200819198 九、發明說明： 【發明所屬之技術領域】 本發明關於一種具有多槽道設計的液體處理移液管。 【先前技術】 現代的實驗室譬如臨床和生物實驗室經常需要同時進行 大量檢驗作業。多孔板當今受到廣泛使用以有效地處理此 種程序。習知多孔板可有一 24個孔穴、96個孔六或384個 孔穴的組態且被配置成一具有一固定中心間距（槽道間距） 的矩形組態。舉例來說，一 24孔的多孔板經配置成一槽道 間距為18 mm的6x4陣列，一 96孔的多孔板經配置成一槽道 間距為9 mm的12x8陣列，而一 384孔的多孔板經配置成一 槽道間距為4.5 mm的24χ 16陣列。 液體處理作業在絕大多數實驗室程序中扮演著重要角 色。作為一種用於液體處理的主要工具，移液管可定量地 抽吸及施配液體。有多種不同移液管設計滿足不同需求。 I又而&，會以多孔板搭配具有多個槽道的移液管使用以 便加速程序。當今在市面上有兩大類移液管。其中一類具 有一固定槽道間距而另一類具有一可調槽道間距，後者因 而有一較複雜機構。習知具有一固定槽道間距的移液管是 槽道間距為9 mm的8槽道型（參照圖丨和2)、槽道間距為9 mm的12槽道型、槽道間距為9㈤㈤的％槽道型（參照圖3)、 或槽道間距為4_5 mm的384槽道型。具有一可調槽道間距 的移液管是8槽道型和12槽道型，其可調間距不定但通常 在9 mm與20 mm之間。 113082.doc 200819198 具有一固定槽道間距之移液管一般係搭配具有相同或較 小間距之多孔板使用。舉例來說，具有固定9槽道間距 之12槽道型移液管適用於96孔的板及3料孔的板，但不適 用於具有一較大槽道間距的多孔板（譬如24孔的板）。具有 介於9瓜〇1與20 mm間之可調槽道間距的移液管可用在24 孔、96孔、384孔的板及俗稱之，，管對板轉移"的液體處 理，亦即若複數個直徑大於9mm之試管經適切配置，則可 利用一具有可調槽道間距的移液管抽吸此等試管内之液體 試樣隨後將此等液體試樣施配到譬如一 96孔的板中。此種 應用可有效地加速液體試樣之處理程序而不需要手動將液 體試樣從每一個別試管轉移到96孔的板之對應孔穴。 大體而言，具有越多移液管的液體處理器能夠同時處理 更多試樣且因此更有效率。如上文所述，具有一固定槽道 間距的習知移液管為8槽道型、12槽道型、96槽道型、或 384槽道型。圖2和2示出具有一 8槽道型液體處理移液管之 一習知液體處理器的示意圖。從圖中可見若要裝滿一習知 12x8陣列多孔板，液體處理器必須執行，，抽吸及施配，，動作 12次，而一具有12槽道型液體處理移液管的液體處理器必 須執行’’抽吸及施配”動作12次，二者均相當耗時。如上文 所述，8槽道型和12槽道型排列不適用於具有一較大槽道 間距的多孔板（譬如24孔的板）。又，如上文所述，具有一 可調槽道間距的移液管會有一較複雜機構，且因而較貴。 為克服以上所述既有液體處理器當中的缺點，今提出一 種多槽道液體處理移液管，其適用於不同組態的多孔板， 113082.doc 200819198 能夠相較於習知8槽道型和12槽道 之處理程序並執行”管對板轉移,，，且供^宜=試樣 的方式。 伢便且易於生產 【發明内容】 : 之-目標是提出—種多槽道液體處 適用於不同組_沾炙了丨化 從g v其 小"、，且恕的多孔板，能夠相較於習知 槽道型移液管加速液體試樣 m 移，，，且裎彳ϋ屈^日 ％序並執仃”管對板轉 _ 矛夕i美供一便宜易於生產的方式。 π 1本發明，該多槽道液體處理移液管具有—頂 部分。該w分設有複數個…該底部部： 5又有硬數個用於安裝管梢的移液頭。該等人Π每—者連接 至一注射筒或一且锯卞田 可硬接 署… 及施配液體之雙重功能的裝 之一相應孔穴。該多孔板應於一多孔板 列，八舒 夕孔板經配置成一 W、22x8或24χ16陣 鲁槽道間距，同時=移夜頁職、9麵及4·5職的 •來"… 亥4移液頭經配置成— 6X4陣列，在兩相 科、'文頭之間具有一 j 8 mm槽道間距。 本^明之其他目標、傷點芬主 、 丁权點及新穎特徵將在以下較佳實施 例评、、、田說明中以隨附圖式繪出。 【實施方式】 圖5疋 依據本發明之《一 &揭、音、放遍上 θ ^ 之夕槽道液體處理移液管的示意 圖。夕槽道液體處理移液管10具有一了頁部部分刚和一底 Τ部分·。頂部部分刚設有複數個入口（圖中隱藏）且底 以分2〇0没有用於安裝管梢搬的複數個移液頭2〇1。該 113082.doc 200819198 等入口（圖中隱藏)每-者連接至一注射筒（圖中隱藏）或一 具備定量抽吸及施配液體之雙重功能的裝置（圖中未示）並 與對應移液頭2CU連通。當已安裝於—自動化機械臂（圖中 未且被該臂或被手（s巾未示）㈣的多槽道液體處理移 液管1G接近-多孔板3叫’管梢搬每—者對應於多孔板 300之一相應孔穴（或行）30丨(參照圖7)。 多孔板300在兩相鄰孔穴3〇1之間有一槽道間距，同時移 • 液頭2〇1可被配置成一6x4陣列（參照圖5)且在兩相鄰移液頭 201之間有一18 mm的槽道間距。移液頭2〇ι更可被依一致 使移液頭之槽道間距除以多孔板3〇〇之槽道間距是二之冪 (例如2或4或類似數字）的方式配置。如[發明内容]中所 述，一習知6x4(24)陣列多孔板具有一 18爪瓜的槽道間距 (參照圖6)，一習知12x8(96)陣列多孔板具有一 9 mm的槽道 間距（參照圖7)，而一習知24x16(384)陣列多孔板具有一 4·5 mm的槽道間距（參照圖8)。因此，一可用於6χ4(24)、 春 12x8(96)或24x16(384)陣列多孔板之具有6χ4陣列的典型多 槽道液體處理移液管10可針對不同目的而有一18 mm(參照 圖6)、9 mm(參照圖7)或4·5 mm(參照圖8)槽道間距。由於 移液頭201之槽道間距對上第一槽道間距的比率會永遠保 持恆疋（一之冪），依據本發明的多槽道液體處理移液管10 有變通彈性且可輕易被修改適應於不同組態的多孔板，即 便習知多孔板之槽道間距針對新世代液體處理器作修改亦 如此。 圖4疋一正在進行’’管對板轉移”之一 6x4多槽道液體處理 113082.doc 200819198 移液官的示意圖。如上文所述，多槽道液體處理移液管 (圖中未示）係安裝於一自動化翁械臂（圖中未示）且被該臂 帶動’該臂通常被電腦程式控制。因此，多槽道液體處理 移液官可輕易地被控制和帶動以相對於多孔板3〇〇和—試 官架400水平地或鉛直地移動。