TW200846661A - Chemical indicator test strip - Google Patents

Abstract

Articles and methods for the detection and/or quantification of organic analytes are described. The invention provides a chemical indicator test strip, comprising: a substrate; and a coating comprising a leuco dye complex on the substrate, the coating being insoluble in water and reactive with an organic analyte, the coating derived from a solution of leuco dye and developing agent. In another aspect, the coating further comprises adjuvant in the form of a water-insoluble, polar, hydrophobic, aprotic material that extends the lower limit of detection for the analyte. In still another aspect, the present invention provides a method of using a chemical indicator test strip, comprising: (a) exposing the chemical indicator test strip to a sample comprising an organic analyte; (b) measuring a color change on the chemical indicator test strip following the exposing step; and (c) correlating the color change with the concentration of the analyte in the sample.

Description

200846661 IX. INSTRUCTIONS: [Technical Field] The present invention relates to a chemical indicator test piece and a method for detecting and quantifying an organic analyte using a chemical indicator. [Prior Art] It is desirable to detect and monitor certain organic compounds in various industrial or commercial processes. For example, in many industrial processes such as processes, fermentation and distillation processes in the semiconductor industry, and processes for the extraction and preparation of vitamins, alkaloids, and antibiotics, the detection of solvents in certain process streams is extremely important. In addition, organic pollution from hazardous waste sites and underground storage tanks can jeopardize the quality of underground water, forcing efforts to sample and analyze more and more monitoring wells. In addition, inventory management and quality control factors make it desirable to monitor the concentration of active organic components in ready-to-use formulations prepared (e.g., diluted) from concentrated compositions. Formulations including medium chain length fatty acids and their esters (eg, "toluene fatty acid oxime") are used in the antimicrobial treatment of foods to reduce the amount of foodborne human pathogens in meat and to help protect plants from adverse effects. Effects of fungi and various pathogens that affect the quality and shelf life of fruits and vegetables. However, antimicrobial lipids are generally more expensive than other antimicrobial agents (eg, bleach, peroxides, and/or weak organic acids) and are used in view of cost. It is important to monitor, control, and minimize waste during food disinfection. Current monitoring techniques include the use of gas chromatography and often require long delays between sample collection and analysis results delivery. More generally, conventional monitoring Technology typically utilizes the costly, discontinuous method of introducing uncertainties in sampling and processing procedures. The conventional method does not provide in situ real-time monitoring of the unmet needs of the industry for organic analytes. Substituting 3,31-diphenylpyrene vinegar to produce triphenylcarbenium by interaction with a weak acid component such as bisphenol A Ion. This color-generating reaction was found to be used in microcapsule coating techniques used in carbon-free pressure sensitive carbon paper and thermochromic printing paper. P〇sch & w〇lbeis, Vol. 35, No. 2, No. 89 -94 pages, 1988) The use of thermal paper as a colorimetric indicator for polar solvent vapors in air has been demonstrated. In this application, fully developed strong color thermal paper exhibits a decrease in color intensity after exposure to steam. It is proportional to the vapor partial pressure and this is attributed to the hydrogen bonding network being turbulent by the solvent vapor to convert the color smear dye into the leuco lactone form. Dickert and coworkers (10)/· C/2em· 1988, 60, 1377-1380) A mixture of a lactone dye and a proton donor has been spin coated onto a quartz plate attached to a transmissive optical detection system for use as a photochemical sensor for low boiling polar solvent vapors. The foregoing techniques are limited to the detection of solvents in vapor form. Therefore, it does not provide monitoring of organic compounds in liquid water streams. In addition, such techniques typically require expensive optical and calibration systems, and such techniques are known to be sensitive to both temperature and humidity. The test piece or indicator bar is used to indicate the presence and concentration of the analyte in the liquid by immersing the piece or indicator rod in a liquid and observing the color change and color intensity. These products include common litmus test for pH measurement. And complex tests for detecting clinically significant markers in biological fluids, such as glucose or protein in blood and urine samples. Test strips or indicator sticks are inexpensive disposable analytical tools that are highly accurate and visually comparable Instrument chart or I27765.doc 200846661 is used qualitatively in conjunction with readout devices for quantitative information and electronic record keeping. Therefore, it is desirable to provide test strips that can be immersed in water and used to quantitatively measure and monitor water-miscible organic compounds or Indicative Sticks. SUMMARY OF THE INVENTION The present invention provides articles and methods for detecting and/or quantifying organic analytes. In the aspect of the invention, the invention provides a chemical indicator test sheet comprising: a substrate; and a coating comprising a leuco dye on the substrate, the coating being insoluble in water but reacting with an organic analyte, the coating (d) A solution derived from a leuco dye and a developer. The terms used herein are to be understood to have the same meaning as those given by those of ordinary skill in the art. For clarity, certain terms are given below. Further definitions. As used herein, "hidden dye" means that the molecule is available in two forms, one of which is a colorless dye. "leuco dye complex" means a complex composed of one or more leuco dyes in combination with one or more developers. Abbreviation for oxime methyl isobutyl. In another example, the present invention provides the foregoing chemical indicator test sheet, wherein the 4-coating layer comprises an adjuvant, wherein the adjuvant is a water-insoluble, polar, hydrophobic, aprotic material and can be used for the analyte. (4) The limit is expanded to be lower than the detection limit available when the adjuvant is not present. The present invention provides a method of using a chemical indicator test piece 127765.doc 200846661, the method comprising: (a) exposing a chemical indicator test piece to a liquid sample comprising an organic analyte stored in a solution, The chemical indicator test piece comprises: a substrate; and a coating comprising a leuco dye complex on the substrate, the coating is not combined with water but reacted with an organic analyte, the coating being derived from a leuco dye and a solution of the developer; (b) a color change on the chemical indicator test sheet after the exposure step is detected; (c) correlating the change in color with the concentration of the analyte in the sample. Those skilled in the art will be able to more fully appreciate the features of the present invention after considering the present disclosure, together with the various examples and figures, and the accompanying claims. [Embodiment] Hereinafter, embodiments of the invention are explained. The present invention provides chemical indicator test strips and methods for qualitatively and quantitatively monitoring organic compounds using chemical indicator test strips. Embodiments of the present invention provide colorimetric, immersible chemical indicator tests that can be used to quickly and quantitatively determine the concentration of organic compounds in water or other solvents.

sheet. As used herein, "chemical indicator test strips" "" test strips are used interchangeably when referring to articles of the invention. X The test strips of the invention comprise a leuco dye complex coated and in a solvent and especially aqueous A substrate capable of undergoing impregnation in a system. In the present invention; the leuco dye complex is initially formulated with a liquid indicator composition comprising a leuco dye, a developer, and optional other components that enhance the performance of the test strip. The form is provided. In some embodiments, 'additional optional other components are added to improve the ability of the final chemical I27765.doc 200846661 indicator test strip to detect a particular analyte and/or to improve the sensitivity of the test strip at a lower analyte concentration 4 The body indicator composition is applied to the substrate and dried to provide the chemical indicator test piece of the present invention. The leuco dye complex comprises at least one coupler and at least one imparts a non-white or colored appearance to the chemical indicator when combined The sizing agent on the surface of the substrate of the test piece. However, the sigma dye complex is recovered or "bleached" to the presence of an organic analyte capable of solvating the developer. State. The color change or degree of bleaching is directly proportional to the concentration of the analyte in the water, so as the concentration of the organic analyte increases, the color of the chemical indicator test piece becomes lighter. According to the previous calibration, the extent to which the test piece changes color can be correlated to the analyte concentration. The leuco dye The leuco dye complex used in the chemical indicator test sheet of the present invention comprises a coupler and a developer. In the examples of the present invention, suitable types of leuco dyes include fluorescent yellow precursors, rhodamines, and triarylmetholone dyes. The specific compound is reacted with an acidic developer (e.g., Lewis acid, salicylic acid, phenolic compound, or acid clay) to form a highly colored material. Specific examples of such compounds include, but are not limited to, those under the trade name "PERGASCRIPT" (Ciba

