TW201024274A - Biologically active extract from dendrobium plant, use thereof and process for preparing the same - Google Patents

Abstract

A substance having the following formula is provided. Wherein R1 and R2 are selected from a group consisting of α -Ara, β -Ara and β -Xyl. The substance has an effect of accelerating a phagocytosis of RPE.

Description

201024274 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a process for producing a biologically active plant extract and an invention relating to the extract, and more particularly to a use of a water-soluble organic solvent or a water-soluble organic A process for obtaining a plant extract from a mixture of a solvent and water. [Prior Art] As described in U.S. Patent No. 7,1,1,577, Dendrobium is known to be a valuable medicinal material for treating ophthalmic diseases. It can be found from previous research results that the sarcophagus is traditionally the most biologically active as a therapeutic plant of Dendrobium. However, according to recent trends, the sarcophagus with its curative effect is commonly known as Cflw/Additional//, and the whole plant has a curative effect. The sarcophagus is known as Herba Dendrobii. According to the disclosure of the above U.S. Patent, it is known that retinal pigment epithelium (RPE) cells are monolayer cells on the surface of the retina, located in Bruch's membrance and photoreceptors. Between (photoreceptors). Because RPE cells can effectively remove or transport toxic substances and metabolites in the choroid and retinal nerve layers, they also constitute a very important blood-retinal barrier. In addition, RPE cells have many functions, such as: absorption of light, swallowing of rod-shaped cells and cone outer segments (ROS), phagosome decomposition, and synthesis of extracellular matrix. (extracellular matrix) and melanin (melanin), drug 201024274 detoxification, the necessary materials for the regeneration of the outer segment of the photoreceptor, the storage and delivery of vitamin A, the synthesis of dextran (Rodopsin), the formation of the retina .....and many more. Since the normal swallowing effect of RPE cells is quite relevant for maintaining the health of the light receptor in the retina, once the function of swallowing is reduced, it will cause degeneration of the photoreceptor. Although rPe cells die or shed somewhere with age, aged RPE cells still have phagocytic capacity. The loss of rod cells is more than that of cone cells, which may cause vision loss and disease in the elderly. Therefore, the maintenance of RPE cell function is very important for the visual system. In the immune system, nitric oxide plays a defensive role and is toxic to cells; in the vascular system, nitric oxide is the so-called endothelial cell relaxing factor (EDRF); in the central nervous system, nitric oxide is The role of neurotransmitter (niurotransmitter) nitric oxide is released during the conversion of L-arginine to L-citrulline by nitric oxide synthase (NOS). Nitric oxide synthase has three isoforms, neuronal NOS, endothelial NOS and immunologic NOS. Among them, neuronal NOS and endothelial NOS are constitutive forms 'cNOS, whose activity is regulated by dance ion Ca++ and calmodulin. The concentration of nitric oxide released belongs to nM (10_9M) level, and immunologic NOS is inducible. Inducible form, referred to as iNOS, but its activity is not regulated by Ca++ and calmodulin. The concentration of released nitric oxide belongs to 201024274 (10_6M) grade. The genes of cNOS and iNOS are located on different chromosomes. It has been found that retinal neuron, RPE cells, axon cells, amacrine and ganglion cells, and muller cells in the retina have nitric oxide synthase (Nitric). The existence of oxide synthase; NOS), and so far, there is evidence that nitric oxide plays an important role in physiological and pathological conditions, and is closely related to the function of the eye. Φ It has been found that nitric oxide has the ability to control retinal blood flow under basal conditions or ischemia. Moreover, nitric oxide may have a degree of regulation of intravascular damage to the retina caused by diabetes. In addition, when retinal glial cells and RPE cells are stimulated by LPS, IFN-γ, and TNF-a, they will promote the expression of oxidative synthase, and then produce a large amount of nitric oxide, thereby knowing nitric oxide. In the case of inflammation or infection of the retina, it may play a role of defense and protection. ❹ However, as of now, the location and characteristics of cNOS in visual receptors are still unclear. 'There are literatures that have cN〇s/tongue in the visual receptor body'. Other literature points out that the photoreceptor is outside the photoreceptor. The outer segments have cNOS activity, and the released nitric oxide can regulate the conduction of light, the signal transmission of synapses, the blood flow of the retina under physiological conditions or ischemia. The activity of iNOS is also expressed in some cells of the retina 'such as RPE cells and Muller cells'. It has been pointed out that in cultured bovine retinal pigment epithelial cells 201024274, if IFN-γ, LPS, TNF-a A large amount of one emulsified nitrogen can be produced about 12 hours after the stimulation, and can last for at least 96 hours. The effect of cytokines on the iNOS activity of RPE cells is quite complicated. For example, LPS and IFN-7 or TNF-α stimulation are required to produce 'a large amount of nitric oxide, and bFGF has the effect of inhibiting N〇s. TGF-. Cold has a slight effect on promoting NOS. For human RPE cells, there must be Interleukin- annihilation to produce large amounts of no, and LPS has no effect. TGF-/3 can significantly inhibit the release of nitric oxide. ' The performance of 'iN〇S may be beneficial when infected by bacteria, and the released nitric oxide can kill invading microorganisms; on the contrary, in some cases too much nitrogen monoxide can cause autologous Autoimmune disease or septic sh〇ck. In 1994, for the first time, there was evidence that nitric oxide was involved in the inflammatory response of the fundus. The applicant found that iNOS inhibitors can block uveitis caused by endotoxin. On the other hand, treatment of RPE with IFN_r and LPS produces a large amount of nitric oxide, which is inhibited by aFGF and bFGF, and is indicated to inhibit the performance of iN〇s rather than the stability of iNOS mRNA (stability). Therefore, the applicant of this case speculated that FGF can protect RP E from damage caused by endotoxin or cytokines. This shows that iNOS also plays an immunomodulatory role in the retina. Common retinopathy includes diabetic retinopathy, proliferative retreatopathy, and age-related macular degeneration 201024274 (Aged-macular degeneration), and retinal lesions are all ophthalmic diseases. The most difficult to treat. Hyperglycemia accelerates the occurrence of glycation, producing highly glycated end products (AGEs), which are thought to be associated with various vascular and neurological complications caused by the end of diabetes. The formation of AGE is the aldehyde group or the ketone group of the reducing sugar and the primary amino acid of the protein, which is unstable by the action of nonenzymatic Schiff base, and then Amdori product is produced via Amadori rearrangement. It is known that non-enzymatic saccharification is an irreversible reaction and occurs mostly on proteins with a long half-life. Due to the formation of AGE, cross-linking of the protein will make the protein resistant to protease (pr〇tease), so AGE The accumulation is an aging mark. With the increase of age, the content of AGE in the pyramidal neurons, Bruch's membrane, and collagen also increased. The rate of non-enzymatic saccharification is a primary reaction, and the rate of reaction is determined by the concentration of reducing sugars and proteins. In general, diabetes has a higher blood sugar concentration than normal people, which accelerates the development of saccharification. It is known that diabetes accelerates atherosclerosis, kidney damage, vascular damage, neuropathy, retinopathy, and diabetes. People with moderate stroke are directly related to AGE. Diabetes causes red blood cells to accumulate, mainly because albumin is ablated to cause changes in its tertiary structure, so that it loses the anti-aggregation function of 201024274. In addition, studies have shown that kidney-induced kidney filaments The change in spheroid permeability is due to the saccharification of albumin rather than the glomerular basement membrane. And vinegared proteins increase their ability to penetrate the blood brain barrier. • AGE can be combined with receptors, eggs on the cell surface

White matter binding, currently known as scavenger receptors type 1 and type 2, AGE receptor (RAGE, the receptor for AGE), OST-48 (AGE-R1), 80K-H ❹ phosphoprotein (AGE-R2) and galectin-3 (AGE-R3). AGE receptors can be found in mononuclear spheres, macrophages, endothelial cells, glial cells, etc. When cells are activated by AGE, they often make extracellular matrix proteins, jk tubes. Increased expression of vascular adhesion molecules and growth factors, along with different cell types and messages, accompanied by chemotaxis, angiogenesis, oxidative stress 'cell proliferation Or programmed cell death. ® A highly glycated end product receptor that is known to be expressed in a variety of cells in the human brain to clear AGE. When this ability is lost, it causes accumulation of extracellular AGE and induces an inflammatory response in the central nervous system. AGE can increase the expression of retinal vascular endothelial growth factor and pDGF-cold in RPE cells. AGE plays an important role in the aging process, so it is important to use glycated albumin as a pathological model for new drug development. 201024274 HGF/SF (Hepatocyte growth factor) is the most important growth factor in the liver. It is composed of a 60 KDa heavy chain (a chain) and a 30 KDa light chain (汐chain) with a disulfide bond. Connected to each other. The newly synthesized HGF/SF in the body is prepro HGF/SF which is biologically active after being modified by the enzyme to become a heterodimeric form. HGF is a multifunctional growth factor that not only regulates growth in a variety of cells, but also plays an important role in tissue repair and organ regeneration. HGF is widely distributed in the body, with the largest liver output, and the rest are pancreas, thymus, blood, small intestine, placenta, etc. In addition, HGF/SF or HGF/SF receptors are also present in the secretions and tissues of the eye, such as tears, lacrimal glands, corneas, etc. The visible HGF/SF may have a regulatory role in the eye. Retinal pigment epithelial cells not only have HGF and HGF receptors (c_Met), but c_Met's tyrosine phosphorylation is consistently expressed, so HGF may be a self-retined pigment epithelial cell. Somatic growth factors' may be associated with retinal development, wound healing, and retinal angiogenesis. It can be seen from the above that RpE cells play a very important role in the regulation mechanism of the retina. That is to say, it is known that the Chinese medicinal herbs of the genus Dendrobium are persistent, inhibiting or inhibiting certain functions or regulatory mechanisms of RPE cells. More special = Dendrobium can enhance the ability of RpE cells to swallow, mono-oxidation = formation and gene expression of HGF. Dendrobium plants can inhibit the gene expression of bFGF, VEGF and TGF-11 201024274 yS in RPE cells under normal or ischemic conditions. Therefore, studies related to factors that enhance the activity of RPE cells are important for promoting human health. In past studies, it has been known that Dendrobium extract has the effect of accelerating RPE cell swallowing and increasing AGE degradation. In order to further control its efficacy in accelerating RPE swallowing, it is necessary to have a deeper understanding of Dendrobium extract. For the sake of the job, the applicant, based on the lack of prior art, was carefully tested and researched, and the spirit of perseverance was used to develop the "Huoshan Dendrobium extract and its preparation method".

