201029569 六、發明說明： 【發明所屬之技術領域】 本發明係有關一種改良水稻生長之方法。更明確言之，係有關一種 藉由使用特定化合物處理，於還原之土壤中改良水稻生長之方法。 【先前技術】 已知下列化合物具有農藥效應：說明於EP00192060A1中之益達 胺(imidacloprid)、說明於 EP 00376279 A1 中之可尼丁(clothianidin)、說 ❹明於 EP 00235725 A1 中之赛果培(thiacloprid)、說明於 EP 00580553 A1 中之賽速安(thiamethoxam)、說明於EP 00649845 A1中之達特南 (dinotefUmn)、說明於 EP 00295117 A1 中之烯蟲靈(nitenpyram)、說明 於 WO 1"7/〇22593Α1 中之乙蟲清(ethiprole)與說明於 EP00295117A1 中之芬普尼(fipronil)。此外，曰本專利公告案案號2〇〇3_527329、曰本 專利公告案案號2007-536917與日本專利公告案案號2003-537176中 已說明類新菸鹼類對植物生長之影響。在日本專利申請公開公告案案 號2007-269803中，說明芳基吡唑類對植物生長之控制效應。201029569 VI. Description of the Invention: [Technical Field of the Invention] The present invention relates to a method for improving rice growth. More specifically, it relates to a method for improving rice growth in reduced soil by treatment with a specific compound. [Prior Art] The following compounds are known to have a pesticidal effect: the imidacloprid described in EP00192060A1, the clothianidin described in EP 00376279 A1, and the fruit culture described in EP 00235725 A1. (thiacloprid), thiamethoxam as described in EP 00580553 A1, dinotefUmn described in EP 00649845 A1, nitenpyram described in EP 00295117 A1, illustrated in WO 1" ; ethiprole in 7/〇22593Α1 and fipronil as described in EP00295117A1. In addition, the effect of neonicotinoids on plant growth has been described in the patent publication No. 2〇〇3_527329, the Japanese Patent Publication No. 2007-536917, and the Japanese Patent Publication No. 2003-537176. The control effect of arylpyrazoles on plant growth is illustrated in Japanese Patent Application Publication No. 2007-269803.
口水稻田中出現還原之土壤長久以來即為造成傷害稻植物嚴重生長 相害之問題。還原之土壤意指當水稻田土壤中歧大量未分解之有機 質時，微生物在供氧从下進行分解，結果土壌因氧氣雜而呈還原 、態。藉由測定氧化-還原電位’土壤之還原程度分成強還原態(e =約 -00mV ’無雜草，土壤呈黑色或偏藍色)或弱還原態氏=約·⑽， =些雜草）。若呈強還·時，赚部之垂直方向生長受到顯著抑 至可其依水田表面平行方向水平延伸。然而，根部無法生長 面平長i程度’有時候植物會枯萎死亡。此外，依水田表 千行方向水平延伸之根部會與常用於水稻田之除草劑之化學物質接 201029569 觸’在水田土壤表面上形成—層。因此即由除草劑產生毒性效應。同 時’很容易在曰本每個地區發現還原之土壤。亦即，由於溫暖地區水 稻栽種後之第二齡物殘留，直騎―季水稻插辨仍尚未分解。在 其他地區’燃燒鮮與米糠，科_稻作後之_物之作法已因社 會看法改變而日漸困難，因此在新—季開始插秩時仍 中。結果㈣魏㈣权土壤。目前處輯發生_土壤 控制水稻©巾的水’等等’但仍需要解決問題的根本辦法。 本發明者已發現類新於驗類或芳基吼0坐類可改善還原之土壤中之 水^生長，因而完成本發明。因此本發明提供—種改良還原之土壤中❹ 水稻生長之方法’其係在獅巾制類祕_或 【發明内容】 本發明提供一種 二2 2壤中改良水稻生長之力法其係在稻田中施加績於 ()=據第(1)項之方法，其中該賴於驗係選自下列任一者：益達 ❹ 心達特南與稀蟲靈。 ⑷減其中方基㈣為乙蟲清或芬普尼。 秧苗之育苗法其中該類新菸鹼或芳基吡唑類係施加至水稻 苗的鬥^^之方法’其中該麵於驗或芳基°比哇類係在雜稻缺 «的冋時或之後施加至稻田中。 加子其中該類新於驗或芳基°比°坐類係在播種前施 了用於本發明之類新終驗代表性實例包括：1.氣-3-吼咬基)甲 201029569 基]_Ν·硝基·2_咪嗤咬亞胺(俗名：益達胺)、Ν·[(2_氣_5寺坐基)甲基]_象 -曱基-N”-硝基-脈（俗名：可尼丁）、[3|氯>比咬基)甲基]·2•亞嗟唾 咬基]-氰醯胺(俗名：賽果培）、3-[(2-氣冰三唑基)甲基]四氫_5_甲基·Ν 硝基-4Η-υ»井-4-亞胺(俗名：赛速安）、Ν_甲基.石肖基机[(四 氫！咬喃基)曱基]-脈（俗名：埃特南㈣沖修氯各吼咬基)甲基他 乙基-Ν’-曱基-2-石肖基-U-乙二胺(俗名：缚蟲靈），等等。較佳化合物為 益達胺。 " ❹ 同樣地’芳基吼销之代表性實例包括5_胺基-1-[2,6_二氣_4_(三氟 曱基)-笨基]_4_[(二氟曱基)-亞確醯基]_1H_nfc〇甲猜(俗名：芬普尼）、 5-胺基-1-[2,6-二氯-4-(三氟曱基)苯基Μ·(乙炔基亞俩基>1H_m 甲腈(俗名：乙蟲清），等等。 為了進行本發财法，類祕_鱗基姑_在插稻秩苗的同 時或之後施加至稻田中，或預先施加至培育水稻缺苗之育苗箱中 用於前處理稻植物種子。 當本發明方法使用類新终驗時，其有效量係每公頃水稻田施用約 10至1000克之範圍内，每箱育苗箱(3〇cm x 6〇cm X 3em)施用約〇 1至 10克之範圍内’及每1公斤需進行種子處理之種子施用約〇2至 克。气使用芳,嗤類時，其有效量係每公頃水稻田施用約10至議 克之範圍内，每箱育苗箱(30cm x 6〇cm X 3cm)施用約0.1至10克之範 圍内，及每1公斤需進行種子處理之種子施用約〇2至2〇克。 本發明絲所使用類新級類或芳基 < 销可製成—般調配物型 式。此等調配物型式包括例如：液體、乳液、可濕化散劑、製成粒之 可濕化散劑、懸浮液、粒劑調配物及種子之包衣劑，等等。 此等調配物可依據本身已知方法製備。例如·可由活性化合物(類 201029569 新^絲基料)與朗_ 固態稀釋劑或載劑，及稀釋劑或載劑， 散劑混合，製她物。編活_，嫩化劑及/或分 當使用水作為展開劑(補充劑)時， 助溶劑。 