TW200505937A - Methods and compositions for identifying modulators of g protein-coupled receptors - Google Patents

Methods and compositions for identifying modulators of g protein-coupled receptors

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Publication number
TW200505937A
TW200505937A TW093112289A TW93112289A TW200505937A TW 200505937 A TW200505937 A TW 200505937A TW 093112289 A TW093112289 A TW 093112289A TW 93112289 A TW93112289 A TW 93112289A TW 200505937 A TW200505937 A TW 200505937A
Authority
TW
Taiwan
Prior art keywords
gpcr
ligand
upregulatable
coupled receptors
modulators
Prior art date
Application number
TW093112289A
Other languages
Chinese (zh)
Other versions
TWI347949B (en
Inventor
Fu-Yue Zeng
Dominic P Behan
Original Assignee
Arena Pharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arena Pharm Inc filed Critical Arena Pharm Inc
Publication of TW200505937A publication Critical patent/TW200505937A/en
Application granted granted Critical
Publication of TWI347949B publication Critical patent/TWI347949B/en

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  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The subject invention provides methods for making ligand upregulatable G-protein coupled receptors (GPCRs). Ligand upregulatable GPCRs contain a modified TM5-IC3-TM6 segment, which provides for increased levels of the ligand upregulatable GPCR I a host cell in the presence of a ligand as compared to the absence of the ligand. The subject invention also provides assays for screening test compounds for a ligand of a GPCR using a ligand upregulatable GPCR. In these assays, a ligand upregulatable GPCR is contacted with a test compound, and an increase in the detectable amount of the ligand upregulatable GPCR in the host cell indicates that the test compound is a ligand of the GPCR. Said indicated ligands encompass modulators of the GPCR. Said modulators of the GPCR are particularly useful in treating GPCR-related conditions.
TW093112289A 2003-04-30 2004-04-30 Methods and compositions for identifying modulators of g protein-coupled receptors TWI347949B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46748903P 2003-04-30 2003-04-30
US51616803P 2003-10-31 2003-10-31

Publications (2)

Publication Number Publication Date
TW200505937A true TW200505937A (en) 2005-02-16
TWI347949B TWI347949B (en) 2011-09-01

Family

ID=46765590

Family Applications (3)

Application Number Title Priority Date Filing Date
TW093112289A TWI347949B (en) 2003-04-30 2004-04-30 Methods and compositions for identifying modulators of g protein-coupled receptors
TW100118780A TW201131168A (en) 2003-04-30 2004-04-30 Methods and compositions for identifying modulators of G protein-coupled receptors
TW100122527A TW201144436A (en) 2003-04-30 2004-04-30 Methods and compositions for identifying modulators of G protein-coupled receptors

Family Applications After (2)

Application Number Title Priority Date Filing Date
TW100118780A TW201131168A (en) 2003-04-30 2004-04-30 Methods and compositions for identifying modulators of G protein-coupled receptors
TW100122527A TW201144436A (en) 2003-04-30 2004-04-30 Methods and compositions for identifying modulators of G protein-coupled receptors

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TW (3) TWI347949B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7297214B2 (en) * 2017-09-28 2023-06-26 国立大学法人京都大学 Fish and methods of producing fish

Also Published As

Publication number Publication date
TW201131168A (en) 2011-09-16
TWI347949B (en) 2011-09-01
TW201144436A (en) 2011-12-16

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