SUMMARY OF THE INVENTION The present invention provides a composition comprising a substance having the structure of Formula One,

5 OH 0

Formula I wherein I and 112 are one of the same and different functional groups. According to the above concept, the composition has a physiological activity. . The panel is based on the above concept, wherein the composition comprises an effect of accelerating a swallowing action of retinal pigment epithelial cells. . According to the above concept, wherein the substance is selected from the group consisting of a celery glycoside 6,8-di-CaL-°tb arabinose (apigenin 12 201024274 GJ-Di-CaL-arabinopyranoside), a celery glycoside 6-CPD- Helgenin-6-CaL-β arabinose (apigenin 6-CpD-Xylopyranosyl-8-CaL-arabinopyranoside), a celery glycoside 6-CaL-β than pain arabinosyl-8-CpD- ° One of the apigenin 6-CaL·- Arabinopyranosyl-8-CP~D-xylopyranoside and a mixture thereof. The invention further provides a substance having the structure of the formula 1,

The OH Ci has a center and I selected from one of arabinose (a#), p-arabinose (β-Ara) and β-xylose (β·χγ1), respectively. According to the above concept, the substance has an effect of promoting retinal pigment cell activity. The invention further provides a substance having the structure of the formula 1, R2 8 4丨

OH 〇 13 201024274

Wherein 匕 and r2 are respectively selected from the following group: Ri (or ruler 2) R2 (or Ri) 1 α-arabinose (a-Ara) a-Ara 2 P_ xylose (β-Xyl) a-Ara 3 α -Ara β-Xyl 4 β-arabinose (β-Ara) β-Ara 5 a-Ara β-Ara 6 β-Ara a-Ara 7 β-Xyl β-Ara 8 β-Ara β-Xyl 9 c-6 - deoxy-xylo-hexos-4-ulosyl a-Ara 10 c-6-Deoxy-xylo-hexos-4-ulosyl β-Ara 11 c- 6-Deoxy-xylo-hexos-4-ulosyl β-Xyl 12 β-Xyl c-6-Deoxy-xylo-hexos-4-ulosyl 13 α-Ara c-6-Deoxy-xylo-hexos-4-ulosyl 14 β -Ara c-6-Deoxy-xylo-hexos-4-ulosyl 15 c-6-Deoxy-xylo-hexos-4-ulosyl c-6-Deoxy-xylo-hexos-4-ulosyl 16 α-Ara β-glucose ( β-Glu) 17 α-Ara β-galactose (β-Gal) 18 β-Ara β-Glu 19 β-Ara β-Gal 20 β-Glu α-Ara 21 β-Gal α-Ara 22 β-Glu β -Ara 201024274 23 β-Gal β-Ara 24 β-Xyl β-Glu 25 β-Xyl β-Gal 26 β-Glu β-Xyl 27 β-Gal β-Xyl 28 c-6-Deoxy-xylo-hexos-4 -ulosyl β-Glu 29 c-6-Deoxy-xylo-hexos-4-ulosyl β-Gal 30 β-Glu c-6-Deoxy-xylo-hexos-4-ulosyl 31 β-Gal c-6-Deoxy-xylo -hexos-4-ul Osyl 32 a-Ara a-rhamnose (a-Rha) 33 β-Ara a-Rha 34 a-Rha a-Ara 35 a-Rha β-Ara 36 β-Xyl a-Rha 37 a-Rha β-Xyl 38 c-6-Deoxy-xylo-hexos-4-ulosyl a-Rha 39 a-Rha c-6-Deoxy-xylo-hexos-4-ulosyl 40 β-Glu β-Glu 41 β-Glu β-Gal 42 β -Gal β-Glu 43 β-Gal β-Gal 44 β-Glu a-Rha 45 a-Rha β-Glu 46 β-Gal a-Rha 15 201024274 47 α-Rha β-Gal 48 α-Rha α-Rha 49 --ribose (β-Ribose) β-Ribose 50 β-Ribose α-Arabinose 51 β-Ribose β-Arabinose 52 β-Ribose β-Xylose 53 β-Ribose β-Threose (β-Lyxose) 54 β-Ribose β-Allose 55 β-Ribose β-Altrose 56 β-Ribose β-mannose (β-Mannose) 57 β-Ribose β-Guluose (β-Gulose) 58 β-Ribose β-Idose 59 β-Ribose β-Talose 60 β-Ribose β-Tagatose 61 β-Ribose β-Fructose (β-Fmctose) 62 β-Ribose β-Glucose 63 β-Ribose β-Galactose 64 β-Ribose α-Rhamnose 65 β-Ribose c-6-Deoxy-xylo-hexos-4-ulosyl 66 α-Arabinose β-Ribose 67 α-Arabinose β-Lyxose 68 α-Arabinose β-Allose 69 α-Arabinose β-Altrose 70 α-Arabinose β-Mannose 16 201024274 71 α-Arabinose β-Gulose 72 α-Arabinose β-Idose 73 α-Arabinose β-Talose 74 α-Arabinose β- Tagatose 75 α-Arabinose β-Fructose 76 β-Arabinose β-Ribose 77 β-Arabinose β-Lyxose 78 β-Arabinose β-Allose 79 β-Arabinose β-Altrose 80 β-Arabinose β-Mannose 81 β-Arabinose β-Gulose 82 β-Arabinose β-Idose 83 β-Arabinose β-Talose 84 β-Arabinose β-Tagatose 85 β-Arabinose β-Fmctose 86 β-Xylose β-Ribose 87 β-Xylose β-Lyxose 88 β-Xylose β-Allose 89 β-Xylose β-Altrose 90 β-Xylose β-Mannose 91 β-Xylose β-Gulose 92 β-Xylose β-Idose 93 β-Xylose β-Talose 94 β-Xylose β-Tagatose 17 201024274 95 β-Xylose β-Fmctose 96 β-Lyxose β-Ribose 97 β-Lyxose α-Arabinose 98 β-Lyxose β-Arabinose 99 β-Lyxose β-Xylose 100 β-Lyxose β-Lyxose 101 β-Lyxose β-Allose 102 β-Lyxose β-Altrose 103 β-Lyxose β-Mannose 104 β-Lyxose β-Gulose 105 β -Lyxose β-Idose 106 β-Lyxose β-Talose 107 β-Lyxose β-Tagatose 108 β-Lyxose β-Fmctose 109 β-Lyxose β-Glucose 110 β-Lyxose β-Galactose 111 β-Lyxose α-Rhamnose 112 β- Lyxose c-6-Deoxy-xylo-hexos-4-ulosyl 113 β-Allose β-Ribose 114 β-Allose α-Arabinose 115 β-Allose β-Arabinose 116 β-Allose β-Xylose 117 β-Allose β-Lyxose 118 β-Allose β-Allose 18 201024274 119 β-Allose β-Altrose 120 β-Allose β-Mannose 121 β-Allose β-Gulose 122 β-Allose β-Idose 123 β-Allose β-Talose 124 β-Allose β-Tagatose 125 β-Allose β-Fmctose 126 β-Allose β-Glucose 127 β-Allose β-Galactose 128 β-Allose α-Rhamnose 129 β-Allose c-6-Deoxy-xylo-hexos-4-ulosyl 130 β-Altrose β -Ribose 131 β-Altrose α-Arabinose 132 β-Altrose β-Arabinose 133 β-Altrose β-Xylose 134 β-Altrose β-Lyxose 135 β-Altrose β-Allose 136 β-Altrose β-Altrose 137 β-Altrose β- Mannose 138 β-Altrose β-Gulose 139 β-Altrose β-Idose 140 β-Altrose β-Talose 141 β-Altro Se β-Tagatose 142 β-Altrose β-Fmctose 19 201024274