Specialty Chemicals of Tarrytown, New York). Another leuco dye used in the examples of the present invention is the leuco dye crystal violet lactone (triarylmethane lactone). In its lactone form, the crystal violet lactone is colorless or pale yellow. However, in a low pH environment, it is protonated and exhibits a strong purple color. The following are suitable for the leuco color used herein alone or in combination of two or more. 127765.doc 200846661 Non-limiting list of dyes: fluorenyl golden amine thiochrome 嗟 秦 秦 (X-unsaturated aryl ketone Azaindole* Benzopyridyl leuco indigo • Benzoyl leucoxazine Benzyl fluorenyl thiazine 10 β-unsaturated aryl ketone basic monoazo dye bis-indole Azlactone 10-Benzenyl-Team Team>1-Tetraethyl-3,7-diamino-1011-morphoxazine sigma 17 sitting blue chromogenic azaindole compound crystal violet Ester diaryl S 酉 酉 ^ ^ Diphenylmethane dithio-glucamine, bis [bis-(indenyl) extended ethyl] tetrahalodecanolide, fluorescent yellow matrix fluorescent yellow matrix derived (for example, 3-dialkylamino-7-dialkylpentylfluorescent yellow mother) Green vinegar 3-(indol-3-yl)-3-(4-substituted aminophenyl) Azlactone 127765.doc -10- 200846661 fluorenyl bis-(fluorenyl) ethylene ruthenium leuco-l-amine leuco benzyl phthalocyanine blue leuco malachite green '3-methyl-2, 2-spirobis(benzo-[f]-pinene)- phenoxazine azlactone leuco dye 10 Phthlan poly(phenylene sterol) and 8 » decyl benzo-n-bambolin and snail-like rhodamine β-indoleamine sulphate-substituted 4,7-diazepine lactone Sultines Michler's hydrol p-toluene folate φ triaryl carbaryl triphenyl decane (gentian violet and malachite green) 3,3-one base-3H-2,1 - the present oxosulfate 1 _ oxide developer developer is incorporated into the leuco dye complex of the present invention. These developers pass through % as an electronic genre, but more accurately should be described as a proton donor. In the embodiment, the developer is insoluble in water. In certain embodiments, the indicator 3 is selected from the group consisting of the following weak acids: p-hydroxybenzoic acid octyl vinegar, methyl p-hydroxybenzoate, 1,2,3- Combination of oxadiazole, 4-hydroxycoumarin derivative and two or more of the former 127765.doc -11 - 200846661. In certain embodiments, it can be used in the leuco dye complexes described herein. Louise acid "Tanning agent. These examples include active clay materials such as attapulgite, acid clay, bentonite, montmorillonite, acid-activated bentonite or montmorillonite, Halloysite (haii〇 "ite), stone dioxide Xi, Shao oxide, aluminum sulfate, phosphate, Ming, hydrated hammer dioxide, zinc chloride, zinc term, activated kaolin or other clays.

In other embodiments, an acidic organic compound is used as the developer. Examples of such developers include cyclo-substituted phenols, resorcinol, salicylic acid (e.g., '3,5-bis(with _dimethylmethyl)salicylic acid; 3,5-bis((γ) Methyl group) salicylic acid) or salicylic acid vinegar and its metal salt (for example, zinc salt) additional acidic organic compounds including certain polymeric materials such as phenolic polymers, alkylphenol acetylene resins, maleic acid / Partial or complete hydrolysis of rosin resin or maleic anhydride with styrene, ethylene or vinyl methyl ether or carboxymethylene. Mixtures of two or more monomers and polymeric acidic organic compounds may also be used. In other embodiments, the developer may be selected from phenolic resins or phenolic compounds such as 4-tert-butylphenol, 4-phenylphenol, methylenebis(p-phenylphenol), 4- Hydroxydiphenyl ether, α-naphthol, naphthol, methyl benzoyl benzoate, benzyl 4-hydroxybenzoate, 4-hydroxydiphenyl sulfone, hydroxy acetophenone, 2, 2'-one Hydroxydiphenyl, 4,4'-cyclohexylene phenol, 4,4 isopropylidene diphenol, 4,4-isopropylidene bis(2-methylphenol), zinc thiocyanate Acridine complex, 4,4-bis(4-hydroxyphenyl)pentanoic acid, hydroquinone, resorcinol, m-diphenol, p-hydroxybenzoic acid, m-hydroxybenzoic acid, o- Hydroxyl 127765.doc • 12- 200846661 base benzoic acid, gallic acid, 1-Phenyl-2-naphthoic acid. In still other embodiments, the developer is poly(4-vinyl benzene). In an embodiment, the developer is ruthenium, (9-fluorenylene), diphenyl phthalate. auxiliaries In certain embodiments of the invention, the leuco dye complex is optionally at least water-incompatible. , polar, hydrophobic, #proton, component form of adjuvant formulation: in the context of illustrating the adjuvant, "polar" refers to the ability of a material to form hydrogen bonds. In some embodiments, the polarity, The hydrophobic, aprotic adjuvant has a low vapor pressure (e.g., 1 mm Hg). An adjuvant (when present) is added to the initial liquid indicator composition along with the leuco dye complex. The liquid indicator can then be used The composition is applied to the substrate for drying to provide a chemically acceptable beta tablet having an improved analytical range. § t 'The presence of adjuvants improves the sensitivity of chemical indicators at lower analyte concentrations, increasing the lower limit of detection for specific organic analytes. At lower concentrations, it is desirable to detect specific analytes, usually more It is desirable to include an adjuvant in the chemical indicator test strip of the present invention because the detection limit for the organic analyte in the presence of the adjuvant is much lower than the detection limit that would otherwise be available in the absence of the adjuvant. In certain embodiments The inclusion of the adjuvant allows the analytical range of the analyte in the water to be extended down to about 0.2% by weight. The adjuvant compound is typically selected from compounds which do not impart color to the leuco dye. Representative and suitable compounds derived from the species include Aliphatic esters of carboxylic acids and aromatic vinegars, for example, those selected from the group consisting of citrates, phthalates, adipates, benzoates, sebacates and benzoates. In certain embodiments of 127765.doc -13.200846661, the adjuvant is selected from the group consisting of organophosphates, organic sulfonates, and sulfonamides. In other embodiments, an adjuvant having a large amount (e.g., greater than 3) of polar functional groups per molecule is preferred. In other embodiments, the adjuvant is selected from the group consisting of citrates and sulfonamides. Surfactants In various embodiments of the invention, the chemical indicator test strips of the present invention comprise a coating having a leuco dye complex in combination with a surfactant. In such embodiments, one or more surfactants are included in the initial liquid indicator composition applied to the substrate. After drying, the resulting coating includes a leuco dye complex and a surfactant. One or more surfactants can be incorporated with the dye complex to help wet the substrate in an aqueous environment and aid in transporting the organic analyte into the chemical indicator test strip and combining with the leuco dye complex thereon in. Suitable surfactants may be selected from compounds which do not impart color in combination with leuco dyes. As will be further explained below, the surfactant can be incorporated into the coatable liquid indicator composition to be applied to the substrate, in combination with the leuco dye. Human surfactants may be desired for certain organic analytes. For example, σ, the detection, monitoring and/or quantification of the antibacterial fatty acid monoester in the water is high by the presence of a surfactant in the chemical indicator test piece of the present invention. These antibacterial agents can be used, for example, in the antibacterial treatment of foods to prevent contamination by a wide range of protoplasts. In commercial applications, the fatty acid monoacetate is provided in a concentrated composition of between about 1. (4) and 0% by weight of the monoester: the user. However, 'before the monoester is applied to the food, the concentrate can be diluted with water to provide 罝砰, 杈 for early days, and the degree is between about (^(^ and 5·〇% by weight and at 127765.doc • 14-200846661 In some embodiments between about 0.005 and 1.0% by weight of a diluted and ready-to-use fatty acid monoester formulation. In embodiments of the invention, one or more surfactants may be incorporated into the substrate onto the substrate. A liquid indicator composition in a liquid indicator composition. The coating method of the liquid composition and related formulations are additionally described below. In the embodiment in which the surfactant is incorporated, it may not be included in addition to the surfactant. Auxiliaries. For example, the use of the chemical indicator test strips of the present invention for the detection of certain fatty acid esters (eg, c8i C 2 fatty acid esters) (eg, for use in food antibacterial treatment) can generally be included without adjuvants. The following is carried out to detect vinegar in a ready-to-use formulation within the range of ester concentrations described herein. The present invention provides a method for the detection and quantification of fatty acid monoesters using the chemical indicator test pieces described herein. The chemical indicator test piece can detect a fatty acid monoester including one or more fatty acid esters of a polyhydric alcohol, a fatty ether of a polyhydric alcohol, an (ester or ether) alkoxylated derivative thereof, and a combination of two or more of the former. Such compositions include an antimicrobial component comprising a fatty alcohol ester of a hydroxy acid, an alkoxylated derivative thereof, or a combination thereof. An effective amount of the antimicrobial component (typically, an antimicrobial lipid component) comprises (CrC8) hydroxycarboxylate. Acid (cvc) 4) a saturated fatty alcohol monoester, (CyC8) hydroxycarboxylic acid ((^_匕9 mono- or poly-unsaturated fatty alcohol, one of the alkoxylated derivatives of the former or Combination wherein the alkoxylated derivative has less than 5 moles of alkoxylate/mole hydroxycarboxylic acid. Suitable surfactants may be selected from any of the four major groups, anionic, cationic, nonionic And zwitterionic (amphoteric) surfactants. Illustrative surfactants useful in the present invention include those set forth in Examples 127765.doc -15 - 200846661 herein.

