200928024 九、發明說明： 【發明所屬之技術領域】 且特別是有 本發明是有關於一種纖維與其製作方法 關於一種抗菌纖維與製作方法。200928024 IX. INSTRUCTIONS: [Technical field to which the invention pertains] In particular, the present invention relates to a fiber and a method of manufacturing the same, relating to an antibacterial fiber and a method of fabricating the same.
紡織品在曰常生活中隨處可見，其中又以 為常I伴隨著科技的進步，使转除了希望穿2 = 良好的保暖功能外，亦希望織物本身具有 = 如抗菌之效果。 <功能 除了使用者希望織物具有前述附加之功能外’ 商本身亦希望藉由製造具前述抗菌功效之織物，提高織物 之附加價值與應用領域。為達成上述之目I對織物纺織 用之纖維的改良為目前生產商努力的方向之一。 【發明内容】 本發明實施例提出一種抗菌纖維的製作方法及其所製 作之抗菌纖維。 依照本發明一實施例’提出一種抗菌纖維的製作方 法°首先’提供重量平均分子量約為10,000〜50 000的幾丁 聚糖。之後’磺酸化此幾丁聚糖，使形成磺酸化幾丁聚糖。 接著’提供一海藻酸鹽，並將此海藻酸鹽與磺酸化幾丁聚 糖溶於溶劑中’以配制成紡絲原液。其中’磺酸化幾丁聚 糖與海藻酸鹽的重量比約為1:1〜1:9。最後，利用濕式紡絲 法將紡絲原液中的磺酸化幾丁聚糖與海藻酸鹽紡成抗菌纖 5 200928024 維。 依照本發明另一實施例’提出一種紡絲原液之組成。 此紡絲原液包含海藻酸鹽、磺酸化幾丁聚糖與溶劑。續酸 化幾丁聚糖係由幾丁聚糖經磺酸化反應而得，其中幾丁聚 糖的重量平均分子量約為10,000〜50,00(^磺酸化幾丁聚糖 與海藻酸鹽的重量比約為1:1〜1:9。 依照本發明再一實施例’提出一種抗菌纖維。此抗菌 纖維包含海藻酸鹽與磺酸化幾丁聚糖。磺酸化幾丁聚糖係 由幾丁聚糖經確酸化反應而得，其中幾丁聚糖的重量平均 分子量約為10,000〜50,000。項酸化幾丁聚糖與海藻酸鹽的 重量比約為1:1〜1:9。 依照本發明又一實施例，提出一種抗菌織物，此抗菌 織物係由前述實施例所述之抗菌纖維織造而成。 本發明上述實施例所述之抗菌纖維或抗菌織物，對於 痤瘡桿菌與其他多種菌種皆具有良好的抗菌效果。 【實施方式】 抗菌鏃维芻作 第1圖係繪示依照本發明一實施例所述之抗菌纖維製 作方法的流程圖。首先，進行步驟11〇,提供一幾丁聚糖， 此幾丁聚糖的重量平均分子量約為1〇〇〇〇〜5〇〇〇〇。 接著’進行步驟120,將前述之幾丁聚糖進行磺酸化反 應’使形成磺酸化幾丁聚糖。磺酸化幾丁聚糖的方式可為 任何可行的方式，例如可使用氣磺酸C1HS03與幾丁聚糖進 行反應’以獲得磺酸化幾丁聚糖。 6 200928024 上述利用氯磺酸進行幾丁聚糖磺酸化反應之具體方式 如下所述。首先’將幾丁聚糖、氣磺酸與二曱基甲醯胺 (N，N-Dimethyl formamide; DMF)於預定溫度如 8〇〇c 下反應 預定時間如5小時。之後，再以例如NaOH溶液將前述溶 液之pH值調整至7。最後，再加入丙_溶液將確酸化幾丁 聚糖析出，並以甲醇清洗析出物，再乾燥析出物以獲得績 酸化幾丁聚糖。 請再參考第1圖，磺酸化幾丁聚糖製備完成後，可進 行步驟130,將磺酸化幾丁聚糖與一海藻酸鹽溶解於一溶劑 中，以配製成紡絲原液。其中’磺酸化幾丁聚糖與海藻酸 鹽的重量比約為1:1〜1:9。藉由前述海藻酸鹽的使用，可提 高抗菌纖維製程中的紡絲效果。海藻酸鹽例如可為海藻酸 鈉，海藻酸鈉的重覆單元例如可為式I或式II所示之'锋構 海藻酸鈉的分子量約為50,000〜150,000。溶劑例如可為& 以提高纖維製程之環保效果。 ’ ❷Textiles can be seen everywhere in everyday life. Among them, I often make progress with the advancement of technology, so that I want to wear 2 = good warmth function, and I also hope that the fabric itself has the effect of antibacterial. <Function In addition to the user's desire for the fabric to have the aforementioned additional functions, the quotient itself also desires to increase the added value and application of the fabric by fabricating the fabric having the aforementioned antibacterial effect. Improvements in the use of fibers for textile weaving in order to achieve the above objectives are one of the current efforts of manufacturers. SUMMARY OF THE INVENTION Embodiments of the present invention provide a method for producing an antimicrobial fiber and an antimicrobial fiber produced thereby. According to an embodiment of the present invention, a method for producing an antimicrobial fiber is proposed. First, a chitosan having a weight average molecular weight of about 10,000 to 50 000 is provided. This chitosan is then sulfonated to form a sulfonated chitosan. Next, an alginate is supplied and this alginate is dissolved in a solvent with a sulfonated chitosan to prepare a spinning dope. Wherein the weight ratio of the sulfonated chitosan