亦可使用例如：有機溶劑作為辅 液態稀釋劑或載劑包括例如：芳香 基酮甲基Zr醇其甘醇及其_與鋪）、_(例如·· _、甲基乙 異基酮、環己酮，物、高極性溶麵如：二甲基甲 醯、一甲亞石風，等等）、水，等等。 固態稀釋劑包括例如：天然礦物磨粉(例如：高嶺土、黏土、滑石、 如要ΐ二=、矽鎂土、蒙脫土與矽藻土，等等)，與合成礦物磨粉(例 如.间刀散性矽酸、礬土、矽酸鹽，等等），等等。 用於粒劑調配物之固態載劑包括例如：粉化與過筛之礦石(例如： 人Ξΐ、大理石、浮石、海泡石、白雲石’等等）、無機或有機粉末之 η成粒子及有機材料之細粒(例如：鋸屑、椰子殼、 等等），等等。 乳化劑包括例如：非離子性與陰離子性乳化罐物：聚氧乙稀脂 肪酸醋、聚氧乙烯脂肪醇鍵(例如：絲芳基聚二_)、雜炫基醋、 硫酸烷基酯與磺酸芳基酯，等等，與白蛋白之水解產物，等等。 分散劑包括例如··木質素亞硫酸鹽廢液與甲基纖維素。 調配物(粒刺調配物，等等)亦可使用固定劑(或增麵），其實例包 括幾曱基纖維素、天然或合成聚合物(例如：阿拉轉、聚乙稀醇與聚 ❹ 〇 玉米穗轴、煙草桿 201029569 乙酸乙稀酯，等等），等等。 亦可使用著㈣’其實例包括無航素(例如：氧化鐵、氧化鈦、The appearance of reduced soil in the rice field has long been a problem that causes serious damage to the damaged rice plants. The reduced soil means that when a large amount of undecomposed organic matter is found in the soil of the paddy field, the microorganisms are decomposed under oxygen supply, and as a result, the soil is reduced by oxygen. By measuring the degree of reduction of the oxidation-reduction potential' soil, it is divided into a strong reduction state (e = about -00mV 'no weed, soil is black or blue) or weak reduction = about · (10), = some weeds) . If it is strong, the growth in the vertical direction of the earning section is significantly inhibited to extend horizontally in parallel with the surface of the paddy field. However, the roots cannot grow, and the surface length is i. Sometimes plants will wither and die. In addition, the roots extending horizontally in the direction of the thousand rows of the paddy field will be connected to the chemical substances commonly used in herbicides in paddy fields. Therefore, the toxic effect is produced by the herbicide. At the same time, it is easy to find the restored soil in each area of Sakamoto. That is to say, due to the residual second-generation of the rice planted in the warm area, the direct riding-season rice has not yet been decomposed. In other regions, the practice of burning fresh and rice bran, the _ after the rice crop has become increasingly difficult due to changes in social perceptions, so it is still in the middle of the new season. Results (4) Wei (four) right soil. At present, the occurrence of _ soil control rice, towel water, etc., still requires a fundamental solution to the problem. The present inventors have found that the novel or the aryl quinone can improve the growth of water in the reduced soil, thus completing the present invention. Therefore, the present invention provides a method for improving the growth of rice in the soil of the reduced soil, which is based on the lion towel system or the content of the invention. The present invention provides a method for improving the growth of rice in the soil. The performance is based on () = the method according to item (1), wherein the dependent system is selected from any one of the following: Yida ❹ 达 特 特 与 与 与 与 与. (4) Subtracting the square base (4) is Bifido or Fenpney. The seedling raising method, wherein the neonicotinoid or arylpyrazole is applied to the rice seedlings, wherein the surface is inspected or the aryl group is lower than the wow line in the rice It is then applied to the rice fields. Additives in which the new test or aryl ratio is applied before planting, representative examples of new final tests for use in the present