Exemplary cationic surfactants include, but are not limited to, salts of a primary, secondary or tertiary fatty amine alkylated by polyoxyalkylene, as appropriate; quaternary ammonium salts such as tetraalkylammonium, alkylguanidino alkane a trialkylammonium, a trialkyl benzyl group, a trialkyl hydroxyalkyl ammonium, or a compatible anion counterion (eg, a cerium ion (preferably chloride or bromide) or an alkyl sulphate, such as a methyl group Alkyl pyridine rust of sulfate or ethyl sulphate and other anionic counterions; odorant derivatives; amine oxides having cationic properties (for example at acidic pH). In certain preferred embodiments, the (etc.) surfactant comprises one or more cationic surfactants selected from the group consisting of tetraalkylammonium, trialkylbenzylammonium, and alkylpyridinium halides. And mixtures thereof. In certain embodiments, amine oxide surfactants may be employed, such as those including alkyl and alkyl guanylalkyl dialkyl amine oxides. Examples of amine oxide surfactants include those under the trade names AMMONYX LO, LM LMDO and ΑΜΜΟΝΥΧ CO (which are lauryl dimethyl amine oxide, lauryl guanidinopropyl dimethyl amine oxide and cetyl oxide). Amines are available from Stepan. In other embodiments, anionic surfactants may be used including, but not limited to, sarcosinates, glutamates, alkyl sulfates, alkyleth sulfates or potassium, alkyls Alcohol polyether ammonium sulfate, laureth ammonium orthosulfate, lauryl polyether orthosulfate, isethionate, alkyl and aryl ether glyceryl ether sulfonate, alkyl and aryl a sulfo-succinyl I salt (eg, 'dioctylsulfosuccinate), an alkyl glyceryl ether sulfonate, a decyl % fee salt, an aryl phosphate, an alkyl phosphonate, and an aryl alkyl phosphonic acid 127765.doc -16 - 200846661 Salt. The anionic surfactants can have metal or organoammonium counterions. In certain embodiments, the anionic surfactant used in the compositions of the present invention is selected from the group consisting of sulfonates, sulfates, phosphates, phosphonates, and combinations of two or more of the former. . Suitable sulfonates and sulfates include alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, alkyl ether sulfonates, alkyl benzene sulfonates, alkyl phenyl ether sulfates, alkyl sulfo groups Acetate, secondary alkane sulfonate, secondary alkyl sulfate, and the like. Commercially available alkyl ether sulfonates (e.g., lauryl ether sulfate) are commercially available from Stepan under the trade names POLYSTEP B12 and POLYSTEP B22. Other commercially available anionic surfactants include sodium decyl tauronate (available under the trade name NIKKOLCMT 30 from Nikko Chemicals, Inc., Tokyo, Japan); secondary pyrocarbonate, for example, available from Clariant (Charlotte, NC). Hostapur SAS (the system (C14-Ci7) secondary burned hydrocarbon sodium sulphate (α-olefin sulfonate)); alkyl sulfonate-2-sulfonate, for example, available under the trade name ALPHASTEP PC-48 from Stepan The company's mercapto-2-sulfonic acid (Ci2-16) S is the second and 2-acid acid (Ci2_Ci6) fatty acid di-nano; calcined acetate and alkyl sulfosuccinate, can be used as a lauryl Sodium sulfoacetate (under the trade name LANTHANOL LAL) and sodium laureth sulfosuccinate (STEPANMILD SL3), both available from Stepan; alkyl sulfates, for example, available under the trade name STEPANOL AM Stepan Corporation's ammonium lauryl sulfate; dialkyl sulfosuccinate, for example, as AEROSOL OT, purchased from Cytec Industries, dioctyl sulphate. Suitable phosphates and phosphonates include alkyl phosphates, alkyl ether phosphates, 127765.doc -17- 200846661 aralkyl phosphates and aralkyl ether phosphates. Such surfactants may include mono-, di-, and tri-(alkyltetra-glycol ethers)-commonly known as trilauryl poly-4-phosphate and available from Clariant under the trade name HOSTAPHAT 340KL. a mixture of phosphates, and PPG-5 ceteth 10 phosphates and mixtures thereof available under the tradename CRODAPHOS SG from Croda Corporation (Parsipanny, NJ). In other embodiments, an amphoteric surfactant can be used and includes a surfactant having a protonatable tertiary amine group and a quaternary amine containing amphoteric surfactant. Useful amphoteric surfactants include, but are not limited to, carboxylic acids

I am an amphoteric surfactant and a continuous acid amphoteric surfactant. Examples of tarenic amphoteric surfactants include, but are not limited to, certain betaines such as coconut betaine and cocoamidopropyl betaine (available under the tradename MACKAM CB-35 and MACKAM L from McIntyre Group). Ltd., University Park, 111.); monoacetate, such as sodium lauryl acetate; diacetate, such as disodium lauryl amphoacetate; amine- and alkyl-amino-propionate, such as laurylamine Propionic acid (commercially available under the trade names MACKAM 1L, MACKAM 2L and MACKAM 151 L from McIntyre Group Ltd.). Examples of ammonium sulphonate commonly referred to as "sultaine or sulfobetaine" include cocoamidopropyl via sylcholine yellow beet test (MACKAM 50-SB from McIntyre Group Ltd.) ). These contiguous amphoteric surfactants are preferred over the slow acid salt amphoteric surfactants because the sulfonate groups will remain ionized at lower pH values. In still other embodiments, nonionic surfactants can be used including, but not limited to, alkyl glucosides, alkyl polyglucosides, polyhydroxy fatty acids 127765. doc -18 - 200846661 decylamine, sucrose esters, Esters of fatty acids and polyols, fatty acid alkanolamines, ethoxylated fatty acids, ethoxylated aliphatic acids, ethoxylated fatty alcohols (for example, sulphate available under the trade name TRITON X-100) Phenoxy ethoxypolyethoxyethanol and mercaptophenoxy poly(ethyleneoxy)ethanol available under the trade name NONIDET P-40, both available from Sigrna, St. Louis, Mo.) Ethoxylated and/or propoxylated aliphatic alcohols (for example, available from ICI under the trade name BRIJ), ethoxylated glycerides, ethoxylated/propoxylated block copolymers (eg, PLURONIC and TETRONIC surfactants from BASF), ethoxylated cyclic ether adducts, ethoxylated guanamine and imidazoline adducts, ethoxylated amine adducts, An ethoxylated thiol adduct, an ethoxylated condensate with an alkyl phenol, and a nitrogen-based ethoxylate , ethoxylated polypropylene oxide, polymeric polyoxymethylene, fluorinated surfactants (for example, they are available under the tradename FLUORAD-FS 300 from Minnesota Mining and Manufacturing Co., St. Paul, Minn. And may be traded under the trade name ZONYL from Dupont de Nemours, Wilmington, Del.), and polymerizable (reactive) surfactants (for example, available under the trade name MAZON from PPG Industries, Pittsburgh, Pa. SAM 211 (alkyl polyalkoxy sulfate) surfactant). In certain embodiments, the nonionic surfactants useful in the compositions of the present invention are selected from the group consisting of Poloxamer (e.g., PLURONIC from BASF), sorbitol. Anhydride fatty acid esters and mixtures thereof. It is to be understood that the aforementioned surfactants may be used singly or in combination of two or more of the former. 127765.doc • 19- 200846661 Substrate As described above, the aforementioned dye complex was applied to a substrate to provide an indicator test piece of the present invention. Any of a variety of materials can be used as the substrate or substrate material to which the dye complex is applied. In certain embodiments, the substrate material can include cellulosic and non-cellulosic materials such as, for example, paper, natural or synthetic fibers, from, for example, cotton, rayon, hemp, jute, bamboo fiber, acetate fiber Lines and yarns made of materials such as carboxymethylated solvent-spun cellulose fibers and the like. In certain embodiments, the substrate can comprise a material selected from the group consisting of polyester, polyamide, polypropylene decylamine, polyacetate, polyacrylic acid, polyolefin (eg, water propylene, polyethylene, ethylene propylene) Copolymer and ethylene butene copolymer), polyurethane (including polyurethane foam), ethylene (such as polyvinyl chloride), polystyrene, fiberglass, ceramic fiber, glass, two Oxygen cut, polyacrylic acid vinegar, polyacrylonitrile, polydifluoroethylene, polytetrafluoroethylene, polyoxymethylene, polyvinyl alcohol, polylactic acid, polyvinyl ether 'polyethylene ketone, polycarbonate, A styrene-ethylene butylene-styrene elastomer, a styrene-styrene-styrene elastomer, a stupid ethylene isoamyl elastomer, and a combination of two or more of the former. In some embodiments, the combination of materials can be incorporated into the substrate, and two or == any combination of the foregoing materials should be within the range of acceptable substrates for use in the chemical indicator salt tablets of the present invention. In various embodiments, the substrate can be coated onto or immersed in, for example, a porous or non-porous complex. In the present invention; 127765.doc -20- 200846661, the substrate can be bent, w "A* IU' stone, woven or non-woven material. In certain embodiments, the substrate can be a polymeric web (non-woven or woven), a polymeric film, a hydrocolloid, a foamed metal foil, paper, and/or a combination of two or more of the former. In some such embodiments, the substrate can be, for example, absorbent cotton gauze or cotton swab. In other, expanded embodiments, the substrate is, for example, glass, such as a glass slide. Suitable porous materials for use as substrates in the embodiments of the present invention include paper, fabrics, woven fabrics (such as cheesecloth and gauze), non-woven fabrics (including spunbonded and BMF (blown microfibers)), and extruded porous sheets. And the hole piece. Preparation of Chemical Indicator Test Sheet In preparing the chemical indicator test piece of the present invention, the liquid indicator electric person is applied to a suitable substrate and dried. The preparation of the test indicator test sheet of the present invention includes preparing a coatable liquid indicator composition which can be applied to the substrate. And a substance (10), the liquid indicator composition is prepared by adding at least one leuco dye and at least one hydrazine to hydrazine. The combination of the agent composition and the active agent. "He μ includes a mixture of auxiliary agents and/or surface-coatable liquid indicator compositions, and gentleman's and θ-cuts are added to the tablets in either order to ensure the components. Dissolved in a solvent. In some embodiments, the smear, the y, the y, and the smear