從圖中可見多槽道液體處 理移液管可輕易地被控制和帶動以接近在試管架4〇〇中經 適切配置成一 6x4陣列且有一 18 mm中心間距的對應試管 401以抽吸該等試管内的液體，且隨後可被移動以接近並200819198 IX. Description of the Invention: [Technical Field] The present invention relates to a liquid processing pipette having a multi-channel design. [Prior Art] Modern laboratories, such as clinical and biological laboratories, often require a large number of simultaneous testing operations. Multiwell plates are widely used today to effectively handle such procedures. Conventional multiwell plates can have a configuration of 24 holes, 96 holes six or 384 holes and are configured in a rectangular configuration with a fixed center spacing (channel spacing). For example, a 24-well multiwell plate is configured as a 6x4 array with a channel spacing of 18 mm, a 96-well multi-well plate configured as a 12x8 array with a 9 mm channel spacing, and a 384-well multi-well plate. Configured as a 24" 16 array with a channel spacing of 4.5 mm. Liquid handling operations play an important role in most laboratory procedures. As a primary tool for liquid handling, pipettes can be used to pump and dispense liquid quantitatively. There are a variety of different pipette designs to meet different needs. I and & will use a multi-well plate with a pipette with multiple channels to speed up the process. There are two major types of pipettes on the market today. One of them has a fixed channel spacing and the other has an adjustable channel spacing, which in turn has a more complex mechanism. It is known that a pipette having a fixed channel spacing is an 8-channel type with a channel spacing of 9 mm (refer to Figures 丨 and 2), a 12-channel type with a channel spacing of 9 mm, and a channel spacing of 9 (five) (five). % channel type (refer to Figure 3), or 384 channel type with channel spacing of 4_5 mm. Pipettes with an adjustable channel spacing are 8-channel and 12-channel, with adjustable spacing but typically between 9 mm and 20 mm. 113082.doc 200819198 Pipettes with a fixed channel spacing are generally used with multi-well plates with the same or smaller spacing. For example, a 12-channel pipette with a fixed 9-channel spacing is suitable for 96-well plates and 3-well plates, but not for multi-well plates with a large channel spacing (eg 24 holes) board). Pipettes with adjustable channel spacing between 9 〇1 and 20 mm can be used in 24-well, 96-well, 384-well plates and commonly known as tube-to-plate transfer "liquid handling, ie If a plurality of tubes having a diameter greater than 9 mm are properly configured, a liquid sample in the tubes may be aspirated by a pipette having an adjustable channel spacing and then the liquid sample is dispensed to, for example, a 96-well. In the board. This application effectively accelerates the processing of liquid samples without the need to manually transfer liquid samples from each individual tube to the corresponding well of a 96-well plate. In general, a liquid handler with more pipettes is capable of handling more samples at the same time and is therefore more efficient. As described above, a conventional pipette having a fixed channel pitch is an 8-slot type, a 12-channel type, a 96-channel type, or a 384-channel type. Figures 2 and 2 show schematic views of a conventional liquid handler having an 8-channel liquid handling pipette. It can be seen from the figure that if a conventional 12x8 array perforated plate is to be filled, the liquid processor must perform, pump and dispense, act 12 times, and a liquid processor with a 12-channel liquid processing pipette The 'puffing and