to the alginate is from about 1:1 to about 1:9. Finally, the sulfonated chitosan and the alginate in the spinning dope are spun into an antibacterial fiber by a wet spinning method. According to another embodiment of the present invention, a composition of a spinning dope is proposed. This spinning dope contains alginate, sulfonated chitosan and a solvent. The acidified chitosan is obtained by sulfonation reaction of chitosan, wherein the weight average molecular weight of chitosan is about 10,000~50,00 (the weight ratio of sulfonated chitosan to alginate) It is about 1:1~1:9. According to still another embodiment of the present invention, an antibacterial fiber is proposed. The antibacterial fiber comprises alginate and sulfonated chitosan. The sulfonated chitosan is chitosan. The acidification reaction is obtained, wherein the chitosan has a weight average molecular weight of about 10,000 to 50,000. The weight ratio of the acidified chitosan to the alginate is about 1:1 to 1:9. In an embodiment, an antibacterial fabric is provided which is woven from the antibacterial fiber described in the foregoing embodiments. The antibacterial fiber or the antibacterial fabric of the above embodiment of the present invention has good properties for the acne bacillus and various other species. [Embodiment] The first embodiment of the present invention is a flow chart of a method for preparing an antibacterial fiber according to an embodiment of the present invention. First, step 11 is performed to provide a chitosan. The weight of this chitosan The average molecular weight is about 1 〇〇〇〇 5 〇〇〇〇. Next, 'Step 120 is performed to sulfonate the chitosan described above' to form a sulfonated chitosan. The sulfonated chitosan The method may be any feasible manner, for example, the reaction of gas sulfonic acid C1HS03 with chitosan may be used to obtain a sulfonated chitosan. 6 200928024 The specific manner of the above-mentioned chitosan sulfonation reaction using chlorosulfonic acid As described below, first, the chitosan, gas sulfonic acid and N, N-Dimethyl formamide (DMF) are reacted at a predetermined temperature such as 8 〇〇c for a predetermined time such as 5 hours. Further, the pH of the solution is adjusted to 7 by, for example, a NaOH solution. Finally, the addition of the propylene solution causes the acidified chitosan to be precipitated, and the precipitate is washed with methanol, and the precipitate is further dried to obtain an acidified chitosan. Referring to FIG. 1 again, after the preparation of the sulfonated chitosan is completed, step 130 may be performed to dissolve the sulfonated chitosan and an alginate in a solvent to prepare a spinning dope. 'Sulphonated chitosan with alginate The weight ratio is about 1:1~1: 9. The spinning effect in the antibacterial fiber process can be improved by the use of the aforementioned alginate. The alginate can be, for example, sodium alginate or a repeating unit of sodium alginate. For example, the molecular weight of the sodium sulphate of the formula I or the formula II may be about 50,000 to 150,000. The solvent may be, for example, an environmentally friendly effect of the fiber process.
