TW200520825A - Process of extracting Gastrodiae Rhizoma by super-critical fluid - Google Patents

Abstract

This invention provides a process for extracting and refining Gastrodiae Rhizoma, which comprises techniques of grinding, sieving, packing, extracting, discharging, sampling and analyzing.

Description

200520825 发明 Description of the invention: [Technical field to which the invention belongs] The present invention relates to a process technology for extracting and refining the Chinese herbal medicine ingredient, Gastrodia elata. This technology uses grinding, screening, filling, extraction, unloading, sampling, analysis The processing procedure is the technology of extracting Gastrodia elata. [Previous Technology] Extracts of Chinese herbal medicines often contain a large amount of interfering substances, such as chlorophyll, wax, or other substances, thus causing the disadvantage of impure product components and reducing its use value. [Summary of the invention] The supercritical fluid extraction used in this program is mainly composed of 4 parts: a liquid extraction solvent packed in a high-pressure rigid bottle; a high-pressure pump: the liquid extraction solvent is pumped into a high-pressure drum device, and the co-solvent is mixed at the same time to The isokinetic method and the extraction solvent are sent to the extraction tank; the extraction device: the tank is heated by a heating belt method to preheat the extraction solvent to a supercritical state, and enters the extraction tank to contact the gastrodia powder for extraction; the absorption tank: via After the pressure is reduced, the extraction solvent is separated from the extract, and the extract is absorbed into the solution by an appropriate absorbent. [Embodiment] Gastrodia leaves are firstly ground into a high-speed grinder, and then poured into a stainless steel mesh with a size of 140rnesh ((u〇5mm) mesh and sieved. Cut 5 to 6 cm long glass wool plugs Into the bottom of the extraction tank, put about 90 milligrams of powder of Gastrodia elata into the high-pressure extraction tank, and then put ethanol into the first and closing tanks as the absorbent and the absorption solvent of the extract. When sampling later, you must First close the pump and the needle valve, and open the communication valve to allow the liquid level in the window separation tank to rise and stabilize, then open the sampling valve and take a sampling bottle to sample. The purified gastrodia elution solution is purified. First, 〇45 // m nylon membrane filter paper to remove large particles and insoluble materials. This filter was removed by vacuum drying method and then the dry matter was dissolved in a methanol solution of%. This solution was passed through and 200520825.

The solid-phase extraction cassette composed of Cl8 was discarded, and then the lactone adsorbed in the C18 extraction cassette was washed out with a 400/0 methanol solution, and the eluate was directly placed in the solvent to complete the process. μ by removing [Schematic description of the diagram] Pick up and apply for patent scope: 1. A process technology for extracting and purifying Gastrodia elata, including: Zhishuo Faqing uses the equipment diagram shown in Figure 1 to cooperate with the implementation method described on page 5. 'Make clean products.

Claims (1)

200520825 Cl8 solid phase extraction cassette, discard the polyamide extraction cassette, and then use a 400/0 methanol solution to wash out the lactone adsorbed in the C18 extraction cassette, this eluate is directly in the solvent, which is complete. μ by removing [Schematic description of the diagram] Pick up and apply for patent scope: 1. A process technology for extracting and purifying Gastrodia elata, including: Zhishuo Faqing uses the equipment diagram shown in Figure 1 to cooperate with the implementation method described on page 5. 'Make clean products. 200520825 (1) Designated representative map: (1) The designated representative map in this case is: (). (2) Brief description of the element representative symbols of this representative map: 捌 If there is a chemical formula in this case, please disclose the chemical formula that can best show the characteristics of the invention: