200827697 九、發明說明： . * . . ... . 【發明所屬之技術領域】 一種微塑取樣 本發明是有關於一種取樣器，特別是才t 器/ 【先前技術】 在許多科技領域中，尤其是生物、化學等似 ^ ^ ^ ^ ^ ^ ^ ^ * 0, S ^ ^ # ^ ^ ^ ^200827697 IX. INSTRUCTIONS: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Especially biological, chemical, etc. ^ ^ ^ ^ ^ ^ ^ ^ * 0, S ^ ^ # ^ ^ ^ ^
^ ^t 4 (^ ^t 4 (
Pipeue)進行取樣，此種取樣裝置通常是將一吸管頭套設於 一吸引取樣器上，藉由取樣器内部構輕 ^ ^ ^ t ^ ^ ^ ^ ^ f ^ t 〇 ^ 0 ^ ^ ^ #^ ^ 樣器上時，可能會因套糊 ^ ^ ^ ^ ^ ^ ^ ^ ^ f ^ # J, , ^ ^ ^ ^ # ^ # 4 ^ ，逢度，尤其是對於極微量樣品的取樣時，例如小於、X ]『 9公升（nano liter, nl)，其影響程度更大。 【發明内容】 .. - · . 因此，本發明之目的，即在提供一種可精確擷取多種 不同體積之樣品量之微型取樣器。 一；=是，本發明微型取樣器，包含由上往下依序疊接之 =乳室成型板、一流道成形板與一基板。該流道成型板是 =接於乳室成型板底面，並與氣室成型板相配合界定出一 第尸 第一與一第三閥門氣室，並具有一集液孔、一取 ^氣孔，及分別對應位於該等閥門氣室下方之一第一、一 第二與一第三閥門部。該基板是疊接於流道成型板底面， 200827697 並與流道《板_合界^-連通於取樣減心液孔 間且可使取樣液流經第三與第二閱門部下方而流^液孔 之取樣槽道、一與取樣槽道位於第-盥黧一p ^ m FU卩下方^取樣槽道 之引流道，及至少-㈣ 反側之區段間的吸引通道。 11¾ f N H a ^ ^ ^ ^ n af , ^ f ^ f1 ^Pipeue), the sampling device usually sets a straw head on a suction sampler, and the internal structure of the sampler is ^ ^ ^ t ^ ^ ^ ^ ^ f ^ t 〇 ^ 0 ^ ^ ^ #^ ^ When the sample is on, it may be due to the set ^ ^ ^ ^ ^ ^ ^ ^ ^ f ^ # J, , ^ ^ ^ ^ # ^ # 4 ^ , 逢, especially for the sampling of very small samples, for example Less than, X ] 『 9 liter (nano liter, nl), the degree of influence is greater. SUMMARY OF THE INVENTION Therefore, it is an object of the present invention to provide a microsampler that can accurately capture a plurality of sample volumes of different volumes. One; = Yes, the microsampler of the present invention comprises a milk chamber forming plate, a first-stage forming plate and a substrate which are sequentially stacked from top to bottom. The flow channel forming plate is connected to the bottom surface of the milk chamber forming plate, and cooperates with the gas chamber forming plate to define a first corpse first and a third valve air chamber, and has a liquid collecting hole and a gas collecting hole. And corresponding to one of the first, second and third valve portions below the valve chamber. The substrate is superposed on the bottom surface of the flow channel forming plate, and is connected to the flow channel "plate_bound" to communicate with the sampling centering hole and allows the sampling liquid to flow through the third and second reading portions. The sampling channel of the liquid hole, the sampling channel of the sampling channel located below the first - p ^ m FU , the sampling channel of the sampling channel, and the suction channel between the sections of the at least - (four) opposite side. 113⁄4 f N H a ^ ^ ^ ^ n af , ^ f ^ f1 ^
^ ^ ^ ^ H ^ ^ ^ # ^ ^ ^ ^ ^ ?1 ^ ^ ^ 取樣槽道，當該取樣氣孔之空氣被抽離時，舊料 吸引通道會產生-將引流道中之取樣液吸 將位於取樣槽道中之取樣液沖入集液孔中。 【實施方式】 有難發隊魏及麵技術时^御^ ^ ^ ^ H ^ ^ ^ # ^ ^ ^ ^ ^ ? 1 ^ ^ ^ Sampling channel, when the air of the sampling vent is pulled away, the old material suction channel will be generated - the sampling liquid in the drainage channel will be located The sample liquid in the sampling channel is flushed into the sump. [Embodiment] When there is a difficult team, Wei and Noodle Technology
