ala lie Glu Arg Val 385 390 395 400 <210> 12 <211> 394

<212> PRT <213> Ralstonia eutropha <400> 12

Met Thr Arg Glu Val Val Val Val Ser Gly Val Arg Thr Ala lie Gly 15 10 15

Thr Phe Gly Gly Ser Leu Lys Asp Val Ala Pro Ala Glu Leu Gly Ala 20 25 30

Leu Val Val Arg Glu Ala Leu Ala Arg Ala Gin Val Ser Gly Asp Asp 35 40 45

Val Gly His Val Val Phe Gly Asn Val lie Gin Thr Glu Pro Arg Asp 50 55 60

Met Tyr Leu Gly Arg Val Ala Ala Val Asn Gly Gly Val Thr lie Asn 65 70 75 80

Ala Pro Ala Leu Thr Val Asn Arg Leu Cys Gly Ser Gly Leu Gin Ala 85 90 95 lie Val Ser Ala Ala Gin Thr lie Leu Leu Gly Asp Thr Asp Val Ala 100 105 no lie Gly Gly Gly Ala Glu Ser Met Ser Arg Ala Pro Tyr Leu Ala Pro 115 120 125 17 201033370

Ala Ala Arg Trp Gly Ala Arg Met Gly Asp Ala Gly Leu Val Asp Met 130 135 140

Met Leu Gly Ala Leu His Asp Pro Phe His Arg lie His Met Gly Val 145 150 155 160

Thr Ala Glu Asn Val Ala Lys Glu Tyr Asp lie Ser Arg Ala Gin Gin 165 170 175

Asp Glu Ala Ala Leu Glu Ser His Arg Arg Ala Ser Ala Ala lie Lys 180 185 190

Ala Gly Tyr Phe Lys Asp Gin lie Val Pro Val Val Ser Lys Gly Arg 195 200 205

Lys Gly Asp Val Thr Phe Asp Thr Asp Glu His Val Arg His Asp Ala 210 215 220

Thr lie Asp Asp Met Thr Lys Leu Arg Pro Val Phe Val Lys Glu Asn 225 230 . 235 240

Gly Thr Val Thr Ala Gly Asn Ala Ser Gly Leu Asn Asp Ala Ala Ala 245 250 255

Ala Val Val Met Met Glu Arg Ala Glu Ala Glu Arg Arg Gly Leu Lys 260 265 270

Pro Leu Ala Arg Leu Val Ser Tyr Gly His Ala Gly Val Asp Pro Lys 275 280 285

Ala Met Gly lie Gly Pro Val Pro Ala Thr Lys lie Ala Leu Glu Arg 290 295 300

Ala Gly Leu Gin Val Ser Asp Leu Asp Val lie Glu Ala Asn Glu Ala 305 310 315 320 ❹

Phe Ala Ala Gin Ala Cys Ala Val Thr Lys Ala Leu Gly Leu Asp Pro 325 330 335

Ala Lys Val Asn Pro Asn Gly Ser Gly lie Ser Leu Gly His Pro lie 340 345 350

Gly Ala Thr Gly Ala Leu lie Thr Val Lys Ala Leu His Glu Leu Asn 355 360 365

Arg Val Gin Gly Arg Tyr Ala Leu Val Thr Met Cys lie Gly Gly Gly 370 375 380

Gin Gly lie Ala Ala lie Phe Glu Arg lie 385 390 <210> 13

<211> 406 <212> PRT <213> Rhodococcus sp. <400> 13 18 201033370

Met Ala Glu Val Phe Leu Val Asp Gly Ala Arg Thr Pro Gin Gly Arg 15 10 15

Tyr Gly Gly Ala Leu Ala Gly Val Arg Pro Asp Asp Leu Ala Gly Leu 20 25 30

Val Val Ala Glu Ala Ala Arg Arg Ala Gly lie Pro Gly Asp Ala Val 35 40 45

Asp Glu Val lie Leu Gly Ala Ala Asn Gin Ala Gly Glu Asp Asn Arg 50 55 60

Asp Val Ala Arg Met Ala Val Leu Leu Ala Gly Leu Pro Asp Ser Val 65 70 75 80

Pro Gly Tyr Thr Val Asn Arg Leu Cys Ala Ser Gly Leu Thr Ala Val 85 90 95 ❹

Ala Ser Ala Ala His Thr lie Arg Ser Gly Glu Ala Asp lie Val lie 100 105 110

Ala Gly Gly Val Glu Ser Met Thr Arg Ala Pro Trp Val Met Ala Lys 115 120 125

Pro Gly Thr Pro Trp Ala Arg Pro Gly Glu Val Ala Asp Thr Ser Leu 130 135 140

Gly Trp Arg Phe Thr Asn Pro Arg Phe Thr Ala Ala Asp Arg Asp Val 145 150 155 160

Pro Ala Gly Ala Gly Pro Asp Val Arg Lys Val Thr Leu Ser Met Gly 165 170 175

Glu Thr Ala Glu Glu Val Ala Ala Leu Glu Gly Val Thr Arg Ala Glu 180 185 190

Ser Asp Ala Phe Ala Leu Arg Ser Gin Glu Arg Ala lie Ala Ala Val 195 200 205

Asp Ala Gly Arg Phe Glu Arg Glu lie Val Pro Val Pro Val Arg Asp 210 215 220

Gly Glu Leu Ala Ala Asp Glu Thr Pro Arg Arg Gly Thr Thr Leu Glu 225 230 235 240

Lys Leu Gly Ser Leu Lys Pro Val Phe Arg Thr Gly Gly lie Val Thr 245 250 255

Ala Gly Ser Ser Ser Ser Leu Ser Asp Gly Ala Ala Ala Leu Val Val 260 265 270

Ala Ser Glu Ala Ala Val Glu Lys Tyr Gly Leu Thr Val Arg Gly Arg 275 280 285 lie Val Thr Ser Ala Ser Ala Gly lie Ala Pro Asn Val Met Gly Leu 290 295 300 19 201033370

Gly Pro Val Pro Ala Thr Arg Lys Ala Leu Ala Arg Ala Asn Trp Ser 305 310 315 320 lie Ser Asp Leu Gly Ala Ala Glu Leu Asn Glu Ala Phe Ala Ala Gin 325 330 335

Ser Leu Gly Val lie Arg Gin Leu Lys Leu Asp Glu Ser lie Val Asn 340 345 350

Ala Asp Gly Gly Ala lie Ala Leu Gly His Pro Leu Gly Cys Ser Gly 355 360 365

Ala Arg lie Leu Leu Thr Leu Leu Gly Arg Met Glu Arg Glu Gly Ala 370 375 380

Arg Arg Gly Leu Ala Thr Leu Cys Val Gly Val Gly Gin Gly Val Ala 385 390 395 400

Met Leu lie Glu Ala Pro 405 <210> 14 <211> 327

<212> PRT <213> Penicillium chrysogenum <400> 14

Met Ser Ala Pro Gly Ala Gly His Glu Phe Pro Ala Gin Glu Val Ser 1 5 10 15

Trp Gin Lys Arg Asp Val Leu Leu Phe Ala Asn Ser lie Gly Val Lys 20 25 30

Ala Asp Glu Leu His Phe Leu Tyr Glu Leu His Pro Asn Phe Ala Val 35 40 45

Phe Pro Thr Tyr Ser Leu lie Leu Arg Met Ser Lys Asn Thr Ser Asp 50 55 60

Phe Arg Thr lie Ala Leu Thr Pro Lys Pro Leu Ala Phe Lys Leu Thr 65 70 75 80

Asp Gin Glu Val Thr Asp Phe Tyr Ala Arg Gin Lys Ala Val His lie 85 90 95

Pro Gly Val Pro Asp Leu Asp His Arg His Gly Val Asp Gly Gin Arg 100 105 110

Lys lie Thr lie Leu Lys Pro Leu Pro Thr Thr Ser Ala Gly Arg Lys 115 120 125

Phe Glu Leu Arg Asn Lys Val lie Gly Val Tyr Asp Lys Gly Lys Pro 130 135 140

Gly Thr Val lie Glu Thr Glu Gin Ser lie Val Asp Lys Glu Ser Gly 145 150 155 160 20 201033370

Glu Val Tyr Ser Lys Val Val Ser Ser Gly Phe Leu Val Gly Gin Gly 165 170 175

Gly Trp Gly Gly Pro Lys Gly Pro Ser Thr Val Asn Tyr Ala Pro Pro 180 185 190

Glu Gly Arg Ala Pro Asp Ala Thr His Val Val Gin Ser Asn Ser Glu 195 200 205

Thr Ala His Leu Tyr Arg Leu Asn Gly Asp Tyr Asn Pro Leu His Ala 210 215 220

Thr Pro Glu Pro Gly Gin Lys Met Gly Phe Gly Gly lie lie lie His 225 230 235 240

Gly Leu Phe Ser Trp Asn Ser Ala Ala His Gly lie Leu Arg Glu Phe 245 250 255

Gly Gly Ser Asn Pro Ala Asn Met Lys Glu Phe Gin Ala Arg Phe Ala 260 265 270

Ser Pro Val Arg Pro Gly Asp Lys Leu Thr Thr Glu lie Trp Arg Met 275 280 285

Gly Asn lie Gin Asp Gly Tyr Glu Glu lie Arg Phe Val Thr Lys Asn 290 295 300

Asp Lys Gly Arg Val Val Leu Ser Asn Gly Arg Cys Leu Leu Lys Val 305 310 315 320

Thr Gly Val Lys Ser Lys Leu 325 <210> 15 <211> 901

<212> PRT <213> Penicillium chrysogenum <400> 15

Met Ser Glu Leu Arg Phe Asp Asn Gin Thr Val Val Val Thr Gly Ala 15 10 15

Gly Gly Gly Leu Gly Lys Ala Tyr Ala Leu Phe Phe Ala Ser Arg Gly 20 25 30

Ala Asn Val Val Val Asn Asp Leu Gly Ala Ser His Lys Gly Glu Gly 35 40 45

Lys Ser Gly Lys Ala Ala Asp Val Val Val Glu Glu lie Arg Ala Ala 50 55 60

Gly Gly Lys Ala Val Ala Asn Tyr Asp Ser Val Glu Asn Gly Asp Ala 65 70 75 80 lie lie Glu Thr Ala lie Lys Ala Phe Gly Arg lie Asp lie Leu Leu 85 90 95 21

Asn Asn Ala Gly lie Leu Arg Asp lie Ser Phe Lys Asn Met Lys Asp 100 105 110

Ala Asp Trp Asp Leu lie Asn Arg Val His Thr Tyr Gly Ala Tyr Lys 115 120 125

Cys Ala Arg Ala Ala Trp Pro His Phe Arg Lys Gin Lys Phe Gly Arg 130 135 140

Val lie Asn Thr Ala Ser Ala Ala Gly Leu Phe Gly Ser Phe Gly Gin 145 150 155 160

Ala Asn Tyr Ser Ala Ala Lys Leu Gly Gin Val Gly Phe Thr Glu Thr 165 170 175

Leu Ala Lys Glu Gly Ala Lys Tyr Asn lie lie Ala Asn Val lie Ala 180 185 190

Pro lie Ala Ala Ser Arg Met Thr Ala Thr Val Met Pro Pro Asp Val 195 200 205

Leu Glu Asn Leu Lys Pro Asp Trp Val Val Pro Leu Val Ala Ala Leu 210 215 220

Val His Ser Ser Asn Thr Thr Glu Thr Gly Gly lie Tyr Glu Val Gly 225 230 235 240

Gly Gly His Val Ala Lys Leu Arg Trp Glu Arg Ala Lys Gly Ala Leu 245 250 255

Leu Lys Thr Asp Asp Ser Leu Thr Pro Gly Ala lie Ala Arg Lys Trp 260 265 270

Asn Asp Val Asn Asp Phe Ser Gin Pro Glu Tyr Pro Thr Gly Pro Ala 275 280 285

Asp Phe Met Ala Phe Leu Glu Asp Gly Leu Lys Thr Pro Ser Ala Gin 290 295 300

Pro Gly Gin Glu Pro Asp Phe Lys Gly Arg Val Ala Leu Val Thr Gly 305 310 315 320

Gly Gly Ala Gly Leu Gly Arg Ala Tyr Cys Leu Gin Phe Ala Lys Leu 325 330 335

Gly Ala Ser Val Val Val Asn Asp Leu Val Asp Pro Glu Pro Val Val 340 345 350

Gin Glu lie Lys Lys Leu Gly Gly Lys Ala Val Gly Asn Lys Ala Ser 355 360 365

Cys Glu Asp Gly Asp Ala Val Val Lys Ser Ala lie Asp Ala Phe Gly 370 375 380

Arg lie Asp lie Leu Val Asn Asn Ala Gly lie Leu Arg Asp Lys Ala 385 390 395 400 22

Phe Thr Asn Met Asp Asp Asn Leu Trp Asn Ser Val Val Asn Val His 405 410 415

Leu Arg Gly Thr Tyr Lys Val Thr Lys Ala Ala Trp Pro Tyr Phe Leu 420 425 430

Lys Gin Lys Tyr Gly Arg Val Val Asn Thr Ala Ser Thr Ser Gly lie 435 440 445

Tyr Gly Asn Phe Gly Gin Ala Asn Tyr Ala Ala Ala Lys Leu Gly lie 450 455 460

Leu Gly Leu Ser Arg Thr Leu Ala Leu Glu Gly Ala Lys Tyr Asn He 465 470 475 480

Lys Val Asn Thr lie Ala Pro Asn Ala Gly Thr Asn Met Thr Arg Thr 485 490 495 lie Met Pro Glu Glu Met Val Gin Ala Phe Lys Pro Asp Tyr Val Ala 500 505 510

Pro Leu Val Val Leu Leu Cys Ser Asp Met Ala Pro Glu Pro Ser Thr 515 520 525

Lys Gly Leu Phe Glu Cys Gly Ser Gly Trp Phe Gly Arg Thr Arg Trp 530 535 540

Gin Arg Thr Gly Gly His Gly Phe Pro Val Asp Val Lys Leu Thr Pro 545 550 555 560

Glu Glu Val Val Arg Asn Trp Lys Gin lie lie Asn Phe Asp Asp Gly 565 570 575

Arg Ala Asp His Pro Glu Asp Gly Gin Ala Gly Ala Glu Lys lie Met 580 585 590

Ala Asn Met Ser Asn Arg Val His Gly Asp Thr Ser Thr Glu Asn Glu 595 600 605

Thr Leu Lys Asn lie Lys Lys Ala Lys Ala Leu Ser Ser Glu Gly Thr 610 615 620

Pro Phe Asn Tyr Glu Asp Arg Asp Val lie Leu Tyr Asn Leu Ser Leu 625 630 635 640

Gly Ala Lys Arg Thr Asp Leu Pro Leu Val Tyr Glu Asn Asn Asp Gin 645 650 655

Phe Gin Ala Leu Pro Ser Tyr Gly Val Val Pro Trp Phe Asn Thr Ala 660 665 670

Thr Pro Trp Asn Met Asp Asp Leu Val Lys Asp Phe Ser Pro Met Met 675 680 685

Leu Leu His Gly Glu Gin Tyr Met Glu Val Arg Lys Phe Pro lie Pro 23 690 695 700

Thr Thr Ala Asn Thr Leu Thr Tyr Pro Lys Leu lie Asp Val lie Asp 705 710 715 720

Lys Gly Asn Ala Ala lie Val Val Ala Gly Tyr Thr Thr Lys Asp Ala 725 730 735

Lys Thr Gly Glu Asp Leu Phe Tyr Asn Glu Ser Ser Val Phe lie Arg 740 745 750

Gly Ser Gly Gly Phe Gly Gly Ser Pro Lys Pro Thr Ala Val Arg Pro 755 760 765

Lys Ala Ala Thr Ala Ala Tyr Lys Ala Pro Gin Arg Gin Pro Asp Ala 770 775 780

Val Val Glu Glu Lys Thr Ser Glu Asp Gin Ala Ala Leu Tyr Arg Leu 785 790 795 800

Asn Gly Asp Arg Asn Pro Leu His lie Asp Pro Glu Phe Ser Lys Val 805 810 815

Gly Gly Phe Lys Thr Pro lie Leu His Gly Leu Cys Ser Leu Gly Val 820 825 830

Ser Ala Lys Ala Val Phe Ser Lys Tyr Gly Pro Tyr Lys Asn Leu Lys 835 840 845

Val Arg Phe Ala Gly Val Val Leu Pro Gly Gin Thr Leu Lys Thr Glu 850 855 860

Met Trp Lys Glu Gly Asn Thr Val Leu Phe Gin Ala Thr Val Val Glu 865 870 875 880

Thr Gly Lys Pro Ala lie Thr Gly Ala Gly Ala Glu Leu Leu Glu Gly 885 890 895

Ala Lys Ala Lys Leu 900 <210> 16 <211> 319

<212> PRT <213> Penicillium chrysogenum <400> 16

Met Leu Asn Ser Thr Leu Arg Phe Val Ser Leu Arg Arg Gly Pro Thr 15 10 15

Val Ala Arg Asn Phe Cys Ser Thr Ser Ala Val Arg Ala Ala Glu Val 20 25 30

Lys Ser Leu Gly Val Val Gly Ala Gly Gin Met Gly Leu Gly lie Ala 35 40 45

Leu Val Ala Ala Gin Lys Ala Asn Val Pro Val Thr Leu Val Asp Thr 24 50 55 60

Ser Gin Ala Ser Leu Asp Lys Gly Leu Lys Phe Ala Asp Lys Leu Leu 65 70 75 80

Glu Lys Asp Val Ala Lys Gin Arg Leu Thr Arg Glu Ala Ala Asp Glu 85 90 95

Ala Arg Gly Leu lie Ser Thr Ser Leu Thr Leu Asp Gly Leu Ser Ala 100 105 110

Val Asp Phe Val lie Glu Ala Val Pro Glu lie Pro Asp Leu Lys Thr 115 120 125

Lys lie Phe Ala Ser Leu Ala Gin lie Ala Pro Lys His Ala lie Leu 130 135 140

Ala Thr Asn Thr Ser Ser lie Ser lie Thr Lys lie Ala Ala Ala Thr 145 150 155 160

Ser Ala Asp Pro Thr Asp Leu Gin Ala Ser Ser Arg Val lie Ser Thr 165 170 175

His Phe Met Asn Pro Val Pro lie Gin Lys Gly Val Glu lie lie Arg 180 185 190

Gly Leu Gin Thr Ser Glu Glu Thr Met Asp Thr Ala lie Ala Phe Val 195 200 205

Gin Arg Met Gly Lys Val Ala Ser Val Ser Ala Asp Thr Pro Gly Phe 210 215 220

Leu Ala Asn Arg lie Leu Met Pro Tyr lie Asn Glu Ala Val lie Cys 225 230 235 240

Leu Glu Thr Gly Val Gly Gin Arg Glu Asp lie Asp Asn lie Met Lys 245 250 255

Thr Gly Thr Asn Val Pro Met Gly Pro Leu Val Leu Ala Asp Phe lie 260 265 270

Gly Leu Asp Thr Cys Leu Ala lie Met Asn Val Leu His Gin Asp Thr 275 280 285

Gly Asp Ser Lys Tyr Arg Pro Ala Gly Leu Leu Arg Arg Met Val Asp 290 295 300

Ala Gly Trp Leu Gly Lys Lys Ser Gly Lys Gly Phe Tyr Asp Tyr 305 310 315 <210> 17 <211> 335

<212> PRT <213> Penicillium chrysogenum <400> 17

Met Ala Thr Glu Tyr Pro His Arg lie Ala Leu Leu Gly Leu Gly Thr 25 1 5 10 15 lie Gly Leu Ser Met Leu Ala Met His Leu Arg Arg Pro Asp Thr Ser 20 25 30 lie Thr Val Tyr Asp Pro Arg Pro Asn Tyr Glu Ser Gin lie Arg Ser 35 40 45

Thr Leu Pro Ser Leu Leu Asp Cys Pro Glu Ser Thr Thr Leu lie Asp 50 55 60

Asp Leu Leu Arg Thr Ser Arg Leu Lys Leu Ala Ser Thr Val Ala Glu 65 70 75 80

Ala lie Gin Asn Ala Asn lie lie Gin Glu Gin Ser Pro Glu Val Thr 85 90 95

Ala Ser Lys Gin Ala Leu Trp Lys Glu Val Ala Gly Leu Thr Gly Pro 100 105 110

Asp Val His Leu Trp Ser Ser Ser Gly lie Ser Ala Ser Val Gin 115 120 125

Ala Ser Gly Cys Glu Ala Ala Asp Arg Val Ala Glu Arg Leu Leu Val 130 135 140

Ala His Pro Phe Asn Pro Pro His Leu Met Pro Leu lie Glu lie Val 145 150 155 160

Pro Gly Pro Glu Thr Asn Pro Glu Arg Val Glu Phe Val Arg Lys Tyr 165 170 175

Phe Gly Asp Val Pro Gly Pro Arg Ala Ser Gly Gly Asp Gin Ser Ala 180 185 190

Ser Gin His Tyr Arg Pro He Thr Leu His Lys Glu lie Pro Gly Phe 195 200 205

Val Gly Asn Arg Leu Ala Phe Ala Leu Leu Arg Glu Ala Cys Tyr Leu 210 215 220

Val Gly Glu Gly Val Val Ser Ala Lys Asp Leu Asp Ser Leu Val Thr 225 230 235 240

Ala Ser Leu Gly Pro Arg Trp Ala Gly Ser Gly Val Phe Glu Ser Tyr 245 250 255

His Ala Gly Gly Gly Glu Gly Gly lie Gly Ala Phe Leu Gin Lys Leu 260 265 270

Thr Pro Thr He Gin Asp Val Trp Gly Glu Leu Gly Gin lie Asp lie 275 280 285

Gin Gly Glu Gin Thr Trp Lys Asp Val Val Val Lys Gin Thr Glu Asp 290 295 300 26

Ala Tvr Gly Pro Tyr Thr Pro Glu Thr Arg Lys Lys Lys Glu Glu Met 305 310 315 320

Leu Arg Asp Val Val Glu Leu Gin 325

Lys Lys Lys Trp Gly Glu Leu 330 335

<210> 18 <211> 505 <212> PRT <213 > Acinetobacter sp. <400> 18

Ala He He Gly Val Gly Val Met 10 15

Met Thr His Pro lie Lys Lys lie 1 5

Gly Ser Gly lie Ala Gin lie Ala 20

Ala Gin Ser Gly His lie Thr Tyr 25 30

Leu Tyr Asp Ala Lys Ala Gly Ala 35 40

Ala Gin Gin Ala Lys Gin Gin Leu 45

Ala lie Thr Phe Gin Lys Leu Leu 50 55

Asp Lys Asn Lys lie Thr Thr Glu 60

Tyr Ala Asp Ala Ala Asn Ala Asn 65 70

Leu Leu lie Ala Asn Glu Leu His 75 80

Asp Leu Lys Asp Cys Asp Leu lie 85

Val Glu Ala lie Val Glu Arg Leu 90 95

Asp lie Lys Gin Ser Leu Met Ser 100

Gin Leu Glu Ala lie Val Pro Glu 105 110

Thr Thr lie Leu Ala Ser Asn Thr 115 120

Ser Ser Leu Ser lie Thr Ala lie 125

Ala Ser Asn Cys Lys His Pro Glu 130 135

Arg Val Ala Gly Tyr His Phe Phe 140

Asn Pro Val Pro Leu Met Lys Val 145 150

Val Glu Val lie Gin Gly Leu Lys 155 160

Thr Asp Pro Lys His lie Glu Thr 165

Leu Asn Gin Leu Ser Arg Val Leu 170 175

Gly His Arg Pro Val Val Ala Lys 180

Asp Thr Pro Gly Phe lie lie Asn 185 190

His Ala Gly Arg Ala Tyr Gly Thr 195 200

Glu Ala Leu Lys lie Leu Asn Glu 205

Asn Val Thr Asp lie Ser Glu lie 210 215

Asp Arg lie Leu Arg Asp Gly Val 220

Gly Phe Arg Met Gly Pro Phe Glu 225 230

Leu Met Asp Leu Thr Gly Leu Asp 235 240 27

Val Ser His Pro Val Met Glu Ser lie Tyr His Gin Tyr Tyr Glu Glu 245 250 255 Ala Arg Tyr Arg Pro Asn Ser Leu Thr Lys Gin Met Leu Glu Ala Lys 260 265 270 Gin Leu Gly Arg Lys Val Gly Gin Gly Phe Tyr Asp Tyr Arg Thr Gly 275 280 285 Ser Lys 290 Thr Gly Glu Thr Ser Ala Lys Val Ala Glu Arg Leu Thr Leu 295 300 Tyr Pro 305 Lys Val Trp lie Ala Ala Asp Phe Glu Asp Asp Lys Gin Leu 310 315 320 Leu lie Asn Tyr Leu Thr Thr His Asn lie Gin Leu Asp Val Gly Ala 325 330 335 Lys Pro Gin Ala Asp Ser Leu Cys Leu Leu Ala Cys Tyr Gly Glu Asp 340 345 350 Thr Thr His Ala Ala Leu Arg Leu Asn Val Asn Pro Ala His Ser Val 355 360 365 Ala lie 370 Asp Met Leu Tyr Gly lie Glu Lys His Arg Thr Leu Met Pro 375 380 Ser Leu 385 lie Thr Glu Val Thr Tyr Ser His Ala Ala His Ser lie Phe 390 395 400 Asn Leu Asp Gly Ala Met Val Ser Thr lie Gly Glu Ser lie Gly Phe 405 410 415 Val Ala Gin Arg lie Leu Ala Met Val lie Asn Leu Gly Cys Asp lie 420 425 430 Ala Gin Gin Ala lie Ala Ser Val Asp Asp lie Asn Ala Ala Val Arg 435 440 445 Leu Gly 450 Leu Gly Tyr Pro Phe Gly Pro lie Glu Trp Gly Asp Glu lie 455 460 Gly Ser 465 Asn Lys lie Leu Leu lie Leu Asn Arg lie Thr Ala Leu Thr 470 475 480 Ser Asp Pro Arg Tyr Arg Pro Ser Pro Trp Leu Gin Arg Arg Val Ala 485 490 495 Leu Asn Leu Pro Leu Thr Phe Thr Thr 500 505

<210> 19 <211> 282 <212> PRT <213> Clostridium kluyveri <400> 19 28 201033370

Met Ser lie 1

Lys Ser Val Ala Val Leu Gly Ser Gly Thr Met Ser Arg 5 10 15

Gly lie Val

Gin Ala Phe Ala Glu Ala Gly lie Asp Val lie lie Arg 20 25 30

Gly Arg Thr 35

Glu Gly Ser lie Gly Lys Gly Leu Ala Ala Val Lys Lys 40 45

Ala Tyr Asp 50

Lys Lys Val Ser Lys Gly Lys lie Ser Gin Glu Asp Ala 55 60

Asp Lys lie 65

Val Gly Arg Val Ser Thr Thr Thr Glu Leu Glu Lys Leu 70 75 80

Ala Asp Cys

Asp Leu lie lie Glu Ala Ala Ser Glu Asp Met Asn lie 85 90 95

Lys Lys Asp

Tyr Phe Gly Lys Leu Glu Glu lie Cys Lys Pro Glu Thr 100 105 110 碜 lie Phe Ala 115

Thr Asn Thr Ser Ser Leu Ser lie Thr Glu Val Ala Thr 120 125

Ala Thr Lys 130

Arg Pro Asp Lys Phe lie Gly Met His Phe Phe Asn Pro 135 140

Ala Asn Val 145

Met Lys Leu Val Glu lie lie Arg Gly Met Asn Thr Ser 150 155 160

Gin Glu Thr

Phe Asp lie lie Lys Glu Ala Ser lie Lys lie Gly Lys 165 170 175

Thr Pro Val

Glu Val Ala Glu Ala Pro Gly Phe Val Val Asn Lys lie 180 185 190

Leu Val Pro 195

Met lie Asn Glu Ala Val Gly lie Leu Ala Glu Gly lie 200 205

Ala Ser Ala 210

Glu Asp lie Asp Thr Ala Met Lys Leu Gly Ala Asn His 215 220

Pro Met Gly 225

Pro Leu Ala Leu Gly Asp Leu lie Gly Leu Asp Val Val 230 235 240

Leu Ala Val

Met Asp Val Leu Tyr Ser Glu Thr Gly Asp Ser Lys Tyr 245 250 255

Arg Ala His

Thr Leu Leu Arg Lys Tyr Val Arg Ala Gly Trp Leu Gly 260 265 270

Arg Lys Ser 275

Gly Lys Gly Phe Phe Ala Tyr 280

<210> 20 <211> 319 <212> PRT 29 <213> Clostridium kluyveri <400> 20

Met Asp lie Lys Asn Val Ala Val Leu Gly Thr Gly Thr Met Gly Asn 15 10 15

Gly lie Val Gin Leu Cys Ala Glu Ser Gly Leu Asn Val Asn Met Phe 20 25 30

Gly Arg Thr Asp Ala Ser Leu Glu Arg Gly Phe Thr Ser lie Lys Thr 35 40 45

Ser Leu Lys Asn Leu Glu Glu Lys Gly Lys lie Lys Thr Asn lie Ser 50 55 60

Lys Glu lie Leu Lys Arg lie Lys Gly Val Lys Thr lie Glu Glu Ala 65 70 75 80

Val Glu Gly Val Asp Phe Val lie Glu Cys lie Ala Glu Asp Leu Glu 85 90 95

Leu Lys Gin Glu Val Phe Ser Lys Leu Asp Glu lie Cys Ala Pro Glu 100 105 110

Val lie Leu Ala Ser Asn Thr Ser Gly Leu Ser Pro Thr Asp lie Ala 115 120 125 lie Asn Thr Lys His Pro Glu Arg Val Val lie Ala His Phe Trp Asn 130 135 140

Pro Pro Gin Phe lie Pro Leu Val Glu Val Val Pro Gly Lys His Thr 145 150 155 160

Asp Ser Lys Thr Val Asp lie Thr Met Asp Trp lie Glu His lie Gly 165 170 175

Lys Lys Gly Val Lys Met Arg Lys Glu Cys Leu Gly Phe lie Gly Asn 180 185 190

Arg Leu Gin Leu Ala Leu Leu Arg Glu Ala Leu Tyr lie Val Glu Gin 195 200 205

Gly Phe Ala Thr Ala Glu Glu Val Asp Lys Ala lie Glu Tyr Gly His 210 215 220

Gly Arg Arg Leu Pro Val Thr Gly Pro lie Cys Ser Ala Asp Leu Gly 225 230 235 240

Gly Leu Asp lie Phe Asn Asn lie Ser Ser Tyr Leu Phe Lys Asp Leu 245 250 255

Cys Asn Asp Thr Glu Pro Ser Lys Leu Leu Lys Ser Lys Val Asp Gly 260 265 270

Gly Asn Leu Gly Ser Lys Thr Gly Lys Gly Phe Tyr Asn Trp Thr Pro 275 280 285 30

Glu Phe Leu Gin Lys Lys Gin Asn Glu Arg lie Gin Leu Leu Met Asp 290 295 300

Phe Leu Glu Lys Asp Lys Asn Asp Lys Ser lie Glu Arg Asn lie 305 310 315 <210> 21 <211> 475

<212> PRT <213> Escherichia coli <400> 21

Met Met lie Asn Val Gin Thr Val Ala Val lie Gly Ser Gly Thr Met 15 10 15

Gly Ala Gly lie Ala Glu Val Ala Ala Ser His Gly His Gin Val Leu 20 25 30

Leu Tyr Asp lie Ser Ala Glu Ala Leu Thr Arg Ala lie Asp Gly lie 35 40 45

His Ala Arg Leu Asn Ser Arg Val Thr Arg Gly Lys Leu Thr Ala Glu 50 55 60

Thr Cys Glu Arg Thr Leu Lys Arg Leu lie Pro Val Thr Asp lie His 65 70 75 80

Ala Leu Ala Ala Ala Asp Leu Val lie Glu Ala Ala Ser Glu Arg Leu 85 90 95

Glu Val Lys Lys Ala Leu Phe Ala Gin Leu Ala Glu Val Cys Pro Pro 100 105 110

Gin Thr Leu Leu Thr Thr Asn Thr Ser Ser lie Ser lie Thr Ala lie 115 120 125

Ala Ala Glu lie Lys Asn Pro Glu Arg Val Ala Gly Leu His Phe Phe 130 135 140

Asn Pro Ala Pro Val Met Lys Leu Val Glu Val Val Ser Gly Leu Ala 145 150 155 160

Thr Ala Ala Glu Val Val Glu Gin Leu Cys Glu Leu Thr Leu Ser Trp 165 170 175

Gly Lys Gin Pro Val Arg Cys His Ser Thr Pro Gly Phe lie Val Asn 180 185 190

Arg Val Ala Arg Pro Tyr Tyr Ser Glu Ala Trp Arg Ala Leu Glu Glu 195 200 205

Gin Val Ala Ala Pro Glu Val lie Asp Ala Ala Leu Arg Asp Gly Ala 210 215 220

Gly Phe Pro Met Gly Pro Leu Glu Leu Thr Asp Leu lie Gly Gin Asp 225 230 235 240 31

Val Asn Phe Ala Val Thr Cys Ser Val Phe Asn Ala 245 250 Phe Trp Gin Glu 255 Arg Arg Phe Leu Pro Ser Leu Val Gin Gin Glu Leu 260 265 Val lie Gly Gly 270 Arg Leu Gly Lys Lys Ser Gly Leu Gly Val Tyr Asp 275 280 Trp Arg Ala Glu 285 Arg Glu Ala Val Val Gly Leu Glu Ala Val Ser Asp 290 295 300 Ser Phe Ser Pro Met Lys Val Glu Lys Lys Ser Asp Gly Val Thr Glu 305 310 315 lie Asp Asp Val 320 Leu Leu He Glu Thr Gin Gly Glu Thr Ala Gin Ala 325 330 Leu Ala lie Arg 335 Leu Ala Arg Pro Val Val Val lie Asp Lys Met Ala 340 345 Gly Lys Val Val 350 Thr lie Ala Ala Ala Ala Val Asn Pro Asp Ser Ala 355 360 Thr Arg Lys Ala 365 lie Tyr Tyr Leu Gin Gin Gin Gly Lys Thr Val Leu 370 375 380 Gin lie Ala Asp Tyr Pro Gly Met Leu lie Trp Arg Thr Val Ala Met 385 390 395 lie lie Asn Glu 400

Ala Leu Asp Ala Leu Gin Lys Gly Val Ala Ser Glu Gin Asp lie Asp 405 410 415 Thr Ala Met Arg Leu Gly Val Asn Tyr Pro Tyr Gly 420 425 Pro Leu Ala Trp 430 Gly Ala Gin Leu Gly Trp Gin Arg lie Leu Arg Leu 435 440 Leu Glu Asn Leu 445 Gin His His Tyr Gly Glu Glu Arg Tyr Arg Pro Cys 450 455 460 Gin Arg Ala Leu Leu Glu Ser Gly Tyr Glu Ser 465 470 475 Ser Leu Leu Arg <210> 22 <211> 320 <212> PRT <213> Euglena gracilis <400> 22 Met Met Ser Arg Asn Val Phe Thr Cys Cys Arg Ala 15 10 lie Arg Thr Tyr 15 Ala Thr Ala Pro Gly Leu Ser Thr Val Gly Val Val 20 25 Gly Met Gly Ala 30 32

Met Gly His Gly lie Ala Gin Met Thr Ala Ala Ala Gly Tyr Lys Val 35 40 45

Val Ala Val Asp lie Asp Ala Asn Met Leu Ser Lys Gly lie Lys Ala 50 55 60

Val Glu Asp Ser Leu Ser Lys Val Ala Ala Lys Ala Val Lys Asp Gly 65 70 75 80

Lys Ala Asp Lys Ala Thr Ala Glu Lys Asn Ala Ala Asp Val Arg Ser 85 90 95

Arg lie Thr Thr Ser Gly Asp lie Gly Ala Leu Ser Ser Cys Asp Leu 100 105 110

Val lie Glu Ser lie lie Glu Asp Leu Asn lie Lys Lys Lys Phe Phe 115 120 125

Ala Asp Leu Gly Lys Val Ala Gly Ala Asn Ala lie Leu Ala Ser Asn 130 135 140

Thr Ser Ser Phe Pro lie Thr Gin Leu Gly Glu Ala Ser Gly Arg Thr 145 150 155 160

Ser Asn Phe Leu Gly Leu His Phe Phe Asn Pro Val Gin Met Met Lys 165 170 175

Leu Val Glu Val He Lys Thr Lys Asp Thr Lys Glu Asp Val Tyr Lys 180 185 190

Leu Gly Phe Ala Phe Ser Lys Ser lie Gly Lys Glu Pro Val Ala Cys 195 200 205

Gly Asp Thr Pro Gly Phe lie Val Asn Arg Leu Leu Val Pro Phe Leu 210 215 220

Ala Gin Gly Leu Leu Met Leu Asp Arg Gly Val Ala Ser Val Gin Asp 225 230 235 240 lie Asp Val Ala Met Met Tyr Gly Ala Gly Met Pro Met Gly Pro Leu 245 250 255

Thr Leu Ala Asp Tyr Val Gly Leu Asp Val Cys Met His lie Leu Glu 260 265 270

Gly Trp Thr Ser Gin Tyr Pro Asn Glu Pro Ala Phe Val lie Pro Gin 275 280 285

Pro Leu Lys Ala Lys Val Ala Ala Gly Lys Leu Gly Arg Lys Thr Gly 290 295 300

Glu Gly Phe Trp Lys Trp Glu Gly Asp Lys Pro Val Ala Pro Ala Ala 305 310 315 320 <210> 23 <211> 505 33

<212> PRT <213> Pseudomonas putida <400> 23

Met Ala Ala Leu Ala Ser Asn Val Gin Val Ala Val lie Gly Ala Gly 15 10 15

Ala Met Gly Ala Gly lie Ala Gin Val Ala Ala Gin Ala Gly His Pro 20 25 30

Val Lys Leu Tyr Asp Asn Arg Pro Gly Ala Ala Ala Gin Ala Val Thr 35 40 45

Gly lie Asp Arg Gin Leu Ala Arg Leu Val Asp Lys Gly Lys Leu Leu 50 55 60

Ala Ala Glu Arg Glu Thr lie Asn 65 70

Ala Arg Leu Cys Pro Val Asp Thr 75 80

Leu Glu Ala Leu Ala Asp Ala Gly 85

Leu Val He Glu Ala He Val Glu 90 95

Asn Leu Gin Val Lys Gin Ala Leu 100

Phe Ser Gin Leu Glu Thr Leu Cys 105 110

Ala Ala Asp Cys lie Leu Ala Ser 115 120

Asn Thr Ser Ser Leu Ser lie Thr 125

Ser Leu Ala Ala Gly Leu Glu Arg Pro Gin His Val Val Gly Met His 130 135 140

Phe Phe Asn Pro Ala Pro Leu Met Ala Leu Val Glu Val Val Ser Gly 145 150 155 160

Leu Ala Thr Asp Pro Ala Val Ala Ala Cys lie Tyr Ala Thr Ala Gin 165 170 175

Ala Trp Gly Lys Gin Pro Val His Ala Arg Ser Thr Pro Gly Phe lie 180 185 190

Val Asn Arg Val Ala Arg Pro Phe Tyr Ala Glu Ser Leu Arg Leu Leu 195 200 205

Gin Glu Gly Ala Ala Asp Cys Ala Ser Leu Asp Ala Leu Met Arg Asp 210 215 220

Ser Gly Gly Phe Arg Met Gly Ala Phe Glu Leu Thr Asp Leu lie Gly 225 230 235 240

His Asp Val Asn Tyr Ala Val Thr Cys Ser Val Phe Asp Ala Phe Tyr 245 250 255

Gly Asp Phe Arg Phe Gin Pro Ser Leu Val Gin Lys Glu Leu Val Asp 260 265 270

Ala Gly His Leu Gly Arg Lys Thr Gly Gin Gly Phe Tyr Arg Tyr Ala 275 280 285 34

Glu Gly Val Glu Arg Pro Gin Pro Ala Glu Leu His Ser Ser Ala Cys 290 295 300

Ala Glu Ala Cys Val Val Glu Gly Asn Leu Gly Val Met Gin Pro Leu 305 310 315 320

Val Glu Arg Leu Arg Gin Ser Gly lie Ala Val Thr Gin Arg Ala Gly 325 330 335

Ser Gly Leu lie Gin Val Gly Asp Ala Thr Leu Ala Leu Ser Asp Gly 340 345 350

Arg Leu Ala Ser Gin Arg Ala Arg Glu Asp Gly Leu Arg Asn Leu Val 355 360 365

Leu Leu Asp Leu Ala Leu Asp Tyr Ser Ser Ala Thr Arg lie Ala lie 370 375 380

Ser Trp Ser Ala Asp Thr Ser Asp Ser Ala Arg Asp Gin Ala Val Ala 385 390 395 400

Leu Leu Gin Arg Ala Gly Leu Lys Val Thr Gly Val Ala Asp Leu Pro 405 410 415

Gly Leu Val Val Leu Arg Thr Val Ala Met Leu Ala Asn Glu Ala Ala 420 425 430

Asp Ala Val Leu Gin Gly Val Gly Ser Ala Ala Asp lie Asp Leu Ala 435 440 445

Met Arg Ala Gly Val Asn Tyr Pro Cys Gly Pro Leu Ala Trp Ala Ala 450 455 460

Asn lie Gly lie Ala His Thr Leu Arg Val Leu Asp Asn Leu Gin Cys 465 470 475 480

Ser Tyr Gly Glu Ser Arg Tyr Arg Pro Ser Leu Leu Leu Arg Arg Cys 485 490 495

Glu Ala Lys Gly Gly Thr Leu His Asp 500 505

<210> 24 <211> 246 <212> PRT <213> Ralstonia eutropha <400>

Met Thr Gin Arg lie Ala Tyr Val Thr Gly Gly Met Gly Gly lie Gly 1 5 10 15

Thr Ala lie Cys Gin Arg Leu Ala Lys Asp Gly Phe Arg Val Val Ala 20 25 30

Gly Cys Gly Pro Asn Ser Pro Arg Arg Glu Lys Trp Leu Glu Gin Gin 35 40 45 35

Lys Ala Leu Gly Phe Asp Phe lie Ala 50 55 Ser Glu Gly Asn Val Ala Asp 60 Trp Asp Ser Thr Lys Thr Ala Phe Asp 65 70 Lys Val Lys Ser Glu Val Gly 75 80 Glu Val Asp Val Leu lie Asn Asn Ala 85 Gly Lie Thr Arg Asp Val Val 90 95 Phe Arg Lys Met Thr Arg Ala Asp Trp 100 105 Asp Ala Val lie Asp Thr Asn 110 Leu Thr Ser Leu Phe Asn Val Thr Lys 115 120 Gin Val lie Asp Gly Met Ala 125 Asp Arg Gly Trp Gly Arg lie Val Asn 130 135 lie Ser Ser Val Asn Gly Gin 140 Lys Gly Gin Phe Gly Gin Thr Asn Tyr 145 150 Ser Thr Ala Lys Ala Gly Leu 155 160 His Gly Phe Thr Met Ala Leu Ala Gin 165 Glu Val Ala Thr Lys Gly Val 170 175 Thr Val Asn Thr Val Ser Pro Gly Tyr 180 185 lie Ala Thr Asp Met Val Lys 190 Ala lie Arg Gin Asp Val Leu Asp Lys 195 200 lie Val Ala Thr lie Pro Val 205 Lys Arg Leu Gly Leu Pro Glu Glu Lie 210 215 Ala Ser lie Cys Ala Trp Leu 220 Ser Ser Glu Glu Ser Gly Phe Ser Thr 225 230 Gly Gly Leu His Met Gly 245 Gly Ala Asp Phe Ser Leu Asn 235 240 <210> 25 <211> 284 <212> PRT <213> Rhodococcus sp. <400> 25 Met Thr Ser Thr Ala Pro Lys Asn Val 1 5 Gly Val Val Gly Gly Gly Arg 10 15 Met Gly Ala Gly lie Ala Gin Val Phe 20 25 Ala Thr Leu Gly Ser Thr Val 30 lie lie Ala Glu Ser Gly Asp Arg Glu 35 40 Ala Ala Val Lys Arg Val Ser 45 Asp Gly Leu Asp Arg Ala His Glu Arg 50 55 Gly Lys Leu Gly Asp Val Asp 60 36 201033370

Pro Ala Thr lie Leu Gly Arg Val Ser Thr Val Ala Ala Pro Asp Ala 65 70 75 80

Leu Pro Pro Ala Leu Asp Leu Val Val Glu Ala Val Pro Glu Leu Val 85 90 95

Asp Leu Lys Leu Ser Val Leu Ser Leu Val Glu Lys Thr Val Ser Pro 100 105 110

Thr Thr Val lie Ala Ser Asn Thr Ser Ser lie Ser lie Ala Glu Leu 115 120 125

Gly Ser Ala Leu Gly Asp Pro Ala Arg Leu lie Gly Met His Phe Phe 130 135 140

Asn Pro Val Pro Ala Ser Ser Leu Val Glu lie Val Arg Ala Pro Ala 145 150 155 160

Thr Asp Ala Gly Val Val Glu Lys Val Arg Glu Trp Val Ala Gin Leu 165 170 175

Gly Lys Thr Glu Val Leu Val Asn Asp Ser Pro Gly Phe Ala Thr Ser 180 185 190

Arg Leu Gly Val Cys Leu Gly Leu Glu Ala lie Arg Met Leu Glu Glu 195 200 205

Gly Val Ala Asp Ala Glu Ser lie Asp Arg Ala Met Glu Leu Gly Tyr 210 215 220

Arg His Pro Met Gly Pro Leu Arg Ser Thr Asp Leu Val Gly Leu Asp 225 230 235 240

Val Arg Leu Ala lie Ala Glu His Leu Ala Lys Thr Leu Gly Asp Arg 245 250 255

Phe Ala Pro Pro Ala Leu Leu Arg Glu Lys Val Ala Lys Gly Glu Leu 260 265 270

Gly Arg Lys Thr Gly Gin Gly Phe Phe Thr Trp Ser 275 280 <210> 26 <211> 699

<212> PRT <213 > Rhodopseudomonas palustris <400> 26

Met Ser Glu Val Val Thr Arg Ala Thr Gin Asp Gin Val Ala lie Val 15 10 15

Thr Val Asp Ser Pro Pro Val Asn Ala Leu Ser Ala Ala Val Arg Arg 20 25 30

Gly lie Leu Glu Asn Val Asn Ala Ala Val Ala Asp Pro Ala Val Gin 35 40 45 37

Ala lie Val Leu Val Cys Ala Gly 50 55

Arg Thr Phe lie Ala Gly Ala Asp 60 lie Thr Glu Phe Gly Lys Pro Pro 65 70

Gin Pro Pro Ala Leu Asn Asp Val 75 80 lie Ala Ala Leu Glu Asn Ser Pro 85

Lys Pro Thr lie Ala Ala lie His 90 95

Gly Thr Ala Leu Gly Gly Gly Leu 100

Glu Val Ala Leu Gly Cys His Phe 105 110

Arg Val Ala Val Lys Glu Ala Lys 115 120

Leu Gly Leu Pro Glu Val Lys Leu 125

Gly Leu Leu Pro Gly Ala Gly Gly 130 135

Thr Gin Arg Leu Pro Arg Ala Val 140

Gly Pro Glu Leu Ala Val Gin Met 145 150

He Val Gly Gly Ser Pro lie Gly 155 160

Ala Ala Glu Ala Leu Lys His Gly Leu Val Glu Glu Val Val Glu Asn 165 170 175

Leu Val Ala Gly Ala Val Ala Phe Ala Lys Lys Val Leu Ala Glu Lys 180 185 190

Arg Pro Leu Arg Arg Leu Arg Asp Asp Asp Ser Lys Leu Ala Ala Ala 195 200 205

Lys Ala Asp Arg Ser lie Phe Thr Asn Ala Val Ala Ala Met Thr Lys 210 215 220

Lys Ala Arg Gly Leu Glu Ala Pro Phe Ala Cys Ala Asp Ala lie Gly 225 230 235 240

Ala Ala lie Asp Leu Pro Phe Glu Glu Gly Leu Lys Lys Glu Arg Glu 245 250 255

Gly Phe Met Lys Leu Val Val Ser Asp Gin Ser Lys Ala Gin Arg Tyr 260 265 270

Ala Phe Phe Ala Glu Arg Glu Ala Ala Lys Val Asp Gly Val Pro Asp 275 280 285

Gly Thr Lys Pro Arg Pro Val Ser Arg Val Ala lie lie Gly Ala Gly 290 295 300

Thr Met Gly Gly Gly lie Ala Met Ser Phe Ala Asn Ala Gly lie Pro 305 310 315 320

Val Thr Leu lie Glu Thr Gly Glu Glu Gin Leu Lys Arg Gly Leu Gly 325 330 335 lie Met Gin Lys Asn Trp Glu Ala Thr Ala Ala Arg Gly Gly Leu Pro 38 201033370 340 345 350

Pro Asp Ala Pro Ala Lys Arg Met Ala Leu lie Thr Gly Leu Val Gly 355 360 365

Leu Glu Asn Val Lys Asp Ala Asp Leu lie lie Glu Ala Val Phe Glu 370 375 380

Thr Met Ala Val Lys Lys Glu Val Phe Thr Ala Val Asp Ala His Ala 385 390 395 400

Lys Pro Gly Ala Val Leu Ala Ser Asn Thr Ser Tyr Leu Ser lie Asp 405 410 415

Glu lie Ala Ala Thr Thr Lys Arg Pro Gin Asp Val Leu Gly Met His 420 425 430

Phe Phe Ser Pro Ala Asn Val Met Lys Leu Cys Glu lie Val Arg Gly 435 440 445

Ala Lys Thr Ala Pro Asp Ala Leu Leu Thr Ala Val Ser lie Ala Lys 450 455 460

Lys lie Ala Lys Val Pro Val Val Val Gly Val Cys Asp Gly Phe Val 465 470 475 480

Gly Asn Arg Met Leu Ala Ala Arg Ser Lys Gin Ser Glu Lys Leu Leu 485 490 495

Phe Glu Gly Ala Leu Pro Gin Gin Val Asp Ala Val Val Thr Lys Phe 500 505 510

Gly Met Pro Met Gly Pro Phe Ala Met Gly Asp Leu Ala Gly Leu Asp 515 520 525 ❹ lie Gly Trp Arg Ser Arg Lys Asp Arg Gly lie Lys Ser Glu lie Ala 530 535 540

Asp Ala Leu Cys Glu Ala Gly Arg Phe Gly Gin Lys Thr Gly Lys Gly 545 550 555 560

Tyr Tyr Lys Tyr Glu Gin Gly Ser Arg Ala Pro Met Pro Asp Pro Glu 565 570 575

Val Glu Thr Leu lie Asn Asp Thr Leu Ala Lys Leu Gly Leu Lys Arg 580 585 590

Arg Asp lie Thr Asp Glu Glu lie Leu Glu Arg Met Val Tyr Pro Met 595 600 605 lie Asn Glu Gly Ala Arg lie Leu Glu Glu Lys lie Ala Ala Arg Pro 610 615 620

Ser Asp lie Asp Val Val Trp Leu Tyr Gly Tyr Gly Trp Pro lie Tyr 625 630 635 640 39

Arg Gly Gly Pro Met His Tyr Ala Asp Ser Val Gly Leu Lys His lie 645 650 655

Ala Glu Arg Leu Ser Ala Tyr Ala Lys Ala Thr Asn Asp Pro Ser Leu 660 665 670

Glu Pro Ala Pro Leu Leu Ala Arg Leu Ala Ala Glu Gly Lys Thr Phe 675 680 685

Ala Ser Leu Thr Gin Pro Ser Lys Ala Ala Ala 690 695 <210> 27 <211> 278 <212> PRT <213> Penicillium chrysogenum <400>

Met Ser Leu Leu Thr Thr Tyr Thr Thr Gin Asn Thr Thr Lys lie Ala 15 10 15

His Leu Thr lie Ser His Ala Gin Lys Leu Asn Ser Leu Thr Thr Pro 20 25 30

Leu Leu Thr Ser Leu Thr Gin Thr Leu Ser Lys Leu Ser Asn Thr Asn 35 40 45

Leu His Ala lie Thr Leu Thr Gly Ala Gly Gin Lys Ser Phe lie Gly 50 55 60

Gly Ala Asp Leu Asn Glu Leu Ser Thr Leu Arg Asn Ala Pro Thr Ala 65 70 75 80

Arg Lys Phe lie Thr Ser Val His Glu Thr Cys Thr Ala lie Arg Thr 85 90 95

Cys Pro Val Pro Val lie Ala Arg lie Asn Gly Phe Ala Leu Gly Ala 100 105 no

Gly Leu Glu lie Ala Ala Ala Cys Asp Leu Arg Val Ala Ala Lys Gly 115 120 125

Ala Val Phe Gly Met Pro Glu Val Arg Leu Gly lie Pro Ser Val Val 130 135 140

Glu Ala Ala Leu Leu Pro Gly Leu lie Gly Trp Gly Arg Thr Arg Gin 145 150 155 160

Leu Leu Leu Leu Gly Gly Met lie Ser Ala Ser Glu Ala Leu Arg Trp 165 170 175

Gly Leu Val Glu Arg Val Val Glu Asp Glu Glu Leu Asp Leu Ala Val 180 185 190

Ala Glu Trp Thr Ser Glu lie Gly Arg Asn Gly Pro Leu Ala Val Arg 195 200 205 40 201033370

Arg Gin Lys Ala Leu lie Ser Arg Trp Glu Glu Leu Ser Leu Ala Gly 210 215 220

Gly lie Glu Ala Gly lie Glu Ala Phe Gly Glu Cys Phe Asp Gly Asp 225 230 235 240

Cys Gly Thr Glu Pro Gly Arg Met lie Gly Glu Phe Phe Arg Glu Lys 245 250 255

Glu Arg Ser Lys Ala Arg lie Glu Ala Arg Asn Lys Val Val Ala Gly 260 265 270

Ser Lys Gly Glu Glu Gly 275

<210> 28 <211> 244 <212> PRT

<213> Penicillium chrysogenum <400> 28

Met Thr Glu lie Pro Thr Phe His Asn Leu Ser Leu Glu Arg His Gly 15 10 15

Asn Val Phe Val Leu Thr Met Gin Lys Pro Pro Glu Asn Arg Leu Asn 20 25 30

Ser Ser Tyr Cys Gin Glu Met lie Arg Ala Tyr Arg Ser Val Glu Arg 35 40 45 lie Leu Gly Ser Asp Ser Glu Gly Ala Val lie Thr Arg Gly Asn Asp 50 55 60

Ala Lys Phe Trp Cys Thr Gly Leu Glu Leu Asp Glu Ser Asp Ser Asn 65 70 75 80

Pro Phe Ala Asn Thr Asp Gly Phe Tyr Pro Leu lie His Thr lie Leu 85 90 95

Asp Phe Pro Phe Pro Thr Val Ala Leu Leu Thr Gly His Thr Phe Gly 100 105 110

Gly Ala Cys Pro Leu Ala Leu Ala His Asp Tyr Arg lie Met Asn Ser 115 120 125

Arg Arg Gly Phe lie Ser Met Pro Pro Val Asn Leu Gly Leu His Phe 130 135 140

Asp Gly lie Gly Ser Leu Pro Arg Leu Lys Leu Arg Pro Gin Val Ala 145 150 155 160

Arg Lys Met Leu Leu Glu Ala His Arg Trp Thr Gly Pro Glu Ala Leu 165 170 175

Glu Asp Gly lie Val Asp Ala Val Ala Glu Pro Glu Asp Met Leu Asn 180 185 190 41

Val Ala Leu Glu Leu Gly Ala Lys 195 200 Trp Ala Pro Lys Ala Lys Met Gly 205 Val Tyr Ala Leu Leu Arg Gin Glu 210 215 Leu Trp Gly Asp Ala lie Lys Lys 220 Phe Gin Arg lie Ser Tyr Val His 225 230 Lys Val Lys lie Ser Arg Val Thr Ser Ala Pro Ala 235 240 <210> 29 <211> 300 <212> PRT <213> Penicillium chrysogenum <400> 29 Met Ala Pro Ser Asp Arg Leu Thr 1 5 Gin Val Asn Glu His Leu Asn Tyr 10 15 Pro Ala Gly Leu Leu Ala Gly Gin 20 Val Ala lie lie Thr Gly Ala Gly 25 30 Gin Gly lie Gly Ala Glu Ala Ala 35 40 Arg Leu Phe Ala Asn Glu Gly Ala 45 Lys Val Val Val Ala Asp lie Asp 50 55 Ser Lys Lys Ala Asn Ala Val Ala 60 Asp Ala lie Asn Ser Ala Lys Ala 65 70 Gly Arg Ala Leu Ala Val Val Gly 75 80 Asp Val Leu Asp Ser Asn Tyr lie 85 Thr Glu Leu Val Lys Lys Thr Ala 90 95 Glu Phe Gly Asn Gly Lys lie His 100 lie lie Val Asn Asn Ala Gly Phe 105 110 Thr Trp Asp Gly Val lie His Lys 115 120 Met Thr Asp Lys Gin Trp Glu Thr 125 Met Leu Ala Val His Asn Thr Ala 130 135 Pro Phe G In Leu Val Arg Ala Ala 140 Ala Pro Tyr Phe Arg Val Lys Asp 145 150 Gin Glu Pro Arg Val Val lie Asn 155 160 lie Ser Ser Thr Ser Gly Val His 165 Gly Asn Ala Gly Gin Ala Asn Tyr 170 175 Ala Val Ala Lys Ala Gly Val Val 180 Gly Leu Thr Arg Thr lie Ala Lys 185 190 Glu Trp Gly Pro Ser Phe Gly Val Arg Ser Asn Thr lie Ala Phe Gly 195 200 205 42 201033370