dispensing' action must be performed 12 times, both of which are quite time consuming. As mentioned above, the 8-slot and 12-slot arrangements are not suitable for multi-well plates with a large channel spacing ( For example, a 24-well plate.) As described above, a pipette having an adjustable channel spacing has a more complicated mechanism and is therefore more expensive. To overcome the disadvantages of the liquid processor described above, A multi-channel liquid handling pipette is now proposed which is suitable for multi-well plates with different configurations. 113082.doc 200819198 can perform "tube-to-board transfer" compared to the conventional 8-channel and 12-channel processing procedures. ,,, and the way to supply = sample. It is easy to produce and easy to produce [invention]: The goal is to propose a multi-channel liquid suitable for different groups _ 炙 炙 从 从 从 g g g g g g g g g g g g 多孔 多孔 多孔 多孔 多孔 多孔The conventional channel type pipette accelerates the liquid sample m, and the 裎彳ϋ ^ 日 % % % % % % % % % % % % % % % % According to the invention, the multi-channel liquid processing pipette has a top portion. The w portion is provided with a plurality of ... the bottom portion: 5 and a plurality of pipetting heads for mounting the pipe tips. Connected to a syringe or a sawhole, which can be hard-wired... and a corresponding hole for the dual function of the liquid. The perforated plate should be in a perforated plate, and the eight Shuxi plate is configured as a W, 22x8 or 24χ16 array Lu channel spacing, at the same time = shifting night page job, 9 face and 4·5 position • Come "... Hai 4 pipetting head is configured to - 6X4 array, in two phase, '文头There is a j 8 mm channel spacing between the other targets. The other targets, the injury points, the Ding Quan points and the novel features will be evaluated in the following preferred examples. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 5 is a schematic view of a liquid handling pipette of a channel in accordance with the present invention. The liquid channel pipette 10 has a page portion and a bottom portion. The top portion has just a plurality of inlets (hidden in the figure) and the bottom portion is divided into 2 〇 0. Multiple pipetting heads 2〇1. The 113082.doc 200819198 and other entrances (hidden in the figure) are connected to a syringe (hidden in the figure) or a device with dual functions of quantitative suction and dispensing of liquid (not shown) and connected to the corresponding pipetting head 2CU. When installed in an automated robotic arm (not shown in the figure and by the arm or by hand (snot shown) (four) liquid handling pipette The 1G proximity-perforated plate 3 is referred to as 'tube tip transfer each' corresponding to one of the corresponding holes (or rows) of the perforated plate 300 (see Fig. 7). The perforated plate 300 has a groove between two adjacent holes 3〇1 The track spacing, simultaneous shifting head 2〇1 can be configured as a 6x4 array (refer to Figure 5) and in two adjacent pipetting heads 201 There is a channel spacing of 18 mm. The pipetting head 2 〇 can be more consistently