TW200914814A - Method and apparatus for estimating the content of an analyte in a multi-layer medium - Google Patents

Abstract

The invention relates to an apparatus for non-invasively estimating the content of an analyte in a multi-layer medium, based on spectral measurements. The apparatus comprises: a radiation source adapted to generate spectrums of electromagnetic radiation and to transmit the spectrums to the multi-layer medium; a detector adapted to detect spectrums of reflected radiation from the multi-layer medium and to generate detection signals representing the detected radiation; and a data processing means adapted to derive from the detected signals a plurality of quantities representing the effective attenuation coefficients of the multi-layer medium, and to estimate the content of the analyte from the plurality of quantities, based on a regression model.; As the estimation of the content of the analyte is based on a plurality of quantities representing the effective attenuation coefficients, which change with the incident wavelengths of the spectrum of the electromagnetic radiation and the source-detector distance between irradiation areas and detection areas, the invention improves the precision of the estimation of the analyte content in a multi-layer medium.

Description

200914814 IX. INSTRUCTIONS: TECHNICAL FIELD OF THE INVENTION The present invention relates to non-intrusive monitoring of analytes in a medium. Specifically, the present invention relates to an apparatus and method for non-invasively estimating the analyte content in a multilayer medium based on the use of near-infrared light energy to illuminate a multilayer medium, such as skin tissue. [Prior Art] Near-infrared tissue spectroscopy is a promising non-invasive technique based on measuring the near-infrared energy in the wavelength range of 700-2500 nm to illuminate tissue. Energy is focused on the area of the skin and propagates according to the scattering and absorption properties of the skin tissue. Thus, the energy of reflection or transmission is detected' and provides information about the volume of tissue encountered. The prior art document WO 20070605 83 discloses a method for non-invasive measurement of the concentration of at least one analyte in a turbid medium having an effective attenuation coefficient of ~(A). According to W〇 2007060583, the scattering of light in a turbid medium increases the effective optical path length, resulting in an effective attenuation coefficient that is greater than the absorption coefficient. The attenuation derived from the transmission or reflection measurements of the spectroscope on a turbid medium is not proportional to the sum of the absorption coefficients of the components. Thus 'simple linear regression analysis becomes ineffective. The method in W〇2〇〇7〇6〇583 includes the steps of: illuminating the turbid medium with a plurality of radiation sources, using a spectrum of electromagnetic radiance; detecting the turbid medium reflection by means of a plurality of detectors The reflected spectrum of the electromagnetic light is such that the reflectance spectrum is detected at at least two different source-detector distances, such that the distance is selected such that the A, 2 >>1/~; - Detector distance (θ 2) corresponds to the opposite of the 125154.doc 200914814 relative change in the number of ^, -------, and derives from the first amount represents the effective = coefficient ~ _ two And determining the concentration based on the second amount. The method of extracting π is effective to make the measured spectrum linear in various turbid media* various knives, which allows a simple linear regression to be arbitrarily selected by the effective medium thickness, and the effective absorption coefficient can be replaced. Implemented by definition.