Phe Val Thr Thr Arg Leu Thr Ala Ala Lys Glu Glu Gly Ala Phe lie 210 215 220

Thr Thr Pro Asp Gly Thr Lys Val Ala Leu Gly lie Pro Gly Lys Gin 225 230 235 240

Leu Ala Thr Lys Lys Gly Ser Ala Asp Gin Glu Lys Lys Ala Ala Pro 245 250 255

Thr Tyr Pro Asp lie Pro Leu Gly Arg Pro Ala Thr Pro Glu Glu Ala 260 265 270

Ala Arg Ala Val Leu Gly Val Ala Ser Pro Trp Phe Ser Tyr Val Asn 275 280 285

Gly Glu Thr He Arg Val Thr Gly Gly Arg Asn Met 290 295 300 Xin <210> 30 <211> 327

<212> PRT <213> Penicillium chrysogenum <400> 30

Met Ser Ala Pro Gly Ala Gly His Glu Phe Pro Ala Gin Glu Val Ser 15 10 15

Trp Gin Lys Arg Asp Val Leu Leu Phe Ala Asn Ser lie Gly Val Lys 20 25 30

Ala Asp Glu Leu His Phe Leu Tyr Glu Leu His Pro Asn Phe Ala Val 35 40 45

Phe Pro Thr Tyr Ser Leu lie Leu Arg Met Ser Lys Asn Thr Ser Asp 50 55 60

Phe Arg Thr lie Ala Leu Thr Pro Lys Pro Leu Ala Phe Lys Leu Thr 65 70 75 80

Asp Gin Glu Val Thr Asp Phe Tyr Ala Arg Gin Lys Ala Val His lie 85 90 95

Pro Gly Val Pro Asp Leu Asp His Arg His Gly Val Asp Gly Gin Arg 100 105 110

Lys lie Thr lie Leu Lys Pro Leu Pro Thr Thr Ser Ala Gly Arg Lys 115 120 125

Phe Glu Leu Arg Asn Lys Val lie Gly Val Tyr Asp Lys Gly Lys Pro 130 135 140

Gly Thr Val lie Glu Thr Glu Gin Ser lie Val Asp Lys Glu Ser Gly 145 150 155 160

Glu Val Tyr Ser Lys Val Val Ser Ser Gly Phe Leu Val Gly Gin Gly 165 170 175 43

Gly Trp Gly Gly Pro Lys Gly Pro Ser Thr Val Asn Tyr Ala Pro Pro 180 185 190

Glu Gly Arg Ala Pro Asp Ala Thr His Val Val Gin Ser Asn Ser Glu 195 200 205

Thr Ala His Leu Tyr Arg Leu Asn Gly Asp Tyr Asn Pro Leu His Ala 210 215 220

Thr Pro Glu Pro Gly Gin Lys Met Gly Phe Gly Gly lie lie lie His 225 230 235 240

Gly Leu Phe Ser Trp Asn Ser Ala Ala His Gly lie Leu Arg Glu Phe 245 250 255

Gly Gly Ser Asn Pro Ala Asn Met Lys Glu Phe Gin Ala Arg Phe Ala 260 265 270

Ser Pro Val Arg Pro Gly Asp Lys Leu Thr Thr Glu lie Trp Arg Met 275 280 285

Gly Asn lie Gin Asp Gly Tyr Glu Glu lie Arg Phe Val Thr Lys Asn 290 295 300

Asp Lys Gly Arg Val Val Leu Ser Asn Gly Arg Cys Leu Leu Lys Val 305 310 315 320

Thr Gly Val Lys Ser Lys Leu 325 <210> 31 <211> 901

<212> PRT <213> Penicillium chrysogenum <400> 31

Met Ser Glu Leu Arg Phe Asp Asn Gin Thr Val Val Val Thr Gly Ala 15 10 15

Gly Gly Gly Leu Gly Lys Ala Tyr Ala Leu Phe Phe Ala Ser Arg Gly 20 25 30

Ala Asn Val Val Val Asn Asp Leu Gly Ala Ser His Lys Gly Glu Gly 35 40 45

Lys Ser Gly Lys Ala Ala Asp Val Val Val Glu Glu lie Arg Ala Ala 50 55 60

Gly Gly Lys Ala Val Ala Asn Tyr Asp Ser Val Glu Asn Gly Asp Ala 65 70 75 80 lie lie Glu Thr Ala lie Lys Ala Phe Gly Arg lie Asp lie Leu Leu 85 90 95

Asn Asn Ala Gly lie Leu Arg Asp 100 lie Ser Phe Lys Asn Met 105 110

Lys Asp 44

Ala Asp Trp Asp Leu lie Asn Arg Val His Thr Tyr Gly Ala Tyr Lys 115 120 125

Cys Ala Arg Ala Ala Trp Pro His Phe Arg Lys Gin Lys Phe Gly Arg 130 135 140

Val lie Asn Thr Ala Ser Ala Ala Gly Leu Phe Gly Ser Phe Gly Gin 145 150 155 160 Ala Asn Tyr Ser Ala Ala Lys Leu Gly Gin Val Gly Phe Thr Glu Thr 165 170 175

Leu Ala Lys Glu Gly Ala Lys Tyr Asn lie lie Ala Asn Val lie Ala 180 185 190

Pro lie Ala Ala Ser Arg Met Thr Ala Thr Val Met Pro Pro Asp Val 195 200 205

Leu Glu Asn Leu Lys Pro Asp Trp Val Val Pro Leu Val Ala Ala Leu 210 215 220

Val His Ser Ser Asn Thr Thr Glu Thr Gly Gly lie Tyr Glu Val Gly 225 230 235 240

Gly Gly His Val Ala Lys Leu Arg Trp Glu Arg Ala Lys Gly Ala Leu 245 250 255

Leu Lys Thr Asp Asp Ser Leu Thr Pro Gly Ala lie Ala Arg Lys Trp 260 265 270

Asn Asp Val Asn Asp Phe Ser Gin Pro Glu Tyr Pro Thr Gly Pro Ala 275 280 285

Asp Phe Met Ala Phe Leu Glu Asp Gly Leu Lys Thr Pro Ser Ala Gin 290 295 300

Pro Gly Gin Glu Pro Asp Phe Lys Gly Arg Val Ala Leu Val Thr Gly 305 310 315 320

Gly Gly Ala Gly Leu Gly Arg Ala Tyr Cys Leu Gin Phe Ala Lys Leu 325 330 335

Gly Ala Ser Val Val Val Asn Asp Leu Val Asp Pro Glu Pro Val Val 340 345 350

Gin Glu lie Lys Lys Leu Gly Gly Lys Ala Val Gly Asn Lys Ala Ser 355 360 365

Cys Glu Asp Gly Asp Ala Val Val Lys Ser Ala lie Asp Ala Phe Gly 370 375 380

Arg lie Asp lie Leu Val Asn Asn Ala Gly lie Leu Arg Asp Lys Ala 385 390 395 400

Phe Thr Asn Met Asp Asp Asn Leu Trp Asn Ser Val Val Asn Val His 405 410 415 45

Leu Arg Gly Thr Tyr Lys Val Thr Lys Ala Ala Trp Pro Tyr Phe Leu 420 425 430 Lys Gin Lys Tyr Gly Arg Val Val Asn Thr Ala Ser Thr Ser Gly lie 435 440 445

Tyr Gly Asn Phe Gly Gin Ala Asn Tyr Ala Ala Ala Lys Leu Gly lie 450 455 460 Leu Gly 465 Leu Ser Arg Thr Leu Ala Leu Glu Gly Ala Lys Tyr Asn lie 470 475 480

Lys Val Asn Thr lie Ala Pro Asn Ala Gly Thr Asn Met Thr Arg Thr lie Met 485 490 495 Pro Glu Glu Met Val Gin Ala Phe Lys Pro Asp Tyr Val Ala 500 505 510 Pro Leu Val Val Leu Leu Cys Ser Asp Met Ala Pro Glu Pro Ser Thr 515 520 525 Lys Gly 530 Leu Phe Glu Cys Gly Ser Gly Trp Phe Gly Arg Thr Arg Trp 535 540 Gin Arg 545 Thr Gly Gly His Gly Phe Pro Val Asp Val Lys Leu Thr Pro 550 555 560 Glu Glu Val Val Arg Asn Trp Lys Gin lie lie Asn Phe Asp Asp Gly 565 570 575 Arg Ala Asp His Pro Glu Asp Gly Gin Ala Gly Ala Glu Lys lie Met 580 585 590 Ala Asn Met Ser Asn Arg Val His Gly Asp Thr Ser Thr Glu Asn Glu 595 600 605 Thr Leu 610 Lys Asn lie Lys Lys Ala Lys Ala Leu Ser Ser Glu Gly Thr 615 620 Pro Phe 625 Asn Tyr Glu Asp Arg Asp Val lie Leu Tyr Asn Leu Ser Leu 630 635 640 Gly Ala Lys Arg Thr Asp Leu Pro Leu Val Tyr Glu Asn Asn Asp Gin 645 650 655 Phe Gin Ala Leu Pro Ser Tyr Gly Val Val Pro Trp Phe Asn Thr Ala 660 665 670 Thr Pro Trp Asn Met Asp Asp Leu Val Lys Asp Phe Ser Pro Met Met 675 6 80 685

Leu Leu His Gly Glu Gin Tyr Met Glu Val Arg Lys Phe Pro lie Pro 690 695 700 Thr Thr 705 Ala Asn Thr Leu Thr Tyr Pro Lys Leu lie Asp Val lie Asp 710 715 720 46

Lys Gly Asn Ala Ala lie Val Val 725

Ala Gly Tyr Thr Thr Lys Asp Ala 730 735

Lys Thr Gly Glu Asp Leu Phe Tyr 740

Asn Glu Ser Ser Val Phe lie Arg 745 750

Gly Ser Gly Gly Phe Gly Gly Ser 755 760

Pro Lys Pro Thr Ala Val Arg Pro 765

Lys Ala Ala Thr Ala Ala Tyr Lys 770 775

Ala Pro Gin Arg Gin Pro Asp Ala 780

Val Val Glu Glu Lys Thr Ser Glu 785 790

Asp Gin Ala Ala Leu Tyr Arg Leu 795 800

Asn Gly Asp Arg Asn Pro Leu His 805 lie Asp Pro Glu Phe Ser Lys Val 810 815

Gly Gly Phe Lys Thr Pro lie Leu 820

His Gly Leu Cys Ser Leu Gly Val 825 830

Ser Ala Lys Ala Val Phe Ser Lys Tyr Gly Pro Tyr Lys Asn Leu Lys 835 840 845

Val Arg Phe Ala Gly Val Val Leu Pro Gly Gin Thr Leu Lys Thr Glu 850 855 860

Met Trp Lys Glu Gly Asn Thr Val Leu Phe Gin Ala Thr Val Val Glu 865 870 875 880

Thr Gly Lys Pro Ala lie Thr Gly Ala Gly Ala Glu Leu Leu Glu Gly 885 890 895

Ala Lys Ala Lys Leu 900 <210> 32 <211> 293

<212> PRT <213> Penicillium chrysogenum <400> 32

Met Phe Ala Arg Gin Cys Thr Arg Leu Ala Thr Ser Arg Thr Ser Ala 15 10 15

Pro Leu Thr Ser Tyr Leu Ala Arg Val Arg Gly Tyr Ser Ser Ala Ala 20 25 30

Gly Ser Tyr Glu His lie Leu Thr Ser Thr Pro Lys Pro Gly Val Gly 35 40 45

Leu lie Thr Leu Asn Arg Pro Lys Ala Leu Asn Ala Leu Cys Ser Pro 50 55 60

Leu Phe Lys Glu Leu Asn Glu Ala Leu Ser Asn Tyr Asp Asn Asp Lys 65 70 75 80 47

Ser lie Gly Ala lie lie lie Thr Gly Ser Glu Lys Ala Phe Ala Ala 85 90 95

Gly Ala Asp lie Lys Glu Met Ala Pro Leu Ser Phe Ser Ala Ala Tyr 100 105 110

Ser Asp Asn Phe lie Ala Pro Trp Ser His Leu Ala Thr Ser lie Arg 115 120 125

Thr Pro Val lie Ala Ala Val Ser Gly Tyr Ala Leu Gly Gly Gly Cys 130 135 140

Glu Leu Ala Leu Met Cys Asp lie Leu Tyr Cys Ser Glu Asn Ala Thr 145 150 155 160

Phe Gly Gin Pro Glu lie Lys Leu Gly Thr lie Pro Gly Ala Gly Gly 165 170 175

Ser Gin Arg Leu Thr Arg Ala lie Gly Lys Ser Lys Ala Met Glu Leu 180 185 190 lie Leu Thr Gly Lys Asn Phe Ser Gly Lys Glu Ala Gly Glu Trp Gly 195 200 205

Val Ala Ala Lys Val Val Pro Gly Gly Lys Glu Glu Leu Leu Glu Gin 210 215 220

Ala Tyr Lys Thr Ala Glu Thr lie Ala Ser Tyr Ser Arg Val Ala Val 225 230 235 240

Val Ala Gly Lys Glu Val Val Asn Lys Ser Gin Glu Leu Ser Leu Lys 245 250 255

Glu Gly Val Glu Tyr Glu Arg Arg Leu Phe His Ala Leu Phe Gly Ser 260 265 270

Lys Asp Gin Lys lie Gly Met Thr Ala Phe Ala Glu Lys Lys Lys Pro 275 280 285

Glu Trp Ser His Glu 290

<210> 33 <211> 261 <212> PRT <213> Acinetobacter sp. <400> 33

Met 1 lie Pro Asp Gin 5 Asp Asn Phe Val Glu 10 lie Asp Phe Ser lie 15 Glu Gin lie Ala lie 20 Val Lys lie Asn Arg 25 Pro Ala Ser Lys Asn 30 Ala Leu Asn Thr Glu 35 Val Arg Lys Gin Leu 40 Ala Gin Ala Phe Thr 45 Glu Leu Ser 48

Phe Asn Asp Gin lie Asn Ala lie Val Leu Thr Gly Gly Glu Asp 50 55 60

Phe Ala Ala Gly Ala Asp Leu Lys Glu Met Ala Thr Ala Ser Ser 65 70 75

Asp Met Leu Leu Arg His Thr Glu Arg Tyr Trp Asn Ala lie Ala 85 90 95

Cys Pro Lys Pro Val lie Ala Ala Val Asn Gly Tyr Ala Leu Gly 100 105 110

Gly Cys Glu Leu Ala Met His Thr Asp lie lie lie Ala Gly Lys 115 120 125

Ala Thr Phe Gly Gin Pro Glu lie Lys Val Gly Leu Met Pro Gly 130 135 140

Gly Gly Thr Gin Arg Leu Phe Arg Ala Val Gly Lys Phe His Ala 145 150 155

Arg Met lie Met Thr Gly Val Met Val Pro Ala Glu Glu Ala Tyr 165 170 175 lie Gly Leu Val Ser Gin Val Thr Glu Asp Ser Gin Thr lie Pro 180 185 190

Ala lie Lys Met Ala Gin Ser Leu Ala Lys Met Pro Pro lie Ala 195 200 205

Gin Gin lie Lys Glu Val Ala Leu Met Ser Glu Asp Val Pro Leu 210 215 220

Ala Gly Leu Thr Leu Glu Arg Lys Ser Phe Gin Leu Leu Phe Ser 225 230 235

Glu Asp Lys Asn Glu Gly lie Asn Ala Phe lie Glu Lys Arg Lys 245 250 255

Val Thr 80 Gin Gly Ser Ala Met 160 Leu Thr Leu Asn Thr 240 Pro

Ser Tyr His Gly Lys 260 <210> 34 <211> 136 <212> PRT <213> Aeromonas salmonicida <400> 34 Met Asn Thr Leu Pro Leu Glu lie Gly Gin Lys Ala Ser Leu Thr 15 10 15

Arg Phe Gly Ala Ala Glu Val Glu Ala Phe Ala Gly Leu Ser Glu 20 25 30

Phe Asn Pro Leu His Leu Asp Ser Ala 35 40

Phe Ala Ala Thr Thr Pro 45

Lys Asp Phe 49

Glu Arg Pro lie Val His Gly Met 50 55 Leu Leu Ala Ser Leu Phe Ser Gly 60 Leu Leu Gly Gin Gin Leu Pro Gly 65 70 Lys Gly Thr Val Tyr Leu Gly Gin 75 80 Ser Leu Ser Phe Lys Gin Pro Val 85 Phe Val Gly Asp Glu Val Thr Ala 90 95 Glu Val Glu lie lie Ala Met Arg 100 Ser Asp Lys Pro lie lie Thr Leu 105 110 Ala Thr Arg lie Leu Ala Ala Gly 115 120 Gly Glu Ala Val Val Lys Val Gly 130 135 Gly Ala Leu Ala Leu Ala Val Thr 125 <210> 35 <211> 259 <212> PRT <213> Clostridium kluyveri <400> 35 Met Glu Phe Lys Asn lie lie Leu 1 5 Glu Lys Asp Gly Asn Val Ala Ser 10 15 lie Thr Leu Asn Arg Pro Lys Ala 20 Leu Asn Ala Leu Asn Ala Ala Thr 25 30 Leu Lys Glu lie Asp Ala Ala lie 35 40 Asn Asp lie Ala Glu Asp Asp Asn 45 Val Tyr Ala Val lie lie Thr Gly 50 55 Ser Gly Lys Ala Phe Val Ala Gly 60 Ala Asp lie Ala Glu Met Lys Asp 65 70 Leu Thr Ala Val Glu Gly Arg Lys 75 80 Phe Ser Val Leu Gly Asn Lys lie 85 Phe Arg Lys Leu Glu Asn Leu Glu 90 95 Lys Pro Val lie Ala Ala lie Asn 100 Gl y Phe Ala Leu Gly Gly Gly Cys 105 110 Glu Leu Ser Leu Ser Cys Asp lie 115 120 Arg lie Ala Ser Ser Lys Ala Lys 125 Phe Gly Gin Pro Glu Val Gly Leu 130 135 Gly lie Thr Pro Gly Phe Gly Gly 140 Thr Gin Arg Leu Ala Arg Ala lie 145 150 Gly Val Gly Met Ala Lys Glu Leu 155 160 lie Tyr Thr Gly Lys Val lie Asn 165 Ala Glu Glu Ala Leu Arg lie Gly 170 175 50 201033370

Leu Val Asn Lys Val Val Glu Pro 180

Asp Lys Leu Leu Glu Glu Ala Lys 185 190

Ala Leu Val Asp Ala lie lie Val 195 200

Asn Ala Pro lie Ala Val Arg Met 205

Cys Lys Ala Ala lie Asn Gin Gly 210 215

Leu Gin Cys Asp lie Asp Thr Gly 220

Val Ala Tyr Glu Ala Glu Val Phe 225 230

Gly Glu Cys Phe Ala Thr Glu Asp 235 240

Arg Val Glu Gly Met Thr Ala Phe 245

Val Glu Lys Arg Asp Lys Ala Phe 250 255

Lys Asn Lys gins <210> 36 <211> 257

<212> PRT <213> Clostridium kluyveri <400> 36

Glu Lys Gin Asn Gly lie Thr lie 10 15

Met Thr Tyr Lys Thr Leu Leu Leu 1 5 lie Lys Met Asn Thr Pro His Asn 20

Leu Asn Ala lie Ser Gin Gin Ser 25 30

Val Glu Asp Leu Phe Ala Val Leu 35 40

Gin Val lie Lys Asn Asp Asp Asn 45

Cys Arg Val Val He Leu Thr Gly 50 55

Glu Gly Lys Gly Phe lie Gly Gly 60

Ala Asp lie Lys His Met Ala Cys 65 70

Leu Asp Ala lie Glu Gly Gly Gin 75 80

Phe Cys Phe Ala Val Ser Lys Cys 85

Thr Leu Glu Met Glu Lys Met Gly 90 95

Lys Val Phe lie Ala Ala Val Asn 100

Gly Phe Ala Leu Gly Ala Gly Leu 105 110

Glu Val Ala Leu Gly Cys Asp lie 115 120

Arg lie Phe Ser Lys His Ala Lys 125 lie Gly Phe Pro Glu Thr Gly Leu 130 135

Gly Val lie Pro Gly Ala Gly Gly 140

Ala Gin Arg Leu Gin Arg Leu Val 145 150

Gly lie Gly Lys Ala Ser Glu lie 155 160 lie Phe Thr Gly Asp lie lie Gly 165

Ala Asp Asp Ala Leu Arg Phe Gly 170 175 51 lie Ala Asn Gin Val 180

Thr Glu Pro Glu Ser Leu Met Asp Thr Ala Met 185 190

Ser Met Ala Glu Lys lie Leu Thr Lys Ser Pro Val Gly Thr Arg Leu 195 200 205

Ala Lys Glu Ala Leu Gin Lys Gly Arg Asp Thr Asp Leu Glu Lys Ala 210 215 220

Leu Glu Tyr Asp Lys Asn Leu Phe Gly Leu Cys Phe Ser Thr Glu Asp 225 230 235 240

Lys Lys Glu Gly Met Ala Ala Phe lie Glu Lys Arg Lys Pro Val Phe 245 250 255

Lys

<210> 37 <211> 255 <212> PRT <213> Escherichia coli <400> 37

Met Ser Glu Leu lie Val Ser Arg Gin Gin Arg Val Leu Leu Leu Thr 15 10 15

Leu Asn Arg Pro Ala Ala Arg Asn Ala Leu Asn Asn Ala Leu Leu Met 20 25 30

Gin Leu Val Asn Glu Leu Glu Ala Ala Ala Thr Asp Thr Ser lie Ser 35 40 45

Val Cys Val lie Thr Gly Asn Ala Arg Phe Phe Ala Ala Gly Ala Asp 50 55 60

Leu Asn Glu Met Ala Glu Lys Asp 65 70

Leu Ala Ala Thr Leu Asn Asp Thr 75 80

Arg Pro Gin Leu Trp Ala Arg Leu 85

Gin Ala Phe Asn Lys Pro Leu lie 90 95

Ala Ala Val Asn Gly Tyr Ala Leu 100

Gly Ala Gly Cys Glu Leu Ala Leu 105 110

Leu Cys Asp Val Val Val Ala Gly Glu Asn Ala Arg Phe Gly Leu Pro 115 120 125

Glu lie Thr Leu Gly lie Met Pro Gly Ala Gly Gly Thr Gin Arg Leu 130 135 140 lie Arg Ser Val Gly Lys Ser Leu Ala Ser Lys Met Val Leu Ser Gly 145 150 155 160

Glu Ser lie Thr Ala Gin Gin Ala Gin Gin Ala Gly Leu Val Ser Asp 165 170 175 52

Val Phe Pro Ser Asp Leu Thr Leu Glu Tyr Ala Leu Gin Leu Ala Ser 180 185 190

Lys Met Ala Arg His Ser Pro Leu Ala Leu Gin Ala Ala Lys Gin Ala 195 200 205

Leu Arg Gin Ser Gin Glu Val Ala Leu Gin Ala Gly Leu Ala Gin Glu 210 215 220

Arg Gin Leu Phe Thr Leu Leu Ala Ala Thr Glu Asp Arg His Glu Gly 225 230 235 240 lie Ser Ala Phe Leu Gin Lys Arg Thr Pro Asp Phe Lys Gly Arg 245 250 255 <210> 38 <211>

<212> PRT <213 > Pseudomonas putida <400> 38

Met Pro Arg Tyr Leu Asp Val Gin Ala Pro Glu Asn Gly Val Gin Leu 1 5 10 15 lie Thr Leu Gin Arg Pro Glu Ala Leu Asn Ala Leu Cys Thr Glu Leu 20 25 30

Leu Ala Glu Leu Ala Thr Ala Leu Asp Ala Ala Ala Arg Asp Asp Gin 35 40 45 lie Gly Val Val Val Leu Thr Gly Ser Arg Lys Ala Phe Ala Ala Gly 50 55 60

Ala Asp worker Arg Glu Met Ala Glu Arg Asp Leu Val Gly lie Leu Asn 65 70 75 80

Asp Pro Arg Val Ala His Trp Gin Arg lie Ala Ala Phe Ala Lys Pro 85 90 95

Leu lie Ala Ala Val Asn Gly Tyr Ala Leu Gly Gly Gly Cys Glu Leu 100 105 110

Val Met Cys Ala Asp lie Val lie Ala Gly Ser Asp Ala Arg Phe Gly 115 120 125

Gin Pro Glu lie Asn Leu Gly lie lie Pro Gly Ala Gly Gly Thr Gin 130 135 140

Arg Leu Leu Arg Ala Val Gly Lys Pro Leu Ala Met Gin Met Val Leu 145 150 155 160

Thr Gly Glu Ala lie Thr Ala Arg His Ala Gin Gin Ala Gly Leu Val 165 170 175

Ser Glu lie Thr Gin Pro Glu Phe Thr Val Glu Arg Ala Met Gin lie 180 185 190 53

Ala Arg Asn lie Ala Ala Lys Ala 195 200

Pro Leu Ala Val Arg Leu Ala Lys 205

Glu Ala Leu Leu Lys Ala Gly Asp 210 215

Thr Asp Leu Ala Ser Gly Leu Arg 220

Phe Glu Arg His Ala Phe Thr Leu 225 230

Leu Ala Gly Thr Ala Asp Arg Asp 235 240

Glu Gly lie Gin Ala Phe Gin Glu 245

Lys Arg Pro Ala Arg Phe Gin Gly 250 255

Arg

<210> 39 <211> 263 <212> PRT <213> Pseudomonas putida <400> 39

Ser lie Glu Asp Gly Val Ala Phe 10 15

Met Thr Phe Gin His lie Leu Phe 1 5

Leu Ser Leu Asn Arg Pro Glu Gin 20

Leu Asn Ser Phe Asn Ala Ala Met 25 30

His Leu Glu Val Arg Glu Ala Leu 35 40

Lys Gin Val Arg Gin Ser Ser Asp 45

Ala Arg Val Leu Leu Leu Thr Ala 50 55

Glu Gly Arg Gly Phe Cys Ala Gly 60

Gin Asp Leu Ser Asp Arg Asn Val 65 70

Ala Pro Asp Ala Glu Val Pro Asp 75 80

Leu Gly Glu Ser lie Asp Lys Phe 85

Tyr Asn Pro Leu Val Arg Thr Leu 90 95

Arg Asp Leu Pro Leu Pro Val lie 100

Cys Ala Val Asn Gly Val Ala Ala 105 110

Gly Ala Gly Ala Asn lie Pro Leu 115 120

Ala Cys Asp Leu Val Leu Ala Gly 125

Arg Ser Ala Ser Phe lie Gin Ala 130 135

Phe Cys Lys lie Gly Leu Val Pro 140

Asp Ser Gly Gly Thr Trp Leu Leu 145 150

Pro Arg Leu Val Gly Met Ala Arg 155 160

Ala Lys Ala Leu Ala Met Leu Gly 165

Glu Arg Leu Gly Ala Glu Gin Ala 170 175

Gin Gin Trp Gly Leu lie His Arg 180

Val Val Asp Asp Ala Ala Leu Arg 185 190 54

Asp Glu Ala Leu Thr Leu Ala Arg Gin Leu Ala Ser Gin Pro Thr Tyr 195 200 205

Gly Leu Ala Leu lie Lys Arg Ser Leu Asn Ala Ser Phe Asp Asn Gly 210 215 220

Phe Asp Glu Gin Leu Glu Leu Glu Arg Asp Leu Gin Arg Leu Ala Gly 225 230 235 240

Arg Ser Glu Asp Tyr Arg Glu Gly Val Ser Ala Phe Met Asn Lys Arg 245 250 255

Thr Pro Ala Phe Lys Gly Arg 260 <210> 40

<211> 699 <212> PRT <213> Rhodopseudomonas palustris <400> 40

Met Ser Glu Val Val Thr Arg Ala Thr Gin Asp Gin Val Ala He Val 15 10 15

Thr Val Asp Ser Pro Pro Val Asn Ala Leu Ser Ala Ala Val Arg Arg 20 25 30

Gly lie Leu Glu Asn Val Asn Ala Ala Val Ala Asp Pro Ala Val Gin 35 40 45

Ala lie Val Leu Val Cys Ala Gly Arg Thr Phe lie Ala Gly Ala Asp 50 55 60 lie Thr Glu Phe Gly Lys Pro Pro Gin Pro Pro Ala Leu. Asn Asp Val 65 70 75 80 lie Ala Ala Leu Glu Asn Ser Pro Lys Pro Thr lie Ala Ala lie His 85 90 95

Gly Thr Ala Leu Gly Gly Gly Leu Glu Val Ala Leu Gly Cys His Phe 100 105 110

Arg Val Ala Val Lys Glu Ala Lys Leu Gly Leu Pro Glu Val Lys Leu 115 120 125

Gly Leu Leu Pro Gly Ala Gly Gly Thr Gin Arg Leu Pro Arg Ala Val 130 135 140

Gly Pro Glu Leu Ala Val Gin Met lie Val Gly Gly Ser Pro lie Gly 145 150 155 160

Ala Ala Glu Ala Leu Lys His Gly Leu Val Glu Glu Val Val Glu Asn 165 170 175

Leu Val Ala Gly Ala Val Ala Phe Ala Lys Lys Val Leu Ala Glu Lys 180 185 190 55

Arg Pro Leu Arg Arg Leu Arg Asp Asp Asp Ser Lys Leu Ala Ala Ala 195 200 205 Lys Ala 210 Asp Arg Ser lie Phe Thr Asn Ala Val Ala Ala Met Thr Lys 215 220 Lys Ala 225 Arg Gly Leu Glu Ala Pro Phe Ala Cys Ala Asp Ala lie Gly 230 235 240 Ala Ala lie Asp Leu Pro Phe Glu Glu Gly Leu Lys Lys Glu Arg Glu 245 250 255 Gly Phe Met Lys Leu Val Val Ser Asp Gin Ser Lys Ala Gin Arg Tyr 260 265 270 Ala Phe Phe Ala Glu Arg Glu Ala Ala Lys Val Asp Gly Val Pro Asp 275 280 285 Gly Thr 290 Lys Pro Arg Pro Val Ser Arg Val Ala lie lie Gly Ala Gly 295 300 Thr Met 305 Gly Gly Gly lie Ala Met Ser Phe Ala Asn Ala Gly lie Pro 310 315 320 Val Thr Leu lie Glu Thr Gly Glu Glu Gin Leu Lys Arg Gly Leu Gly 325 330 335 lie Met Gin Lys Asn Trp Glu Ala Thr Ala Ala Arg Gly Gly Leu Pro 340 345 350 Pro Asp Ala Pro Ala Lys Arg Met Ala Leu lie Thr Gly Leu Val Gly 355 360 365 Leu Glu 370 Asn Val Lys Asp Ala Asp Leu lie lie Glu Ala Val Phe Glu 375 380 Thr Met 385 Ala Val Lys Lys Glu Val Phe Thr Ala Val Asp Ala His Ala 390 395 400 Lys Pro Gly Ala Val Leu Ala Ser Asn Thr Ser Tyr Leu Ser lie Asp 405 410 415 Glu lie Ala Ala Thr Thr Lys Arg Pro Gin Asp Val Leu Gly Met His 420 425 430 Phe Phe Ser Pro Ala Asn Val Met Lys Leu Cys Glu lie Val Arg Gly 435 440 445 Ala Lys 450 Thr Ala Pro Asp Ala Leu Leu Thr Ala Val Ser lie Ala Lys 455 460 Lys lie 465 Ala Lys Val Pro Val Val Val Gly Val Cys Asp Gly Phe Val 470 475 480

Gly Asn Arg Met Leu Ala Ala Arg Ser Lys Gin Ser Glu Lys Leu Leu 56 201033370 485 490 495

Phe Glu Gly Ala Leu Pro Gin Gin Val Asp Ala Val Val Thr Lys Phe 500 505 510

Gly Met Pro Met Gly Pro Phe Ala Met Gly Asp Leu Ala Gly Leu Asp 515 520 525 lie Gly Trp Arg Ser Arg Lys Asp Arg Gly He Lys Ser Glu lie Ala 530 535 540

Asp Ala Leu Cys Glu Ala Gly Arg Phe Gly Gin Lys Thr Gly Lys Gly 545 550 555 560

Tyr Tyr Lys Tyr Glu Gin Gly Ser Arg Ala Pro Met Pro Asp Pro Glu 565 570 575

Val Glu Thr Leu lie Asn Asp Thr Leu Ala 580 585

Arg Asp lie Thr Asp Glu Glu lie Leu Glu 595 600 lie Asn Glu Gly Ala Arg lie Leu Glu Glu 610 615

Lys Leu Gly Leu Lys Arg 590 Arg Met Val Tyr Pro Met 605 Lys lie Ala Ala Arg Pro 620

Ser Asp lie Asp Val Val Trp Leu Tyr Gly Tyr Gly Trp Pro lie Tyr 625 630 635 640

Arg Gly Gly Pro Met His Tyr Ala Asp Ser Val Gly Leu Lys His lie 645 650 655

Ala Glu Arg Leu Ser Ala Tyr Ala Lys Ala Thr Asn Asp Pro Ser Leu 660 665 670

Glu Pro Ala Pro Leu Leu Ala Arg Leu Ala Ala Glu Gly Lys Thr Phe 675 680 685

Ala Ser Leu Thr Gin Pro Ser Lys Ala Ala Ala 690 695 <210> 41 <211> 242 <212> PRT <213> Rhodococcus sp <400>

Met Asn Thr Leu Lys lie Glu Asp Arg Asp Asp Arg Met Val Val Thr 15 10 15

Leu His Arg Pro Ala 20

Gin Arg Asn Ala lie Asn Ala Glu Met lie Gly 25 30

Glu Leu His Gin Val Cys Ala Ser Leu Glu Ala Thr Pro Lys Pro Met 35 40 45

Leu Leu Thr Gly Glu Gly Asp His Phe Ala Gly Gly Ala Asp lie Ala 57 50 55 60

Glu Leu Arg Glu Arg Gly Arg Asp Glu Ala Phe Ala Gly lie Asn Arg 65 70 75 80

Asn Leu Phe Asp Arg lie Ala Lys Leu Pro Met Pro Thr Val Ala Ala 85 90 95

Val Ser Gly Tyr Ala Leu Gly Gly Gly Ala Glu Leu Ser Tyr Ala Cys 100 105 110

Asp lie Arg lie Ala Thr Glu Thr Ala Val Phe Gly Asn Pro Glu Pro 115 120 125

Gly Leu Gly lie Met Ala Ala Ala Gly Ala Ser Tyr Arg Leu Pro Glu 130 135 140

Leu Val Gly Thr Ser Val Ala Lys Gin Val Leu Leu Gly Gly Arg Asn 145 150 155 160

Leu Asp Ala Gin Asp Ala Leu Arg Ser Gly Leu Val Met Ser Val Val 165 170 175

Ala Pro Gly Glu His lie Asp Ala Ala His Lys Val lie Asp Arg lie 180 185 190

Thr Arg Ser Ala Pro Leu Ala Leu Lys Leu Thr Lys Met lie Val Asp 195 200 205

Ala Pro Gly Ser His Pro Phe Ala Asp Asp lie Ala Gin Ala Val Leu 210 215 220

Phe Glu Ser Arg Asp Lys His Asp Arg Met Thr Ala Phe Leu Glu Lys 225 230 235 240

Lys Lys

<210> 42 <211> 689 <212> PRT <213> Penicillium chrysogenum <400>

Met Pro Asp Phe Thr Asp Asn Leu Arg Pro Ser Gin Pro Asp Gly Pro 15 10 15

Thr Thr Leu Ala Arg Glu Arg Gin Lys Ser Asn lie Ala Thr Glu Glu 20 25 30

Leu Gly Gin His Leu Leu Gly Ser Asp Gly Phe Leu Val Arg Gin Ala 35 40 45

Arg lie Leu Pro lie lie Gin Gin Glu Pro Leu Phe Ser Lys Asp Gin 50 55 60

Gin Gin Asn Leu Ser Arg Pro Glu Arg Phe Lys Leu Gly Val Ala Arg 58 65 70 75 80

Ala Lys Leu Leu Arg Gin Met Lys Asp Thr His Lys Trp Ser His Leu 85 90 95

Glu Tyr Gin Met Ala Glu Tyr Leu Val Asp Asp Val Ser Pro Tyr Phe 100 105 1X0

Leu His Met Glu Met Phe lie Thr Thr lie Arg Glu Gin Ala Ser Glu 115 120 125

Glu Gin Gin Ala His Trp Leu Pro Leu lie Glu Leu Trp Lys lie lie 130 135 140

Gly Ala Tyr Ala Gin Thr Glu Leu Gly His Gly Ser Asn Val Arg Gly 145 150 155 160

Leu Glu Leu Glu Ala Arg Trp Asp Asn Arg Thr Lys Glu Phe Val Leu 165 170 175

His Ser Pro Thr Leu Thr Ala Ser Lys Trp Trp Asn Gly Ser Leu Gly 180 185 190

Arg Leu Ala Asn His Ala lie Val Val Ala Gin Leu Leu Leu Pro Asp 195 200 205

Pro Ser Ser Pro Asp Gin Tyr Val Ser His Gly Pro His Pro Phe lie 210 215 220

Val Gin Val Arg Asp Met Lys Thr His Gin Pro Leu Asn Gly lie Val 225 230 235 240

Val Gly Asp lie Gly Pro Lys Tyr Gly Tyr lie Thr Met Asp Asn Ala 245 250 255

Tyr Met Leu Phe Asp Gin Phe Arg lie Pro His Ser Ala Met Leu Ser 260 265 270

Arg Tyr Ser Lys Val Asp Leu Asn Thr Gly lie Tyr Thr Lys Pro Glu 275 280 285

Lys Pro Ala Leu Val Tyr Gly Ser Leu Thr Tyr Val Arg Ser Asn Met 290 295 300

Val His Arg Ala Arg Leu Val Leu Ala Arg Ala Val Thr Val Ala Val 305 310 315 320

Arg Tyr Ser Ser Val Arg Arg Gin Phe Gin Asp Arg Asp Gly Asp Lys 325 330 335

Thr Gly Pro Glu Met Ser Val Leu Asp Tyr Pro Thr Val Gin lie Arg 340 345 350 lie Leu Pro Leu Leu Ala Thr Thr Phe Ala Leu His Tyr Thr Gly Leu 355 360 365 59

Ala Met Gin Thr Val Tyr Lys Asn Ala Arg Gin Asp lie Glu Glu Gly 370 375 380

Asn Phe Asn Ser Leu Ala His Met His Ser Met Ser Ser Gly Leu Lys 385 390 395 400 Ser Leu Cys Thr lie 405 Phe Ala Ala Asp Gly lie Glu Thr Cys Arg Arg 410 415 Ala Met Gly Gly His 420 Gly Phe Gly Gly Gly Ser Gly Leu lie Gin Val 425 430 Asn Asn Asp Tyr Leu 435 Ser Lys Pro Thr Val Glu Gly Asp Asn Trp Met 440 445 He Thr Gin Gin Val 450 Ala Ala Tyr Val lie Lys Lys Met Thr Ala Ala 455 460 Val Gly Ser Pro Asp 465 Thr Pro Gly lie Asp Glu Thr Asp Ala Arg Phe 470 475 480 Lys Glu Phe lie Arg 485 Asn Lys Arg Arg Pro Glu Ser Glu Lys Arg Thr 490 495 Tyr Asp lie Leu Asn 500 Ser Asp Leu Asp lie Val Lys Ser Phe Glu Leu 505 510 Arg Ala Thr Ala Met 515 Ala Ser Ser Thr Lys Leu lie Arg Val lie Lys 520 525 Lys Arg Asn Trp Asn 530 Ser Leu Leu lie Gin Leu His Lys Leu Ser Arg 535 540 Ala Gin Ser Glu Ser 545 lie lie Val Ala Thr Phe Phe Asp Ala Leu Ser 550 555 560 Asn Asp Lys Thr Leu 565 Ser Ala Pro Thr Lys Asn Val Leu Trp Asp Cys 570 575 Tyr Arg Leu Phe Ala 580 Leu Tyr Ser Met Glu Asn Glu Ser Phe Glu P He 585 590 Leu Arg Thr Asn Ala 595 Val Ser Gin Thr Asp Leu Asp Ser Leu Ala Ser 600 605

Arg Val Gin Asp Leu Met Ala Arg lie Arg Pro His Ala Val Thr Leu 610 615 620 Val Asp Ser Trp Met 625 lie Pro Asp Tyr Leu Leu Asp Ser Ala Leu Gly 630 635 640

Arg Tyr Asp Gly Arg Val Tyr Glu Asp Leu Phe Asn Arg Ala His Arg 645 650 655

Leu Asn Pro Leu Asn Arg lie Thr Phe Asn Pro Asn Tyr Trp Glu Asp 660 665 670 60 201033370

Glu lie Val Lys Gly Ser Gly Asp Asn Gly Arg Gly lie Leu Ser Lys 675 680 685

Leu <210> 43 <211> 425 <212> PRT <213> Penicillium chrysogenum <400>

Met Ser Val Ser Asn Val Pro Phe Ala Asp Pro Leu Trp Leu Asn Arg 1 5 10 15

Lys His Ser Pro Tyr Tyr Lys Asp Ser His Arg Lys Leu Gin Lys Glu 20 25 30

Val Arg Gin Tyr Val Asp Glu His lie Ser Pro Phe Cys Glu Glu Trp 35 40 45

Glu Lys Gin Gly Phe Val Pro Pro Glu Ala Gin Lys Arg His Ala Glu 50 55 60

Leu Gly Tyr Thr Ala Val Ala Ser Phe Pro Leu Ala Ala Asp Tyr Leu 65 70 75 80

Asp Gly Gin Arg Leu Pro Gly Asp lie Asn Pro Tyr Glu Trp Asp Gly 85 90 95

Phe His Asp lie Val Val lie Asp Glu Leu Ala Arg Cys Gly Tyr Leu 100 105 110

Gly lie Val Trp Ala Leu Gly Cys Gly Asn Ser lie Gly Gly Pro Pro 115 120 125

Lie lie Asn Phe Gly Asn Glu Glu Gin Lys Arg Arg Phe Leu Pro Asp 130 135 140

Met Leu Lys Gly Lys lie Arg Phe Cys Leu Gly Val Thr Glu Pro Asp 145 150 155 160

Ala Gly Ser Asp Val Ala Gly lie Thr Thr Val Ala Glu Arg Lys Gly 165 170 175

Asp Ala Tyr lie Val Asn Gly Ala Lys Lys Trp lie Thr Asn Gly lie 180 185 190

Phe Ala Asp Phe Cys Thr Ala Ala Val Arg Thr Gly Gly Ser Gly Thr 195 200 205

His Gly lie Ser Ala Leu Val lie Pro Met Lys Ala Pro Gly Val lie 210 215 220

Cys Arg Lys lie Glu Asn Ser Gly Val His Ala Ser Gly Ser Thr Tyr 225 230 235 240 61 lie Glu Phe Asp Gin Val Glu Val Pro Val Asp Asn Leu Leu Gly Glu 245 250 255

Glu Asn Lys Gly Phe Pro Val lie Met Asn Asn Phe Asn 260 265 s o i 7 H 2

Glu Arg

Leu Trp Leu Ala Cys Thr Ser Leu Arg Met Ala Arg Val Cys Ala Glu 275 280 285

Asp Ala Tyr Gin His Ala lie Thr Arg Glu Thr Phe Gly Lys Arg Leu 290 295 300 lie Glu Asn Gin lie lie Arg Ser Lys Phe Ser Ala Met Ala Arg Ser 305 310 315 320

Leu Asp Ser Asn Tyr Ala Trp Met Glu Gin Leu Val Tyr lie Ala Glu 325 330 335 lie Ala Lys Lys Glu Gly Thr Asp Ala Gly Thr Gly Gly Leu Phe Ala 340 345 350

Asn Leu Lys Val Leu Ala Gly Gin Thr Leu Glu Lys Val Asn Arg Glu 355 360 365

Ser Gin Gin Val Met Gly Gly Leu Gly Tyr Ser Lys Asn Gly Arg Gly 370 375 380

Ser Arg lie Glu Gin Val Ser Arg Asp Val Arg Val Met Val Val Gly 385 390 395 400

Gly Gly Ser Glu Glu lie Leu Ser Glu Leu Ala Val Asn Gin Glu lie 405 410 415

Lys Ala Met Lys Lys Gin Ser Lys Leu 420 425

<210> 44 <211> 605 <212> PRT <213> Penicillium chrysogenum <400> 44

Met Ala Ala Gin Ser Arg Val Pro Ala Thr Ala Glu Ser Gly Thr Phe 15 10 15

Thr Gin Leu Gly Pro Leu Pro Asn Thr Tyr Thr Ser Asp lie Ser Leu 20 25 30

Gin Arg Met Leu Gly Trp Tyr Leu Pro Ala Gin Thr Leu Lys Leu lie 35 40 45

Glu Pro His Leu Ala Gin Leu Gly Glu Glu Ala Val Ser Pro Gin Val 50 55 60

Phe Ala Trp Asn Ala Asp Ala Glu Thr Asn Leu Pro Tyr Val Lys Lys 65 70 75 80 62

Tyr Asn Val Trp Gly 85 Gin Arg Tyr Ala Tyr Asp Arg Leu Val Thr Thr 90 95 Asp Gly Trp Lys Gin 100 Leu Gly Lys Trp Gly Ala Lys His Gly Val Val 105 110 Ser Leu Gly Tyr Asp 115 His Thr Tyr Gly Val Tyr Arg Arg Thr Ala Gin 120 125 Tyr Ala Ala Val Tyr 130 Leu Tyr Ala Pro Ser Ser Ala Met Tyr Arg Cys 135 140

Pro Met Ser Met Ser Asp Gly Ala Ala Leu Thr Ser Gly Gin Trp Met 145 150 155 160 Thr Glu Arg Ala Gly 165 Gly Ser Asp Val Gin Asn Thr Glu Thr Trp Ala 170 175 Thr Tyr Ala Pro Leu 180 Pro Gin Glu Ser Lys Thr Ser Asp Val Leu Ala 185 190 Glu Gly Asp Tyr Leu 195 lie Ser Gly Phe Lys Phe Phe Ser Ser Ala Thr 200 205 Asp Ala Asn Val Ala 210 Phe Leu Leu Ala Lys Thr Asp Ser Gly Lys Leu 215 220 Ser Thr Phe lie Ala 225 Pro Leu Arg Lys Thr Ser lie Gly Ala Asp Gly 230 235 240 Lys Pro Glu Glu Thr 245 Ser Asn Gly Val Arg lie His Arg Phe Lys Asn 250 255 Lys Leu Gly Thr Lys 260 Glu Leu Pro Thr Ala Glu Leu Glu Leu Lys Gly 265 270 Met Arg Ala His Leu 275 Val Gly Glu Leu Asp Gin Gly lie Val Thr lie 280 285 Ala Pro Leu Leu Asn 290 Thr Thr Arg lie Gin Thr Leu Leu Gly Thr Leu 295 300 Ser Thr Trp Arg Arg 305 Ala lie Ser lie Thr Lys Asn Phe Ala Lys Ser 310 315 320

Arg Thr Thr Val Gly Glu Pro Leu Trp Leu lie Pro Met His Leu Arg 325 330 335 Leu Leu Ala Asp Val 340 Glu Val Lys His Arg Gly Ala lie Asn Leu Ala 345 350

Phe Phe Thr lie Ala Val Met Gly Leu lie Glu Asn Pro Ser Ser Pro 355 360 365 Ala Arg His Ala His 370 Met Pro Arg Asp Pro Thr Glu Ala Lys Val Val 375 380 63

Phe Arg Val Leu Thr Ala Thr Ser Lys Gly Val Val Ser Lys Met 385 390 395

Met Val Gly Val Gin Glu Cys Gin Glu Ala lie Gly Gly Val Gly 405 410 415 lie Asp Glu Pro Asp Glu Pro Glu Phe Asn lie Ser Arg Leu Leu 420 425 430

Ser Ala Ala Val Tyr Pro lie Trp Glu Gly Thr Thr Asn Val Leu 435 440 445

Ser Glu Leu Val Arg Phe Leu Met Lys Gly Asp Asn Leu Ser lie 450 455 460

Ser Gly Trp Leu Gly His Val Val Ser Leu lie Arg Thr Pro Ser 465 470 475

Ala Gly Ala Leu Lys Gin Ala Met Ala Ser Tyr Leu Ser Arg Val 485 490 495

Ser Thr Arg Pro Gin Ala Ala Leu Leu Ala Asp Ala Arg Arg Val 500 505 510

Phe Thr Phe Ala Trp lie Leu Ser Gly Ala Leu Leu Thr Leu Asp 515 520 525

Glu Arg Asp Glu Asp Glu Val Ala Met Glu lie Ala Arg Arg Trp 530 535 540

Leu Leu Gly Glu Gly Gly Val Gly Glu Phe Val Tyr Arg Asp lie 545 550 555

Lys Pro Tyr Gin Cys Phe Asn Leu Arg Ser Gly Arg Asp Glu His 565 570 575

Arg Leu Asp Cys Lys lie Ala Trp Gly Val Glu Leu Pro Gly Lys 580 585 590

Ser 400 Tyr Arg Ala Leu Leu 480 Thr Met Ala Val Ala 560 Thr He

Val Phe Gly His Arg Ser Leu Ser Glu Ser Ser Lys Leu 595 600 605 <210> 45 <211> 425 <212> PRT <213> Penicillium chrysogenum <400> 45 Met Met Asn Arg Ser Leu Leu Arg Ala Ala Ala Arg Ser lie Gin 15 10 15

Gly Pro Thr Ser Val Ala Gly Arg Arg Tyr Ala Ser Ser Ala Val 20 25 30

Asn Trp Glu Asp Pro Leu Ala Ala Ala Glu Leu Phe Thr Asp Glu 35 40 45

Ala Phe Glu 64

Leu Ala lie Gin Asp Thr Ala Arg Gin Tyr Cys Gin Asp Lys Leu Ala 50 55 60

Pro Arg Val Leu Glu Ala Tyr Arg Asn Glu Asp Tyr Asp Arg Arg lie 65 70 75 80

Leu Glu Glu Met Gly Asp Leu Gly Leu Leu Gly Ala Ser lie Glu Gly 85 90 95

Tyr Gly Cys Ala Gly Val Ser Thr Val Ala Ser Gly Leu He Thr Lys 100 105 110

Glu Val Glu Arg Val Asp Ser Gly Tyr Arg Ser Gly Met Ser Val Gin 115 120 125

Ser Ser Leu Ala Met Thr Gly lie Tyr Glu Phe Gly Thr Glu Glu Gin 130 135 140

Lys Gin Arg Phe Leu Pro Ser Leu Ala Lys Gly Thr Leu Ala Gly Cys 145 150 155 160

Phe Gly Leu Thr Glu Pro Asn His Gly Ser Asp Pro Gly Ser Met Glu 165 170 175

Thr Val Ala Arg Glu His Pro Thr Gin Lys Gly Met Tyr Leu Leu Ser 180 185 190

Gly Ser Lys Thr Trp lie Thr Asn Ser Pro lie Ala Asp Val Ala Leu 195 200 205

Val Trp Ala Lys Leu Glu Ser Thr Gly Lys lie Arg Gly Phe lie Val 210 215 220

Glu Arg Glu Arg Ala Thr Pro Gly Ser Tyr Glu Thr Pro Ala lie Lys 225 230 235 240

Asn Lys Ser Ala Leu Arg Ala Ser lie Thr Gly Met lie Gin Met Asp 245 250 255

Asn Cys Pro Val Pro Ala Glu Asn Met Leu Pro Glu Val Glu Gly Leu 260 265 270

Lys Gly Pro Phe Thr Cys Leu Asn Ser Ala Arg Leu Gly lie Ala Phe 275 280 285

Gly Ala Met Gly Ala Leu Glu Asp Cys Leu Ala Arg Ala Arg Glu Tyr 290 295 300

Ser Leu Glu Arg Lys Gin Phe Lys Gly Asn Pro Leu Ala Lys Tyr Gin 305 310 315 320

Leu lie Gin Met Lys Leu Ala Asn Ala Ala Thr Asp Ala Ala Tyr Gly 325 330 335

Thr Leu Ala Ala Val Gin Val Ala Arg Leu Lys Asp Ala Gly Lys Ala 65 201033370 340 345 350

Thr Pro Glu Met lie Ser Met lie Lys Arg Gin Asn Cys Asp Arg Ala 355 360 365

Leu Ala Asn Ser Arg lie Leu Gin Glu Val Phe Gly Gly Asn Ala Ala 370 375 380

Ser Asp Glu Tyr His lie Ala Arg His Val Ala Asn Leu Phe Val Val 385 390 395 400

Gin Thr Tyr Glu Gly Gin Ser Asp lie His Ala Leu lie Leu Gly Arg 405 410 415

Ala lie Thr Gly Lys Gin Ala Phe Val 420 425

<210> 46 <211> 633

<212> PRT <213> Penicillium chrysogenum <400> 46

Met Ser Ser Ser Gin Pro Ala Thr Ala Asp Ser Gly Tyr lie Thr Asp 15 10 15

Pro Gly Pro Leu Glu Asn Thr Tyr Thr Ser Asp Pro Ser Leu Gin Arg 20 25 30

Ala Leu Ala Trp Tyr Leu Pro Ser Ala Thr Leu Gin Ser Val Gin Pro 35 40 45

His Leu Thr Gin Phe Gly Ala Glu Ala lie Ser Glu Gin Val Arg Glu 50 55 60

Trp Ser Ala Asp Ala Glu Arg Asn Val Pro Tyr Val Lys Ser His Asn 65 70 75 80

Val Trp Gly Lys Arg Tyr Asp Tyr Asp Arg Leu Val Thr Thr Glu Gly 85 90 95

Trp Lys Gin Leu Gly Lys Trp Gly Ala Arg Asn Arg lie Val Ser Ala 100 105 110

Gly Tyr Asp Lys Ser Leu Gly Val Asp Arg Arg Thr Val Gin Tyr Ala 115 120 125

Leu Asn Tyr Leu Tyr Ser Pro Ser Ser Gly Leu Tyr Ser Cys Pro lie 130 135 140

Ser Met Thr Asp Gly Ala Ala Phe lie Leu Ser Ser Arg lie Asn Lys 145 150 155 160

Leu Pro Ser Thr His 165

Pro Phe His Thr Ala Phe Gin Gly Leu lie Ser 170 175

Glu Lys Asp Asp His Trp Thr Ser Gly Gin Trp Met Thr Glu Arg Ala 66 180 185 190

Gly Leu lie 225 Leu Pro Ser Glu lie 305 Val Ala Glu Glu Leu 385 His Thr Gin Asp Asn 465

Gly Ser Asp Val Gin Asn Thr Glu Thr Trp Ala Thr Tyr Ser Pro 195 200 205

Ala Ser Ser Ser Gly Ser Glu Pro Leu Gly Asp Gly Asp Tyr Leu 210 215 220

Ser Gly Phe Lys Phe Phe Ser Ser Ala Thr Asp Ala Asn Leu Ala 230 235 240

Leu Leu Ala Lys Thr Pro Ser Gly Lys Leu Ser Thr Phe Leu Ala 245 250 255

Leu Arg Arg Thr Val Val Gly Gly Asp Gly Val Ser Arg Val Val 260 265 270

Asn Gly Val Arg lie His Arg Leu Lys Asn Lys Leu Gly Thr Lys 275 280 285

Leu Pro Thr Ala Glu Leu Glu Leu Lys Asp Met Arg Ala His Leu 290 295 300

Gly Glu lie Asp Gin Gly lie Val Thr lie Ala Pro Leu Leu Asn 310 315 320

Thr Arg Leu His Thr Phe Val Gly Ser Leu Ala Gly Trp Arg Arg 325 330 335 lie Ser lie Thr Lys Ser Phe Ala Lys Ala Arg Thr Thr Val Gly 340 345 350

Pro Leu Trp Leu lie Pro Met His Leu Arg Leu Leu Ala Asp Met 355 360 365

Val Lys His Arg Gly Ala Met Asn Leu Ala Trp Phe Thr Val Ala 370 375 380

Phe Gly Val Val Glu Asp Arg Thr Pro Ser Ser Asn Lys lie Ala 390 395 400

Leu Pro Gin Pro Gly Lys Glu Ala Glu Val Val Phe Arg Thr Leu 405 410 415

Ala Thr Ala Lys Ala Val lie Ser Lys Met Ala Thr Ala Gly lie 420 425 430

Glu Cys Gin Glu Ser Met Gly Gly Val Gly Tyr Met Asp Glu Ala 435 440 445

Glu Pro Glu Phe Asn lie Ser Arg lie Leu Arg Asn Asn Ala Val 450 455 460

Ser lie Trp Glu Gly Thr Thr Asn Val Leu Ala Ser Glu Phe Val 470 475 480 67

Arg Phe Leu lie Lys Lys Asp Asn Leu Lys lie Phe Gly Thr Trp Leu 485 490 495 Asp Arg Thr Leu Ala Leu lie Gin Ser Val Asp Leu Arg Asn Ala Leu 500 505 510 Thr Ala Ala Trp Leu Ala Leu His Ala Arg Phe Met Thr Gin Asp Pro 515 520 525 Ala Thr 530 Thr Val Ala Asp Gly Arg Arg Phe Met Phe Thr Val Ala Trp 535 540 lie Leu 545 Ser Gly Ala Leu Leu Ala Leu Asp Thr Glu Arg Asp Asn Asp 550 555 560 Pro Val Thr Ala Glu lie Ala Arg Arg Trp lie Leu Ser Ala Glu Gly 565 570 575 Gly Val Gly Glu Gin Val Phe His Asp lie Val Thr Val Ser Gly Thr 580 585 590 Ala Ser Ala Thr Ser Gly Gly Glu Glu His Leu Gin Trp Asp Cys Arg 595 600 605 He Ala 610 Trp Gly Val Asp Leu Pro Ala Asn Arg Ala Ser Gly His Arg 615 620

Ser Leu Gin Lys Ala Gly Ser Lys Leu 625 630 <210> 47 <211> 695

<212> PRT <213> Penicillium chrysogenum <400> 47

Met Pro 1 Ser Pro Pro Pro Asp Trp Val Lys Ala Leu Lys Pro Ser Gly 5 10 Pro Gin Gly Ser Glu Leu Leu His Gin Glu Arg Ala Gin Ser Asn Val 20 25 30 Asp Val Glu Arg Leu Ser Glu Leu Leu His Thr Lys Glu Thr Leu Glu 35 40 45 Arg Arg 50 Ala Ser Leu Leu Ala Leu Leu Gin Pro Glu Lys Val Phe Asp 55 60 Lys Ser 65 Gin Asn His Ser Leu Gly Arg Val Glu Arg Leu Gin Arg Ser 70 75 80 Leu Ala Lys Ala Lys Arg Leu Gin Gin Leu Ala Glu Glu His Lys Trp 85 90 95 Ser Met Gin Glu Leu His Ala Ala Asn Asp Leu lie Gly Glu Pro Thr 100 105 110 68

Pro Tyr Gly Leu His Ala Ser Met Phe Leu Val Thr Leu Arg Glu Gin 115 120 125

Gly Thr Pro Glu Gin His Lys Leu Phe Leu Glu Arg Ala Glu Lys Tyr 130 135 140

Glu lie lie Gly Cys Tyr Ala Gin Thr Glu Leu Gly His Gly Ser Asn 145 150 155 160

Val Arg Gly Leu Glu Thr Thr Ala Thr Trp Asn Ser Asn Asp Lys Thr 165 170 175

Phe Thr lie Asn Ser Pro Thr Leu Thr Ala Ser Lys Trp Trp lie Gly 180 185 190

Ser Leu Gly Arg Thr Ala Asn His Ala Val Val Met Ala Gin Leu Phe 195 200 205 lie Asp Gly Lys Asn Tyr Gly Pro His Pro Phe Val Val Gin Val Arg 210 215 220

Asp Leu Glu Thr His Gin Pro Leu Asp Asn Val Tyr Val Gly Asp lie 225 230 235 240

Gly Pro Lys Phe Gly Tyr Asn Thr Met Asp Asn Gly Phe Leu Leu Phe 245 250 255

Asn Asn Val Lys lie Pro His Val Asn Met Leu Ala Arg Phe Cys Gin 260 265 270

Val Asp Lys Glu Thr Asn Gin Tyr Ala Lys Pro Ala Met Pro Ser Leu 275 280 285

Val Phe Gly Thr Met Thr Trp Val Arg Ala Asn lie Val Leu Asp Ala 290 295 300

Gly Gly Val Leu Ala Arg Gly Val Thr lie Ala Thr Arg Tyr Cys Ala 305 310 315 320

Val Arg Arg Gin Phe Gin Asp Arg Asp Ala Asp Pro His Ala Gly Glu 325 330 335

Thr Gin Val Leu Asn Tyr Lys Met Val Gin Val Arg Leu Leu Pro Leu 340 345 350

Leu Ala Ser Met Tyr Ala Leu His Phe Thr Gly Arg Gly Met Met Arg 355 360 365

Leu Tyr Glu Glu Asn Gin Ser Arg Met Lys Ala Ala Ser Ser Pro Asp 370 375 380

Gin Glu Ser Arg Gly Ala Gly Pro Glu Gin Leu Arg Ala Gly Ala Asn 385 390 395 400

Leu Leu Ala Asp Leu His Ala Thr Ser Cys Gly Leu Lys Ala Leu Ala 405 410 415 69

Ser Thr Thr

Ala Gly Glu Gly Leu Glu lie Cys Arg Arg Ala Cys Gly 420 425 430

Gly His Gly 435

Tyr Ser Ser Tyr Ser Gly 440 lie Gly Pro Ala Tyr Ala Asp 445

Tyr Leu Pro 450

Thr Leu Thr Trp Glu Gly Asp Asn Tyr Met Leu Thr Gin 455 460

Gin Val Ala 465

Arg Tyr Leu Leu Lys Ser Ala Arg Ala Val Leu Ala Gly 470 475 480

Lys Pro Ala

Arg Asn Asp Thr Ser Gin lie Leu Gin Ala Tyr Leu Asp 485 490 495

Arg Arg Asp

Lys Gly Ala Ser Phe Asp Val Leu Asp Glu Asp Lys Asp 500 505 510 lie Val Ala 515

Ala Phe Gly Trp Arg Thr Ala His Leu Thr Phe Glu Ala 520 525

Leu Lys His 530

Arg Asp Ala Glu Gin Arg Ser Trp Asn Ser Leu Leu Val 535 540

Asp Phe Trp 545

Arg Leu Ser Thr Ala His Ser Gin Tyr Leu Met Val Lys 550 555 560

Asn Phe Tyr

Glu Ala Val Ser Ser Pro Glu Leu Ser Gly Ala Leu Asp 565 570 575

Pro Glu Thr

Lys Gly Leu Met His Gin Leu Phe Arg Leu Phe Ser Leu 580 585 590

His Thr Leu 595

Glu Arg Glu Ala Ala Glu Phe Phe Ser Ser Gly Ala Val 600 605

Thr Val Arg 610

Gin lie Thr Leu Thr Arg Thr Thr Ala Val Leu Lys Leu 615 620

Leu Asp Asp 625 lie Arg Pro His Ala Val Arg Leu Val Asp Ala Trp Ala 630 635 640 lie Pro Asp

Trp Gin Leu Asp Ser Ser Leu Gly Arg Tyr Asp Gly Lys 645 650 655

Val Tyr Glu

Asp Leu Phe Arg Arg Ala Ser Glu Glu Asn Pro Val Asn 660 665 670

Glu Leu Val 675

Phe Asp Pro Tyr Pro Trp Asn Ser Ala Leu Leu Lys Asn 680 685

Glu Pro Ala 690

Lys Ser Lys Leu 695 <210> 48 <211> 542 70 201033370

<212> PRT <213> Penicillium chrysogenum <400> 48

Mat Ala Lys Thr Phe Ser Lys Glu Asp Val Ala Ser His Ser Lys Gly 15 10 15

Asp Ser Pro Trp lie lie lie Asp Glu Asp Val Tyr Asp Val Ser Lys 20 25 30

Phe Gin Glu Glu His Pro Val Leu Gin Arg Val Ala Gly Lys Asp Ala 35 40 45

Ser Lys Gin Phe Trp Lys Tyr His Asn Glu Gly lie Leu Lys Lys Tyr 50 55 60

Lys Gly Gin Leu Gin lie Gly Ser Leu Asp Thr Lys Lys Ala Ala Pro 65 70 75 80

Ala Pro Pro Thr Pro Ala Pro Ala Pro Lys Lys Ala Ala Pro Glu Pro 85 90 95

Lys Ser Thr Ala Ser Ser Ser Ser Val Thr Pro Pro Ala Thr Gly Ala 100 105 110

Pro Gin Asp Pro Tyr Gly Glu Leu lie Pro Phe Ala Asp Pro Ser Trp 115 120 125

Tyr Gin Gly Tyr Ala Ser Pro Tyr Phe Asn Glu Ser His Ala Ala Leu 130 135 140

Arg Asp Glu Val Arg Gin Trp Val Glu Ser Glu lie Glu Pro Tyr Val 145 150 155 160

Thr Glu Trp Asp Glu Ala Lys Glu Val Pro Ala His lie Tyr Lys Gin 165 170 175

Met Gly Glu Arg Gly Tyr Leu Ala Gly Leu Leu Gly Val His Phe Pro 180 185 190

Glu Lys His Thr Pro His Arg Val Lys Ser Val Ser Pro Asp Arg Trp 195 200 205

Asp Leu Phe His Glu Met Leu Leu Thr Asp Glu Leu Ser Arg Ala Gly 210 215 220

Ser Gly Gly Leu Val Trp Ser Leu lie Gly Gly Tyr Gly lie Gly Cys 225 230 235 240

Pro Pro Leu Val Lys Phe Gly Lys Lys Pro Leu Val Asp Arg lie Leu 245 250 255

Pro Gly lie Leu Ala Gly Asp Lys Arg lie Cys Leu Ala lie Thr Glu 260 265 270

Pro Asp Ala Gly Ser Asp Val Ala Asn Leu Gly Cys Glu Ala Lys Leu 275 280 285 71

Thr Glu Asp Gly Lys His Tyr lie Val Asn Gly Glu Lys Lys Trp lie 290 295 300

Thr Asn Gly He Tyr Ser Asp Tyr Phe Thr Thr Ala Val Arg Thr Gly 305 310 315 320

Lys Asp Gly Met Asn Gly Leu Ser Val Leu Leu lie Glu Arg Glu Ala 325 330 335

Gly Gly Val Ser Thr Arg Arg Met Asp Cys Gin Gly Val Trp Ser Ser 340 345 350

Gly Thr Thr Tyr Val Thr Phe Glu Asp Val Lys Val Pro Val Glu Asn 355 360 365

Leu lie Gly Lys Glu Asn Gin Gly Phe Lys Val lie Met Thr Asn Phe 370 375 380

Asn His Glu Arg lie Gly lie Val lie Gin Cys Val Arg Phe Ser Arg 385 390 395 400

Val Cys Tyr Glu Glu Ser Met Lys Tyr Ala His Lys Arg Lys Thr Phe 405 410 415

Gly Lys Arg Leu lie Asp His Pro Val lie Arg Met Lys Leu Ala His 420 425 430

Met Ala Arg Gin lie Glu Ala Thr Tyr Asn Trp Leu Glu Asn lie lie 435 440 445

Phe Gin Cys Gin Ser Met Glu Asp Thr Glu Ala Met Leu Lys Leu Gly 450 455 460

Gly Ala lie Ala Gly Leu Lys Ala Gin Ser Thr Gin Cys Phe Glu Phe 465 470 475 480

Cys Ala Arg Glu Ala Ser Gin lie Phe Gly Gly Leu Ser Tyr Ser Arg 485 490 495

Gly Gly Gin Gly Gly Lys lie Glu Arg Leu Tyr Arg Asp Val Arg Ala 500 505 510

Tyr Ala lie Pro Gly Gly Ser Glu Glu lie Met Leu Asp Leu Ser Met 515 520 525 530 535 <210> 49 <211> 438 <212> PRT <213> Penicillium chrysogenum <400>

Arg Gin Ser Leu Arg Val His Gly Met Phe Gly Met Lys Leu

Met Ala Asp Thr Leu Arg Pro Ala Thr Ala Pro Tyr Ser Glu Pro Leu 15 10 15 72

Leu Pro Gin Leu Asp Val Arg Asn Pro Tyr Tyr Thr Asp Leu His His 20 25 30

Asn Leu Arg Ala Thr Val Arg Glu Tyr Val Asp Thr Tyr lie Ser Pro 35 40 45

Tyr Ala Ala Glu Trp Glu Glu Ala Gly Gin Val Pro Glu Ala Val Arg 50 55 60

Arg Arg His Cys Lys Leu Gly Tyr Ser lie Val His Pro Leu Thr Ser 65 70 75 80

Glu Glu Asp Ser Ala Gly lie Ser Leu Pro Gly Asn Val Pro Arg Glu 85 90 95

Lys Trp Asp Thr Trp Cys Ser Leu lie Val Ser Asp Glu Leu Thr Arg 100 105 110

Val Gly Tyr Val Gly Val lie Trp Gly Leu Gly Gly Gly Asn Gly lie 115 120 125

Gly Cys Pro Pro Val Ala Arg Phe Gly Asn Ala Glu Gin Arg Lys Lys 130 135 140

Trp Leu Pro Gly Val Ala Arg Gly Asp lie Arg Phe Cys Leu Gly lie 145 150 155 160

Thr Glu Pro Asp Ala Gly Ser Asp Val Ala Asn lie Gin Thr Thr Ala 165 170 175

Gin Arg Asp Gly Asn His Tyr Val Val Asn Gly Ser Lys Lys Trp lie 180 185 190

Thr Asn Gly lie Trp Ala Asp Tyr Cys Thr Ala Ala Val Arg Thr Gly 195 200 205

Gly Pro Gly Arg Ser Gly lie Ser Leu Leu Val lie Pro Leu Ala Thr 210 215 220

Ala Gly Val Thr Arg Arg Arg Met His Asn Ser Gly Val Asn Ala Ser 225 230 235 240

Gly Ser Thr Phe lie Glu Phe Glu Asp Val Arg Val Pro Val Glu Asn 245 250 255

Leu Val Gly Gin Glu Asn Lys Gly Phe Pro Leu lie Met Ser Asn Phe 260 265 270

Asn Pro Glu Arg Leu Ala Leu Ala Cys Ala Ser Leu Arg Leu Ala Arg 275 280 285

Val Cys Ala Glu Asp Ala Tyr Asn Tyr Ala lie Lys Arg Glu Thr Phe 290 295 300

Gly Ser Ala Leu lie Glu Lys Gin Ala lie Gin Ser Lys lie Phe Lys 73 305 310 315 320

Phe Gly Leu Met lie Glu Pro Ala Tyr Ala Phe Met Glu Gin Leu Val 325 330 335

Asn lie Leu Glu Leu Thr Lys Asp Arg Pro Ser Asp Asp Val Asn lie 340 345 350

Gly Gly Met Thr Ala Leu Leu Lys Val Met Ser Thr Arg Ala Leu Glu 355 360 365

Lys Ser Val Arg Glu Ala Gin Gin lie Met Gly Gly Ala Gly Tyr Asn 370 375 380

Lys Ala Gly Lys Gly Ala Arg lie Glu Gin lie Ser Arg Asp Ala Arg 385 390 395 400

Val His Val Val Gly Gly Gly Ser Glu Glu lie Met Ala Gly Leu Ala 405 410 415

Leu Arg Glu Glu Thr Lys Ala lie Arg Thr Arg Arg Lys Ala Leu Glu 420 425 430

Lys Arg Gin Ser Lys Val 435 <210> 50

<211> 453 <212> PRT <213> Penicillium chrysogenum <400> 50

Met Asn Phe Asp Leu Pro Ala Asp Leu Lys Thr His Leu Glu Ser lie 15 10 15

Asp Ser Phe lie His Ser Thr lie Leu Pro Leu Gin His Ser Asn Asp 20 25 30

Asn Asn Arg Phe Phe Asp His Arg Arg Glu Tyr Glu Arg Thr Asp Trp 35 40 45

Glu Asn Asn Gly Asn Pro Arg Lys Glu Trp Glu Glu Leu Leu Gly Glu 50 55 60

Ala Arg Thr Leu Ala Asp Ser Ser Gly Leu Tyr Arg Phe Ala Leu Pro 65 70 75 80

Arg Val Tyr Gly Gly Gin Ser His Pro Asp Val Asn Leu Trp Met Ser 85 90 95

Ala lie Arg Tyr His Leu Ser Ser Asn Ala Val Tyr Gly Gly Gly Leu 100 105 110

Gly Leu Ala Asn Asp Leu Gin Asn Glu His Cys lie Val Gly Asn Phe 115 120 125

Pro Asp Val Leu Met Leu His His Phe Gly Asn Glu Gin Gin Arg Asn 74 130 135 140

Thr Leu lie Pro Ala Arg Leu Arg Gly Glu Phe Arg Thr Thr Phe Gly 145 150 155 160

Leu Thr Glu Pro Asp His Gly Ser Asp Ala Thr Phe Met Ser Thr Thr 165 170 175

Ala Arg Pro Thr Arg Gly Gly Phe Glu lie Lys Gly Ala Lys Lys Trp 180 185 190

Gin Thr Gly Ala His His Cys Thr His Phe Leu lie Phe Ala Arg Thr 195 200 205

Ser Gly Lys Ala Gly Ser Ala Gin Gly lie Thr Ala Phe Leu Val Pro 210 215 220

Arg Asp Thr Arg Gly Val Arg lie Val Ser Tyr Glu Trp Thr Leu Asn 225 230 235 240

Met Pro Thr Asp His Ala Thr Val Glu Leu Asn Ser Val Trp Val Pro 245 250 255

Glu Ser Ala Val Leu Gly Ser lie Asp Gin Gly Leu Ala lie Ala Gin 260 265 270

Thr Phe Val His Glu Asn Arg lie Arg Gin Ala Ala Ser Ser Cys Gly 275 280 285

Ala Ala Arg Tyr Cys Leu Asp Arg Ser lie Asp Arg Ala Arg Ala Arg 290 295 300

Lys lie Trp Gly Glu Gly Lys Ser Leu Ala Asp Asn Gin Ala lie Gin 305 310 315 320

Phe Pro Val Val Glu Leu Met Thr Gin Val Glu Met Leu Arg Leu Phe 325 330 335 lie Leu Lys Thr Ser Trp Glu Met Asp Arg lie Val Ala Glu Cys Gin 340 345 350

Ser Ser Lys Ala Gin Arg Ala Pro Trp Val Glu lie Glu Gly Arg Leu 355 360 365

Ser Asp Gin Val Ala Met Cys Asn Phe Trp Ala Asn Arg Leu Cys Cys 370 375 380

Gin Ala Ala Asp Arg Ala lie Gin lie His Gly Gly Asp Gly Tyr Ser 385 390 395 400

Arg His Tyr Pro Phe Glu His lie Tyr Arg His Phe Arg Arg Tyr Arg 405 410 415 lie Thr Glu Gly Ala Glu Glu lie Gin Met Arg Lys lie Gly Ala Tyr 420 425 430 75

Lie Phe Gly Phe Ala 435 Ser Lys Ala Arg lie 450 Gly Pro Lys Lys Arg Glu Met Lys His Glu His 440 445 <210> 51 <211> 441 <212> PRT <213> Penicillium chrysogenum <400> 51

Met Ala Tyr Asn Ser 1 5 Pro Asn Pro lie Pro Phe Ser Glu Pro Pro Tyr 10 15 lie Arg Gly Leu Pro 20 Ser Pro Tyr lie Thr Pro Ala His Arg Arg Phe 25 30 Gin Gin Ala Cys Arg 35 Lys Phe Ala Thr Glu Asn Leu lie Gin His Ala 40 45 Leu Glu Trp Glu Arg 50 Glu Gly Thr Val Pro Glu His Val Phe His Thr 55 60 Phe Cys Lys His Asn 65 Met Leu Leu Pro Asn Met Pro Ala Pro Leu Pro 70 75 80 Val Asp Trp Leu Lys 85 Arg Leu Gly lie Asn Asp lie Leu Gly Val Lys 90 95 Val Glu Asp Trp Asp 100 Tyr lie Tyr Thr Gly lie Tyr Cys Asp Glu Met 105 110 Ala Arg Ser Gly Leu 115 Ser Gly Pro Ser Gly Ser Leu Asn Ala Gly Phe 120 125 Ala Phe Gly lie Ala 130 Pro lie Tyr Lys Phe Gly Ser Thr Glu Leu Gin 135 140 Glu Arg Phe Leu Pro 145 Glu Leu Leu Thr Gly Lys Lys Arg Gly Cys He 150 155 160 Ala lie Thr Glu Pro 165 Glu Ala Gly Ser Asp Val Ala Asn lie Thr Thr 170 175 Thr Ala Val Lys Ser 180 Ala Asp Gly Gin His Tyr lie Leu Asn Gly Ser 185 190 Lys Lys Trp lie Thr 195 Asn Gly lie Trp Ser Asp Tyr Ala Thr Met Ala 200 205

Val Arg Thr Gly Gly Pro Gly Ala Ala Gly Leu Ser Val Leu Val Val 210 215 220 Pro Leu Lys Gly His 225 Pro Gly Val Ser Met Arg Arg Leu Lys Val Ser 230 235 240 76 201033370

Gly Gin lie Thr Gly Gly Thr Thr Tyr lie Glu Leu Asp Asp Val Lys 245 250 255

Val Pro Val Ser Asn lie lie Gly Lys Glu Gly Asp Gly Met Arg lie 260 265 270 lie Met Thr Asn Phe Asn His Glu Arg Leu Val lie Ala Val Gly Val 275 280 285

Thr Arg Gin Ala Arg Val Ala Leu Ser Ala Ala Phe Ser Tyr Cys Leu 290 295 300

Lys Arg Glu Ala Phe Gly Lys Thr Leu Met Asp Gin Pro Val Val Arg 305 310 315 320

His Arg Leu Ala Lys Ala Gly Ala Glu Leu Glu Ser Met Trp Ala Trp 325 330 335

Val Glu Gin lie Leu Tyr Gin Leu Val His Leu Ser Lys Glu Glu Gly 340 345 350

Asp Arg Gin Leu Gly Gly Leu Thr Ala Leu Ala Lys Ala Lys Ser Ala 355 360 365

Met Val Leu Asn Glu Cys Ala Gin Thr Ala Val Leu Leu Phe Gly Gly 370 375 380

Asn Gly Phe Thr Lys Thr Gly Gin Gly Glu Leu Val Glu Ala lie Leu 385 390 395 400

Arg Asp Val Pro Gly Ala Arg lie Pro Gly Gly Ser Glu Asp Val Leu 405 410 415

Leu Asp Leu Ser Val Arg Gin Leu Val Lys Leu Tyr Gin Ala Glu Glu 420 425 430

Lys Lys Leu Ser Lys Asn Ala Lys lie 435 440 <210> 52 <211> 439 <212> PRT <213> Penicillium chrysogenum <400>

Met Tyr Arg Ser Gin lie Gin Arg Ala Leu Arg Ser Gin Trp Pro Ala 1 5 10 15

Leu Arg Gin Leu Gin Cys Arg Thr Gly Leu Ser Pro Arg Ala Arg Ala 20 25 30

Ala Phe Ser Thr Ser Gly Arg Arg Asp lie Met Gly Met Thr Gly Phe 35 40 45

Thr Asp Glu Gin Leu Thr Val Arg Glu Ala lie Ser His lie Cys Ser 50 55 60 77

Arg Phe Pro Asn Thr Tyr Trp Gin Glu Arg Asp Gin Gin Glu Lys Asp 65 70 75 80

Pro Lys Glu Phe His Ala Ala Leu Ala Lys Asp Gly Trp Leu Gly lie 85 90 95

Ala Leu Pro Glu Ser Leu Gly Gly Ala Gly Leu Gly lie Ser Glu Ala 100 105 110

Thr Met Met Met Gin Thr lie Thr Gin Ser Gly Ala Gly Met Ala Gly 115 120 125

Ala Gin Ala lie His Ala Asn Val Tyr Ala Thr Gin Pro Leu Ala Lys 130 135 140

Phe Gly Thr Lys Glu Gin Leu Glu Thr Thr lie Pro Asn lie lie Asn 145 150 155 160

Gly Thr Trp Arg Thr Cys Phe Gly Val Thr Glu Pro Asn Thr Gly Leu 165 170 175

Glu Thr Leu Lys Leu Thr Thr Leu Ala Ser Lys Thr Asp Asp Gly Tyr 180 185 190

Ser Val Thr Gly Gin Lys lie Trp lie Thr Cys Ala Gin Val Ala Ser 195 200 205

Lys Met lie Leu Leu Ala Arg Thr Thr Pro Leu Glu Glu Val Lys Lys 210 215 220

Ser Ser Glu Gly Leu Ser Leu Phe Cys lie Asp lie Asp Arg Glu Asn 225 230 235 240

Pro Gly Leu Asp Leu Arg Lys lie Lys Lys Met Gly Gly Arg Ala Val 245 250 255

Asp Ala Asn Glu Val Phe Phe Asp His Tyr Lys lie Pro Ala Asn Thr 260 265 270

Leu lie Gly Glu Glu Asn Gin Gly Phe Lys lie lie Leu His Gly Met 275 280 285

Asn Ala Glu Arg Cys Leu Leu Ala Gly Glu Ala Leu Gly Leu Gly Tyr 290 295 300

Ala Ala Leu Glu Lys Ala Ser Gin Tyr Ala Lys Asp Arg Val Val Phe 305 310 315 320

Gly Arg Pro lie Gly Gin Asn Gin Gly Val Ala His Pro Leu Ala Asp 325 330 335

Ala Phe Met Lys Leu Glu Ala Ala Lys Leu Ala Thr Tyr His Ala Ala 340 345 350

Arg Leu Tyr Asp Thr Asn Asp Gly Ser Val Pro Phe His Glu lie Gly 355 360 365 78

Val Ala Cys Asn Ser Ala Lys Tyr Leu Ala Ala Glu Ala Ala Phe Thr 370 375 380

Ala Cys Glu Arg Ala Val Leu Ala His Gly Gly Met Gly Tyr Ala Val 385 390 395 400

Glu Tyr Asp Val Glu Arg Tyr Met Arg Glu Cys Phe Val Pro Arg lie 405 410 415

Ala Pro Val Ser Arg Glu Met lie Leu Asn Tyr Val Ser Glu Lys Val 420 425 430

Leu Asp Leu Pro Arg Ser Tyr 435

<210> 53 <211> 446 <212> PRT <213> Penicillium chrysogenum <400> 53

Met Ser Ala Ser Ser Arg lie Pro Pro lie Ala Gin Pro Phe Val Ser 15 10 15

Glu His Ala Lys Arg Thr Leu Asp Leu Val Glu Glu Phe Val Glu Lys 20 25 30

Asp Cys lie Pro Ala Asp Thr Val Phe Ser Ala Gin Leu Gly Glu Gly 35 40 45

Glu Lys Arg Trp Thr Thr Thr Pro Thr Val Leu Glu Gly Leu Lys Glu 50 55 60

Lys Ala Lys Lys Leu Gly Leu Trp Asn Met Phe Leu Pro Lys Asn His 65 70 75 80

Phe Thr Gin Gly Ala Gly Phe Ser Asn Leu Glu Tyr Gly Leu Met Ala 85 90 95

Glu Leu Leu Gly Lys Ser Lys Val Ala Ser Glu Ala Thr Asn Asn Ala 100 105 110

Ala Pro Asp Thr Gly Asn Met Glu Val Phe Ala Lys Tyr Gly Asn Asp 115 120 125

Ala Gin Lys Lys Gin Trp Leu Ala Pro Leu Leu Glu Gly Lys lie Arg 130 135 140

Ser Ala Phe Leu Met Thr Glu Pro Asp Val Ala Ser Ser Asp Ala Thr 145 150 155 160

Asn lie Glu Leu Asn lie Arg Arg Glu Gly Asn Glu Tyr Val Leu Asn 165 170 175

Gly Ser Lys Trp Trp Ser Ser Gly Ala Gly Asp Pro Arg Cys Ala lie 180 185 190 79

Tyr Leu Val Met Gly Lys Thr Asp Pro Thr Asn Pro Asp Thr Tyr Lys 195 200 205 Gin Gin 210 Ser Val lie Leu Val Pro Ala Gly Leu Pro Gly lie Thr Val 215 220 His Arg 225 Met Leu Thr Val Tyr Gly Tyr Asp Asp Ala Pro His Gly His 230 235 240 Gly His lie Thr Phe Lys Asp Val Arg Val Pro Ala Ser Asn Met Val 245 250 255 Leu Gly Glu Gly Arg Gly Phe Glu lie lie Gin Gly Arg Leu Gly Pro 260 265 270 Gly Arg He His His Ala Met Arg Ala lie Gly Ala Ala Glu Arg Ala 275 280 285 Leu Glu 290 Trp Leu lie Ala Arg Val Asn Asp Glu Arg Lys Met Thr Phe 295 300 Gly Lys 305 Pro Leu Val Ala His Gly Val lie Leu Glu Trp lie Ala Lys 310 315 320 Ser Arg lie Glu Val Asp Ala Ala Arg Leu lie Val Leu Asn Ala Ala 325 330 335 lie Lys lie Asp Gin Gly Asp Ala Lys Ser Ala Leu Lys Glu lie Ala 340 345 350 Gin Ala Lys Val Leu Val Pro Gin Thr Ala Leu Thr lie lie Asp Arg 355 360 365 Ala Val 370 Gin Ala Tyr Gly Ala Ala Gly Val Cys Gin Asp Thr Pro Leu 375 380 Ala Tyr 385 Leu Trp Ala Gly lie Arg Thr Leu Arg lie Ala Asp Gly Pro 3 90 395 400 Asp Glu Val His Leu Gin Gin Leu Gly Lys Arg Glu Asn Lys Ala Arg 405 410 415 Lys Asp Ala Val Thr Ala Lys Leu Asn Trp Gin Arg Glu Glu Ala Asp 420 425 430 Arg Leu Leu Ala Ala Ser Gly Phe Lys Pro Lys Ser His Leu 435 440 445 <210> 54 <211> 513

<212> PRT <213> Penicillium chrysogenum <400> 54

Met Pro Ser Glu Thr Leu Thr Arg Ala Glu Val Ala Lys His Asn Thr 15 10 15 80

Glu Asp Ser Leu Trp Cys lie lie Asp His Gin Val Tyr Asp Leu Thr 20 25 30

Asp Phe Val Asp Ala His Pro Gly Gly Gly Val Val Leu Ala Gin Val 35 40 45

Ala Gly Lys Asp Ala Thr Ser Asp Phe Tyr Asn Leu His Arg Gin Glu 50 55 60

Val Leu Glu Lys Tyr Arg Asp Gin Leu Cys lie Gly Val Val Glu Gly 65 70 75 80

Glu Lys Pro Glu Val lie Arg Pro Phe Pro Gly Ala Leu Ser Pro Val 85 90 95

Pro Tyr Ala Glu Pro Leu Trp Leu Arg Pro Gin Phe Lys Ser Pro Tyr 100 105 110

Tyr Lys Glu Thr His Arg Asn Leu Gin Lys Ala lie Arg Glu Phe Thr 115 120 125

Asp Lys Tyr Val Thr Pro Glu Ala Gin Glu Lys Glu Gin Asp Gly Ser 130 135 140

Tyr lie Ser Gin Glu Leu lie Asn Arg Met Ala Glu Thr Asn lie Leu 145 150 155 160

Ala Met Arg Leu Gly Pro Gly Lys His Leu His Gly Arg Thr Leu Leu 165 170 175

Gly Gly Val Val Asp Gly Lys Glu Phe Asp Tyr Leu His Asp Met lie 180 185 190 lie Val Gin Glu Met Val Arg Ala Asn Ala Arg Gly Phe Gin Asp Gly 195 200 205

Asn Met Ala Gly Met Ala lie Ser Leu Thr Ala Val Gin Gin Trp Leu 210 215 220

His Asp Pro Val Leu Lys Glu Arg Leu Asn Asp Glu Val Leu Ser Gly 225 230 235 240

Arg Lys Lys Met Cys Leu Ala lie Thr Glu Ala Phe Ala Gly Ser Asp 245 250 255

Val Ala Gly Leu Lys Thr Thr Ala Glu Lys Thr Pro Asp Gly Lys His 260 265 270

Tyr lie Val Asn Gly Thr Lys Lys Trp lie Thr Asn Gly Met Phe Ala 275 280 285

Asp Tyr Phe Val Thr Gly Cys Arg Thr Glu Lys Gly Phe Ser Val Leu 290 295 300

Leu lie Pro Arg Gly Glu Gly Val Glu Thr Lys Gin lie Lys Thr Ser 305 310 315 320 81

Tyr Ser Thr Ala Ala Ala Thr Ala Phe Val Glu Phe Asp Asn Val 325 330 335

Val Pro Val Gin Asn Leu Leu Gly Glu Glu His Lys Gly Phe lie 340 345 350 lie Met Ser Asn Phe Asn His Glu Arg Phe Met Met Val Ala Ala 355 360 365

Val Arg Met Ser Met Met Val Val Glu Glu Thr Met Lys Trp Ser 370 375 380

Gin Arg lie Val Phe Gly Lys Lys Leu lie Glu Gin Pro Val lie 385 390 395

Gin Lys lie Ala Arg Met lie Ser Leu Ala Glu Ser Asn Gin Ala 405 410 415

Leu Glu Ser lie Ala Tyr Gin Met Cys Asn Met Thr Tyr Ala Gin 420 425 430

Ala Lys Leu Leu Gly Gly Pro lie Gly Leu Leu Lys Ser His Cys 435 440 445

Gin Ala Ala Gly Glu 450 lie Ala Ser Leu Ala Thr Asn lie Phe Gly 455 460

Arg Gly Leu Thr Gin 465

Ser Gly Met Gly Lys Val lie Glu Met Phe 470 475

Arg Thr Tyr Lys Phe Asp Ala lie Leu Gly Gly Thr Glu Glu lie 485 490 495

Ala Asp Leu Gly Val Arg Gin Ala 500 Leu <210><2X1><212><213> 55 436 PRT Penicillium chrysogenum <400> 55 505 510

Met Ala Ser lie lie Arg Ala Leu Arg Pro Leu Ser Arg Asn Pro 15 10 15

Val Arg Leu Ala Gly Lys Arg Leu Ala Ala Gly Arg Pro Val Gin 20 25 30

Ala Tyr Ala Phe 35

Ser Thr Thr Pro Arg Arg Arg Glu Val Asp Leu 40 45

Glu Leu Thr Pro Thr 50

Pro lie Thr Leu Leu Ser Glu Thr Glu Ser 55 60

Lys Val Val Asn Arg 400 Trp Gin Thr Gly His 480 Leu Met

Ser Ser Ser Leu 82

Met Ala Asp Ser Val Ser Lys Phe Ala Val Glu Gin lie Gly Pro Lys 65 70 75 80

Val Arg Glu Met Asp Glu Ala Glu Thr Met Asp Ala Lys Val Val Glu 85 90 95

Gin Leu Phe Glu Gin Gly Leu Met Gly lie Glu Val Pro Glu Glu Phe 100 105 110

Gly Gly Ala Gly Met Asn Phe Thr Ala Ala lie Val Ala lie Glu Glu 115 120 125

Leu Ala Arg Val Asp Pro Ser Val Ser Val Leu Val Asp Val His Asn 130 135 140

Thr Leu Val Asn Thr Ala lie Met Lys Tyr Gly Asp Ala Lys Ala Gin 145 150 155 160

Arg Thr Trp Leu Pro Lys Leu Thr Thr Gly Thr Val Gly Ser Phe Cys 165 170 175

Leu Ser Glu Pro Ala Ser Gly Ser Asp Ala Phe Ala Leu Gin Thr Lys 180 185 190

Ala Glu Lys Thr Ala Asp Gly Tyr Lys Leu Asn Gly Ser Lys Met Trp 195 200 205 lie Thr Asn Ser Met Glu Ala Gly Val Phe lie Val Phe Ala Asn lie 210 215 220

Asp Pro Ser Lys Gly Tyr Lys Gly lie Thr Ala Phe lie Val Glu Lys 225 230 235 240

Asp Thr Pro Gly Phe Ser lie Ala Lys Lys Glu Lys Lys Leu Gly lie 245 250 255

Arg Ala Ser Ser Thr Cys Val Leu Asn Phe Asp Asp Cys Val lie Pro 260 265 270

Lys Ser Asn Leu Leu Gly Glu Glu Gly Gin Gly Tyr Lys Tyr Ala lie 275 280 285

Ser Val Leu Asn Glu Gly Arg lie Gly lie Ala Ala Gin Met Thr Gly 290 295 300

Leu Ala Leu Gly Ala Trp Glu Asn Ala Ala Arg Tyr Val Trp Asn Asp 305 310 315 320

Arg Arg Gin Phe Gly Glu Leu lie Gly Asn Phe Gin Gly Met Gin His 325 330 335

Gin lie Ala Gin Ala Tyr Thr Glu lie Ala Ala Ala Arg Ala Leu Val 340 345 350

Tyr Asn Ala Ala Arg Lys Lys Glu Ala Gly Gin Asp Phe Val Gin Asp 83 355 360 365

Ala Ala Met Ala Lys Leu Tyr Ala Ser Gin Val Ala Gly Arg Val Ser 370 375 380

Ser Ser Ala Val Glu Trp Met Gly Gly Met Gly Phe Val Arg Glu Gly 385 390 395 400 lie Ala Glu Lys Met Phe Arg Asp Ser Lys lie Gly Ala lie Tyr Glu 405 410 415

Gly Thr Ser Asn He Gin Leu Gin Thr lie Ala Lys Leu Leu Gin Lys 420 425 430

Gin Tyr Thr Asn 435 <210> 56 <211> 429 <212> PRT <213> Penicillium chrysogenum <400> 56

Met Ala Ser Leu Thr Leu Pro Ser Leu Leu Arg Thr Ser Thr Arg Ala 15 10 15

Val Arg Leu Asn Arg Thr Pro Ala Leu Thr Pro Cys Phe Arg Ser lie 20 25 30

Ser Thr Lys His Pro Lys Gly Phe lie Pro Pro Ser Glu Asp Asp Leu 35 40 45

Leu Glu Leu Arg Glu Arg Val Gin Asp Phe Thr Arg Arg Glu lie Pro 50 55 60

Ala Asp Val Ala Ala Arg Thr Asp Glu Gin Asn Glu Phe Pro Ala Glu 65 70 75 80

Met Trp Arg Lys Met Gly Asp Ala Gly Phe Leu Gly Val Thr Ala Asn 85 90 95

Glu Glu Tyr Gly Gly Leu Gly Met Gly Tyr Gin Ala His Cys Val Val 100 105 110

Met Glu Glu lie Ser Arg Ala Ser Gly Ser He Gly Leu Ser Tyr Ala 115 120 125

Ala His Ser Gin Leu Cys Val Asn Gin Leu Ser Leu Asn Gly Ser Thr 130 135 140

Glu Gin Lys Glu Arg lie Leu Pro Gly Leu Leu Ser Gly Glu Lys Val 145 150 155 160

Gly Ala Leu Ala Met Ser Glu His Ser Ala Gly Ser Asp Val Val Ser 165 170 175

Met Lys Thr Thr Ala Lys Glu Val Asp Gly Gly Trp Leu Leu Asn Gly 84 180 185 190

Thr Lys Met Trp lie Thr Asn Gly Pro Asp Ala Asp Tyr lie Val Val 195 200 205

Tyr Ala Lys Thr Glu Pro Glu Leu Gly Ser Lys Gly lie Thr Ala Phe 210 215 220

Leu Val Glu Lys Asp Phe Lys Gly Phe Ser Cys Ala Arg Lys Leu Asp 225 230 235 240

Lys Leu Gly Met Arg Gly Ser Asn Thr Gly Glu Leu lie Phe Glu Asp 245 250 255

Val Phe Val Pro Arg Glu Asn Leu Leu Gly Glu Val Asn Arg Gly Val 260 265 270

Arg Val Leu Met Glu Gly Leu Asp Leu Glu Arg Leu Val Leu Ser Ala 275 280 285

Gly Pro Leu Gly lie 290

Met Gin Ala Ala Leu Asp Leu Val Leu Pro Tyr 295 300

Thr His Val Arg Lys Gin Phe Gly Ala Pro lie Ala His Asn Gin Leu 305 310 315 320

Val Gin Gly Lys Leu Ala Asp Met Tyr Thr Lys Leu Ala Ala Ser Arg 325 330 335

Ala Tyr Thr Tyr Ala Thr Ala Arg Gin Val Asp Asn Ala Ala Val Glu 340 345 350

Pro Gly Glu Leu Thr Val Arg Thr Gin Asp Cys Ala Gly Ala lie Leu 355 360 365

Tyr Ala Ala Glu Arg Ala Thr Glu Cys Thr Leu Asp Ala lie Gin Leu 370 375 380

Met Gly Gly Ser Gly Tyr lie Asn Glu lie Pro Ala Gly Arg Leu Leu 385 390 395 400

Arg Asp Ala Lys Leu Tyr Glu lie Gly Ala Gly Thr Ser Glu lie Arg 405 410 415

Arg Met Val lie Gly Arg Ala Phe Asn Lys Glu Tyr Ala 420 425 <210> 57 <211> 405

<212> PRT <213> Penicillium Chrysogenum <400> 57

Met

Phe Ser Arg Ser Val 5

Leu Gin Thr Ala Thr Arg Ser Val Arg Pro 10 15

Ala Ser Ala Arg Ala Val Pro Gin Leu Gly Lys Ala Pro Phe Gly Arg 85 20 25 30

Arg Tyr Val Ser Val Tyr Gly Tyr Thr Gin Ala Lys Ala Leu lie Tyr 35 40 45

Ser Lys Tyr Gly Glu Pro Lys Asp Val Leu Arg Leu His Lys His Ser 50 55 60 lie Ser Ala Pro His Ala Thr Gin Val Asn Leu Arg Leu Leu Thr Ala 65 70 75 80

Pro Met Asn Pro Ala Asp Val Asn Gin lie Gin Gly Val Tyr Pro Ser 85 90 95

Lys Pro Pro Phe Gin Thr Glu Leu Gly Asn Val Glu Pro Ala Ala Val 100 105 110

Gly Gly Asn Glu Gly Ala Phe Glu Val Leu Ser Thr Gly Ala Gly Val 115 120 125

Lys Asn Leu Ser Lys Gly Asp Trp Val lie Met Lys Arg Thr Gly Leu 130 135 140

Gly Thr Trp Arg Thr His Ala Gin Leu Asp Glu Ser Gin Leu lie Lys 145 150 155 160

Val Glu Asn Lys Glu Gly Leu Thr Pro Leu Gin Val Gly Thr Val Ser 165 170 175

Val Asn Pro Val Thr Ala Tyr Arg Met Leu Arg Asp Phe Cys Glu Trp 180 185 190

Asp Trp Met Arg Ala Gly Glu Glu Trp Val lie Gin Asn Gly Ala Asn 195 200 205

Ser Gly Val Gly Arg Ala Ala lie Gin Leu Gly Arg Glu Trp Gly lie 210 215 220

Lys Thr Leu Asn Val lie Arg Gin Arg Lys Thr Pro Glu Glu Thr Glu 225 230 235 240

Ala Leu Lys Gin Glu Leu Arg Asp 245

Leu Gly Ala Thr Val Val lie Thr 250 255

Glu Glu Glu Met Leu Asn Gly Asn 260

Phe Arg Asp Met Val His Glu Phe 265 270

Thr Arg Lys Gly Arg Glu Pro lie 275 280

Arg Leu Ala Leu Asn Cys Val Gly 285

Gly Lys Asn Ala Thr Ala Leu Ala 290 295

Lys Thr Leu Ala Pro Asp Ser His 300

Met Val Thr Tyr Gly Ala Met Ser 305 310

Lys Gin Pro Val Ala Leu Pro Ser 315 320 86

Gly Leu Leu lie Phe Lys Asn Leu Ala Phe Asp Gly Phe Trp Val Ser 325 330 335

Lys Trp Gly Asp Lys Asn Pro Gin Leu Lys Glu Ser Thr lie Lys Asp 340 345 350

Val Leu Gin Leu Thr Arg Ser Gly Lys Phe Lys Asp lie Pro Val Asp 355 360 365

Glu Val Lys Trp Asn Trp Glu Thr Glu Gly Pro Glu Leu Ala Glu Ser 370 375 380

Val Gin Gly Thr Leu Gly Gly Tyr Arg Ser Gly Lys Gly Val Phe Thr 385 390 395 400

Phe Ser Gly Gly Asp 405 <210> 58 <211> 373

<212> PRT <213 > Bos taurus <400> 58

Met Trp Val Cys Gly Ala Leu Cys Arg Thr Arg Ala Pro Ala Gin Leu 15 10 15

Gly Gin Arg Leu Leu Pro Glu Ser Arg Arg Arg Arg Pro Ala Ser Ala 20 25 30

Ser Phe Ser Ala Ser Ala Glu Pro Ser Arg Val Arg Ala Leu Val Tyr 35 40 45

Gly His His Gly Asp Pro Ala Lys Val Val Glu Leu Lys Asn Leu Glu 50 55 60

Leu Ala Ala Val Gly Gly Ser His Val His Val Lys Met Leu Ala Ala 65 70 75 80

Pro lie Asn Pro Ser Asp lie Asn Met lie Gin Gly Asn Tyr Gly Leu 85 90 95

Leu Pro Gin Leu Pro Ala Val Gly Gly Asn Glu Gly Val Gly Gin Val 100 105 110

Val Ala Val Gly Ser Gly Val Thr Gly Val Lys Pro Gly Asp Trp Val 115 120 125 lie Pro Ala Asn Pro Gly Leu Gly Thr Trp Arg Thr Glu Ala Val Phe 130 135 140

Gly Glu Glu Glu Leu lie Thr Val Pro Ser Asp lie Pro Leu Gin Ser 145 150 155 160

Ala Ala Thr Leu Gly Val Asn Pro Cys Thr Ala Tyr Arg Met Leu Val 165 170 175 87

Asp Phe Glu

Arg Leu Arg Pro Arg Asp Ser lie lie Gin Asn Ala Ser 180 185 190

Asn Ser Gly 195

Val Gly Gin Ala Val lie Gin lie Ala Ala Ala Arg Gly 200 205

Leu Arg Thr 210 lie Asn Val Leu Arg Asp Thr Pro Asp Leu Gin Lys Leu 215 220

Thr Asp Thr 225

Leu Lys Asn Leu Gly Ala Asn His Val Val Thr Glu Glu 230 235 240

Glu Leu Arg

Lys Pro Glu Met Lys Ser Phe Phe Lys Asp Val Pro Gin 245 250 255

Pro Arg Leu

Ala Leu Asn Cys Val Gly Gly Lys Ser Ser Thr Glu Leu 260 265 270

Leu Arg His 275

Leu Ala Pro Gly Gly Thr Met Val Thr Tyr Gly Gly Met 280 285

Ala Lys Gin 290

Pro Val lie Ala Ser Val Ser Gin Leu lie Phe Lys Asp 295 300

Leu Lys Leu 305

Arg Gly Phe Trp Leu Ser Gin Trp Lys Lys Asp His Ser 310 315 320

Pro Asp Gin

Phe Lys Glu Leu lie Leu Thr Leu Cys Asp Leu lie Arg 325 330 335

Arg Gly Gin

Leu Thr Ala Pro Ala Cys Ser Glu Val Pro Leu Gin Asp 340 345 350

Tyr Leu Cys 355

Ala Leu Glu Ala Ser Thr Gin Pro Phe Val Ser Ser Lys 360 365

Gin lie Leu 370

Thr Met <210> 59 <211> 302 <212> PRT <213> Cavia <400> 59 Met Gly Ser 1 sp .

Trp Thr Lys Cys Gin Ser Cys Leu Ala Pro Gly Leu Leu 5 10 15

Gin Asn Arg

Ala Ala lie Val Thr Gly Gly Gly Thr Gly lie Gly Lys 20 25 30

Ala lie Ala 35

Lys Glu Leu Leu His Leu Gly Cys Asn Val Val lie Ala 40 45

Ser Arg Lys 50

Phe Asp Arg Leu Arg Ala Ala Ala Glu Glu Leu Lys Ala 55 60 88

Thr Leu Pro Pro Ser Asn Lys Ala Glu Val Thr Pro lie Gin Cys Asn 65 70 75 80 lie Arg Lys Glu Glu Glu Val Asn Asn Leu Met Lys Ser Thr Leu Ala 85 90 95

Leu Tyr Gly Lys lie Asp Phe Leu Val Asn Asn Gly Gly Gly Gin Phe 100 105 110

Trp Ser Ser Pro Glu His lie Ser Ser Lys Gly Trp His Ala Val lie 115 120 125

Glu Thr Asn Leu Thr Gly Thr Phe Tyr Met Cys Lys Ala Ala Tyr Asn 130 135 140

Ser Trp Met Lys Glu His Gly Gly Ala lie Val Asn lie lie lie Leu 145 150 155 160

Leu Asn Gly Gin Pro Phe Val Ala His Ser Gly Ala Ala Arg Gly Gly 165 170 175

Val Tyr Asn Leu Thr Lys Ser Leu Ala Leu Gly Trp Ala Arg Ser Gly 180 185 190 lie Arg lie Asn Cys Val Ala Pro Gly Thr Val Tyr Ser Gin Thr Ala 195 200 205

Met Asp Asn Tyr Gly Asp Met Gly Lys Thr Leu Phe Ala Asp Ala Phe 210 215 220

Gin Lys lie Pro Ala Lys Arg Leu Gly Val Pro Glu Glu Val Ser Ser 225 230 235 240

Leu Val Cys Phe Leu Leu Ser Pro Ala Ala Ser Phe lie Thr Gly Gin 245 250 255

Leu Val Asn Val Asp Gly Gly Gin Ser Leu Tyr Cys Gin Asn His Asp 260 265 270 lie Pro Asp His Asp Asn Trp Pro Glu Gly Val Gly Asp Leu Ser Thr 275 280 285

Val Lys Lys Met Lys Glu Ser Phe Lys Gin Lys Ala Lys Leu 290 295 300 <210> 60 <211> 386

<212> PRT <213> Candida tropicalis <400> 60

Met Tyr Ser Val Leu 1 5

His Asn Gin Phe Arg 20

Lys Gin Ser lie Arg Pro Arg Leu Leu Ala Thr 10 15

Thr Met lie Thr Ala Gin Ala Val Leu Tyr Thr 25 30 89

Gin His Gly Glu Pro Lys Asp Val Leu Phe Thr Gin Ser Phe Glu lie 35 40 45 Asp Asp 50 Asp Asn Leu Ala Pro Asn Glu Val lie Val Lys Thr Leu Gly 55 60 Ser Pro 65 Val Asn Pro Ser Asp lie Asn Gin lie Gin Gly Val Tyr Pro 70 75 80 Ser Lys Pro Ala Lys Thr Thr Gly Phe Gly Thr Thr Glu Pro Ala Ala 85 90 95 Pro Cys Gly Asn Glu Gly Leu Phe Glu Val lie Lys Val Gly Ser Asn 100 105 110 Val Ser Ser Leu Glu Ala Gly Asp Trp Val lie Pro Ser His Val Asn 115 120 125 Phe Gly 130 Thr Trp Arg Thr His Ala Leu Gly Asn Asp Asp Asp Phe lie 135 140 Lys Leu 145 Pro Asn Pro Ala Gin Ser Lys Ala Asn Gly Lys Pro Asn Gly 150 155 160 Leu Thr lie Asn Gin Gly Ala Thr lie Ser Val Asn Pro Leu Thr Ala 165 170 175 Tyr Leu Met Leu Thr His Tyr Val Lys Leu Thr Pro Gly Lys Asp Trp 180 185 190 Phe lie Gin Asn Gly Gly Thr Ser Ala Val Gly Lys Tyr Ala Ser Gin 195 200 205 He Gly 210 Lys Leu Leu Asn Phe Asn Ser lie Ser Val lie Arg Asp Arg 215 220 Pro Asn 225 Leu Asp Glu Val Val Ala Ser Leu Lys Glu Leu Gly Ala Thr 230 235 240 G In Val lie Thr Glu Asp Gin Asn Asn Ser Arg Glu Phe Gly Pro Thr 245 250 255 lie Lys Glu Trp lie Lys Gin Ser Gly Gly Glu Ala Lys Leu Ala Leu 260 265 270 Asn Cys Val Gly Gly Lys Ser Ser Thr Gly lie Ala Arg Lys Leu Asn 275 280 285 Asn Asn 290 Gly Leu Met Leu Thr Tyr Gly Gly Met Ser Phe Gin Pro Val 295 300 Thr lie 305 Pro Thr Ser Leu Tyr lie Phe Lys Asn Phe Thr Ser Ala Gly 310 315 320 Phe Trp Val Thr Glu Leu Leu Lys Asn Asn Lys Glu Leu Lys Thr Ser 325 330 335 90 201033370

Thr Leu Asn Gin lie lie Ala Trp Tyr Glu Glu Gly Lys Leu Thr Asp 340 345 350

Ala Lys Ser lie Glu Thr Leu Tyr Asp Gly Thr Lys Pro Leu His Glu 355 360 365

Leu Tyr Gin Asp Gly Val Ala Asn Ser Lys Asp Gly Lys Gin Leu lie 370 375 380

Thr Tyr 385 <210> 61 <211> 379 <212> PRT <213> Clostridium kluyveri <400> 61 眷

Met Asp Phe Thr Leu Thr Asn Glu Gin Lys Phe Val Glu Gin Met Val 1 5 10 15

Ser Glu Phe Thr Glu Asn Glu Val Lys Pro lie Ala Ala Glu lie Asp 20 25 30

Glu Thr Glu Arg Phe Pro Leu Glu Thr Val Glu Lys Phe Ala Lys Tyr 35 40 45

Gly Met Met Gly Met Pro Phe Pro Val Glu Tyr Gly Gly Ser Gly Thr 50 55 60

Asp Tyr Leu Ser Tyr lie lie Ala Val Glu Gly Leu Ala Lys Ser Cys 65 70 75 80

Thr Ser Ser Ser Thr lie Leu Ser Ala His Thr Ser Leu Cys Ala Ala 85 90 95

Pro lie Tyr Asp Trp Gly Thr Glu Glu Gin Lys Gin Lys Tyr Leu Val 100 105 110

Pro Leu Ala Lys Gly Glu Lys Leu Gly Ala Phe Gly Leu Thr Glu Pro 115 120 125

Asn Ala Gly Thr Asp Ala Ala Gly Gin Gin Thr Thr Ala Val Leu Glu 130 135 140

Gly Asp His Tyr Val Leu Asn Gly Gin Lys lie Phe lie Thr Asn Gly 145 150 155 160

Ala Tyr Ala Asp Thr Phe Val lie Phe Ala Met Thr Asp Arg Ser Lys 165 170 175

Gly Thr Arg Gly lie Thr Ala Phe He Val Glu Lys Asp Phe Pro Gly 180 185 190

Phe Ser lie Gly Lys Ser Glu Asp Lys Leu Gly lie Arg Ala Ser Ser 195 200 205 91

Thr Thr Glu Leu lie Phe Glu Asn Cys lie Val Pro Lys Glu Asn Met 210 215 220

Leu Gly Lys Glu Gly Lys Gly Phe Thr Val Ala Met His Thr Leu Asp 225 230 235 240

Gly Gly Arg lie Gly lie Ala Ala Gin Ala Leu Gly Leu Ala Glu Gly 245 250 255

Ala Leu Ala Glu Ala Leu Asn Tyr Met Lys Glu Arg Lys Gin Phe Gly 260 265 270

Lys Ala Leu Tyr Lys Phe Gin Gly Leu Ala Trp Met Val Ala Glu Leu 275 280 285

Asp Thr Lys lie Glu Ala Val Lys Gin Leu Val Tyr Lys Ala Ala Val 290 295 300

Asn Lys Gin Met Gly Leu Pro Tyr Ser Val Glu Ala Ala Arg Ala Lys 305 310 315 320

Leu Ala Ala Ala Thr Val Ala Met Glu Thr Thr Thr Lys Val Val Gin 325 330 335 lie Phe Gly Gly Tyr Gly Phe Thr Lys Asp Tyr Pro Val Glu Arg Met 340 345 350

Met Arg Asp Ala Lys lie Thr Glu lie Tyr Glu Gly Thr Ser Gin Val 355 360 365

Gin Lys Met Val lie Ser Ala Asn Leu Phe Lys 370 375

<210> 62 <211> 562 <212> PRT <213> Clostridium kluyveri <400> 62

Met Asn Phe Glu Leu Thr Lys Glu Gin Gin Met lie Arg Asp Asn Val 15 10 15

Arg Lys Phe Ala Glu Ala Lys lie Glu Pro lie Ala Phe Gin Leu Asp 20 25 30

Glu Lys Asn lie Phe Pro Glu Glu lie Val Asn Glu Met Gly Asp Leu 35 40 45

Ser lie Met Gly Leu Pro Tyr Pro Lys Glu Tyr Gly Gly Ala Gly Lys 50 55 60

Asp Val Leu Ser Tyr Ala lie Ala Val Glu Glu Leu Ser Arg Val Asp 65 70 75 80

Ala Gly Val Gly Val lie Leu Ser Ala His Thr Ser Leu Gly Thr Trp 85 90 95 92

Pro lie Met Glu Phe Gly Thr Lys Glu Gin Lys Glu Lys Tyr Leu Val 100 105 110

Pro Leu Ala Ser Gly Lys Lys lie Ala Ala Phe Gly Leu Thr Glu Pro 115 120 125

Asn Ala Gly Ser Asp Ala Gly Lys Thr Glu Thr Thr Ala Val Leu Glu 130 135 140

Gly Asp His Tyr Val lie Asn Gly Ser Lys Val Phe lie Thr Asn Ala 145 150 155 160

Asp Tyr Ala Asp Thr Tyr Val lie Phe Ala Val Thr Thr Pro Gly Leu 165 170 175

Gly Thr Lys Gly lie Ser Ala Phe lie lie Glu Lys Gly Met Asp Gly 180 185 190

Phe Thr Phe Gly Thr His Tyr Asn Lys Met Gly lie Arg Ser Ser Ala 195 200 205

Thr Ala Glu Leu Leu Phe Lys Asn Leu Lys Val Pro Lys Tyr Asn Leu 210 215 220

Leu Gly Lys Glu Asn Glu Gly Phe Lys lie Ala Met Gin Thr Leu Glu 225 230 235 240

Gly Gly Arg lie Gly lie Ala Ala Gin Ala Leu Gly lie Ala Gin Gly 245 250 255

Ala Tyr Glu Lys Ala Leu Ser Tyr Ser Lys Glu Arg Val Gin Phe Gly 260 265 270

Lys Pro lie Ser Arg Gin Gin Ser lie Ala Phe Lys Leu Ala Asp Met 275 280 285

Ala Thr Lys lie Arg Ala Ala Arg Phe Met Val Tyr Ser Ala Ala Val 290 295 300

Leu Lys Gin Glu His Lys Asn Tyr Gly Met Glu Ser Ala Met Ala Lys 305 310 315 320

Leu Tyr Ala Ser Asp lie Cys Leu Glu Val Val Asn Asp Ala Val Gin 325 330 335 lie Tyr Gly Gly Ser Gly Phe lie Lys Gly Phe Pro Val Glu Arg Met 340 345 350

Tyr Arg Asp Ala Lys lie Cys Thr lie Tyr Glu Gly Thr Asn Glu lie 355 360 365

Gin Arg Leu lie lie Ser Asn Asp lie Leu Gly Lys Pro Lys Lys Glu 370 375 380

Pro lie Glu Glu Asn Lys Glu Asn Lys Val Asn Lys Ala Lys Pro lie 385 390 395 400 93