^ T ^ ^ Ψ Μ Λ ^ - ^ ^ ^ ^ ^ m n ^ n t > Jf T 清楚的呈現。 -.. .. -. · ..... -- ..... . . ·' . _ 如圖1、2所示r本發明微型取樣器之較隹實施例適用 之一水平基板3、一水平流道杰别士 现逗成型板4及一水平氣室成型板 5ι該基板3是由則製成，㈣施^該基板，亦可由其 他硬質材料製成，且不以此為限。 該流道成型板4是由彈性材料製成，且與疊接於其底 面之基板3相配合界定出—左右延伸之取樣槽道仙、一前 後延伸独其前端連通於取樣舰4G右端部之㈣道41、 200827697 - 三左右間隔且分別與取樣槽道40連通之吸引通道42，及一 連通於取樣槽道40左端且貫穿頂底面之第一取樣氣孔43。 並具有一貫穿其頂底面且與該引流道41後端連通之第一儲 液孔44、一貫穿其頂底面且連通於取樣槽道40右端之第一 集液孔45、四間隔位於該取樣槽道4〇與該引流道々I上方 之閥門部46，及一突伸入取樣槽道4〇中之止逆部们。在 : 本實施例中，該流道成型板4是由PDMS製成，但實施時 不以此為限。 該取樣槽道40具有由右往左依序相連之一第―、一第 二與一第三取樣段401、4〇2、4〇3、一自第一取樣段4〇r ..... .. 端往右延伸連通該第一集液头45的第一連通段4〇4，及一 自第三取樣段4Ό3左端往後再往左延伸連通該第一取樣氣 ... . . ... .... 孔43之第二連通段4〇5。在本實施例中’第一取樣段 之容積為0.02 nl，第一與第二取樣段4〇1、4⑽ 0.1 η卜第一、第二與第三取樣段4〇1〜4〇3之總容積為〇.6 · η1，但實施時，該等取樣段4〇1〜4〇3之容積設計不以上述為 限。 -... .... ..... . ... 該引流道41是與第一取樣段401連通，而該等閥門部 46是分別位於該等取樣段4〇1〜4〇3連通處與該引流道41正 • . . - - · · 上方’為方便說明，將位於引流道41正上方之閥門部46 、 稱為第一閥門部，將位於取樣槽道4〇上方之閥門部46由 r 右至左依序區分為第二、第三與第四閥門部。 為方便說明，將該等吸引通道42由右往左依序區分為 第一、第二與第三引流通道，其中，第一引流通道42是連 200827697 通於第一取樣段401與第二取樣段402間，第二引流通道 42是連通於第二取歡段4〇3與第三取樣段4〇3間，第三引 流通道42是連通於第三取樣段403與第二連通段405間， .... '- ' 、 ..... 且該等引流通道42用以和該等取樣段401〜403連通之區段 的管徑皆遠小於讓等取樣段401〜403。 该止逆部47是自第二連通段405的右側壁往左突伸，^ T ^ ^ Ψ Μ Λ ^ - ^ ^ ^ ^ ^ m n ^ n t > Jf T Clear presentation. -.. .. -. · ..... - ..... . . . '. _ As shown in Figures 1 and 2, a horizontal substrate 3 is applied to the comparative embodiment of the microsampler of the present invention. a horizontal flow channel, a singularly shaped plate 4, and a horizontal air chamber forming plate 5, the substrate 3 is made of (4) the substrate, and may be made of other hard materials, and is not limited thereto. . The flow path forming plate 4 is made of an elastic material and is defined to cooperate with the substrate 3 stacked on the bottom surface thereof. The sampling channel is extended to the left and right, and the front end is connected to the right end of the sampling ship 4G. Lanes 41, 200827697 - three suction channels 42 spaced apart from each other and spaced from the sampling channel 40, and a first sampling air hole 43 communicating with the left end of the sampling channel 40 and extending through the top surface. And having a first liquid storage hole 44 extending through the top surface of the bottom surface and communicating with the rear end of the drainage channel 41, a first liquid collecting hole 45 penetrating through the top surface of the bottom surface and communicating with the right end of the sampling channel 40, and the four intervals are located in the sampling The channel 4〇 and the valve portion 46 above the channel 々I, and a stop portion projecting into the sampling channel 4〇. In the present embodiment, the flow path forming plate 4 is made of PDMS, but is not limited thereto. The sampling channel 40 has one from the right to the left, a second and a third sampling segment 401, 4〇2, 4〇3, and a first sampling segment 4〇r .... The end extends to the right to communicate with the first connecting section 4〇4 of the first liquid collecting head 45, and the left end of the third sampling section 4Ό3 extends back to the left to communicate with the first sampling gas. . . . The second connecting section 4〇5 of the hole 43. In the present embodiment, the volume of