Auxiliaries in a suitable solvent (for example, M heat _ ^ redundancy or methyl isobutyl ketone). The concentrations of the various components are also varied within the scope of the invention. In the case of staying 2, the singularity is about 2 " the degree is between about 0.02% by weight and the force of 2.0%, and the range is between yoke and yoke. The leuco dye 127765.doc -21 . 200846661 is within a range between about 〇·〇5% and about L〇% by weight. In still other embodiments, the concentration of the leuco dye is in a range between about %1% and about 〇·3 wt%. As mentioned above, the liquid indicator composition comprises a developer. In certain embodiments, the concentration of the developer is within a dry circumference of between about G2% and about 2G wt%. In other embodiments, the developer is between about 5% and about 10% by weight. /. Within the range. In still other embodiments, the concentration of the developer is in a range between about 1% and about 3% by weight. In the case of the fishing κ, the weight ratio between the leuco dye and the developer may be between about 10/1 and about 1/1 () (). In certain embodiments, the weight ratio between the leuco dye and the developer can be between about 5/1 and about 1/2 (). In still other embodiments, the weight ratio between the leuco dye and the developer can be between about 1/1 and about 1/10. In embodiments wherein the liquid indicator composition comprises an adjuvant, the concentration of the adjuvant is in a range between about 0.2% and about 2% by weight. In other embodiments, the adjuvant is in a range between about 5% and about 5% by weight. In still other embodiments, the concentration of the adjuvant is in a range between about 1% and about 3% by weight. In such embodiments, the weight ratio between the leuco dye and the adjuvant may range from about 1 〇/1 to about 1/100. In certain embodiments, the weight ratio between the leuco dye and the adjuvant can be between about 5/1 and about 1/2 G. In still other embodiments, the weight ratio between the leuco dye and the adjuvant can be between about 1 and about 10/10. In embodiments wherein the liquid indicator composition comprises a surfactant, 127765.doc -22- 200846661 the concentration of the surfactant is in the range of between about 0.04% and about 4% by weight. In other embodiments, the surfactant is in a range between about 0.08 ° / Torr and about 2 % by weight. In still other embodiments, the concentration of the 5H surfactant is within a range of between about 0.2% and about 0.6% by weight. In such embodiments, the weight 1 ratio between the leuco dye and the surfactant may range from about 5/1 to about 1/5. In certain embodiments, the weight ratio between the fe, color dye and surfactant can range from about 3/1 to about 1/3. In still other embodiments, the weight ratio between the leuco dye and the surfactant can be between about 2/1 and about 1/2. In certain embodiments, the process used to prepare the chemical indicator will include the selection of the substrate. In some embodiments, the available surface area and adsorption characteristics of the material can be a factor in selecting a suitable substrate. By way of example, the porous material can provide a substrate having more surface area on which the indicator composition is applied. The surface area and adsorption characteristics of the substrate are also a factor in how easily the organic analyte is introduced to solvate with the dry leuco dye complex. In certain embodiments, the non-absorbent material can be more readily retained on the outermost surface of the non-absorbent substrate than the absorbative dye. In ==, when the chemical indicator test strip of the present invention is used to monitor a particular aqueous environment, the dried dye complex can more readily form hydrogen bonds with the organic analyte. In such embodiments, a non-absorbent material having at least some porosity is preferably used as the substrate to increase the total surface area of the substrate used to coat the leuco dye complex. The process of applying the liquid indicator composition to the substrate requires depositing a sufficient amount or volume of the liquid indicator composition to wet at least the appropriate amount or volume of the substrate - Table 127765.doc -23- 200846661 / ΘΤ. Any amount or body filament coated by the dye-coated surface for the sub-volume of the temple is usually 'printed by the dye surface: the area of the foot: to allow representative sampling of the aqueous system or the like: additionally 'expected coated substrate The surface area should be of a sufficiently large area to allow the change in color to be readily detected by electrons (e.g., densitometer or such as measured. In some embodiments, 'providing a dye complex to the substrate: tightly coupled Final article, light or thin coating of the indicator composition: preferably. The actual wet coating weight of the liquid indicator composition applied to the substrate can be viewed as a dye indicator and other selected to be included in the final article. The composition, the material selected for the substrate, the coatable surface area of the selected substrate, and the like, vary, and the appropriate coating weight of the indicator composition on the substrate is It is desirable to experiment within the skill of those skilled in the art. After drying, the liquid indicator composition forms a colored coating on the substrate and the resulting article can be used as a chemical indicator test piece of the present invention. By any suitable method, in some embodiments, drying is by placing the wetted substrate in a furnace or the like at elevated temperature for a time sufficient to drive off the solvent and provide a dried article suitable for use as described herein. In some shell yoke examples, the wetted substrate is dried by simply evaporating the solvent at ambient or room temperature. In other embodiments, the wetted substrate can be at a high temperature (eg, 15 ( TF or 66 it is placed in the oven for a short period of time (eg, a few minutes). After drying, the resulting article is used as a chemical indicator test strip. Those skilled in the art will understand that the chemistry used to make the invention The method of the indicator test piece is further illustrated in the various examples herein. 127765.doc • 24· 200846661 Use of the chemical indicator test piece The chemical indicator test piece of the present invention provides a non-specificity Tools to detect the presence of organic analytes in a solvent such as water. In processes or systems in which the analytes are known, the chemical indicator test strips of the present invention provide a convenient and rapid method for qualitative and/or quantitative monitoring of organic analytes. The chemical indicator test strips of the present invention can be used to monitor any of a plurality of processes to detect and/or measure the amount of any of the various organic analytes in an aqueous formulation, process stream, bath, or the like.