Thr Gly Asn Arg Arg Arg Val lie lie Lys Glu Gly Ser Pro Lys Glu 405 410 415

Lys Val Asp Ala Phe Leu Asn Tyr lie Lys Ser Glu Asn lie Asp lie 420 425 430

Asn Lys Ser Glu Ala Ser Lys Gly Ser lie Ala Asp Ala Asp Lys Val 435 440 445

Cys Ser lie Gly Leu Gly Leu Lys Asp Lys Lys Asp Leu Pro Leu lie 450 455 460

Gin Ser Leu Ala Asp Thr Val Gly Ala Glu Leu Gly Cys Ser Arg Pro 465 470 475 480

Val Ala Glu Glu Arg Glu Trp Leu Pro Leu Asp Arg Tyr Val Gly lie 485 490 495

Ser Gly Gin Lys Phe Gly Gly Thr Phe Tyr Leu Ala lie Gly lie Ser 500

Gly Gin Val Gin His 515

Ala lie Asn lie Asp 530 505 510 Leu Lys Gly lie Glu Asn Ala Gly lie lie Thr 520 525 Glu Asp Ala Pro lie Phe Lys Ser Ser Asp Tyr 535 540

Gly lie Val Gly Asp Leu Tyr Glu lie Val Pro Leu Leu lie Glu Ala 545 550 555 560

Leu Lys

<210> 63 <211> 539 <212> PRT <213> Euglena gracilis <400> 63

Met Ser Cys Pro Ala Ser Pro Ser Ala Ala Val Val Ser Ala Gly Ala 15 10 15

Leu Cys Leu Cys Val Ala Thr Val Leu Leu Ala Thr Gly Ser Asn Pro 20 25 30

Thr Ala Leu Ser Thr Ala Ser Thr Arg Ser Pro Thr Ser Leu Val Arg 35 40 45

Gly Val Asp Arg Gly Leu Met Arg Pro Thr Thr Ala Ala Ala Leu Thr 50 55 60

Thr Met Arg Glu Val Pro Gin Met Ala Glu Gly Phe Ser Gly Glu Ala 65 70 75 80

Thr Ser Ala Trp Ala Ala Ala Gly Pro Gin Trp Ala Ala Pro Leu Val 85 90 95 94

Ala Ala Ala Ser Ser Ala Leu Ala Leu Trp Trp Trp Ala Ala Arg Arg 100 105 110

Ser Val Arg Arg Pro Leu Ala Ala Leu Ala Glu Leu Pro Thr Ala Val

Thr His 130 115 120 125 Leu Ala Pro Pro Met Ala Met Phe Thr Thr Thr Ala Lys Val 135 140 lie Gin 145 Pro Lys lie Arg Gly Phe lie Cys Thr Thr Thr His Pro lie 150 155 160 Gly Cys Glu Lys Arg Val Gin Glu Glu lie Ala Tyr Ala Arg Ala His 165 170 175 Pro Pro Thr Ser Pro Gly Pro Lys Arg Val Leu Val lie Gly Cys Ser 180 185 190 Thr Gly Tyr Gly Leu Ser Thr Arg lie Thr Ala Ala Phe Gly Tyr Gin 195 200 205 Ala Ala 210 Thr Leu Gly Val Phe Leu Ala Gly Pro Pro Thr Lys Gly Arg 215 220 Pro Ala 225 Ala Ala Gly Trp Tyr Asn Thr Val Ala Phe Glu Lys Ala Ala 230 235 240 Leu Glu Ala Gly Leu Tyr Ala Arg Ser Leu Asn Gly Asp Ala Phe Asp 245 250 255 Ser Thr Thr Lys Ala Arg Thr Val Glu Ala lie Lys Arg Asp Leu Gly 260 265 270 Thr Val Asp Leu Val Val Tyr Ser lie Ala Ala Pro Lys Arg Thr Asp 275 280 285 Pro Ala 290 Thr Gly Val Leu His Lys Ala Cys Leu Lys Pro lie Gly Ala 295 300 Thr Tyr 305 Thr Asn Arg Thr Val Asn Thr Asp Lys Ala Glu Val Thr Asp 310 315 320

Val Ser lie Glu Pro Ala Ser Pro Glu Glu lie Ala Asp Thr Val Lys

Val Met 325 330 335 Gly Gly Glu Asp Trp Glu Leu Trp lie Gin Ala Leu Ser Glu 340 345 350

Ala Gly Val Leu Ala Glu Gly Ala Lys Thr Val Ala Tyr Ser Tyr lie 355 360 365

Gly Pro Glu Met Thr Trp Pro Val Tyr Trp Ser Gly Thr lie Gly Glu 370 375 380

Ala Lys Lys Asp Val Glu Lys Ala Ala Lys Arg lie Thr Gin Gin Tyr 95 385 390 395 400

Gly Cys Pro Ala Tyr Pro Val Val Ala Lys Ala Leu Val Thr Gin Ala 405 410 415

Ser Ser Ala lie Pro Val Val Pro Leu Tyr lie Cys Leu Leu Tyr Arg 420 425 430

Val Met Lys Glu Lys Gly Thr His Glu Gly Cys lie Glu Gin Met Val 435 440 445

Arg Leu Leu Thr Thr Lys Leu Tyr Pro Glu Asn Gly Ala Pro lie Val 450 455 460

Asp Glu Ala Gly Arg Val Arg Val Asp Asp Trp Glu Met Ala Glu Asp 465 470 475 480

Val Gin Gin Ala Val Lys Asp Leu Trp Ser Gin Val Ser Thr Ala Asn 485 490 495

Leu Lys Asp lie Ser Asp Phe Ala Gly Tyr Gin Thr Glu Phe Leu Arg 500 505 510

Leu Phe Gly Phe Gly lie Asp Gly Val Asp Tyr Asp Gin Pro Val Asp 515 520 525

Val Glu Ala Asp Leu Pro Ser Ala Ala Gin Gin 530 535 <210> 64 <211> 303 <212> PRT <213> Homo sapiens <400>

Met Ala Ser Trp Ala Lys Gly Arg Ser Tyr Leu Ala Pro Gly Leu Leu 15 10 15

Gin Gly Gin Val Ala lie Val Thr Gly Gly Ala Thr Gly lie Gly Lys 20 25 30

Ala lie Val Lys Glu Leu Leu Glu Leu Gly Ser Asn Val Val lie Ala 35 40 45

Ser Arg Lys Leu Glu Arg Leu Lys Ser Ala Ala Asp Glu Leu Gin Ala 50 55 60

Asn Leu Pro Pro Thr Lys Gin Ala Arg Val lie Pro lie Gin Cys Asn 65 70 75 80

Lys Ser Thr Leu Asp 95 lie Arg Asn Glu Glu Glu Val Asn Asn Leu Val 85 90

Thr Phe Gly Lys lie Asn Phe Leu Val Asn Asn Gly Gly Gly Gin Phe 100 105 110

Leu Ser Pro Ala Glu His lie Ser Ser Lys Gly Trp His Ala Val Leu 96 115 120 125

Glu Thr Asn Leu Thr Gly Thr Phe Tyr Met Cys Lys Ala Val Tyr Ser 130 135 140

Ser Trp Met Lys Glu His Gly Gly Ser lie Val Asn lie lie Val Pro 145 150 155 160

Thr Lys Ala Gly Phe Pro Leu Ala Val His Ser Gly Ala Ala Arg Ala 165 170 175

Gly Val Tyr Asn Leu Thr Lys Ser Leu Ala Leu Glu Trp Ala Cys Ser 180 185 190

Gly lie Arg lie Asn Cys Val Ala Pro Gly Val lie Tyr Ser Gin Thr 195 200 205

Ala Val Glu Asn Tyr Gly Ser Trp Gly Gin Ser Phe Phe Glu Gly Ser 210 215 220

Phe Gin Lys lie Pro Ala Lys Arg lie Gly Val Pro Glu Glu Val Ser 225 230 235 240

Ser Val Val Cys Phe Leu Leu Ser Pro Ala Ala Ser Phe lie Thr Gly 245 250 255

Gin Ser Val Asp Val Asp Gly Gly Arg Ser Leu Tyr Thr His Ser Tyr 260 265 270

Glu Val Pro Asp His Asp Asn Trp Pro Lys Gly Ala Gly Asp Leu Ser 275 280 285

Val Val Lys Lys Met Lys Glu Thr Phe Lys Glu Lys Ala Lys Leu 290 295 300 <210> 65 <211> 303

<212> PRT <213> Mus musculus <400> 65

Met Gly Ser Trp Lys Thr Gly Gin Ser Tyr Leu Ala Ala Gly Leu Leu 15 10 15

Lys Asn Gin Val Ala Val Val Thr Gly Gly Gly Thr Gly lie Gly Lys 20 25 30

Ala Val Ser Arg Glu Leu Leu His Leu Gly Cys Asn Val Val lie Ala 35 40 45

Ser Arg Lys Leu Asp Arg Leu Thr Ala Ala Val Asp Glu Leu Arg Ala 50 55 60

Ser Leu Pro Pro Ser Ser Ser Ala Glu Val Ser Ala lie Gin Cys Asn 65 70 75 80 lie Arg Lys Glu Glu Glu Val Ser Asn Leu Val Lys Ser Thr Leu Ala 97 85 90 95

Lys Tyr Gly Lys lie 100 Asn Phe Leu Val Asn Asn Gly Gly Gin Gin Phe 105 110 Met Ala Pro Val Glu 115 Asp lie Thr Ala Lys Gly Trp His Ala Val lie 120 125 Glu Thr Asn Leu Thr 130 Gly Thr Phe Tyr Met Cys Lys Glu Val Tyr Asn 135 140 Ser Trp Met Arg Glu 145 His Gly Gly Ser lie Val Asn lie lie Val Leu 150 155 160 Leu Asn Asn Gly Phe 165 Pro Thr Ala Ala His Thr Gly Ala Ala Arg Glu 170 175 Gly Val Tyr Asn Leu 180 Thr Lys Ser Met Ala Leu Ala Trp Ala Ser Ser 185 190 Gly Val Arg lie Asn 195 Cys Val Ala Pro Gly Thr lie Tyr Ser Gin Thr 200 205 Ala Val Asp Asn Tyr 210 Gly Glu Met Gly Gin Thr Leu Phe Glu Met Ala 215 220 Phe Asp Ser lie Pro 225 Val Ser Ala Leu Gly Val Pro Glu Glu lie Ser 230 235 240 Leu Leu Ala Arq Phe 245 Leu Leu Ser Pro Ala Ala Ser Tyr lie Thr Gly 250 255 Gin Leu lie Asn Val 260 Asp Gly Gly Gin Ala Leu His Thr His Ala Phe 265 270 Ser lie Pro Asp His 275 Asp Asn Trp Pro Val Gly Ala Gly Asp Leu Ser 280 285 lie Val Lys Arg lie 290 <210> 66 <211> 373 Lys Glu Ser P He Lys Lys Lys Ala Lys Leu 295 300

<212> PRT <213 > Rattus norvegicus <400> 66

Met Leu Val Ser Arg Arg Leu Thr Gly Ala Arg Ala Arg Ala Pro Leu 15 10 15

Leu Ala Ser Leu Leu Glu Ala Trp Cys Arg Gin Gly Arg Thr Thr Ser 20 25 30 Ser Tyr Ser Ala Phe 35 Ser Glu Pro Ser His Val Arg Ala Leu Val Tyr 40 45

Gly Asn His Gly Asp Pro Ala Lys Val lie Gin Leu Lys Asn Leu Glu 98 50 55 60

Leu Thr Ala Val Glu Gly Ser Asp Val His Val Lys Met Leu Ala Ala 65 70 75 80

Pro lie Asn Pro Ser Asp lie Asn Met lie Gin Gly Asn Tyr Gly Leu 85 90 95

Leu Pro Lys Leu Pro Ala Val Gly Gly Asn Glu Gly Val Gly Gin Val 100 105 110 lie Ala Val Gly Ser Ser Val Ser Gly Leu Lys Pro Gly Asp Trp Val 115 120 125 lie Pro Ala Asn Ala Gly Leu Gly Thr Trp Arg Thr Glu Ala Val Phe 130 135 140

Ser Glu Glu Ala Leu lie Gly Val Pro Lys Asp lie Pro Leu Gin Ser 145 150 155 160

Ala Ala Thr Leu Gly Val Asn Pro Cys Thr Ala Tyr Arg Met Leu Val 165 170 175

Asp Phe Glu Gin Leu Gin Pro Gly Asp Ser Val lie Gin Asn Ala Ser 180 185 190

Asn Ser Gly Val Gly Gin Ala Val lie Gin He Ala Ser Ala Leu Gly 195 200 205

Leu Lys Thr lie Asn Val lie Arg Asp Arg Pro Asp lie Lys Lys Leu 210 215 220

Thr Asp Arg Leu Lys Asp Leu Gly Ala Asp Tyr Val Leu Thr Glu Glu 225 230 235 240

Glu Leu Arg Met Pro Glu Thr Lys Asn lie Phe Lys Asp Leu Pro Leu 245 250 255

Pro Arg Leu Ala Leu Asn Cys Val Gly Gly Lys Ser Ser Thr Glu Leu 260 265 270

Leu Arg His Leu Ala Pro Gly Gly Thr Met Val Thr Tyr Gly Gly Met 275 280 285

Ala Lys Gin Pro Val Thr Ala Ser Val Ser Met Leu lie Phe Lys Asp 290 295 300

Leu Lys Leu Arg Gly Phe Trp Leu Ser Gin Trp Lys Lys Asn His Ser 305 310 315 320

Pro Asp Glu Phe Lys Glu Leu lie Leu lie Leu Cys Asn Leu lie Arg 325 330 335

Gin Gly Gin Leu Thr Ala Pro Ala Trp Ser Gly lie Pro Leu Gin Asp 340 345 350 99

Tyr Gin Gin Ala Leu 355 Gin lie Leu Thr Met 370 Glu Ala Ser Met Lys Pro Phe Val Ser Leu Lys 360 365 <210> 67 <211> 380

<212> PRT <213> Saccharomyces cerevisiae <400> 67

Met Leu Pro Thr Phe 1 5 Lys Arg Tyr Met Ser Ser Ser Ala His Gin lie 10 15 Pro Lys His Phe Lys 20 Ser Leu lie Tyr Ser Thr His Glu Val Glu Asp 25 30 Cys Thr Lys Val Leu 35 Ser Val Lys Asn Tyr Thr Pro Lys Gin Asp Leu 40 45 Ser Gin Ser lie Val 50 Leu Lys Thr Leu Ala Phe Pro lie Asn Pro Ser 55 60 Asp lie Asn Gin Leu 65 Gin Gly Val Tyr Pro Ser Arg Pro Glu Lys Thr 70 75 80 Tyr Asp Tyr Ser Thr 85 Asp Glu Pro Ala Ala lie Ala Gly Asn Glu Gly 90 95 Val Phe Glu Val Val 100 Ser Leu Pro Ser Gly Ser Ser Lys Gly Asp Leu 105 110 Lys Leu Gly Asp Arg 115 Val lie Pro Leu Gin Ala Asn Gin Gly Thr Trp 120 125 Ser Asn Tyr Arg Val 130 Phe Ser Ser Ser Ser Asp Leu lie Lys Val Asn 135 140 Asp Leu Asp Leu Phe 145 Ser Ala Ala Thr Val Ser Val Asn Gly Cys Thr 150 155 160 Gly Phe Gin Leu Val 165 Ser Asp Tyr lie Asp Trp Asn Ser Asn Gly Asn 170 175 Glu Trp lie lie Gin 180 Asn Ala Gly Thr Ser Ser Val Ser Lys lie Val 185 190 Thr Gin Val Ala Lys 195 Ala Lys Gly lie Lys Thr Leu Ser Val lie Arg 200 205 Asp Arg A Sp Asn Phe 210 Asp Glu Val Ala Lys Val Leu Glu Asp Lys Tyr 215 220 Gly Ala Thr Lys Val 225 lie Ser Glu Ser Gin Asn Asn Asp Lys Thr Phe 230 235 240 100 201033370

Ala Lys Glu Val Leu Ser Lys lie Leu Gly Glu Asn Ala Arg Val Arg 245 250 255

Leu Ala Leu Asn Ser Val Gly Gly Lys Ser Ser Ala Ser lie Ala Arg 260 265 270

Lys Leu Glu Asn Asn Ala Leu Met Leu Thr Tyr Gly Gly Met Ser Lys 275 280 285

Gin Pro Val Thr Leu Pro Thr Ser Leu His lie Phe Lys Gly Leu Thr 290 295 300

Ser Lys Gly Tyr Trp Val Thr Glu Lys Asn Lys Lys Asn Pro Gin Ser 305 310 315 320

Lys lie Asp Thr lie Ser Asp Phe lie Lys Met Tyr Asn Tyr Gly His 325 330 335

Lie lie Ser Pro Arg Asp Glu lie Glu Thr Leu Thr Trp Asn Thr Asn 340 345 350

Thr Thr Thr Asp Glu Gin Leu Leu Glu Leu Val Lys Lys Gly lie Thr 355 360 365

Gly Lys Gly Lys Lys Lys Met Val Val Leu Glu Trp 370 375 380 <210> 68 <211> 365

<212> PRT <213> Penicillium Chrysogenum <400> 68

Met Ser Lys Arg Val Thr lie Phe Asp Pro Pro Glu Gin Asp His Ser 15 10 15

Lys Ala Leu lie Glu Asn Val Leu Asp Leu Thr Pro Val Val Asp Leu 20 25 30

Gly Pro Asp Val Phe Thr Asn Thr Arg Pro Leu Trp His Pro Pro Gly 35 40 45

Ala Arg Gly lie Tyr Gly Gly Ala Ala lie Ala Gin Ser Leu Ser Ala 50 55 60

Ala Met Arg Thr Val Pro Ala Asp Tyr Ala Val His Ser Met His Cys 65 70 75 80

Tyr Phe Val Leu Ala Gly Asp Ser Glu lie Pro lie Leu Tyr His Val 85 90 95

Glu Arg Val Arg Asp Gly Arg Ser Phe Val Thr Arg Thr Val Gin Ala 100 105 110

Arg Gin Arg Gly Arg Pro lie Phe Thr Thr Thr Leu Ser Phe Ser Arg 115 120 125 101 201033370

Val Gly Ser Gly Gly Glu Lys Thr Leu His His Ala Val Ser Lys 130 135 140

Asp Val Pro Leu Pro Glu Glu Ala Glu Pro Gly Ser Leu Lys Ala 145 150 155

Ser Asn Ala Gly Gly Gly Pro Phe Glu Ser Arg Lys Ala Gly lie 165 170 175

Asn Arg Thr Ser Pro Asn Pro Glu Asp Lys Lys Val Arg Arg Tyr 180 185 190

Arg Ala Arg Gly Ala lie Ser Glu Glu Gly Gly Tyr Gin Ala His 195 200 205

Ser Ala Leu Ala Tyr lie Thr Asp Ser Tyr Phe lie Gly Thr Val 210 215 220

Lie Val His Asp Val Pro Arg Phe Ser Ser Pro Ala Glu Leu Glu 225 230 235

Leu Leu Asn Ala Leu Lys Asn Pro Ser Asp Leu Asp Asp Glu Asp 245 250 255

Thr Arg Ala Leu Arg Glu Leu Lys Glu Glu Glu Ala Ala Glu Leu 260 265 270

Arg Arg Leu Glu Gly Ala Leu Asn Arg Ala Thr Gly Pro Lys Glu 275 280 285

Asp His Lys Glu Val Gly Met Met Val Ser Leu Asp His Ser lie 290 295 300

Phe His Asn Pro Arg Ala Phe Arg Ala Asp Glu Trp Met Leu Ser 305 310 315

He Glu Ser Pro Trp Ala Gly Glu Gly Arg Gly Leu Ala lie Gin 325 330 335

Leu Trp Ser Lys Asp Gly Val Leu lie Ala Thr Cys Thr Gin Glu 340 345 350

Pro Leu 160 Leu lie Leu Ser Lys 240 lie Arg Val Tyr Glu 320 Lys Val

Cys lie Cys Phe Tyr Ser Leu Tyr Ser Gly Gly Gin Cys 355 360 365 <210> 69 <211> 525 <212> PRT <213> Penicillium Chrysogenum <400> 69 Met Ser Ala Ser Ala Leu Leu Lys Ser Arg Val Arg Arg Pro Ser 15 10 15

Leu Lys Lys Leu Ala Lys Pro Glu Asp Leu Leu His His Phe Pro 20 25 30

Tyr Asn 102 201033370

Gly Thr Tyr lie Gly Trp Ser Gly Phe Thr Gly Val Gly Tyr Pro Lys 35 40 45

Met Thr Pro lie Ala Leu Ala Asp His Val Glu Lys Asn Asn Leu Gin 50 55 60

Gly Gin Leu Lys Tyr Asn Leu Phe Val Gly Ala Ser Ser Gly Ala Glu 65 70 75 80

Thr Glu Asn Arg Trp Ala Arg Leu Asn Met lie Glu Arg Arg Ser Pro 85 90 95

His Gin Val Gly Lys Glu lie Ala Lys Gly He Asn Asn Gly Gin lie 100 105 110

Lys Phe Phe Asp Lys His Leu Ser Met Phe Pro Ser Asp Leu Val Tyr 115 120 125

Gly Tyr Tyr Thr Leu Asn Lys Ser Lys Pro Thr lie Asp Val Ala Val 130 135 140 lie Glu Ala Ser Ala lie Thr Glu Asn Gly Gly lie lie Pro Gly Ala 145 150 155 160

Ser Val Gly Ala Ser Pro Glu Leu lie Gin Met Ala Asp Lys Val lie 165 170 175 lie Glu Val Asn Thr Ser Met Pro Ser Phe Glu Gly Leu His Asp lie 180 185 190

Thr Cys Ser Asp Leu Pro Pro Gly Arg Lys Pro Tyr Leu lie Met Ala 195 200 205

Pro Glu Asp Arg lie Gly Thr Ser Tyr lie Pro lie Asp Pro Glu Lys 210 215 220

Val Val Ala lie Val Glu Ser Asn Tyr Pro Asp Gin Thr Leu Pro Asn 225 230 235 240

Ala Pro Glu Asp Glu Gly Ser Gin Ala lie Ala Ser Asn Leu lie Glu 245 250 255

Phe Leu Lys His Glu Val Asn His Gly Arg Leu Pro Pro Asn Leu Leu 260 265 270

Pro lie Gin Ser Gly lie Gly Asn lie Ala Asn Ala Val Val Gly Gly 275 280 285

Leu Ser Lys Gly Gly Ala Asp Phe Lys Asn Leu Lys Val Trp Thr Glu 290 295 300

Val Leu Gin Asp Ser Phe Leu Asp Leu Phe Asp Ser Gly Asn Leu Asp 305 310 315 320

Phe Ala Thr Ala Thr Ser lie Arg Phe Ser Pro Asp Gly Phe Lys Arg 325 330 335 103

Phe Tyr Asp Asn Trp Glu Gin Tyr 340

Ala Gly Lys Leu Leu Leu Arg Ser 345 350

Gin Gin Val Ser Asn Ser Pro Glu 355 360 lie lie Arg Arg Leu Gly Cys lie 365

Gly Met Asn Thr Pro Val Glu Val 370 375

Asp lie Tyr Ala His Ala Asn Ser 380

Thr Cys Val Met Gly Ser Arg Met 385 390

Leu Asn Gly Leu Gly Gly Ser Ala 395 400

Asp Phe Leu Arg Asn Ser Lys Tyr Ser lie Met His Thr Pro Ser Ala 405 410 415

Arg Pro Thr Lys Thr Asp Pro Thr Gly Val Ser Cys lie Val Pro Phe 420 425 430

Ala Thr His lie Asp Gin Thr Glu His Asp Leu Asp Val Val Val Thr 435 440 445

Glu Gin Gly Leu Ala Asp Val Arg Gly Leu Ser Pro Arg Glu Arg Ala 450 455 460

Arg Val lie He Lys Asn Cys Ser His Pro Asp Tyr Thr Pro lie Leu 465 470 475 480

Thr Asp Tyr Leu Asp Arg Ala Glu Phe Glu Cys Leu Arg Lys Gly Met 485 490 495

Gly His Glu Pro His Met Leu Phe Gin Ala Phe Asn Met His Lys Asn 500 505 510

Leu Gin Glu Lys Gly Thr Met Lys lie Asp Ser Trp Glu 515 520 525

<210> 70 <211> 253 <212> PRT <213> Penicillium Chrysogenum <400> 70

Met 1 Asp Lys Tyr Thr 5 lie Pro Leu Glu Lys 10 Ala Val Glu Leu Thr 15 Pro lie Pro Ser Glu 20 Pro Asp Thr Phe Thr 25 Asn Ala Gin Pro Leu 30 Trp Ser

Phe Ala Gly Gly Tyr Gly Val Tyr Gly Gly Ser Thr Val Ala His Cys 35 40 45

Leu Val Ala Ala Gin Lys Thr Val Pro Cys Asp Tyr Val Ala His Ser 50 55 60

Leu His Cys Gly Phe Val Ser Pro Gly Asn Pro Lys Arg Pro lie Glu 65 70 75 80 104 201033370

Tyr Arg Val Glu Arg Thr Arg Asp Gly Lys Ser Phe lie Thr Arg Thr 85 90 95

Val His Ala Thr Gin Lys Ala Gly Val lie Ser Glu Ala lie Val Asn 100 105 110

Phe Val Arg Val Gly Thr Tyr Ser Asn Asn Ala Ser His Asp Leu Val 115 120 125

Asn Thr Gin lie Arg Tyr Trp Val Arg Ala Lys Asp Pro lie Gin Gin 130 135 140

Arg Ser Pro Gin Ala Gin Leu Ala Ala Leu Ala Tyr Met Ser Asp Ala 145 150 155 160

Tyr Leu lie Gly Ala Ala Val Gin Val His Asp Val Ala Gly Gin Ala 165 170 175

Phe Gly Thr Lys Met Ala Met Ala Ala Ser Leu Asn His Thr lie Tyr 180 185 190

Phe His Asn Pro Glu Ala Val Arg Ala Asp Glu Trp Met Cys Ser Glu 195 200 205

Arg Glu Ser Pro Trp Ala Gly Asn Asp Arg Ala Leu Val Val Gin Arg 210 215 220

Val Trp Ser Pro Glu Gly lie Leu Val Ala Thr Cys Val Gin Glu Gly 225 230 235 240

Val Leu Arg Val Gin Val Pro Ser Glu Lys Val Lys Leu 245 250 <210> 71 <211> 462

<212> PRT

<213> Penicillium Chrysogenum <400> 71

Met Phe Gly Leu Ser Arg Arg Ser Ala Leu Ala Arg Ser Lys Ser Leu 15 10 15

Ala Ala Leu Pro Ala Arg Ala Ala Val Ala Ala Gin Pro Ser Cys lie 20 25 30

Asn Thr Gin Gly Phe His Ser Thr Pro Ser Gin Ala Ala Ser Arg Pro 35 40 45

Thr Trp Met Pro Met Arg Val Lys Thr Pro Trp lie Glu Ala Leu Thr 50 55 60

Gin lie Arg Glu Ala Gin Lys Ala Gly Pro Gin Glu Asn Ala Gly Gin 65 70 75 80

Ser Val Lys Arg Asp Leu Ser Pro Lys Lys Met Ser Asp Ser Tyr Tyr 85 90 95 105

Ser Ala lie Leu Pro Leu Ala Gin Asp Lys Trp Leu Leu Asp Ser Tyr 100 105 110

Leu Asn Ala Ser Gly His lie Arg Leu Gly Ser Leu Leu Met Asp Leu 115 120 125

Asp Ala Leu Ala Gly lie Val Ala Tyr Arg His Thr Gly Asp Gly Val 130 135 140

Ser Thr Val Thr Ala Ala Cys Asp 145 150

Arg lie Thr lie Glu Asn Pro Leu 155 160

Met Glu lie Cys Asp Leu Glu Leu 165

Ser Gly Gin Cys Thr Tyr Ala Thr 170 175

Gly Arg Ser Ser Met Glu lie Ser 180

Leu Gin Val Thr Lys Ala Arg Pro 185 190

Glu Gly Gin Glu Ala Lys Pro Glu 195 200

Asp lie Leu lie Thr Cys Ala Phe 205

Thr Met Val Ser Leu Asp Pro Ala 210 215

Thr Lys Ala Pro Val Pro Val Ala 220

Pro Leu He Val Glu Thr Glu Glu 225 230

Glu Lys Arg Leu Phe Gin Lys Gly 235 240

Glu Ala Asn Tyr Gin Ala Lys Lys 245

Ala Leu Arg Thr Arg Ser Leu Leu 250 255

Gin Lys Ser Pro Asp Asp Glu Glu 260

Ser Asn Leu lie His Ser Met Trp 265 270

Thr Lys Glu Met Ser Tyr Leu Asn 275 280

Pro Gin Asn Pro Ala Thr Arg Pro 285

Ala Asn Gin Val Phe Met Ser Asp 290 295

Thr Val Leu Lys Ser Ala Met lie 300

Met Gin Pro Gin Asp Arg Asn Arg His Asn Phe Met lie Phe Gly Gly 305 310 315 320

Phe Leu Leu Lys Gin Thr Phe Glu Leu Ala Phe Cys Cys Ala Ala Ser 325 330 335

Phe Ala His Ala Arg Pro Asn Phe Val Ala Leu Asp Pro Ser Thr Phe 340 345 350

Glu Asn Pro Val Pro Val Gly Ser Val Leu Tyr Leu Arg Ala Thr Val 355 360 365

Ser Tyr Thr Glu Pro Glu Glu Arg Glu Gly Asp Ser Thr Lys Tyr Thr 370 375 380

Lys Val Gin Val Arg Val Asp Ser Lys Val Arg Asp Val Glu His Gly 385 390 395 400 106 201033370

Thr Lys Lys Ser Thr Gly Met Phe Asn Tyr Thr Phe Leu Val Glu Lys 405 410 415

Asp Val Gin Val Met Pro Lys Gly Tyr Gly Glu Phe Met Leu Trp Ala 420 425 430

Asp Ala Arg Arg Arg Ala Gin Asn Ala Ala Ala lie Asp Pro Ala His 435 440 445

Lys Met Ser Ala Leu Arg Ser lie Lys Asp Ser Val Thr Glu 450 455 460 <210> 72 <211> 357

<212> PRT <213> Penicillium Chrysogenum <400> 72

Met Ser Pro Gin Arg Gly Ala Pro Asp Asn Asp Asp Leu Thr Ser Pro 15 10 15

His Ser Phe Val Glu Leu Met Ser Leu Gin Arg Leu Glu Asp Ala Leu 20 25 30 lie Thr Tyr Pro Asp Ser Ala Lys Pro Glu Lys lie Glu Arg Phe Arg 35 40 45

Ser Leu Ala Thr Pro Tyr Asn Pro Gly Gin Gly Thr Arg Ser Phe Gly 50 55 60

Gly His Val Tyr Ala Gin Ser Ala Tyr Ala Ala Ser Lys Thr Val Gly 65 70 75 80

Pro Gly Leu Val lie His Asp Met Thr Gly Thr Phe lie Leu Gly Gly 85 90 95

Leu Leu Asp Thr Pro Tyr Val Tyr Thr Val Arg His lie Arg Asp Gly 100 105 110

Tyr Met Tyr Ser Thr Arg Ala Val Asp Ala Arg Gin Ala Gly Arg lie 115 120 125

Cys Phe Ser Cys lie Cys Ser Phe Lys Arg Asp Glu Lys Gin Arg Leu 130 135 140

Phe Gin His Gin Pro Ala Ser Ala Gin Ala Arg Phe Asp Ser lie Leu 145 150 155 160

Ser Ala Lys Arg Pro Glu Asp Gin Glu Pro Ser Pro Ser Val Asp Ala 165 170 175

Glu Trp Trp lie Glu Ala Val Arg Gin Gly Asn lie Ser Glu Ser Glu 180 185 190

Phe Pro Gly Leu Asp Val Arg Lys Thr Asp Met Lys Asp Tyr Asn Cys 195 200 205 107

Ala Glu Asp Val Lys 210 Gin His Pro Glu Arg Tyr Arg Gin Leu Thr Gin 215 220 Tyr Arg Leu Lys Gly 225 Ser Pro Glu Glu Asp Pro Ala Ala Ser Leu Ala 230 235 240 Gin lie Arg Glu Arg 245 Glu Glu Asn Gly Glu Tyr Asp Asn Leu Tyr Ala 250 255 Cys Ala His Met Tyr 260 Ser Ser Asp Lys Asn Ser Leu Leu Leu lie Pro 265 270 Arg Ala Leu Gly lie 275 Lys Asn Trp Thr Glu Met Ala Ser Leu Thr Leu 280 285 Thr Val lie Val His 290 Gin His Gly Glu Ala Leu Arg Met Val Asn Trp 295 300 Asp Ser lie Gly Asp 305 Ser Asp Val Gly Val Asp Lys Leu Pro Met Lys 310 315 320 Trp Phe Val Gin Glu 325 Gly Trp Thr Pro Arg Ala Thr Glu Asn Arg Gly 330 335 Thr His Glu Ser His 340 Ser Leu Gin Asp Asn 355 Leu Trp Ser Pro Asp Gly Thr Leu Leu Ala Thr 345 350 <210> 73 <211> 320 <212> PRT <213> Mus musculus <400> 73 Met Ser Ala Pro Glu 1 5 Gly Leu Gly Asp Ala His Gly Asp Ala Asp Arg 10 15 Gly Asp Leu Ser Gly 20 Asp Leu Arg Ser Val Leu Val Thr Ser Val Leu 25 30 Asn Leu Glu Pro Leu 35 Asp Glu Asp Leu Tyr Arg Gly Arg His Tyr Trp 40 45 Val Pro Thr Ser Gin 50 Arg Leu Phe Gly Gly Gin lie Met Gly Gin Ala 55 60 Leu Val Ala Ala Ala 65 Lys Ser Val Ser Glu Asp Val His Val His Ser 70 IS 80 Leu His Cys Tyr Phe 85 Val Arg Ala Gly Asp Pro Lys Val Pro Val Leu 90 95 Tyr His Val Glu Arg 100 lie Arg Thr Gly Ala Ser Phe Ser Val Arg Ala 105 110 108

Val Lys Ala Val Gin His Gly Lys Ala lie Phe lie Cys Gin Ala Ser 115 120 125

Phe Gin Gin Met Gin Pro Ser Pro Leu Gin His Gin Phe Ser Met Pro 130 135 140

Ser Val Pro Pro Pro Glu Asp Leu Leu Asp His Glu Ala Leu lie Asp 145 150 155 160

Gin Tyr Leu Arg Asp Pro Asn Leu His Lys Lys Tyr Arg Val Gly Leu 165 170 175

Asn Arg Val Ala Ala Gin Glu Val Pro lie Glu lie Lys Val Val Asn 180 185 190

Pro Pro Thr Leu Thr Gin Leu Gin Ala Leu Glu Pro Lys Gin Met Phe 195 200 205

Trp Val Arg Ala Arg Gly Tyr lie Gly Glu Gly Asp lie Lys Met His 210 215 220

Cys Cys Val Ala Ala Tyr lie Ser Asp Tyr Ala Phe Leu Gly Thr Ala 225 230 235 240

Leu Leu Pro His Gin Ser Lys Tyr Lys Val Asn Phe Met Ala Ser Leu 245 250 255

Asp His Ser Met Trp Phe His Ala Pro Phe Arg Ala Asp His Trp Met 260 265 270

Leu Tyr Glu Cys Glu Ser Pro Trp Ala Gly Gly Phe Arg Gly Leu Val 275 280 285

His Gly Arg Leu Trp Arg Arg Asp Gly Val Leu Ala Val Thr Cys Ala 290 295 300

Gin Glu Gly Val lie Arg Leu Lys Pro Gin Val Ser Glu Ser Lys Leu 305 310 315 320 <210> 74 <211> 472

<212> PRT <213> Clostridium kluyveri <400> 74

Met Ser Asn Glu Val Ser lie Lys Glu Leu lie Glu Lys Ala Lys Ala 15 10 15

Ala Gin Lys Lys Leu Glu Ala Tyr Ser Gin Glu Gin Val Asp Val Leu 20 25 30

Val Lys Ala Leu Gly Lys Val Val Tyr Asp Asn Ala Glu Met Phe Ala 35 40 45

Lys Glu Ala Val Glu Glu Thr Glu Met Gly Val Tyr Glu Asp Lys Val 50 55 60 109

Ala Lys Cys 65 His Leu Lys Ser Gly Ala lie Trp Asn His lie Lys Asp 70 75 80 Lys Lys Thr Val Gly lie lie Lys Glu Glu Pro Glu Arg Ala Leu Val 85 90 95 Tyr Val Ala Lys Pro Lys Gly Val Val Ala Ala Thr Thr Pro lie Thr 100 105 110 Asn Pro Val 115 Val Thr Pro Met Cys Asn Ala Met Ala Ala lie Lys Gly 120 125 Arg Asn Thr 130 lie lie Val Ala Pro His Pro Lys Ala Lys Lys Val Ser 135 140 Ala His Thr 145 Val Glu Leu Met Asn Ala Glu Leu Lys Lys Leu Gly Ala 150 155 160 Pro Glu Asn lie lie Gin lie Val Glu Ala Pro Ser Arg Glu Ala Ala 165 170 175 Lys Glu Leu Met Glu Ser Ala Asp Val Val lie Ala Thr Gly Gly Ala 180 185 190 Gly Arg Val 195 Lys Ala Ala Tyr Ser Ser Gly Arg Pro Ala Tyr Gly Val 200 205 Gly Pro Gly 210 Asn Ser Gin Val lie Val Asp Lys Gly Tyr Asp Tyr Asn 215 220 Lys Ala Ala 225 Gin Asp lie lie Thr Gly Arg Lys Tyr Asp Asn Gly lie 230 235 240 lie Cys Ser Ser Glu Gin Ser Val lie Ala Pro Ala Glu Asp Tyr Asp 245 250 255 Lys Val lie Ala Ala Phe Val Glu Asn Gly Ala Phe Tyr Val Glu Asp 260 265 270 Glu Glu Thr Val Glu Lys Phe Arg Ser Thr Leu Phe Lys Asp Gly Lys 275 280 285 lie Asn Ser Lys lie lie Gly Lys Ser Val Gin lie lie Ala Asp Leu 290 295 300 Ala Gly Val 305 Lys Val Pro Glu Gly Thr Lys Val lie Val Leu Lys Gly 310 315 320

Lys Gly Ala Gly Glu Lys Asp Val Leu Cys Lys Glu Lys Met Cys Pro

Val Leu Val 325 330 335 Ala Leu Lys Tyr Asp Thr Phe Glu Glu Ala Val Glu lie 340 345 350

Ala Met Ala Asn Tyr Met Tyr Glu Gly Ala Gly His Thr Ala Gly lie 110 355 360 365

His Ser Asp Asn Asp Glu Asn lie Arg Tyr Ala Arq Thr Val Leu Pro 370 375 380 lie Ser Arg Leu Val Val Asn Gin Pro Ala Thr Thr Ala Gly Gly Thr 385 390 395 400

Val Leu Pro lie Ser Arg Leu Val Val Asn Gin Pro Ala Thr Thr Ala 405 410 415

Gly Gly Ser Phe Asn Asn Gly Phe Asn Pro Thr Thr Thr Leu Gly Cys 420 425 430

Gly Ser Trp Gly Arg Asn Ser lie Ser Glu Asn Leu Thr Tyr Glu His 435 440 445

Leu lie Asn Val Ser Arg lie Gly Tyr Phe Asn Lys Glu Ala Lys Val 450 455 460

Pro Ser Tyr Glu Glu lie Trp Gly 465 470 <210> 75 <211> 451

<212> PRT <213> Porphyromonas gingivalis <400> 75

Met Glu lie Lys Glu Met Val Ser Leu Ala Arg Lys Ala Gin Lys Glu 15 10 15

Tyr Gin Ala Thr His Asn Gin Glu Ala Val Asp Asn lie Cys Arg Ala 20 25 30

Ala Ala Lys Val lie Tyr Glu Asn Ala Ala lie Leu Ala Arg Glu Ala 35 40 45

Val Asp Glu Thr Gly Met Gly Val Tyr Glu His Lys Val Ala Lys Asn 50 55 60

Gin Gly Lys Ser Lys Gly Val Trp Tyr Asn Leu His Asn Lys Lys Ser 65 70 75 80 lie Gly lie Leu Asn lie Asp Glu Arg Thr Gly Met lie Glu lie Ala 85 90 95

Lys Pro lie Gly Val Val Gly Ala Val Thr Pro Thr Thr Asn Pro lie 100 105 110

Val Thr Pro Met Ser Asn lie lie Phe Ala Leu Lys Thr Cys Asn Ala 115 120 125 lie lie lie Ala Pro His Pro Arg Ser Lys Lys Cys Ser Ala His Ala 130 135 140

Val Arg Leu lie Lys Glu Ala lie Ala Pro Phe Asn Val Pro Glu Gly 111 145 150 155 160

Met Met Lys Asn Glu 225 Gly Thr Asp Val lie 305 Ala Leu Leu Gin Val 385 Gly Ser lie

Val Gin lie lie Glu Glu Pro Ser lie Glu Lys Thr Gin Glu Leu 165 170 175

Gly Ala Val Asp Val Val Val Ala Thr Gly Gly Met Gly Met Val 180 185 190

Ser Ala Tyr Ser Ser Gly Lys Pro Ser Phe Gly Val Gly Ala Gly 195 200 205

Val Gin Val lie Val Asp Ser Asn lie Asp Phe Glu Ala Ala Ala 210 215 220

Lys lie lie Thr Gly Arg Ala Phe Asp Asn Gly lie lie Cys Ser 230 235 240

Glu Gin Ser lie lie Tyr Asn Glu Ala Asp Lys Glu Ala Val Phe 245 250 255

Ala Phe Arg Asn His Gly Ala Tyr Phe Cys Asp Glu Ala Glu Gly 260 265 270

Arg Ala Arg Ala Ala lie Phe Glu Asn Gly Ala lie Ala Lys Asp 275 280 285

Val Gly Gin Ser Val Ala Phe lie Ala Lys Lys Ala Asn lie Asn 290 295 300

Pro Glu Gly Thr Arg lie Leu Val Val Glu Ala Arg Gly Val Gly 310 315 320

Glu Asp Val lie Cys Lys Glu Lys Met Cys Pro Val Met Cys Ala 325 330 335

Ser Tyr Lys His Phe Glu Glu Gly Val Glu lie Ala Arg Thr Asn 340 345 350

Ala Asn Glu Gly Asn Gly His Thr Cys Ala lie His Ser Asn Asn 355 360 365

Ala His lie lie Leu Ala Gly Ser Glu Leu Thr Val Ser Arg lie 370 375 380

Val Asn Ala Pro Ser Ala Thr Thr Ala Gly Gly His lie Gin Asn 390 395 400

Leu Ala Val Thr Asn Thr Leu Gly Cys Gly Ser Trp Gly Asn Asn 405 410 415 lie Ser Glu Asn Phe Thr Tyr Lys His Leu Leu Asn lie Ser Arg 420 425 430

Ala Pro Leu Asn Ser Ser lie His lie Pro Asp Asp Lys Glu lie 435 440 445 112 201033370

Trp Glu Leu 450 <210> 76 <211> 876

<212> PRT <213> Clostridium kluyveri <400> 76

Met Lys Val Thr Asn Val Glu Glu Leu Met Lys Lys Leu Gin Lys Val 15 10 15

Arg Gin Ala Gin Glu Lys Phe Ser Thr Tyr Thr Gin Glu Gin Val Asp 20 25 30

Glu lie Phe Lys Gin Ala Ala Met Ala Ala Asn Ser Ala Arg lie Lys 35 40 45

Leu Ala Lys Met Ala Val Glu Glu Thr Gly Met Gly lie Val Glu Asp 50 55 60

Lys Val lie Lys Asn His Phe Val Ser Glu Tyr Val Tyr Asn Lys Tyr 65 70 75 80

Lys Asp Glu Gin Thr Cys Gly Val Leu Glu Arg Asp Glu Gly Phe Gly 85 90 95

Met Val Arg lie Ala Glu Pro Val Gly Val lie Ala Ala Val Val Pro 100 105 110

Thr Thr Asn Pro Thr Ser Thr Ala lie Phe Lys Ala Leu lie Ala Leu 115 120 125

Lys Thr Arg Asn Gly lie Val Phe Ser Pro His Pro Arg Ala Lys Arg 130 135 140

Ser Thr Val Ala Ala Ala Lys lie Val Leu Asp Ala Ala Val Glu Ala 145 150 155 160

Gly Ala Pro Val Glu lie lie Gly Trp lie Asp Glu Pro Ser lie Glu 165 170 175

Leu Ser Gin Leu Val Met Lys Glu Ala Asp Leu lie Leu Ala Thr Gly 180 185 190

Gly Pro Gly Met Val Arg Ala Ala Tyr Ser Ser Gly Lys Pro Ala lie 195 200 205

Gly Val Gly Pro Gly Asn Thr Pro Val lie lie Asp Asp Ser Ala Asn 210 215 220 lie Lys Met Ala Val Asn Ser lie Leu Leu Ser Lys Ser Phe Asp His 225 230 235 240

Gly Val lie Cys Ala Ser Glu Gin Ser Val Met Val Leu Asp Arg lie 245 250 255 113

Tyr Glu Glu Val Lys Thr Glu Phe Ser Glu Arg Gly Gly Tyr lie Leu 260 265 270

Lys Asn Asp Glu lie Asp Lys Val Arg Glu Thr lie Leu Val Asn Gly 275 280 285 Ser lie Ser Pro Asp 290 lie Val Gly Gin Ser Ala Arg Lys lie Ala Glu 295 300

Met Ala Gly Val Lys Val Pro Glu Arg Thr Lys lie Leu lie Gly Glu 305 310 315 320 Val Glu Ser Val Glu 325 Leu Glu Glu Pro Phe Ser Arg Glu Lys Leu Ser 330 335 Pro Val Leu Ala Met 340 Tyr Arg Val Lys Asn Phe Asn Asp Gly Leu Lys 345 350 Lys Ala Ala Arg Leu 355 Val Glu Leu Gly Gly Gly His Thr Ser Val 360 365 Leu Tyr Val Asn Thr 370 Met Thr Gin Lys Glu Lys Val Glu Lys Phe Ser 375 380 Glu Ala Met Lys Thr 385 Gly Arg Thr Leu lie Asn Met Pro Ser Ala Gin 390 395 400 Gly Ala lie Gly Asp 405 Leu Tyr Asn Phe Lys Leu Ala Pro Ser Leu Thr 410 415 Leu Gly Cys Gly Ser 420 Trp Gly Gly Asn Ser lie Ser Glu Asn Val Gly 425 430 Pro Lys His Leu Leu 435 Asn lie Lys Ser lie Ala Glu Arg Arg Glu Asn 440 445 Met Leu Trp Phe Arg 450 Val Pro Glu Lys lie Tyr Phe Lys Tyr Gly Ala 455 460 Leu Gly Thr Ala Leu 465 Lys Glu Leu Lys Thr Met His Lys Lys Lys Val 470 475 480 Phe lie Val Thr Asp 485 Lys Val Leu Tyr Gin Leu Gly Tyr Thr Asp Ser 490 495 lie Thr Lys lie Leu Gly Glu Met Gly Val Gly Tyr Lys lie Phe Thr 5 00 505 510

Asp Val Glu Pro Asp Pro Thr Leu Ala Thr Ala Lys Lys Gly Ser Ala 515 520 525

Glu Met Leu Gin Tyr Asn Pro Asp Thr lie lie Ser Val Gly Gly Gly 530 535 540

Ser Ala Met Asp Ala Ala Lys lie Met Trp Val Met Tyr Glu His Pro 545 550 555 560 114

Glu lie Lys Phe Glu Asp Leu Ala 565

Met Arg Phe Met Asp lie Arg Lys 570 575

Arg Val Tyr Thr Phe Pro Lys Met 580

Gly Glu Lys Ser Met Met lie Ser 585 590

Val Ala Thr Ser Ala Gly Thr Gly 595 600

Ser Glu Val Thr Pro Phe Thr Val 605 lie Thr Asp Glu Lys Thr Gly Ala 610 615

Lys Tyr Pro Leu Ala Asp Tyr Glu 620

Leu Thr Pro Asp lie Ala lie lie 625 630

Asp Ala Glu Leu Met Met Asn Met 635 640

Pro Lys Gly Leu Thr Ala Ala Ser 645

Gly lie Asp Ala Met Thr His Ala 650 655

Leu Glu Ser Tyr Val Ser Val Met 660

Ala Ser Glu Tyr Thr Asn Gly Leu 665 670

Ala Leu Glu Ala lie Arg Leu lie 675 680

Phe Lys Tyr Leu Pro Leu Ala Tyr 685

Ala Glu Gly Thr Thr Asn Val Lys 690 695

Ala Arg Glu Lys Met Ala His Ala 700

Ser Thr Met Ala Gly Met Ala Phe 705 710

Ala Asn Ala Phe Leu Gly Val Cys 715 720

His Ser Met Ala His Lys Leu Gly 725

Ala Ala His His lie Pro His Gly 730 735 lie Ala Asn Ala Leu Met lie Asp 740

Glu Val lie Lys Phe Asn Ala Val 745 750

Asn Asp Pro Arg Lys Gin Ala lie 755 760

Phe Pro Gin Tyr Lys Tyr Pro Asn 765

Ala Lys Trp Arg Tyr Ala Arg lie 770 775

Ala Asp Tyr Leu Glu Leu Gly Gly 780

Asn Ser Glu Asp Glu Lys Val Gly 785 790

Leu Leu lie Lys Ala lie Asp Glu 795 800

Leu Lys Glu Lys lie Asn lie Pro 805

Lys Thr lie Arg Glu Ala Gly Val 810 815

Ala Glu Lys Lys Phe Tyr Ala Thr 820

Leu Asp Asp Met Ser Glu Gin Ala 825 830

Phe Asp Asp Gin Cys Thr Gly Ala 835 840

Asn Pro Arg Tyr Pro Leu lie Ser 845

Glu lie Lys Gin Met Tyr lie Asn 850 855

Ala Phe Asp Lys Val Ser Pro Val 860 115

Val Asp Glu Glu Glu Lys Leu Lys Val Ser Leu Leu 865 870 875 <210> 77 <211> 491

<212> PRT <213> Clostridium kluyveri <400> 77

Met Glu lie Met Asp Lys Asp Leu Gin Ser lie Gin Glu Val Arg Thr 15 10 15

Leu lie Ala Lys Ala Lys Lys Ala Gin Ala Glu Phe Lys Asn Phe Ser 20 25 30

Gin Glu Ala Val Asn Lys Val lie Glu Lys lie Ala Lys Ala Thr Glu 35 40 45

Val Glu Ala Val Lys Leu Ala Lys Leu Ala Tyr Glu Asp Thr Gly Tyr 50 55 60

Gly Lys Trp Glu Asp Lys Val lie Lys Asn Lys Phe Ser Ser lie Val 65 70 75 80

Val Tyr Asn Tyr lie Lys Asp Leu Lys Thr Val Gly lie Leu Lys Glu 85 90 95

Asp Lys Glu Lys Lys Leu lie Asp lie Ala Val Pro Leu Gly Val lie 100 105 110

Ala Gly Leu lie Pro Ser Thr Asn Pro Thr Ser Thr Ala lie Phe Lys 115 120 125

Val Leu lie Ala Leu Lys Ala Gly Asn Ala lie Val Phe Ser Pro His 130 135 140

Pro Thr Ala Val Arg Ser lie Thr Glu Thr Val Lys lie Met Gin Lys 145 150 155 160

Ala Ala Val Glu Ala Gly Ala Pro Asp Gly Leu lie Gin Cys Met Ser 165 170 175 lie Leu Thr Val Glu Gly Thr Ala Glu Leu Met Lys Asn Lys Asp Thr 180 185 190

Ala Leu lie Leu Ala Thr Gly Gly Glu Gly Met Val Arg Ala Ala Tyr 195 200 205

Ser Ser Gly Thr Pro Ala lie Gly Val Gly Pro Gly Asn Gly Pro Cys 210 215 220

Phe lie Glu Arg Thr Ala Asp lie Pro Thr Ala Val Arg Lys Val lie 225 230 235 240

Gly Ser Asp Thr Phe Asp Asn Gly Val lie Cys Ala Ser Glu Gin Ser 245 250 255 116 lie lie Ala Glu Thr Val Lys Lys Ala Glu lie lie Glu Glu Phe Lys 260 265 270

Arg Gin Lys Gly Tyr Phe Leu Asn Ala Glu Glu Ser Glu Lys Val Gly 275 280 285

Lys lie Leu Leu Arg Ala Asn Gly Thr Pro Asn Pro Ala lie Val Gly 290 295 300

Lys Asp Val Gin Ala Leu Ala Lys Leu Ala Gly lie Ser lie Pro Ser 305 310 315 320

Asp Ala Val lie Leu Leu Ser Glu Gin Thr Asp Val Ser Pro Lys Asn 325 330 335

Pro Tyr Ala Lys Glu Lys Leu Ala Pro Val Leu Ala Phe Tyr Thr Val 340 345 350

Glu Asp Trp His Glu Ala Cys Glu Lys Ser Leu Ala Leu Leu His Asn 355 360 365

Gin Gly Ser Gly His Thr Leu lie lie His Ser Gin Asn Glu Glu lie 370 375 380 lie Arg Glu Phe Ala Leu Lys Lys Pro Val Ser Arg lie Leu Val Asn 385 390 395 400

Ser Pro Gly Ser Leu Gly Gly lie Gly Gly Ala Thr Asn Leu Val Pro 405 410 415

Ser Leu Thr Leu Gly Cys Gly Ala Val Gly Gly Ser Ala Thr Ser Asp 420 425 430

Asn Val Gly Pro Glu Asn Leu Phe Asn lie Arg Lys Val Ala Tyr Gly 435 440 445

Thr Thr Thr Val Glu Glu He Arg Glu Ala Phe Gly Val Gly Ala Ala 450 455 460

Ser Ser Ser Ala Pro Ala Glu Pro Glu Asp Asn Glu Asp Val Gin Ala 465 470 475 480 lie Val Lys Ala lie Met Ala Lys Leu Asn Leu 485 490 <210> 78 <211>

<212> PRT <213> Clostridium kluyveri <400> 78

Met Glu lie Met Asp Lys Asp Leu Gin Ser lie Gin Glu Val Arg Thr 15 10 15

Leu lie Ala Lys Ala Lys Lys Ala Gin Ala Glu Phe Lys Asn Phe Ser 20 25 30 117

Gin Glu Ala Val Asn Lys Val lie 35 40 Glu Lys lie Ala Lys Ala Thr Glu 45 Val Glu Ala Val Lys Leu Ala Lys 50 55 Leu Ala Tyr Glu Asp Thr Gly Tyr 60 Gly Lys Trp Glu Asp Lys Val lie 65 70 Lys Asn Lys Phe Ser Ser He Val 75 80 Val Tyr Asn Tyr lie Lys Asp Leu 85 Lys Thr Val Gly lie Leu Lys Glu 90 95 Asp Lys Glu Lys Lys Leu lie Asp 100 lie Ala Val Pro Leu Gly Val lie 105 110 Ala Gly Leu lie Pro Ser Thr Asn 115 120 Pro Thr Ser Thr Ala lie Phe Lys 125 Val Leu lie Ala Leu Lys Ala Gly 130 135 Asn Ala lie Val Phe Ser Pro His 140 Pro Thr Ala Val Arg Ser lie Thr 145 150 Glu Thr Val Lys lie Met Gin Lys 155 160 Ala Ala Val Glu Ala Gly Ala Pro 165 Asp Gly Leu lie Gin Cys Met Ser 170 175 lie Leu Thr Val Glu Gly Thr Ala 180 Glu Leu Met Lys Asn Lys Asp Thr 185 190 Ala Leu lie Leu Ala Thr Gly Gly 195 200 Glu Gly Met Val Arg Ala Ala Tyr 205 Ser Ser Gly Thr Pro Ala lie Gly 210 215 Val Gly Pro Gly Asn Gly Pro Cys 220 Phe lie Glu Arg Thr Ala Asp lie 225 230 Pro Thr Ala Val Arg Lys Val lie 235 240

Gly Ser Asp Thr Phe Asp Asn Gly Val lie Cys Ala Ser Glu Gin Ser 245 250 255 lie lie Ala Glu Thr Val Lys Lys 260 Ala Glu lie lie Glu Glu Phe Lys 265 270 Arg Gin Lys Gly Tyr Phe Leu Asn 275 280 Ala Glu Glu Ser Glu Lys Val Gly 285 Lys lie Leu Leu Arg Ala Asn Gly 290 295 Thr Pro Asn Pro Ala lie Val Gly 300 Lys Asp Val Gin Ala Leu Ala Lys 305 310 Leu Ala Gly lie Ser lie Pro Ser 315 320 Asp Ala Val lie Leu Leu Ser Glu Gin Thr Asp Val Ser Pro Lys Asn 118 325 330 335

Pro Tyr Ala Lys Glu Lys Leu Ala Pro Val Leu Ala Phe Tyr Thr Val 340 345 350

Glu Asp Trp His Glu Ala Cys Glu Lys Ser Leu Ala Leu Leu His Asn 355 360 365

Gin Gly Ser Gly His Thr Leu lie lie His Ser Gin Asn Glu Glu lie 370 375 380 lie Arg Glu Phe Ala Leu Lys Lys Pro Val Ser Arg lie Leu Val Asn 385 390 395 400

Ser Pro Gly Ser Leu Gly Gly lie Gly Gly Ala Thr Asn Leu Val Pro 405 410 415

Ser Leu Thr Leu Gly Cys Gly Ala Val Gly Gly Ser Ala Thr Ser Asp 420 425 430

Asn Val Gly Pro Glu Asn Leu Phe Asn lie Arg Lys Val Ala Tyr Gly 435 440 445

Thr Thr Thr Val Glu Glu lie Arg Glu Ala Phe Gly Val Gly Ala Ala 450 455 460

Ser Ser Ser Ala Pro Ala Glu Pro Glu Asp Asn Glu Asp Val Gin Ala 465 470 475 480 lie Val Lys Ala lie Met Ala Lys Leu Asn Leu 485 490 <210> 79 <211> 469