the first sampling section is 0.02 nl, and the total of the first and second sampling sections 4〇1, 4(10) 0.1 ηb, the first, second and third sampling sections 4〇1~4〇3 The volume is 〇.6 · η1, but in practice, the volume design of the sampling sections 4〇1~4〇3 is not limited to the above. -...................... The drain channel 41 is in communication with the first sampling section 401, and the valve sections 46 are located in the sampling sections 4〇1~4〇3, respectively. The communication portion and the drain passage 41 are positive. . . - - · · Above 'For convenience of explanation, the valve portion 46 located directly above the drain passage 41, referred to as the first valve portion, will be located above the sampling channel 4〇 The portion 46 is sequentially divided into second, third, and fourth valve portions from right to left. For convenience of description, the attracting channels 42 are sequentially divided from right to left into first, second, and third drainage channels, wherein the first drainage channel 42 is connected to the first sampling segment 401 and the second sampling. The second drainage channel 42 is connected between the second sampling section 4〇3 and the third sampling section 4〇3, and the third drainage channel 42 is connected between the third sampling section 403 and the second connecting section 405. , .... '- ', ..... and the diameters of the sections of the drainage channels 42 for communicating with the sampling sections 401 to 403 are far smaller than the sampling sections 401 to 403. The non-return portion 47 protrudes to the left from the right side wall of the second communication portion 405.
但其左端並未與第二連通段405左側壁固接，而與左側壁 間隔30# m。 琢氣至成型板5是由彈性材料製成，且與叠接於其底 面之流道成型板4相配合界定出四僻分別位於該等閥門部 正上方之閥門氣至51 ’並具有四分別和該等閥門氣室5! 連通並*伸至其頂面之通氣孔52、_與第，儲液孔从連通 並延伸至咖 ^ ^ H ® ^ f ^ # ^ 54 , ^ # ^ ^ ^ ^^ 43However, the left end thereof is not fixed to the left side wall of the second connecting portion 405, but is spaced apart from the left side wall by 30# m. The helium gas to the forming plate 5 is made of an elastic material, and cooperates with the flow channel forming plate 4 which is superposed on the bottom surface thereof to define four valve valves respectively located above the valve portions to 51' and has four respectively Connect with the valve chamber 5! and extend to the top surface of the vent hole 52, _ and the first, the liquid storage hole from the connection and extend to the coffee ^ ^ H ® ^ f ^ # ^ 54 , ^ # ^ ^ ^ ^^ 43
it ^ ^ ^ Φ ^ ^ ― ^ 5 5 r ^ rn ^ M ^ n ^ t 51 ^ ^ ^ ^ ^ ^ f W ^ H ^ 46 上方之第—〜第四閥門部4 由PDMS製成，但實施時㈣ 本發明微型取樣器使用前，需弁田认 體之#置Γ Η去-、 ^ 先 以輸出高壓氣 壓翕體且可也成产 丹將 了輸出面 整孔體了抽及氣體之裝置（圖 通接 第二取樣氣孔43、ss、 」運通組接於弟一與 4〇中，或將取輕揭，而可將高I氣體灌注於取樣槽道 將取樣槽道4以之空氣抽離，並可藉由-自動控 200827697 制設備（圖未示)程式化控制該等裝置^ 氣等動作。但實施時不以此為限…抽 配合I3所示，敝 動時’需先對第 門部1往下彈㈣仲人引流以 41 ’接者便可將待取樣之溶液注入談等儲液孔44、53中。