The method disclosed in WO 2007060583 does not solve the problem when using: to estimate the analyte in the multi-layer medium of the living body (example & water in the skin tissue) (the situation is very complicated) )The problem. First, the thickness of the dermis is not constant. In addition to the dehydrated material, factors contributing to skin thickness may include age, menstruation, and the like. Subcutaneous fat cells contain less water than dermis, while the thickness of the fat layer has a high degree of personality. These complexities should be carefully considered; otherwise significant errors will be introduced in the measurements. [Summary of the Invention]

Multi-layer media is estimated for a number of purposes. It is an object of the present invention to provide an apparatus for non-invasive analyte content which enhances quantification. To this end, the present invention provides for non-invasive estimation of analytes in a multilayer medium based on spectrometry. a device comprising: a radiation source 'for generating an electromagnetic light spectrum corresponding to a plurality of individual wavelengths and adapted to emit the spectrum to the multilayer medium; a detector for detecting reflection from the multilayer medium The spectrum is generated to generate a detection signal indicating the compensated Korean shot, #中' is disposed on the multilayer medium with the irradiation area of the radiation source and the phase area of the detector, so as to be 125154.doc 200914814 The incident wavelength, πο is generated and detected by at least two different sources, the source-detector distance, which is defined as the distance between the illumination area and the detection area, and the respective sources - the distance of the detector is much larger than the multi-layer; the reciprocal of the effective mitigation coefficient of 丨 buy and the data processing device are used to derive the complex attenuation coefficient of the multilayer medium from the detected signal An amount, based on the regression model and the plurality of the complex from the estimated amount of content A analytes. The present invention improves the quantization accuracy by estimating the analyte 罝' based on a table of ineffective attenuation systems and a plurality of quantities, the t number of which varies with the wavelength of the human radiation of the electromagnetic radiation spectrum. In one embodiment of the invention, the source-detector distance is adjustable such that the depth of radiation penetration between the illuminated area and the detected area reaches a different layer in the multilayer medium. The present invention further provides an estimate of the accuracy of analytes in multilayer media by considering contributions from different layers. Another object of the present invention is to provide a method for non-invasively estimating the content of an analyte in a multilayer medium, which method improves the quantization accuracy. To this end, the present invention provides a method for non-invasively estimating the amount of an analyte in a multilayer medium based on spectrometry, the method comprising the steps of: using electromagnetic radiation generated by a radiation source corresponding to a plurality of incident wavelengths A spectrum is used to illuminate the multilayer medium; a detector is used to detect a corresponding signal representing a spectrum reflected from the multilayer medium' to target each of the plurality of incident wavelengths, based on 125154.doc 200914814 two different sources-detection The detector distance is detected by the distance of the detector, wherein the source-detector distance is selected to be much larger than the multi-layer medium; the reciprocal of the attenuation coefficient; the signal towel derived from the detected signal and the complex personal wavelength And a source-predator distance corresponding to and representing a plurality of quantities of the effective attenuation coefficient of the multilayer medium; and estimating the content of the analyte based on the plurality of quantities based on the regression model. Furthermore, the present invention provides a computer program product to be loaded by a computer device, comprising a fingerprint for estimating a flaw in an analyte based on a spectral measurement, the computer device comprising a processing unit and a memory, a computer The program product, after being loaded, provides the processing unit with the ability to perform the following tasks: Obtaining speculative data indicative of the spectrum of radiation reflected from the plurality of incident wavelengths and reflected from the multilayer medium; a plurality of quantities of effective attenuation coefficients of the multilayer medium that do not correspond to a plurality of incident wavelengths and source-detector distances; and based on the regression model, the analyte content is estimated based on the plurality of quantities. Modifications and alterations of the devices, methods, and computers that are described in the <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; [Embodiment] Fig. 1 shows a schematic illustration of a non-invasive biological analyte monitor according to the present invention. The non-invasive biological analyte monitor includes: a table 2, and a detector control positioned on the multilayer medium 4 on the boundary 5 of the latter. 125154.doc -10- 200914814 For example, in the case of a dehydration monitor for skin tissue Next, the multilayer medium 4 is the skin of the patient. The skin tissue 4 includes three layers: epidermis 4-1, dermis 4-2, and subcutaneous tissue 4-3 (fat, etc.). A coupling agent, such as a highly dispersed gel (not shown), may be provided between the detector 3 and the boundary $. The monitor console 2 includes a light source (e.g., a lamp), and the light of the lamp 6 is focused via the optical device 7 on the waveguide fiber bundles 8_1, 8_2, 8-3, 8-4, 8-5, and 8-6 (source fiber bundle). The waveguide fiber bundles extend substantially to the boundary of the multilayer dielectric 4. A shadow switch 9 is provided between the optical device 7 and the excitation ends 8c of the waveguide fiber bundles 8_丨, 8_2, 8-3, 8-4, 8-5 and 8-6, which can be controlled for one excitation The lighter of the 8_丨, 8_2, 8_3, 8_4, 8_5, and 8_6 will perform optical operations in the wavelength range of 930 to 1750 nm for the dehydration monitor. Light having a shorter wavelength outside the range may be filtered by a semiconductor filter formed of a Si substrate (not shown) disposed in a path between the lamp 6 and the excitation end 8c of the waveguide fiber bundle. In the detection path, the non-invasive biological analyte monitor 1 further includes a waveguide fiber bundle 10 (detection fiber) that views the first end and the detector and thereby extends substantially to the boundary of the multilayer medium 4 5. The waveguide fiber bundle 1 is connected to a monochromator 11it included in the monitor console 2. The pre-colorator 11 is operatively coupled to the detector array 12, which is further coupled to the electronic single core. The electronic single (four) advance step includes a data processing list: i. 5 the material at the bedding material is suitable for the output of the controller array 12: 乍 - In addition, the 151 visual device console 2 is operatively connected to the output device (: such as display U) and input device 16 (such as CD_R〇M driver and network). The input device 16 is suitable for respectively selling the appropriate information 125154.doc 200914814 carrier; quality or data from the computer network The stream is used to provide the data processing device 15 with a computer program product having executable instructions, as will become apparent hereinafter.

