TW200517499A - Agent for maintaining embryonic stem cells in undifferentiated state - Google Patents

Agent for maintaining embryonic stem cells in undifferentiated state

Info

Publication number
TW200517499A
TW200517499A TW093123544A TW93123544A TW200517499A TW 200517499 A TW200517499 A TW 200517499A TW 093123544 A TW093123544 A TW 093123544A TW 93123544 A TW93123544 A TW 93123544A TW 200517499 A TW200517499 A TW 200517499A
Authority
TW
Taiwan
Prior art keywords
stem cells
embryonic stem
agent
undifferentiated state
maintaining
Prior art date
Application number
TW093123544A
Other languages
Chinese (zh)
Inventor
Ryuichi Nishinakamura
Takuya Sugiyama
Original Assignee
Tanabe Seiyaku Co
Univ Tokyo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tanabe Seiyaku Co, Univ Tokyo filed Critical Tanabe Seiyaku Co
Publication of TW200517499A publication Critical patent/TW200517499A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

This invention relates to an agent for maintaining embryonic stem cells in the undifferentiated state which contains a protein belonging to the Wnt family as the active ingredient; a medium for maintaining embryonic stem cells in the undifferentiated state which contains the above agent; a process for producing embryonic stem cells characterized by comprising culturing inner cell mass cells obtained from a mammalian embryo at the blastocyst stage in the presence of the above agent to thereby give embryonic stem cells; and a method of maintaining embryonic stems cell in the undifferentiated state characterized by comprising maintaining established embryonic stem cells in the presence of the above agent.
TW093123544A 2003-08-05 2004-08-05 Agent for maintaining embryonic stem cells in undifferentiated state TW200517499A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003287207A JP2006345702A (en) 2003-08-05 2003-08-05 Agent for maintaining embryonic stem cell in undifferentiated state

Publications (1)

Publication Number Publication Date
TW200517499A true TW200517499A (en) 2005-06-01

Family

ID=34114008

Family Applications (1)

Application Number Title Priority Date Filing Date
TW093123544A TW200517499A (en) 2003-08-05 2004-08-05 Agent for maintaining embryonic stem cells in undifferentiated state

Country Status (3)

Country Link
JP (1) JP2006345702A (en)
TW (1) TW200517499A (en)
WO (1) WO2005012511A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2463476T3 (en) 2004-10-19 2014-05-28 Regeneron Pharmaceuticals, Inc. Method to generate a homozygous mouse for a genetic modification
EP2692860A4 (en) 2011-03-31 2015-05-20 Riken Regulator of anaplastic state and applications therefor
US10550364B2 (en) 2014-12-24 2020-02-04 Osaka University Method for producing Wnt protein and method for storing Wnt protein

Also Published As

Publication number Publication date
WO2005012511A1 (en) 2005-02-10
JP2006345702A (en) 2006-12-28

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