(II) 原液中的 接著進行步驟140 ’利用紡織技術將上述紡絲 7 200928024 飧酸化幾丁聚糖與海藻酸鹽紡成抗菌纖維。上述之紡織技 術可為/最式紡絲法。具體而言，上述之紡織方式可為將紡 絲原液接觸—成型液，以於成型液中形成抗菌纖維，其中 成型液中可包含鈣離子，以置換海藻酸鹽如海藻酸鈉中的 納離子。 ' 性質測談 Q 依照本發明上述實施例所述，分別製作磺酸化幾丁聚 糖與海藻酸鹽重量比為1 ·· 1、1:2、1 ..4與1:9之紡絲原液， 並進一步紡成抗菌纖維。其中’所選用之幾丁聚糖的重量 平均分子量約為21，000,磺酸化幾丁聚糖之磺酸化程度約 為62 % (由元素分析儀測得）^前述製作所得之抗菌纖維分 別纺成不織布，以進行後續抗菌測試、纖維毒性與皮膚安 定性測試。 ⑩ :痤瘡桿菌接觸式抗菌測詖 為測試本發明實施例所製成之抗菌纖維針對痤瘡桿菌 之殺菌效果，係進行痤瘡桿菌接觸式抗菌測試，並將測試 結果列於表一。其中，痤瘡桿菌為造成面皰產生之主要細 菌之一，因此在表一中亦同時以市售治療面皰之產品做對 照例。 上述接觸式抗菌測試的方式係為將少量痤瘡桿菌與測 試樣品混合，置於無菌塑膠培養皿中。之後再倒入45〜50 0C 的培養基（生鮮豬肉萃取液+—半濃度的LB培養基），於厭 氧缸中培養3〜4天，觀察痤瘡桿菌的生長情形。 200928024 表一痤瘡桿菌接觸式抗菌測試 抗菌纖維 (績酸化幾丁聚糖與海藻酸納重量比） 市售品 控制組 1:1 1:2 1:4 1:9 康痘 愛可凝膠 生菌數 50,000 1600 2000 2400 2600 23,000 4,000 殺菌率 — 96.8 % 96% 95.2 % 94.8 % 54% 92% 殺菌率：（控制組生菌數-測試樣品生菌數）/控制組生菌數 由表一可知，本發明實施例所述之抗菌纖維對於痤瘡 桿菌的殺菌率可達90 %以上，具有良好之殺菌效果，可用 以做為治療面疱用之貼合敷材。相較於市售之面疱治療敷 材，本發明實施例所述之抗菌纖維具有更佳之抗菌效果。 抗菌測試二：L1902-1998定量法 為測試本發明實施例所製成之抗菌纖維的抗菌效果， 係依照日本工業標準（Japanese. Industrial Standards，JIS ) L1902-1998定量法進行抗菌效果分析。其中，所使用之試 驗菌種為金黃色葡萄球菌（ATCC 6538P)、肺炎桿菌（ATCC 4352)、綠膿桿菌（ATCC 10145)及白色念珠菌（ATCC 18814)，測試結果分別列於表二〜表五。 200928024 表二抗菌纖維之抗菌效果（金黃色葡萄球菌） 金黃色葡萄球菌 白棉布 抗菌纖維 磺酸化幾丁聚糖與海藻酸鈉重量比 市售商品 1:1 1:2 1:4 1:9 愛可凝膠 康痘 植菌濃度 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 Ma 2.6E+4 — --- --- — _一 Mb 1.6E+6 — --- —- —- — 一- Me <20 <20 <20 <20 <20 <20 細菌成長活性值 1.8 — — — 一 ---- — 抑菌值 4.9 4.9 4.9 4.9 4.9 4.9 殺菌值 --- 3.1 3.1 3.1 3.1 3.1 3.1 1. Ma為未加工樣品，經0小時立即沖刷後之菌數 2. Mb為未加工樣品，經18~24小時培養後箇數 3. Me為加工樣品，經18〜24小時培養後菌數。 4. 細菌成長活性值=l〇g(Mb/Ma);若>1.5,表示實驗有效 5. 抑菌值=log(Mb/Mc);殺菌值=log(Ma/Mc) 表三抗菌纖維之抗菌效果（肺炎桿菌） 肺炎桿菌 白棉布 抗菌纖維 磺酸化幾丁聚糖與海藻酸納重量比 市售商品 1:1 1:2 1:4 1:9 愛可凝膠 康痘 植菌濃度 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 Ma 2.6E+4 --- —— Mb 3.2E+7 --- 一 —— 一 一 Me <20 <20 <20 <20 <20 <20 細菌成長活性值 3.1 一 --- 一 一 --- 一 抑菌值 —- 6.2 6.2 6.2 6.2 6.2 6.2 殺菌值 一_ 3.1 3.1 3.1 3.1 3.1 3.1 10 200928024 表四抗菌纖維之抗菌效果（綠膿桿菌） 綠膿桿菌 白棉布 抗菌纖維 磺酸化幾丁聚糖與海藻酸納重量比 市售商品 1:1 1:2 1:4 1:9 愛可凝膠 康痘 植菌濃度 0.7E+5 0.7E+5 0.7E+5 0.7E+5 0.7E+5 0.7E+5 0.7E+5 Ma 1.5E+4 --- — — --- — Mb 1.5E+7 —- — 一 一 ——— --- Me — <20 <20 <20 <20 <20 <20 細菌成長活性值 3.0 —- — --- --- 一 抑菌值 --- 5.9 5.9 5.9 5.9 5.9 5.9 殺菌值 --- 2.9 2.9 2.9 2.9 2.9 2.9 表五抗菌纖維抗菌效果（白色念珠菌） 白色念珠菌 白棉布 抗菌纖維 磺酸化幾丁聚糖與海藻酸納重量比 市售商品 1:1 1:2 1:4 1:9 康痘 植菌濃度 1.1E+5 1.1E+5 1.1E+5 1.1E+5 1.1E+5 1.1E+5 Ma 2.2E+4 一 一 … --- Mb 7.3E+5 — 一 一 — Me —- 2.2E+3 <20 1.4E+4 <20 <20 細菌成長活性值 1.52 — -一 --- —- 抑菌值 2.5 4.6 1.7 4.6 4.6 殺菌值 一 1.0 3.0 0.2 3.0 3.0 請先參考表二〜表四，本發明實施例所述之抗菌纖維， 11 200928024 在測試中所獲得之抑菌值[l〇g(Mb/Mc)]約為4_9〜6.2，殺菌 值[log(Ma/Mc)]約為2.9〜3.1。依據曰本纖維製品新機能評 價協議會（JAFET)的抗菌標準，當抑菌值大於2.2，表示測 武樣本具有抑菌效果。當殺菌值大於〇，表示測試樣本具有 殺菌效果》因此，可推知上述抗菌纖維對於金黃色葡萄球 菌、肺炎桿菌與綠膿桿菌皆具有良好的抑菌與殺菌效果， 且其效果與目前市售產品相當，使發明實施例所述之抗菌 纖維具商品化之潛能。 請再參考表五，本發明實施例所述之抗菌纖維對於白 色念珠菌具有殺菌效果，且部份測試樣品的殺菌效果與市 售品相當。此外，經由調整抗菌纖維中磺酸化幾丁聚糖與 海藻酸鈉的比例，亦可對白色念珠菌產生與市售品相當之 抑菌效果。 抗菌纖維之細跑毒性泪彳結 為測試本發明上述實施例所述之抗菌纖維與細胞接觸 時是否會造成細胞之死亡，係採用ASTM 813·83的規範’ 進行細胞毒性測試。首先，將L929 fibroblast細胞培養在 polystyrene cell-culture dish (直徑 ό cm)。當細胞數達到 預定?農度時’將抗菌纖維製成之不織布置於dish中央（直 仏1.1 cm)繼續培養24小時。之後以2°/。crystal violet· 做細胞染色，以巨觀觀察細胞之死亡情形。 經由觀察與抗菌纖維接觸區域之細胞的生長形態判斷 抗菌織維是否具毒性’並將測試結果列於表六β若與抗菌 纖維接觸區域之細胞的生長情形未受影 響，則推斷抗菌纖 12 200928024 維無細胞毒性。反之，若與抗菌纖維接觸區域之細胞呈現 死亡之情形’則推斷抗菌纖維具細胞毒性。 表六抗菌纖維毒性測試 磺酸化幾丁聚糖與 海藻酸納之會眚出 1:1 1:2 1:4 1:9 1:0 細胞毒性 — ... 無 無 無 無 有 ❹ 由表六可知純的磺酸化幾丁聚糠具有細胞毒性，可能 會抑制細胞之分裂與生長。當添加適當比例之海藻酸鈉與 續酸化幾丁聚糖共紡成抗菌纖維時，可使所製成之抗菌纖 維不具細胞毒性，藉此，可製作貼敷於人體表面之貼敷材 料。 全性測試(II) In the stock solution, the above-mentioned spinning 7 200928024 decanolated chitosan and alginate are spun into an antibacterial fiber by a textile technique. The above textile technique can be / most spinning. Specifically, the spinning method may be that the spinning dope is contact-forming liquid to form an antibacterial fiber in the molding liquid, wherein the molding liquid may contain calcium ions to replace the nano ions in the alginate such as sodium alginate. . 'Quality Test Q According to the above embodiment of the present invention, the spinning dope having a weight ratio of sulfonated chitosan to alginate of 1 ··1, 1:2, 1..4 and 1:9, respectively, is prepared. And further spun into antibacterial fibers. The weight average molecular weight of the selected chitosan is about 21,000, and the degree of sulfonation of the sulfonated chitosan is about 62% (measured by an elemental analyzer). Non-woven for subsequent antimicrobial testing, fiber toxicity and skin stability testing. 10: Acne bacillus contact antibacterial test To test the bactericidal effect of the antibacterial fiber prepared in the examples of the present invention against acne bacillus, the contact bacteriostatic test of acne bacillus was carried out, and the test results are shown in Table 1. Among them, acne bacillus is one of the main bacteria causing blistering, so in Table 1, the products for the treatment of blister are also used as a rule. The above contact type antibacterial test is carried out by mixing a small amount of acne bacteria with a test sample and placing it in a sterile plastic petri dish. Then, a medium of 45 to 50 ° C (raw pork extract + half-concentration LB medium) was poured, and cultured in an anaerobic tank for 3 to 4 days to observe the growth of acne bacteria. 200928024 Table 1 Acne bacillus contact antibacterial test antibacterial fiber (weight ratio of acidified chitosan to sodium alginate) Commercial control group 1:1 1:2 1:4 1:9 Acne love gel bacteria Number 50,000 1600 2000 2400 2600 23,000 4,000 Sterilization rate - 96.8 % 96% 95.2 % 94.8 % 54% 92% Sterilization rate: (control group bacteria number - test sample bacteria number) / control group bacteria number is shown in Table 1, The antibacterial fiber according to the embodiment of the invention has