Figure 2 is a schematic illustration of the first configuration of the waveguide fiber in the detector 3 of the non-invasive biological analyte monitor according to the Figure. In Fig. 2, the detector 3 is shown viewed from below, in Fig. 2, the side of the detector 3 faces the boundary 5 of the multilayer "shell 4. In the illustrated embodiment the source bundle 8_丨, 8 3, 8-4, 8-5, and 8-6 are concentrically arranged around the center detector fiber 1 以便 so as to be between the source radiation area and the detection areas A, A, A, 八, 八, and A The source-detector distance is different from the distance between the source beams 8_丨, 82, 8_3, 8-4, 8-5, and 8-6 and the center detector fiber 1〇. a multimode fiber having a core diameter d. The source fiber bundles 8-1, 8-2, 8-3, 8-4, 8-5, and 8-6 are selected such that the source-detector distance is much larger than the multilayer The reciprocal of the effective attenuation coefficient of the medium, such as A » 1 / #, especially having a multiple of about 1 。. In addition, each source bundle includes a circular pattern surrounding the center detector fiber 1 〇 a plurality of multimode fibers (not explicitly shown). The radius of the ring should be large enough for A · 4 » 1 ' but small enough to retrieve as much of the signal as possible. In operation, lamp 6 is used as the primary source of radiation, According to the shadow In the switching state of the switch 9, the multilayer dielectric 4 is irradiated via the waveguide bundles 8-丨, 8_2, 8_3, 8_4, 8_5 and 8_6. This is based on the source beams 8-1, 8- which are sequentially excited on the boundary 5 of the multilayer dielectric 4. 2, 8-3, 8_4, 8-5, and 8-6 configurations create an illumination area. The light that is absorbed and scattered, and the illumination is attenuated in the multilayer medium 4 125154.doc -12- 200914814 and ~ different directions Reflected therefrom. Part of the light reflected by the multilayer medium is collected by the bundle 1 'the bundle 10 is connected to the monochromator η and the array 12 is used to measure the reflection spectrum. For example, the reflection from the multilayer medium* The intensity reflection coefficient or reflectance of the light shot. The output of the 35 detector array 12 is transmitted to the electronic unit 13, which will be amplified, processed by means of a data processing device, and displayed to the user via the display 14. For example, by providing the appropriate program code for execution by the data processing device by means of the input device 16 and the medium 17, the data processing device 15 is adapted to operate on the output signal of the detector array 12 to derive an effective representation of the multilayer medium. The amount of the subtraction factor. The detailed method for deriving the amount of effective attenuation coefficient representing the multilayer medium is described in Reference! Assume that 仏 denotes the total absorption coefficient and ～ denotes the reduced scattering coefficient, based on the derivation of the reference ,, especially Combining Equation 7, Equation u and Equation 12 in 2__3, the amount of coffee is defined as: 5(Λ,ρ) = d\n{R(X,p)) dp 3ΜαμΙ

(2) where scattering (by decreasing the "scattering coefficient 〆 table #) phase # is absorbed by the component (represented by the absorption coefficient of the heart), and the source_detector distance p is sufficiently large, for example, satisfying p, »1/~ . In order to calculate the umbrella, P), two spectra with incident wavelengths are illuminated at the first-irradiation region and the second illumination region, and are composed of a monochromator and then by 125154.doc -13- 200914814 in two different The source-detector distance detectors 12 from A and A (with a difference Λ) measure the corresponding reflection spectrum in the detection region. The derivation of ln(/?(;l)) is implemented according to the following equation: = ln(male,))-in(male)) dp ~—'~ (3) Correspondingly, according to Equation 1 and Equation 3 To calculate the amount; 1,, outside). There are many factors that affect the effective attenuation coefficient ~ and thus affect the amount of outsiders". On the one hand, the absorption coefficient varies with the incident wavelength, especially at some characteristic wavelengths. On the other hand, the source-detector The distance is related to the depth of photon penetration that affects the absorption of the spectrum. The larger the source-detector, the higher the probability that the photon will reach the deeper layer of the multilayer medium, and vice versa. In addition, the anatomical structure of the multi-media in the living body will be photons. The behavior of the human skin tissue, for example, in