In the case where the chemical indicator test piece is only used to qualitatively monitor the presence or absence of the analyte, the chemical indicator 简单m piece is simply immersed in a process stream or other liquid in which the analyte may be present. The indicator test strips can be immersed in the liquid sample and warmed (iv) for a sufficient period of time to adequately thirst the sheet (e.g., 'ig-2g seconds). After the test piece is taken out of the liquid, it can be read with a paper towel or the like to remove excess liquid. In some embodiments, the presence of the analyte can be visually detected by observing the color change on the test piece, typically by observing the color of the dye complex on the substrate. If required, the tablet can be compared to a "control" tablet with the initial or starting color of the dye complex immersed and exposed to the organic analyte. 2=Some implementations, by "electronic comparison test" The immersion in the test = and then its color to determine whether a color change occurs makes it easier to understand the qualitative flaws of the presence of organic analytes. For example, ^ leaf by measurement! The optical density of the exposed test piece relative to the unexposed test piece: used to determine the color change. % In some embodiments _, Tai Gong _ Ben Ming chemical and indicator test strips are used to qualitatively determine 127765.doc -25- 200846661 to determine the presence of organic analytes. In the quantitative and quantitative determination of the organic analytes, the concentration of the eucalyptus, the standard curve is prepared to provide a relationship between the photon mountain and the knife-out; This standard curve can be based on a set of expected chemistry similar to that of the group, which is similar to the intended composition, 隹, 々, 机, machine, or combination. Usually, the titanium quasi-analyte solution is prepared to cover the range of the expected monitoring process of the person, and the range of the extract. The above standard can be used, and then the optical density and the score can be established. Then, root

According to the test of the light of the test film, the optical density readings on the samples obtained during the monitoring are compared with the % drought curve to determine the concentration of the analyte in the sample. In the quantitative determination of organic knife extracts, the standard composition used to determine the standard curve is preferably similar to or substantially chemically identical to the composition of the intended sample. In the use of chemical indicator test strips for this quantitative determination, the amount of time for any of the blunt patches to flow through the cleavage process stream or the like should be substantially the same between samples and should be the same for a standard solution. In the presently described embodiments, the present invention provides an organic analyte in a composition, process stream or the like having an analyte length between about 〇% and about 5% by weight. . In certain embodiments, certain organic analytes have a detection limit of between about 〇% and about 25% by weight. EXAMPLES Those skilled in the art will be able to clarify other aspects of the use of the chemical indicator test strips of the present invention after considering various non-limiting examples herein. Example 1 (Using a substantially water-insoluble scorpion dye complex construct indicator sheet) 127765.doc 26· 200846661 20 mg of Pergascript Blue URN from Ciba-Geigy Specialty Chemicals, NC was dissolved in 5 grams from Aldrich Chemicals Methyl isobutyl ketone (MIBK). 200 mg of poly(4-vinylphenol), M.W. = 8000 Da as a developer and 20 mg of sodium dioctylsulfosuccinate surfactant (both from Aldrich Chemicals) were dissolved in 5 g of MIBK, respectively. The two solutions were mixed together to give a clear, homogeneous solution. Blank indicator tablets were thermally bonded to 4"χ0·3" polyester sheets by using a supported double-sided PSA to transfer Whatman 114 filter paper (from Whatman International, England) 0.3 "χ0·3" Prepared on one end of mil thick. Use 5 μl of solution to spot on paper. The initial colorless spots turned dark blue after evaporation of MIBK. The chemical indicator test piece was dried at 150 °F for 1 minute and cooled. (Self-dye complex and water-soluble developer-bisphenol-A construction indicator tablet) 20 mg of Pergascript Blue I-2RN from Ciba-Geigy Specialty Chemicals, NC was dissolved in 5 g of methyl obtained from Aldrich Chemicals In isobutyl ketone (MIBK), 200 mg of bisphenol-A and 20 mg of dioctyl sulphate sodium surfactant (both from Aldrich Chemicals) were dissolved in 5 g of MIBK, respectively. The two solutions were mixed together to give a clear, homogeneous solution. The blank chemical indicator test strips were heated by a 0.33" χ0·3 π sample of Whatman 114 filter paper (from Whatman International, England) using a supported double-sided PSA. Bond to 4"χ0·3" Ester was prepared sheet (5 mil thick) end. A 5 microliter solution was used to spot on the paper. The colorless spots at the beginning of the MIBK evaporation turned dark blue. The indicator sheet was dried at 150 °F for 1 minute and cooled. 127765.doc -27· 200846661 Example 2 (Use of indicator in Example 1) Preparation of n-propanol with a range of 〇% w/w to 14% w/w (from

Aldneh Chemicals) aqueous solution. The chemistry of the type described in Example 1 was prepared and/or the tablets were immersed and each was immersed in the solutions for 15 seconds under gentle agitation. Each piece was then removed from the solution and quickly blotted dry onto the absorbent paper towel using a gentle pressure' and the optical density was measured immediately on a MacBeth RD917 densitometer using a green filter. It can be visually discerned that the color intensity gradually decreases as the concentration of propanol increases. The graphical representation of the results is shown in Figure 1, which illustrates that the optical density of the indicator is linear with respect to the aqueous concentration of the indicator greater than about 6%. Example 3 (Indicator in Example 1 for low vapor pressure and high vapor pressure solvent) Acetone and propanezepine carbonate were prepared at concentrations ranging from 0% w/w/s 14% w/w (one from Aldrich) Chemicals) aqueous solution. Chemical indicator test pieces of the type described in Example i were prepared and each was sub-crushed in the solutions for 15 seconds under gentle agitation. Each piece was then removed from the solution, quickly blotted dry onto the absorbent paper towel using a gentle pressure, and the optical density was measured immediately on a MacBeth RD917 densitometer using a green filter setting.彳 Visually discerning the color intensity gradually decreases as the solvent concentration increases. The results are not shown in Figure 2, which shows that even though the propylene carbonate has a vapor pressure much lower than that of acetone (185 mil Hg at 20 ° C), 〇·〇 3 mm Ug at 20 C, but each The change in unit concentration shows a higher optical density change. The linear range of optical density versus concentration extends down to 4% compared to the lower limit of 6% of acetone. Example 4 (Use of different lactone dye construction indicators and their use) 127765.doc -28- 200846661 20 gram of Pergascript Red I_6B from Ciba-Geigy Specialty Chemicals, NC was dissolved in 2.5 grams of MIBK. 200 mg of poly(4-vinylphenol), M.W. = 8000 Da and 20 mg of sodium dioctylsulfosuccinate were bathed in 2 · 5 g of MIBK. The two solutions were mixed together to give a clear homogeneous solution. Blank indicator film will be used by using supported double-sided pSA

Whatman 114 crepe paper (from Whatman Internati〇nal, England) 2 〇·3”χ〇·3" The sample was thermally bonded to one end of a 4, χ〇·3” polyester sheet (5 mil thick). A 5 microliter solution was used to spot on the paper. The initial colorless spots turned dark red after the MIBK evaporates. The indicator piece was dried under 丨5 1 for 1 minute and cooled before use. An aqueous solution of n-propanol having a concentration ranging from 0% w/w/ to 16% w/w was prepared. Chemical indicator test pieces of the above type were prepared and each immersed in the solutions for 15 seconds under gentle agitation. Each piece was then removed from the solution, quickly blotted dry onto the absorbent paper towel using a gentle pressure, and the optical density was measured immediately on a MacBeth RD917 densitometer. It can be visually recognized that the color intensity gradually decreases as the solvent concentration increases. The graphical representation of the results is shown in Figure 3 below. As with the chemical indicator test piece including the blue dye (see Fig. 1), the chemical indicator test piece prepared using the red dye also exhibits a linear region in which the optical density changes with concentration. However, the blue indicator exhibited a lower linear detection limit (6%) than the red indicator (10%). Example 5 (using 4,4'-(9-fluorenylene)-diphenol as a hydrophobic developer construction indicator and its use) 20 mg of leuco dye Pergascript Blue I-2RN (Ciba-Geigy Specialty Chemicals, NC) Dissolved in 5 grams of MIBK. Developing 200 mg 127765.doc •29- 200846661