<212> PRT <213> Propionibacterium freudenreichi <400> 79

Met Thr lie Ser Pro Glu Leu lie Gin Gin Val Val Arg Glu Thr Val 15 10 15

Arg Glu Val lie Ser Arg Gin Asp Ser Gly Thr Asp Ala Pro Ser Gly 20 25 30

Thr Asp Gly lie Phe Thr Asp Met Asn Ser Ala Val Asp Ala Ala Asp 35 40 45

Val Ala Trp Arg Gin Tyr Met Asp Cys Ser Leu Arg Asp Arg Asn Arg 50 55 60

Phe lie Gin Ala lie Arg Asp Val Ala Ser Glu Pro Asp Asn Leu Glu 65 70 75 80

Tyr Met Ala Thr Ala Thr Val Glu Glu Thr Gly Met Gly Asn Val Pro 85 90 95

His Lys lie Leu Lys Asn Arg Tyr Ala Ala Leu Tyr Thr Pro Gly Thr 119 100 105 110

Glu Asp lie lie Thr 115 Glu Ala Trp Ser Gly Asp Asp Gly Leu Thr Thr 120 125 Val Glu Phe Ser Pro 130 Phe Gly Val lie Gly Ala lie Thr Pro Thr Thr 135 140 Asn Pro Thr Glu Thr 145 Val lie Asn Asn Thr lie Gly Met Leu Ala Ala 150 155 160 Gly Asn Ala Val Val 165 Phe Ser Pro His Pro Arg Ala Lys Lys lie Thr 170 175 Leu Trp Leu Val Arg 180 Lys lie Asn Arg Ala Leu Ala Ala Ala Gly Ala 185 190 Pro Ala Asn Leu Val 195 Val Thr Val Glu Glu Pro Ser lie Asp Asn Thr 200 205 Asn Ala Met Met Ser 210 His Glu Lys Val Arg Met Leu Val Ala Thr Gly 215 220 Gly Pro Gly He Val 225 Lys Ala Val Leu Ser Ser Gly Lys Lys Ala Lie 230 235 240

Gly Ala Gly Ala Gly Asn Pro Pro Ala Val Val Asp Asp Thr Ala Asp 245 250 255 lie Ala Lys Ala Ala 260 Arg Asp lie Val Asp Gly Ala Ser Phe Asp Asn 265 270 Asn Leu Pro Cys Thr 275 Ala Glu Lys Glu Val Leu Ala Val Asp Ser lie 280 285 Ala Asp Leu Leu Lys 290 Phe Glu Met Leu Lys His Gly Cys Phe Glu Leu 295 300 Lys Asp Arg Ala Val 305 Met Asp Lys Leu Ala Ala Leu Val Thr Lys Gly 310 315 320 Gin His Ala Asn Ala 325 Ala Tyr Val Gly Lys Pro Ala Ala Gin Leu Ala 330 335 Ser Glu Val Gly Leu 340 Ser Ala Pro Lys Asp Thr Arg Leu Leu lie Cys 345 350 Glu Val Pro Phe Asp 355 His Pro Phe Val Gin Val Glu Leu Met Met Pro 360 365 lie Leu Pro lie Val 370 Arg Met Pro Asp Val Asp Thr Ala lie Asp Lys 375 380 Ala Val Glu Val Glu 385 His Gly Asn Arg His Thr Ala Val Met His Ser 390 395 400 120 201033370

Ser Asn Val Asn Ala Leu Thr Lys Met Gly Lys Leu lie Gin Thr Thr 405 410 415 lie Phe Val Lys Asn Gly Pro Ser Tyr Asn Gly lie Gly lie Asp Gly 420 425 430

Glu Gly Phe Pro Thr Phe Thr lie Ala Gly Pro Thr Gly Glu Gly Leu 435 440 445

Thr Ser Ala Arg Cys Phe Ala Arg Lys Arg Arg Cys Val Leu Lys Ser 450 455 460

Gly Leu Asn lie Arg 465 <210> 80 <211> 858

<212> PRT <213> Clostridium acetobutylicum <400> 80

Met Lys Val Thr Asn Gin Lys Glu Leu Lys Gin Lys Leu Asn Glu Leu 15 10 15

Arg Glu Ala Gin Lys Lys Phe Ala Thr Tyr Thr Gin Glu Gin Val Asp 20 25 30

Lys lie Phe Lys Gin Cys Ala lie Ala Ala Ala Lys Glu Arg lie Asn 35 40 45

Leu Ala Lys Leu Ala Val Glu Glu Thr Gly lie Gly Leu Val Glu Asp 50 55 60

Lys lie lie Lys Asn His Phe Ala Ala Glu Tyr lie Tyr Asn Lys Tyr 65 70 75 80 ❿

Lys Asn Glu Lys Thr Cys Gly lie lie Asp His Asp Asp Ser Leu Gly 85 90 95 lie Thr Lys Val Ala Glu Pro lie Gly lie Val Ala Ala lie Val Pro 100 105 110

Thr Thr Asn Pro Thr Ser Thr Ala lie Phe Lys Ser Leu lie Ser Leu 115 120 125

Lys Thr Arg Asn Ala lie Phe Phe Ser Pro His Pro Arg Ala Lys Lys 130 135 140

Ser Thr lie Ala Ala Ala Lys Leu lie Leu Asp Ala Ala Val Lys Ala 145 150 155 160

Gly Ala Pro Lys Asn lie lie Gly Trp lie Asp Glu Pro Ser lie Glu 165 170 175

Leu Ser Gin Asp Leu Met Ser Glu Ala Asp lie lie Leu Ala Thr Gly 180 185 190 121

Gly Pro Ser Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala lie 195 200 205

Gly Val Gly Ala Gly Asn Thr Pro Ala lie lie Asp Glu Ser Ala Asp 210 215 220 lie Asp Met Ala Val Ser Ser lie lie Leu Ser Lys Thr Tyr Asp Asn 225 230 235 240

Gly Val lie Cys Ala Ser Glu Gin Ser lie Leu Val Met Asn Ser lie 245 250 255

Tyr Glu Lys Val Lys Glu Glu Phe Val Lys Arg Gly Ser Tyr lie Leu 260 265 270

Asn Gin Asn Glu lie Ala Lys lie Lys Glu Thr Met Phe Lys Asn Gly 275 280 285

Ala lie Asn Ala Asp lie Val Gly Lys Ser Ala Tyr lie lie Ala Lys 290 295 300

Met Ala Gly lie Glu Val Pro Gin Thr Thr Lys lie Leu lie Gly Glu 305 310 315 320

Val Gin Ser Val Glu Lys Ser Glu Leu Phe Ser His Glu Lys Leu Ser 325 330 335

Pro Val Leu Ala Met Tyr Lys Val Lys Asp Phe Asp Glu Ala Leu Lys 340 345 350

Lys Ala Gin Arg Leu lie Glu Leu Gly Gly Ser Gly His Thr Ser Ser 355 360 365

Leu Tyr lie Asp Ser Gin Asn Asn Lys Asp Lys Val Lys Glu Phe Gly 370 375 380

Leu Ala Met Lys Thr Ser Arg Thr Phe lie Asn Met Pro Ser Ser Gin 385 390 395 400

Gly Ala Ser Gly Asp Leu Tyr Asn Phe Ala lie Ala Pro Ser Phe Thr 405 410 415

Leu Gly Cys Gly Thr Trp Gly Gly Asn Ser Val Ser Gin Asn Val Glu 420 425 430

Pro Lys His Leu Leu Asn lie Lys Ser Val Ala Glu Arg Arg Glu Asn 435 440 445

Met Leu Trp Phe Lys Val Pro Gin Lys lie Tyr Phe Lys Tyr Gly Cys 450 455 460

Leu Arg Phe Ala Leu Lys Glu Leu Lys Asp Met Asn Lys Lys Arg Ala 465 470 475 480

Phe lie Val Thr Asp Lys Asp Leu Phe Lys Leu Gly Tyr Val Asn Lys 485 490 495 122 lie Asp Glu Ser 545 Glu Arg lie lie Leu 625 Pro lie Ala Lys Ser 705 His lie Asp Ala Thr 785

Thr Lys Val Leu Asp Glu lie Asp lie Lys Tyr Ser lie Phe Thr 500 505 510 lie Lys Ser Asp Pro Thr lie Asp Ser Val Lys Lys Gly Ala Lys 515 520 525

Met Leu Asn Phe Glu Pro Asp Thr lie lie Ser lie Gly Gly Gly 530 535 540

Pro Met Asp Ala Ala Lys Val Met His Leu Leu Tyr Glu Tyr Pro 550 555 560

Ala Glu lie Glu Asn Leu Ala lie Asn Phe Met Asp lie Arg Lys 565 570 575 lie Cys Asn Phe Pro Lys Leu Gly Thr Lys Ala lie Ser Val Ala 580 585 590

Pro Thr Thr Ala Gly Thr Gly Ser Glu Ala Thr Pro Phe Ala Val 595 600 605

Thr Asn Asp Glu Thr Gly Met Lys Tyr Pro Leu Thr Ser Tyr Glu 610 615 620

Thr Pro Asn Met Ala lie lie Asp Thr Glu Leu Met Leu Asn Met 630 635 640

Arg Lys Leu Thr Ala Ala Thr Gly lie Asp Ala Leu Val His Ala 645 650 655

Glu Ala Tyr Val Ser Val Met Ala Thr Asp Tyr Thr Asp Glu Leu 660 665 670

Leu Arg Ala lie Lys Met lie Phe Lys Tyr Leu Pro Arg Ala Tyr 675 680 685

Asn Gly Thr Asn Asp lie Glu Ala Arg Glu Lys Met Ala His Ala 690 695 700

Asn lie Ala Gly Met Ala Phe Ala Asn Ala Phe Leu Gly Val Cys 710 715 720

Ser Met Ala His Lys Leu Gly Ala Met His His Val Pro His Gly 725 730 735

Ala Cys Ala Val Leu lie Glu Glu Val lie Lys Tyr Asn Ala Thr 740 745 750

Cys Pro Thr Lys Gin Thr Ala Phe Pro Gin Tyr Lys Ser Pro Asn 755 760 765

Lys Arg Lys Tyr Ala Glu lie Ala Glu Tyr Leu Asn Leu Lys Gly 770 775 780

Ser Asp Thr Glu Lys Val Thr Ala Leu lie Glu Ala lie Ser Lys 790 795 800 123

Leu Lys lie Asp Leu 805 Ser lie Pro Gin Asn lie Ser Ala Ala Gly lie 810 815 Asn Lys Lys Asp Phe 820 Tyr Asn Thr Leu Asp Lys Met Ser Glu Leu Ala 825 830 Phe Asp Asp Gin Cys 835 Thr Thr Ala Asn Pro Arg Tyr Pro Leu lie Ser 840 845 Glu Leu Lys Asp lie 850 Tyr lie Lys Ser Phe 855 <210> 81 <211> 862 <212> PRT <213> Clostridium acetobutylicum <400>

Met Lys Val Thr Thr 1 5 Val Lys Glu Leu Asp Glu Lys Leu Lys Val lie 10 15 Lys Glu Ala Gin Lys 20 Lys Phe Ser Cys Tyr Ser Gin Glu Met Val Asp 25 30 Glu lie Phe Arg Asn 35 Ala Ala Met Ala Ala Lie Asp Ala Arg lie Glu 40 45 Leu Ala Lys Ala Ala 50 Val Leu Glu Thr Gly Met Gly Leu Val Glu Asp 55 60 Lys Val lie Lys Asn 65 His Phe Ala Gly Glu Tyr lie Tyr Asn Lys Tyr 70 75 80 Lys Asp Glu Lys Thr 85 Cys Gly lie lie Glu Arg Asn Glu Pro Tyr Gly 90 95 lie Thr Lys lie Ala 100 Glu Pro lie Gly Val Val Ala Ala lie lie Pro 105 110 Val Thr Asn Pro Thr 115 Ser Thr Thr lie Phe Lys Ser Leu lie Ser Leu 120 125 Lys Thr Arg Asn Gly 130 lie Phe Phe Ser Pro His Pro Arg Ala Lys Lys 135 140 Ser Thr lie Leu Ala 145 Ala Lys Thr lie Leu Asp Ala Ala Val Lys Ser 150 155 160 Gly Ala Pro Glu Asn 165 lie Lie Gly Trp lie Asp Glu Pro Ser lie Glu 170 175 Leu Thr Gin Tyr Leu 180 Met Gin Lys Ala Asp lie Thr Leu Ala Thr Gly 185 190 Gly Pro Ser Leu Val 195 Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala lie 200 205 124

Gly Val Gly Pro Gly Asn Thr Pro Val lie lie Asp Glu Ser Ala His 210 215 220 lie Lys Met Ala Val Ser Ser lie lie Leu Ser Lys Thr Tyr Asp Asn 225 230 235 240

Gly Val lie Cys Ala Ser Glu Gin Ser Val lie Val Leu Lys Ser lie 245 250 255

Tyr Asn Lys Val Lys Asp Glu Phe Gin Glu Arg Gly Ala Tyr lie lie 260 265 270

Lys Lys Asn Glu Leu Asp Lys Val Arg Glu Val lie Phe Lys Asp Gly 275 280 285

Ser Val Asn Pro Lys lie Val Gly Gin Ser Ala Tyr Thr lie Ala Ala 290 295 300

Met Ala Gly lie Lys Val Pro Lys Thr Thr Arg lie Leu lie Gly Glu 305 310 315 320

Val Thr Ser Leu Gly Glu Glu Glu Pro Phe Ala His Glu Lys Leu Ser 325 330 335

Pro Val Leu Ala Met Tyr Glu Ala Asp Asn Phe Asp Asp Ala Leu Lys 340 345 350

Lys Ala Val Thr Leu lie Asn Leu Gly Gly Leu Gly His Thr Ser Gly 355 360 365 lie Tyr Ala Asp Glu lie Lys Ala Arg Asp Lys lie Asp Arg Phe Ser 370 375 380

Ser Ala Met Lys Thr Val Arg Thr Phe Val Asn lie Pro Thr Ser Gin 385 390 395 400

Gly Ala Ser Gly Asp Leu Tyr Asn Phe Arg lie Pro Pro Ser Phe Thr 405 410 415

Leu Gly Cys Gly Phe Trp Gly Gly Asn Ser Val Ser Glu Asn Val Gly 420 425 430

Pro Lys His Leu Leu Asn lie Lys Thr Val Ala Glu Arg Arg Glu Asn 435 440 445

Met Leu Trp Phe Arg Val Pro His Lys Val Tyr Phe Lys Phe Gly Cys 450 455 460

Leu Gin Phe Ala Leu Lys Asp Leu Lys Asp Leu Lys Lys Lys Arg Ala 465 470 475 480

Phe lie Val Thr Asp Ser Asp Pro Tyr Asn Leu Asn Tyr Val Asp Ser 485 490 495 lie lie Lys lie Leu Glu His Leu Asp lie Asp Phe Lys Val Phe Asn 125 500 505 510

Lys Val Gly Arg Glu Ala Asp Leu Lys Thr lie Lys Lys Ala Thr Glu 515 520 525 Glu Met 530 Ser Ser Phe Met Pro Asp Thr lie lie Ala Leu Gly Gly Thr 535 540 Pro Glu 545 Met Ser Ser Ala Lys Leu Met Trp Val Leu Tyr Glu His Pro 550 555 560 Glu Val Lys Phe Glu Asp Leu Ala lie Lys Phe Met Asp lie Arg Lys 565 570 575 Arg lie Tyr Thr Phe Pro Lys Leu Gly Lys Lys Ala Met Leu Val Ala 580 585 590 lie Thr Thr Ser Ala Gly Ser Gly Ser Glu Val Thr Pro Phe Ala Leu 595 600 605 Val Thr 610 Asp Asn Asn Thr Gly Asn Lys Tyr Met Leu Ala Asp Tyr Glu 615 620 Met Thr 625 Pro Asn Met Ala lie Val Asp Ala Glu Leu Met Met Lys Met 630 635 640 Pro Lys Gly Leu Thr Ala Tyr Ser Gly lie Asp Ala Leu Val Asn Ser 645 650 655 lie Glu Ala Tyr Thr Ser Val Tyr Ala Ser Glu Tyr Thr Asn Gly Leu 660 665 670 Ala Leu Glu Ala lie Arg Leu lie Phe Lys Tyr Leu Pro Glu Ala Tyr 675 680 685 Lys Asn 690 Gly Arg Thr Asn Glu Lys Ala Arg Glu Lys Met Ala His Ala 695 700 Ser Thr 705 Met Ala Gly Met Ala Ser Ala Asn Ala Phe Leu Gly Leu Cys 710 715 720 His Ser Met Ala lie Lys Leu Ser Ser Glu His Asn lie Pro Ser Gly 725 730 735 He Ala Asn Ala Leu Leu lie Glu Glu Val lie Lys Phe Asn Ala Val 740 745 750 Asp Asn Pro Val Lys Gin Ala Pro Cys Pro Gin Tyr Lys Tyr Pro Asn 755 760 765 Thr lie 770 Phe Arg Tyr Ala Arg lie Ala Asp Tyr lie Lys Leu Gly Gly 775 780 Asn Thr 785 Asp Glu Glu Lys Val Asp Leu Leu lie Asn Lys lie His Glu 790 795 800 126 201033370

Leu Lys Lys Ala Leu Asn lie Pro Thr Ser lie Lys Asp Ala Gly Val 805 810 815

Leu Glu Glu Asn Phe Tyr Ser Ser Leu Asp Arg lie Ser Glu Leu Ala 820 825 830

Leu Asp Asp Gin Cys Thr Gly Ala Asn Pro Arg Phe Pro Leu Thr Ser 835 840 845

Glu lie Lys Glu Met Tyr lie Asn Cys Phe Lys Lys Gin Pro 850 855 860

<210> 82 <211> 453 <212> PRT <213> Vibrio fluvialis <400> 82

Met Asn Lys Pro Gin Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 15 10 15

Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gin Arg Gly Thr Val Val 20 25 30

Val Thr His Gly Glu Gly Pro Tyr lie Val Asp Val Asn Gly Arg Arg 35 40 45

Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 50 55 60

His Lys Gly Leu lie Asp Ala Ala Lys Ala Gin Tyr Glu Arg Phe Pro 65 70 75 80

Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gin Thr Val Met Leu 85 90 95

Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 100 105 110

Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 115 120 125

Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gin Lys Arg Lys lie Leu 130 135 140

Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 145 150 155 160

Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 165 170 175

Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 180 185 190

Thr Glu Glu Gin Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 195 200 205 127 201033370

He Gin Arg Glu Gly Ala Asp Thr lie Ala Gly Phe Phe Ala Glu Pro 210 215 220

Val Met Gly Ala Gly Gly Val lie Pro Pro Ala Lys Gly Tyr Phe Gin 225 230 235 240

Ala lie Leu Pro lie Leu Arg Lys Tyr Asp lie Pro Val lie Ser Asp 245 250 255

Glu Val lie Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 260 265 270

Thr Tyr Asp Phe Thr Pro Asp Ala lie lie Ser Ser Lys Asn Leu Thr 275 280 285

Ala Gly Phe Phe Pro Met Gly Ala Val lie Leu Gly Pro Glu Leu Ser 290 295 300 ❹

Lys Arg Leu Glu Thr Ala lie Glu Ala lie Glu Glu Phe Pro His Gly 305 310 315 320

Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala lie Ala Leu Lys Ala 325 330 335 lie Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 340 345 350

Ala Pro Arg Phe Glu Glu Arg Leu Lys His lie Ala Glu Arg Pro Asn 355 360 365 lie Gly Glu Tyr Arg Gly lie Gly Phe Met Trp Ala Leu Glu Ala Val 370 375 380

Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser 385 390 395 400

Glu Arg lie Ala Asn Thr Cys Thr Asp Leu Gly Leu lie Cys Arg Pro 405 410 415

Leu Gly Gin Ser Val Val Leu Cys Pro Pro Phe lie Leu Thr Glu Ala 420 425 430

Gin Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 435 440 445

Phe Ala Glu Val Ala 450 <210> 83

<211> 449 <212> PRT <213> Bacillus weihenstephanensis <400> 83

Met Gin Ala Thr Glu Gin Thr Gin Ser Leu Lys Lys Thr Asp Glu Lys 15 10 15 128

Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu 20 25 30 lie lie Thr Lys Ala Glu Gly Ala Trp Val Thr Asp lie Asp Gly Asn 35 40 45

Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr 50 55 60

Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gin Leu Glu Glu Met 65 70 75 80

Pro Tyr Phe Pro Leu Thr Gin Ser His Val Pro Ala lie Lys Leu Ala 85 90 95

Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val lie Phe Phe Ser 100 105 110

Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys lie Ala Arg Gin 115 120 125

Tyr His Gin Gin Lys Gly Asp His Gly Arg Tyr Lys Phe lie Ser Arg 130 135 140

Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly 145 150 155 160

Gin Ala Gin Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gin Gly Phe Leu 165 170 175

His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr 180 185 190

Leu Ala Ser Ala Glu Glu lie Asp Arg Val Met Thr Trp Glu Leu Ser 195 200 205

Gin Thr Val Ala Gly Val lie Met Glu Pro lie lie Thr Gly Gly Gly 2X0 215 220 lie Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu lie Cys 225 230 235 240

Glu Lys His Gly Ala Leu Leu lie Cys Asp Glu Val lie Cys Gly Phe 245 250 255

Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro 260 265 270

Asp lie lie Thr Met Ala Lys Gly lie Thr Ser Ala Tyr Leu Pro Leu 275 280 285

Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser 290 295 300

Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro 305 310 315 320 129

Ala Ala Cys Ala Leu Ala Leu 325 Lys Asn Leu Glu lie Met Glu Asn Glu 330 335 Lys Leu lie Glu Arg Ser Lys 340 Glu Leu Gly Glu Arg Leu Leu Tyr Glu 345 350 Leu Glu Asp Val Lys Glu His 355 Pro Asn Val Gly Asp Val Arg Gly Lys 360 365 Gly Leu Leu Leu Gly lie Glu 370 375 Leu Val Glu Asp Lys Gin Thr Lys Glu 380 Pro Ala Ser lie Glu Lys Met 385 390 Asn Lys Val lie Asn Ala Cys Lys Glu 395 400 Lys Gly Leu lie Lie Gly Lys 405 Asn Gly Asp Thr Val Ala Gly Tyr Asn 410 415 Asn lie Leu Gin Leu Ala Pro 420 Pro Leu Ser lie Thr Glu Glu Asp Phe 425 430 Thr Phe lie Val Lys Thr Met 435 Gin Lys Glu Cys Leu Ser Arg lie Asn Gly 440 445 <210> 84 <211> 456

<212> PRT <213> Pseudomonas aeruginosa <400> 84

Met Asn Ser Gin lie Thr Asn 1 5 Ala Lys Thr Arg Glu Trp Gin Ala Leu 10 15 Ser Arg Asp His His Leu Pro 20 Pro Phe Thr Asp Tyr Lys Gin Leu Asn 25 30 Glu Lys Gly Ala Arg lie lie 35 Thr Lys Ala Glu Gly Val Tyr lie Trp 40 45 Asp Ser Glu Gly Asn Lys lie 50 55 Leu Asp Ala Met Ala Gly Leu Trp Cys 60 Val Asn Val Gly Tyr Gly Arg 65 70 Glu Glu Leu Val Gin Ala Ala Thr Arg 75 80 Gin Met Arg Glu Leu Pro Phe 85 Tyr Asn Leu Phe Phe Gin Thr Ala His 90 95

Pro Pro Val Val Glu Leu Ala Lys Ala lie Ala Asp Val Ala Pro Glu 100 105 110 Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp 115 120 125 130

Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gin Pro 130 135 140

Gin Lys Lys Val Val lie Gly Arg Trp Asn Gly Tyr His Gly Ser Thr 145 150 155 160

Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gin Gly 165 170 175

Asp Phe Pro lie Pro Gly lie Val His lie Ala Gin Pro Tyr Trp Tyr 180 185 190

Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala 195 200 205

Glu Gin Leu Glu Lys Lys lie Leu Glu Val Gly Glu Glu Asn Val Ala 210 215 220

Ala Phe lie Ala Glu Pro lie Gin Gly Ala Gly Gly Val lie Val Pro 225 230 235 240

Pro Asp Thr Tyr Trp Pro Lys lie Arg Glu lie Leu Ala Lys Tyr Asp 245 250 255 lie Leu Phe lie Ala Asp Glu Val lie Cys Gly Phe Gly Arg Thr Gly 260 265 270

Glu Trp Phe Gly Ser Gin Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro 275 280 285 lie Ala Lys Gly Leu Thr Ser Gly Tyr lie Pro Met Gly Gly Val Val 290 295 300

Val Arg Asp Glu lie Val Glu Val Leu Asn Gin Gly Gly Glu Phe Tyr 305 310 315 320

His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu 325 330 335

Glu Asn lie Arg lie Leu Arg Glu Glu Lys lie lie Glu Lys Val Lys 340 345 350

Ala Glu Thr Ala Pro Tyr Leu Gin Lys Arg Trp Gin Glu Leu Ala Asp 355 360 365

His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu 370 375 380

Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg Phe Thr Asp Lys Gly 385 390 395 400

Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu lie Met 405 410 415

Arg Ala Val Gly Asp Thr Met lie lie Ser Pro Pro Leu Val lie Asp 420 425 430 131

Pro Ser Gin lie Asp Glu Leu lie Thr Leu Ala Arg Lys Cys Leu Asp 435 440 445

Gin Thr Ala Ala Ala Val Leu Ala 450 455 <210> 85

<211> 538 <212> PRT <213> Penicillium Chrysogenum <400> 85

Met Thr Ala Leu Arg lie Ser Thr Thr Leu Ser Arg Lys Ala Leu Thr 15 10 15

Ala Cys Arg Pro Arg Leu Phe Leu Gly Val Arg Thr Ser Val Arg Pro 20 25 30

Trp Gin Pro Phe Ser Thr Ser Thr Ser Thr Arg Tyr Ser lie Thr Thr 35 40 45

Pro Leu Lys Ser Leu Ala Pro Lys Val Glu Arg Ser Gly Ser Lys Leu 50 55 60

Tyr Lys Asp Ala Asp Ala Ala Val Ala Asp lie Lys Ser Gly Ser Thr 65 70 75 80 lie Leu Ser Ser Gly Phe Gly Leu Cys Gly Val Ala Glu Thr Leu lie 85 90 95

Asn Ala Met His Arg Arg Gly Ala Asp Gin Leu His Ser Leu Thr Ala 100 105 110

Val Ser Asn Asn Ala Gly Ala Ala Gly Lys Gly Gly Leu Ser Thr Leu 115 120 125

Ser Gin Asn Gly Gin lie Asn Arg Leu lie Leu Ser Tyr Leu Gly Asn 130 135 140

Asn Lys Ala Leu Glu Lys Lys Tyr Leu Thr Gly His lie Ala lie Glu 145 150 155 160

Leu Cys Pro Gin Gly Thr Leu Ala Glu Arg Leu Arg Ala Gly Gly Ala 165 170 175

Gly lie Pro Ala Phe Phe Thr Pro Thr Gly Glu Gly Lys lie Pro Val 180 185 190

Arg Met Asp Glu Ser Gly Lys Val Leu Glu Ser Gly Lys Pro Arg Glu 195 200 205

Thr Arg Glu Phe Asn Gly Lys Thr Tyr Leu Met Glu Glu Ala Leu Thr 210 215 220

Gly Asp Val Ala lie Leu Arg Ala Trp Lys Ala Asp Glu Ala Gly Asn 225 230 235 240 132

Cys Val Phe Arg Tyr Thr Thr Lys Ala Phe Gly Pro lie Met Ala Lys 245 250 255

Ala Ala Thr Leu Thr lie Val Glu Ala Glu Asn lie Val Pro lie Gly 260 265 270

Ser lie Asp Pro Asn Asp Val Asp Leu Pro Gly lie Phe Val Asp Arg 275 280 285 lie Val Pro Ala Thr Asp Asp Lys His lie Glu lie Arg Lys Leu Arg 290 295 300

Ser Gly Glu Thr Thr Val Ala Gly Ser Gly Lys Asp Glu Ala Arg lie 305 310 315 320

Gin Arg Glu Leu lie Gly Arg Arg Ala Ala Lys Glu Leu Lys Pro Gly 325 330 335

Phe Tyr Val Asn Leu Gly Val Gly lie Pro Thr Leu Ala Pro Ser Phe 340 345 350

Leu Pro Lys Asp Val Lys Val Trp lie Gin Ser Glu Asn Gly lie Leu 355 360 365

Gly Met Gly Asp Tyr Pro Thr Glu Gin Glu Leu Asp Pro Asp lie lie 370 375 380

Asn Ala Gly Lys Glu Thr Val Thr Leu Val Pro Gly Ala Ala Thr Phe 385 390 395 400

Asp Ser Ser Glu Ser Phe Gly Met lie Arg Gly Gly His Val Asp Val 405 410 415

Ser lie Leu Gly Ala Leu Gin Val Ser Ala Asn Gly Asp Leu Ala Asn 420 425 430

Tyr Met lie Pro Gly Lys Val Phe Lys Gly Met Gly Gly Ala Met Asp 435 440 445

Leu lie Ser Asn Pro Glu Lys Thr Lys lie Val Val Ala Thr Ser His 450 455 460

Val Ala Lys Asp Gly Ser Pro Lys lie Val Gin Lys Cys Ser Leu Pro 465 470 475 480

Leu Thr Gly Ala Asn Val Val Ser Thr lie lie Thr Asp Leu Cys Val 485 490 495

Phe Gin Val Asp Arg Ala Thr Gly Glu Leu Thr Leu Thr Glu Leu Ala 500 505 510

Pro Gly Val Glu Val Glu Glu Val Gin Ser Lys Thr Asp Ala Lys Phe 515 520 525

Thr lie Ala Asp Thr Leu Glu lie Met Glu 133 48 48 535 530 <210> 86 <211> 1362

<212> DNA <213> vibrio fluvialis <220><221> CDS <222> (1) . . (1362) <400> 86 atg aac aaa ccg caa age tgg gaa gee egg gee gag acc Tat teg etc

Met Asn Lys Pro Gin Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 15 10 15 tat ggt ttc acc gac atg cct teg ctg cat cag ege ggc aeg gtc gtc

Tyr Giy Phe Thr Asp Met Pro Ser Leu His Gin Arg Gly Thr Val Val 20 25 30 gtg acc cat ggc gag gga ccc tat ate gtc gat gtg aat ggc egg cgt Val Thr His Gly Glu Gly Pro Tyr lie Val Asp Val Asn Gly Arg Arg 35 40 45 tat ctg gac gee aac teg ggc ctg tgg aac atg gtc geg ggc ttt gac Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 50 55 60 cac aag ggg ctg ate gac gee gee aag gee caa Tac gag cgt ttt ccc His Lys Gly Leu lie Asp Ala Ala Lys Ala Gin Tyr Glu Arg Phe Pro 65 70 75 80 ggt tat cac gee ttt ttc ggc ege atg tee gat cag aeg gta atg ctg Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gin Thx Val Met Leu 85 90 95 teg gaa aag ctg gtc gag gtg teg ccc ttt gat teg ggc egg gtg ttc Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 100 105 110 tat aca aac teg ggg Tee gag geg aat gac acc atg gtc aag atg eta Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 115 120 125 tgg ttc ctg cat gca gee gag ggc aaa ccg caa aag ege aag ate ctg Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gin Lys Arg Lys lie Leu 130 135 140 acc ege tgg aac gee tat cac ggc gtg acc gee gtt teg gee age atg Thr Arg Trp Asn Ala Tyr His Gly Val Hir Ala Val Ser Ala Ser Met 145 150 155 160 acc ggc aag Ccc tat aat teg gtc ttt ggc ctg ccg ctg ccg ggc ttt Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 165 170 175 gtg cat ctg acc tgc ccg cat tac tgg ege tat ggc gaa gag ggc gaa Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 180 185 190 acc gaa gag cag ttc gtc gee ege etc gee ege gag ctg gag gaa aeg Thr Glu Glu Gin Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 195 200 203 ate cag ege gag ggc gee gac acc ate gee ggt ttc ttt gee gaa ccg lie Gin Arg Glu Gly Ala Asp Thr lie Ala Gly Phe Phe Ala Glu Pro 210 215 220 gtg atg ggc geg ggc ggc gtg att ccc ccg gee aag ggc Tat ttc cag Val Met Gly Ala Gly Gly Val lie Pro Pro Ala Lys Gly Tyr Phe Gin 225 230 235 240 geg ate ctg cca ate ctg ege aaa tat gac ate ccg gtc ate teg gac 96 144 192 240 288 336 384 432 480 528 576 6 24 672 720 768 134 816

Ala lie Leu Pro lie Leu Arg Lys Tyr Asp lie Pro Val lie Ser Asp 245 250 255 gag gtg ate tgc ggt ttc gga ege acc ggt aac acc tgg ggc tgc gtg Glu Val lie Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 260 265 270 acc tat gac ttt aca ccc gat gca ate ate teg tee aag aat ett aca

Thr Tyr Asp Phe Thr Pro Asp Ala lie lie Ser Ser Lys Asn Leu Thr 275 280 285 geg ggc ttt ttc ccc atg ggg geg gtg ate ett ggc ccg gaa ett tee

Ala Gly Phe Phe Pro Met Gly Ala Val lie Leu Gly Pro Glu Leu Ser 290 295 300 aaa egg ctg gaa acc gca ate gag geg ate gag gaa ttc ccc cat ggc

Lys Arg Leu Glu Thr Ala lie Glu Ala lie Glu Glu Phe Pro His Gly 305 310 315 320 ttt acc gee teg ggc cat ccg gtc ggc tgt get att geg ctg aaa gca

Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala lie Ala Leu Lys Ala 325 330 335 ate gac gtg gtg atg aat gaa ggg ctg get gag aac gtc ege ege ett lie Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 340 345 350 gee ccc cgt ttc gag gaa agg ctg aaa cat ate gee gag ege ccg aac

Ala Pro Arg Phe Glu Glu Arg Leu Lys His lie Ala Glu Arg Pro Asn 355 360 365 ate ggt gaa tat ege ggc ate ggc ttc atg tgg geg ctg gag get gtc lie Gly Glu Tyr Arg Gly lie Gly Phe Met Trp Ala Leu Glu Ala Val 370 375 380 aag gac aag gca age aag aeg ccg ttc gac ggc aac ctg teg gtc age

Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser 385 390 395 400 gag cgt ate gee aat acc tgc acc gat ctg ggg ctg att tgc egg ccg

Glu Arg lie Ala Asn Thr Cys Thr Asp Leu Gly Leu lie Cys Arg Pro 405 410 415 ett ggt cag tee gtc gtc ett tgt ccg ccc ttt ate ctg acc gag geg

Leu Gly Gin Ser Val Val Leu Cys Pro Pro Phe lie Leu Thr Glu Ala 420 425 430 cag atg gat gag atg ttc gat aaa etc gaa aaa gee ett gat aag gtc

Gin Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 435 440 445 ttt gee gag gtt gee tga 1362

Phe Ala Glu Val Ala 450 <210> 87 <211> 453

<212> PRT <213> vibrio fluvialis <400> 87

Met Asn Lys Pro Gin Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser 15 10 15

Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gin Arg Gly Thr Val 20 25 30

Val Thr His Gly Glu Gly Pro Tyr lie Val Asp Val Asn Gly Arg 35 40 45 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344

Leu Val Arg 135

Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 50 55 60 His Lys 65 Gly Leu lie Asp Ala Ala Lys Ala Gin Tyr Glu Arg Phe Pro 70 75 80 Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gin Thr Val Met Leu 85 90 95 Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 100 105 110 Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 115 120 125 Trp Phe 130 Leu His Ala Ala Glu Lys Pro Gin Lys Arg Lys lie Leu 135 140 Thr Arg 145 Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 150 155 160 Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 165 170 175 Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 180 185 190 Thr Glu Glu Gin Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 195 200 205 He Gin 210 Arg Glu Gly Ala Asp Thr lie Ala Gly Phe Phe Ala Glu Pro 215 220 Val Met 225 Gly Ala Gly Gly Val lie Pro Pro Ala Lys Gly Tyr Phe Gin 230 235 240 Ala lie Leu Pro lie Leu Arg Lys Tyr Asp lie Pro Val lie Ser Asp 245 250 255 Glu Val lie Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 260 265 270 Thr Tyr Asp Phe Thr Pro Asp Ala lie lie Ser Ser Lys Asn Leu Thr 275 280 285

Ala Gly Phe Phe Pro Met Gly Ala Val lie Leu Gly Pro Glu Leu Ser 290 295 300

Lys Arg Leu Glu Thr Ala lie Glu Ala lie Glu Glu Phe Pro His Gly 305 310 315 320

Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala lie Ala Leu Lys Ala 325 330 335 lie Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 340 345 350 136 201033370

Ala Pro Arg Phe Glu Glu Arg Leu 355 360

Lys His lie Ala Glu Arg Pro Asn 365 lie Gly Glu Tyr Arg Gly lie Gly 370 375

Phe Met Trp Ala Leu Glu Ala Val 380

Lys Asp Lys Ala Ser Lys Thr Pro 385 390

Phe Asp Gly Asn Leu Ser Val Ser 395 400

Glu Arg lie Ala Asn Thr Cys Thr 405

Asp Leu Gly Leu lie Cys Arg Pro 410 415

Leu Gly Gin Ser Val Val Leu Cys 420

Pro Pro Phe lie Leu Thr Glu Ala 425 430

Gin Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 435 440 445

Phe Ala Glu Val Ala 450 <210> 88 <211> 1362 <212> DNA <213> Artificial <220><223> Vibrio f luvialis JS17 omega-aminotransferase codon optimised gene <400> 88 atgaataaac cacagtcttg ggaagctcgt gctgaaacct atagcctgta cggctttacc 60 gatatgccgt ctctgcacca gcgtggtact gtagtggtaa cgcacggtga gggcccgtac 120 atcgtggacg ttaatggccg ccgttacctg gatgcaaaca gcggcctgtg gaacatggtt 180 gcgggcttcg accacaaagg cctgatcgat gccgcaaaag cgcagtacga acgcttcccg 240 ggttatcacg cgttctttgg ccgtatgagc gaccagactg tgatgctgag cgaaaaactg 300 gttgaagtgt ccccgttcga tagcggtcgt gtcttttaca ctaactctgg cagcgaggct 360 aacgatacca tggttaagat gctgtggttc ctgcacgcag cggaaggcaa acctcagaaa 420 cgtaaaattc tgacccgttg gaacgcttat cacggtgtga ctgctgtttc cgcatctatg 480 accggtaaac cgtataacag cgtgttcggt ctgccgctgc ctggcttcgt gcatctgacc 540 tgcccgcact actggcgtta tggtgaggaa ggcgaaactg aggaacagtt cgtggcgcgt 600 ctggctcgtg aactggaaga aaccattcaa cgcgaaggtg cagatactat cgcgggcttc 660 tttgcggagc ctgttatggg tgccggcg gt gtgattccgc cggcgaaggg ctatttccag 720 gcaatcctgc cgatcctgcg caagtacgac attccggtta tttctgacga agtgatctgc 780 ggcttcggcc gcaccggtaa cacctggggc tgcgtgacgt atgacttcac tccggacgca 840 atcattagct ctaaaaacct gactgcgggt ttcttcccta tgggcgccgt aatcctgggc 900 ccagaactgt ctaagcgcct ggaaaccgcc atcgaggcaa tcgaagagtt cccgcacggt 960 ttcactgcta gcggccatcc ggtaggctgc gcaatcgcgc tgaaggcgat cgatgttgtc 1020 atgaacgagg gcctggcgga aaacgtgcgc cgcctggcgc cgcgttttga agaacgtctg 1080 aaacacattg ctgagcgccc gaacattggc Gaatatcgcg gcatcggttt catgtgggcc 1140 137 1200 1200

❷ 201033370 ctggaagcag ttaaagataa agctagcaag accccgttcg acggcaacct gtccgtgagc gaacgtatcg ctaatacctg tacggacctg ggtctgatct gccgtccgct gggtcagtcc gtagttctgt gcccaccatt tatcctgacc gaagcgcaga tggatgaaat gttcgataaa ctggagaaag ctctggataa agtgttcgct gaagtcgcgt aa < 210 > 89 < 211 > 1350 < 212 > DNA < 213 > Baci1lus weihenstephanensis < 220 ><221> CDS <222> (1) . . (1350) <400> 89 gtg caa gcg acg gag caa aca caa agt ttg aaa aaa aca gat gaa aag

Val Gin Ala Thr Glu Gin Thr Gin Ser Leu Lys Lys Thr Asp Glu Lys 15 10 15 tac ctt tgg cat gcg atg aga gga gca gcc cct agt cca acg aat tta

Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu 20 25 30 att ate aca aaa gca gaa ggg gca tgg gtg acg gat att gat gga aac lie lie Thr Lys Ala Glu Gly Ala Trp Val Tlir Asp lie Asp Gly Asn 35 40 45 cgt tat tta gac ggt atg tcc ggt ctt tgg tgc gtg aat gtt ggg tat Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr 50 55 60 ggt ega aaa gaa ctt gca aga gcg gcg ttt gaa Cag ctt gaa gaa atg Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gin Leu Glu Glu Met 65 70 75 80 ccg tat ttc cct ctg act caa agt cat gtt cct get att aaa tta gca Pro Tyr Phe Pro Leu Thr Gin Ser His Val Pro Ala lie Lys Leu Ala 85 90 95 gaa aaa ttg aat gaa tgg ctt gat gat gaa tac gtc att ttc ttt tet Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val lie Phe *Phe Ser 100 105 110 aac agt gga teg Gaa gcg aat gaa aca gca ttt aaa att get cgt caa Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys lie Ala Arg Gin 115 120 125 tat cat caa caa aaa ggt gat cat gga ege tat aag ttt att tcc ege Tyx His Gin Gin Lys Gly Asp Hi s Gly Arg Tyr Lys Phe lie Ser Arg 130 135 140 tac ege get tat cac ggt aac tea atg gga get ctt gca gca aca ggt Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly 145 150 155 160 caa gca Cag ega aag tat aaa tat gaa cca etc ggg caa gga ttc ctg Gin Ala Gin Arg Lys Tyx Lys Tyr Glu Pro Leu Gly Gin Gly Phe Leu 165 170 175 cat gta gca ccg cct gat acg tat ega aat cca gag gat gtt cat aca His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr 180 185 190 ctg gca agt get gag gaa ate gat cgt gtc atg aca tgg gag tta age Leu Ala Ser Ala Glu Glu lie Asp Arg Val Met Thr Trp Glu Leu Ser 195 200 205 caa aca gta gcc ggt gtg att atg gag cca ate att act ggg ggc gga Gin Thr Val Ala Gly Val lie Met Glu Pro lie lie Thr Gly Gly Gly 1260 1320 1362 48 96 144 192 240 288 336 384 432 480 528 576 624 138 672 201033370 210 215 220 att tta atg cct cct gat gga tat atg gga aaa gta aaa gaa att tgc 720 lie Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu lie Cys 225 230 235 240 gag aag cac ggt gcg Ttg etc att tgt gat gaa gtt ata tgt gga ttt 768

Glu Lys His Gly Ala Leu Leu lie Cys Asp Glu Val lie Cys Gly Phe 245 250 255 ggc egg aca ggg aag cca ttt gga ttt atg aat tat ggc gtc aaa cca 816

Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro 260 265 270 gat ate att aca atg gca aaa ggt att aca agt gcg tat ett cct ttg 864

Asp lie lie Thr Met Ala Lys Gly lie Thr Ser Ala Tyr Leu Pro Leu 275 280 285 tea gca aca gca gtt aga ega gag gtt tat gag gca ttc gta ggt agt 912

Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser 290 295 300 gat gat tat gat ege ttc ege cat gta aat aeg ttc gga ggg aat cct 960

Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro

305 310 315 320 get get tgc get tta get ttg aag aat tta gaa att atg gag aat gag 1008 Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Glu lie Met Glu Asn Glu 325 330 335 aaa etc att gaa cgt tee aaa gaa ttg ggt Gaa ega ctg tta tat gag 1056 Lys Leu lie Glu Arg Ser Lys Glu Leu Gly Glu Arg Leu Leu Tyr Glu 340 345 350 eta gag gat gata aaa gag cat cca aac gta ggg gat gtt ege gga aag 1104 Leu Glu Asp Val Lys Glu His Pro Asn Val Gly Asp Val A: rg Gly Lys 355 360 365 ggc ett ett tta ggc att gaa eta gtg gaa gat aag caa aca aaa gaa 1152 Gly Leu Leu Leu Gly lie Glu Leu Val Glu Asp Lys Gin Thr Lys Glu 370 375 380 Ccg get tee att gaa aag atg aac aaa gtc ate aat get tgt aaa gaa 1200 Pro Ala Ser lie Glu Lys Met Asn Lys Val lie Asn Ala Cys Lys Glu 385 390 395 400

Aaa ggt eta att att ggt aaa aat ggt gac act gtc gca ggt tac aat 1248 Lys Gly Leu lie lie Gly Lys Asn Gly Asp Thr Val Ala Gly Tyr Asn 405 410 415 aat att ttg cag ett gca cct cca tta age ate aca gag gaa Gac ttt 1296 Asn lie Leu Gin Leu Ala Pro Pro Leu Ser lie Thr Glu Glu Asp Phe 420 425 430 act ttt ate gtt aaa aca atg aaa gaa tgt tta tee ege att aac ggg 1344 Thr Phe lie Val Lys Thr Met Lys Glu Cys Leu Ser Arg lie Asn Gly 435 440 445 cag taa 1350

Gin <210> 90

<211> 449 <212> PRT <213> Bacillus weihenstephanensis <400> 90

Val Gin Ala Thr Glu Gin Thr Gin Ser Leu Lys Lys Thr Asp Glu Lys 15 10 15

Tyr Leu Trp His Ala Met Arg Gly Ala Ala Pro Ser Pro Thr Asn Leu 139 20 25 30 lie lie Thr Lys Ala Glu Gly Ala Trp Val Thr Asp lie Asp Gly Asn 35 40 45

Arg Tyr Leu Asp Gly Met Ser Gly Leu Trp Cys Val Asn Val Gly Tyr 50 55 60

Gly Arg Lys Glu Leu Ala Arg Ala Ala Phe Glu Gin Leu Glu Glu Met 65 70 75 80

Pro Tyr Phe Pro Leu Thr Gin Ser His Val Pro Ala lie Lys Leu Ala 85 90 95

Glu Lys Leu Asn Glu Trp Leu Asp Asp Glu Tyr Val lie Phe Phe Ser 100 105 110

Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys lie Ala Arg Gin 115 120 125

Tyr His Gin Gin Lys Gly Asp His Gly Arg Tyr Lys Phe lie Ser Arg 130 135 140

Tyr Arg Ala Tyr His Gly Asn Ser Met Gly Ala Leu Ala Ala Thr Gly 145 150 155 160

Gin Ala Gin Arg Lys Tyr Lys Tyr Glu Pro Leu Gly Gin Gly Phe Leu 165 170 175

His Val Ala Pro Pro Asp Thr Tyr Arg Asn Pro Glu Asp Val His Thr 180 185 190

Leu Ala Ser Ala Glu Glu lie Asp Arg Val Met Thr Trp Glu Leu Ser 195 200 205

Gin Thr Val Ala Gly Val lie Met Glu Pro lie lie Thr Gly Gly Gly 210 215 220 lie Leu Met Pro Pro Asp Gly Tyr Met Gly Lys Val Lys Glu lie Cys 225 230 235 240

Glu Lys His Gly Ala Leu Leu lie Cys Asp Glu Val lie Cys Gly Phe 245 250 255

Gly Arg Thr Gly Lys Pro Phe Gly Phe Met Asn Tyr Gly Val Lys Pro 260 265 270

Asp lie lie Thr Met Ala Lys Gly He Thr Ser Ala Tyr Leu Pro Leu 275 280 285

Ser Ala Thr Ala Val Arg Arg Glu Val Tyr Glu Ala Phe Val Gly Ser 290 295 300

Asp Asp Tyr Asp Arg Phe Arg His Val Asn Thr Phe Gly Gly Asn Pro 305 310 315 320 140 201033370

Ala Ala Cys Ala Leu 325 Ala Leu Lys Asn Leu 330 Glu lie Met Glu Asn 335 Glu Lys Leu lie Glu 340 Arg Ser Lys Glu Leu 345 Gly Glu Arg Leu Leu 350 Tyr Glu Leu Glu Asp 355 Val Lys Glu His Pro 360 Asn Val Gly Asp Val 365 Arg Gly Lys Gly Leu 3 70 Leu Leu Gly lie Glu 375 Leu Val Glu Asp Lys 380 Gin Thr Lys Glu Pro 385 Ala Ser lie Glu Lys 390 Met Asn Lys Val lie 395 Asn Ala Cys Lys Glu 400 Lys Gly Leu Lie lie 405 Gly Lys Asn Gly Asp 410 Thr Val Ala Gly Tyr 415 Asn Asn lie Leu Gin 420 Leu Ala Pro Pro Leu 425 Ser lie Thr Glu Glu 430 Asp Phe Thr Gin Phe lie 435 Val Lys Thr Met Lys 440 Glu Cys Leu Ser Arg 445 He Asn Gly ❹ <210> 91 <211> 1350 <212> DNA <213> Artificial <220><223> B. weihenstephanensis KBAB4 aminotransferase codon-optimised gene <400> 91 acgaaaaata Tctgtggcac 60 ttaccaaagc tgaaggcgcg 120 tgagcggcct gtggtgtgtt 180 ttgaacaact ggaagaaatg 240 aactggcgga aaaactgaac 300 ctggctccga agcaaacgaa 360 gtgaccacgg ccgctataaa 420 gtgcgctg Gc tgctaccggt 480 gttttctgca cgttgcacca 540 cttctgccga agaaatcgat 600 ttatcatgga acctattatt 660 gtaaagtcaa ggaaatctgc 720 gtggcttcgg tcgcaccggc 780 ttattaccat ggctaaaggc 840 gccgcgaagt ttatgaagcg 900

atgcaggcta ccgaacaaac ccaatctctg aaaaagactg gcgatgcgcg gtgcagctcc gtctccgacc aacctgatta tgggtgaccg acattgacgg taaccgttat ctggatggca aatgtcggtt atggccgtaa ggagctggcg cgcgcggcat ccgtacttcc cgctgactca aagccatgtg ccggctatca gaatggctgg acgacgaata cgtgattttc ttctctaatt accgcattca aaatcgcccg tcaatatcac cagcagaaag ttcatcagcc gttatcgtgc ataccatggt aattctatgg caggctcagc gcaaatacaa gtacgaaccg ctgggtcagg ccggatacct accgtaaccc ggaagacgtc cacaccctgg cgtgttatga cctgggagct gtcccagact gttgcgggtg accggtggtg gcattctgat gccgccggac ggttatatgg gaaaaacacg gcgcgctgct gatctgcgat gaagttatct aaaccatttg gcttcatgaa ttatggcgta aaacctgaca attacttccg cttatctgcc gctgagcgcg accgcagttc 141 tttgttggtt ctgatgatta cgaccgtttc cgtcatgtaa acacgtttgg cggtaaccca 960 gcggcatgtg cgctggcgct gaaaaacctg gaaatcatgg aaaacgaaaa gctgatcgaa 1020 cgtagcaaag aactgggtga acgtctgctg tacgaactgg aagatgtcaa agaacacccg 1080 aacgtgggcg atgttcgcgg taaaggcctg ctgctgggta ttgaactggt tgaagacaaa 1140 cagaccaagg aaccggcttc cattgaaaag atgaacaaag tgattaacg c gtgcaaagag 1200 aaaggcctga teattggtaa gaacggtgat accgtggcag gttataacaa cattctgcag 1260 ctggcgccgc ctctgagcat cactgaagaa gatttcacct teategteaa aactatgaag 1320 gagtgcctga gccgcatcaa tggtcagtaa 1350 <210> 92 <211> 255

<212> PRT <213> Saccharomyces cerevisiae; Hydroxyacyl-thioester dehydratase type 2, mitochondrial (P38790) <400> 92

Met Ala 1 Ala Val Gly Val Ala Val 50

Glu Leu Arg Val Leu Val Ala Val Lys Arg Val lie Asp Tyr 5 10 15 Lys lie Arg Val Lys Pro Asp Arg Thr Gly Val Val Thr Asp 20 25 30

Lys His Ser Met Asn Pro Phe Cys Glu 35 40 Arg Leu Lys Glu Lys Lys Leu Val Lys

He Ala Val Glu Glu 45 55

Glu Val He Ala Val 60

Ser Cys Gly Pro Ala Gin Cys Gin Glu Thr lie Arg Thr Ala Leu Ala 65 70 75 - 80

Met Gly Ala Asp Arg Gly lie His Val Glu Val Pro Pro Ala Glu Ala 85 90 95

Glu Arg Leu Gly Pro Leu Gin Val Ala Arg Val Leu Ala Lys Leu Ala 100 105 110

Glu Lys Glu Lys Val Asp Leu Val Leu Leu Gly Lys Gin Ala lie Asp 115 120 125

Asp Asp Cys Asn Gin Thr Gly Gin Met Thr Ala Gly Phe Leu Asp Trp 130 135 140

Pro Gin Gly Thr Phe Ala Ser Gin Val Thr Leu Glu Gly Asp Lys Leu 145 150 155 160

Lys Val Glu Arg Glu lie Asp Gly Gly Leu Glu Thr Leu Arg Leu Lys 165 170 175

Leu Pro Ala Val Val Thr Ala Asp Leu Arg Leu Asn Glu Pro Arg Tyr 180 185 190

Ala Thr Leu Pro Asn lie Met Lys Ala Lys Lys Lys Lys lie Glu Val 195 200 205 142 201033370 lie Lys 210

Pro Gly Asp Leu Gly Val Asp Leu Thr Ser Lys Leu Ser Val 215 220 lie Ser 225

Val Glu Asp Pro Pro Gin Arg Thr Ala Gly Val Lys Val Glu 230 235 240

Thr Thr

Glu Asp Leu Val Ala Lys Leu Lys Glu lie Gly Arg lie 245 250 255 <210> 93 <211> 860 <212> DNA <213> artificial <220><223> Microscilla marina ATCC 23134 Peroxisomal trans -2-enoyl-CoA reductase; EC = 1.3.1.38 (A1ZFB1) < 400 > 93 ggtaccggat ccctaacagg aggaattaac catggtccag aaagacctgt tcaaagacaa 60 agttgttctg gtaactggcg gtcgttctgg tatcggttac gcaatttctc agatgatgct 120 ggaactgggt gcgaaagttg ttatcgcttc tcgcaaagag gatctgctga aacaggctgc 180 tgaagaactg tctcagtacg gtgaatgctc ctacctggct tgcgacatcc gtgaatctga 240 ccagcgtact gcgctgatgg agaagatcaa agctgacaac ggtcgtctgg atatcctggt 300 taacaacgct ggcggtcagt tcccggcacc ggcagaaacc atctctgaaa acggctggga 360 cgctgttatc aacaacaacc tgaacggtac tttccacatg tcctctctga tggcgcgtca 420 cttcttcatc ccgcagaaag aaggttgcat catcaacatc atcgctaaca tctaccgcgg 480 tttcccgtct atggttcaca ccggtgcagc acgtgctggc gttgaaaacc tgaccaaaac 540 tctggcggtt gagtggggcg actacaacat ccgcgttaac gcgattgctc cgggtactat 600 cgaatcttcc ggtctggata cttacccgaa g ccggtacag gacatcctgg gtgaagcgcg 660 tgctgcagta ccactgaaac gcttcggtac tgttactgaa atcgctaaca ccacttgctt 720 cctggcttct ccgctggctt cttacatctc cggtgtttct ctgtacgttg acggtgcaca 780 gcacctgaac ggtggtgacc cgatgaagct gactcgcctg atgcgtgcat tcaccaagtc 840 tcgtgagctc taataagctt 860

<210> 94 <211> 273 <212> PRT <213> Microscilla itarina <400> 94 Met Val Gin Lys Asp 1 5 AIGC 23134 i^raxiscmal trar^-2-erio/l-CkA reductase; BO=l.3.1.38 (Ά1ΖΕΒ1)

Gly Arg Ser Gly lie 20

Gly Ala Lys Val Val 35 Ala Ala Glu Glu Leu 50

Leu Phe Lys Asp Lys Val 10 Gly Tyr Ala lie Ser Gin 25 lie Ala Ser Arg Lys Glu 40 Ser Gin Tyr Gly Glu Cys 55