143 β-Altrose β-Glucose 144 β-Altrose β-Galactose 145 β-Altrose a_Rhamnose 146 β-Altrose c-6-Deoxy-xylo-hexos-4-ulosyl 147 β-Mannose β-Ribose 148 β-Mannose α-Arabinose 149 β-Mannose β-Arabinose 150 β-Mannose β-Xylose 151 β-Mannose β-Lyxose 152 β-Mannose β-Allose 153 β-Mannose β-Altrose 154 β-Mannose β-Mannose 155 β-Mannose β-Gulose 156 β-Mannose β-Idose 157 β-Mannose β-Talose 158 β-Mannose β-Tagatose 159 β-Mannose β-Fructose 160 β-Mannose β-Glucose 161 β-Mannose β-Galactose 162 β-Mannose α-Rhamnose 163 β -Mannose c-6-Deoxy-xylo-hexos-4-ulosyl 164 β-Gulose β-Ribose 165 β-Gulose a-Arabinose 166 β-Gulose β-Arabinose 201024274 167 β-Gulose β-Xylose 168 β-Gulose β- Lyxose 169 β-Gulose β-Allose 170 β-Gulose β-Altrose 171 β-Gulose β-Mannose 172 β-Gulose β-Gulose 173 β-Gulose β-Idose 174 β-Gulose β-Talose 175 β-Gulose β-Tagatose 176 β-Gulose β-Fmctose 177 β-Gulose β-Glucose 178 β-Gulose β-Galact Os 179 β-Gulose α-Rhamnose 180 β-Gulose c-6-Deoxy-xylo-hexos-4-ulosyl 181 β-Idose β-Ribose 182 β-Idose α-Arabinose 183 β-Idose β-Arabinose 184 β-Idose β-Xylose 185 β-Idose β-Lyxose 186 β-Idose β-Allose 187 β-Idose β-Altrose 188 β-Idose β-Mannose 189 β-Idose β-Gulose 190 β-Idose β-Idose 21 201024274 191 β- Idose β-Talose 192 β-Idose β-Tagatose 193 β-Idose β-Fmctose 194 β-Idose β-Glucose 195 β-Idose β-Galactose 196 β-Idose α-Rhamnose 197 β-Idose c-6-Deoxy-xylo -hexos-4-ulosyl 198 β-Talose β-Ribose 199 β-Talose α-Arabinose 200 β-Talose β-Arabinose 201 β-Talose β-Xylose 202 β-Talose β-Lyxose 203 β-Talose β-Allose 204 β -Talose β-Altrose 205 β-Talose β-Mannose 206 β-Talose β-Gulose 207 β-Talose β-Idose 208 β-Talose β-Talose 209 β-Talose β-Tagatose 210 β-Talose β-Fmctose 211 β- Talose β-Glucose 212 β-Talose β-Galactose 213 β-Talose α-Rhamnose 214 β-Talose c-6-Deoxy-xylo-hexos-4-ulosyl 22 2010 24274 215 β-Tagatose β-Ribose 216 β-Tagatose α-Arabinose 217 β-Tagatose β-Arabinose 218 β-Tagatose β-Xylose 219 β-Tagatose β-Lyxose 220 β-Tagatose β-Allose 221 β-Tagatose β-Altrose 222 β-Tagatose β-Mannose 223 β-Tagatose β-Gulose 224 β-Tagatose β-Idose 225 β-Tagatose β-Talose 226 β-Tagatose β-Tagatose 227 β-Tagatose β-Fmctose 228 β-Tagatose β-Glucose 229 β-Tagatose β-Galactose 230 β-Tagatose α-Rhamnose 231 β-Tagatose c-6-Deoxy-xylo-hexos-4-ulosyl 232 β-Fmctose β-Ribose 233 β-Fmctose α-Arabinose 234 β-Fructose β- Arabinose 235 β-Fmctose β-Xylose 236 β-Fmctose β-Lyxose 237 β-Fructose β-Allose 238 β-Fmctose β-Altrose 23 201024274 239 β-Fmctose β-Mannose 240 β-Fmctose β-Gulose 241 β-Fmctose β -Idose 242 β-Fmctose β-Talose 243 β-Fmctose β-Tagatose 244 β-Fmctose β-Fmctose 245 β-Fmctose β-Glucose 246 β-Fmctose β-Galactose 247 β-Fmctose α-Rhamnose 248 β-Fmctose c- 6-Deoxy-xylo-hexos-4-ulosyl 249 β- Glucose β-Ribose 250 β-Glucose β-Lyxose 251 β-Glucose β-Allose 252 β-Glucose β-Altrose 253 β-Glucose β-Mannose 254 β-Glucose β-Gulose 255 β-Glucose β-Idose 256 β-Glucose β-Talose 257 β-Glucose β-Tagatose 258 β-Glucose β-Fmctose 259 β-Glucose c-6-Deoxy-xylo-hexos-4-ulosyl 260 β-Galactose β-Ribose 261 β-Galactose β-Lyxose 262 β -Galactose β-Allose 24 201024274 263 β-Galactose β-Altrose 264 β-Galactose β-Mannose 265 β-Galactose β-Gulose 266 β-Galactose β-Idose 267 β-Galactose β-Talose 268 β-Galactose β-Tagatose 269 β-Galactose β-Rmctose 270 α-Rhamnose β-Ribose 271 α-Rhamnose β-Lyxose 272 α-Rhamnose β-Allose 273 α-Rhamnose β-Altrose 274 α-Rhamnose β-Mannose 275 α-Rhamnose β-Gulose 276 α -Rhamnose β-Idose 277 α-Rhamnose β-Talose 278 α-Rhamnose β-Tagatose 279 α-Rhamnose β-Fmctose 280 c-6-Deoxy-xylo-hexos-4-ulosyl β-Ribose 281 c-6-Deoxy- Xylo-hexos-4-ulosyl β-Lyxose 282 c-6-Deoxy-xylo-hexos-4-ulosyl β- Allose 283 c-6-Deoxy-xylo-hexos-4-ulosyl β-Altrose 284 c-6-Deoxy-xylo-hexos-4-ulosyl β-Mannose 285 c-6-Deoxy-xylo-hexos-4-ulosyl β -Gulose 286 c-6-Deoxy-xylo-hexos-4-ulosyl β-Idose 25 201024274 287 c-6-Deoxy-xylo-liexos-4-ulosyl β-Talose 288 c-6-Deoxy-xylo-hexos-4 -ulosyl β-Tagatose 289 c-6-Deoxy-xylo-hexos-4-ulosyl β-Fractose According to the above concept, wherein Ri and R2 are the same or different functional groups, and are respectively selected from Cj-D-glucopyranosyl (CPD-glucopyranosyl), CfD-"C-β-D-galactopyranosyl, C-P_D-D than xylosyl (CP-D_xylopyranosyl), CPD-pyranosyl (CPD) -arabinopyranosyl), CaL- «CaL-arabinopyranosyl, CaL ° than C-a-L-rhamnopyranosyl and C-6-deoxy-xy-hex-4-glycosyl ( One of C^-deoxy-xylo-hexos-^ulosyl). In another aspect, the present invention provides a physiologically active composition comprising any one of the above three substances containing a structure of the formula 1, which has an effect of promoting the function of retinal pigment epithelial cells. According to the above concept, the composition further comprises a physiologically acceptable carrier, which is preferably a carrier. According to the above concept, the physiologically active composition is a pharmacologically active composition. According to the above concept, the physiologically active composition has pharmacological activity for a lesion selected from the group consisting of: retinal pigment epithelial cell degeneration, age-related macular degeneration, photoreceptor degeneration, diabetes, arteriosclerosis, vascular damage, retina Neurocyteopathy, axonal cells 26 201024274 Lesions, optic ganglion cell lesions, scleroderma lesions, uveitis, retinal angiogenesis, proliferative retinopathy, retinal inflammation, and at least one of Bruch's disease. For the sake of easy description, the present invention is fully understood by the following preferred embodiments and illustrations, and can be made by those skilled in the art. However, the embodiments of the present invention are not limited to the following implementations. In the example. [Embodiment] The extract of Dendrobium candidum and its process will be fully understood by the following examples, and can be completed by those skilled in the art, but the implementation of the present invention is not limited to the following implementations. In the example. (1) Preparation of the extract of Dendrobium candidum and separation of the active ingredient. Please refer to the first figure, which is a flow chart for the separation of the extract of Dendrobium candidum in the preferred embodiment of the present invention. The following methods can separate the three active ingredients in Dendrobium. 1.9 kg of Dendrobium was extracted three times with sterol (metJlan〇i) to obtain a Dendrobium methanol extract, and the Dendrobium methanol extract was concentrated and completely dried to form a DCM (Dendrobium crude extract) standard. The dried DCM standard was dissolved in 2 L of ethanol (Et〇Ac) and layered with 2 L of water to obtain an Et0Ac layer and a first aqueous layer. The first aqueous layer was extracted twice more with 2 L of EtOAc. All Et.sub.1 Ac. layer was collected and concentrated and completely bet to obtain the material. The ethanol extract of the money was layered three times with 4 L of hexane & 2 L of decyl alcohol to obtain a hexane layer and a methanol layer. After concentration and drying, the dried hexose layer and the methanol layer were named DCMPe/h standard (Pe/h) and 27 201024274 DCMPe/m standard (Pe/m), respectively. In addition, the first aqueous layer was adjusted to 2 liters by adding deionized water, and then subjected to partition extraction with 2 liters of buantol to obtain a butanol layer and a second aqueous layer, which were concentrated and concentrated after decompression. The dried butanol layer and the second aqueous layer were named DCMPb standard (Pb) and DCMPw standard (Pw), respectively. The PCMPb standard was subjected to molecular column chromatography (2.5 x 107 cm, mobile phase: methanol: water = 50:50) with LH20 • gel. After screening for activity, a standard named pcMPbL6,7 was obtained. Then, the adsorption column layer dialysis (1x30 cm) was carried out with Diaion SP-20 SS. When the mobile phase was isorpopanol: water = 20:80, a scouring product named DCMPbL6, 7D2 was obtained. When the mobile phase is isopropanol: water = 30:70, a rinse product designated DCMPbL6, 7D3 can be obtained. When the mobile phase is isopropyl alcohol (isorpopanol)·water = 40:60, a scouring product designated DCMPbL6, 7D4 can be obtained. Next, DCMPbL6, 7D2 was further subjected to HPLC reverse phase C18 column (10x300 mm). The mobile phase was methanol:water:acetic acid=35:65:1, and the name was collected as DCMPbL6, 7D2H2. Rushing the product. Based on the results of previous studies, DCMPbL6, 7D2H2 is known to have the effect of accelerating retinal pigment epithelial cell swallowing and increasing the degradation of highly glycated end products. The following steps are intended to further understand the efficacy of accelerating the retinal pigment epithelial cells. DCMPbL6, 7D2H2 is further subjected to HPLC reverse phase C18 column (10 χ 300 mm). When the mobile phase is decyl alcohol: water: acetic acid = 40:60:1, it can be obtained as DCMPbL6, 7D2H2H3. The rush 28 201024274 product. Next, DCMPbL6, 7D2H2H3 was further subjected to HPLC reverse phase C18 chromatography column (4.6 X 250 mm), and the mobile phase was decyl alcohol: water = 35:65, and a punch named DCMPbL6, 7D2H2H3H2 was obtained. Lift the product. DCMPbL6, 7D2H2H3 can also be extracted by HPLC reverse phase (C36) column (4.6x 250 mm) when the mobile phase is methanol: water = 40:60, and the dye named DCMPbL6, 7D2H2H3H3 can be obtained. Lift the product. Alternatively, DCMPbL6, 7D2H2H3 can also be subjected to ❹ HPLC reverse phase C18 chromatography column (4.6 X 250 mm) when the mobile phase is methanol: water = 45:55, and the name is DCMPbL6, 7D2H2H3H4; t extract product. Please refer to the second figure, which is the result of analysis of the Dendrobium extract DCMPbL6, 7D2H2H3 by LC-MS column chromatography. The analysis conditions in the second figure are: Mightysil RP-C18 column (4.6 x 250 mm), 5μιη; mobile phase at 0-80 minutes is methanol/water = 20/80 to 100% methanol gradient, 8th _ 1〇〇% is methanol, the injection volume is μΐ, the injection weight is 5pg; the flow rate is 〇25 ml/min, and the UV wavelength is 337 nm. Please refer to the third figure for the analysis of the Dendrobium extract DCMPbL6, 7D2H2H3 in this case by HPLC column chromatography. The analysis conditions in the second figure are: Mightysil RP-C18 column (4·6 X 250 mm), 5 μιη; mobile phase at the 〇_4〇 minute is sterol/water = 35/65, 40- At 45 minutes, it was a gradient of sterol/water = 35/65 to 1% sterol, 1 〇〇% methanol at 45-60 minutes; injection volume 25 〇 201024274 μΐ; injection weight 250 pg; flow rate ο 』ml/min at uv wavelengths of 280 nm, 312 nm and 337 nm. Wherein H2 represents DCMPbL6, 7D2H2H3H2, H3 represents DCMPbL6, 7D2H2H3H3 and H4 represents DCMPbL6, 7D2H2H3H4. • Refer to the fourth figure, which is the result of analysis of the Dendrobium extract DCMPbL6, 7D2H2H3H2 in this case by LC_MS column chromatography. The analysis conditions in the fourth graph are: Mightysil RP_ci8 column (4.6 x 250mm), 5 μπι; the mobile phase is the gradient of decyl alcohol/water = 20/8 〇 to 100% methanol at the first 〇8 minutes. 80-100 minutes is 100% sterol; injection volume is 100 μl; injection weight is 5; flow rate is 〇8 ml/min, at a UV wavelength of 22 〇 nm and the mass spectrometer (MS) is set to Αρα+ mode. Please refer to the fifth figure, which is the result of another analysis of the Dendrobium extract DCMPbL6, 7D2H2H3H2 in this case by LC-MS column chromatography. The analysis conditions in the fifth graph are: RP_C18 column (4.6 x 250 mm), 5 μηι; mobile phase at the _ • minute is the gradient of sterol/water = 20/80 to 1% sterol, 8 (M〇〇 minute is 100% sterol; injection volume is 10〇4; injection weight is 5 tons; flow rate is 0.8 ml/min, at UV wavelength 220 nm and mass spectrometer setting: APCI-mode. " Refer to the sixth figure, which is the result of analysis of the Dendrobium extract DCMPbL6, 7D2H2H3H3 in this case by LC-MS column chromatography. The analysis conditions in the sixth figure are: Mightysil column (4.6 x 250mm), 5 μιη; The phase is 201024274 sterol/water = 20/80 to 100% methanol gradient at the first 〇·8〇 minutes, 100% methanol at 80-100 minutes; injection volume 100 μΐ; injection weight 5 pg; flow rate 〇.8 Ml/min, under the condition of UV wavelength 220 nm and mass spectrometer set to APCI+ mode. Please refer to the seventh figure, which is the XJV spectrum of Dendrobium extract. DCMPbL6, 7D2H2H3H4 dissolved in sterol solution. As shown in the seventh figure, DCMPbL6, 7D2H2H3H4 has a first broadband (band I) at a wavelength (λ) of 334 nm and a wavelength of 275 n. m time © has a second broadband (band II), so it can be inferred that the compound belongs to a flavonoid compound. From the above results, it can be found that DCMPbL6, 7D2H2H3 has a molecular weight of about 534, and may be selected from celery glycoside 6 , 8-di-CaL-arabinopyranoside (apigenin 6,8-Di-CaL-arabinopyranoside), celery orthodontic 6-(Γ-β·Ι)-slightly 嗔xylyl-8-C-cx -L- 嗔 arabinose (apigenin 6-CpD-Xylopyranosyl-8-CaL- arabinopyranoside), celery with vinegar 6-CaL-0 ubiquitin arabinose base-8-C-P_D- a than xygenin (apigenin 6-CaL-

One of Arabinopyranosyl-8-C-p-D-xylopyranoside) and mixtures thereof. The structure of the celery glycoside 6-C-P-D-piperanose-8-C-a-L-pyranose is as follows: 31 201024274

Further, the results of infrared analysis of DCMPbL6, 7D2H2H3 (not shown) also show that the structural characteristics of DCMPbL6, 7D2H2H3 are consistent with the structural characteristics of the 6,8-diglucoside of the celery glycoside. In addition, some reagents (chlorinated, sodium acetate or a mixed solution of sodium acetate and boric acid) were added to DCMPbL6, 7D2H2H3H2, DCMPbL6, 7D2H2H3H3, DCMPbL6, 7D2H2H3H2, and DCMPbL6, 7D2H2H3H4, and the results were analyzed by UV. UV results show the structural characteristics of DCMPbL6, 7D2H2H3H2, DCMPbL6, 7D2H2H3H3, DCMPbL6, 7D2H2H3H2 and DCMPbL6, 7D2H2H3H4 and flavonoid compounds (hydroxyl group at C·5 and C·7 positions and hydrogen at C-3 position) The structural characteristics of the oxy) are consistent. After NMR analysis, hydrogen spectroscopy (H-NMR) and carbon spectroscopy (C-NMR) of DCMPbL6, 7D2H2H3, DCMPbL6, 7D2H2H3H2, DCMPbL6, 7D2H2H3H3 and 32 201024274 DCMPbL6, 7D2H2H3H4 showed corresponding structural features and celery. The structural features of the 6,8-di-C-glycoside of the saccharide are consistent. From the above results, it can be found that substances derived from DCMPbL6, 7D2H2, such as DCMPbL6, 7D2H2H3, DCMPbL6, 7D2H2H3H2, DCMPbL6, 7D2H2H3H3 and DCMPbL6, 7D2H2H3H4, may be 6,8-di-C-glycosides of celery glycoside or derived therefrom. And have the following structure. Reference r2

5 Ο

OH wherein l and R2 are the same or different functional groups and are respectively selected from one of α-arabose (α-Ara), β-arabinose (β-Ara) and β-xylose (β-Xyi). Since the natural C-glycosyl-branched C-glycosyl flavonoids include CpD-glucopyranosyl, CpD-» galactopyranosyl, C-β-Ε)- »比β-D-xylopyranosyl, CpD-e, arabinose (CpD-arabinopyranosyl) 'GaL- 0, arabinose (CaL-arabinopyranosyl), CaL °th rhamnosyl (CaL -rhamnopyranosyl) and C-6-deoxy-wood-hexa-4-glycosyl 33 201024274 (C-6-deoxy-xylo-hexos-4-ulosyl) 'and the above experiments have shown DCMPbL6, 7D2H2H3, DCMPbL6, 7D2H2H3H2, DCMPbL6 , 7D2H2H3H3 and DCMPbL6, 7D2H2H3H4 all have the main structure of the C-glycoside as shown in the previous paragraph, so when K and R2 are selected from the above-mentioned seven days of c-glycosyl flavonoids, 48 corresponding species will be obtained. Compound. Similarly, when R! and R2 are selected from non-natural C-glycosyl branches, 482 corresponding compounds will be obtained therefrom. From this, it can be seen that if the functional groups at the C-4, C-5 and C-7 positions are substituted by other functional groups, the number of corresponding derivatives will become enormous. The possible combinations of natural functional groups of 1^ and R2 are shown in Table 1. Table I, 艮 and 112 possible natural functional group combination compounds Ri r2 1* α-arabinose (a-Ara) a-Ara 2* β-xylose (β-Xyl) a-Ara 3* a-Ara β- Xyl 4 β-arabinose (β-Ara) β-Ara 5 a-Ara β-Ara 6 β-Ara a-Ara 7 β-Xyl β-Ara 8 β-Ara β-Xyl 9 c-6-deoxy- -6-Deoxy-xyl〇-hexos-4-ulosyl a-Ara 10 c-6-Deoxy-xyl〇-hexos-4-ulosyl β-Ara 11 c-6-Deoxy- Xyl〇-hexos-4-ulosyl β-Xyl 12 β-Xyl c-6-Deoxy-xylo-hexos-4-ulosyl 34 201024274 13 α-Ara c-6-Deoxy-xylo-hexos-4-ulosyl 14 β- Ara c-6-Deoxy-xylo-hexos-4-ulosyl 15 c-6-Deoxy-xylo-hexos-4-ulosyl c-6-Deoxy-xylo-hexos-4-ulosyl 16 α-Ara β-glucose (β -Glu) 17 α-Ara β-galactose (β-Gal) 18 β-Ara β-Glu 19 β-Ara β-Gal 20 β-Glu α-Ara 21 β-Gal α-Ara 22 β-Glu β- Ara 23 β-Gal β-Ara 24 β-Xyl β-Glu 25 β-Xyl β-Gal 26 β-Glu β-Xyl 27 β-Gal β-Xyl 28 c-6-Deoxy-xylo-hexos-4-ulosyl β-Glu 29 c-6-Deoxy-xylo-hexos-4-ulosyl β-Gal 30 β-Glu c-6-Deoxy-xylo-hexos-4-ulosyl 31 β-Gal c-6_Deoxy-xylo-hexos-4 - Ulosyl 32 a-Ara CX·rhamnose (α-Rha) 33 β-Ara α-Rha 34 a-Rha α-Ara 35 α-Rha ' β-Ara 36 β-Xyl α-Rha 37 a-Rha β- Xyl 35 201024274 38 c-6-Deoxy-xylo-hexos-4-ulosyl α-Rha 39 a-Rha c-6-Deoxy-xylo-hexos-4-ulosyl 40 β-Glu β-Glu 41 β-Glu β- Gal 42 β-Gal β-Glu 43 β-Gal β-Gal 44 β-Glu α-Rha 45 α-Rha β-Glu 46 β-Gal α-Rha 47 α-Rha β-Gal 48 α-Rha α-Rha * represents the main product. Possible non-natural functional group compositions of R4 & R2 are shown in Table 2. Table 2: ^ and! ^ Possible non-natural functional composition compounds shame (or r2) team (or shame) 1 β-ribose (β-Ribose) β-Ribose 2 β-Ribose a-Arabinose 3 β-Ribose β-Arabinose 4 β-Ribose β-Xylose 5 β-Ribose β_ribose (β-Lyxose) 6 β-Ribose β-allose 7 β-Ribose β-Altrose 36 β24