a sterilization rate of more than 90% for the acne bacillus, and has a good bactericidal effect, and can be used as a affixing material for treating blister. The antibacterial fiber of the embodiment of the present invention has a better antibacterial effect than the commercially available blister treatment dressing. Antibacterial Test 2: L1902-1998 Quantitative Method To test the antibacterial effect of the antibacterial fiber produced in the examples of the present invention, the antibacterial effect was analyzed according to the Japanese Industrial Standards (JIS) L1902-1998 quantitative method. Among them, the test strains used were Staphylococcus aureus (ATCC 6538P), Klebsiella pneumoniae (ATCC 4352), Pseudomonas aeruginosa (ATCC 10145) and Candida albicans (ATCC 18814). The test results are listed in Table 2 to Table Fives. 200928024 Table 2 Antibacterial effect of antibacterial fiber (Staphylococcus aureus) Staphylococcus aureus white cotton cloth Antibacterial fiber sulfonated chitosan and sodium alginate weight ratio Commercial goods 1:1 1:2 1:4 1:9 Love Gel-like acne bacteria concentration 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 Ma 2.6E+4 — --- --- — _ A Mb 1.6E+6 — --- — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 Bacteriostatic value 4.9 4.9 4.9 4.9 4.9 4.9 Sterilization value --- 3.1 3.1 3.1 3.1 3.1 3.1 1. Ma is an unprocessed sample, the number of bacteria immediately after 0 hours of washing 2. Mb is an unprocessed sample, after 18 to 24 hours After the cultivation, the number of mash was 3. Me, which was a processed sample, and the number of bacteria was cultured after 18 to 24 hours. 4. Bacterial growth activity value = l〇g (Mb/Ma); if >1.5, it means that the experiment is effective 5. Antibacterial value = log (Mb / Mc); Sterilization value = log (Ma / Mc) Table 3 Antibacterial fiber Antibacterial effect (K. pneumoniae) Pneumococcal white cotton cloth antibacterial fiber sulfonated chitosan and sodium alginate weight ratio commercial goods 1:1 1:2 1:4 1:9 Ai Ke gel Kang pox concentration of 1.3 E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 1.3E+5 Ma 2.6E+4 --- —— Mb 3.2E+7 --- One - one one Me <20 <20 <20 <20 <20 <20 Bacterial growth activity value 3.1 One--- One---- One antibacterial value-- 6.2 6.2 6.2 6.2 6.2 6.2 Sterilization value _ 3.1 3.1 3.1 3.1 3.1 3.1 10 200928024 Table 4 Antibacterial effect of antibacterial fiber (Pseudomonas aeruginosa) Pseudomonas aeruginosa white cotton cloth antibacterial fiber sulfonated chitosan and sodium alginate weight ratio commercial goods 1:1 1:2 1:4 1:9 Ai Ke gel acne bacteria concentration 0.7E+5 0.7E+5 0.7E+5 0.7E+5 0.7E+5 0.7E+5 0.7E+5 Ma 1.5E+4 --- — — --- — Mb 1.5E+7 —- — 一一———— --- Me — <20 <20 <20 <20 <20 <20 Bacterial Growth Value 3.0 —- -- --- --- One antibacterial value --- 5.9 5.9 5.9 5.9 5.9 5.9 Sterilization value --- 2.9 2.9 2.9 2.9 2.9 2.9 Table 5 Antibacterial effect of antibacterial