the epidermis, dermis and subcutaneous tissue, the water contains different sputum. When estimating the water content in the skin tissue, the important 疋 carefully define the source-detector distance, Thereby adjusting the depth of radiation penetration between the illumination area and the detection area to reach different layers of the skin tissue. By defining and adjusting the incident wavelength of the incident spectrum and the source-detector distance, the representation and each incident spectrum can be obtained. The source-detector distance corresponds to the amount of effective attenuation coefficient. The input wavelength is usually selected so that each incident wavelength corresponds. The characteristic line in the spectrum of the reflected radiation from the multilayer medium. In order to reach the three layers of human skin tissue, it can be from 93〇_1〇〇〇nm, 115〇_125〇nm and 14〇〇-15〇0 nm. Select the recommended incident wavelength 44 and 岑 select the reference wavelength Λ for example 780 nm for reference. At the same time, the source-detection 125154.doc -14- 200914814 distance 丨P · D lf ( ^ 1 piece, M and Each of the two, for example, 'parent two adjacent fiber bundles can be grouped together to generate a reflection of the first signal pair, ', and then used to calculate 屯, p)., , __〇5l }253 , . = u;; , return to the f model to estimate the water content, &amp; is expressed as the following equation: (4) The center indicates the number of incident wavelengths that can be estimated based on the calibration adjustment and determination of the regression parameters. 'The number of pairs of detectors used for measurement is W. Indicates the incident wavelength used for reference. The absorption contributions from different sources are weighted to adjust the hydration state. It should be noted that the wavelength of the human shot The choice is based on experience. In order to make the return correctly It may have a wavelength that interferes with the analyte. For example, the analyte of interest has a value of ♦ at the human wavelengths v々 and &amp; the interference analyte has absorption at {and 乂4' and the regression coefficient should be negative at 々. And therefore should be included in the excitation spectrum. Due to the different scattering coefficients in different layers, dermis and subcutaneous fat tissue, 'significant changes are expected when photons pass through the boundary between different layers of the multilayer structure. T is used This change in the boundary defines the regression coefficient in the calibration. Specifically, 'measurement can be performed on tissues, phantoms or specimens with different levels of water content. Wet chemical analysis equipment, ratio can be used Weighting or other analytical tools to accurately quantify the water content of such tissues or specimens. When the mannequin is prepared, the water content of the mannequin can be indicated. The water content will be a parameter of the equation. By 125154.doc -15- 200914814 illuminating the boundary of the tissue, mannequin, or specimen with spectra corresponding to different incident wavelengths and measuring by a detector with different source-detector distances gives the ambiguity as a heart) As a result, it can be concluded that the quantity 5 in the equation 4 is recognized, and the 0, 1, 2, 3 'wind 2, 3. In Equation 4, the substitution parameter γ and the amount are also

Calculate the regression parameter %. J As mentioned above, when photons pass through the boundary between different layers of a multi-layered structure, the spectrum of the reflected radiation from the multilayered medium is expected to change significantly due to the different scattering coefficients in the different layers. This change can be used to estimate that the mediator's depth of penetration allows the media to have a strong spectral line for fat tissue. The uneven content in the different layers provides the basis for estimating the number of layers. In addition, the variation and the estimated number of layers can be used to select the detected signal and source detector distance to estimate the content of the analyte, thereby further increasing the accuracy of the analyte content in the multilayer medium. Fig. 3 shows a second configuration of a waveguide fiber in a non-incorporated biological analyte monitor according to the present invention. In the box, in Figure 3, each of the two adjacent fibers in the six concentric configurations is #士Λ, Ρ/+1 is a different source-detector distance of 5 pairs of fiber bundles' which can be special For more layers, the syllabus and the quality k are provided for more detailed information. Preferably, the source-detector distances are equal.

