TW200718933A - Staining solution of horny layer cells and method for staining horny layer cells using it - Google Patents

Staining solution of horny layer cells and method for staining horny layer cells using it

Info

Publication number
TW200718933A
TW200718933A TW095132645A TW95132645A TW200718933A TW 200718933 A TW200718933 A TW 200718933A TW 095132645 A TW095132645 A TW 095132645A TW 95132645 A TW95132645 A TW 95132645A TW 200718933 A TW200718933 A TW 200718933A
Authority
TW
Taiwan
Prior art keywords
staining
horny layer
layer cells
alcohol
solution
Prior art date
Application number
TW095132645A
Other languages
Chinese (zh)
Inventor
Kazuhiro Yamazaki
Norio Fujiwara
Tadahito Seto
pei-ming Yang
Original Assignee
Pola Chem Ind Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pola Chem Ind Inc filed Critical Pola Chem Ind Inc
Publication of TW200718933A publication Critical patent/TW200718933A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements

Abstract

The purpose of the present invention provides a technique for staining horny layer cells, which stains horny layer cells on the instant and distinctly, and prevents spots of staining. Namely, in order to maintain good staining as desired and decrease the volatile speed of alcohol, to produce a staining solution comprising (1) dye, (2) 10~50% by mass of alcohol with 1~4 carbon atoms, (3) aromatic alcohol and (4) organic acid, used for staining. According to usage of staining solution of the present invention, it can stain horny layer cells simply, distinctly and with evenness.
TW095132645A 2005-09-06 2006-09-05 Staining solution of horny layer cells and method for staining horny layer cells using it TW200718933A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2005257451 2005-09-06

Publications (1)

Publication Number Publication Date
TW200718933A true TW200718933A (en) 2007-05-16

Family

ID=37835562

Family Applications (1)

Application Number Title Priority Date Filing Date
TW095132645A TW200718933A (en) 2005-09-06 2006-09-05 Staining solution of horny layer cells and method for staining horny layer cells using it

Country Status (3)

Country Link
JP (1) JPWO2007029437A1 (en)
TW (1) TW200718933A (en)
WO (1) WO2007029437A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5162738B2 (en) * 2008-06-18 2013-03-13 学校法人東京医科大学 Methods for isolating and collecting living cells and structures in living cells
JP5491228B2 (en) * 2010-02-16 2014-05-14 御木本製薬株式会社 Staining solution and staining method
US20130203109A1 (en) * 2012-01-26 2013-08-08 Leica Biosystems Richmond, Inc. Methods and Compositions for Hematoxylin and Eosin Staining
CN115372109A (en) * 2022-10-24 2022-11-22 北京汉氏联合生物技术股份有限公司 Circulating tumor cell staining kit

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3928554A (en) * 1973-12-19 1975-12-23 Block Engineering Emulsion dyeing
JPS6047960A (en) * 1983-08-26 1985-03-15 Wako Pure Chem Ind Ltd Staining method and staining test solution of cell in cytology
DE4432854C1 (en) * 1994-09-15 1996-03-07 Kao Corp Gmbh Procedure for examining the degree of damage to human hair
DE10024086C1 (en) * 2000-05-18 2001-09-27 Merck Patent Gmbh Phenol-free bacterial stains, useful e.g. for identifying acid-fast mycobacteria, includes e.g. benzyl alcohol as safe alternative for phenol
JP3618093B2 (en) * 2001-10-29 2005-02-09 ポーラ化成工業株式会社 Keratinocyte staining method and keratinocyte preparation method
JP2005106795A (en) * 2003-10-01 2005-04-21 L'oreal Sa Evaluation or diagnostic kit
JP2006053117A (en) * 2004-07-12 2006-02-23 Pola Chem Ind Inc Liquid for dyeing corneocyte and method for dyeing corneocyte using it

Also Published As

Publication number Publication date
WO2007029437A1 (en) 2007-03-15
JPWO2007029437A1 (en) 2009-03-12

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