invention include: 1. gas-3-吼 base) A 201029569 base] _Ν·Nitro·2_Mimmet bite imine (common name: etadamine), Ν·[(2_气_5寺坐基)methyl]_象-曱基-N"-nitro-pulse Common name: Cotinidine), [3|Chlorine>Bit base)Methyl]·2•Athene sputum base]-Cyanopyramine (common name: Sai Pei Pei), 3-[(2-气冰三Azolyl)methyl]tetrahydro_5_methyl·Ν nitro-4Η-υ» well-4-imine (common name: Sai Suan), Ν_methyl. Shi Xiaoji machine [(tetrahydro! Base) 曱基]-脉 (common name: Etnam (four) washed chlorine each bite base) methyl t-ethyl-Ν'-mercapto-2-stone Schottky-U-ethylenediamine (common name: Bianling) , etc. The preferred compound is edaramin. " ❹ A similar example of 'aryl-based credits' includes 5-amino-1-[2,6-diox_4_(trifluoromethyl)- Stupid base]_4_[(difluorodecyl)-arsenyl]_1H_nfc 〇甲猜 (common name: Fenpney), 5-amino-1-[2,6-dichloro-4-(trifluoromethane) Phenyl hydrazone (ethynyl arylene) > 1H_m carbonitrile (common name: acetaminophen), etc. In order to carry out the present financing method, the class secret _ scale base _ is applied to the paddy field at the same time as or after the insertion of rice seedlings, or pre-applied to the seedling box for cultivating rice seedlings for pretreatment of rice plant seeds. When the method of the present invention uses a new type of final test, the effective amount is in the range of about 10 to 1000 grams per hectare of paddy field, and about 1 to 10 grams per box of seedling box (3 〇 cm x 6 〇 cm X 3em) is applied. Within the 'and every 1 kg of seeds to be seed treated, about 2 to gram. When using aryl, hydrazine, the effective amount is about 10 to the range of rice per hectare of rice field, each box of seedling boxes (30cm) x 6〇cm X 3cm) is applied in the range of about 0.1 to 10 grams, and about 2 to 2 grams per 1 kilogram of seeds to be seed treated. The novel class or aryl group used in the yarn of the present invention < pin It can be formulated as a general formulation. Examples of such formulations include liquids, emulsions, wettable powders, wettable powders, suspensions, granule formulations and seed coatings, etc. Etc. These formulations can be prepared according to methods known per se. For example, Compound (class 201029569 new silk base) and lang _ solid diluent or carrier, and diluent or carrier, powder mixture, make her. Edit _, tenderizer and / or use water as a When the developer (supplement) is used as a co-solvent, it is also possible to use, for example, an organic solvent as a secondary liquid diluent or a carrier, for example, an aryl ketone methyl Zr alcohol, a glycol thereof, and a platoon thereof, _ (for example · _, methyl ethyl ketone, cyclohexanone, high polarity soluble surface such as: dimethylformamidine, ketone, etc.), water, and so on. Solid diluents include, for example, natural mineral milling (eg, kaolin, clay, talc, bismuth, strontium, montmorillonite, and diatomaceous earth, etc.), and synthetic mineral milling (eg, between Knives of tannic acid, bauxite, citrate, etc.), and so on. Solid carriers for granule formulations include, for example, powdered and sieved ores (eg, human enamel, marble, pumice, sepiolite, dolomite, etc.), η-forming particles of inorganic or organic powders, and Fine particles of organic materials (eg sawdust, coconut