Val Met Asp Ser 60

Leu Val

Met Leu 30

Leu Leu 45

Tyr Leu

Thr 15 Glu Lys Ala

Gly Leu Gin Cys 143

Asp lie Arg Glu Ser 65 Asp Gin Arg Thr Ala Leu Met Glu Lys lie Lys 70 75 80 Ala Asp Asn Gly Arg 85 Leu Asp lie Leu Val Asn Asn Ala Gly Gly Gin 90 95 Phe Pro Ala Pro Ala 100 Glu Thr lie Ser Glu Asn Gly Trp Asp Ala Val 105 110 lie Asn Asn Asn Asn Leu 115 Asn Gly Thr Phe His Met Ser Ser Leu Met Ala 120 125 Arg His Phe Phe lie 130 Pro Gin Lys Glu Gly Cys lie lie Asn lie lie 135 140 Ala Asn lie Tyr Arg 145 Gly Phe Pro Ser Met Val His Thr Gly Ala Ala 150 155 160 Arg Ala Gly Val Glu 165 Asn Leu Thr Lys Thr Leu Ala Val Glu Trp Gly 170 175 Asp Tyr Asn lie Arg 180 Val Asn Ala lie Ala Pro Gly Thr lie Glu Ser 185 190 Ser Gly Leu Asp Thr 195 Tyr Pro Lys Pro Val Gin Asp lie Leu Gly Glu 200 205 Ala Arg Ala Ala Val 210 Pro Leu Lys Arg Phe Gly Thr Val Thr Glu lie 215 220 Ala Asn Thr Thr Cys 225 Phe Leu Ala Ser Pro Leu Ala Ser Tyr lie Ser 230 235 240 Gly Val Ser Leu Tyr 245 Val Asp Gly Ala Gin His Leu Asn Gly Gly Asp 250 255 Pro Met Lys Leu Thr 260 Thr Arg Leu Met Arg Ala Phe Thr Lys Ser Arg Glu 265 270 <210> 95 <211> 1241 <212> DNA <213> Artificial <220><223> Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052), Putative reductase Cbei_2086; EC=1.3.1 (A6LV73) < 400 > 95 ggtaccggat ccctaacagg aggaattaat catgatcttc aagccggaac tgatcaaagg 60 tatcgctaaa acttctcacc cgtacggttg ccgtaaagaa gttctgaacc agatcgaata 120 ctgcaaaaac gctaagcagt tccacggtcc gaagaaagtt ctgatcatcg gtgcttcttc 180 cggtttcggt ctggcaactc gcatttctct ggcgttcggt ggtgcgaaag cagacaccat 240 144 201033370 cggtgtttct ttcgaaaccg gtatcactga ccgccgtact ggtactgcag gctggtacaa 300 caacatcttc ttcaaagagt tcgcagaaaa agaaggtctg attgctaaaa acttcatcgg 360 tgacgcattc tctgacgaag ttaaagaaaa cgttatcaaa tacatcaaaa acgagttcgg 420 taagatcgat ctgctgatct actctctggc ttctccgcgc cgtaaagatc cgaaaaccgg 480 taacatctac gactccactc tgaaaaccac ttctggcgag ttccagggcc caaccatcga 540 catggaaact gacgaactgg ttactaccaa agttaactcc gcaactgaca aagaaatcga 600 agcgaccaag aaagtaatgg gtggtgaaga ttggtctgaa tggtgcaaac tgc tgctgga 660 aaacgactgc ctgtctgaca aagctatcac tatctcttac tcttacatcg gtgcttctcg 720 cacttacaaa atctaccgtg aaggtactat cggtgaagcg aagcgtcacc tggaaaacac 780 tgcgattcag atcgacaaag aatggcagaa gaagatcaac ggtaaagcat tcgtttctgt 840 taacaaagca atcgttacca aggcttctgc ttacatcccg tctttctctc tgtacgctgc 900 tgttctgtac aaagtaatga aagaaaaaaa tctgcacgaa aactgcatca tgcagatgca 960 gcgtatgttc gctgacaaaa tctacgctga aaatctgctg gagttcgacg actccggtcg 1020 tctgcgtatg gacgactggg aactgcgtga agatgtacag tctgaagtta acgaactgtg 1080 ggaaaaaatc actccggata acttcaagat cctgtctgac tacgacggtt acaagaaaga 1140 gttcatgcag ctgaacggct tcgaaatcga cggtgttaac tactctgaag atatcgacat 1200 cgaagcgctg aagcgcctgg aaccggagct ctaataagct t 1241 <210> 96 <211> 400

<212> PRT <213> Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052), Putative reductase Cbei_2086; EC=1.3.1 (A6LV73) <400> 96

Met lie Phe Lys Pro Glu Leu lie Lys Gly lie Ala Lys Thr Ser His 15 10 15

Pro Tyr Gly Cys Arg Lys Glu Val Leu Asn Gin lie Glu Tyr Cys Lys 20 25 30

Asn Ala Lys Gin Phe His Gly Pro Lys Lys Val Leu lie lie Gly Ala 35 40 45

Ser Ser Gly Phe Gly Leu Ala Thr Arg lie Ser Leu Ala Phe Gly Gly 50 55 60

Ala Lys Ala Asp Thr lie Gly Val Ser Phe Glu Thr Gly lie Thr Asp 65 70 75 80

Arg Arg Thr Gly Thr Ala Gly Trp Tyr Asn Asn lie Phe Phe Lys Glu 85 90 95

Phe Ala Glu Lys Glu Gly Leu lie Ala Lys Asn Phe lie Gly Asp Ala 100 105 110

Phe Ser Asp Glu Val Lys Glu Asn Val lie Lys Tyr lie Lys Asn Glu 115 120 125 145

Phe Gly Lys lie Asp 130

Leu Leu lie Tyr Ser Leu Ala Ser Pro Arg Arg 135 140

Lys Asp Pro Lys Thr 145

Gly Asn lie Tyr Asp Ser Thr Leu Lys Thr Thr 150 155 160

Ser Gly Glu Phe Gin 165

Gly Pro Thr lie Asp Met Glu Thr Asp Glu Leu 170 175

Val Thr Thr Lys Val 180

Asn Ser Ala Thr Asp Lys Glu lie Glu Ala Thr 185 190

Lys Lys Val Met Gly 195

Gly Glu Asp Trp Ser Glu Trp Cys Lys Leu Leu 200 205

Leu Glu Asn Asp Cys 210

Leu Ser Asp Lys Ala lie Thr lie Ser Tyr Ser 215 220

Tyr lie Gly Ala Ser 225

Arg Thr Tyr Lys lie Tyr Arg Glu Gly Thr lie 230 235 240

Gly Glu Ala Lys Arg 245

His Leu Glu Asn Thr Ala lie Gin lie Asp Lys 250 255

Glu Trp Gin Lys Lys 260 lie Asn Gly Lys Ala Phe Val Ser Val Asn Lys 265 270

Ala lie Val Thr Lys 275

Ala Ser Ala Tyr lie Pro Ser Phe Ser Leu Tyr 280 285

Ala Ala Val Leu Tyr 290

Lys Val Met Lys Glu Lys Asn Leu His Glu Asn 295 300

Cys lie Met Gin Met 305

Gin Arg Met Phe Ala Asp Lys lie Tyr Ala Glu 310 315 320

Asn Leu Leu Glu Phe 325

Asp Asp Ser Gly Arg Leu Arg Met Asp Asp Trp 330 335

Glu Leu Arg Glu Asp 340

Val Gin Ser Glu Val Asn Glu Leu Trp Glu Lys 345 350 lie Thr Pro Asp Asn 355

Phe Lys lie Leu Ser Asp Tyr Asp Gly Tyr Lys 360 365

Lys Glu Phe Met Gin 370

Leu Asn Gly Phe Glu lie Asp Gly Val Asn Tyr 375 380

Ser Glu Asp lie Asp 385 lie Glu Ala Leu Lys Arg Leu Glu Pro Glu Thr 390 395 400 <210> 97 <211> 1238 <212> DNA <213> Artificial 146 <220> 201033370 <223> Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240), Trans-2-enoyl-CoA reductase; EC=1.3.1.44 (A0KLI7) <400> 97 ggtaccggat ccctaacagg aggaattaat catgatcatc aagccgaaag ttcgtggctt 60 catctgcacc actactcacc cggttggttg cgaagctaac gttcgtcgtc agatcgctta 120 caccaaagcg aaaggtacta tcgaaaacgg tccgaagaaa gttctggtta tcggtgcttc 180 taccggttac ggtctggctt ctcgcattgc tgctgcattc ggttctggtg cggcaactct 240 gggcgtattc ttcgaaaaag cgggttctga aaccaaaact gcaactgctg gctggtacaa 300 ctccgcagca ttcgacaaag cagctaaaga agcgggtctg tacgctaaat ctatcaacgg 360 tgacgcattc tctaacgaat gccgcgctaa agttatcgaa ctgatcaagc aggacctggg 420 tcagatcgac ctggttgttt actctctggc ttctccggta cgtaagctgc cggataccgg 480 tgaagttgtt cgttctgcac tgaaaccgat cggtgaagtt tacaccacta ctgctatcga 540 caccaacaaa Gaccagatca tcactgcaac cgttgagccg gc Taacgaag aagaaatcca 600

gaacaccatc accgtaatgg gtggtcagga ctgggaactg tggstggctg cgctgcgcga 660 tgctggcgta ctggcagacg gtgcgaagtc tgttgcttac tcttacatcg gtactgacct 720 gacctggcca atctactggc acggtactct gggtcgtgcg aaagaagatt tggaccgcgc 780 tgcagctgct atccgcggtg acctggcagg caaaggtggt actgcgcacg ttgctgtact 840 gaaatctgtt gttactcagg cttcttctgc gattccggta atgccgctgt acatctctat 900 ggcattcaag attatgaaag agaaaggtat ccacgaaggt tgcatggaac aggttgaccg 960 tatgatgcgt actcgtctgt acgctgcaga catggcgctg gatgaccagg cgcgtatccg 1020 tatggacgac Tgggaactgc gtgaagatgt tcagcagact tgccgcgacc tgtggccgtc 1080 tatcacttct gaaaacctgt gcgaactgac tgactacacc ggttacaagc aggagttcct 1140 gcgtctgttc ggtttcggtc tggaagaagt tgactacgac gctgacgtta acccggacgt 1200 taagttcgac gttgttgaac tagagctcta ataagctt 1238 <210> 98

<211> 399 <212> PRT <213> Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240), Trans-2-enoyl-CoA reductase; EC=1.3.1.44 (A0KLI7) <400>

Met lie lie Lys Pro Lys Val Arg Gly Phe lie Cys Thr Thr Thr His 15 10 15

Pro Val Gly Cys Glu Ala Asn Val Arg Arg Gin lie Ala Tyr Thr Lys 20 25 30

Ala Lys Gly Thr lie Glu Asn Gly Pro Lys Lys Val Leu Val lie Gly 35 40 45

Ala Ser Thr Gly Tyr Gly Leu Ala Ser Arg lie Ala Ala Ala Phe Gly 50 55 60

Ser Gly Ala Ala Thr Leu Gly Val Phe Phe Glu Lys Ala Gly Ser Glu 65 70 75 80 147

Thr Lys Thr Ala Thr Ala Gly Trp Tyr Asn Ser Ala Ala Phe Asp Lys 85 90 95 Ala Ala Lys Glu Ala 100 Gly Leu Tyr Ala Lys Ser lie Asn Gly Asp Ala 105 110

Phe Ser Asn Glu Cys Arg Ala Lys Val lie Glu Leu lie Lys Gin Asp 115 120 125 Leu Gly Gin lie Asp 130 Leu Val Val Tyr Ser Leu Ala Ser Pro Val Arg 135 140 Lys Leu Pro Asp Thr 145 Gly Glu Val Val Arg Ser Ala Leu Lys Pro lie 150 155 160 Gly Glu Val Tyr Thr 165 Thr Thr Ala lie Asp Thr Asn Lys Asp Gin lie 170 175 He Thr Ala Thr Val 180 Glu Pro Ala Asn Glu Glu Glu Gin Asn Thr 185 190 lie Thr Val Met Gly 195 Gly Gin Asp Trp Glu Leu Trp Met Ala Ala Leu 200 205 Arg Asp Ala Gly Val 210 Leu Ala Asp Gly Ala Lys Ser Val Ala Tyr Ser 215 220 Tyr lie Gly Thr Asp 225 Leu Thr Trp Pro lie Tyr Trp His Gly Thr Leu 230 235 240 Gly Arg Ala Lys Glu 245 Asp Leu Asp Arg Ala Ala Ala Ala lie Arg Gly 250 255 Asp Leu Ala Gly Lys 260 Gly Gly Thr Ala His Val Ala Val Leu Lys Ser 265 270 Val Val Thr Gin Ala 275 Ser Ser Ala lie Pro Val Met Pro Leu Tyr lie 280 285 Ser Met Ala Phe Lys 290 lie Met Lys Glu Lys Gly lie His Glu Gly Cys 295 300 Met Glu Gin Val Asp 305 Arg Met Met Arg Thr Arg Leu Tyr Ala Ala Asp 3 10 315 320 Met Ala Leu Asp Asp 325 Gin Ala Arg lie Arg Met Asp Asp Trp Glu Leu 330 335

Arg Glu Asp Val Gin Gin Thr Cys Arg Asp Leu Trp Pro Ser lie Thr 340 345 350 Ser Glu Asn Leu Cys 355 Glu Leu Thr Asp Tyr Thr Gly Tyr Lys Gin Glu 360 365 Phe Leu Arg Leu Phe 370 Gly Phe Gly Leu Glu Glu Val Asp Tyr Asp Ala 375 380 148

Asp Val Asn Pro Asp Val Lys Phe Asp Val Val Glu Leu Glu Thr 385 390 395 <210> 99 <211> 1142 <212> DNA <213> Artificial <220><223> Yarrowia lipolytica, Probable trans-2-enoyl-CoA reductase, mitochondrial; EC = 1.3.1.38, (Q6CBE4) < aaccgaaaga 120 cgttattcag gtactggaat acccaatcga aaaaccgctg gaaaaccagg ttctgctgaa 180 atctctgggc ttcactatca acccggctga catcaaccag 99 ggtaccggat ccctaacagg aggaattaac catggccggc ttcggtactc cgtctttcgg 60 tctgcgcttc aactccgttg gtcgtgcatt cgtattctct cagactggcg; 400 & gt ctggaaggtg tttacccgtc 240 tgtaccgccg aaatctgtac agatcaacaa cgaagatgct gctatcggtg gtaacgaagg 300 tctgttccag gttctggacc cgggtgctaa atctggtctg aagaaaggcg actgggtact 360 gccgcgtaaa acttgcttcg gcacctggcg ttctcacgcg ctggttgaag ctgataccgt 420 tgttaagatc gacaacaccg acctgaccaa agttcaggca actaccgttt ctgttaaccc 480 gtctactgct tacgaaatgc tgaaagacct gaaagaaggt gactggttca tccagaacgg 540 tggtaactcc ggtgttggtc gtgctgcgat tcagatcggt cacatccgcg gtctgaaatc 600 tatctccgtt gttcgtgacc g tccggatct ggaagttctg aagaaagaac tgactgacct 660 gggtgcaact cacgttatca ctgaagaaga agcgtctgac aaactgttct ccaagcagat 720 caaatcctgg actggcggta aaatcaagct ggcactgaac tgcatcggtg gtaaatctgc 780 aacttccatc atgcgtcagc tgggtgctgg cggttctatc gttacttacg gtggtatgtc 840 caagaagccg ctgactttcc cgactggtcc gttcatcttc aaagacatca ctgcgaaagg 900 ttactggctg actcgctggg ctgacaagca cccggaagaa aaagcgaaaa ctatcgaaaa 960 catcttcaaa ttctaccgcg agaagaaatt cgttgctccg ccagttaaca tctccactct 1020 ggacttctct aaaggtaacg acgttgttct Gtctgagttc ctggatgcac tgggtaaagc 1080 acagaaaggt ggtggtaaga agcagctggt acagtgggtt gaatacgagc tctaataagc 1140 tt 1142 <210> 100 <211> 367

<212> PRT <213> Yarrowia lipolytica, Probable trans-2-enoyl-CoA reductase, mitochondrial; EC=1.3.1.38, (Q6CBE4) <400>

Met Ala Gly Phe Gly Thr Pro Ser Phe Gly Leu Arg Phe Asn Ser Val 15 10 15

Gly Arg Ala Phe Val Phe Ser Gin Thr Gly Glu Pro Lys Asp Val lie 20 25 30

Gin Val Leu Glu Tyr Pro lie Glu Lys Pro Leu Glu Asn Gin Val Leu 35 40 45 149 201033370

Leu Lys Ser Leu Gly 50 Phe Thr lie Asn Pro Ala Asp lie Asn Gin Leu 55 60 Glu Gly Val Tyr Pro 65 Ser Val Pro Pro Lys Ser Val Gin lie Asn Asn 70 75 80 Glu Asp Ala Ala lie 85 Gly Gly Asn Glu Gly Leu Phe Gin Val Leu Asp 90 95 Pro Gly Ala Lys Ser 100 Gly Leu Lys Lys Gly Asp Trp Val Leu Pro Arg 105 110 Lys Thr Cys Phe Gly 115 Thr Trp Arg Ser His Ala Leu Val Glu Ala Asp 120 125 Thr Val Val Lys Lie 130 Asp Asn Thr Asp Leu Thr Lys Val Gin Ala Thr 135 140 m ^ Thr Val Ser Val Asn 145 Pro Ser Thr Ala Tyr Glu Met Leu Lys Asp Leu 150 155 160 Lys Glu Gly Asp Trp 165 Phe lie Gin Asn Gly Gly Asn Ser Gly Val Gly 170 175 Arg Ala Ala lie Gin — 180 lie Gly His lie Arg Gly Leu Lys Ser lie Ser 185 190 Val Val Arg Asp Arg 195 Pro Asp Leu Glu Val Leu Lys Lys Glu Leu Thr 200 205 Asp Leu Gly Ala Thr 210 His Val lie Thr Glu Glu Glu Ala Ser Asp Lys 215 220 Leu Phe Ser Lys Gin 225 lie Lys Ser Trp Thr Gly Gly Lys lie Lys Leu 230 235 240 Ala Leu Asn Cys lie 245 Gly Gly Lys Ser Ala Thr Ser lie Met Arg Gi n 250 255 Leu Gly Ala Gly Gly 260 Ser lie Val Thr Tyr Gly Gly Met Ser Lys Lys 265 270 Pro Leu Thr Phe Pro 275 Thr Gly Pro Phe lie Phe Lys Asp lie Thr Ala 280 285 Lys Gly Tyr Trp Leu 290 Thr Arg Trp Ala Asp Lys His Pro Glu Glu Lys 295 300 Ala Lys Thr lie Glu 305 Asn lie Phe Lys Phe Tyr Arg Glu Lys Lys Phe 310 315 320 Val Ala Pro Pro Val 325 Asn lie Ser Thr Leu Asp Phe Ser Lys Gly Asn 330 335 Asp Val Val Leu Ser 340 Glu Phe Leu Asp Ala Leu Gly Lys Ala Gin Lys 345 350 150

Gly Gly Gly Lys Lys Gin Leu Val Gin Trp Val Glu Tyr Glu Thr 355 360 365 <210> 101 <211> 1856 <212> DNA <213> Artificial <220><223> Clostridiumkluyveri (strainATCC 8527 / DSM 555 / NCIMB 10680) (A5N5C9) < 400 > 101 ggtaccggat ccctaacagg aggaattaac catggacctg gcagaataca aaggcgtatg 60 ggtattcgct gaacagcgtg acggtgaact gcagaaagtt gcgctgcagc tggttggtaa 120 aggtcgtgaa ctggctgaca ctctgggtgt tgaactgact gcggtactgc tgggttctga 180 agttgatgac ctggcgaaag aactggttgc ttacggcgca gacaacgtac tgtacgcaga 240 ctctccgctg ctgaagcact acaccactga cggttacacc aaagttatcg acgaactgat 300 caaagagcgt aagccggaaa tcctgctgat cggtgcgact ttcatcggtc gtgacctggg 360 tccgcgcgtt gctggtcgcg tattcaccgg tctgactgca gactgcaccg gtctggatat 420 cgacgaagcg accaagaacc tgatgatgac tcgtccggca ttcggtggta acctgatggc 480 aactatcgct tgcgaaaaaa ctcgtccgca gatgtccacc gttcgtccgg gcgtattcaa 540 cgcactgccg cgcgacgctt cccgtactgg aaaatcgctg ctaacgttgc 600 taaagacgac atccgtatcg aagttctgga agttgttaag tc taagatcgaa tgctggcg acaccatcga 660 catctctgaa gcagacgtta tcgtttctgg tggtcgcggt ctgggtggtc cggacggctt 720 caaagttctg aaagaactgg ctgatctgct gggtggtact atcggtggtt ctcgcgctac 780 catcgacgct ggctggatcg acaagtctta ccaggttggt cagactggta aaaccgttcg 840 tccgggtctg tacatcgctt gcggtatctc cggtcagatt cagcacctgg caggcatgca 900 ggattccggc ttcatcgttg ctatcaacaa agacgaaaac gcaccgatga tgcaagttgc 960 tgatctggcg attgttggtg acctgtacaa agttgttcca gagttcgttg aacaggttaa 1020 agcgctgaac ttgtaataac tagtctaaca ggaggaatta atcatgaaga tcgttgtttg 1080 cctgaaacag gttccggata ccactgaagt taagatcgac ccgaaaaccg gtactctgat 1140 ccgtgaaggc gtaccgtcta tcatcaaccc ggatgacaaa aacgcgctgg aagaatccat 1200 tgctctgaaa gagaaagttg gcggcaccgt aactgttgtt tctatgggtc cgccacaggc 1260 tgttgatgcg ctgcgtgaag cgctggcaat gggtgctgac gaagcaatcc tggtatctga 1320 ccgcgctttc gctggtgctg acactcaggc aacttcttac gcactggcag gcgcactgaa 1380 aaacctggaa tacgacctga ttttcgctgg tcgtcaggct atcgacggtg acactgcaca 1440 ggttggtccg cagatcgctg aaaaactggg tatcccgcag atcacttacg ttg aaaaagt 1500 tgacgttgac ggtgacactc tgaccgtaca gcgtgcatgg gaagatggtt acgaagttgc 1560 gaaaatcaaa actccgtgca tgctgactgc tatcaaagag ctgaaccagc cgcgctacat 1620 gaacatgaag aacatcttcg aagtattcaa gaaagaagtt aaaatctggt ctgctgacga 1680 cctggacgtt gacaaaaaca agctgggtct gaacggttcc tgcaccaaag ttaagcgttc 1740 tcacaccaaa gaagcgaaag gtgctggcga aatcgttaac aagccgatca aagaagcggt 1800 151 1856 tgcttactcc atctccaagc tgcgtgaaaa acacgttatc gagctctaat aagctt < 210 > 102 <211> 334

<212> PRT <213> Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) (A5N5C9) <400>

Met Asp Leu Ala Glu Tyr Lys Gly Val Trp Val Phe Ala Glu Gin Arg 1 5 10 15 Asp Gly Glu Leu Gin Lys Val Ala Leu Gin Leu Val Gly Lys Gly Arg 20 25 30

Glu Leu Ala Asp Thr Leu Gly Val Glu Leu Thr Ala Val Leu Leu Gly 35 40 45

Ser Glu Val Asp Asp Leu Ala Lys Glu Leu Val Ala Tyr Gly Ala Asp 50 55 60 Asn Val Leu 65 Tyr Ala Asp Ser Pro Leu Leu Lys His Tyr Thr Thr Asp 70 75 80 Gly Tyr Thr Lys Val lie Asp Glu Leu lie Lys Glu Arg Lys Pro Glu 85 90 95 lie Leu Leu lie Gly Ala Thr Phe lie Gly Arg Asp Leu Gly Pro Arg 100 105 110

Val Ala Gly Arg Val Phe Thr Gly Leu Thr Ala Asp Cys Thr Gly Leu 115 120 125 Asp lie Asp 130 Glu Ala Thr Lys Asn Leu Met Met Thr Arg Pro Ala Phe 135 140 Gly Gly Asn 145 Leu Met Ala Thr lie Ala Cys Glu Lys Thr Arg Pro Gin 150 155 160 Met Ser Thr Val Arg Pro Gly Val Phe Asn Ala Leu Pro Arg Asp Ala 165 170 175 Ser Arg Thr Gly Lys lie Glu Lys lie Ala Ala Asn Val Ala Lys Asp 180 185 190 Asp lie Arg 195 Lie Glu Val Leu Glu Val Val Lys Ser Ala Gly Asp Thr 200 205 lie Asp lie 210 Ser Glu Ala Asp Val lie Val Ser Gly Gly Arg Gly Leu 215 220 Gly Gly Pro 225 Asp Gly Phe Lys Val Leu Lys Glu Leu Ala Asp Leu Leu 230 235 240

Gly Gly Thr lie Gly Gly Ser Arg Ala Thr lie Asp Ala Gly Trp lie 245 250 255

Asp Lys Ser Tyr Gin Val Gly Gin Thr Gly Lys Thr Val Arg Pro Gly 152 260 265 270

Leu Tyr lie Ala Cys Gly lie Ser Gly Gin lie Gin His Leu Ala Gly 275 280 285

Met Gin Asp Ser Gly Phe lie Val Ala lie Asn Lys Asp Glu Asn Ala 290 295 300

Pro Met Met Gin Val Ala Asp Leu Ala lie Val Gly Asp Leu Tyr Lys 305 310 315 320

Val Val Pro Glu Phe Val Glu Gin Val Lys Ala Leu Asn Leu 325 330 <210> 103 <211> 259

<212> PRT <213> Clostridiumkluyveri (strainATCC 8527 / DSM 555 / NCIMB 10680) (A5N5D0) <400> 103

Met Lys lie Val Val Cys Leu Lys Gin Val Pro Asp Thr Thr Glu Val 15 10 15

Lys lie Asp Pro Lys Thr Gly Thr Leu lie Arg Glu Gly Val Pro Ser 20 25 30 lie lie Asn Pro Asp Asp Lys Asn Ala Leu Glu Glu Ser lie Ala Leu 35 40 45

Lys Glu Lys Val Gly Gly Thr Val Thr Val Val Ser Met Gly Pro Pro 50 55 60

Gin Ala Val Asp Ala Leu Arg Glu Ala Leu Ala Met Gly Ala Asp Glu 65 70 75 80

Ala lie Leu Val Ser Asp Arg Ala Phe Ala Gly Ala Asp Thr Gin Ala 85 90 95

Thr Ser Tyr Ala Leu Ala Gly Ala Leu Lys Asn Leu Glu Tyr Asp Leu 100 105 110 lie Phe Ala Gly Arg Gin Ala lie Asp Gly Asp Thr Ala Gin Val Gly 115 120 125

Pro Gin lie Ala Glu Lys Leu Gly lie Pro Gin lie Thr Tyr Val Glu 130 135 140

Lys Val Asp Val Asp Gly Asp Thr Leu Thr Val Gin Arg Ala Trp Glu 145 150 155 160

Asp Gly Tyr Glu Val Ala Lys lie Lys Thr Pro Cys Met Leu Thr Ala 165 170 175 lie Lys Glu Leu Asn Gin Pro Arg Tyr Met Asn Met Lys Asn lie Phe 180 185 190

Glu Val Phe Lys Lys Glu Val Lys lie Trp Ser Ala Asp Asp Leu Asp 153 195 200 205

Val Asp Lys 210

Arg Ser His 225

Pro lie Lys

Asn Lys Leu Gly Leu Asn Gly Ser Cys 215 220 Thr Lys Glu Ala Lys Gly Ala Gly Glu 230 235 Glu Ala Val Ala Tyr Ser lie Ser Lys 245 250

Thr Lys Val worker le Val Asn

Leu Arg Glu 255

Lys Lys 240 Lys

His Val lie <210> 104 <211> 1196 <212> DNA <213> Artificial <220><223> Megasphaera elsdenii, Acyl-CoA dehydrogenase, short-chain specific; EC=1.3.99.2 ( Q063X9) < 400 > 104 ggtaccggat ccctaacagg aggaattaac catggacttc aacctgactg atatccagca 60 ggacttcctg aaactggcac acgacttcgg tgaaaaaaaa ctggcaccaa ccgtaactga 120 acgtgaccac aaaggtatct acgacaaaga gctgatcgac gaactgctgt ctctgggtat 180 cactggcgct tacttcgaag agaaatacgg tggttctggt gacgacggtg gtgacgttct 240 gtcttacatc ctggctgttg aagaactggc gaaatacgac gctggcgttg ctatcactct 300 gtctgcaacc gtttctctgt gcgcaaaccc gatctggcag ttcggtactg aagcgcagaa 360 agagaagttc ctggttccgc tggttgaagg tactaaactg ggtgcattcg gtctgactga 420 accgaacgct ggtactgacg cttctggtca gcagactatc gctaccaaaa acgacgacgg 480 tacttacact ctgaacggtt ctaaaatctt catcactaac ggtggtgcag ctgacatcta 540 catcgttttc gcaatgactg acaagtctaa aggtaaccac ggtatcactg cattcatcct 600 ggaagatggt actccgggct tcacttacgg taagaaagaa gataaaatgg gtatccacac 660 ttctcagact atgga actgg tattccagga cgttaaagtt ccggcagaaa acatgctggg 720 tgaagaaggt aaaggcttca aaatcgcgat gatgactctg gacggtggtc gtatcggtgt 780 tgctgctcag gcgctgggta tcgctgaagc ggcactggct gacgcggtag aatactccaa 840 gcagcgtgta cagttcggta agccgctgtg caaattccag tctatctcct tcaaactggc 900 tgatatgaag atgcagatcg aagcagctcg caacctggtt tacaaagcgg catgtaagaa 960 gcaggaaggt aagccgttca ccgttgatgc tgctatcgct aaacgcgttg cttctgacgt 1020 tgcgatgcgc gtaactactg aagctgttca gattttcggt ggttacggtt actctgaaga 1080 atacccggtt gcgcgtcaca Tgcgtgacgc taaaatcact cagatttacg aaggtactaa 1140 cgaagttcag ctgatggtta ctggcggtgc gctgctgcgt gagctctaat aagctt 1196 <210> 105 <211> 385

<212> PRT <213> Megasphaera elsdenii, Acyl-CoA dehydrogenase, short-chain specific; EC=1.3.99.2 (Q06319) 154 <400> 105

Met Asp Phe Asn Leu Thr Asp He Gin Gin Asp Phe Leu Lys Leu Ala 15 10 15

His Asp Phe Gly Glu Lys Lys Leu Ala Pro Thr Val Thr Glu Arg Asp 20 25 30

His Lys Gly lie Tyr Asp Lys Glu Leu lie Asp Glu Leu Leu Ser Leu 35 40 45

Gly lie Thr Gly Ala Tyr Phe Glu Glu Lys Tyr Gly Gly Ser Gly Asp 50 55 60

Asp Gly Gly Asp Val Leu Ser Tyr lie Leu Ala Val Glu Glu Leu Ala 65 70 75 80

Lys Tyr Asp Ala Gly Val Ala lie Thr Leu Ser Ala Thr Val Ser Leu 85 90 95

Cys Ala Asn Pro lie Trp Gin Phe Gly Thr Glu Ala Gin Lys Glu Lys 100 105 110

Phe Leu Val Pro Leu Val Glu Gly Thr Lys Leu Gly Ala Phe Gly Leu 115 120 125

Thr Glu Pro Asn Ala Gly Thr Asp Ala Ser Gly Gin Gin Thr lie Ala 130 135 140

Thr Lys Asn Asp Asp Gly Thr Tyr Thr Leu Asn Gly Ser Lys lie Phe 145 150 155 160 lie Thr Asn Gly Gly Ala Ala Asp lie Tyr lie Val Phe Ala Met Thr 165 170 175

Asp Lys Ser Lys Gly Asn His Gly lie Thr Ala Phe lie Leu Glu Asp 180 185 190

Gly Thr Pro Gly Phe Thr Tyr Gly Lys Lys Glu Asp Lys Met Gly lie 195 200 205

His Thr Ser Gin Thr Met Glu Leu Val Phe Gin Asp Val Lys Val Pro 210 215 220

Ala Glu Asn Met Leu Gly Glu Glu Gly Lys Gly Phe Lys lie Ala Met 225 230 235 240

Met Thr Leu Asp Gly Gly Arg lie Gly Val Ala Ala Gin Ala Leu Gly 245 250 255 lie Ala Glu Ala Ala Leu Ala Asp Ala Val Glu Tyr Ser Lys Gin Arg 260 265 270

Val Gin Phe Gly Lys Pro Leu Cys Lys Phe Gin Ser lie Ser Phe Lys 275 280 285

Leu Ala Asp Met Lys Met Gin lie Glu Ala Ala Arg Asn Leu Val Tyr 155 290 295 300

Lys Ala Ala Cys Lys Lys Gin Glu Gly Lys Pro Phe Thr Val Asp Ala 305 310 315 320

Ala lie Ala Lys Arg Val Ala Ser Asp Val Ala Met Arg Val Thr Thr 325 330 335

Glu Ala Val Gin lie Phe Gly Gly Tyr Gly Tyr Ser Glu Glu Tyr Pro 340 345 350

Val Ala Arg His Met Arg Asp Ala Lys lie Thr Gin lie Tyr Glu Gly 355 360 365

Thr Asn Glu Val Gin Leu Met Val Thr Gly Gly Ala Leu Leu Arg Glu 370 375 380

Thr 385 <210> 106 <211> 1421 <212> DNA <213> Artificial <220><223> Acinetobacter sp. (strain ADP1), Acyl-CoA dehydrogenase; EC=1.3.99 (Q937T2 ) < 400 > 106 ggtacctttt ttgggctaac aggaggaatt aaccatgatc aacaagatca tcaacgatat 60 cgaaccgatt ctgaaatcta tcccagacgg ttctaccatc atgacttctg gcttcggtac 120 taccggtcag ccggaagcgc tgctggaagc gctgatcgac ttcgcaccga aagagctgac 180 tatcatcaac aacaacgctt cttccggtcc gaacggtctg actcagctgt tcactgctgg 240 tctggttaag aaactgatct gctcctaccc gaaatctatc tcttccaccg ttttcccgga 300 cctgtaccgc gctggtaaga tcgaactgga actggttccg cagggtaacc tggcttgccg 360 tatccaggca gctggcgcgg gtctgggtgc agtattcact ccgactggtt acggtactaa 420 gatcgctgaa ggtaaagaaa ctcgtatcat caacggtaaa aactacgttc tggaataccc 480 gctggaagct gactacgcat tcatctacgc tgacaaagct gaccgctggg gtaacctgac 540 ttaccgtaaa gcggcacgta acttcggtcc gatcatggcg aaagcagcta aaaccactat 600 tgctcaggtt aaccagactg ttgaactggg cgatctggac ccggaatgca tcatcactcc 660 gggtatcttc gtacagcacg ttgttcgtct gggtgacatc aaataatttt ttgggctaac 720 aggaggaatt aaccatgact attcagaagc gttctcgcga agatatcgct atcatgatcg 780 ctaaagacat cccggacggt tcttacgtta acctgggtat cggtctgccg actcacgttg 840 ctaaatatct gccgaaagac aaagaaatct tcctgcactc tgaaaacggt gttctggcat 900 tcggtccgcc gccagcagaa ggtgaagaag atcaggacct ggttaacgct ggtaaagagc 960 tggtaactct gctgtctggt ggttgcttca tgcaccacgg tgactccttc gacatcatgc 1020 gtggtggtca cctggatatc tgcgttatcg gtgcattcca ggttgcgctg aacggtgacc 1080 tggctaactg gcacaccggt aaagacgacg acgttccggc agtaggtggt gcaatggacc 1140 156 201033370 tggctgttgg tgcgaagcgt atcttcgttt acatggaaca caccaccaag aaaggtgaac 1200 cgaaaatcgt taagcacctg acttacccga tcaccggtga acagtgcgta gaccgtatct 1260 acactgacct gtgcaccatc gaactgaaag acggtcaggc ttacgttatc gaaatggttg 1320 acggtctgga tttcgacact ctgcaggcgc tgactgaatg cccgctgatc gaccactgca 1380 cctactcttc tctgattcag ctcagataag gatccgagct c 1421 < 210 > 107 < 211 > 223

<212> PRT <213> Acinetobacter sp. (strain ADP1), Acyl-CoA dehydrogenase; EC=1.3.99 (Q937T2) <400>

Met lie Asn Lys lie lie Asn Asp lie Glu Pro lie Leu Lys Ser lie 15 10 15

Pro Asp Gly Ser Thr lie Met Thr Ser Gly Phe Gly Thr Thr Gly Gin 20 25 30

Pro Glu Ala Leu Leu Glu Ala Leu lie Asp Phe Ala Pro Lys Glu Leu 35 40 45

Thr lie lie Asn Asn Asn Ala Ser Ser Gly Pro Asn Gly Leu Thr Gin 50 55 60

Leu Phe Thr Ala Gly Leu Val Lys Lys Leu lie Cys Ser Tyr Pro Lys 65 70 75 80

Ser lie Ser Ser Thr Val Phe Pro Asp Leu Tyr Arg Ala Gly Lys lie 85 90 95

Glu Leu Glu Leu Val Pro Gin Gly Asn Leu Ala Cys Arg lie Gin Ala 100 105 110

Ala Gly Ala Gly Leu Gly Ala Val Phe Thr Pro Thr Gly Tyr Gly Thr 115 120 125

Lys lie Ala Glu Gly Lys Glu Thr Arg lie lie Asn Gly Lys Asn Tyr 130 135 140

Val Leu Glu Tyr Pro Leu Glu Ala Asp Tyr Ala Phe lie Tyr Ala Asp 145 150 155 160

Lys Ala Asp Arg Trp Gly Asn Leu Thr Tyr Arg Lys Ala Ala Arg Asn 165 170 175

Phe Gly Pro lie Met Ala Lys Ala Ala Lys Thr Thr lie Ala Gin Val 180 185 190

Asn Gin Thr Val Glu Leu Gly Asp Leu Asp Pro Glu Cys lie lie Thr 195 200 205

Pro Gly lie Phe Val Gin His Val Val Arg Leu Gly Asp lie Lys 210 215 220 157 <210> 108 <211> 1190 <212> DNA <213> Artificial <220><223> Deinococcus radiodurans, Acyl-CoA dehydrogenase (Q9RW0) < 400 > 108 ggtaccggat ccctaacagg aggaattaac catggacttc aatctgccgg acgatctgcg 60 tgaagttcag gcaactatcc gtgacttcat gctgactcgc gttgaagatc gcgctcagga 120 aatcgaacac accaactccg taccgccgga actgatcaaa gaagcggcag acctgggtct 180 gttcggtctg tctatcccgg aagaatacgg tggtgttggt ctgtcctctc tgggtcgttg 240 cgcagtttac gaagcgatgg gtcagggtca catgggcttc ggtggtatga tctccgcaca 300 cgcttctatc ggtacttctg gtctggttaa actgggtaac gaagagcaga agcagcgttt 360 cctgccgcgt atggcggcag gcgaatgcat cgctggcttc gctatcactg aaccaagctc 420 cggttctgac gctggtaaca tccgtactaa agctgttaag aaaggcgacg tttacgttct 480 gaacggtact aagcactaca tctccaacgc tccgatcgct ggtctgctga ccgttatcgc 540 tatcactgac ccggcacagg gttcccgtgg tatgtccgca ttcctggttg aaccgcagtc 600 tactccgggc gtatctatcg gtaaaatcga cgaaaaaatg ggtcagaaag gt gcgctgtc 660 tgcagaagtt atcttcgaag atgctgaaat cccggctgct aaccttctcg gtccggaaaa 720 ccgcggttac cgtgaagcgc tgggtattct gactaacggt cgcgttggta tcgctgcacg 780 ttctactggc gcgatgcagc gtctgctgga cctgtctgtt gcacacgctc agactcgcga 840 gcagttcggt aagccgatcg ctgagttcca ggcggtacag ttcatgctgg cagaaatgga 900 agttgcgatt cagacttctc gcgtactgtg gcagaaagtt gcatggatgg ttgaccaggg 960 tcaggacgtt aagcgtatgg cgtctgttgc taaataccac gcaactgaaa tgctgtctca 1020 ggttgctgac aaagcggtac aggttgctgg cggtatgggt tacgttaaag acgctccgtt 1080 cgaacgtttc taccgcgacc agcgtctgct gcgtatctac gaaggtactt ctgaaatcca 1140 gaaagttatc atcgctgctg aactgctgcg tcgcgagctc taataagctt 1190 <210> 109

<211> 383 <212> PRT <213> Deinococcus radiodurans, Acyl-CoA dehydrogenase (Q9RW0) <400> 109

Met Asp Phe Asn Leu Pro Asp Asp Leu Arg Glu Val Gin Ala Thr lie 15 10 15

Arg Asp Phe Met Leu Thr Arg Val Glu Asp Arg Ala Gin Glu lie Glu 20 25 30

His Thr Asn Ser Val Pro Pro Glu Leu lie Lys Glu Ala Ala Asp Leu 35 40 45

Gly Leu Phe Gly Leu Ser lie Pro Glu Glu Tyr Gly Gly Val Gly Leu 50 55 60

Ser Ser Leu Gly Arg Cys Ala Val Tyr Glu Ala Met Gly Gin Gly His 65 70 75 80 158 201033370

Met Gly Phe Gly Gly Met lie Ser Ala His Ala Ser lie Gly Thr Ser 85 90 95

Glv Leu Val Lys Leu Gly Asn Glu Glu Gin Lys Gin Arg Phe Leu Pro 100 105 110

Arg Met Ala Ala Gly Glu Cys lie Ala Gly Phe Ala lie Thr Glu Pro 115 120 125

Ser Ser Gly Ser Asp Ala Gly Asn lie Arg Thr Lys Ala Val Lys Lys 130 135 140

Gly Asp Val Tyr Val Leu Asn Gly Thr Lys His Tyr lie Ser Asn Ala 145 150 155 160

Pro lie Ala Gly Leu Leu Thr Val lie Ala lie Thr Asp Pro Ala Gin 165 170 175

Gly Ser Arg Gly Met Ser Ala Phe Leu Val Glu Pro Gin Ser Thr Pro 180 185 190

Gly Val Ser lie Gly Lys lie Asp Glu Lys Met Gly Gin Lys Gly Ala 195 200 205

Leu Ser Ala Glu Val lie Phe Glu Asp Ala Glu lie Pro Ala Ala Asn 210 215 220

Leu Leu Gly Pro Glu Asn Arg Gly Tyr Arg Glu Ala Leu Gly lie Leu 225 230 235 240

Thr Asn Gly Arg Val Gly lie Ala Ala Arg Ser Thr Gly Ala Met Gin 245 250 255

Arg Leu Leu Asp Leu Ser Val Ala His Ala Gin Thr Arg Glu Gin Phe 260 265 270

Gly Lys Pro lie Ala Glu Phe Gin Ala Val Gin Phe Met Leu Ala Glu 275 280 285

Met Glu Val Ala lie Gin Thr Ser Arg Val Leu Trp Gin Lys Val Ala 290 295 300

Trp Met Val Asp Gin Gly Gin Asp Val Lys Arg Met Ala Ser Val Ala 305 310 315 320

Lys Tyr His Ala Thr Glu Met Leu Ser Gin Val Ala Asp Lys Ala Val 325 330 335

Gin Val Ala Gly Gly Met Gly Tyr Val Lys Asp Ala Pro Phe Glu Arg 340 345 350

Phe Tyr Arg Asp Gin Arg Leu Leu Arg lie Tyr Glu Gly Thr Ser Glu 355 360 365 lie Gin Lys Val lie lie Ala Ala Glu Leu Leu Arg Arg Glu Thr 370 375 380 159 <210> 110 <211> 1202 <212> DNA <213> Artificial <220><223> Arabidopsis thaliana, Isovaleryl-CoA dehydrogenase, mitochondrial; Short=IVD; EC=1.3.99.10 <400> 110 ggtaccggat ccctaacagg aggaattaac catggcctct tctctgctgt tcgacgacac 60 tcagctgcag ttcaaagaat ccgtttccaa gttcgctcag gacaacatcg ctccgcacgc 120 tgaacgtatc gacaaaacta actccttccc gaaagacgtt aacctgtgga aactgatggg 180 cgagttcaat ctgcacggta tcactgcgcc agaagaatac ggtggtctgg gtctgggtta 240 cctgtaccac tgcatcgcga tggaagaaat ctctcgcgct tctggttctg ttgctctgtc 300 ttacggcgca cactctaacc tgtgcatcaa ccagctggta cgtaacggta ctgctgcaca 360 gaaagagaag tatctgccga aactgatctc cggtgaacac gttggtgcac tggcgatgtc 420 tgaaccgaac gctggttctg acgttgttgg tatgaagtgc aaagcagaaa aagttgacgg 480 tggttacatt ctgaacggta acaagatgtg gtgcaccaac Ggtccgtctg ctgaaactct 540 ggttgtttac gct aaaactg acaccaaagc gggttctaaa ggtatcactg cattcatcat 600 cgaaaaaggt atgaccggtt tctccactgc tcagaagctg gacaagctgg gtatgcgcgg 660 ttctgacact tgcgaactgg tattcgaaaa ctgcttcgta ccggaagaaa acatcctgga 720 taaagaaggt aaaggcgttt acgttctgat gtccggtctg gatctggaac gtctggtact 780 ggcagctggt ccgctgggta tcatgcaggc ttgcctggat aacgtactgc cgtacatccg 840 tcagcgtgaa cagttcggtc gtccggttgg cgagttccag ttcatccagg gtaaagttgc 900 tgacatgtac actgcgctgc agtcctcccg ttcttacgtt tactccgttg ctcgcgactg 960 cgacaacggt aaagttgatc cgaaagactg cgcaggcact atcctgtgcg cagctgaacg 1020 tgcaactcag gttgcgctgc aggcgattca gtgcctgggt ggtaacggtt acatcaacga 1080 atacgcaact ggtcgtctgc tgcgcgacgc gaagctgtac gaaatcggtg ctggtacttc 1140 tgaaatccgc cgtatcgtta tcggtcgtga actgttcaaa gaagaagagc tctaataagc 1200 tt 1202 < 210 > 111 < 211 > 387 < 212 > PRT < 213 > Arabidopsis thaliana, Isovaleryl-CoA mitochondrial; Short=IVD; EC=1.3.99.10 dehydrogenase, <400> 111

Met Ala Ser Ser Leu Leu Phe Asp Asp Thr Gin Leu Gin Phe Lys Glu 15 10 15

Ser Val

Ser Lys Phe 20

Ala Gin Asp Asn 25 lie Ala

Pro His Ala Glu Arg 30 lie Asp Lys Thr Asn Ser Phe 35

Pro Lys Asp Val Asn Leu Trp Lys Leu 40 45

Gly lie

Met Gly Glu Phe Asn Leu His

Thr Ala Pro Glu Glu Tyr Gly 160 201033370 50 55 60

Gly Leu Gly Leu Gly Tyr Leu Tyr His Cys lie Ala Met Glu Glu lie 65 70 75 80 Ser Arg Ala Ser Gly Ser Val Ala Leu Ser Tyr Gly Ala His 85 90 Ser Asn 95 Leu Cys lie Asn Gin Leu Val Arg Asn Gly Thr Ala Ala Gin 100 105 110 Lys Glu Lys Tyr Leu Pro Lys Leu lie Ser Gly Glu His Val Gly Ala 115 120 125 Leu Ala Met Ser Glu Pro Asn Ala Gly Ser Asp Val Val Gly Met Lys 130 135 140 Cys Lys Ala Glu Lys Val Asp Gly Gly Tyr lie Leu Asn Gly Asn Lys 145 150 155 Met Trp 160 〇Cys Thr Asn Gly Pro Ser Ala Glu Thr Leu Val Val Tyr Ala Lys Thr 165 170 175

Asp Thr Lys Ala Gly Ser Lys Gly lie Thr Ala Phe lie lie 180 185 190 Glu Lys Gly Met Thr Gly Phe Ser Thr Ala Gin Lys Leu Asp Lys Leu 195 200 205 Gly Met Arg Gly Ser Asp Thr Cys Glu Leu Val Phe Glu Asn Cys Phe 210 215 220 Val Pro Glu Glu Asn lie Leu Asp Lys Glu Gly Lys Gly Val Tyr Val 225 230 235 Leu Met 240 Ser Gly Leu Asp Leu Glu Arg Leu Val Leu Ala Ala Gly Pro 245 250 Leu Gly 255 Q lie Met Gin Ala Cys Leu Asp Asn Val Leu Pro Tyr lie Arg 260 265 270 Gin Arg

Glu Gin Phe Gly Arg Pro Val Gly Glu Phe Gin Phe lie Gin Gly Lys 275 280 285

Val Ala Asp Met Tyr Thr Ala Leu Gin Ser Ser Arg Ser Tyr Val Tyr 290 295 300

Ser Val Ala Arg Asp Cys Asp Asn Gly Lys Val Asp Pro Lys 305 310 315 Asp Cys 320 Ala Gly Thr lie Leu Cys Ala Ala Glu Arg Ala Thr Gin Val Ala Leu 325 330 335

Gin Ala lie Gin Cys Leu Gly Gly Asn Gly Tyr lie Asn Glu Tyr Ala 340 345 350 161

Thr Gly Arg Leu Leu Arg Asp Ala Lys Leu Tyr Glu lie Gly Ala Gly 355 360 365

Thr Ser Glu lie Arg Arg lie Val lie Gly Arg Glu Leu Phe Lys Glu 370 375 380

Glu Glu Thr 385 <210> 112 <211> 1232 <212> DNA <213> Artificial <220><223> Homo sapiens, Glutaryl-CoA dehydrogenase, mitochondrial; Short=GCD; EC=1.3. 99.7 (Q92947) < 400 > 112 ggtaccggat ccctaacagg aggaattaac catgggtccg gaatttgact ggcaggaccc 60 gctggtactg gaagagcagc tgaccactga cgaaatcctg atccgtgaca ccttccgtac 120 ttactgccag gaacgtctga tgccgcgcat tctgctggct aaccgtaacg aagttttcca 180 ccgtgaaatc atctctgaaa tgggtgaact gggtgttctg ggtccgacta tcaaaggtta 240 cggttgcgca ggcgtttctt ccgttgctta cggtctgctg gcgcgtgaac tggaacgcgt 300 tgactccggt taccgttctg caatgtccgt tcagtcctct ctggtaatgc acccaatcta 360 cgcttacggt tctgaagagc agcgtcagaa atatctgccg cagctggcga aaggtgaact 420 gctgggttgc ttcggtctga ctgaaccgaa ctccggttct gacccgtcct ctatggaaac 480 tcgcgcacac tacaactctt ctaacaagtc ttacactctg aacggcacca aaacctggat 540 cactaactct ccgatggctg acctgttcgt tgtatgggct cgctgcgaag atggttgcat 600 ccgcggtttc ctgctggaaa aaggtatgcg tggtctgtct gcaccgcgta tccagggtaa 660 gttctctctg c gcgcttctg caactggtat gatcatcatg gacggtgttg 720 agaaaacgtt ctgccgggtg catcttctct gggtggtccg ttcggttgcc tgaacaacgc 780 gcgttacggt atcgcatggg gcgtactggg tgcttctgag ttctgcctgc acactgcacg 840 tcagtacgca ctggatcgca tgcagttcgg tgttccgctg gcgcgtaacc agctgattca 900 gaagaaactg gctgacatgc tgactgaaat cactctgggt ctgcacgctt gcctgcagct 960 gggtcgtctg aaagaccagg acaaagctgc accggaaatg gtttctctgc tgaagcgtaa 1020 caactgcggt aaagcgctgg atatcgctcg ccaggctcgc gacatgctgg gtggtaacgg 1080 tatctctgac gaataccacg aagttccgga Tttccgtca cgcgatgaac ctggaagctg ttaacaccta 1140 cgaaggtact cacgacatcc acgcgctgat cctgggtcgt gctatcaccg gtatccaggc 1200 tttcactgct tctaaagagc tctaataagc tt 1232 <210> 113

<211> 397 <212> PRT <213> Homo sapiens, Glutaryl-CoA dehydrogenase, mitochondrial; Short=GCD; EC=1.3.99.7 (Q92947) <400> 113

Met Gly Pro Glu Phe Asp Trp Gin Asp Pro Leu Val Leu Glu Glu Gin 15 10 15 162 201033370

Leu Thr Thr Asp Glu lie Leu lie Arg Asp Thr Phe Arg Thr Tyr Cys 20 25 30

Gin Glu Arg Leu Met Pro Arg lie Leu Leu Ala Asn Arg Asn Glu Val 35 40 45

Phe His Arg Glu lie lie Ser Glu Met Gly Glu Leu Gly Val Leu Gly 50 55 60

Pro Thr lie Lys Gly Tyr Gly Cys Ala Gly Val Ser Ser Val Ala Tyr 65 70 75 80

Gly Leu Leu Ala Arg Glu Leu Glu Arg Val Asp Ser Gly Tyr Arg Ser 85 90 95

Ala Met Ser Val Gin Ser Ser Leu Val Met His Pro lie Tyr Ala Tyr 100 105 110

Gly Ser Glu Glu Gin Arg Gin Lys Tyr Leu Pro Gin Leu Ala Lys Gly 115 120 125

Glu Leu Leu Gly Cys Phe Gly Leu Thr Glu Pro Asn Ser Gly Ser Asp 130 135 140

Pro Ser Ser Met Glu Thr Arg Ala His Tyr Asn Ser Ser Asn Lys Ser 145 150 155 160

Tyr Thr Leu Asn Gly Thr Lys Thr Trp lie Thr Asn Ser Pro Met Ala 165 170 175

Asp Leu Phe Val Val Trp Ala Arg Cys Glu Asp Gly Cys lie Arg Gly 180 185 190

Phe Leu Leu Glu Lys Gly Met Arg Gly Leu Ser Ala Pro Arg lie Gin 195 200 205

Gly Lys Phe Ser Leu Arg Ala Ser Ala Thr Gly Met lie lie Met Asp 210 215 220

Gly Val Glu Val Pro Glu Glu Asn Val Leu Pro Gly Ala Ser Ser Leu 225 230 235 240

Gly Gly Pro Phe Gly Cys Leu Asn Asn Ala Arg Tyr Gly lie Ala Trp 245 250 255

Gly Val Leu Gly Ala Ser Glu Phe Cys Leu His Thr Ala Arg Gin Tyr 260 265 270

Ala Leu Asp Arg Met Gin Phe Gly Val Pro Leu Ala Arg Asn Gin Leu 275 280 285 lie Gin Lys Lys Leu Ala Asp Met Leu Thr Glu lie Thr Leu Gly Leu 290 295 300

His Ala Cys Leu Gin Leu Gly Arg Leu Lys Asp Gin Asp Lys Ala Ala 305 310 315 320 163

Pro Glu

Met Val Ser Leu Leu Lys 325

Arg As n 330

Asp lie

Ala Arg Gin Ala Arg Asp 340

Met Leu 345

Asp Glu

Tyr His Val lie Arg His 355 360

Ala Met

Thr Tyr 370

Glu Gly Thr His Asp lie 375

His Ala lie Thr 385

Gly lie Gin Ala Phe Thr 390

Ala Ser

Asn Cys Gly Gly Asn Leu Leu lie 380 Lys Glu 395

Gly Lys Ala 335

Asn Gly lie 350

Glu Ala Val 365

Leu Gly Arg

Leu Ser Asn Ala

Thr <210> 114 <211> 1784 <212> DNA <213> Artificial <220><223> Homo sapiens. Electron transfer flavoprotein subunit alpha, mitochondrial (P13804) <400> 114 ggtaccggat ccctaacagg aggaattaat catgaccact ctggttatcg ctgaacacgc 60 taacgactct ctggcaccaa tcactctgaa 120 tgaagtttcc caccatcact gcggcaactc gtctgggtgg tgcctggttg ctggcaccaa gtgcgacaaa gttgctcagg acctgtgcaa 180 agtagcgggt atcgcgaaag ttctggtagc gcagcacgac gtttacaaag gtctgctgcc 240 ggaagagctg actccgctga tcctggcaac tcagaagcag ttcaactaca ctcacatctg 300 tgcgggtgct tctgcattcg gtaaaaatct gctgccgcgc gttgctgcga aactggaagt 360 tgcaccgatt tctgacatca tcgctatcaa gtctccggat accttcgtac gtactatcta 420 cgctggtaac gcactgtgca ccgttaagtg cgacgaaaaa Gttaaagtat tctctgtacg 480 tggtacttct ttcgacgctg cagcaacttc tggtggttct gcatcttctg aaaaagcatc 540 ttccacttct ccggttgaaa tctctgagtg gctggatcag aaactgacca agtctgaccg 600 tccggaactg actggcgcga aagttgttgt ttctggtggt cgtggtctga aatctggtga 660 aaacttcaaa ctgctgtacg acctggctga ccagctgcac gctgctgttg gtgcttctcg 720 cgctgctgtt gatgctggct tcgtaccgaa cgacatgcaa gttggtcaga ctggtaaaat 780 cgttgcaccg gaactgtaca tcgctgttgg tatctccggt gcgattcagc acctggcggg 840 tatgaaagac tccaaaacta tcgttgctat caacaaagac ccggaagcac cgatcttcca 900 ggttgctgac tacggtatcg ttgctgacct gttcaaagtt gttccggaaa tgactgaaat 960 cctgaagaag aagtaataac tagtctaaca ggaggaatta accatggctg aactgcgcgt 1020 actggttgct gttaagcgcg ttatcgacta cgcagttaaa atccgcgtta agccggaccg 1080 tactggcgtt gttactgacg gtgttaaaca ctctatgaac ccgttctgcg aaatcgctgt 1140 tgaagaagcg gtacgtctga aagagaagaa actggttaaa gaagttatcg ctgtttcctg 1200 cggtccggca cagtgccagg aaaccattcg tactgcactg gcgatgggcg cagaccgcgg 1260 tatccacgtt gaagttccgc cagctgaagc agaacgtctg ggtccgctcc aggttgctcg 1320 164 cgtactggcg aaactggctg aaaaagaaaa agttgacctg gtactgctgg gtaagcaggc 1380 tatcgacgac gactgcaacc agactggtca gatgactgct ggcttcctgg actggccgca 1440 gggtactttc gcttctcagg taactctgga aggtgacaaa ctgaaagttg aacgtgaaat 1500 cgacggtggt ctggaaactc tgcgtctgaa actgcc ggca gttgttactg ctgatctgcg 1560 tctgaacgaa ccgcgttacg caactctgcc gaacattatg aaagcgaaga agaagaaaat 1620 cgaagttatc aagccgggcg acctgggtgt tgacctgact tctaaactgt ctgttatctc 1680 cgttgaagat ccgccgcagc gtactgctgg cgttaaagtt gaaaccactg aggatctggt 1740 agcgaaactg aaagaaatcg gtcgtatcga gctctaataa gctt 1784 < 210 > 115 < 211 > 314

<212> PRT <213> Homo sapiens, Electron transfer flavoprotein subunit alpha, mitochondrial (PI3804) <400>

Met Thr Thr Leu Val lie Ala Glu His Ala Asn Asp Ser Leu Ala Pro 15 10 15 lie Thr Leu Asn Thr 20 lie Thr Ala Ala Thr Arg Leu Gly Gly Glu Val 25 30

Ser Cys Leu Val Ala Gly Thr Lys Cys Asp Lys Val Ala Gin Asp Leu 35 40 45

Cys Lys Val Ala Gly lie Ala Lys Val Leu Val Ala Gin His Asp Val 50 55 60

Tyr Lys Gly Leu Leu Pro Glu Glu Leu Thr Pro Leu lie Leu Ala Thr 65 70 75 80

Gin Lys Gin Phe Asn Tyr Thr His lie Cys Ala Gly Ala Ser Ala Phe 85 90 95

Gly Lys Asn Leu Leu Pro Arg Val Ala Ala Lys Leu Glu Val Ala Pro 100 105 110 lie Ser Asp lie lie Ala lie Lys Ser Pro Asp Thr Phe Val Arg Thr 115 120 125 lie Tyr Ala Gly Asn Ala Leu Cys Thr Val Lys Cys Asp Glu Lys Val 130 135 140