The agents 4,4, (9.ylidene)-diphenol (available from Alddch Chemicals) and 2 mg of dioctylsulfosuccinate were dissolved in 5 g of MIBK, respectively. The two solutions were mixed together to give a clear, homogeneous solution. Blank indicator tablets were thermally bonded to 4, χ〇 3" polyester sheets by using a supported double-sided PSA to apply Whatman 114 filter paper (from Whatman International, England) to a 3" χ〇·3" sample ( Prepared on one end with 5 mil thick. Use 5 μl of solution to spot on paper. The initial colorless spots turned dark blue after evaporation of the hydrazine. The chemical indicator test piece was dried at 150 Torr for 1 minute and cooled before use. An aqueous solution of n-propanol in a concentration range of 0% w/w/ to 16% w/w. Each of the chemical (tetra) test pieces of the above type was immersed in a solution of n-propanol for 15 seconds under gentle agitation. Each piece was gently blotted onto the absorbent paper towel and quickly measured for optical density on a MaeBeth RD9i7 densitometer. The color intensity was visually discerned as the solvent concentration increased. The results are shown in Figure 4. It shows a linear region of optical density as a function of concentration. Examples are given from the comparison of the water stability of the indicator of Example i and Example 5) The structured chemistry as described in Example 1, Comparative Example! and Example 5. The test piece was immersed in a 12% positive (tetra) aqueous solution for a different length of time between 15 seconds and 2 minutes. Then remove each # from the solution and use a gentle pressure to quickly dry the absorbent paper towel, j^MaeBeth(10)川The optical density was measured immediately on the densitometer. The results are shown in Figure 5, which shows that the chemical indicator test piece prepared using the substantially water-insoluble developer exhibits excellent liquid-liquid stability, and (4) A, hydrophilic developer construction is used. ^ 127765.doc -30- 200846661 The leuco dye complex in the n-type film is rapidly degraded in water. The chemical for the developer is (4) the test piece is immersed by blue "color~, colorless' and other The chemical indicator test piece still retains its color. Example 7 ^

An aqueous solution of n-propanol and propylene glycol (available from AMHh Chemieals) having a concentration ranging from G% w/w/ to 16% w/w was prepared. Chemical indicator test pieces of the type described in Example i were prepared and each immersed in the solutions for 15 seconds under gentle agitation. Each piece was then removed from the solution and gently absorbed using a gentle pressure: the towel was quickly blotted dry and the optical density was measured immediately on a MacBeth RD917 densitometer. It can be visually discerned that the color intensity gradually decreases as the solvent concentration of the propanol solution increases. Even at the highest concentration of propylene glycol used, no change in optical density was observed. The difference in the reactivity of the two closely related alcohols is due to the fact that n-propanol can solvate poly(4-vinylphenol) and propylene glycol. The results are shown in Fig. 6, which shows that the chemical indicator test piece of the present invention can selectively display a color change by using one of the two closely related organic solvents to make a difference in the solvating ability of the developer to be used. Example 8 (Using a substantially water-insoluble leuco dye complex and a water-insoluble adjuvant to construct an indicator sheet) 20 mg of the leuco dye Pergascript Blue I-2RN from Ciba-Geigy Specialty Chemicals, NC was dissolved in 2.5. Ke from Aldrich

Chemicals in decyl isobutyl ketone (MIBK). 200 mg of developer poly(4-vinylphenol), MW = 8000 Da (from Alddch Chemicals) and 200 mg of adjuvant ethionyl tributyl citrate (Citroflex® 4 by Moreflex, NC) were dissolved separately 5 grams in MIBK. The two solutions were mixed together 127765.doc -31 - 200846661 to give a clear homogeneous solution. The blank chemical indicator test piece was thermally bonded to a 4πχ0.3" polyester sheet by using a supported double-sided PSA to transfer Whatman 114(R), paper (from Whatman Initiation, British) to a 0.33" 0.3" sample. Prepared at one end of 5 mil thick). A 5 μl solution was used to spot on the paper. The initially colorless spots turned dark blue after the hydrazine evaporates. The *chemical indicator test piece was dried at 150 °F for 1 minute and cooled. • Comparative Example 2 This example shows the construction of an indicator sheet using a substantially water insoluble anthraquinone dye complex and a water non-soluble non-polar aprotic compound. Will be 20 mg