8 β-Ribose β-mannose (β-Mannose) 9 β-Ribose β-gulose (β-Gulose) 10 β-Ribose β-idulose (β-Idose) 11 β-Ribose β-Talose (β-Talose) 12 β-Ribose β-Tagatose 13 β-Ribose β-Fmctose 14 β-Ribose β-Glucose 15 β-Ribose β-Galactose 16 β-Ribose α -Rhamnose 17 β-Ribose c-6-Deoxy-xylo-hexos-4-ulosyl 18 α-Arabinose β-Ribose 19 α-Arabinose β-Lyxose 20 α-Arabinose β-Allose 21 α-Arabinose β-Altrose 22 α- Arabinose β-Mannose 23 α-Arabinose β-Gulose 24 α-Arabinose β-Idose 25 α-Arabinose β-Talose 26 α-Arabinose β-Tagatose 27 α-Arabinose β-Fmctose 28 β-Arabinose β-Ribose 37 201024274

29 β-Arabinose β-Lyxose 30 β-Arabinose β-Allose 31 β-Arabinose β-Altrose 32 β-Arabinose β-Mannose 33 β-Arabinose β-Gulose 34 β-Arabinose β-Idose 35 β-Arabinose β-Talose 36 β-Arabinose β-Tagatose 37 β-Arabinose β-Fructose 38 β-Xylose β-Ribose 39 β-Xylose β-Lyxose 40 β-Xylose β-Allose 41 β-Xylose β-Altrose 42 β-Xylose β-Mannose 43 β -Xylose β-Gulose 44 β-Xylose β-Idose 45 β-Xylose β-Talose 46 β-Xylose β-Tagatose 47 β-Xylose β-Fructose 48 β-Lyxose β-Ribose 49 β-Lyxose α-Arabinose 38 201024274

50 β-Lyxose β-Arabinose 51 β-Lyxose β-Xylose 52 β-Lyxose β-Lyxose 53 β-Lyxose β-Allose 54 β-Lyxose β-Altrose 55 β-Lyxose β-Mannose 56 β-Lyxose β-Gulose 57 β-Lyxose β-Dose 58 β-Lyxose β-Talose 59 β-Lyxose β-Tagatose 60 β-Lyxose β-Fructose 61 β-Lyxose β-Glucose 62 β-Lyxose β-Galactose 63 β-Lyxose α-Rhamnose 64 β -Lyxose c-6-Deoxy-xylo-hexos-4-ulosyl 65 β-Allose β-Ribose 66 β-Allose α-Arabinose 67 β-Allose β-Arabinose 68 β-Allose β-Xylose 69 β-Allose β-Lyxose 70 β-Allose β-Allose 39 201024274

71 β-Allose β-Altrose 72 β-Allose β-Marmose 73 β-Allose β-Gulose 74 β-Allose β-Idose 75 β-Allose β-Talose 76 β-Allose β-Tagatose 77 β-Allose β-Fructose 78 β-Allose β-Glucose 79 β-Allose β-Galactose 80 β-Allose α-Rhamnose 81 β-Allose c-6-Deoxy-xylo-hexos-4-ulosyl 82 β-Altrose β-Ribose 83 β-Altrose α- Arabinose 84 β-Altrose β-Arabinose 85 β-Altrose β-Xylose 86 β-Altrose β-Lyxose 87 β-Altrose β-Allose 88 β-Altrose β-Altrose 89 β-Altrose β-Mannose 90 β-Altrose β-Gulose 91 β-Altrose β-Idose 201024274

92 β-Altrose β-Talose 93 β-Altrose β-Tagatose 94 β-Altrose β-Fructose 95 β-Altrose β-Glucose 96 β-Altrose β-Galactose 97 β-Altrose α-Rhamnose 98 β-Altrose c-6- Deoxy-xylo-hexos-4-ulosyl 99 β-Mannose β-Ribose 100 β-Mannose α-Arabinose 101 β-Mannose β-Arabinose 102 β-Mannose β-Xylose 103 β-Mannose β-Lyxose 104 β-Mannose β- Allose 105 β-Mannose β-Altrose 106 β-Mannose β-Mannose 107 β-Mannose β-Gulose 108 β-Mannose β-Idose 109 β-Mannose β-Talose 110 β-Mannose β-Tagatose 111 β-Mannose β-Fructose 112 β-Mannose β-Glucose 41 201024274 113 β-Mannose β-Galactose 114 β-Mannose α-Rhamnose 115 β-Mannose c-6-Deoxy-xylo-hexos-4-ulosyl 116 β-Gulose β-Ribose 117 β- Gulose α-Arabinose 118 β-Gulose β-Arabinose 119 β-Gulose β-Xylose 120 β-Gulose β-Lyxose 121 β-Gulose β-Allose 122 β-Gulose β-Altrose 123 β-Gulose β-Mannose 124 β-Gulose β-Gulose 125 β-Gulose β-Idose 126 β-Gulose β-Talose 127 β-Gulose β-Tagato Se 128 β-Gulose β-Fructose 129 β-Gulose β-Glucose 130 β-Gulose β-Galactose 131 β-Gulose α-Rhamnose 132 β-Gulose c-6-Deoxy-xylo-hexos-4-ulosyl 133 β-Idose β-Ribose 42 201024274 134 β-Idose α-Arabinose 135 β-Idose β-Arabinose 136 β-Idose β-Xylose 137 β-Idose β-Lyxose 138 β-Idose β-Allose 139 β-Idose β-Altrose 140 β- Idose β-Mannose 141 β-Idose β-Gulose 142 β-Idose β-Idose 143 β-Idose β-Talose 144 β-Idose β-Tagatose 145 β-Idose β-Fmctose 146 β-Idose β-Glucose 147 β-Idose β-Galactose 148 β-Idose α-Rhamnose 149 β-Idose c-6-Deoxy-xylo-hexos-4-ulosyl 150 β-Talose β-Ribose 151 β-Talose α-Arabinose 152 β-Talose β-Arabinose 153 β -Talose β-Xylose 154 β-Talose β-Lyxose 43 201024274

155 β-Talose β-Allose 156 β-Talose β-Altrose 157 β-Talose β-Mannose 158 β-Talose β-Gulose 159 β-Talose β-Idose 160 β-Talose β-Talose 161 β-Talose β-Tagatose 162 β-Talose β-Fmctose 163 β-Talose β-Glucose 164 β-Talose β-Galactose 165 β-Talose α-Rhamnose 166 β-Talose c-6-Deoxy-xylo-hexos-4-ulosyl 167 β-Tagatose β- Ribose 168 β-Tagatose α-Arabinose 169 β-Tagatose β-Arabinose 170 β-Tagatose β-Xylose 171 β-Tagatose β-Lyxose 172 β-Tagatose β-Allose 173 β-Tagatose β-Altrose 174 β-Tagatose β-Mannose 175 β-Tagatose β-Gulose 44 201024274

176 β-Tagatose β-Idose 177 β-Tagatose β-Talose 178 β-Tagatose β-Tagatose 179 β-Tagatose β-Fructose 180 β-Tagatose β-Glucose 181 β-Tagatose β-Galactose 182 β-Tagatose α-Rhamnose 183 β-Tagatose c-6-Deoxy-xylo-hexos-4-ulosyl 184 β-Fmctose β-Ribose 185 β-Fructose α-Arabinose 186 β-Fmctose β-Arabinose 187 β-Fmctose β-Xylose 188 β-Fructose β- Lyxose 189 β-Fmctose β-Allose 190 β-Fructose β-Altrose 191 β-Fmctose β-Mannose 192 β-Fmctose β-Gulose 193 β-Fmctose β-Idose 194 β-Fructose β-Talose 195 β-Fmctose β-Tagatose 196 β-Fmctose β-Fructose 45 201024274