Lys Val Phe Ser Val Arg Gly Thr Ser Phe Asp Ala Ala Ala Thr Ser 145 150 155 160

Gly Gly Ser Ala Ser Ser Glu Lys Ala Ser Ser Thr Val Valu 165 170 175 lie Ser Glu Trp Leu Asp Gin Lys Leu Thr Lys Ser Asp Arg Pro Glu 180 185 190

Leu Thr Gly Ala Lys Val Val Val Ser Gly Gly Arg Gly Leu Lys Ser 195 200 205 165

Gly Glu Asn Phe Lys Leu Leu Tyr Asp Leu Ala Asp Gin Leu His Ala 210 215 220

Ala Val Gly Ala Ser Arg Ala Ala Val Asp Ala Gly Phe Val Pro Asn 225 230 235 240

Asp Met Gin Val Gly Gin Thr Gly Lys lie Val Ala Pro Glu Leu Tyr 245 250 255 lie Ala Val Gly lie Ser Gly Ala lie Gin His Leu Ala Gly Met Lys 260 265 270

Asp Ser Lys Thr lie Val Ala lie Asn Lys Asp Pro Glu Ala Pro lie 275 280 285

Phe Gin Val Ala Asp Tyr Gly lie Val Ala Asp Leu Phe Lys Val Val 290 295 300

Pro Glu Met Thr Glu lie Leu Lys Lys Lys 305 310 <210> 116 <211> 255

<212> PRT <213> Homo sapiens, Electron transfer flavoprotein subunit beta (P38117) <400> 116

Met Ala Glu Leu Arg Val Leu Val Ala Val Lys Arg Val lie Asp Tyr 1 5 10 15

Ala Val Lys lie Arg Val Lys Pro Asp Arg Thr Gly Val Val Thr Asp 20 25 30

Gly Val Lys His Ser Met Asn Pro Phe Cys Glu lie Ala Val Glu Glu 35 40 45

Ala Val Arg Leu Lys Glu Lys Lys Leu Val Lys Glu Val lie Ala Val 50 55 60

Ser Cys Gly Pro Ala Gin Cys Gin Glu Thr lie Arg Thr Ala Leu Ala 65 70 75 80

Met Gly Ala Asp Arg Gly lie His Val Glu Val Pro Pro Ala Glu Ala 85 90 95

Glu Arg Leu Gly Pro Leu Gin Val Ala Arg Val Leu Ala Lys Leu Ala 100 105 no

Glu Lys Glu Lys Val Asp Leu Val Leu Leu Gly Lys Gin Ala lie Asp 115 120 125

Asp Asp Cys Asn Gin Thr Gly Gin Met Thr Ala Gly Phe Leu Asp Trp 130 135 140

Pro Gin Gly Thr Phe Ala Ser Gin Val Thr Leu Glu Gly Asp Lys Leu 145 150 155 160 166

Lys Val Glu Arg Glu lie Asp Gly Gly Leu Glu Thr Leu Arg Leu Lys 165 170 175

Leu Pro Ala Val Val Thr Ala Asp Leu Arg Leu Asn Glu Pro Arg Tyr 180 185 190

Ala Thr Leu Pro Asn Gong Le Met Lys Ala Lys Lys Lys Lys lie Glu Val 195 200 205 lie Lys Pro Gly Asp Leu Gly Val Asp Leu Thr Ser Lys Leu Ser Val 210 215 220 lie Ser Val Glu Asp Pro Pro Gin Arg Thr Ala Gly Val Lys Val Glu 225 230 235 240

Thr Thr Glu Asp Leu Val Ala Lys Leu Lys Glu lie Gly Arg lie 245 250 255 <210> 117 <211> 316

<212> PRT <213> Homo sapiens Acyl-coenzyme A thioesterase 8, EC = 3.1.2.27 (014734) <400>

Met Ser Ser Pro Gin Ala Pro Glu Asp Gly Gin Gly Cys Gly Asp Arg 1 5 10 15

Gly Asp Pro Pro Gly Asp Leu Arg Ser Val Leu Val Thr Thr Val Leu 20 25 30

Asn Leu Glu Pro Leu Asp Glu Asp Leu Phe Arg Gly Arg His Tyr Trp 35 40 45

Val Pro Ala Lys Arg Leu Phe Gly Gly Gin lie Val Gly Gin Ala Leu 50 55 60

Val Ala Ala Ala Lys Ser Val Ser Glu Asp Val His Val His Ser Leu 65 70 75 80

His Cys Tyr Phe Val Arg Ala Gly Asp Pro Lys Leu Pro Val Leu Tyr 85 90 95

Gin Val Glu Arg Thr Arg Thr Gly Ser Ser Phe Ser Val Arg Ser Val 100 105 110

Lys Ala Val Gin His Gly Lys Pro lie Phe lie Cys Gin Ala Ser Phe 115 120 125

Gin Gin Ala Gin Pro Ser Pro Met Gin His Gin Phe Ser Met Pro Thr 130 135 140

Val Pro Pro Pro Glu Glu Leu Leu Asp Cys Glu Thr Leu lie Asp Gin 145 150 155 160

Tyr Leu Arg Asp Pro Asn Leu Gin Lys Arg Tyr Pro Leu Ala Leu Asn 165 170 175 167

Tyr Glu Cys Glu Ser 275

Arg lie Ala Ala Gin Glu Val Pro lie Glu lie Lys Pro Val Asn 180 185 190

Ser Pro Leu Ser Gin Leu Gin Arg Met Glu Pro Lys Gin Met Phe 195 200 205

Val Arg Ala Arg Gly Tyr lie Gly Glu Gly Asp Met Lys Met His 210 215 220

Cys Val Ala Ala Tyr lie Ser Asp Tyr Ala Phe Leu Gly Thr Ala 225 230 235

Leu Pro His Gin Trp Gin His Lys Val His Phe Met Val Ser Leu 245 250 255

His Ser Met Trp Phe His Ala Pro Phe Arg Ala Asp His Trp Met 260 265 270

Pro Trp Ala Gly Gly Ser Arg Gly Leu Val 280 285

Gly Arg Leu Trp Arg Gin Asp Gly Val Leu Ala Val Thr Cys Ala 290 295 300

Glu Gly Val lie Arg Val Lys Pro Gin Val Ser Glu 305 310 315 <210> 118 <211> 931 <212> DNA <213> Artificial <220><223> Hyphomonas neptunium Acyl-CoA thioesterase ( Q0BZ30) < 400 > 118 ggtacctttt ttgggctaac aggaggaatt aaccatggcg aaagacccgg ttggcgatct gctgactctg ctggatctgg aacgtctgga actggatctg ttccgcggtc agtctccgga cgaagaagaa tctcagcgcg tattcggtgg tcaggttatc gctcagtctc tggttgctgc ttaccgtact gtaccggacg accgtctgtg ccactctctg cactgctact tcatccgtcc gggcgacccg tctgtaccaa tcatctacca ggttgaccac tctcgcgacg gtggttcttt caccactcgt cgcgttgttg ctatccagca cggtaagcag attttcaacc tggcagcttc tttccacgtt gttgaagatt cctggcacca ccagcacgaa atgccggaag ttgacccgcc agaatccgta ggtgaccgta tcgagtggcg tcgtaaattc gctgaacagg ttccggaacg tcaccgcggt cacttcctgc gcgaccgtcc ggttgaaatg cgtgaaatcg acccgctgga cccgctgaaa ccggcaaaag cgtctgacca gcagaacctg tggttccgcg ttgctcgtcc gatcgacgaa gcgccgtggc tgcaccactg cctgatggct tacgcttctg acatggcgct gctgggtact ggtaaccgtc cgcacggtat ctcctggatg accggtg Aac tgatgtccgc atctctggat cacgcgatgt ggttccacgc accgactaaa ttcgacgagt ggcacctgta ctctatggac tctccgtacg caggcggtgc acgttctttc aaccgcggtt ctatctacga

Pro Trp Cys Leu 240 Asp Leu His Gin 60 120 180 240 300 360 420 480 540 600 660 720 780 168 840 ctccaccggt cgtctggttg cttctgttgc tcaggaaggt ctgatgcgtc gcgttgaacg 900 taagccgcgt aaaagctaag gatccgagct c 931 <210> 119 <211>

<212> PRT <213> Hyphomonas neptunium Acyl-CoA thioesterase (Q0BZ30) <400>

Met Ala Lys Asp Pro Val Gly Asp Leu Leu Thr Leu Leu Asp Leu Glu 1 5 10 15

Arg Leu Glu Leu Asp Leu Phe Arg Gly Gin Ser Pro Asp Glu Glu Glu 20 25 30

Ser Gin Arg Val Phe Gly Gly Gin Val lie Ala Gin Ser Leu Val Ala 35 40 45

Ala Tyr Arg Thr Val Pro Asp Asp Arg Leu Cys His Ser Leu His Cys 50 55 60

Tyr Phe work Arg Pro Gly Asp Pro Ser Val Pro lie lie Tyr Gin Val 65 70 75 80

Asp His Ser Arg Asp Gly Gly Ser Phe Thr Thr Arg Arg Val Val Ala 85 90 95 lie Gin His Gly Lys Gin lie Phe Asn Leu Ala Ala Ser Phe His Val 100 105 110

Val Glu Asp Ser Trp His His Gin His Glu Met Pro Glu Val Asp Pro 115 120 125

Pro Glu Ser Val Gly Asp Arg lie Glu Trp Arg Arg Lys Phe Ala Glu 130 135 140

Gin Val Pro Glu Arg His Arg Gly His Phe Leu Arg Asp Arg Pro Val 145 150 155 160

Glu Met Arg Glu lie Asp Pro Leu Asp Pro Leu Lys Pro Ala Lys Ala 165 170 175

Ser Asp Gin Gin Asn Leu Trp Phe Arg Val Ala Arg Pro lie Asp Glu 180 185 190

Ala Pro Trp Leu His His Cys Leu Met Ala Tyr Ala Ser Asp Met Ala 195 200 205

Leu Leu Gly Thr Gly Asn Arg Pro His Gly lie Ser Trp Met Thr Gly 210 215 220

Glu Leu Met Ser Ala Ser Leu Asp His Ala Met Trp Phe His Ala Pro 225 230 235 240

Thr Lys Phe Asp Glu Trp His Leu Tyr Ser Met Asp Ser Pro Tyr Ala 245 250 255 169

Thr Gly Val Glu

Gly Gly Ala Arg Ser Phe Asn Arg Gly Ser lie Tyr Asp Ser 260 265 270

Arg Leu Val Ala Ser Val Ala Gin Glu Gly Leu Met Arg Arg 275 280 285

Arg Lys Pro Arg Lys Ser 290 <210> 120 <211> 1421 <212> DNA <213> Artificial <220>

<223> Acinetobacter sp. (strain ADP1), Acyl-CoA-transferase subunit A (EC=2.8.3.6) (Q937T0) <400> 120 ggtacctttt ttgggctaac aggaggaatt aaccatgatc aacaagatca tcaacgatat 60 cgaaccgatt ctgaaatcta tcccagacgg ttctaccatc atgacttctg gcttcggtac 120 taccggtcag ccggaagcgc tgctggaagc gctgatcgac ttcgcaccga aagagctgac 180 tatcatcaac aacaacgctt cttccggtcc gaacggtctg actcagctgt tcactgctgg 240 tctggttaag aaactgatct gctcctaccc gaaatctatc tcttccaccg ttttcccgga 300 cctgtaccgc gctggtaaga tcgaactgga actggttccg cagggtaacc tggcttgccg 360 tatccaggca gctggcgcgg gtctgggtgc agtattcact ccgactggtt acggtactaa 420 gatcgctgaa ggtaaagaaa ctcgtatcat caacggtaaa aactacgttc tggaataccc 480 gctggaagct gactacgcat tcatctacgc tgacaaagct gaccgctggg gtaacctgac 540 ttaccgtaaa gcggcacgta acttcggtcc Gatcatggcg aaagcagcta aaaccactat 600 tgctcaggtt aaccagactg ttgaactggg cgatctggac ccggaatgca tcatcactcc 660 gggtatcttc gtacagcacg ttgttcgtct gggtgacatc aaataatttt ttgggctaac 720 aggaggaatt aaccatgact attcagaagc gttctcgcga aga tatcgct atcatgatcg 780 ctaaagacat cccggacggt tcttacgtta acctgggtat cggtctgccg actcacgttg 840 ctaaatatct gccgaaagac aaagaaatct tcctgcactc tgaaaacggt gttctggcat 900 tcggtccgcc gccagcagaa ggtgaagaag atcaggacct ggttaacgct ggtaaagagc 960 tggtaactct gctgtctggt ggttgcttca tgcaccacgg tgactccttc gacatcatgc 1020 gtggtggtca cctggatatc tgcgttatcg gtgcattcca ggttgcgctg aacggtgacc 1080 tggctaactg gcacaccggt aaagacgacg acgttccggc agtaggtggt gcaatggacc 1140 tggctgttgg tgcgaagcgt atcttcgttt acatggaaca caccaccaag aaaggtgaac 1200 cgaaaatcgt taagcacctg acttacccga tcaccggtga acagtgcgta gaccgtatct 1260 acactgacct gtgcaccatc gaactgaaag acggtcaggc ttacgttatc gaaatggttg 1320 acggtctgga tttcgacact ctgcaggcgc tgactgaatg cccgctgatc gaccactgca 1380 cctactcttc tctgattcag ctcagataag gatccgagct c 1421

<210> 121 <211> 223 <212> PRT 170 <213> Acinetobacter sp. (strain ADP1), Acyl-CoA-transferase subunit A (EC=2.8.3.6) (Q937T0) <400>

Met lie Asn Lys lie lie Asn Asp lie Glu Pro lie Leu Lys Ser lie 15 10 15

Pro Asp Gly Ser Thr lie Met Thr Ser Gly Phe Gly Thr Thr Gly Gin 20 25 30

Pro Glu Ala Leu Leu Glu Ala Leu lie Asp Phe Ala Pro Lys Glu Leu 35 40 45

Thr lie lie Asn Asn Asn Ala Ser Ser Gly Pro Asn Gly Leu Thr Gin 50 55 60

Leu Phe Thr Ala Gly Leu Val Lys Lys Leu lie Oys Ser Tyr Pro Lys 65 70 75 80

Ser lie Ser Ser Thr Val Phe Pro Asp Leu Tyr Arg Ala Gly Lys lie 85 90 95

Glu Leu Glu Leu Val Pro Gin Gly Asn Leu Ala Cys Arg lie Gin Ala 100 105 110

Ala Gly Ala Gly Leu Gly Ala Val Phe Thr Pro Thr Gly Tyr Gly Thr 115 120 125

Lys lie Ala Glu Gly Lys Glu Thr Arg lie lie Asn Gly Lys Asn Tyr 130 135 140

Val Leu Glu Tyr Pro Leu Glu Ala Asp Tyr Ala Phe lie Tyr Ala Asp 145 150 155 160

Lys Ala Asp Arg Trp Gly Asn Leu Thr Tyr Arg Lys Ala Ala Arg Asn 165 170 175

Phe Gly Pro lie Met Ala Lys Ala Ala Lys Thr Thr lie Ala Gin Val 180 185 190

Asn Gin Thr Val Glu Leu Gly Asp Leu Asp Pro Glu Cys lie lie Thr 195 200 205

Pro Gly lie Phe Val Gin His Val Val Arg Leu Gly Asp lie Lys 210 215 220 <210> 122 <211> 224

<212> PRT <213> Acinetobacter sp. (strainADPl) Acyl-CoA-transf erase subxanit B (EC 2.8.3.6) (Q937S9) <400>

Met Thr lie Gin Lys Arg Ser Arg Glu Asp lie Ala He Met lie Ala 1 5 10 15

Lys Asp lie Pro Asp Gly Ser Tyr Val Asn Leu Gly lie Gly Leu Pro 20 25 30 171

Thr His Val Ala Lys Tyr Leu Pro Lys Asp Lys Glu lie Phe 35 40 45

Leu His

Ser Glu Asn Gly Val Leu Ala Phe Gly Pro Pro Pro Ala Glu 50 55 60

Gly Glu

Glu Asp Gin Asp Leu Val Asn Ala Gly Lys Glu Leu Val Thr 65 70 75

Leu Leu 80

Ser Gly Gly Cys Phe Met His His Gly Asp Ser Phe Asp lie 85 90

Met Arg 95

Gly Gly His Leu Asp lie Cys Val lie Gly Ala Phe Gin Val 100 105 110

Ala Leu

Asn Gly Asp Leu Ala Asn Trp His Thr Gly Lys Asp Asp Asp 115 120 125

Val Pro

Ala Val Gly Gly Ala Met Asp Leu Ala Val Gly Ala Lys Arg 130 135 140

Val Tyr Met Glu His Thr Thr Lys Lys Gly Glu Pro Lys lie 145 150 155

His Leu Thr Tyr Pro lie Thr Gly Glu Gin Cys Val Asp Arg 165 170

Thr Asp Leu Cys Thr lie Glu Leu Lys Asp Gly Gin Ala Tyr 180 185 190

Glu Met Val Asp Gly Leu Asp Phe Asp Thr Leu Gin Ala Leu 195 200 205

Cys Pro Leu lie Asp His Cys Thr Tyr Ser Ser Leu lie Gin 210 215 220 <210> 123 <211> 437 <212> PRT <213> Clostridium kluyveri (strain ATCC 8527) CoA-transferase activity (A5N390) <400> 123 Met Val Phe Lys Asn Trp Gin Asp Leu Tyr Lys Ser Lys lie 1 5 10

Ala Asp Glu Ala Val Ser Lys Val Ser Cys Gly Asp Ser lie 20 25 30

Gly Asn Ala Cys Gly Ala Ser Leu Thr Leu Leu Asp Ala Leu 35 40 45

Asn Lys Glu Lys Tyr Lys Ser Val Lys lie His Asn Leu lie 50 55 60 lie Phe Val Lys 160 lie Tyr 175 Val lie Thr Glu Leu Arg acetate Val Ser 15 lie Leu Ala Ala Leu Asn

Tyr Lys Asn Asp lie Tyr Thr Asp Pro Glu Ser Glu Lys Tyr 65 70 75

He His 80 172

Gly Asn Thr Phe Phe Val Ser Gly Gly Thr Lys Glu Ala Val Asn Cys 85 90 95 Asn Arg Thr Asp Tyr 100 Thr Pro Cys Phe Phe Tyr Glu lie Pro Lys Leu 105 110 Leu Lys Gin Lys Tyr 115 lie Asn Ala Asp Val Ala Phe lie Gin Val Ser 120 - 125 Lys Pro Asp Ser His 130 Gly Tyr Cys Ser Phe Gly Val Ser Thr Asp Tyr 135 140 Ser Gin Ala Met Val 145 Gin Ser Ala Lys Leu lie lie Ala Glu Val Asn 150 155 160 Asp Gin Met Pro Arg 165 Val Leu Gly Asp Asn Phe lie His lie Ser Asp 170 175 Met Asp Tyr lie Val 180 Glu Ser Ser Arg Pro lie Leu Glu Leu Thr Pro 185 190 Pro Lys lie Gly Glu 195 Val Glu Lys Thr lie Gly Lys Tyr Cys Ala Ser 200 205

Leu Val Glu Asp Gly Ser Thr Leu Gin Leu Gly lie Gly Ala lie Pro 210 215 220 Asp Ala Val Leu Leu 225 Phe Leu Lys Asp Lys Lys Asp Leu Gly lie His 230 235 240 Ser Glu Met lie Ser 245 Asp Gly Val Val Glu Leu Val Glu Ala Gly Val 250 255 lie Thr Asn Lys Lys 260 Lys Ser Leu His Pro Gly Lys lie lie lie Thr 265 270 Phe Leu Met Gly Thr 275 Lys Lys Leu Tyr Asp Phe lie Asn Asp Asn Pro 280 285 Met Val Glu Gly Tyr 290 Pro Val Asp Tyr Val Asn Asp Pro Lys Val lie 295 300 Met Gin Asn Ser Lys 305 Met Val Cys lie Asn Ser Cys Val Glu Val Asp 310 315 320 Phe Thr Gly Gin Val 325 Cys Ala Glu Ser Val Gly Phe Lys Gin Lie Ser 330 335

Gly Val Gly Gly Gin Val Asp Tyr Met Arg Gly Ala Ser Met Ala Asp 340 345 350 Gly Gly Lys Ser lie 355 Leu Ala lie Pro Ser Thr Ala Ala Gly Gly Lys 360 365 lie Ser Arg lie Val 370 Pro lie Leu Thr Glu Gly Ala Gly Val Thr Thr 375 380 173

Ser Arg Tyr Asp Val Gin Tyr Val Val Thr Glu Tyr Gly lie 385 390 395

Ala Leu 400

Leu Lys Gly Lys Ser lie Arg Glu Arg Ala Lys Glu Leu lie 405 410

Lys lie 415

Ala His Pro Lys Phe Arg Glu Glu Leu Thr Ala Gin Phe Glu 420 425 430

Lys Arg

Phe Ser Cys Lys Leu 435 <210> 124 <211> 538 <212> PRT <213>

Clostridium kluyveri (strain

ATCC

Succinyl-CoA: coenzyme A transferase (P38946) <400> 124 Met Ser Lys Gly lie Lys Asn Ser Gin Leu Lys Lys Lys Asn 15 10

Ala Ser Asn Val Ala Glu Lys lie Glu Glu Lys Val Glu Lys 20 25 30

Lys Val Val Glu Lys Ala Ala Glu Val Thr Glu Lys Arg lie 35 40 45

Leu Lys Leu Gin Glu Lys Val Val Thr Ala Asp Val Ala Ala 50 55 60 8527) Val Lys 15 Thr Asp Arg Asn Asp Met lie Glu Asn Gly Met lie Val Ala lie Ser Gly Phe Thr Pro 65 70 75

Ser Gly 80

Tyr Pro Lys Glu Val Pro Lys Ala Leu Thr Lys Lys Val Asn 85 90

Ala Leu 95

Glu Glu Glu Phe Lys Val Thr Leu Tyr Thr Gly Ser Ser Thr 100 105

Gly Ala 110

Asp lie Asp Gly Glu Trp Ala Lys Ala Gly lie lie Glu Arg 115 120 125

Arg lie

Pro Tyr Gin Thr Asn Ser Asp Met Arg Lys Lys lie Asn Asp 130 135 140

Gly Ser lie Lys Tyr Ala Asp Met His Leu Ser His Met Ala Gin Tyr 145 150 155 lie Asn 160

Tyr Ser Val lie Pro Lys Val Asp lie Ala lie lie Glu Ala 165 170

Val Ala 175 lie Thr Glu Glu Gly Asp lie lie Pro Ser Thr Gly lie Gly 180 185 190

Asn Thr

Ala Thr Phe Val Glu Asn Ala Asp Lys Val lie Val Glu lie 195 200 205

Asn Glu 174

Ala Gin 210 Pro Leu Glu Leu Glu Gly Met Ala Asp lie Tyr Thr Leu Lys 215 220 Asn Pro 225 Pro Arg Arg Glu Pro lie Pro lie Val Asn Ala Gly Asn Arg 230 235 240 He Gly Thr Thr Tyr Val Thr Cys Gly Ser Glu Lys lie Cys Ala lie 245 250 255 Val Met Thr Asn Thr Gin Asp Lys Thr Arg Pro Leu Thr Glu Val Ser 260 265 270 Pro Val Ser Gin Ala lie Ser Asp Asn Leu lie Gly Phe Leu Asn Lys 275 280 285 Glu Val 290 Glu Glu Gly Lys Leu Pro Lys Asn Leu Leu Pro lie Gin Ser 295 300 Gly Val 305 Gly Ser Val Ala Asn Ala Val Leu Ala Gly Leu Cys Glu Ser 310 315 320 Asn Phe Lys Asn Leu Ser Cys Tyr Thr Glu Val lie Gin Asp Ser Met 325 330 335 Leu Lys Leu lie Lys Cys Gly Lys Ala Asp Val Val Ser Gly Thr Ser 340 345 350 He Ser Pro Ser Pro Glu Met Leu Pro Glu Phe lie Lys Asp lie Asn 355 360 365 Phe Phe 370 Arg Glu Lys He Val Leu Arg Pro Gin Glu lie Ser Asn Asn 375 380 Pro Glu 385 lie Ala Arg Arg lie Gly Val lie Ser lie Asn Thr Ala Leu 390 395 400 Glu Val Asp lie Tyr Gly Asn Val Asn Ser Thr His Val Met Gly Ser 405 410 415 Lys Met Met Asn Gly lie Gly Gly Ser Gly Asp Phe Ala Arg Asn Ala 420 425 430

Tyr Leu Thr lie Phe Thr Thr Glu Ser lie Ala Lys Lys Gly Asp lie

Ser Ser 450 435 440 445 lie Val Pro Met Val Ser His Val Asp His Thr Glu His Asp 455 460 Val Met 465 Val lie Val Thr Glu Gin Gly Val Ala Asp Leu Arg Gly Leu 470 475 480

Ser Pro Arg Glu Lys Ala Val Ala lie lie Glu Asn Cys Val His Pro 485 490 495

Asp Tyr Lys Asp Met Leu Met Glu Tyr Phe Glu Glu Ala Cys Lys Ser 500 505 510 175

Ser Gly Gly Asn Thr Pro His Asn Leu Glu Lys Ala Leu Ser Trp His 515 520 525

Thr Lys Phe lie Lys Thr Gly Ser Met Lys 530 535 <210> 125 <211> 232

<212> PRT <213> Pseudomonas fluorescens (strainPf-5 / ATCCBAA-477) 3-oxoacid CoA-transferase subunit A family enzyme (Q4KEA3) <400> 125

Met Ala Gly Phe Asp Lys Arg Val Ala Ser Tyr Glu Glu Ala Leu Glu 15 10 15

Gly Leu Gin Asp Gly Met Thr Val lie Ala Gly Gly Phe Gly Leu Cys 20 25 30

Gly lie Pro Glu Asn Leu lie Ala Glu lie Lys Arg Arg Gly Thr Arg 35 40 45

Asp Leu Thr Val Val Ser Asn Asn Cys Gly Val Asp Gly Phe Gly Leu 50 55 60

Gly Val Leu Leu Glu Asp Arg Gin lie Ser Lys Val lie Ala Ser Tyr 65 70 75 80

Val Gly Glu Asn Ala Leu Phe Glu Lys Gin Leu Leu Ser Gly Glu lie 85 90 95

Glu Val Val Leu Thr Pro Gin Gly Thr Leu Ala Glu Lys Met Arg Ala 100 105 110

Gly Gly Ala Gly lie Pro Ala Phe Phe Thr Ala Thr Gly Val Gly Thr 115 120 125

Pro Val Ala Asp Gly Lys Glu Thr Arg Glu Phe Lys Gly Arg Thr Tyr 130 135 140

Leu Met Glu Glu Ser lie Thr Gly Asp Phe Ala lie Val Lys Gly Trp 145 150 155 160

Lys Ala Asp His Phe Gly Asn Val Val Tyr Arg His Thr Ala Gin Asn 165 170 175

Phe Asn Pro Leu Ala Ala Thr Ala Gly Lys lie Thr Val Val Glu Val 180 185 190

Glu Glu lie Val Glu Pro Gly Glu Leu Asp Pro Thr Gin lie His Thr 195 200 205

Pro Gly lie Tyr Val Asp Arg Val lie Cys Gly Thr Phe Glu Lys Arg 210 215 220 lie Glu Gin Arg Thr Val Arg Lys 176 225 230 <210> 126 <211>

<212> PRT <213> Pseudomonas fluorescens (strainPf-5/ATCCBAA-477) 3-oxoacid CoA-transferase subunit B family enzyme (Q4KEA4) <400>

Met Ala Leu Thr Arg Glu Gin Met Ala Gin Arg Val Ala Arg Glu Leu 15 10 15

Gin Asp Gly Phe Tyr Val Asn Leu Gly lie Gly lie Pro Thr Leu Val 20 25 30

Ala Asn Tyr lie Pro Asp Gly Met Glu Val Met Leu Gin Ser Glu Asn 35 40 45

Gly Leu Leu Gly Met Gly Ala Phe Pro Thr Glu Asp Glu Val Asp Ala 50 55 60

Asp Met lie Asn Ala Gly Lys Gin Thr Val Thr Ala Arg lie Gly Ala 65 70 75 80

Ser lie Phe Ser Ser Ala Glu Ser Phe Ala Met lie Arg Gly Gly His 85 90 95 lie Asp Leu Thr Val Leu Gly Ala Phe Glu Val Asp Val Gin Gly Asn 100 105 110 lie Ala Ser Trp Met lie Pro Gly Lys Leu Val Lys Gly Met Gly Gly 115 120 125

Ala Met Asp Leu Val Ala Gly Ala Glu Asn lie lie Val Thr Met Thr 130 135 140

His Ala Ser Lys Asp Gly Glu Ser Lys Leu Leu Ser Arg Cys Ser Leu 145 150 155 160

Pro Leu Thr Gly Ala Gin Cys lie Lys Arg Val Leu Thr Asp Leu Ala 165 170 175

Tyr Leu Glu lie Glu Asn Gly Ala Phe lie Leu Lys Glu Arg Ala Pro 180 185 190

Gly Val Ser Val Glu Glu lie Val Ser Lys Thr Ala Gly Lys Leu lie 195 200 205

Val Pro Asp His Val Pro Glu Met Gin Phe Ala 210 215 <210> 127 <211> 477

<212> PRT <213> Acinetobacter sp. NCIMB9871 6-oxohexanoate dehydrogenase (Q9R2F4) <400> 127

Met Asn Tyr Pro Asn lie Pro Leu Tyr lie Asn Gly Glu Phe Leu Asp 177 201033370 1 5 10 15 His Thr Asn Arg 20 Asp Val Lys Glu Val 25 Phe Asn Pro Val Asn 30 His Glu

Cys lie Gly Leu Met Ala Cys Ala Ser Gin Ala Asp Leu Asp Tyr Ala 35 40 45

Leu Glu Ser Ser Gin Gin Ala Phe Leu Arg Trp Lys Lys Thr Ser Pro 50 55 60 lie Thr Arg Ser Glu lie Leu Arg Thr Phe Ala Lys Leu Ala Arg Glu 65 70 75 80

Lys Ala Ala Glu lie Gly Arg Asn lie Thr Leu Asp Gin Gly Lys Pro 85 90 95

Leu Lys Glu Ala lie Ala Glu Val Thr Val Cys Ala Glu His Ala Glu 100 105 110

Trp His Ala Glu Glu Cys Arg Arg lie Tyr Gly Arg Val lie Pro Pro 115 120 125

Arg Asn Pro Asn Val Gin Gin Leu Val Val Arg Glu Pro Leu Gly Val 130 135 140

Cys Leu Ala Phe Ser Pro Trp Asn Phe Pro Phe Asn Gin Ala lie Arg 145 150 155 160

Lys lie Ser Ala Ala lie Ala Ala Gly Cys Thr lie lie Val Lys Gly 165 170 175

Ser Gly Asp Thr Pro Ser Ala Val Tyr Ala lie Ala Gin Leu Phe His 180 185 190

Glu Ala Gly Leu Pro Asn Gly Val Leu Asn Val lie Trp Gly Asp Ser 195 200 205

Asn Phe lie Ser Asp Tyr Met lie Lys Ser Pro lie lie Gin Lys lie 210 215 220

Ser Phe Thr Gly Ser Thr Pro Val Gly Lys Lys Leu Ala Ser Gin Ala 225 230 235 240

Ser Leu Tyr Met Lys Pro Cys Thr Met Glu Leu Gly Gly His Ala Pro 245 250 255

Val lie Val Cys Asp Asp Ala Asp lie Asp Ala Ala Val Glu His Leu 260 265 270

Val Gly Tyr Lys Phe Arg Asn Ala Gly Gin Val Cys Val Ser Pro Thr 275 280 285

Arg Phe Tyr Val Gin Glu Gly lie Tyr Lys Glu Phe Ser Glu Lys Val 290 295 300 178 201033370

Val Leu Arg Ala 305

Lys Gin lie Lys Val Gly 310

Cys Gly Leu Asp Ala Ser 315 320

Ser Asp Met Gly

Pro Leu Ala Gin Ala Arg 325 330

Arg Met His Ala Met Gin 335

Gin lie Val Glu 340

Asp Ala Val His Lys Gly 345

Ser Lys Leu Leu Leu Gly 350

Gly Asn Lys lie 355

Ser Asp Lys Gly Asn Phe 360

Phe Glu Pro Thr Val Leu 365

Gly Asp Leu Cys 370

Asn Asp Thr Gin Phe Met 375

Asn Asp Glu Pro Phe Gly 380

Pro lie lie Gly 385

Leu lie Pro Phe Asp Thr 390 lie Asp His Val Leu Glu 395 400

Glu Ala Asn Arg

Leu Pro Phe Gly Leu Ala 405 410

Ser Tyr Ala Phe Thr Thr 415

Ser Ser Lys Asn 420

Ala His Gin lie Ser Tyr 425

Gly Leu Glu Ala Gly Met 430

Val Ser lie Asn 435

His Met Gly Leu Ala Leu 440

Ala Glu Thr Pro Phe Gly 445

Gly lie Lys Asp 450

Ser Gly Phe Gly Ser Glu 455

Gly Gly lie Glu Thr Phe 460

Asp Gly Tyr Leu 465

Arg Thr Lys Phe lie Thr 470

Gin Leu Asn 475 <210> 128 <211> 473 <212> PRT <213> Brucella <400> 128 Met Arg lie Gly 1 melitensis 6-oxohexanoate dehydrogenase (Q8YDF1)

Lys Met Glu Met Gin Thr Arg Tyr Pro Asp Val Lys 5 10 15

Leu Phe lie Asp 20

Gly Thr Trp Arg Asp Gly 25

Ser Arg Gly Glu Thr lie 30

Glu lie Phe Asn 35

Pro Ala Thr Asp Glu Val 40 lie Gly His lie Ala Arg 45

Ala Thr Thr Ala 50

Asp Leu Asp Asp Ala Leu 55

Ala Ala Val Asp Arg Gly 60

Phe Glu Ala Trp 65

Ser Lys Val Ser Ala Phe 70

Asp Arg Tyr Lys lie Met 75 80

Arg Arg Ala Ala

Asp lie Phe Arg Ser Arg 85 90

Gly Glu Glu Val Ala Arg 95 179

Leu Leu Thr Met Glu Gin Gly Lys Pro Leu Ala Glu Ala Arg lie Glu 100 105 110 Ala Ala Ala Ala Cys Asp Leu lie Asp Trp Phe Ala Glu Glu Ala Arg 115 120 125 Arg Ser 130 Tyr Gly Arg lie Val Pro Pro Arg Gin 137 Ala Gly Cys Ser Lie Leu Leu Lys Ala Ala Glu Asp Thr Pro Ala Ala 180 185 190 Pro Ala Glu Leu Val Arg Ala Phe Ala Glu Ala Gly Leu Pro Asp Gly 195 200 205 Ala He 210 Asn Leu Val Tyr Gly Asp Pro Ala Glu lie Ser Ala Tyr Leu 215 220 lie Pro 225 His Pro Val lie Arg Lys Val Ser Phe Thr Gly Ser Thr Gin 230 235 240 Val Gly Lys Gin Leu Ala Ala Leu Ala Gly Leu His Met Lys Arg Val 245 250 255 Thr Met Glu Leu Gly Gly His Ala Pro Val lie lie Ala Ala Asp Ala 260 265 270 Asp Val Glu Gin Ala lie Lys Val Val Ser Gly Ser Lys Phe Arg Asn 275 280 285

Ala Gly Gin Val Cys lie Ser Pro Thr Arg Phe Leu lie Glu Asn Ser 290 295 300 Val Tyr 305 Asp Gin Val Val Glu Gly Met Ala Ala Tyr Ala Thr Ser Leu 310 315 320 Lys Val Gly Asp Gly Leu Glu Ala Gly Thr Thr Met Gly Pro Leu Val 325 330 335 Asn Ala Lys Arg Val Asn Ala Met Glu Arg Leu Val Gin Asp Ala Arg 340 345 350 Glu His Lys Ala Arg Val Val Thr Gly Gly Glu Arg lie Gly Asn Arg 355 360 365

Gly Asn Phe Phe Glu Pro Thr lie Leu Ala Asp Val Pro Arg Asp Ala 370 375 380 Ala lie 385 Met Asn Glu Glu Pro Phe Gly Pro Val Ala Leu Leu Asn Arg 390 395 400 180

Phe Asp Ala Leu Asp Glu Ala Leu Ser Glu Ala Asn Arg Leu Asn 405 410 415

Gly Leu Ala Ala Tyr Ala Phe Thr Gly Ser Ser Ala Lys Ala Ala 420 425 430 lie Ser Ser Thr Val Arg Ser Gly Met lie Thr lie Asn Gin Leu 435 440 445

Ser Gly Pro Ala Gly Ser Ala Leu Arg Arg Asp Gin Arg Phe Arg 450 455 460

Tyr Arg Arg Leu

Trp Asn Gly Arg Arg Cys Arg Arg Ala 465 470 <210> 129 <211> 1371 <212> DNA <213> Artificial <220><223> Pseudomonas aeruginosa (gi 9946143) <400> atgaacagcc aaatcaccaa cgccaagacc cgtgagtggc aggcgttgag ccgcgaccac catctgccgc cgttcaccga ctacaagcag ttgaacgaga agggcgcgcg gatcatcacc aaggccgaag gcgtctatat ctgggacagc gagggcaaca agatcctcga tgcgatggcc ggcctctggt gcgtcaacgt cggctacggc cgcgaggagc tggtccaggc cgccacccgg cagatgcgcg agttgccgtt ctacaacctg ttcttccaga ccgcccaccc gccggtggtc gagctggcca aggcgatcgc cgacgtcgct ccggaaggca tgaaccacgt gttcttcacc ggctccggct ccgaggccaa cgacaccgtg ctgcgtatgg tccgccacta ttgggcgacc aagggccagc cgcagaagaa agtggtgatc ggccgctgga acggctacca cggctccacc gtcgccggcg tcagcctggg cggcatgaag gcgttgcatg agcagggtga tttccccatc ccgggcatcg tccacatcgc ccagccctac tggtacggcg agggcggcga catgtcgccg gacgagttcg gcgtctgggc cgccgagcag ttggagaaga agattctcga agtgggcgag gaaaacgtcg ccgccttcat cgccgagccg atccagggcg ccggcggcgt gatcgtcccg ccggacacct actgg ccgaa gatccgcgag atcctcgcca agtacgacat cctgttcatc gccgacgaag tgatctgcgg cttcggccgt accggcgagt ggttcggcag ccagtactac ggcaacgccc cggacctgat gccgatcgcc aagggcctca cctccggcta catccccatg ggcggggtgg tggtgcgcga cgagatcgtc gaagtgctca accagggcgg cgagttctac cacggcttca cctattccgg tcacccggtg gcggccgccg tggccctgga gaacatccgc atcctgcgcg aagagaagat catcgagaag gtgaaggcgg aaacggcacc gtatttgcag aaacgctggc aggagctggc cgaccacccg ttggtgggcg aagcgcgcgg ggtcggcatg gtcgccgccc tggagctggt caagaacaag aagacccgcg agcgtttcac cgacaagggc gtcgggatgc tgtgccggga acattgtttc cgcaacggtt tgatcatgcg cgcggtgggc gacactatga ttatctcgcc gccgctggtg atcgatccgt cgcagatcga tgagttgatc accctggcgc gcaagtgcct cgatcagacc gccgccgccg tcctggcttg a 60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 181 1371 < 210 > 130 < 211 > 456

<212> PRT <213> Pseudomonas aeruginosa (gi 9946143) <400> 130

Met Asn Sex Gin lie Thr Asn Ala Lys Thr Arg Glu Trp Gin Ala Leu 15 10 15

Ser Arg Asp His His Leu Pro Pro Phe Thr Asp Tyr Lys Gin Leu Asn 20 25 30

Glu Lys Gly Ala Arg lie lie Thr Lys Ala Glu Gly Val Tyr lie Trp 35 40 45

Asp Ser Glu Gly Asn Lys lie Leu Asp Ala Met Ala Gly Leu Trp Cys 50 55 60

Val Asn Val Gly Tyr Gly Arg Glu Glu Leu Val Gin Ala Ala Thr Arg 65 70 75 80

Gin Met Arg Glu Leu Pro Phe Tyr Asn Leu Phe Phe Gin Thr Ala His 85 90 95

Pro Pro Val Val Glu Leu Ala Lys Ala lie Ala Asp Val Ala Pro Glu 100 105 110

Gly Met Asn His Val Phe Phe Thr Gly Ser Gly Ser Glu Ala Asn Asp 115 120 125

Thr Val Leu Arg Met Val Arg His Tyr Trp Ala Thr Lys Gly Gin Pro 130 135 140

Gin Lys Lys Val Val lie Gly Arg Trp Asn Gly Tyr His Gly Ser Thr 145 150 155 160

Val Ala Gly Val Ser Leu Gly Gly Met Lys Ala Leu His Glu Gin Gly 165 170 175

Asp Phe Pro lie Pro Gly lie Val His lie Ala Gin Pro Tyr Trp Tyr 180 185 190

Gly Glu Gly Gly Asp Met Ser Pro Asp Glu Phe Gly Val Trp Ala Ala 195 200 205

Glu Gin Leu Glu Lys Lys lie Leu Glu Val Gly Glu Glu Asn Val Ala 210 215 220

Ala Phe lie Ala Glu Pro lie Gin Gly Ala Gly Gly Val lie Val Pro 225 230 235 240

Pro Asp Thr Tyr Trp Pro Lys lie Arg Glu lie Leu Ala Lys Tyr Asp 245 250 255 lie Leu Phe lie Ala Asp Glu Val lie Cys Gly Phe Gly Arg Thr Gly 260 265 270 182

Glu Trp Phe Gly Ser Gin Tyr Tyr Gly Asn Ala Pro Asp Leu Met Pro 275 280 285 lie Ala Lys Gly Leu Thr Ser Gly Tyr lie Pro Met Gly Gly Val Val 290 295 300

Val Arg Asp Glu lie Val Glu Val Leu Asn Gin Gly Gly Glu Phe Tyr 305 310 315 320

His Gly Phe Thr Tyr Ser Gly His Pro Val Ala Ala Ala Val Ala Leu 325 330 335

Glu Asn worker Arg lie Leu Arg Glu Glu Lys lie lie Glu Lys Val Lys 340 345 350

Ala Glu Thr Ala Pro Tyr Leu Gin Lys Arg Trp Gin Glu Leu Ala Asp 355 360 365

His Pro Leu Val Gly Glu Ala Arg Gly Val Gly Met Val Ala Ala Leu 370 375 380

Glu Leu Val Lys Asn Lys Lys Thr Arg Glu Arg phe Thr Asp Lys Gly 385 390 395 400

Val Gly Met Leu Cys Arg Glu His Cys Phe Arg Asn Gly Leu lie Met 405 410 415

Arg Ala Val Gly Asp Thr Met lie lie Ser Pro Pro Leu Val lie Asp 420 425 430

Pro Ser Gin 435 lie Asp Glu Leu lie Thr 440 Leu Ala Arg Lys Cys Leu Asp 445 Gin Thr 450 Ala Ala Ala Val Leu 455 Ala <210> 131 <211> 70 <212> DNA <213> Artificial <;220><223> primer <400> 131 ggggacaagt ttgtacasLaa aagcaggcta ggaggaatta accatgaaca gccaaatcac 60 caacgccaag 70 <210> 132 <211> 49

<212> DNA <213 > Artificial <220><223> primer <400> 132 ggggaccact ttgtacaaga aagctgggtt caagccagga cggcggcgg 49 183 <210> 133 <211> 1353 <212> DNA <213>; Artificial < 220 > < 223 > Bacillus subtilis aminotransferase gene (gil6077991) < 400 > 133 atggagatga tggggatgga aaacattcag caaaatcagg gattaaagca aaaagatgag 60 caatttgtgt ggcatgccat gaagggagcg catcaagcgg acagcctgat agcccagaag 120 gccgaagggg cctgggtaac cgacacagac ggacgccgct atttggatgc gatgtccggt 180 ttgtggtgcg tcaacattgg ttacggcaga aaggagcttg cggaggctgc ctatgagcaa 240 ctaaaggagc tgccttacta cccgttaacg caaagtcacg cacccgcaat tcaactggcg 300 gaaaagctga atgaatggct tggcggcgat tatgttattt ttttttccaa cagcggatcg 360 gaagcaaacg aaactgcttt taaaattgcc cgccagtacc atctgcaaaa cggcgaccac 420 agccgttata aattcatctc aagatatcgg gcataccacg gcaatacatt gggagcgctc 480 tcagctaccg gacaggcgca gcggaaatat aaatacgagc ctttgagcca agggttcctg 540 catgcagctc cgccagatat ataccggaat cctgatgatg cagacacgct tga aagcgca 600 aatgaaatcg accgcatcat gacatgggaa ttaagcgaaa cgattgccgg ggtcattatg 660 gagcccatca ttacaggcgg aggcatccta atgccgccgg acggatatat gaagaaggtg 720 gaggacattt gccggcgcca cggagccctt ttgatttgcg atgaagtgat ctgcgggttt 780 ggacggacag gtgagccgtt cgggtttatg cactacggtg tgaagcctga tatcattacg 840 atggcaaagg gaatcacaag cgcgtatctg ccattgtcag cgactgctgt gaaacgggac 900 attttcgaag cgtatcaggg ggaagctcct tatgaccgtt tccgccacgt gaacacgttc 960 ggcggaagcc cggctgcctg tgctttggcg ttgaaaaacc tgcaaattat ggaggacgaa 1020 cagctgattc agcgatcccg tgatcttgga gcaaagcttt taggtgagct tcaagctctg 1080 agagaacacc cggcagtcgg ggatgttaga ggaaaagggc tgctgatcgg aatcgaactc 1140 gtcaaagaca aattgactaa agagccggct gatgccgcca aagtaaacca agtggttgcg 1200 gcgtgcaaag aaaaagggct gatcatcggc aaaaacggcg atacagtcgc cggctacaac 1260 aatgtcatcc acgttgcgcc gccattttgc ctgacagaag aggacctttc ctttatcgtg 1320 aaaacggtga aagaaagctt tcaaacgata taa 1353 < 210 > 134 < 211 > 450

<212> PRT (gil6077991) <400> 134 Met 1 Glu Met Met Gly 5 Met Glu Asn lie Gin 10 Gin Asn Gin Gly Leu 15 Lys Gin Lys Asp Glu 20 Gin Phe Val Trp His 25 Ala Met Lys Gly Ala 30 His Gin Ala Asp Ser 35 Leu lie Ala Gin Lys 40 Ala Glu Gly Ala Trp 45 Val Thr Asp <213> amino acid sequence of B. subtilis aminotransferase 184

Thr Asp Gly Arg Arg Tyr Leu Asp Ala Met Ser Gly Leu Trp Cys Val 50 55 60

Asn lie Gly Tyr Gly Arg Lys Glu Leu Ala Glu Ala Ala Tyr Glu Gin 65 70 75 80

Leu Lys Glu Leu Pro Tyr Tyr Pro Leu Thr Gin Ser His Ala Pro Ala 85 90 95 lie Gin Leu Ala Glu Lys Leu Asn Glu Trp Leu Gly Gly Asp Tyr Val 100 105 110 lie Phe Phe Ser Asn Ser Gly Ser Glu Ala Asn Glu Thr Ala Phe Lys 115 120 125 lie Ala Arg Gin Tyr His Leu Gin Asn Gly Asp His Ser Arg Tyr Lys 130 135 140

Phe lie Ser Arg Tyr Arg Ala Tyr His Gly Asn Thr Leu Gly Ala Leu 145 150 155 160

Ser Ala Thr Gly Gin Ala Gin Arg Lys Tyr Lys Tyr Glu Pro Leu Ser 165 170 175

Gin Gly Phe Leu His Ala Ala Pro Pro Asp lie Tyr Arg Asn Pro Asp 180 185 190

Asp Ala Asp Thr Leu Glu Ser Ala Asn Glu lie Asp Arg lie Met Thr 195 200 205

Trp Glu Leu Ser Glu Thr lie Ala Gly Val lie Met Glu Pro lie lie 210 215 220

Thr Gly Gly Gly lie Leu Met Pro Pro Asp Gly Tyr Met Lys Lys Val 225 230 235 240

Glu Asp lie Cys Arg Arg His Gly Ala Leu Leu lie Cys Asp Glu Val 245 250 255 lie Cys Gly Phe Gly Arg Thr Gly Glu Pro Phe Gly Phe Met His Tyr 260 265 270

Gly Val Lys Pro Asp lie lie Thr Met Ala Lys Gly lie Thr Ser Ala 275 280 285

Tyr Leu Pro Leu Ser Ala Thr Ala Val Lys Arg Asp lie Phe Glu Ala 290 295 300

Tyr Gin Gly Glu Ala Pro Tyr Asp Arg Phe Arg His Val Asn Thr Phe 305 310 315 320

Gly Gly Ser Pro Ala Ala Cys Ala Leu Ala Leu Lys Asn Leu Gin lie 325 330 335

Met Glu Asp Glu Gin Leu lie Gin Arg Ser Arg Asp Leu Gly Ala Lys 340 345 350 185

Leu Leu Gly Glu Leu Gin Ala Leu Arg Glu His Pro Ala Val Gly Asp 355 360 365

Val Arg Gly Lys Gly Leu Leu lie Gly lie Glu Leu Val Lys Asp Lys 370 375 380

Leu Thr Lys Glu Pro Ala Asp Ala Ala Lys Val Asn Gin Val Val Ala 385 390 395 400

Ala Cys Lys Glu Lys Gly Leu lie lie Gly Lys Asn Gly Asp Thr Val 405 410 415

Ala Gly Tyr Asn Asn Val lie His Val Ala Pro Pro Phe Cys Leu Thr 420 425 430

Glu Glu Asp Leu Ser Phe lie Val Lys Thr Val Lys Glu Ser Phe Gin 435 440 445

Thr lie 450 <210> 135 <211> 1407 <212> DNA <213> Artificial <220><223> Pseudomonas aeruginosa aminotransferase gene (seq ID gi9951072) <400> 135 atgaacgcaa gactgcacgc cacgtccccc ctcggcgacg ccgacctggt ccgtgccgac 60 caggcccact acatgcacgg ctaccacgtg ttcgacgacc accgcgtcaa cggctcgctg 120 aacatcgccg ccggcgacgg cgcctatatc tacgacaccg ccggcaaccg ctacctcgac 180 gcggtgggcg gcatgtggtg caccaacatc ggcctggggc gcgaggaaat ggctcgcacc 240 gtggccgagc agacccgcct gctggcctat tccaatccct tctgcgacat ggccaacccg 300 cgcgccatcg aactctgccg caagctcgcc gagctggccc ccggcgacct cgaccacgtg 360 ttcctcacca ccggcggttc caccgccgtg gacaccgcga tccgcctcat gcactactac 420 cagaactgcc gcggcaagcg cgccaagaag cacgtcatca cgcggatcaa cgcctaccac 480 ggctcgacct tcctcggcat gtcgctgggc ggcaagagcg ccgaccggcc ggccgagttc 540 gacttcctcg acgagcgcat ccaccacctc gcctgtccct attactaccg cgctccggaa 600 gggctgggcg aagccgagtt cctcgatggc ctggtggacg agttcgaacg caagatcctc 660 gaactgggcg ccgaccgggt gggggcgttc atctccgagc cggtgttcgg ctccggcggc 720 gtgatcgtcc cgcccgcggg ctaccacagg cggatgtggg agctgtgcca gcgctacgac 780 gtgctgtaca tctccgacga agtggtgacc tccttcggcc gcctcggcca cttcttcgcc 840 agccaggcgg tgttcggcgt acagccggac atcatcctca ccgccaaggg cctcacctcc 900 ggctaccagc cgctgggcgc gtgcatcttc tcccggcgca tctgggaggt gatcgccgag 960 ccggacaagg gccgctgctt cagccatggt ttcacctact ccggccaccc ggtggcctgc 1020 gcggcggcgc tgaagaacat cgagatcatc gagcgcgagg gcttgctcgc ccacgccgac 1080 186 201033370 gaggtcggcc gctacttcga ggagcgcctg caaagcctcc gcgacctgcc catcgtcggc 1140 gacgtgcgcg ggatgcgctt catggcctgt gtcgagttcg tcgccgacaa ggcgagcaag 1200 gcgctgtttc cggaaagcct gaacatcggc gagtgggtcc acctgcgggc gcagaagcgc 1260 ggcctgctgg ttcgtccgat cgtccacctg aacgtgatgt cgccgccgct gatcctcacc 1320 cgcgaacagg tcgataccgt ggtccgggtg ctgcgcgaga gcatcgagga aaccgtggag 1380 gatcttgtcc gcgccggtca ccggtaa 1407 < 210 > 136 < 211 > 468

<212> PRT

Pseudomonas aeruginosa <213 > amino acid sequence of aminotransferase (seq ID gi9951072) <400>

Met Asn Ala Arg Leu His Ala Thr Ser Pro Leu Gly Asp Ala Asp Leu 15 10 15 Lu

Val Arg Ala Asp Gin Ala His Tyr Met His Gly Tyr His Val Phe Asp 20 25 30

Asp His Arg Val Asn Gly Ser Leu Asn lie Ala Ala Gly Asp Gly Ala 35 40 45

Tyr lie Tyr Asp Thr Ala Gly Asn Arg Tyr Leu Asp Ala Val Gly Gly 50 55 60

Met Trp Cys Thr Asn lie Gly Leu Gly Arg Glu Glu Met Ala Arg Thr 65 70 75 80

Val Ala Glu Gin Thr Arg Leu Leu Ala Tyr Ser Asn Pro Phe Cys Asp 85 90 95

Met Ala Asn Pro Arg Ala lie Glu Leu Cys Arg Lys Leu Ala Glu Leu 100 105 110

Ala Pro Gly Asp Leu Asp His Val Phe Leu Thr Thr Gly Gly Ser Thr 1X5 120 125

Ala Val Asp Thr Ala lie Arg Leu Met His Tyr Tyr Gin Asn Cys Arg 130 135 140

Gly Lys Arg Ala Lys Lys His Val lie Thr Arg lie Asn Ala Tyr His 145 150 155 160

Gly Ser Thr Phe Leu Gly Met Ser Leu Gly Gly Lys Ser Ala Asp Arg 165 170 175

Pro Ala Glu Phe Asp Phe Leu Asp Glu Arg lie His His Leu Ala Cys 180 185 190

Pro Tyr Tyr Tyr Arg Ala Pro Glu Gly Leu Gly Glu Ala Glu Phe Leu 195 200 205

Asp Gly Leu Val Asp Glu Phe Glu Arg Lys lie Leu Glu Leu Gly Ala 210 215 220 187 201033370

Asp Arg Val Gly Ala Phe lie Ser Glu Pro Val Phe Gly Ser Gly Gly 225 230 235 240

Val lie Val Pro Pro Ala Gly Tyr His Arg Arg Met Trp Glu Leu Cys 245 250 255

Gin Arg Tyr Asp Val Leu Tyr lie Ser Asp Glu Val Val Thr Ser Phe 260 265 270

Gly Arg Leu Gly His Phe Phe Ala Ser Gin Ala Val Phe Gly Val Gin 275 280 285

Pro Asp lie lie Leu Thr Ala Lys Gly Leu Thr Ser Gly Tyr Gin Pro 290 295 300

Leu Gly Ala Cys lie Phe Ser Arg Arg lie Trp Glu Val lie Ala Glu 305 310 315 320 ❿

Pro Asp Lys Gly Arg Cys Phe Ser His Gly Phe Thr Tyr Ser Gly His 325 330 335

Pro Val Ala Cys Ala Ala Ala Leu Lys Asn lie Glu lie lie Glu Arg 340 345 350

Glu Gly Leu Leu Ala His Ala Asp Glu Val Gly Arg Tyr Phe Glu Glu 355 360 365

Arg Leu Gin Ser Leu Arg Asp Leu Pro lie Val Gly Asp Val Arg Gly 370 375 380

Met Arg Phe Met Ala Cys Val Glu Phe Val Ala Asp Lys Ala Ser Lys 385 390 395 400

Ala Leu Phe Pro Glu Ser Leu Asn lie Gly Glu Trp Val His Leu Arg 405 410 415

Ala Gin Lys Arg Gly Leu Leu Val Arg Pro lie Val His Leu Asn Val 420 425 430

Met Ser Pro Pro Leu lie Leu Thr Arg Glu Gin Val Asp Thr Val Val 435 440 445

Arg Val Leu Arg Glu Ser lie Glu Glu Thr Val Glu Asp Leu Val Arg 450 455 460

Ala Gly His Arg 465 <210> 137 <211> 1335 <212> DNA <213 > Artificial <220><223> Pseudomonas aeruginosa aminotransferase gene (seq ID gi9951630) <400> 137 atgacaatga atgacgagcc Gcagtcgagc agcctcgaca acttctggat gcccttcacc 60 188 201033370

gccaaccgcc agttcaaggc gcggccgcgc ctgctggaaa gcgccgaagg catccactat 120 atcgcccagg gcgggcgccg catcctcgac ggcaccgccg gcctctggtg ctgcaatgcc 180 ggccacggcc ggcgcgagat cagcgaagcg gtggcccggc agatcgccac cctcgactac 240 gccccgccgt tccagatggg tcacccgctg ccgttcgaac tcgccgcgcg gctgacggaa 300 atcgccccgc cgagcctgaa caaagtattc ttcaccaact ccggctcgga atcggcggac 360 accgcgctga agatcgccct tgcctaccag cgcgccatcg gccagggcac ccgcacccgc 420 ctgatcggcc gcgaactggg ctaccacggg gtcggcttcg gcggcctgtc ggtaggcggt 480 atggtcaaca accgcaaggc cttctccgcc aacctgctgc cgggggtcga ccacctgccg 540 cacaccctgg acgtcgcccg caacgccttc accgtcggcc tgcccgagca tggcgtggaa 600 aaggccgagg agctggaacg cctggtgacc ctgcacggcg ccgagaatat cgccgcggtg 660 atcgtcgagc cgatgtccgg ctcggccggc gtggtgctgc cgcccaaggg ctaccttcag 720 cggctgcgcg agataacccg caagcatggc atcctgctga tcttcgacga agtgatcacc 780 ggtttcggcc gcgtcggcga agccttcgcc gcgcagcgct ggggcgtcgt cccggacctg 840 ctgacctgcg ccaaggggct gaccaacggc agcatcccga tgggcgccgt attcgtcgac 900 gagaagatcc atgctgcct t catgcaaggc ccgcagggcg ccatcgagtt cttccacggc 960 tatacctatt ccggccatcc ggtagcctgc gccgccgccc tggcgaccct ggacatctac 1020 cgtcgcgacg acctgttcca gcgggccgtc gaactggaag gctactggca ggacgcgctg 1080 ttcagcctgc gcgacctgcc caacgtggtc gacatccgcg ccgtaggcct ggtcggcggc 1140 gtgcaactgg cgccgcacgc ggacggcccc ggcaagcgcg gctacgacgt cttcgagcgc 1200 tgcttctggg agcacgacct gatggtccgg gtgaccggcg acatcatcgc catgtcgccg 1260 ccgctgatca tcgacaagcc ccacatcgac cagatcgtcg agcgcctggc ccaggccatc 1320 cgcgccagcg tctga 1335 <210> 138 <211> 444