shell, etc.), etc. Emulsifiers include, for example, nonionic and anionic emulsion cans: polyoxyethylene fatty acid vinegar, polyoxyethylene fatty alcohol linkages (eg, silk aryl poly-), rhodamine vinegar, alkyl sulfate and sulfonate An aryl aryl ester, etc., a hydrolysis product with albumin, and the like. Dispersing agents include, for example, lignin sulfite waste liquid and methyl cellulose. Fixatives (grain formulations, etc.) may also be used as fixatives (or facets), examples of which include several fluorenyl celluloses, natural or synthetic polymers (eg, Ala-trans, polyethylene, and polyfluorene). Corn cob, tobacco rod 201029569 vinyl acetate, etc.), and so on. It can also be used (4), examples of which include aerospace (eg iron oxide, titanium oxide,
青染料與微量it素’如：鐵、猛、硼、銅、銘、鉬與鋅鹽，等等。 調配物可包含上述活性成份，其含量為〇1_95%重量比，較佳為 0.5-90%重量比。 ’ 本發明中，類新菸鹼或芳基吡唑類可以混合併用其他活性化合物， 如：農藥、消毒劑與除草劑，等等。 下文中’本發明參考下列實例更詳細·。然而，本發_ 受 其限制。 【實施方式】 實例 生物試驗與調配物實例 試驗化合物 H(6-氣定基)甲基]_N_硝基_2-咪麵亞俗名Cyan dyes and trace amounts of itinia such as: iron, fierce, boron, copper, Ming, molybdenum and zinc salts, and so on. The formulation may comprise the above active ingredient in an amount of from 1 to 95% by weight, preferably from 0.5 to 90% by weight. In the present invention, neonicotinoid or arylpyrazole may be mixed and used with other active compounds such as pesticides, disinfectants and herbicides, and the like. Hereinafter, the present invention is more detailed with reference to the following examples. However, this issue is limited by it. [Examples] Examples Biological test and formulation examples Test compound H(6-gas-based) methyl]_N_nitro-2-mimface common name
....... 取95份黏土礦物粒子(直徑分佈在〇 2 至2mm之範圍内)加至旋轉....... Take 95 parts of clay mineral particles (diameter in the range of 〇 2 to 2mm) to rotate
添加殿粉至得自水稻田之乾土壤中， 同時添加水(每1升乾土壤添 201029569 加3 ml澱粉）。所得土壤保持在室溫下ι〇天，測定氧化_還原電位。結 果證實出現強還原態。為了轉變成溫和還原態，進一步添加上述土^ 進行修改。 試驗實例1 :於強還原條件下測定對作物生長之改良效應之試驗 方法 取2.1葉期稻植物之缺苗(Koshihikari)移植至已填充如上述製備之 水稻田土壤(約-25〇mv)之1升塑膠盆中，並保持在溫室内，同時與該 移植同時施加試驗化學調配物。然後，慢慢對植物加水至深度2 cm， 於室溫下保持16天，以便檢視並比較稻植株之根部長度與地上部份之© 長度(共測量三次，取平均值）。分別以未處理及非還原條件下之稻植 株之根部與地上部份之生長速率定為100%，針對各化學調配物測定生 長率。結果示於下表1。 表1 處理組之化學 調配物 (200g/ha) 根部長度 (cm) 根部之生長 速率(％) 稻植株地上 部份之長度 (cm) 稻植株地上 部份之生長 速率(％) 未處理 非還原條件 10.0 100 38.0 100 未處理 1.5 15 16.0 42 益達胺 ---. 強還原條件 8.0 80 21.0 55 芬普尼 10.0 100 35.0 92Add the temple powder to the dried soil from the paddy field and add water (add 201030569 plus 3 ml of starch per liter of dry soil). The obtained soil was kept at room temperature for 1 day, and the oxidation-reduction potential was measured. As a result, a strong reduction state was confirmed. In order to convert to a mild reduced state, the above soil is further added for modification. Test Example 1: Test method for determining the effect of improvement on crop growth under strong reducing conditions. 