197 β-Fmctose β-Glucose 198 β-Fructose β-Galactose 199 β-Fmctose α-Rhamnose 200 β-Fructose c-6-Deoxy-xylo-hexos-4-ulosyl 201 β-Glucose β-Ribose 202 β-Glucose β -Lyxose 203 β-Glucose β-Allose 204 β-Glucose β-Altrose 205 β-Glucose β-Mannose 206 β-Glucose β-Gulose 207 β-Glucose β-Idose 208 β-Glucose β-Talose 209 β-Glucose β- Tagatose 210 β-Glucose β-Fructose 211 β-Glucose c-6-Deoxy-xylo-hexos-4-ulosyl 212 β-Galactose β-Ribose 213 β-Galactose β-Lyxose 214 β-Galactose β-Allose 215 β-Galactose β-Altrose 216 β-Galactose β-Mannose 217 β-Galactose β-Gulose 46 201024274

218 β-Galactose β-Idose 219 β-Galactose β-Talose 220 β-Galactose β-Tagatose 221 β-Galactose β-Fructose 222 α-Rhamnose β-Ribose 223 α-Rhanmose β-Lyxose 224 α-Rhamnose β-Allose 225 α-Rhamnose β-Altrose 226 α-Rhamnose β-Mannose 227 α-Rhamnose β-Gulose 228 α-Rhamnose β-Idose 229 α-Rhamnose β-Talose 230 α-Rhamnose β-Tagatose 231 α-Rhamnose β-Fructose 232 c -6-Deoxy-xylo-hexos-4-ulosyl β-Ribose 233 c-6-Deoxy-xylo-hexos-4-ulosyl β-Lyxose 234 c-6-Deoxy-xylo-hexos-4-ulosyl β-Allose 235 c-6-Deoxy-xylo-hexos-4-ulosyl β-Altrose 236 c-6-Deoxy-xylo-hexos-4-ulosyl β-Mannose 237 c-6-Deoxy-xylo-hexos-4-ulosyl β-Gulose 238 c-6-Deoxy-xylo-hexos-4-ulosyl β-Idose 47 201024274 239 c-6-Deoxy-xylo-hexos-4-ulosyl β-Talose 240 c-6-Deoxy-xylo-hexos-4-ulosyl β-Tagatose 241 c-6-Deoxy-xylo-hexos-4-ulosyl β-Fmctose Please refer to the eighth figure, which is a bar graph of the effect of the Dendrobium extract DCMPbL6, 7D2H2H3 on the overall swallowing effect of RPE cells. As shown in Figure 8, different concentrations of DCMPbL6, 7D2H2H3 (0.1, 1, 10, and 100 pg/ml) can accelerate retinal pigment epithelial cells.

The swallowing effect of & the relevant experimental content is briefly described below. 1 X 1〇4 RPE cells were placed in a 96-well plate and cultured in DMEM medium containing 5% fetal calf serum for 48 hours, and then the culture medium was changed to a DMEM medium containing 2% fetal calf serum. Then, different concentrations of DCMPbL6, 7D2H2H3 were added, and after 24 hours, 50 μL of 2 X 107/ml calibrated FITC-fluorescent ROS cells (FITC-ROS) were added to each culture medium, and after mixing for 4 hours, mix 2 The phosphate buffer of % sucrose washed off FITC-ROS that did not adhere to the cell surface. The emission intensity is detected by the 1420 Multilable counter (PE) measurement system at an emission wavelength of 485 485 nm and a detection wavelength of 530 nm, and the detected fluorescence intensity represents the result of overall swallowing. If a fluro Quench dye is added, the measured fluorescence intensity represents the result of intracellular swallowing. Compared with the swallowing effect of RPE cells treated with 2% fetal bovine serum, * represents a p value of less than 0.05, ** represents a P value less than 〇. 〇 2, *** represents a P value of less than 0. (^ and **** The representative P value is less than 0.001. In addition, the ninth figure is a bar graph of the effect of the Dendrobium extract DCMPbL6, 7D2H2H3 on the intracellular swallowing effect of RPE cells. 48 201024274 Please refer to the tenth figure, which is the Dendrobium extract DCMPbL6. , a bar graph of the effect of 7D2H2H3H2 on the overall swallowing effect of RPE cells. As shown in the tenth figure, different concentrations of DCMPbL6, 7D2H2H3H2 (0.1, 1, 10 and 1〇〇gg/mi) can accelerate the retinal pigment epithelial cells. Swallowing effect, a brief description of the relevant experimental contents - as follows: 1 X l〇4RPE cells were placed in a 96-well plate, cultured in DMEM medium containing 5 〇 / 〇 fetal bovine serum for 48 hours, and the culture medium was changed to contain 2% fetal bovine serum in DMEM medium. Then, different concentrations of DCMPbL6, 7D2H2H3H2 were added, and 24 hours later, 50 μM of 2 X 10 FITC-ROS/ml was added to each culture medium, and after 4 hours of culture, FITC-R not attached to the cell surface with phosphate buffer mixed with 2% sucrose The OS is washed off. The emission wavelength is 485 nm, and the wavelength is 530 nm. The fluorescence intensity is measured by the 1420 Multilable counter (PE) measurement system, and the detected fluorescence intensity represents the result of the overall swallowing effect. For Quench dyes, the detected fluorescence intensity represents the result of intracellular swallowing. Compared with the swallowing effect of RPE cells treated with 2% fetal bovine serum, * represents P value less than 0.05, ** represents P The value is less than 0.02, *** represents P value is less than 〇.〇1 and *Material* represents P value less than 0.001. In addition, the eleventh figure is the effect of the Dendrobium extract DCMPbL6, 7D2H2H3H2 on the intracellular swallowing effect of RPE cells. Column chart. For the month of δ, see the eleventh figure, which is the column diagram of the effect of the Dendrobium extract DCMPbL6, 7D2H2H3H3 on the overall swallowing effect of RPE cells. As shown in Figure 12, different concentrations of 49 201024274 DCMPbL6, 7D2H2H3H3 (0.1, Bu 10 and 100 pg/ml) can accelerate the phagocytosis of retinal pigment epithelial cells. The relevant experimental contents are briefly described as follows: 1 X 104RPE cells were placed in a 96-well plate containing $ 〇 / abortion Bovine serum in DMEM culture medium After 48 hours of incubation, the culture medium was changed to DMEM medium containing 2% fetal bovine serum. Then, different concentrations of DCMPbL6, 7D2H2H3H3 were added, and after 24 hours, 50 μL of 2 X 107 FITC-ROS/ml was added to each culture. In the cell, after 4 hours of culture, the FITC-ROS not attached to the cell surface was washed away by mixing 2% of the food and sugar in the citrate buffer. The emission wavelength is 485 nm and the detection wavelength is 530 nm. The fluorescence intensity is detected by the 1420 Multilable counter (PE) measurement system, and the detected fluorescence intensity represents the result of the overall swallowing effect. If a fluro Quench dye is added, the detected fluorescence intensity represents the result of intracellular swallowing. Compared with the swallowing effect of RPE cells treated with 2% fetal bovine serum, * represents p value less than 〇〇5, ** represents P value less than 0.02, *** represents P value less than 〇.〇1 and **** represents The P value is less than 0.001. In addition, the thirteenth figure is a bar graph of the effect of the Dendrobium extract DCMPbL6, 7D2H2H3H3 on the intracellular swallowing effect of RPE cells. Please refer to the fourteenth figure, which is a bar graph of the effect of Dendrobium extract DCMPbL6, 7D2H2H3H4 on the overall swallowing effect of RPE cells. As shown in Figure 14, different concentrations of DCMPbL6, 7D2H2H3H4 (0.1, 1, 1〇 and 1〇〇 gg/mi) can accelerate the swallowing effect of retinal pigment epithelial cells. The relevant experimental contents are summarized as follows. 1 X 104 RPE cells were placed in a 96-well plate and cultured in DMEM medium containing 5% 50 201024274 fetal bovine serum for 48 hours, and then the culture medium was changed to DMEM medium containing 2% fetal calf serum. Then, different concentrations of 〇〇ΜΡ1^6,7ϋ2Η2Η3ΙΙ4 were added separately. After 24 hours, 50 μL of 2 X 107 FITC-ROS/ml was added to each culture medium, and after 4 hours of cultivation, 'mix 2% sucrose phosphate. The buffer washed off FITC-ROS that did not adhere to the cell surface. The emission wavelength is 485 nm and the detection wavelength is 530 nm. The fluorescence intensity is detected by the 1420 Multilable counter (PE) measurement system, and the detected fluorescence intensity represents the result of the overall swallowing effect. If fhiro Quench is added, the detected glory intensity represents the result of intracellular swallowing. Compared with the swallowing effect of RPE cells treated with 2% fetal bovine serum, * represents p value less than 〇〇5, ** represents P value less than 0.02, *** represents P value less than 〇.〇1 and **** represents The P value is less than 0.001. In addition, the fifteenth figure is a bar graph of the effect of the Dendrobium extract DCMPbL6, 7D2H2H3H4 on the intracellular swallowing effect of RPE cells. According to the second to seventh figures, DCMPbL6, 7D2H2H3, DCMPbL6, 7D2H2H3H2 - DCMPbL6, 7D2H2H3H3 and DCMPbL6, 7D2H2H3H4, may be 6,8-di-(:-glycoside or a derivative thereof of celery glycoside, and have the following structure.