fiber (Candida albicans) White Candida albicans Cotton cloth antibacterial fiber sulfonated chitosan and sodium alginate weight ratio commercial goods 1:1 1:2 1:4 1:9 Acne bacteriophage concentration 1.1E+5 1.1E+5 1.1E+5 1.1E+ 5 1.1E+5 1.1E+5 Ma 2.2E+4 One by one... --- Mb 7.3E+5 — One to one — Me —- 2.2E+3 <20 1.4E+4 <20 <20 Bacteria Growth activity value 1.52 - -1 - - - - - Antibacterial value 2.5 4.6 1.7 4.6 4.6 Sterilization value - 1.0 3.0 0.2 3.0 3.0 Please refer to Table 2 to Table 4, the antibacterial fiber according to the embodiment of the present invention, 11 200928024 The bacteriostatic value [l〇g(Mb/Mc)] obtained in the test was about 4-9 to 6.2, and the bactericidal value [log(Ma/Mc)] was about 2.9 to 3.1. According to the antibacterial standard of JAFET, when the antibacterial value is greater than 2.2, it means that the test sample has antibacterial effect. When the bactericidal value is greater than 〇, it means that the test sample has a bactericidal effect. Therefore, it can be inferred that the above antibacterial fiber has good antibacterial and bactericidal effects against Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa, and the effect thereof is compared with the currently marketed product. In contrast, the antibacterial fibers described in the inventive examples have the potential to be commercialized. Referring to Table 5 again, the antibacterial fiber according to the embodiment of the present invention has a bactericidal effect on Candida albicans, and the bactericidal effect of some of the test samples is comparable to that of the commercial product. Further, by adjusting the ratio of sulfonated chitosan to sodium alginate in the antibacterial fiber, Candida albicans can also produce a bacteriostatic effect comparable to that of a commercially available product. The toxic toxic tear layer of the antibacterial fiber. To test whether the antibacterial fiber of the above-mentioned embodiment of the present invention causes cell death when it comes into contact with cells, the cytotoxicity test is carried out using the specification of ASTM 813·83. First, L929 fibroblast cells were cultured in a polystyrene cell-culture dish (diameter ό cm). When the number of cells reached the predetermined agronomic degree, the non-woven fabric made of the antibacterial fiber was placed in the center of the dish (straight 1.1 cm) and culture was continued for 24 hours. After 2°/. Crystal violet· Perform cell staining to observe the death of cells in a giant way. It is judged whether the antibacterial texture is toxic by observing the growth morphology of the cells in contact with the antibacterial fiber' and the test results are listed in Table 6. If the growth of the cells in the contact area with the antibacterial fiber is not affected, it is inferred that the antibacterial fiber 12 200928024 Vitamins are cytotoxic. On the other hand, if the cells in contact with the