It ^ ^ ^ Φ ^ ^ ― ^ 5 5 r ^ rn ^ M ^ n ^ t 51 ^ ^ ^ ^ ^ ^ f W ^ H ^ 46 The upper - fourth valve part 4 is made of PDMS, but implemented (4) Before using the microsampler of the present invention, it is necessary to set up the high pressure air pressure body and the output of the whole surface of the output body. (The figure is connected to the second sampling air hole 43, ss, and the transport group is connected to the first and fourth sides, or the light is removed, and the high I gas can be poured into the sampling channel to pump the sampling channel 4 to the air. It can be programmed to control the operation of these devices by means of automatic control of the 200827697 system (not shown). However, the implementation is not limited to this... The pumping is matched with I3, and when it is moved, it needs to be The first part of the door 1 is pulled down. (4) The secondary person is diverted to the fourth person to inject the solution to be sampled into the liquid storage holes 44, 53.
配合圖4所示，當所需溶液量為〇.〇2^時，亦_ 取樣段401之容積量時，可再於第二與第三_ 灌注高壓氣體，迫使第二與第三閥門部46分別彈桂突伸入 取樣槽道4〇中’而分別氣密阻塞於第一連通段4〇4與第一 取樣段401間，及阻塞於第一與第二取樣段4〇1、4〇2間。 部46彈性復位而打開引流道41 ...·· . . · . 然後，便可將第一閥門氣室51中之氣體棑出，使第一閥門 接著，再經由該等取樣氣孔43、5 5而將預定體積 氧抽離，利用該等取樣氟孔43、55内空氣被抽離而產生之 負壓吸力，將第二、第三取樣段4〇2、4〇3與第一吸引通道 42中之預定體積的空氣，經由該止逆部47與左侧壁面之間 隙吸走，而相對於第二、第三取樣段4〇2、4〇3與 通道42中產生一負壓吸力，由於第一吸引通道42是連通 於第二與第一取樣段402、401間，且第一閥p]部46已被 打開，所以經由第一吸引通道42而於第一取樣段4〇1中產 . - - t . . . . 生之負壓吸力，會將該等儲液孔44、53中之預定體積的溶 液經由引流道41吸入第一取樣段401中。 當溶液樣品逐漸被吸入第一取樣段4〇1後，整個取樣 200827697 ν' 、. ... . . • .· · · ’ - · - . · . - · · ·--- - . .. ...:. : -… .- . : . : · . : * 槽道40之負壓吸力會逐漸變小而消失，此時，吸人取樣槽 .· - . ; ' / ' . · . ... . - - ' · : 道40中之溶’液樣品量會剛好填滿整個第一取樣段4〇1，且 - -' - - - . ·· 該第一取樣段401中之溶液量即為0 02 ηι。 ..... ... . .. .. . . 接著，將第二與第三閥門氣室5〗中之空氣排除，使該 等閥門部46彈性復位，而使第一取樣段401與第一連通段 ...'_ . .... . . . ' . - . ' . '1 : . _ . · : 1, ...... 404連通。最後，再快速地對該等取樣氣孔43、55灌注預 _ 一流向該等集液孔45、54之 401中之溶液快速地推入集液孔45、54中，便可於談等集 液孔45、54中獲得所需體積之溶液樣品。 -_. ... … · -. - . ； -. · .... · - 同樣的取樣方式，當所需溶液樣品量為0·1η1，亦即為 第一與第二取樣段401、4〇2之總容積量時，可先迫使第二 與第四閥門部46分別氣密阻塞於第一取樣段4〇1與第一連 通段404間及第二與第三取樣段4〇2、4〇3間，再將第一闕 門部46打開，使儲液孔44、53中之 • . . . ... . - ' - - · · ⑩ 流入取樣槽道40中。完成上述步驟後，便可於該等取樣氣 孔43、:55、取樣槽道4〇與第二吸引通道42產生負壓吸力 ’因该第二吸引通道42是連通於第一與第二取樣段4〇1、 1〇2間，所以該負壓吸力會將該等儲液孔44、53中之溶液 吸入填滿第一與第二取樣段401、4〇2。然後，再打開第二 與第四閥門部46，並藉由在該該等取樣氣孔43、55中灌注 - - ； . ... . 1 间壓氣體’使所產生之高速氣流推力，將取樣槽道40中之 取樣溶液推入該集液孔45、54中，而完成取樣動作。 - - . · 若/谷液取樣量為〇_6 nl，亦即第一〜第三取樣段4〇1〜4〇3 10 200827697 ^ - ... - . :-- .... - ·- ' ::' . . . . . . ... .: · ' ...... . .:. -_ . -. - · . . . ' - .. - . . - . 之總容積量時，其取樣方式亦相同，只是取樣前，僅會迫 • . . · - · 使第二閥門部46氣密阻塞於第一取樣段401與第一連通段 404間’再使第一閥門部46打開，使儲液孔44、53中之溶 液可流入取樣槽道40中。並藉由負壓吸力，將該等儲液孔 44、53中之溶液樣品吸入填滿第一〜第三取樣段4〇1〜4〇3。 - ' - . - ...... ; . · 然後，再打開第二閥門部46，並將取樣槽道40中之取樣溶 … 液推入該集液孔45、54中。當所需取樣量大於該等取樣段 • - _ . . - . - * . - .... .. • 401 403之總谷積時，則可分成多次進行取樣。 值得一提的是，在取樣過程中，由於引流道41已被第 一閥門部46氣密阻塞住，所以當氣流沿第一取樣段4〇1壁 面快速通遇垂直向之引流道41開口時，該高速氣流會於該 引流道41開口形成一液體戴斷面，並不會帶走殘留於引流 道41中之溶液，因此，可精確控制取樣量。 . .. .. . . ... - : . . 在本實施例中，該取樣槽道4〇僅規劃三取樣段 401〜4〇3 ,但實施時，可依需要而改變取樣段數量，且對應 _ 改變談等吸引通道42之數量，及該等閥門部46與閥門氣 室5 1之數量，使相連通之相鄰取樣段間皆連通有—吸引通 道42，而每一取樣段連揍處上方皆有一閥門部46與一閥門 氣室51 〇 本實施例所取之溶液樣品是被集中在集液孔45、54中 - …但實施時’亦可不設置第二集液孔54，而直接於第一集 液孔45、’a接引4官（圖未示），直接將被排出取樣槽道 4〇之樣品傳送至後端其他晶片或儀器(圖未示）進行^測 200827697 · . . - . .. .... / . ... ... ...... .. . .. • 連通段404右端之分流官道（圖未示），使樣品可被控制而 流至預定之分流管道中，由於分流方式並非本發明之設計 重點，因此不再詳述，且實施時不以上述型態為限。' ..... - ” . ' ... . -. ' . . 