5 OH 0 51 201024274 Which 仏 and! ^ is the same or different functional groups and is selected from one of the combinations in Tables 1 and 2, respectively. According to the eighth to fifteenth figures, DCMPbL6, 7D2H2H3, DCMPbL6, 7D2H2H3H2 'DCMPbL6, 7D2H2H3H3 and DCMPbL6, 7D2H2H3H4 can accelerate the engulfing effect of retinal pigment epithelial cells. As described above, it should be understood that the substance having the above structure accelerates the swallowing action of the retinal pigment epithelial cells. A study based on previous literature 'comprising a composition that accelerates the swallowing action of retinal pigment epithelial cells, is effective for the following lesions: retinal pigment epithelial cell degeneration, age-related macular degeneration, photoreceptor degeneration, diabetes (eg: vascular disease), arteriosclerosis, vascular damage, retinal neurocytosis, axonal cytopathic lesions, optic ganglion cell lesions, visceral lesions, uveitis, retinal angiogenesis, proliferative retinopathy, retinal inflammation And at least one of Bruch's membrane lesions. In addition, because the practical application of this case is very large, it is not limited to the engulfing effect of retinal pigment epithelial cells, but on the enhancement of physiological functions of retinal epithelial cells and the recovery of impaired function, and it is related to The first case has a clear chemical structure, so the present invention has significant novelty and progress, and even if the relevant implementation of the present case can be modified by the general knowledge in this field, it can be modified as appropriate. The person who wants to protect. 52 201024274 [Simplified description of the drawings] First: Flow chart of separation of Dendrobium extract in the preferred embodiment of the present invention; 'Second picture: DHPbL6, 7D2H2H3 extract of Dendrobium L. in this case by LC-MS column chromatography The results obtained by the analysis; The third picture: the results of the analysis of the Dendrobium extract DCMPbL6, 7D2H2H3 in this case by HPLC column chromatography; ❹ The fourth picture: the Dendrobium extract DCMPbL6, 7D2H2H3H2 in this case is LC-MS column The results obtained by chromatography were analyzed. The fifth figure: the result of another analysis of the Dendrobi extract DCMPbL6'7D2H2H3H2 in this case by LC-MS column chromatography; Figure 6: Dendrobium extract DCMPbL6, 7D2H2H3H3 The results obtained by LC-MS column chromatography were analyzed. The seventh figure: the uv spectrum of the Dendrobi extract DCMPbL6, 7D2H2H3H4 dissolved in the sterol solution of this case; The eighth figure: the Dendrobium extract of this case Bar graph of the effect of DCMPbL6, 7D2H2H3 on the overall swallowing effect of RPE cells; Figure 9: Bar graph of the effect of DCMPbL6, 7D2H2H3 on the intracellular swallowing effect of RPE cells in this case; 53 201024 274 The tenth figure: the column diagram of the effect of DCMPbL6, 7D2H2H3H2 on the whole swallowing effect of RPE cells in this case; Figure 11: The effect of DCMPbL6, 7D2H2H3H2 on the intracellular swallowing of RPE cells in this case Column chart; Twelfth chart: the column diagram of the influence of DCMPbL6, 7D2H2H3H3 on the whole swallowing effect of RPE cells in this case; Figure 13: DHPbL6, 7D2H2H3H3 vs. RPE cells of Dendrobium extract A bar graph of the effects of intracellular swallowing; Figure 14: a bar graph of the effects of DCMPbL6, 7D2H2H3H4 on the overall swallowing effect of RPE cells in this case; and fifteenth figure: Dendrobium extract of this case A bar graph of the effect of DCMPbL6, 7D2H2H3H4 on intracellular swallowing of RPE cells. [Main component symbol description] None 54

Claims (1)