antibacterial fiber show a state of death, it is presumed that the antibacterial fiber is cytotoxic. Table 6 Antibacterial fiber toxicity test sulfonated chitosan and sodium alginate will 1:1 1:2 1:4 1:9 1:0 cytotoxicity - ... no no no no ❹ by Table 6 It can be seen that pure sulfonated chitosan is cytotoxic and may inhibit cell division and growth. When an appropriate ratio of sodium alginate and a continuous acidified chitosan are added to form an antibacterial fiber, the prepared antibacterial fiber can be made non-cytotoxic, whereby a patch applied to the surface of the human body can be produced. Full test
為測試本發明實施例所述之抗菌纖維製作成貼敷材料 g 時是否會對皮廣產生過敏之情形，係採用ISO ^993-10-2002的規範’進行皮膚刺激性與敏感性測試。測 成結果分別列於表七與表八。 13 200928024 表七皮膚刺激性測試 對照組 試驗組 動物隻數 3 3 塗抹物質 注射用蒸餾水 抗菌織維不織布 紅斑與痂皮 0/3 0/3 浮腫 0/3 0/3 n/n:具有臨床症狀發生之動物隻數/每組動物隻數 表八皮膚敏感性測試 負對照組 正對照組 試驗組 性別 雄 雄 雄 動物隻數 5 5 10 紅斑 0/5 5/5 0/10 浮腫 n/n :且古祐迮θ 0/5 4L· aL Ar 0/5 0/10 n/n:具有臨床症狀發生之動物隻數/每組動物隻數 ❹ 由表七與表八中可知’本發明實施例所述之抗菌纖維 均無皮廣敏感性與刺激性，可用以製作貼敷於人體表面之 貼敷材料。 雖然本發明已以實施例揭露如上，然其並非用以限定 本發明，任何熟習此技藝者，在不脫離本發明之精神和範 圍内，當可作各種之更動與潤飾，因此本發明之保護範圍 當視後附之申請專利範圍所界定者為準。 【圖式簡單說明】 200928024 為讓本發明之上述和其他目的、特徵、優點與實施例 能更明顯易懂，所附圖式之詳細說明如下： 第1圖係繪示依照本發明一實施例所述之抗菌纖維製 作方法的流程圖。 【主要元件符號說明】 110、120、130、140 :步驟In order to test whether the antibacterial fiber according to the embodiment of the present invention is made into a patch material g, whether it is allergic to the skin, the skin irritation and sensitivity test is carried out according to the specification of ISO ^993-10-2002. The results of the measurements are shown in Tables 7 and 8. 13 200928024 Table 7 skin irritation test control group of experimental animals only 3 3 smear substance injection with distilled water antibacterial woven non-woven erythema and suede 0/3 0/3 edema 0/3 0/3 n/n: with clinical symptoms The number of animals that occurred/only the number of animals in each group. Table 8 Skin sensitivity test Negative control group Positive control group Test group Sex male and male animals only 5 5 10 Red spot 0/5 5/5 0/10 Puffy n/n :古 迮 迮 θ 0/5 4L· aL Ar 0/5 0/10 n/n: only the number of animals with clinical symptoms/number of animals per group ❹ From Tables 7 and 8, it is known that the present invention The antibacterial fiber has no skin sensitivity and irritancy, and can be used to make a patch applied to the surface of the human body. Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and the present invention can be modified and retouched without departing from the spirit and scope of the present invention. The scope is subject to the definition of the scope of the patent application attached. BRIEF DESCRIPTION OF THE DRAWINGS The above and other objects, features, advantages and embodiments of the present invention will become more <RTIgt; A flow chart of the method for producing an antimicrobial fiber. [Main component symbol description] 110, 120, 130, 140: steps
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