Pergascript Blue I-2RN is dissolved in 2.5 g of methyl isobutyl ketone (MIBK). 200 mg of poly(4-vinylphenol), Mol.Wt. = 8000 Da and 400 mg of mineral oil (from Cumberland Swan, TN) were dissolved in 2.5 g of MIBK, respectively. The two solutions were mixed together to give a clear, homogeneous solution. The blank indicator film is thermally bonded to a 4" χ〇·3" polyester sheet (5 mil thick) to 10 ends by using a supported double-sided PSA to bond the What3 114 filter paper. And prepared. A 5 microliter solution was used to spot on the paper. At the beginning, the colorless spots turned dark blue after evaporating. The indicator piece was dried at 150 °F for 1 minute and cooled. Example 9. This example demonstrates the use of the chemical indicator test piece of Example 8. An aqueous solution of n-propanol (available from Aldrich Chemicals) having a concentration ranging from 0% w/w/ to 14% w/w was prepared. Chemical indicator test sheets of the type described in Example 8 were prepared and each was impregnated in the solutions for 15 seconds under gentle agitation. Each piece was then removed from the solution and quickly blotted onto the absorbent paper towel using a gentle pressure of '127765.doc -32-200846661 and the optical density was measured immediately on a MacBeth RD917 densitometer using a green filter. It can be visually discerned that the color intensity gradually decreases as the concentration of propanol increases. The graphical representation of the results is shown in Figure 7, which shows the linear dependence of optical density on concentration over the entire analyte concentration range. A chemical indicator test piece of the above type was prepared but without an adjuvant. The tablets were each immersed in a solution of n-propanol for 15 seconds under gentle agitation. Each piece was then removed from the solution, quickly blotted dry onto the absorbent paper towel using gentle pressure, and the optical density was measured immediately on a MacBeth RD917 densitometer using a green filter. It can be visually discerned that the intensity of the color gradually decreases as the concentration of the propanol exceeds a certain value. The results are graphically represented in Figure 8 and the linear range of the curve is extended down to 8% n-propanol. Example 1 〇 (Structural indicator with different hydrophobic polar aprotic chemicals) Test piece and its use 20 t of leuco dye, Pergascript Blue I-2RN, was dissolved in 2.5 g of MIBK. 200 mg of developer poly(4.vinylphenol), M.W. = 8000 Da and 400 mg of adjuvant n-butyl sulfonamide (Aldrich Chemicals) were dissolved in 2.5 gram of < The two solutions were mixed together to give a clear, homogeneous solution. The blank chemical indicator test piece was thermally bonded to a 4" x 3" sample of Whatman 114 filter paper (available from Whatman International, England) using a supported double-sided PSA. A sheet (5 mil thick) was prepared at one end. A 5 microliter solution was used to spot on the paper. The initial colorless spots turn blue after the MIBK evaporates. The indicator piece was dried at 150 °F for 1 minute and cooled. An aqueous solution of n-propanol having a concentration ranging from 0% w/w/ to 16% w/w was prepared. -33- 127765.doc 200846661 A chemical indicator test piece of the above type was prepared and each was impregnated in the solutions for 15 seconds under gentle agitation. Each piece was then removed from the solution, blotted dry on a absorbing tissue using gentle pressure, and the optical density was immediately measured on a MacBeth RD917 densitometer. It can be visually discerned that the color intensity gradually decreases as the solvent concentration increases. The results are shown in Fig. 9. Example 11 (Use of indicator and propylene carbonate analyte in Example 8) An aqueous solution of propylene carbonate (Aldrich Chemicals) having a concentration range of 0% w/w/ to 12% w/w was prepared. Chemical indicator test sheets of the type described in Example 8 were prepared and each immersed in a propylene carbonate solution for 5 seconds under gentle agitation. Each piece was removed from the solution and quickly blotted onto the absorbent paper towel using gentle pressure' and the optical density was measured immediately on a MacBeth RD917 densitometer using a green filter setting. It can be visually discerned that the color intensity gradually decreases as the concentration of propylene carbonate increases. The results are graphically represented in Figure 1 where the linear dependence of optical density on concentration is extended down to approximately 〇% analyte in water. Example 12 20 mg of Pergascript Blue I-2RN (Ciba-Geigy Specialty Chemicals, NC) was dissolved in 5 g of MIBK (AldHch Chemicals). 200 mg of 4,4f-(9-ylidene)-diphenol and 4 mg of sodium dioctylsulfosuccinate (Aldrich Chemicals) were dissolved in 5 g of MIBK, respectively. The two solutions were mixed together to give a single clear homogeneous solution. Blank Chemical Indicator The test piece was prepared from blotting paper No. 703 from VWR Scientific, PA. A 5 microliter solution was used to spot on the paper. The initially colorless spots turned dark blue after the MIBK evaporates. The chemical indicator test piece was dried at 15 Torr for 10 minutes and cooled. 127765.doc -34- 200846661 Example i3 (Detection of fatty acid monoester) Propylene glycol monocaprylate (Abitec, 99% purity, FCC) was prepared at a weight ratio of 98:2 in a concentration range of 〇%~~ to 1% w/w An acidic aqueous solution of a mixture of a dioctyl group and a leuco acid salt (Cytec, FCC grade) and used as an analyte solution. The solution was 0.25% in lactic acid and 25% in malic acid (Brenntag, FCC grade). A chemical indicator test piece of the type described in Example 12 was prepared and spotted on the indicator area with 4 Torr of the analyte solution. Each sheet was then developed at room temperature for 10 minutes and dried at 150 ° for 5 minutes. The optical density of the chemical indicator test strips was measured on a MacBeth RD917 densitometer using a red filter, mirror setting at zero density. It can be visually discerned that the color intensity gradually decreases as the ester concentration increases. The results are graphically represented in the figure, which indicates that the optical density of the chemical indicator test strip is linearly dependent on the aqueous concentration of the monoester and has a large response per unit concentration change. Example 14 20 mg of the leuco dye, Pergascript Blue I-2RN (Ciba-Geigy Specialty Chemicals, NC) was dissolved in 5 g of MIBK (Aldrich Chemicals). 200 mg of the developer poly(4-vinylphenol), MeW. = 8 〇 (10) and 40 g of sodium dioctyl sulfosuccinate (both from Aidrich Chemicals) were dissolved in uMIBK, respectively. The two solutions were mixed together to give a clear, homogeneous solution. Blank chemical indicator test strips were prepared from blotting paper No. 703 from VWR Scientific, PA. Use 5 μl of the solution to spot on the paper. The initially colorless spots turned dark blue after the evaporation of MIBK. The indicator piece was dried at 150 T for 10 minutes and cooled. 127765.doc -35- 200846661 Example 15 Preparation of propylene glycol monocaprylate (Abitec, 99% purity, FCC grade) and dioctyl group at a weight ratio of 98:2 in a concentration range of 〇% w/w to 1% w/w An aqueous acid solution of a mixture of sulfosuccinates (Cytec, FCC grade). The solution was 0.25% in lactic acid and 0.25% in malic acid (Brenntag, FCC grade). A chemical indicator test piece of the type described in Example 13 was prepared and spotted on the indicator area with a 4 Torr microliter test solution. Each sheet was then developed at room temperature for 1 minute and dried at 150 °F for 5 minutes. The optical density of the sheets was measured on a MacBeth RD917 densitometer using a red filter setting at zero density. It is visually discernible that the color intensity decreases extremely gently as the ester concentration increases. The results are shown graphically in Figure 12. Although the features of the present invention have been described in the context of the various embodiments described herein, those skilled in the art will appreciate that changes and modifications can be made to the described embodiments without departing from the spirit and scope of the invention. . BRIEF DESCRIPTION OF THE DRAWINGS In the description of the embodiments of the present invention, reference is made to the drawings in which: FIG. 1 is a graph of optical density and n-propanol concentration when using the chemical indicator test piece described in Example 1 herein; A plot of the optical density versus the concentration of (1) acetone and (2) propylene carbonate using the chemical indicator described in Example 3 herein; Figure 3 is a test strip using the chemical indicator described in Example 4 herein. Graph of optical density and n-propanol concentration; Figure 4 is a graph of optical density and n-propanol concentration when using the chemical indicator test piece described in Example 5 herein; 127765.doc -36 - 200846661 Graph of optical density versus time for the chemical indicator test piece described in Example 6 herein for different developers; Figure 6 is the optical density of (1) propylene glycol and (2) when using the chemical indicator test piece described in Example 7 herein. a graph of the concentration of n-propanol; a plot of optical density versus n-propanol concentration using the chemical indicator test strips described in Example 9 herein; * Figure 8 is a chemical indicator using Example 9 herein. Graph of optical density and n-propanol concentration; and FIG. 9 is a graph of optical density and n-propanol concentration for the chemical indicator test piece of Example 10 herein; FIG. 10 is a chemical indicator using the example of the present invention. Graph of optical density and propylene carbonate concentration during test piece; Figure 11 is a graph of optical density and concentration for the chemical indicator test piece of Example 13 herein; and Figure 12 is a chemical indicator test piece for Example 15 herein. A plot of optical density and concentration. 127765.doc •37-

Claims (1)