It is understood by those skilled in the art that the two fiber bundle pairs may be discontinuous. Of course, in the real road Temple field Λ μ ^ to use more or less concentric circle configuration. ^ Those who are familiar with this technology should understand that Sun Keliu, the original fiber bundle and detector fiber bundles, was replaced by the father. Refer to Figure 9 for males. .^ +^ Penalty 2 and Figure 3, the source waveguide fiber can be placed in the central £ domain and can be placed around the source φ,, ', and wave fiber configuration waveguide fiber bundle ring to 125154.doc • 16 - 200914814 In this way, a similar measurement can be achieved by adjusting the shadow switch accordingly. In another embodiment of the invention, the radiation source and the detector fiber are respectively emitted and sensed by semiconductor near-infrared (NIR) light. Component replacement. The radiation source can be any one of an LED, a photodiode or a phototransistor. The source-detector distance is defined such that the depth of radiation penetration between the illumination area and the detection area can be adjusted to reach multiple layers. Different layers of the medium. Figure 4 shows a multi-layer analysis flow chart based on spectral measurement. In the processing of the method, in step 1 ,, first, a plurality of light source sources are provided with at least two different The human-shot wavelength electromagnetic light is emitted to illuminate the multi-layer medium. In step 20, the detector is configured to detect the reflection spectrum of the electromagnetic radiation reflected from the multilayer medium (for factory). For each predetermined incident wavelength, In two no The distance of the homologous side is at the distance of the reflectance spectrum. So select the source-detection distance' so that each source, the head, the Wu, the slave, the fourth, the distance, the distance S, the distance S is greater than the number of sources, and the second source The reciprocal of the effective attenuation coefficient of the multi-layer medium corresponding to the incident distance and the incident wavelength, for example, p»l/~. In step 30, the distance between the representation source and the plurality of sources and the human shot are derived according to the equations 等 and Equation 3. The plural of the effective attenuation coefficient corresponding to the wavelength: 'There is then the estimated parameter in the complex quantity 屯 according to Equation 4, and the regression parameter in Equation 4 is determined during calibration using the above calibration method. The data processing in step 3 is implemented. It should be understood that the above embodiments are U-like and not limiting of the invention 'and that those skilled in the art can design alternative embodiments and 125154.doc 200914814 does not deviate from The scope of the attached patent application. In the scope of the patent application, any reference between the springs and the 17th reference should not be construed as limiting the request language "including" does not exclude the ancestors + μ丄, °A element or step that is not listed in the M-item or in the description. A &quot;&&quot; of the ^1 J before the component does not exclude several such components. The present invention A ® is now The praise month can be realized by means of a hardware comprising several different components and by means of a stylized computer. In the system request item enumerating several units, several units in the material unit can be made by the same-item hardware. Or the software. The use of the words -, - - first and second does not indicate any ordering. These words should be interpreted as names. [Simple description of the drawings] By considering the following detailed description in conjunction with the drawings, The above and other objects and features of the invention will become more apparent, in which: Figure 1 is a pictorial representation of a non-invasive biological analyte monitor in accordance with the present invention. Figure 2 is a schematic illustration of a first configuration of a waveguide fiber in a non-invasive biological analyte monitor in accordance with the present invention. Figure 3 is a schematic illustration of a second configuration of a waveguide fiber in a non-invasive biological analyte monitor in accordance with the present invention. Figure 4 is a flow diagram showing an exemplary embodiment of a method in accordance with the present invention. Throughout the drawings, the same reference numerals are used to refer to the same parts. [Main component symbol description] 1 Non-invasive