歸納上述，透過談等取樣槽道4〇、闊門部46、闕門氣 至51、取樣氣孔43、55與吸引通道42的結構設計，以及 〇等閥門部46與閥_室〔义^^ 取樣器可精確地取得多種極微量體積之溶液樣品,相 _ : t方便實用。因此，蜂實可達到本發明之目的。 准以上所述表，僅為本發明之一較佳實施例而已，當 不能以此限定本發明實施之範圍，即大凡依本發明申請專 1範圍及發明說明内容所作之㈣^ 仍屬本發明專利涵蓋之範圍内。 【圖式簡單說明】 … ... ...... ： -. ： -- -- · - - _ - . . ' * ' ... .· . - 胃.圓1是本發明微型取樣器之__較佺實施例的立體分解 —3是圖2沿υ線之侧視剖面圖，說明一引流道被 —弟一閥門部氣密祖塞時的情況;及 ' - . . 、. * ' . . - · ：' * …_圖4疋圖2沿1^汉線的侧視剖面圖，說明一第二與一 : 弟三閥門部分別阻塞於取樣槽道中情況。 ’、 12 200827697 【主要元件符號說明】 3 ....... …基板 44····· ....第一儲液槽 4 ....... …流道成型板 45"··· ....第一集液槽 40···… ...取樣槽道 46..... ....閥門部 401 .... …第一取樣段 47.·.·· ....止逆部 402·.·· …第二取樣段 5 .... ....氣室成型板 403···· …第三取樣段 51"… ….閥門氣室 404.... ...第一速通段 52.;... •...通氣孑L 405···· ...第二連通段 53·"·· ….第二儲液孔 41...... …引流道 54".·· ….第二集液孔 42...... …吸引通道 5 5..... ....第二取樣氣 43 ···. …第一取樣氣孔 13As shown in Fig. 4, when the required amount of solution is 〇.〇2^, and _ the volume of the sampling section 401, the second and third _ can be filled with high-pressure gas to force the second and third valve parts. 46 respectively, the keloid protrudes into the sampling channel 4' and is hermetically sealed between the first connecting section 4〇4 and the first sampling section 401, and is blocked in the first and second sampling sections 4〇1. 4〇2 rooms. The portion 46 is elastically reset to open the drain passage 41 ..... Then, the gas in the first valve chamber 51 can be scooped out, so that the first valve is followed by the sampling holes 44, 5 5, the predetermined volume of oxygen is withdrawn, and the second and third sampling sections 4〇2, 4〇3 and the first suction channel are utilized by the negative pressure suction generated by the air in the sampled fluorine holes 43, 55. a predetermined volume of air in 42 is sucked away through the gap between the non-return portion 47 and the left side wall surface, and a negative pressure suction force is generated in the second and third sampling sections 4〇2, 4〇3 and the passage 42, Since the first suction passage 42 is in communication with the second and first sampling sections 402, 401, and the first valve p] portion 46 has been opened, it is produced in the first sampling section 4〇1 via the first suction passage 42. - - t . . . The negative suction force of the raw ones sucks a predetermined volume of the solution in the liquid storage holes 44, 53 into the first sampling section 401 via the draining path 41. When the solution sample is gradually taken into the first sampling section 4〇1, the whole sample is 200827697 ν', . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...:. : -... .- . : . : . . : * The suction pressure of the channel 40 will gradually become smaller and disappear. At this time, the sampling slot is sucked. · - . ; ' / ' . ... . - - ' · : The amount of the solution in the channel 40 will just fill the entire first sampling section 4〇1, and - -' - - - - · · The solution in the first sampling section 401 The amount is 0 02 ηι. ..... ... . . . . . . . . Next, the air in the second and third valve chambers 5 is removed, the valve portions 46 are elastically reset, and the first sampling section 401 is The first connected segment...'