2.1 Foliage of rice plants (Koshihikari) was transplanted to paddy soil (about -25 〇mv) which had been filled as described above. The 1 liter plastic pot was kept in the greenhouse while the test chemical formulation was applied simultaneously with the transplant. Then, slowly add water to the plant to a depth of 2 cm and keep it at room temperature for 16 days to examine and compare the root length of the rice plant with the length of the aerial part (measured three times, averaged). The growth rate of the root and aerial parts of the rice plants under untreated and non-reduced conditions was determined to be 100%, and the growth rate was determined for each chemical formulation. The results are shown in Table 1 below. Table 1 Chemical formulation of treatment group (200g/ha) Root length (cm) Root growth rate (%) Length of aboveground part of rice plant (cm) Growth rate of aboveground part of rice plant (%) Untreated non-reduced Conditions 10.0 100 38.0 100 Untreated 1.5 15 16.0 42 Idadine---. Strong reduction conditions 8.0 80 21.0 55 Fenpney 10.0 100 35.0 92
在強還原條件下，未處理之稻植株根部與地上部份之生長速率僅分 別為非還原條件下之15%與42%。㈣，以益達胺處理時，稻植株之 201029569 根部與地上部份之生長速率則分別提高至80%與55%。此外，以芬普 尼處理時，稻植株之根部與地上部份之生長速率則分別提高至1〇〇0/。 與 92%。 δ式驗實例2 .於溫和還原條件下測定對作物生長之改良效應之試驗 方法 於溫室中，取2.1葉期稻植物之秧苗(K〇shihikari)移植至已填充如 上述製備之水稻田土壤(約_250mv)之1升塑膠盆中，同時與該移植同 時施加試驗化學調配物。然後’慢慢對植物加水至深度2咖，於室溫 下保持19天’以便檢視並比較稻植株之根部長度與長出土壤表面之植 株部份之重量(共測量三次，取平均值）。分別以於非還原條件下之未 處理組之稻植株根部與地上部份之生長速率定為1〇〇%，針對各化學調 配物測定生長率。結果示於下表2。 表2 處理組之化學 調配物 (200g/ha) 根部長度 (cm) 根部生長速 率（％) 稻植株地上 部份之長度 (cm) 稻楂株地上 部份之生長 速率 未處理 非還原條件 17.5 100 4.8 丁、’。夕 — — 100 未處理 溫和還原條件 13.1 75 3.7 77 乙蟲清 14.5 83 4.3 90 相對於㈣雜件T，於溫㈣原餅下，未處雜之雖株根 與地上部份之生長料僅㈣為挪與W。_，以乙蟲清處理時， 稻植株根部與地上雜之生長料分職高至曝與9〇%。Under strong reducing conditions, the growth rates of the roots and aerial parts of untreated rice plants were only 15% and 42%, respectively, under non-reducing conditions. (4) When treated with edamine, the growth rate of roots and aboveground parts of rice plant 201029569 increased to 80% and 55%, respectively. In addition, when treated with Fenpini, the growth rate of the roots and aerial parts of rice plants increased to 1〇〇0/. With 92%. δ test example 2. Test method for determining the effect of improvement on crop growth under mild reducing conditions In a greenhouse, 2.1 seedlings of rice plants (K〇shihikari) were transplanted to paddy soil which had been filled as described above ( In a 1 liter plastic basin of approximately _250 mv), a test chemical formulation was applied simultaneously with the transplant. Then, slowly add water to the plant to a depth of 2 coffee for 19 days at room temperature to examine and compare the root length of the rice plant with the weight of the plant part growing out of the soil surface (measured three times, averaged). The growth rate of the root and aerial parts of the rice plants in the untreated group under non-reducing conditions was set at 1%, and the growth rate was measured for each chemical formulation. The results are shown in Table 2 below. Table 2 Chemical formulation of treatment group (200g/ha) Root length (cm) Root growth rate (%) Length of aboveground part of rice plant (cm) Growth rate of aboveground part of rice plantment untreated Non-reducing condition 17.5 100 4.8 Ding, '.夕—100 Untreated mild reduction conditions 13.1 75 3.7 77 Ethyl bromide 14.5 83 4.3 90 Relative to (iv) miscellaneous T, under the warm (iv) raw cake, the growth of the roots and the aboveground parts is only miscellaneous (4) For the move and W. _, when treated with acetaminophen, the growth of the roots of the rice plant and the ground material were divided to 9%.