biological analyte monitor 2 Monitor console 125154.doc -18- 200914814 3 Detector 4 Multi-layer medium/skin tissue 4-1 Epidermis 4-2 Dermis 4-3 Subcutaneous tissue 5 Boundary 6 Lamp 7 Optics 8-1 Waveguide Fiber Bundle / Source Fiber Bundle / Source Beam 8-2 Waveguide Fiber Bundle / Source Fiber Bundle / Source Beam 8-3 Waveguide Fiber Bundle / Source Fiber Bundle / Source Beam 8-4 Waveguide Fiber Beam/source fiber bundle/source beam 8-5 Waveguide fiber bundle/source fiber bundle/source beam 8-6 Waveguide fiber bundle/source fiber bundle/source beam 8c excitation end 9 shadow switch 10 waveguide fiber bundle/center detector fiber /Fiber Bundle / Detector Fiber 11 Monochromator 12 Detector Array / Detector Array 13 Electronic Unit 14 Display 15 Data Processing Unit / Data Processing Unit 16 Input Device 17 Medium 125154.doc • 19-

Claims (1)

200914814 X. Patent application scope: 1. A device for non-invasively estimating the content of an analyte in a multilayer medium based on spectral measurement, comprising: a Koda field source for generating electromagnetic waves corresponding to a plurality of incident wavelengths Radiating the spectrum and illuminating the multilayer medium with the spectrum; a detector for detecting a spectrum reflected from the multilayer medium and generating a detection signal indicative of the detected radiation, wherein the radiation is disposed on the multilayer medium An illumination area of the source and a detection area of the detector, to generate a detection signal corresponding to at least two different source-detector distances for each of the plurality of incident wavelengths of the incident wavelength, the source- (4) The device distance is defined as the respective distance between the measured regions of the illumination region (4), and the source-detector distance is much larger than the reciprocal of the effective attenuation coefficient of the multilayer medium; And a data processing device for deriving a plurality of quantities representing the effective attenuation coefficient of the multi-layer medium from the signal of the debt measurement, and using the plurality of quantities to estimate the content of the analyte based on the regression model. 2. The device of the requester ... the source, the definition of the distance of the detector is adjusted so that the depth of penetration of the light penetration between the illuminated area and the area of the ambiguity reaches a different layer of the multilayer medium. The apparatus for claim 3, wherein the data processing device specializes to determine the plurality of quantities: 5 j&gt;\ din[Run J.dPj. Its &quot;W represents one or more incidents Intensity reflection of wavelength λ 125154.doc 200914814 Coefficient 'P; indicates the natural logarithm of a plurality of source·detectors πω, and ^^志—, 1 ω] indicates that the dpj table does not correspond to the source-detector distance# The derivative of _,)], ^) indicates the analyte content corresponding to the distance from the detector. Long and original 4· From: Γ’ The data processing skirt is also used to estimate the content of the analyte from a plurality of quantities according to the following formula: Y^ffa Wherein, representing a calibration-adjustable and determinable regression parameter, the financial value N represents the number of incident wavelengths used for measurement and the number of source-to-indian distances, Λ represents the incident wavelength for reference, and Y represents the analysis. Estimation of the content of the substance. The β of the π-month factor 4 is further prepared by selecting a plurality of incident wavelengths so that the incident wavelengths of the respective β-equivalents correspond to the characteristic lines in the spectrum reflected from the multilayer dielectric. The monthly claim 4 is further prepared, wherein the number of such layers is based on the spectral change from the reflection of the medium, and the number of layers and the spectral change are used to select the source. The detector distance and the detected signal are used to estimate the amount of the analyte. 7. The device of claim 1, wherein the multilayer medium is a living skin tissue&apos; and the analyte is water in the skin tissue. 8. The apparatus of claim 1, wherein the radiation source comprises at least three concentric circle configurations, the equivalent κ configuration respectively having a plurality of waveguide fibers extending substantially to the boundary of the multilayer medium and The boundary 125154.doc 200914814 surrounds the common center detection area, and the detector includes at least one center waveguide fiber. The center waveguide fiber extends substantially to the delta center measurement area on the boundary, and the goods The material processing device is operatively connected. 