divided by the channel spacing of the pipetting head by the channel spacing of the perforated plate 3〇〇 is a power of two (eg 2 or 4 or similar) Mode configuration. As described in [Summary], a conventional 6x4 (24) array porous plate has a channel pitch of 18 claws (refer to FIG. 6), and a conventional 12x8 (96) array porous plate has A 9 mm channel spacing (see Figure 7), while a conventional 24x16 (384) array of perforated plates has a channel spacing of 4·5 mm (see Figure 8). Therefore, one can be used for 6χ4(24), A typical multi-channel liquid handling pipette 10 with a 6χ4 array of spring 12x8 (96) or 24x16 (384) array multiwell plates can have 18 mm (see Figure 6), 9 mm (see Figure 7) for different purposes or 4·5 mm (refer to Figure 8) channel spacing. Since the ratio of the channel spacing of the pipetting head 201 to the spacing of the upper first channel will always remain constant (one power), the multi-channel liquid processing pipette 10 according to the present invention has flexibility and can be easily modified. Adapted to perforated plates of different configurations, even though the channel spacing of conventional perforated plates is modified for new generation liquid processors. Figure 4疋1 is a 6x4 multi-channel liquid treatment of ''tube-to-board transfer' 113082.doc 200819198 Schematic diagram of the pipetting officer. As described above, multi-channel liquid processing pipette (not shown) It is mounted on an automated arm (not shown) and is driven by the arm. The arm is usually controlled by a computer program. Therefore, the multi-channel liquid handling pipetting officer can be easily controlled and driven to oppose the perforated plate. 3〇〇和—The trial frame 400 moves horizontally or vertically. It can be seen from the figure that the multi-channel liquid processing pipette can be easily controlled and driven to be close to the 6x4 array in the test tube rack 4 And a corresponding tube 401 having a center spacing of 18 mm to suck the liquid in the tubes, and then can be moved to approach and
施配液體給多孔板之對應孔穴3〇1(參照圖7)。此外，因為 多槽道液體處理移液管之18 111111槽道間距，該移液管可輕 >易地應付經適切配置且具有9_18 mm之一直徑的複數個^ 管。再者’由於多孔板300之行數（12)除以多槽道液體處理 移液管之行數（6)是2(2之冪），且多孔板·之列數（8)除以 多槽道液體處理移液管之列數（4)也是2(2之冪），故”管對 板轉移"之—完整循環可藉由2χ2(4)”抽吸及施配"程序完 成。相似原則因此可應用於—具有6 χ 4陣列或2 4 χ! 6陣列: 多孔板。亦可不㈣本發明之精神和範圍修改其 列樣式。 代^ 由以上說明可知’本發明提出—種用於一液體處理裳置 :多：道,接器’其有變通彈性且可輕易被修改適應於 同組…孔板及具有一較大直徑之試管，能夠相較於習 '8槽道型和12槽道型移液管加速液體試樣之處理程序， 且能克服習知技藝的缺點。儘管以 說明本發明，熟習此技藝者| ^ 乂锃貫施例 -者“里解到在隨附申請專利範圍 il3082.doc 200819198 項之精神及範圍以内，本發明仍可經修改實施。 【圖式簡單說明】 圖1是一位於_ 4办h 、 機械臂工作站之一習知液體處理器的示 意圖； 圖2是一且古一 、 : ”有一 8道移液排列之一習知多槽道液體處理移 液管的示意圖； 圖3是一呈古一 λ ^ 令一 12x8陣列移液排列之另一習知多槽道液 籲體處理移液管的示意圖； 圖4是一依據本發明執行”管對板轉移"之一 6x4多槽道液 體處理移液管的示意圖； 圖5是一圖4多槽道液體處理移液管之管梢接近一 12x8多 孔板之相應孔穴的局部放大示意圖； 圖6是一具有18 mm槽道間距之6x4多槽道液體處理移液 管之管梢接近具有18 mm槽道間距之一 6x4多孔板之相應孔The liquid is applied to the corresponding hole 3〇1 of the perforated plate (refer to Fig. 7). In addition, because of the 18 111111 channel spacing of the multi-channel liquid handling pipette, the pipette can be lightly > easily accommodated in a suitably configured configuration with a diameter of one of 9_18 mm. Furthermore, the