200846661 X. Patent Application Range: 1 · A chemical indicator test piece comprising: a substrate; and a coating comprising a leuco dye complex on the substrate, the coating being insoluble in water and reactive with an organic analyte The coating is derived from a solution of a leuco dye and a developer. 2. The chemical indicator test piece of claim 1, wherein the leuco dye is selected from the group consisting of fluorenyl golden amine, thiol leucomorphine, α_ _ unsaturated aryl ketone, aza Azlactone, benzamidine-based leuco methylene blue, benzamidine-based leucoxazine, benzamidine-based leucothiazide, β-unsaturated aryl ketone, basic monoazo dye, biguanide Baserolactone, 1 〇-benzylidene-indole, hydrazine, hydrazine, hydrazine-tetraethyl-3,7-diamino-10 hydrazine, carbazole blue, chromogenic azalide a compound, a crystal violet lactone, a diaryl azlactone, a diphenyl decane, a dithio-glucamine, a bis[biguanidino)ethylidene]tetrahalide lactone, a fluorescent yellow matrix, Fluorescent yellow precursor derivative (for example, 3-dialkylamino-7-dialkylpentyl fluorescent yellow mother), green lactone, 3-(indol-3-yl)-3-(4) - substituted aminophenyl) azlactone, fluorenyl bis(indenyl) ethylene, ruthenium, leuco-gold amine, leuco benzyl methylene blue, crypto-color malachite green, 3·methyl-2,2-spirobis(benzo-[f]-bittenylene), _嗔, azlactone leuco dye, cymidine (phthlans), polystyrene methanol, 8-methoxybenzo 13 and snail snail, rhodamine p indoleamine, snail, and substituted 4,7-diaza Heterolactone, Michler's hydrol p-toluenesulfonate, triarylmethane, triphenylcarbamate (gentian violet and malachite green), sultines, 3,3 - two Fang 127765.doc 200846661 base-3H-2, 1-benzoxazole bromide oxide and a combination of two or more of the former. 3. The chemical indicator test piece of claim 1, wherein the developer comprises a proton donor. 4. The chemical indicator test piece of claim 1, wherein the developer comprises a weak acid selected from the group consisting of bisphenol A, p-hydroxybenzoic acid, methyl p-benzoate, 1, 2 , 3-triazole, 4-hydroxycoumarin derivative and a combination of two or more of the former. 5. The chemical indicator test piece of claim 1, wherein the developer comprises one or more Lewis acids. 6. The chemical indicator test piece of claim 5, wherein the Lewis acid comprises an active clay material selected from the group consisting of: atta Pulgite, acid clay, bentonite (bent〇nhe), Montmorillonite, acid, activated bentonite, montmorillonite, zeolite, halloysite, cerium oxide, aluminum oxide, aluminum sulfate, aluminum phosphate, hydrated dioxide, chlorinated, zinc nitrate, activated kaolin and A combination of two or more of the former. 7. The chemical indicator test piece of claim 1, wherein the developer comprises an organic compound selected from the group consisting of ring-substituted phenol, resorcinol, salicylic acid (for example, 3,5- Bis(α,α,·dimethylbenzyl)salicylic acid; 3,5-bis((γ-methylbenzyl)salicylate) or salicylate and its metal salt. 8. The chemical indicator test piece of claim 1, wherein the developer comprises one or more acidic organic compounds comprising a polymeric material selected from the group consisting of phenolic polymers, alkylphenol acetylene resins, Malay Acid/Pin 127765.doc 200846661 A scented resin, a maleic anhydride with a styrene, an ethylene or vinyl methyl ether, a partially or fully hydrolyzed polymer of a mercapto methylene group and a mixture of two or more of the former. 9. The chemical indicator test piece of claim 1, wherein the developer comprises a phenolic resin or a phenolic compound selected from the group consisting of 4-tert-butylphenol, 4-phenylphenol, methylene - bis(p-phenylphenol), 4-hydroxydiphenyl ether, α-naphthol, β-naphthol, methyl 4-hydroxybenzoate, benzyl 4-benzobenzoate, 4-hydroxydiphenyl Sulfone, 4-hydroxyacetophenone, Di-diphenyl, 4,4'-cyclohexylidene, 4,4,-isopropylidene, 4,4-isopropylidene bis(2-methylphenol), thiocyanate Zinc pyridine complex, 4,4-bis(4-hydroxyphenyl)pentanoic acid, hydroquinone, pyrogallol, resorcinol, p-hydroxybenzoic acid, m-hydroxybenzoic acid, adjacent - Hydroxy acid, gallic acid, 1-hydroxy-2-naphthoic acid. 10. The chemical indicator test piece of claim 1, wherein the developer is poly(heart vinylphenol). 11. The chemical indicator test piece of claim 1, wherein the developer is (9-arylene)-diphenyl. 12. The chemical indicator test strip of claim i, wherein the coating further comprises an adjuvant. 13. The chemical indicator test strip of claim 12, wherein the adjuvant is a water-insoluble, polar, hydrophobic, aprotic material that expands the T-limit of the analyte to below the limit. Agent does not 仏 丨 丨 π μ 1" after the servant of the adjuvant includes to 14 · If the chemical indicator test piece of the item 12 is 127765.doc 200846661 less aliphatic or aromatic ester of citric acid, the ester Is selected from the group consisting of citrate, phthalate, adipate, benzoic acid vinegar, sebacate, hexaphthalate, and combinations of two or more of the former. The chemical indicator test strip of claim 12, wherein the adjuvant is selected from the group consisting of organophosphates, organic sulfonates, sulfonamides, and combinations of two or more of the former. 16. The chemical indicator test strip of claim 12, wherein the adjuvant is selected from the group consisting of citric acid esters and sulfonamides, and combinations thereof. 17. The chemical indicator test strip of claim 1, wherein the coating further comprises a surfactant, and wherein the coating is derived from a solution of a synergic dye, a display agent, and a surfactant. 18. The chemical indicator test piece of claim 17, wherein the surfactant is free from the group consisting of an anionic surfactant, a cationic surfactant, a nonionic surfactant, an amphoteric surfactant, and two: An ionic surfactant and a combination of two or more of the former. 19. Test the fatty acid mono- vinegar as requested in the chemical indicator. An organic analyte system. A method of using a chemical indicator test piece, comprising: (4) exposing a chemical indicator test piece to a liquid sample including the solution analyte; the chemical indicator test piece comprises: an organic substrate And the coating comprising the leuco dye complex on the substrate is dissolved in water to react with the money analyte without increasing the color of the 127765.doc 200846661 solution of the material and the developer; (b) measuring The chemical indicator test color change on the sheet after the exposure step; (c) correlating the color change with the concentration of the analyte in the sample. 21. The method of claim 20, further comprising recording the concentration of the analyte. 22. The method of claim 20, further comprising establishing a standard relationship between the color change on the chemical indicator test piece and the analyte concentration in the sample. The method of claim 20, wherein the leuco dye is selected from the group consisting of fluorenyl golden amine, thiol leucophthyl azine, α-unsaturated aryl ketone, azalide, benzene Nail-based leuco methylene blue, benzamidine-based leuco-smell, snail-based sigma syrazine, β-unsaturated aryl g, iso-initial monoazo dye, bis-indolyl Ester, 1〇_benzamide*_n, n, n, n•tetraethyl-3, diamino-10H-phrotazine, carbazole blue, chromogenic azlactone compound, crystal violet lactone , diaryl azlactone, diphenyl decane, dithio-glucamine, bis [bis-(indenyl) extended ethyl] tetra-sodium lactone, fluorescent yellow matrix, fluorescent yellow The parent derivative (for example, 3-dialkylamino-7-dialkylpentyl fluorescent yellow precursor), green lactone, 3-(indenyl)_3_(4_substituted aminophenyl) azlactone , 吲哚基双_(吲哚基) ethylene, 吲. Wood red, leuco-gold amine, leucophthalic acid methylene blue, leuco malachite green, 3-mercapto-2, 2-spiro (benzoxene), brown cockroach, vinegar vinegar Dyes, eucalyptus, polystyrene, sterol, porphyrin and porphyrin, spiroxane, rhodamine beta decylamine, spiropyran, substituted 4,7-diazaindole Lactone, m-alcohol p-toluene acid ester, triaryl decane, triphenyl 127765.doc 200846661 base carbaryl (gentian violet and malachite green), sutidine, 3,3-diaryl-3H- 2,1-Benzoxoxazole 1 ·oxide and a combination of two or more of the former. The method of claim 2, wherein the developer comprises a proton donor. 25. The method of claim 20, wherein the developer comprises a weak acid selected from the group consisting of bisphenol A, octyl p-hydroxybenzoate, methyl p-hydroxybenzoate, 1,2,3-triazole, A 4-hydroxycoumarin derivative and a combination of two or more of the former. 26. The method of claim 20, wherein the developer comprises one or more Lewis acids. The method of claim 2, wherein the Lewis acid comprises an active clay material selected from the group consisting of: green Palygorskite, acid clay, bentonite, montmorillonite, acid_activated bentonite, montmorillonite, zeolite, halloysite, oxidized slag, oxidized sulphur, sulphuric acid, sulphuric acid, hydrated dioxide dream, zinc sulphide , zinc nitrate, activated kaolin and a combination of two or more of the former. The method of claim 20, wherein the developer comprises an organic compound selected from the group consisting of cyclo-substituted phenol, resorcinol, salicylic acid (eg, '3,5-bis(α, α'_Dimethylbenzyl) salicylic acid; 3,5_biguanide-methyl group) salicylic acid)) or salicylate and its metal salt. The method of claim 20, wherein the developer comprises one or more acidic organic compounds comprising a polymeric material selected from the group consisting of squaring polymers, alkyl phenol acetylene resins, maleic acid/rosin resins a partially or fully hydrolyzed polymer of maleic anhydride with styrene, ethylene or vinyl methyl ether, a carboxymethylene group, and a mixture of two or more of the former. The method of claim 20, wherein the developer comprises a phenolic resin or a phenolic compound selected from the group consisting of: 4·t-butylphenol, 4-phenylphenol, Methylene-bis(p-phenylphenol), 4-hydroxydiphenyl ether, α_naphthol, β-naphthol, decyl 4-hydroxybenzoate, benzyl 4-hydroxybenzoate, 4-hydroxyl Phenylhydrazine, 4-hydroxyacetophenone, 2,2,-dihydroxydiphenyl, 4,4'-cyclohexylene phenol, 4,4' isopropylidene diphenol, 4, 'isopropylidene Bis(2-methylphenol), pyridine thiocyanate complex, hepta-7-bis(dehydroxyphenyl)pentanoic acid, hydroquinone, pyrogallol, phloroglucinol, p- Hydroxybenzoic acid, m-hydroxybenzoic acid, o-hydroxybenzoic acid, gallic acid, 1-hydroxy-2-naphthoic acid. The method of claim 20, wherein the coating further comprises an adjuvant. The method of claim 20, wherein the adjuvant is water insoluble, polar, & water non-shell material, which can expand the lower limit of detection of the analyte to be lower than when the adjuvant is not present The method of claim 20, wherein the adjuvant comprises at least one aliphatic or aromatic ester of a carboxylic acid selected from the group consisting of citric acid _, phthalic acid A combination of vinegar, adipic acid _, benzoic acid vinegar, azelaic acid vinegar, benzoic acid ester, and a combination of two or more of the foregoing. The method of claim 20, wherein the adjuvant is selected from the group consisting of a group of organophosphates, an organic sulfonate, a sulfonamide, and a combination of two or more of the former. 35. The method of claim 20, wherein the adjuvant is selected from the group consisting of citric acid vinegar and sulfonamide The method of claim 20, wherein the organic analyte is a fatty acid monoester. The method of claim 20, wherein the coating further comprises a surfactant. And wherein the coating is a solution of a substrate/surfactant and a surfactant. The method of claim 22, wherein the surfactant is selected from the group consisting of Group anionic surfactant, cation # ^ Risk ionic surfactants, nonionic surfactants, two men and thumb, and ^ W is a combination of ceramic winter surfactant to zwitterionic surfactants of two or more of the former. 127765.doc