9. The device of claim 1, wherein the source of radiation comprises a configuration having at least one center waveguide fiber, the center waveguide fiber extending substantially to the multi-layer &quot;quality, and the detector comprising at least three a concentric arrangement having a plurality of waveguide fibers each extending substantially to the boundary of the multilayer dielectric and configured to surround a common central illumination region illuminated by the at least one central waveguide fiber 'And is operatively connected to the data processing device. 10. The device of claim 8, wherein the radiation source further comprises a light source and a switching device coupled to the DH configuration of the waveguide fiber in the radiation source by means of a switching device, the switching device being adapted to selectively Intensify any of the waveguide fiber configurations. 11) As claimed in claim 8 or 9, wherein the source is adjustable to generate an incident spectrum of electromagnetic radiation corresponding to a plurality of predetermined incident wavelengths. 12. The device of claim 8 or 9, wherein the diameter of the core waveguide fiber is selectable such that the diameter is substantially less than the effective attenuation coefficient corresponding to the plurality of incident wavelengths and source-detector distances The reciprocal of each of them. 13_ The device of claim 8 or 9, wherein the radiation source and detector are respectively semiconductor near-infrared (NIR) light emitting and sensing elements. 14. A method for non-invasively estimating an analyte content in a multilayer medium based on spectrometry, comprising the steps of: generating a plurality of different incident wavelengths by using a light source; Electrical 125154.doc 200914814 magnetic Han ray spectrum to illuminate the multilayer medium; detecting a corresponding signal representing the spectrum reflected from the multilayer medium using a detector to be based on two of the plurality of incident wavelengths The source-detector distance is selected to be far greater than the reciprocal of the effective attenuation coefficient of the multi-layer medium; the u-bucket is derived from the detected/received Corresponding to a plurality of incident wavelengths and sources The debt detector distance represents a complex quantity of the effective attenuation coefficient of the multi-layered f; and based on the regression model, the analyte content is estimated based on the complex quantity. 15. The method of claim 14, wherein the definition of the source controller distance is adjustable such that a depth of penetration between the illumination region and the pre-measured region reaches a different layer of the multilayer medium. 16. The method of claim 15, wherein the plurality of quantities are determined according to the following equation: ^〇,7&gt;1 wherein male,) represents an intensity reflection coefficient Ρ corresponding to the - or complex individual wavelength λ; The number of source-detector distances _ 『 and the natural logarithm of the _, and the dp. table does not correspond to the source-detector distance A of 1 peach)] derivative, also indicates The incident wavelength* is the content of the analyte corresponding to the source_detector distance. η. The method of claim 16, wherein the content of the analyte is estimated from the plurality of quantities 125154.doc 200914814 according to the following equation: Y = yfa s{^, Pj) wherein the heart representation is adjustable based on the calibration and The regression parameters of β疋, Μ and Ν respectively represent the number of gongs used in the estimation, and the number of detectors _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Indicates an estimate of the analyte content. Wave 18·-A computer program loaded by a computer device# The summer device includes a method for estimating an analyte device in a multi-layer medium including a processing unit and a memory (1) 基于 based on a first-day test. The η computer program product is capable of performing the following tasks for early processing after the loading. The detection data corresponding to the plurality of incident wavelengths from the multi-layered interface; and the I + output from the detection data , a plurality of incident wavelengths and source-detector distances corresponding to a Λ 曰 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 The content of the substance. 125154.doc