TW200738886A - Methods and applications of molecular beacon imaging for infectious disease and cancer detection - Google Patents

Methods and applications of molecular beacon imaging for infectious disease and cancer detection

Info

Publication number
TW200738886A
TW200738886A TW095148836A TW95148836A TW200738886A TW 200738886 A TW200738886 A TW 200738886A TW 095148836 A TW095148836 A TW 095148836A TW 95148836 A TW95148836 A TW 95148836A TW 200738886 A TW200738886 A TW 200738886A
Authority
TW
Taiwan
Prior art keywords
molecular beacon
disease
specimen
marker
signal
Prior art date
Application number
TW095148836A
Other languages
Chinese (zh)
Inventor
Augustine Lin
Pan-Chyr Yang
Cheng-Chung Chou
Original Assignee
Alvitae Pharmaceuticals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alvitae Pharmaceuticals filed Critical Alvitae Pharmaceuticals
Publication of TW200738886A publication Critical patent/TW200738886A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Molecular beacon for detecting an infection and/or expression or a mutation of a disease marker for diagnostics and pharmacogenomics. The molecular beacon is capable of hybridizing a disease-related RNA or DNA of a disease marker in a specimen obtained from a living subject, thereby emitting a signal detectable without a need for signal amplification. The disease marker includes a genetic sequence specific to a pathogen including a flu virus, a cancer cell marker, and a drug resistance-related genetic mutation marker for a drug resistant cancer and infectious pathogen. To detect a disease cell, a specimen containing one or more cells is obtained from a living subject, and fixed by an organic solvent. A molecular beacon is then added to the specimen, followed by staining nuclei of the cells in the specimen. The signal is detectable with a microscope, FACS scan, ELISA plate reader, Scanner, or any combinations thereof.
TW095148836A 2005-12-23 2006-12-25 Methods and applications of molecular beacon imaging for infectious disease and cancer detection TW200738886A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75365105P 2005-12-23 2005-12-23
US75396005P 2005-12-23 2005-12-23

Publications (1)

Publication Number Publication Date
TW200738886A true TW200738886A (en) 2007-10-16

Family

ID=38218631

Family Applications (2)

Application Number Title Priority Date Filing Date
TW095148836A TW200738886A (en) 2005-12-23 2006-12-25 Methods and applications of molecular beacon imaging for infectious disease and cancer detection
TW095148837A TW200745345A (en) 2005-12-23 2006-12-25 Methods and applications of molecular beacon imaging for identifying and validating genomic targets, and for drug screening

Family Applications After (1)

Application Number Title Priority Date Filing Date
TW095148837A TW200745345A (en) 2005-12-23 2006-12-25 Methods and applications of molecular beacon imaging for identifying and validating genomic targets, and for drug screening

Country Status (4)

Country Link
US (2) US20080274450A1 (en)
EP (2) EP1977006A2 (en)
TW (2) TW200738886A (en)
WO (2) WO2007075929A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI618932B (en) * 2012-03-08 2018-03-21 上海新申派科技有限公司 Micro-devices for improved disease detection
TWI630385B (en) * 2012-07-16 2018-07-21 昌和生物醫學科技（揚州）有限公司 Devices and methods for enhanced detection and identification of diseases

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838302B2 (en) * 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
EP2186827A1 (en) 2008-11-14 2010-05-19 HS LifeSciences Ltd. Surrogate marker directed cDNA cloning of selectively induced mRNAs
US20160177396A1 (en) * 2014-12-22 2016-06-23 Enzo Biochem, Inc. Comprehensive and comparative flow cytometry-based methods for identifying the state of a biological system
JP7017193B2 (en) * 2019-08-23 2022-02-08 国立大学法人東海国立大学機構 RNA action inhibitor and its use
CN114277137B (en) * 2020-03-30 2022-09-02 中国医学科学院肿瘤医院 Application of exosomes ARPC5, HMGN1 and the like in lung cancer diagnosis

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1025120B1 (en) * 1997-10-27 2010-08-18 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6500650B1 (en) * 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
EP1172445A1 (en) * 2000-07-14 2002-01-16 Praenadia GmbH A method for direct genetic analysis of target cells by using fluorescence probes
AU2001244577A1 (en) * 2000-09-29 2002-04-15 Bml, Inc. Method of detecting norwalk-like virus (gi)
KR20050100371A (en) * 2003-01-13 2005-10-18 에모리 유니버시티 Methods of detecting gene expression in normal and cancerous cells
JP4397659B2 (en) * 2003-09-11 2010-01-13 独立行政法人科学技術振興機構 DNA detection method using molecular beacon using switching between monomer emission and excimer emission of fluorescent molecules
WO2006060561A2 (en) * 2004-12-01 2006-06-08 Emory University Methods and applications of molecular beacon imaging for cancer cell detection

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI618932B (en) * 2012-03-08 2018-03-21 上海新申派科技有限公司 Micro-devices for improved disease detection
TWI630385B (en) * 2012-07-16 2018-07-21 昌和生物醫學科技（揚州）有限公司 Devices and methods for enhanced detection and identification of diseases
TWI711819B (en) * 2012-07-16 2020-12-01 大陸商昌和生物醫學科技（揚州）有限公司 Devices and methods for enhanced detection and identification of diseases

Also Published As

Publication number Publication date
TW200745345A (en) 2007-12-16
EP1979491A2 (en) 2008-10-15
EP1977006A2 (en) 2008-10-08
US20080274450A1 (en) 2008-11-06
WO2007075924A9 (en) 2008-06-26
WO2007075929A3 (en) 2008-10-23
US20080032282A1 (en) 2008-02-07
WO2007075929A2 (en) 2007-07-05
WO2007075924A2 (en) 2007-07-05
WO2007075924A3 (en) 2008-08-14

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