_ . . . . . . . ' . - . ' . ' . : . . . . Finally, the solution holes 501, 55 are quickly poured into the sump holes 45, 54 of the sump holes 45, 54, and the liquid concentrating holes can be quickly collected. A sample of the desired volume of solution is obtained in wells 45,54. -_. ... ... · -. - . ; -. · .... · - The same sampling method, when the required solution sample amount is 0·1η1, that is, the first and second sampling segments 401, When the total volume of the volume is 4〇2, the second and fourth valve portions 46 may be forced to be airtightly blocked between the first sampling section 4〇1 and the first communication section 404 and the second and third sampling sections 4〇, respectively. 2, 4〇3, and then the first door portion 46 is opened, so that ... . . . . - ' - - · · 10 in the liquid storage holes 44, 53 flows into the sampling channel 40. After the above steps are completed, a vacuum suction force can be generated in the sampling holes 44, 55, the sampling channel 4, and the second suction channel 42. The second suction channel 42 is connected to the first and second sampling segments. 4〇1, 1〇2, so the negative pressure suction will suck the solution in the liquid storage holes 44, 53 to fill the first and second sampling sections 401, 4〇2. Then, the second and fourth valve portions 46 are opened again, and the high-speed airflow thrust generated by injecting - (1 . . . 1 ) pressure gas in the sampling holes 44, 55 will be sampled. The sampling solution in the channel 40 is pushed into the liquid collecting holes 45, 54 to complete the sampling operation. - - . · If the sample volume of 谷谷液 is 〇_6 nl, that is, the first to third sampling sections 4〇1~4〇3 10 200827697 ^ - ... - . :-- .... - · - ' ::' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . When the amount is measured, the sampling method is also the same, except that before the sampling, only the second valve portion 46 is hermetically blocked between the first sampling section 401 and the first connecting section 404. The valve portion 46 is opened to allow the solution in the reservoir holes 44, 53 to flow into the sampling channel 40. The sample of the solution in the liquid storage holes 44, 53 is sucked into the first to third sampling sections 4〇1 to 4〇3 by vacuum suction. - ' - . - . . . . Then, the second valve portion 46 is opened again, and the sampling solution in the sampling channel 40 is pushed into the liquid collecting holes 45, 54. When the required sampling amount is larger than the sampling segments • - _ . . - . - * . - .... .. • When the total volume of 401 403 is accumulated, it can be divided into multiple sampling. It is worth mentioning that, during the sampling process, since the drain passage 41 has been hermetically blocked by the first valve portion 46, when the airflow rapidly meets the opening of the vertical guide passage 41 along the wall of the first sampling section 4〇1 The high-speed airflow will form a liquid-wearing cross section at the opening of the draining passage 41, and will not carry away the solution remaining in the draining passage 41, so that the sampling amount can be accurately controlled. . . . . . . . . In