number of rows (6) divided by the number of rows of the perforated plate 300 (12) divided by the multi-channel liquid processing pipette is 2 (power of 2), and the number of rows of the perforated plates (8) is divided by The number of rows of liquid handling pipettes (4) is also 2 (power of 2), so the "tube-to-board transfer"--complete cycle can be completed by 2χ2(4)" pumping and dispensing" . The principle of similarity can therefore be applied—with 6 χ 4 arrays or 2 4 χ! 6 arrays: multiwell plates. The column styles may also be modified without (4) the spirit and scope of the invention. From the above description, it can be seen that 'the present invention proposes a kind for a liquid treatment skirt: multi-channel, connector' which has flexible elasticity and can be easily modified to adapt to the same group... orifice plate and has a larger diameter The test tube can speed up the processing of the liquid sample compared to the '8 channel type and 12 channel type pipettes, and can overcome the shortcomings of the prior art. The present invention may be modified and implemented within the spirit and scope of the accompanying claims il3082.doc 200819198, to the extent that it is intended to be illustrative of the present invention. Brief Description of the Drawings Fig. 1 is a schematic diagram of a conventional liquid processor located at the _4 office, a robotic arm workstation; Fig. 2 is an ancient one: "" has a 8-channel pipetting arrangement of a conventional multi-channel liquid Schematic diagram of a pipette for processing a pipette; FIG. 3 is a schematic view of another conventional multi-channel liquid pipette pipetting pipe arranged in an array of ancient λ ^ and a 12x8 array; FIG. 4 is a diagram of performing a pipe according to the present invention; Schematic diagram of a 6x4 multi-channel liquid processing pipette for plate transfer; Figure 5 is a partial enlarged view of a corresponding hole of a multi-channel liquid processing pipette of a Figure 4 near a 12x8 perforated plate; 6 is a 6x4 multi-channel liquid handling pipette with a 18 mm channel spacing. The tube tip is close to the corresponding hole of a 6x4 perforated plate with a 18 mm channel spacing.
I 穴的示意圖； 馨 圖7是一圖4之6x4多槽道液體處理移液管之管梢接近具 有9 mm槽道間距之一 12x8多孔板之相應孔穴的示意圖；且 圖8是一 6x4多槽道液體處理移液管之管梢接近具有4 5 mm槽道間距之一 24x16多孔板之相應孔穴的示意圖。 【主要元件符號說明】 10 多槽道液體處理移液管 100 頂部部分 200 底部部分 201 移液頭 113082.doc -10 - 200819198 202 管梢 300 多孔板 301 孔穴 400 試管架 401 試管Schematic diagram of the I hole; Figure 7 is a schematic view of the tube tip of the 6x4 multi-channel liquid processing pipette of Figure 4 near the corresponding hole of a 12x8 perforated plate having a 9 mm channel spacing; and Figure 8 is a 6x4 The tube tip of the channel liquid handling pipette is close to the corresponding hole of a 24x16 perforated plate having a 4 5 mm channel spacing. [Main component symbol description] 10 multi-channel liquid processing pipette 100 Top part 200 Bottom part 201 Pipetting head 113082.doc -10 - 200819198 202 Tube tip 300 Perforated plate 301 Hole 400 Test tube rack 401 Test tube
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