201024274 X. Patent application scope: 1. A composition comprising: a substance having the structure of formula one, OH ζ One of the same and different functional groups in the center and R2. 2. The composition of claim 1, wherein the composition has a physiological activity. 3. The composition of claim 1, wherein the composition comprises an effect of accelerating the swallowing action of the retinal pigment epithelial cells. 4. The composition of claim 1 wherein the substance is selected from the group consisting of a 6,f-tolerant 6,8-di-CaL-11-pyranose (apjgenin 6,8-Di-CaL-arabinopyranoside) ) ' One celery with ® I body 6_C-PD-pyrose glucosyl-SCaL-Bth yoghurt (apigenin 6-Cp, D-Xyl〇pyranosyl-8-CaL-arabinopyranoside), a celery glycoside 6- One of C-cx-L-pyraninosyl-8-(:-β-0-glucopyranose (apigenin 6-CaL-Arabinopyranosyl-8-CpD-xylopyranoside) and a mixture thereof. a substance having the structure of Formula 1, 55 5 ' 8 201024274 R2 Formula 0 0 OH wherein Ri and R 2 are respectively selected from α-arabinose (a-Ara), p-arabinose (β-Ara), and β-xylose ( 6. One of β-Xyl) 6. The substance of claim 5, which has an effect of promoting the activity of retinal pigment cells. 7. a substance having the structure of formula 1, R2 8 HO 3 Formula 1 OH 0 wherein 1^ and 112 are respectively selected from the following composition: R! (or R2) r2« Ri) 1 α-arabinose (a-Ara) α-Ara 2 P-xylose (β -Xyl) α-Ara 3 α-Ara β-Xyl 4 β-arabinose (β-Ara) β-Ara 5 a-Ara β-Ara 6 β-Ara α-Ara 7 β-Xyl β-Ara 56 201024274 8 β-Ara β-Xyl 9 c-6-degreas-wood-hex-4-glycosyl fc-6-Deoxy-xylo-hexos-4-ulosyl) α-Ara 10 c-6-Deoxy-xylo-hexos- 4-ulosyl β-Ara 11 c-6-Deoxy-xylo-hexos-4-ulosyl β-Xyl 12 β-Xyl c-6-Deoxy-xylo-hexos-4-ulosyl 13 α-Ara c-6-Deoxy- Xylo-hexos-4-ulosyl 14 β-Ara c-6-Deoxy-xylo-hexos-4-ulosyl 15 c-6-Deoxy-xylo-hexos-4-ulosyl c-6-Deoxy-xylo-hexos-4- Ulosyl 16 α-Ara β-glucose (β-Glu) 17 α-Ara P-galactose (P-Gal) 18 β-Ara β-Glu 19 β-Ara β-Gal 20 β-Glu α-Ara 21 β- Gal α-Ara 22 β-Glu β-Ara 23 β-Gal β-Ara 24 β-Xyl β-Glu 25 β-Xyl β-Gal 26 β-Glu β-Xyl 27 β-Gal β-Xyl 28 c-6 -Deoxy-xylo-hexos-4-ulosyl β-Glu 29 c-6-Deoxy-xylo-hexos-4-ulosyl β-Gal 30 β-Glu c-6-Deoxy-xylo-liexos-4-iilosyl 57 2010 24274 31 β-Gal c-6-Deoxy-xylo-hexos-4-ulosyl 32 α-Ara (X-rhamnose (α-Rha) 33 β-Ara α-Rha 34 α-Rha α-Ara 35 α- Rha β-Ara 36 β-Xyl α-Rha 37 α-Rha β-Xyl 38 c-6-Deoxy-xylo-hexos-4-ulosyl α-Rha 39 α-Rha c-6-Deoxy-xylo-hexos-4 -ulosyl 40 β-Glu β-Glu 41 β-Glu β-Gal 42 β-Gal β-Glu 43 β-Gal β-Gal 44 β-Glu α-Rha 45 α-Rha β-Glu 46 β-Gal α- Rha 47 α-Rha β-Gal 48 α-Rha α-Rha 49 β-Ribose β-Ribose 50 β-Ribose α-Arabinose 51 β-Ribose β-Arabinose 52 β-Ribose β-Xylose 53 β -Ribose β-Lyxose 54 β-Ribose β-Alose (β-Allose) 58 201024274 55 β-Ribose β-Altrose 56 β-Ribose β-Mannose 57 β-Ribose β-Gulose 58 β-Ribose β-idose (β-Idose) 59 β-Ribose β-Talose 60 β-Ribose β-Tagatose 61 β-Ribose β-Frucose (β-Fmctose) 62 β-Ribose β -Glucose 63 β-Ribose β-Galactose 64 β-Ribose α-Rhamnose 65 β-Ribose c-6-Deoxy-xylo-hexos-4-ulosyl 66 α-Arabinose β-Ribose 67 α-Arabinose β-Lyxose 68 α- Arabinose β-Allose 69 α-Arabinose β-Altrose 70 α-Arabinose β-Mannose 71 α-Arabinose β-Gulose 72 α-Arabinose β-Idose 73 α-Arabinose β-Talose 74 α-Arabinose β-Tagatose 75 α-Arabinose β-Fructose 76 β-Arabinose β-Ribose 77 β-Arabinose β-Lyxose 78 β-Arabinose β-Allose 59 201024274 79 β-Arabinose β-Altrose 80 β-Arabinose β-Mannose 81 β-Arabinose β-Gulose 82 β-Arabinose β-Idose 83 β-Arabinose β-Talose 84 β-Arabinose β-Tagatose 85 β-Arabinose β-Fructose 86 β-Xylose β-Ribose 87 β-Xylose β-Lyxose 88 β-Xylose β-Allose 89 β-Xylose β-Altrose 90 β-Xylose β-Mannose 91 β-Xylose β-Gulose 92 β-Xylose β-Idose 93 β -Xylose β-Talose 94 β-Xylose β-Tagatose 95 β-Xylose β-Fructose 96 β-Lyxose β-Ribose 97 β-Lyxose α-Arabinose 98 β-Lyxose β-Arabinose 99 β-Lyxose β-Xylose 100 β- Lyxose β-Lyxose 101 β-Lyxose β-Allose 102 β-Lyxose β-Altrose 201024274 103 β-Lyxose β-Mannose 104 β-Lyxose β-Gulose 105 β-Lyxose β-Idose 106 β-Lyxose β-Talose 107 β- Lyxose β-Tagatose 108 β-Lyxose β-Fructose 109 β-Lyxose β-Glucose 110 β-Lyxose β-Galactose 111 β-Lyxose α-Rhamnose 112 β-Lyxose c-6-Deoxy-xylo-hexos-4-ulosyl 113 β-Allose β-Ribose 114 β-Allose α-Arabinose 115 β-Allose β-Arabinose 116 β-Allo Se β-Xylose 117 β-Allose β-Lyxose 118 β-Allose β-Allose 119 β-Allose β-Altrose 120 β-Allose β-Mannose 121 β-Allose β-Gulose 122 β-Allose β-Idose 123 β-Allose β-Talose 124 β-Allose β-Tagatose 125 β-Allose β-Fructose 126 β-Allose β-Glucose 61 201024274 127 β-Allose β-Galactose 128 β-Allose α-Rhamnose 129 β-Allose c-6-Deoxy- Xylo-hexos-4-ulosyl 130 β-Altrose β-Ribose 131 β-Altrose α-Arabinose 132 β-Altrose β-Arabinose 133 β-Altrose β-Xylose 134 β-Altrose β-Lyxose 135 β-Altrose β-Allose 136 β-Altrose β-Altrose 137 β-Altrose β-Mannose 138 β-Altrose β-Gulose 139 β-Altrose β-Idose 140 β-Altrose β-Talose 141 β-Altrose β-Tagatose 142 β-Altrose β-Fructose 143 β -Altrose β-Glucose 144 β-Altrose β-Galactose 145 β-Altrose α-Rhamnose 146 β-Altrose c-6-Deoxy-xylo-hexos-4-ulosyl 147 β-Mannose β-Ribose 148 β-Mannose α-Arabinose 149 β-Mannose β-Arabinose 150 β-Mannose β-Xylose 62 201024274 151 β-Mannose β-Lyxose 152 β-Mannose β-Allose 153 β-Mannose β-Altrose 154 β-Mannose β-Mannose 155 β-Mannose β-Gulose 156 β-Mannose β-Idose 157 β-Mannose β-Talose 158 β-Mannose β-Tagatose 159 β-Mannose β-Fmctose 160 β-Mannose β-Glucose 161 β-Mannose β-Galactose 162 β-Mannose α-Rhamnose 163 β-Mannose c-6-Deoxy-xylo-hexos-4- Ulosyl 164 β-Gulose β-Ribose 165 β-Gulose α-Arabinose 166 β-Gulose β-Arabinose 167 β-Gulose β-Xylose 168 β-Gulose β-Lyxose 169 β-Gulose β-Allose 170 β-Gulose β-Altrose 171 β-Gulose β-Mannose 172 β-Gulose β-Gulose 173 β-Gulose β-Idose 174 β-Gulose β-Talose 63 201024274 175 β-Gulose β-Tagatose 176 β-Gulose β-Fructose 177 β-Gulose β- Glucose 178 β-Gulose β-Galactose 179 β-Gulose α-Rhamnose 180 β-Gulose c-6-Deoxy-xylo-hexos-4-ulosyl 181 β-Idose β-Ribose 182 β-Idose α-Arabinose 183 β-Idose β-Arabinose 184 β-Idose β-Xylose 185 β-Idose β-Lyxose 186 β-Idose β-Allose 187 -Idose β-Altrose 188 β-Idose β-Mannose 189 β-Idose β-Gulose 190 β-Idose β-Idose 191 β-Idose β-Talose 192 β-Idose β-Tagatose 193 β-Idose β-Fructose 194 β- Idose β-Glucose 195 β-Idose β-Galactose 196 β-Idose α-Rhamnose 197 β-Idose c-6-Deoxy-xylo-hexos-4-ulosyl 198 β-Talose β-Ribose 64 201024274 199 β-Talose α- Arabinose 200 β-Talose β-Arabinose 201 β-Talose β-Xylose 202 β-Talose β-Lyxose 203 β-Talose β-Allose 204 β-Talose β-Altrose 205 β-Talose β-Mannose 206 β-Talose β-Gulose 207 β-Talose β-Idose 208 β-Talose β-Talose 209 β-Talose β-Tagatose 210 β-Talose β-Fructose 211 β-Talose β-Glucose 212 β-Talose β-Galactose 213 β-Talose α-Rhamnose 214 β-Talose c-6-Deoxy-xylo-hexos-4-ulosyl 215 β-Tagatose β-Ribose 216 β-Tagatose α-Arabinose 217 β-Tagatose β-Arabinose 218 β-Tagatose β-Xylose 219 β-Tagatose β- Lyxose 220 β-Tagatose β-Allose 221 β-Tagatose β-Altrose 222 β-Tagatose β-Mannose 65 20102 4274 223 β-Tagatose β-Gulose 224 β-Tagatose β-Idose 225 β-Tagatose β-Talose 226 β-Tagatose β-Tagatose 227 β-Tagatose β-Fructose 228 β-Tagatose β-Glucose 229 β-Tagatose β-Galactose 230 β-Tagatose α-Rhamnose 231 β-Tagatose c-6-Deoxy-xylo-hexos-4-ulosyl 232 β-Fructose β-Ribose 233 β-Fructose α-Arabinose 234 β-Fructose β-Arabinose 235 β-Fmctose β -Xylose 236 β-Fructose β-Lyxose 237 β-Fmctose β-Allose 238 β-Fructose β-Altrose 239 β-Fructose β-Mannose 240 β-Fructose β-Gulose 241 β-Fructose β-Idose 242 β-Fructose β- Talose 243 β-Fructose β-Tagatose 244 β-Fructose β-Fructose 245 β-Fmctose β-Glucose 246 β-Fructose β-Galactose 66 201024274 247 β-Fructose α-Rhamnose 248 β-Fructose c-6-Deoxy-xylo- Hexos-4-ulosyl 249 β-Glucose β-Ribose 250 β-Glucose β-Lyxose 251 β-Glucose β-Allose 252 β-Glucose β-Altrose 253 β-Glucose β-Mannose 254 β-Glucose β-Gulose 255 β- Glucose β-Idose 256 β-Glucose β-Talose 257 -Glucose β-Tagatose 258 β-Glucose β-Fmctose 259 β-Glucose c-6-Deoxy-xylo-hexos-4-ulosyl 260 β-Galactose β-Ribose 261 β-Galactose β-Lyxose 262 β-Galactose β-Allose 263 β-Galactose β-Altrose 264 β-Galactose β-Mannose 265 β-Galactose β-Gulose 266 β-Galactose β-Idose 267 β-Galactose β-Talose 268 β-Galactose β-Tagatose 269 β-Galactose β-Fmctose 270 α-Rhamnose β-Ribose 67 201024274 271 a-Rhamnose β-Lyxose 272 a-Rhamnose β-Allose 273 α-Rhamnose β-Altrose 274 α-Rhamnose β-Mannose 275 α-Rhamnose β-Gulose 276 α-Rhamnose β-Idose 277 α-Rhamnose β-Talose 278 α-Rhamnose β-Tagatose 279 α-Rhamnose β-Fmctose 280 c-6-Deoxy-xylo-hexos-4-ulosyl β-Ribose 281 c-6-Deoxy-xylo-hexos-4 -ulosyl β-Lyxose 282 c-6-Deoxy-xylo-hexos-4-ulosyl β-Allose 283 c-6-Deoxy-xylo-hexos-4-ulosyl β-Altrose 284 c-6-Deoxy-xylo-hexos- 4-ulosyl β-Mannose 285 c-6-Deoxy-xylo-hexos-4-ulosyl β-Gulose 286 c-6-Deoxy-xylo-hexos-4-ulosyl β-Idose 287 c-6-Deoxy-xyl O-hexos-4-ulosyl β-Talose 288 c-6-Deoxy-xylo-hexos-4-ulosyl β-Tagatose 289 c-6-Deoxy-xylo-hexos-4-ulosyl β-Fmctose 8. The substance of claim 7, wherein 1 and 2 are the same or different functional groups, and are respectively selected from CPD-glucopyranosyl, CpD-β galactosyl (CPD-galactopyranosyl), CpD- ° ratio °Cylenose (CpD-xylopyranosyl) 'C-P_D- ° than arabinose 68 201024274 (Cp-D_arabinopyranosyl), CaL- ° than arabinose (CaL- Arabinopyranosyl) 'CaL 〇 味 鼠 鼠 鼠 鼠 Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca Ca . A physiologically active composition comprising the substance according to any one of claims 1, 5 or 7, which has an effect of promoting the function of retinal pigment epithelial cells. 10. The composition of claim 9 of the patent application, further comprising a physiologically acceptable carrier, such as the composition of the first aspect of the patent application, wherein the physiological activity The composition is a pharmacologically active composition. 12. The composition of claim 10, wherein the physiologically acceptable carrier is a pharmacological carrier. 13. The composition of claim n, wherein the physiologically active composition has pharmacological activity for a lesion selected from the group consisting of: retinal pigment epithelial cell degeneration, age-related macular degeneration, photoreceptor degeneration, diabetes Arteriosclerosis, vascular damage, retinal neurocycosis, axonal cytopathic lesions, optic ganglion cell lesions, scleroderma lesions, uveitis, retinal angiogenesis, proliferative retinopathy, retinal inflammation, and Bruch's membrane At least one of the lesions. 69