<212> PRT

<213> amino acid sequence of Pseudomonas aeruginosa aminotransferase (seq ID gi9951630) <400> 138

Met Thr Met Asn Asp Glu Pro Gin Ser Ser Ser Leu Asp Asn Phe Trp 15 10 15

Met Pro Phe Thr Ala Asn Arg Gin Phe Lys Ala Arg Pro Arg Leu Leu 50 55 60 Arg Glu lie Ser Glu Ala Val Ala Arg Gin lie Ala Thr Leu Asp Tyr 65 70 75 80 20

Glu Ser Ala Glu Gly lie His Tyr 35 40

Leu Asp Gly Thr Ala Gly Leu Trp 25 30 lie Ala Gin Gly Gly Arg Arg lie 45

Cys Cys Asn Ala Gly His Gly Arg

Ala Pro Pro Phe Gin Met Gly His 85

Pro Leu Pro Phe Glu Leu Ala Ala 90 95 189

Arg Leu Thr Glu lie Ala Pro Pro Ser Leu Asn Lys Val Phe Phe Thr 100 105 110

Asn Ser Gly Ser Glu Ser Ala Asp Thr Ala Leu Lys lie Ala Leu Ala 115 120 125

Tyr Gin Arg Ala lie Gly Gin Gly Thr Arg Thr Arg Leu lie Gly Arg 130 135 140

Glu Leu Gly Tyr His Gly Val Gly Phe Gly Gly Leu Ser Val Gly Gly 145 150 155 160

Met Val Asn Asn Arg Lys Ala Phe Ser Ala Asn Leu Leu Pro Gly Val 165 170 175

Asp His Leu Pro His Thr Leu Asp Val Ala Arg Asn Ala Phe Thr Val 180 185 190

Gly Leu Pro Glu His Gly Val Glu Lys Ala Glu Glu Leu Glu Arg Leu 195 200 205

Val Thr Leu His Gly Ala Glu Asn lie Ala Ala Val lie Val Glu Pro 210 215 220

Met Ser Gly Ser Ala Gly Val Val Leu Pro Pro Lys Gly Tyr Leu Gin 225 230 235 240

Arg Leu Arg Glu lie Thr Arg Lys His Gly lie Leu Leu lie Phe Asp 245 250 255

Glu Val lie Thr Gly Phe Gly Arg Val Gly Glu Ala Phe Ala Ala Gin 260 265 270

Arg Trp Gly Val Val Pro Asp Leu Leu Thr Cys Ala Lys Gly Leu Thr 275 280 285

Asn Gly Ser lie Pro Met Gly Ala Val Phe Val Asp Glu Lys lie His 290 295 300

Ala Ala Phe Met Gin Gly Pro Gin Gly Ala lie Glu Phe Phe His Gly 305 310 315 320

Tyr Thr Tyr Ser Gly His Pro Val Ala Cys Ala Ala Ala Leu Ala Thr 325 330 335

Leu Asp He Tyr Arg Arg Asp Asp Leu Phe Gin Arg Ala Val Glu Leu 340 345 350

Glu Gly Tyr Trp Gin Asp Ala Leu Phe Ser Leu Arg Asp Leu Pro Asn 355 360 365

Val Val Asp lie Arg Ala Val Gly Leu Val Gly Gly Val Gin Leu Ala 370 375 380

Pro His Ala Asp Gly Pro Gly Lys Arg Gly Tyr Asp Val Phe Glu Arg 190 385 390 395 400

Cys Phe Trp Glu His Asp Leu Met Val Arg Val Thr Gly 405 410

Asp lie lie 415

Ala Met Ser Pro Pro Leu lie lie Asp Lys Pro His lie 420 425

Asp Gin lie 430

VAL A A A A A A A A A A A A A A A A A A A A A A A A A A A Met Asn Gin Gin Asp lie Glu Gin Val Val Lys Ala Val 15 10 utilization

Leu Leu Lys 15

Met Gin Ser Ser Asp Thr Pro Ser Ala Ala Val His Glu 20 25

Met Gly Val 30

Phe Ala Ser Leu Asp Asp Ala Val Ala Ala Ala Lys Val 35 40 45

Ala Gin Gin

Gly Leu Lys Ser Val Ala Met Arg Gin Leu Ala lie Ala 50 55 60

Ala lie Arg

Glu Ala Gly Glu Lys His Ala Arg Asp Leu Ala Glu Leu 65 70 75

Ala Val Ser 80

Glu Thr Gly Met Gly Arg Val Glu Asp Lys Phe Ala Lys 85 90

Asn Val Ala 95

Gin Ala Arg Gly Thr Pro Gly Val Glu Cys Leu Ser Pro 100 105

Gin Val Leu 110

Thr Gly Asp Asn Gly Leu Thr Leu lie Glu Asn Ala Pro 115 120 125

Trp Gly Val

Val Ala Ser Val Thr Pro Ser Thr Asn Pro Ala Ala Thr 130 135 140

Val lie Asn

Asn Ala lie Ser Leu lie Ala Ala Gly Asn Ser Val lie 145 150 155

Phe Ala Pro 160

His Pro Ala Ala Lys Lys Val Ser Gin Arg Ala lie Thr 165 170

Leu Leu Asn 175

Gin Ala lie Val Ala Ala Gly Gly Pro Glu Asn Leu Leu 180 185

Val Thr Val 190

Ala Asn Pro Asp lie Glu Thr Ala Gin Arg Leu Phe Lys 195 200 205

Phe Pro Gly lie Gly Leu Leu Val Val Thr Gly Gly Glu Ala Val Val

Glu Ala Ala 191 210 215 220

Arg Lys His Thr Asn Lys Arg Leu lie Ala Ala Gly Ala Gly Asn Pro 225 230 235 240

Pro Val Val Val Asp Glu Thr Ala Asp Leu Ala Arg Ala Ala Gin Ser 245 250 255 lie Val Lys Gly Ala Ser Phe Asp Asn Asn lie lie Cys Ala Asp Glu 260 265 270

Lys Val Leu lie Val Val Asp Ser Val Ala Asp Glu Leu Met Arg Leu 275 280 285

Met Glu Gly Gin His Ala Val Lys Leu Thr Ala Glu Gin Ala Gin Gin 290 295 300

Leu Gin Pro Val Leu Leu Lys Asn lie Asp Glu Arg Gly Lys Gly Thr 305 310 315 320

Val Ser Arg Asp Trp Val Gly Arg Asp Ala Gly Lys lie Ala Ala Ala 325 330 335 lie Gly Leu Lys Val Pro Gin Glu Thr Arg Leu Leu Phe Val Glu Thr 340 345 350

Thr Ala Glu His Pro Phe Ala Val Thr Glu Leu Met Met Pro Val Leu 355 360 365

Pro Val Val Arg Val Ala Asn Val Ala Asp Ala lie Ala Leu Ala Val 370 375 380

Lys Leu Glu Gly Gly Cys His His Thr Ala Ala Met His Ser Arg Asn 385 390 395 400 lie Glu Asn Met Asn Gin Met Ala Asn Ala lie Asp Thr Ser lie Phe 405 410 415

Val Lys Asn Gly Pro Cys lie Ala Gly Leu Gly Leu Gly Gly Glu Gly 420 425 430

Trp Thr Thr Met Thr lie Thr Thr Pro Thr Gly Glu Gly Val Thr Ser 435 440 445

Ala Arg Thr Phe Val Arg Leu Arg Arg Cys Val Leu Val Asp Ala Phe 450 455 460

Arg lie Val 465 <210> 140 <211> 532

<212> PRT <213> Clostridium tetani Alcohol dehydrogenase (Q894U3) <400> 140

Met Glu Lys Tyr Asp Lys Asp Leu Tyr Ser lie Gin Gin Ala Arg Asn 192 201033370 1 Leu Glu Glu Gly 65 Leu Asp Met Ser Pro 145 Ala Asn Ser Ser Tyr 225 Ser lie Lys Lys 10 15

Ala Arg Leu Gly Lys Val Ala Ala Asn Lys lie Ala Asn Tyr Thr 20 25 30

Glu Gin lie Asp Lys lie Leu Arg Asn Met Val Arg Val Ala Glu 35 40 45

Asn Ala Val Ser Leu Ala Gin Met Ala Val Glu Glu Thr Gly Phe 50 55 60

Lys Val Glu Asp Lys Thr Tyr Lys Asn His Leu Ala Ala Thr lie 70 75 80

Tyr Asp Ser lie Lys Asp Met Lys Thr lie Gly Val lie Ser Glu 85 90 95

Lys Val Asn Lys Met lie Glu lie Ala Glu Pro Val Gly Leu lie 100 105 110

Gly lie, Val Pro Ser Thr Asn Pro Thr Ser Thr Ala lie Phe Lys 115 120 125 lie lie Ala lie Lys Ser Arg Asn Ala lie Val Phe Ser Pro His 130 135 140

Ala Ala Ala Lys Cys Thr lie Arg Ala Val Glu Leu Met Arg Asp 150 155 160

Ala lie Glu Ala Gly Ala Pro Glu Asp lie lie Ala Ser Leu Thr 165 170 175

Leu Thr Met Glu Ala Thr Asn Glu Leu Met Lys Asn Glu Asn Val 180 185 190

Leu lie lie Ala Thr Gly Gly Pro Gly Met Val Lys Ala Ala Tyr 195 200 205

Ser Gly Lys Pro Ala lie Gly Val Gly Ala Gly Asn Ser Pro Ala 210 215 220 lie Glu Arg Thr Ala Asn Val Glu Lys Ala lie Arg Asp lie lie 230 235 240

Ser Lys Ser Phe Asp Tyr Gly Thr lie Cys Ala Ser Glu Gin Ser 245 250 255 lie Cys Glu Glu Cys Asn His Asp Ala Val Val Glu Glu Leu Lys 260 265 270

Gin Gly Gly Tyr Phe Met Thr Ala Glu Glu Thr Glu Lys Val Cys 275 280 285

Leu Leu Phe Lys Asn Gly His Thr Met Asn Ala Lys Phe Val Gly 290 295 300 193

Arg Ser Pro Gin Val lie Ala Asn Ala Ala Gly Phe Thr Val Ser Glu 305 310 315 320

Asp lie Lys Val Leu L Gly Lys Gin Asn Gly Val Gly Asn Gly Asn 325 330 335 Pro Leu Ser Phe Glu Lys Leu Thr Thr Val Leu Ala Phe Tyr Thr Val 340 345 350 Lys Asp Trp His Glu Ala Cys Glu Leu Ser lie Glu Leu Leu Gin Asn 355 360 365 Gly He 370 Gly His Thr Met Ser lie His Thr Gin Asn Asp Asp lie Val 375 380 Met Glu 385 Phe Ala Lys Lys Pro Ala Ser Arg lie Leu Val Asn Thr Gly 390 395 400 Gly Ser Gin Gly Gly Thr Gly Ala Ser Thr Gly Leu Lys Pro Ala Phe 405 410 415 Thr Leu Gly Cys Gly Thr Trp Gly Gly Ser Ser Val Ser Glu Asn Val 420 425 430 Thr Pro Glu His Leu lie Asn Lys Lys Arg Val Ala Tyr Gly Leu Lys 435 440 445 Asp Cys 450 Ala Thr Leu Val Gin Asn Asp Pro Thr Phe Asn Cys lie Lys 455 460 Thr Ala 465 Ser Asn Cys Arg Gly Val Lys Asn Gin Phe Met Asn Met Ser 470 475 480 Pro Ala Gin lie Ala Ala Ala Ala Glu Val Leu Asn Lys Cys Asn Asp 485 490 495 Tyr Ala Lys Asn Thr Gly Cys Ser Asn Glu Ser Lys Glu Lys Asp Ala 500 505 510

Asn Asn Glu Glu Leu Leu Ser Leu Val Asn Gin lie Val Ala Ala Met 515 520 525

Lys Gly Ala Asn 530 <210> 141 <211> 297

<212> PRT <213> Burkholderia thailandensis (strain E264 / ATCC 700388 ) Acetaldehyde dehydrogenase (Q2T7T0) <400>

Met Lys 1 Asn Lys Ser Ser Arg Thr Arg Val Ala lie Leu Gly Ser Gly 5 10 15 Ser lie Gly Leu Asp Leu Met Phe Lys Val Lys Ala Ser Glu His Phe 20 25 30 194

Asp Leu Lys Phe Val Val Gly Arg His Ala Asn Ser Asp Gly Leu Lys 35 40 45

Leu Ala Arg Ser Cys Asn Val Glu Thr Ser Ser Asp Gly Leu Asp Phe 50 55 60

Leu Lys Glu Asn Glu Asp Ala Tyr Asp Leu Val Phe Asp Ala Thr Ser 65 70 75 80

Ala Ala Ala His Lys Val Asn Asn Gly Phe Phe Ser Gly Ala Gly Lys 85 90 95

Phe Val work Le Asp Leu Thr Pro Ala Lys Leu Gly Arg Leu Cys Val Pro 100 105 110

Cys lie Asn Leu Asp Asp lie Gly Ala Glu Gin Asn Val Asn Leu lie 115 120 125

Thr Cys Gly Gly Gin Ala Ser Leu Pro Leu Ala Tyr Ala Leu Lys Gin 130 135 140

Ala Val Asp Glu lie Glu Tyr Leu Glu Val Val Ser Ala lie Ala Ser 145 150 1S5 160

Arg Ser Ala Gly lie Ala Thr Arg Glu Asn lie Asp Glu Tyr Met Thr 165 170 175

Thr Thr Glu Tyr Ala Leu Ala Gin Phe Ser Gly Ala Lys Lys Thr Lys 180 185 190

Ala lie Leu Asn lie Asn Pro Ala Glu Pro Gly Val Arg Met Gin Thr 195 200 205

Thr Leu Tyr Ala His Ala Arg Tyr Arg Asp Phe Asp Arg Val Arg Ala 210 215 220

Cys Val Ala Glu Met Val Glu Lys Val Arg Glu Tyr Val Pro Gly Tyr 225 230 235 240

Arg Leu Val Val Glu Pro lie Glu Ser Gin Gly Arg lie Thr lie Ser 245 250 255

Leu Thr Val Arg Gly Arg Gly Asp Tyr Leu Pro Glu Tyr Ala Gly Asn 260 265 270

Leu Asp lie lie Asn Cys Ala Ala Leu Ala Val Ala Ser His Arg His 275 280 285

Ala Thr Ala Arg Leu Gly Ala Thr Gin 290 295 >>>> V > o 1 2 3 E o 11119 o 2 2 2 2 Q 4 <<<<< d'ue PS 2 6 T p 4 1 R 1 3 p 2 2 4 sano adi tu p

Acylating aldehyde dehydrogenase 195

Met Thr Arg Lys Val Lys Ala Ala lie lie Gly Ser Gly Asn lie Gly 15 10 15

Thr Asp Leu Met lie 20 Lys lie Leu Arg His Gly Gin His lie Glu Met 25 30 Gly Ala Met Val Gly 35 lie Asp Pro Ala Ser Asp Gly Leu Ala Arg Ala 40 45

Gin Arg Met Gly Val Ala lie Thr His Glu Gly Val Glu Gly Leu Thr 50 55 60

Arg Leu Pro Val Phe Asn Glu lie Asp Val Val Phe Asp Ala Thr Ser 65 70 75 80 Ala Gly Ala His Val 85 Lys Asn Glu Ala Leu Leu Arg Glu Arg Lys Pro 90 95

Gly Leu Arg Met lie Asp Leu Thr Pro Ala Ala lie Gly Pro Tyr Cys 100 105 110 lie Pro Val Val Asn 115 Gly Asp Asp His Leu Asp Ala Thr Asn Val Asn 120 125 Met Val Thr Cys Gly 130 Gly Gin Ala Thr lie Pro Met Val Ala Ala Val 135 140 Ser Arg Val Ala Lys 145 Val His Tyr Ala Glu lie lie Ala Ser lie Ser 150 155 160 Ser Lys Ser Ala Gly 165 Pro Gly Thr Arg Ala Asn lie Asp Glu Phe Thr 170 175 Glu Thr Thr Ser Lys 180 Ala lie Glu Val Val Gly Gly Ala Ala Lys Gly 185 190 Lys Ala lie He Val 195 Leu Asn Pro Ala Glu Pro Pro Leu Met Met Arg 200 205 Asp Thr Val Tyr Thr 210 Leu Ser Asp Phe Ala Asp lie Asp Gin lie Glu 215 220 Glu Ser Val Gin Arg 225 Met Ala Asp Ala Val Gin Ala Tyr Val Pro Gly 230 235 240

Tyr Arg Leu Lys Gin Arg Val Gin Phe Asp Arg lie Glu Ala Asp Cys 245 250 255 Pro lie Arg lie Pro 260 Gly Val Gly Asp Arg Met Asn Gly Leu Lys Thr 265 270

Ser lie Phe Leu Glu Val Glu Gly Ala Ala His Tyr Leu Pro Ala Tyr 275 280 285

Ala Gly Asn Leu Asp lie Met Thr Ser Ala Ala Leu Arg Thr Ala Glu 290 295 300 196

Lys Leu Ala Glu Arg Leu Leu Ala Ser Leu Val Ala 305 310 315 <210> 143 <211> 539

<212> PRT <213> Burkholderia mallei Acetaldehyde dehydrogenase (Acetylating) (A5XRR2) <400> 143

Met His Arg Gly Ala Ala Cys Trp His Gly Phe Gly Val Thr Asn Gly 15 10 15

Val Ala His Val Ala Arg Arg lie Thr Asp Thr Asp Met Arg Gly Ala 20 25 30

Thr Met Asn His Ala Asp Met Gin His Leu Asn lie Glu Phe Pro Tyr 35 40 45

Arg Lys Gin Tyr Gly Asn Phe lie Gly Gly Glu Trp Val Ala Pro Val 50 55 60

Gly Gly Glu Tyr Phe Asp Asn Val Ser Pro Val Thr Gly Arg Pro Phe 65 70 75 80

Thr Ala lie Pro Arg Ser Arg Glu Ala Asp lie Glu Leu Ala Leu Asp 85 90 95

Ala Ala His Ala Ala Lys Ala Gly Trp Ala Ala Lys Gly Ala Ala Glu 100 105 110

Arg Ala Asn Val Leu Leu Arg lie Ala Asp Arg Met Glu Ala Asn Leu 115 120 125

Thr Arg Leu Ala Val Ala Glu Thr lie Asp Asn Gly Lys Pro Leu Arg 130 135 140

Glu Thr Thr Ala Ala Asp Val Pro Leu Ala lie Asp His Phe Arg Tyr 145 150 155 160

Phe Ala Gly Cys lie Arg Ala Gin Glu Gly Ser lie Ala Asp lie Gly 165 170 175

Gly Asp Met Val Ala Tyr His Phe His Glu Pro Leu Gly Val Val Gly 180 185 190

Gin lie lie Pro Trp Asn Phe Pro Leu Leu Met Ala Ala Trp Lys Leu 195 200 205

Ala Pro Ala Leu Ala Ala Gly Asn Cys Val Val Leu Lys Pro Ala Glu 210 215 220

Gin Thr Pro Ala Ser lie Leu Val Phe Ala Glu Leu lie Gin Asp Leu 225 230 235 240

Leu Pro Pro Gly Val Leu Asn lie Val Asn Gly Phe Gly Leu Glu Ala 245 250 255 197

Gly Lys Pro

Leu Ala Ser Ser Lys Arg lie Ala Lys lie Ala Phe Thr 260 265 270

Gly Glu Thr 275

Ser Thr Gly Arg Leu lie Met Gin Tyr Ala Ser Glu Asn 280 285

Leu lie Pro 290

Val Thr Leu Glu Leu Gly Gly Lys Ser Pro Asn lie Phe 295 300

Phe Ala Asp 305

Val Met Asp Arg Asp Asp Ser Tyr Phe Asp Lys Ala Leu 310 315 320

Glu Gly Phe

Ala Met Phe Ala Leu Asn Gin Gly Glu Val Cys Thr Cys 325 330 335

Pro Ser Arg

Ala Leu Val Glu Glu Ser lie Tyr Asp Arg Phe lie Glu 340 345 350

Arg Ala Leu 355

Lys Arg Val Glu Ala lie Lys Gin Gly His Pro Leu Asp 360 365

Ser Gin Thr 370

Met lie Gly Ala Gin Ala Ser Ala Glu Gin Leu Glu Lys 375 380 lie Leu Ser 385

Tyr lie Asp lie Gly Arg Gly Glu Gly Ala Gin Cys Leu 390 395 400

Thr Gly Gly

Glu Arg Asn Val Leu Gly Gly Glu Leu Ala Glu Gly Tyr 405 410 415

Tyr Val Lys

Pro Thr Val Phe Arg Gly His Asn Lys Met Arg lie Phe 420 425 430

Gin Glu Glu 435 lie Phe Gly Pro Val Leu Ala Val Thr Thr Phe Lys Thr 440 445

Glu Glu Glu 450

Ala Leu Glu lie Ala Asn Asp Thr Leu Tyr Gly Leu Gly 455 460

Ala Gly Val 465

Trp Thr Arg Asp Gly Asn Arg Ala Tyr Arg Phe Gly Arg 470 475 480

Gly lie Gin

Ala Gly Arg Val Trp Thr Asn Cys Tyr His Ala Tyr Pro 485 490 495

Ala His Ala

Ala Phe Gly Gly Tyr Lys Gin Ser Gly lie Gly Arg Glu 500 505 510

Thr His Lys 515

Met Met Leu Asp His Tyr Gin Gin Thr Lys Asn Leu Leu 520 525

Val Ser Tyr 530

Ser Glu Lys Pro Leu Gly Phe Phe 535 <210> 144 <211> 464 198 201033370

<212> PRT <213> Salmonella typhimurium Propanediol utilization CoA-dependent propionaldehyde dehydrogenase (Q9XDN1) <400>

Met Asn Thr Ser Glu Leu Glu Thr Leu lie Arg Thr lie Leu Ser Glu 15 10 15

Gin Leu Thr Thr Pro Ala Gin Thr Pro Val Gin Pro Gin Gly Lys Gly 20 25 30 lie Phe Gin Ser Val Ser Glu Ala lie Asp Ala Ala His Gin Ala Phe 35 40 45

Leu Arg Tyr Gin Gin Cys Pro Leu Lys Thr Arg Ser Ala lie lie Ser 50 55 60

Ala Met Arg Gin Glu Leu Thr Pro Leu Leu Ala Pro Leu Ala Glu Glu 65 70 75 80

Ser Ala Asn Glu Thr Gly Met Gly Asn Lys Glu Asp Lys Phe Leu Lys 85 90 95

Asn Lys Ala Ala Leu Asp Asn Thr Pro Gly Val Glu Asp Leu Thr Thr 100 105 110

Thr Ala Leu Thr Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro 115 120 125

Phe Gly Val lie Gly Ser Val Ala Pro Ser Thr Asn Pro Thr Glu Thr 130 135 140 lie lie Asn Asn Ser lie Ser Met Leu Ala Ala Gly Asn Ser lie Tyr 145 150 155 160

Phe Ser Pro His Pro Gly Ala Lys Lys Val Ser Leu Lys Leu lie Ser 165 170 175

Leu lie Glu Glu lie Ala Phe Arg Cys Cys Gly lie Arg Asn Leu Val 180 185 190

Val Thr Val Ala Glu Pro Thr Phe Glu Ala Thr Gin Gin Met Met Ala 195 200 205

His Pro Arg lie Ala Val Leu Ala lie Thr Gly Gly Pro Gly lie Val 210 215 220

Ala Met Gly Met Lys Ser Gly Lys Lys Val lie Gly Ala Gly Ala Gly 225 230 235 240

Asn Pro Pro Cys lie Val Asp Glu Thr Ala Asp Leu Val Lys Ala Ala 245 250 255

Glu Asp lie lie Asn Gly Ala Ser Phe Asp Tyr Asn Leu Pro Cys lie 260 265 270

Ala Glu Lys Ser Leu lie Val Val Glu Ser Val Ala Glu Arg Leu Val 199 275 280 285

Gin Gin Met Gin Thr 290 Phe Gly Ala Leu Leu Leu Ser Pro Ala Asp Thr 295 300 Asp Lys Leu Arg Ala 305 Val Cys Leu Pro Glu Gly Gin Ala Asn Lys Lys 310 315 320 Leu Val Gly Lys Ser 325 Pro Ser Ala Met Leu Glu Ala Ala Gly lie Ala 330 335 Val Pro Ala Lys Ala 340 Pro Arg Leu Leu lie Ala Leu Val Asn Ala Asp 345 350 Asp Pro Trp Val Thr 355 Ser Glu Gin Leu Met Pro Met Leu Pro Val Val 360 365 Lys Val Ser Asp Phe 370 Asp Ser Ala Leu Ala Leu Ala Leu Lys Val Glu 375 380 Glu Gly Leu His His 385 Thr Ala lie Met His Ser Gin Asn Val Ser Arg 390 395 400 Leu Asn Leu Ala Ala 405 Arg Thr Leu Gin Thr Ser lie Phe Val Lys Asn 410 415 Gly Pro Ser Tyr Ala 420 Gly lie Gly Val Gly Gly Glu Gly Phe Thr Thr 425 430 Phe Thr lie Ala Thr 435 Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Thr 440 445 Phe Ala Arg Ser Arg 450 Arg Cys Val Leu Thr Asn Gly Phe Ser lie Arg 455 460 <210> 145 <211> 315 <212> PRT <213> Rhodococcus sp. (strain RHA1) Acetaldehyde dehydrogenase (Q0SJD4) <400>

Met Ser Thr Thr Lys 1 5 Val Ala Val lie Gly Ser Gly Asn lie Gly Thr 10 15 Asp Leu Val lie Lys 20 Leu Glu Gin Val Ala Lys Asn Val Glu lie Ala 25 30 Val Leu Val Gly lie 35 Asp Pro Ala Ser Asp Gly Leu Ala Arg Ala Arg 40 45 Arg Leu Gly lie Ser 50 Thr Val Asp Thr Gly Val Gin Gly Leu lie Glu 55 60 His Pro Glu Phe Asp 65 Asp lie Glu lie lie Phe Asp Ser Thr Ser Ala 70 75 80 Lys Ala His Leu Thr Asn Ala Glu Ala Leu Arg Pro Tyr Gly Lys His 200 85 90 95

Leu lie Asp Leu Thr Pro Ala Ala lie Gly Pro Tyr Val Val Pro Ala 100 105 110

Val Asn Leu Asp Gin His Leu Gly Ala Pro Asp Val Asn Met Val Thr 115 120 125

Cys Gly Gly Gin Ala Thr lie Pro lie Val Ala Ala lie Ser Lys Val 130 135 140

Thr Asp Val His Tyr Ala Glu lie Val Ala Ser lie Ala Ser Lys Ser 145 150 155 160

Ala Gly Pro Gly Thr Arg Ala Asn lie Asp Glu Phe Thr Glu Thr Thr 165 170 175

Ala Lys Ala lie Glu Glu Val Gly Gly Ala Ala His Gly Lys Ala lie 180 185 190 lie Val Leu Asn Pro Ala Asp Pro Pro Leu lie Met Arg Asp Thr Val 195 200 205

Leu Ala Leu Val Ser Asn Pro Asp Gin Asp Ala lie Arg Gin Ser lie 210 215 220

Thr Asp Met Val Ala Thr Val Ala Ala Tyr Val Pro Gly Tyr Arg Leu 225 230 235 240

Lys Gin Asp Val Gin Phe Thr Glu Leu Gly Gly Ala Asp Thr Val Ala 245 250 255

Thr Leu Thr Gly Gly Ala Asp lie Gly Ser Pro Val Trp Lys Val Ser 260 265 270

Val Phe Leu Glu Val Glu Gly Ala Ala His Tyr Leu Pro Ala Tyr Ala 275 280 285

Gly Asn Leu Asp lie Met Thr Ser Ala Ala Leu Gin Val Ala Glu Lys 290 295 300

Leu Ala Glu Arg Thr Ala Leu Glu lie Ala Arg 305 310 315 <210> 146

<211> 468 <212> PRT <213> Clostridium beijerinckii Coenzyme A acylating aldehyde dehydrogenase (Q9X681) <400>

Met Asn Lys Asp Thr Leu lie Pro Thr Thr Lys Asp Leu Lys Leu Lys 15 10 15

Thr Asn Val Glu Asn lie Asn Leu Lys Asn Tyr Lys Asp Asn Ser Ser 20 25 30 201

Cys Phe Gly Val Phe Glu Asn Val Glu Asn Ala lie Asn Ser Ala Val 35 40 45

His Ala Gin Lys lie Leu Ser Leu His Tyr Thr Lys Glu Gin Arg Glu 50 55 60

Lys lie work le Thr Glu lie Arg Lys Ala Ala Leu Glu Asn Lys Glu Val 65 70 75 80

Leu Ala Thr Met lie Leu Glu Glu Thr His Met Gly Arg Tyr Glu Asp 85 90 95

Lys lie Leu Lys His Glu Leu Val Ala Lys Tyr Thr Pro Gly Thr Glu 100 105 110

Asp Leu Thr Thr Thr Ala Trp Ser Gly Asp Asn Gly Leu Thr Val Val 115 120 125

Glu Met Ser Pro Tyr Gly Val lie Gly Ala lie Thr Pro Ser Thr Asn 130 135 140

Pro Thr Glu Thr Val lie Cys Asn Ser lie Gly Met lie Ala Ala Gly 145 150 155 160

Asn Ala Val Val Phe Asn Gly His Pro Gly Ala Lys Lys Cys Val Ala 165 170 175

Phe Ala lie Glu Met lie Asn Lys Ala lie lie Ser Cys Gly Gly Pro 180 185 190

Glu Asn Leu Val Thr Thr lie Lys Asn Pro Thr Met Glu Ser Leu Asp 195 200 205

Ala lie lie Lys His Pro Leu lie Lys Leu Leu Cys Gly Thr Gly Gly 210 215 220

Pro Gly Met Val Lys Thr Leu Leu Asn Ser Gly Lys Lys Ala lie Gly 225 230 235 240

Ala Gly Ala Gly Asn Pro Pro Val lie Val Asp Asp Thr Ala Asp lie 245 250 255

Glu Lys Ala Gly Lys Ser lie lie Glu Gly Cys Ser Phe Asp Asn Asn 260 265 270

Leu Pro Cys lie Ala Glu Lys Glu Val Phe Val Phe Glu Asn Val Ala 275 280 285

Asp Asp Leu lie Ser Asn Met Leu Lys Asn Asn Ala Val lie lie Asn 290 295 300

Glu Asp Gin Val Ser Lys Leu lie Asp Leu Val Leu Gin Lys Asn Asn 305 310 315 320

Glu Thr Gin Glu Tyr Phe lie Asn Lys Lys Trp Val Gly Lys Asp Ala 325 330 335 202

Lys Leu Phe Ser Asp Glu lie Asp Val Glu Ser Pro Ser Asn 340 345 350 lie Lys

Cys lie Val Cys Glu Val Asn Ala Asn His Pro Phe Val Met 355 360 365

Thr Glu

Leu Met Met Pro lie Leu Pro lie Val Arg Val Lys Asp lie 370 375 380

Asp Glu

Ala Val Lys Tyr Thr Lys lie Ala Glu Gin Asn Arg Lys His 385 390 395

Ser Ala 400

Tyr lie Tyr Ser Lys Asn lie Asp Asn Leu Asn Arg Phe Glu 405 410

Arg Glu 415 lie Asp Thr Thr lie Phe Val Lys Asn Ala Lys 420 425

Ser Phe Ala 430

Gly Val

Gly Tyr Glu Ala Glu Gly Phe Thr Thr Phe Thr 435 440 lie Ala Gly 445

Ser Thr

Gly Glu Gly lie Thr Ser Ala Arg Asn Phe Thr Arg Gin Arg 450 455 460

Arg Cys

Val Leu Ala Gly 465 <210> 147 <211> 475 <212> PRT <213> Bacillus thuringiensis serovar israelensis dehydrogenase / Acetaldehyde dehydrogenase (Acetylating) <400> 147 Met Thr Asn Lys Thr Asp Glu Lys Glu Val Gin Ala Met Val 15 10

Alcohol (Q3EZP1) Asn Val 15

Val lie Lys Asp Glu Asn Asp Val Gin Glu Val Val Asn Thr 20 25 30

Leu Val

Glu Asn Gly Lys Glu Ala Leu Lys Ala Leu Glu Ser Tyr Thr 35 40 45 Gin Val Asp His lie Val His Glu Met Ala Leu Ser Gly Leu 50 55 60

Gin Glu

Asp Gin

His Met Pro Leu Ala Lys Met Ala Val Glu Glu Thr Gly Arg 65 70 75

Gly Val 80

Tyr Glu Asp Lys Cys Thr Lys Asn lie Phe Ala 85 90

Thr Glu Tyr lie Trp 95

His Ser lie Lys Lys Asp Lys Thr Val Gly lie 100 105 lie His Glu 110

Asp Pro

His Glu Glu Val lie Glu lie Ala Glu Pro Val 115 120

Gly Val Val 125

Ala Gly 203

Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Met Phe Lys Ala lie 130 135 140 lie Ala Met Lys Thr Arg Asn Pro lie lie Phe Ala Phe His Pro Ser 145 150 155 160

Ala Gin Gin Cys Ser Val Ala Ala Ala Lys lie Leu Arg Asp Ala Ala 165 170 175 lie Arg Ala Gly Ala Pro Glu Asn Cys lie Gin Trp He Glu Lys Pro 180 185 190

Ser Val Glu Ala Thr Lys Arg Leu Met Asn His Glu Gly Val Ala Leu 195 200 205

Val Leu Ala Thr Gly Gly Ala Gly Met Val Lys Ser Ala Tyr Ser Thr 210 215 220

Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Val Pro Cys Tyr Leu 225 230 235 240

Glu Lys Ser Ala His Val Lys Arg Ala Val Asn Asp Leu lie Leu Ser 245 250 255

Lys Thr Phe Asp Asn Gly Met lie Cys Ala Ser Glu Gin Ala lie lie 260 265 270

Val Asp Lys Glu lie Tyr Asn Gly Val Lys Lys Glu Met Gin Asp Asn 275 280 285

Asn Cys Tyr Phe Val Thr Glu Glu Glu Arg lie Lys Leu Glu Lys Leu 290 295 300

Val lie Asn Glu Asn Thr Cys Ala Val Asn Ser Asp lie Val Gly Lys 305 310 315 320

Ser Ala His Tyr lie Ala Ser Leu Val Ser lie Lys Val Pro Glu Asp 325 330 335

Thr Lys lie Leu Val Ala Glu lie Lys Gly Val Gly Ala Glu Tyr Pro 340 345 350

Leu Ser Arg Glu Lys Leu Ser Pro Val Leu Ala Cys lie Lys Ala Asn 355 360 365

Ser Gin Glu Glu Gly Phe Lys Tyr Cys Glu Glu Met Leu Asn Leu Gly 370 375 380

Gly Leu Gly His Ser Ala Val lie His Ser Thr Asn Lys Glu Val Gin 385 390 395 400

Lys Gin Phe Gly Leu Arg Met Lys Ala Cys Arg Leu lie Val Asn Ser 405 410 415

Pro Ser Ala Gin Gly Gly lie Gly Asp lie Tyr Asn Ala Phe lie Pro 420 425 430 204

Ser Leu Thr Leu Gly Cys Gly Ser Tyr Gly Lys Asn Ser Val Ser Gin 435 440 445

Asn Val Thr Ala Thr His lie lie Asn Val Lys Arg Leu Ala Asn Ser 450 455 460

Pro Asp Asn Met Arg Gly Phe Thr lie Thr Thr 465 470 475 <210> 148 <211> 467

<212> PRT <213 > marine gamma proteobacterium Aldehyde dehydrogenase (NAD) family protein (B7RWJ8) <400> 148

Met Ser Glu Ala Gin Ser Pro Ala Gin Glu Val Ala Glu Met Met Ser 15 10 15

Arg Ala Arg Ala Ala Gin Ala Gin lie Ala Asp Tyr Thr Gin Glu Gin 20 25 30

Val Asp Glu Leu lie Thr Ala Met Val Tyr Ala Val Ala Arg Glu Asp 35 40 45

Arg Ser Glu Glu lie Ala Arg Phe Thr Val Glu Glu Thr Gin Leu Gly 50 55 60

Asn Tyr Glu Gly Lys Tyr Leu Lys lie His Arg Lys Thr Arg Ala Thr 65 70 75 80

Leu Met Asp lie lie Asp Asp Lys Ser Val Gly Val lie Glu Glu Leu 85 90 95

Pro Glu Arg Asn lie Val Lys lie Ala Lys Pro Val Gly Val lie Gly 100 105 110

Ala Leu Ser Pro Ser Thr Asn Pro Glu Ala Thr Pro Val lie Lys Ala 115 120 125 lie Ser Ala Val Lys Gly Arg Asn Ala lie lie Val Ala Pro His Pro 130 135 140

Arg Ala Lys Leu Thr Asn Lys Lys lie Cys Asp Tyr Met Arg Glu Ala 145 150 155 160

Leu Glu Leu Cys Gly Ala Pro Ala Asp Leu Val Gin Ser lie Asp Val 165 170 175

Pro Ser Leu Asp Lys Thr Asn Glu Leu Met Ala Gin Cys Asp Arg Val 180 185 190

Leu Ala Thr Gly Gly Glu Ala Met Val Thr Ala Ala Tyr Ser Ser Gly 195 200 205

Thr Pro Ala Leu Gly Val Gly Val Gly Asn Ala Val lie Thr Val Asp 210 215 220 205

Asp Thr Ala Asp Leu Asp Glu Ala Ala Glu Lys lie Arg lie Ser 225 230 235

Thr Leu Asp Leu Ala Ala Ser Cys Ser Ser Asp Asn Ser Val Leu 245 250 255

Phe Glu Ser Val Tyr Asn Glu Leu Leu Glu Lys Leu Lys His Glu 260 265 270

Gly Phe Val lie Glu Gly Asp Asp Lys Ala Lys Leu Gin Ser Val 275 280 285

Trp Glu Asp Asp His Leu Asn Ala Lys lie Val Ala Gin His Ala 290 295 300

Thr lie Cys Gly Gin Ala Gly lie Asp Leu Pro Glu Gly Lys Ser 305 310 315

Leu lie Val Pro Glu Thr Gly Ala Gly Ala Asp His Pro Phe Ser 325 330 335

Glu Lys Leu Thr Val Thr Met Ala Leu Tyr Lys Val Lys Asp lie 340 345 350

Glu Ala lie Glu Met Thr Asn Lys lie Gin Ala Tyr Gin Gly Gin 355 360 365

His Ser Cys Gly lie Tyr Ser Tyr Asn Asp Asp Asn Val Leu Lys 370 375 380

Ala Asp Ala Thr Arg Thr Ser Arg Val Met Val Asn Gin Pro Gin 385 390 395

Ala Ser Asn Ser Gly Asn Leu Trp Asn Gly Met Arg Gin Thr Phe 405 410 415

Leu Gly Cys Gly Ser Trp Gly Gly Asn Ser lie Asn His Asn lie 420 425 430

Trp Arg Asp Leu lie Asn Glu Thr Trp Val Ser Lys Pro Leu Ala 435 440 445

Thr Lys Thr lie Pro Ser Asp Glu Glu Leu Phe Gly Ala Val Met 450 455 460

Lys 240 Val Gly He Gin Phe 320 Gly Asp Gly Leu Ala 400 Ser Thr Thr Ala

Lys Phe Ser 465 206

Claims (1)

201033370 VII. Patent application scope: 1. A method for preparing adipate or thiodicarboxylate, comprising 2,3-dehydroadipate or 2,3-dehydrogenation in the presence of a biocatalyst The thioester of adipate is converted to adipate or thiodicarboxylate. 2. The method of claim 1, wherein the biocatalyst comprises an enzyme that catalyzes the reduction of a carbon-carbon double bond of a 2,3 enoic acid moiety or a 2-olefinic moiety. 3. The method of claim 2, wherein the biocatalyst comprises an oxidoreductase (EC 1·3·1· or 1.3.99) selected from the group consisting of HC-CH groups acting on the donor body. The enzyme in the group is preferably selected from the group consisting of oxidoreductases (EC 1.3.1 and EC 1.3.99), preferably selected from the enol-CoA reductase (EC 1.3). .1.8, EC 1.3.1.38 and ugly 1.1.4.44) consist of a group consisting of dilute-[stuffed-carrier-protein] reductase EC 1.3.1.9, EC 1.3.1.10 and EC 1.3. a group consisting of 1.39; and selected from the group consisting of Dings-CoA dehydrogenase (EC 1.3.99.2), 醯_c〇A dehydrogenase (1.3.99.3), and long-chain 醯C〇A dehydrogenase Group of (EC 1.3.99.13). 4. The method of any one of the preceding claims, wherein the biocatalyst comprises an enzyme selected from the group consisting of sequence IDs 42-67, 94, 96, 98, 100, 1, 2, 1 An amino acid sequence of a group consisting of amino acid sequences represented by any of 〇3, 105, 106, 1〇7, 1〇9, in, 113, 115, 116 and its homologs An amino acid sequence selected from the group consisting of amino acid sequences represented by any one of Sequence IDs 60, 63, 96, 1 and its homologs. 5. The method of any one of claims 1-4, wherein the biological 201033370 catalyst comprises an enzyme selected from the group consisting of an organism of the group consisting of: Escherichia ( Especially for E. coli), Vibrio, Bacillus (B. subtilis), Clostridium (Special Kluyveromyces) C. kluyveri), acetobutylicum, and C. perfringens, Streptomycetes (especially S. coelicolor) S. avermitilis, Pseudomonas (especially P_ putida and p. aeruginosa), Schwannella (Shewanella), Xanthomonas, Xylella, Yersinia, Treponema (especially T. denticola), Eubacterium (Eubacterium) (especially E. pyruvativorans), Microscilla (Special Microscilla marina, Aeromonas (especially Aeromonas hydrophila), Megasphaera (especially M. aeruginosa (M. Elsdenii)), Acinetobacter, Deinococcus (especially D. radiodurans), Yarrowia (especially Yarrowia) Lypolytica)), Euglenozoa (especially Euglena gracilis); Saccharomyces (especially S. cerevisiae), Kluyveromyces (especially K. lactis, Schizosaccharomyces (especially s. pombe), Candida (especially Candida tropicalis) C. tropicalis)), Aspergillus (especially for a. niger and A. nidulans), Penicillium (especially for P. chrysogenum) )), Arabidopsis (especially for Arabidin) A. thaliana)), Homo sapiens, Rattus norvegicus, Bos taurus, Cavia sp., Caenorhabditis elegans and Drosophila The method of any one of claims 1-5, wherein the 2,3-dehydroadipate or the 2,3-dehydroadipate thioacetate is converted to 3. Preparation of adipate or 3-hydroxyadipate thioester. 7. The method of claim 6, wherein the 3-adipate or thioester is capable of catalyzing the dehydration of 3-hydroxydecyl ester or 3-hydroxyindole thioester to 2-enyl oxime or sulfur One of the ester biocatalysts preferably comprises a group selected from the group consisting of dehydratase 0 (11*〇17386) (£4.2.1.17), preferably selected from the enol-CoA hydratase. (hydratase) (EC 4.2.1.17), 3-butyroside-CoA dehydratase (EC 4·2· 1.55) and long-chain olefin-CoA hydratase (EC 4.2.1.74) One of the enzymes is converted by biocatalysis in the presence of a biocatalyst. 8. The method of claim 7, wherein the biocatalyst comprises an enzyme selected from one of the group consisting of: Acinetobacter (particularly Acinetobacter sp. ADP1 and A. calcoaceticus, Acetobacter sp. 3 201033370 (Alcaligenes) (especially Alcaligenes facilis D2), Aspergillus (especially A. niger), denitrification Arzoarcus (especially A. evansii), Azomonas, Azotobacter, Bacillus (especially Bacillus halophila) (B. halodurans)), Corynebacterium (especially C. glutamicum and C. aurantiacum), E. coli, Flavobacterium (E. coli) Flavobacterium), Neurospora (especially N. crassa), Penicillium (especially P. chrysogenum), Pseudomonas (special) For P. putida and fluorescent fakes P. fluorescens), Rhodopseudomonas (especially R. palustris), Rodococcus (especially Rhodococcus sp. RHA1), Aeromonas (specially A. caviae), Clostridium (special acetobutylicum), Gossypium (special) (G. hirsutum), Rhodospirillum (R. rubrumi) and Ralstonia (Special Nutritional Russellium (R) Eutropha)), Euglenozoa (especially Euglena gracilis), Megasphaera (especially M. elsdenii), and Saccharomyces (Saccharomyces) In particular, it is S. cerevisiae, and mammals (especially European babies (b〇s taurus), human (Homo sapiens), brown rats (Rattus norvegicus), and 4 201033370 Sus scrofa). 9. The method of any one of clauses 6, 7, or 8 wherein the 3-adipate or thioester is converted to a 3-keto adipate or a hexanoic acid. Sulfur is prepared for the purpose of sulfur. 10. The method of claim 9, comprising the biocatalytic conversion of the 3-keto adipate or 3-ketoadipate thioester in the presence of a biocatalyst, the biocatalyst being particularly capable of catalyzing the reduction of a carbonyl group Alcohol-based or biocatalyst which can catalyze the reduction of 3-brewed or 3-ketosulfonyl ester to the corresponding 3-hydroxy oxime ester or thioester, and is selected from dehydrogenase (rc· 1.1.1). The group consisting of is preferably selected from the group consisting of 3-hydroxyindole-CoA dehydrogenase (EC 1.1.1.35 and EC 1.1.1.36), 3-hydroxybutyrene-CoA dehydrogenase (EC 1.1.1.157). , a group consisting of 3-hydroxyheptane-CoA dehydrogenase (EC 1.1.1.259) and long-chain 3-hydroxyindole-CoA dehydrogenase (EC 1·1·1·211). 11. The method of claim 1, wherein the biocatalyst comprises an enzyme selected from the group consisting of an organism of the group consisting of: Acinetobacter (particularly Acinetobacter sp. ADP1 and A. calcoaceticus, Alcaligenes (especially Alcaligenes D2 and A. eutrophus), Arzoarcus (Special clothing) A. evansii), Bacillus (especially B. halodurans), Bordetella (especially B. pertussis, Bo Burkholderia (Special B. pseudomallei and B. xenovorans) Corynebacterium (especially C. glutamicum, C. aurantiacum and effective C. efficiens), Deinococcus (especially D. radiodurans), E. coli, Flavobacterium, Cray Klebsiella (special K. pneumonia), Pseudomonas (particularly P. putida and p. fluorescens), Rhodopseudomonas Rhodopseudomonas (particularly referred to as R. palustris), Rodococcus (especially R. erythropolis, Rhodococcus rhodochrous (R. r 〇 Pacus) And Rhodococcus sp. RHA1), Aspergillus (especially A. niger), Neurospora (especially rough red 'N. crassa), Penicillium (peniciiiiUm) (especially for P. chrysogenum), Saccharomyces, Bos taurus, Rattus norvegicus, Sus scrofa, Homo sapiens, shuttle Clostridium (especially acetylbutyric acid # Clostridium bsctobutylicunO and c. kluyveri), Euglenozoa (especially Euglena gracilis), Megacoccal ( Megasphaera) (especially for Melsdenii), Ralstonia (Special Nutrition Russell) R. eutropha), and fungal micelles (z〇〇gl〇ea) (especially Zoogloea ramigera) ° 12. As claimed in the scope of patents 9' 1〇, or n A method according to any one, wherein the 3-ketoadipate or 3-ketoadipate thioate is prepared by reacting succinate or succinate with thioacetate or thioacetate. 6 201033370 13. The method of claim 12, comprising reacting a succinate or a thioester with an acetate or a thioester by biocatalysis in the presence of a biocatalyst, the biocatalyst preferably being a biocatalyst Included from the group consisting of a switchase (EC 2.3.1), preferably selected from the group consisting of acetamidine-CoA: acetamidine-CoAC-acetyltransferase (EC 2 3 (9), 醯_c〇a : B---(: 〇八(:-transferase €€2.3.1.16) and butyl bismuth-(: 〇8: acetamidine-CoA C-butadiene transferase (EC 2.3.1.174 An enzyme capable of transferring an ethylene group in a group consisting more particularly an enzyme comprising an amino acid sequence recognized by any one of the sequence IDs 13 or a homolog thereof. The method of item 12 or 13, wherein the biocatalyst comprises an enzyme selected from the group consisting of an organism of the genus Acinetobacter (particularly Acinetobacter sp. ADP1 and Acinetobacter acetate) A. calcoaceticus)), Agrobacterium (especially A. tumefaciens), Alcaligenes (Alc) Aligenes) (especially for Alcaligenes sp. D2 and A. eutrophus), Arthrobacter, Arzoarcus (a. evansii) ), Azomonas, Azotobacter, Bacillus (especially B. halodurans), Beijerinckia, slow Bradyrhizobium, Burkholderia, Clostridium (especially C. kluyveri, acetobutylicum, Bayer Link) Clostridium (C. beijerinckii), Comamonas, Corynebacterium 7 201033370 V (Corynebacterium) (especially C. glutamicum ' and C. aurantiacum )), E. coli, Enterobacter, Flavobacterium, Megasphaera (especially for the genus Escherichia coli (μ. elsdenii), Nocardia) (Norcadia), Pseudomonas (especially Platts) Pseudomonas (p. pUtida), P. aeruginosa and Pseudomonas species B13), Ralstonia (especially R. serrata (especially r· eutropha)), Rhizobium, Rhodopseudomonas (special, other > R. palustris), Rodococcus (Rodococcus) Especially for Rhodococcus rhodochrous (R. erythr0p〇lis), Rhodococcus erythropolis (R. opacus) and Rhodococcus species RHA1), Aspergillus (especially for a. niger) , Euglenozoa (especially Euglena gracilis), Neurospora (especially for rare red mold (n. crassa), Penicillium genus (Penicillium X especially for Penicillium chrysogenum) Chrysogenum)), Rhodotorula, Saccharomyces, Trichosporon (especially t. cutaneum) 〇 15. For patent scopes 1-14 A method according to any one of the preceding claims, wherein any one of the esters is selected from the group consisting of biologically active groups, in particular selected from Coenzyme A, Acid ubiquinone can be bound to a thiol or a peptide carrier protein, N-acetamidine-cysteamine, methyl-thio-glycolic acid, methyl-hydrazine-propionic acid, ethyl-hydrazine- A group consisting of propionic acid, decyl-indole-butyric acid, sulfhydryl-hydrazine-butyric acid, and propionic acid. 8 201033370 16. A method for preparing adipic acid 'comprising a method for preparing adipic acid lysate or adipic acid sulphur lysate according to the method of any one of claims 1 to 15 and hydrolyzing The distillate of the first to the first term of the item - the obtained adipic acid vinegar or adipic acid sulphur vinegar, wherein the hydrolysis is preferably catalyzed by a biocatalyst, in particular comprising One of the enzymes in the group consisting of hydrolase (EC 3.1.2) is catalyzed by a biocatalyst. A method for preparing adipic acid, which comprises preparing adipic acid or adipic acid thioacetate in a method according to any one of claims 1.15, and transferring it as in the scope of claim 1 to 15 The active group of adipate or thiodicarboxylate obtained by the method of any one of the preceding claims, wherein the active group, the transfer is catalyzed by a biocatalyst, preferably an enzyme comprising a catalyzed transfer of a sulfur-containing V group The biocatalyst (EC 2.8) is more preferably a biocatalyst selected from the group consisting of C〇A transferase (EC 2, 8.3). 18. A method for the preparation of 5 - valeric acid, which comprises the method of preparing adipic acid ester or adipic acid thioester in the method of any one of claims 1 to 15 or as claimed in the patent application. A method according to the 16th or 17th aspect, wherein adipic acid is prepared, and the monoacid acid, the monothiocyanate or adipic acid is converted into 5-methylvaleric acid. 19. The method of claim 18, wherein the conversion to 5 mevalonate is catalyzed by a biocatalyst, preferably the biocatalyst comprises a group selected from the group consisting of oxidoreductases (EC 1.2.1). One of the enzymes in the population, preferably selected from the group consisting of disc dehydrogenase (EC 1.2.1.3, EC 1.2.1.4 and EC 1.2.1.5), malonate semialdehyde dehydrogenase (EC 12115), succinic acid Semi-over 9 201033370 dehydrogenase (EC 1.2.1.16 and EC 1.2.1.24); glutamic acid semialdehyde dehydrogenase (EC 1.2.1.20), amine adipic acid semialdehyde dehydrogenase (EC 1.2.1.31), a group consisting of adipic acid semialdehyde dehydrogenase (EC 1.2.1.63); or selected from a dehydrogenase (acetylation) (EC 1.2.1.10), adipose 醯-CoA reductase (EC) 1.2.1.42), a group consisting of long-chain fat 醯_coA reductase (EC 1.2.1.50), butadiene dehydrogenase (ec 1.2.1.57) and succinic acid semi-dehydrogenase (acetylation) . 20. A process for the preparation of 6-aminocaproic acid, which comprises the process of preparing 5-methylvaleric acid and converting the 5-carboxalic acid to 6-amine in the process of claim 18 or 19. acid. 21. The method of claim 20, wherein the conversion is catalyzed by a biocatalyst to a catalytically transaminated and/or reductive aminated biocatalyst, preferably comprising a transaminase selected from One of the enzymes in the group consisting of EC HI) and aminopyridinium dehydrogenase (EC 1.4.1), more preferably selected from the group consisting of 10,000-aminoisobutyric acid: Transaminase, alanine transaminase, aspartate transaminase, 4-amine-butyrate transaminase (EC 2.6.19), L_ lysine 6 transaminase]%), 2_amine Acid to amino acid (EC 2. (1) 9), 5 • aminic acid transaminase (Μ 2.6丄48), 2-aminohexanoic acid transamin (EC 2.6丄67), lysine: pyruvate 6-turn - Saki-Biocatalyst in the group consisting of amines such as hydrazine and lysine _6 deazaase 1A1.18). 22•If you apply for the _2Q cut-off method, one of them contains the homologue according to sequence _, sequence touch 83, sequence ID84, sequence 4, sequence ID 136 sequence 138 or any of these sequences. 201033370 A transaminase of an amino acid sequence. 23. The method of claim 20, 21 or 22, wherein the biocatalyst comprises from a genus Vibri(R); Pseudomonas; BaciUus; Mericurialis, ASpienium; Ceratonia; mammal; Neur〇sp〇m; Escherichia; Thermus (Thermus); Saccharomyces; Brevibacterium; Corynebacterium; Proteus (pmteus); Agrobacterium; Ge〇badllus; Acinetobacter (Acinetobacter); an organism-one enzyme in a group consisting of Ralst〇nia and Salmonella. A method for producing caprolactam, which comprises the process of preparing 6-aminohexanoic acid by the method of any one of claims 20-23, and cyclizing the 6-aminocaproic acid, thereby forming a Endoamine. 25. A host cell comprising any one of claims 2, 3, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 21, 22 and 23 The defined plum or nucleic acid sequence preferably comprises a respective coding as in the scope of claims 2, 3, 4, 5, 7, 8, 10, 13, 14, 16, 17 ' 19, 21, 22 and At least two nucleic acid sequences of different enzymes as defined in any of the 23 items. 26. The host cell of claim 25, comprising the sequence id 42-67, sequence ID 74-81, 94, 96, 98, 100, 102, 103, 1〇5, 107, 109, 111, 113, 115, 116, or a homologous nucleic acid sequence thereof, represented by 11 201033370. 27. The method of claim 24, comprising contacting a cell comprising a biocatalyst with a fermentable carbon source, the biocatalyst comprising a host cell or a different organism as claimed in claim 25 or 26; The catalyst 'the side of the towel' contains any of the compounds which will be (4) converted to the compound to be prepared, or the compound in which the cells are prepared to be converted to the compound to be prepared from the carbon source. 28·- a method for preparing adipic acid from succinic acid or succinic acid or sulphuric acid, through a plurality of reactions, as required, according to claim 16 or 17 of the patent application, wherein the reaction At least - by the use of biocatalyst catalysis. 29. The method of claim 28, comprising (1) 1⁄4 for succinic acid or sulphuric acid and reacting the sulphur or sulphuric acid with vinegar or thioester to form 3-keto adipate Or a thioester; (2) hydrogenating the 3-keto adipate or the 3-keto group of the thioester to form 3_ adipic acid vinegar or sulphuric acid; (3) the 3-hydroxyadipate or Dehydrogenation of the thioester thereby forming a 2-3-dehydroadipate or a thioester; (4) hydrogenating the c-c double bond of the 2,3-dehydroadipate or thioester, thereby forming an adipate Or a thioester; and (5) hydrolyzing the ester bond or thioester bond, thereby forming adipic acid. A multi-peptide comprising an amino acid sequence according to any one of Sequence IDs 57, 68, 69, 70, 71, 72, 79, 85 and homologs thereof. A polynucleotide encoding a multi-peptide of the third aspect of the patent application. 12