the present embodiment, the sampling channel 4〇 only plans three sampling segments 401~4〇3, but in implementation, the number of sampling segments can be changed as needed. And corresponding to _ change the number of suction channels 42 and the number of the valve portion 46 and the valve chamber 5 1 so that the adjacent sampling segments connected to each other are connected with a suction channel 42 and each sampling segment is connected Above the crucible, there is a valve portion 46 and a valve chamber 51. The solution sample taken in this embodiment is concentrated in the liquid collection holes 45, 54 - but in practice, the second liquid collection hole 54 may not be provided. And directly to the first liquid collection hole 45, 'a lead 4 official (not shown), directly transfer the sample discharged from the sampling channel 4〇 to the other chip or instrument at the back end (not shown) for testing 200827697 · . . - . . . . . / . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . However, the flow is not limited to the design of the present invention, and is not limited to the above-mentioned type. ' ..... - ” . ' . . -. ' . . In the above, by talking about the sampling channel 4, the wide door 46, the door gas to 51, the sampling holes 43, 55 and the suction channel The structural design of 42 and the valve unit 46 and the valve chamber can accurately obtain a variety of extremely small volume solution samples, and the phase _: t is convenient and practical. Therefore, the bee can achieve the purpose of the present invention. The above-mentioned table is only a preferred embodiment of the present invention, and the scope of the present invention cannot be limited thereto, that is, it is still in accordance with the scope of the invention and the description of the invention. Within the scope of the invention patent. [Simple description of the diagram] ... ... : :. : -- -- · - - _ - . . ' * ' ... . . . - stomach. Circle 1 is a three-dimensional decomposition of the microsampler of the present invention. 3 is a side cross-sectional view of the second embodiment of FIG. 2 along the squall line, illustrating a case where a drain channel is airtightly embedded by a valve portion; And '- . . , . * ' . . - · : ' * ... _ Figure 4 疋 Figure 2 along the 1 ^ Han line side cross-sectional view, illustrating a second and one: the third three valve parts are blocked in the sampling tank Middle feelings ', 12 200827697 [Description of main component symbols] 3 ....... ...substrate 44·····....first reservoir 4.........flow channel forming plate 45" ;··· .... first sump 40···... sampling channel 46....... valve part 401 .... ... first sampling section 47.. ···.Reverse part 402·.....the second sampling section 5 ........air chamber forming plate 403····...the third sampling section 51"...the valve chamber 404....the first speed passage 52.;...the...ventilation孑L 405····...the second connected section 53·"··....the second stock solution Hole 41... ...drainage path 54"......Second liquid collection hole 42.........sucking passage 5 5......second sampling gas 43 · ··. ...first sampling air hole 13