200417311 玖、發明說明（1) 【發明所屬之技術領域】 本發明是提供-種動物行為評估方法及其設備，特別 是指一種動物興致程度評估方法及其設備。 【先前技術】200417311 (1) [Explanation of the invention] [Technical field to which the invention belongs] The present invention provides a method for assessing animal behavior and a device thereof, and particularly relates to a method and apparatus for evaluating an animal's interest. [Prior art]
5 (Sucrose preference test)^ * Willner P 等人於1987年研發出來用於量測老鼠興致㈣〇r hedonia)，或是“憂鬱程度，，及“抗憂營劑療效反應，，的方法 〇 如第一圖所示，上述蔗糖偏好試驗是預先將一老鼠 91收谷於-實驗嘴I 92巾，並且24小時不給喝水。然後 在該實驗籠92内擺設-裝有清水之清水瓶％及―與該清 尺瓶93等同並裝有2-3%蔗糖水（sucr〇se)之糖水瓶94，並 且於半小時後將該清水瓶93與該糖水瓶94之位置互換， 再、、、i過半小時後取下該清水瓶93與該糖水瓶％，測量其 15巾所含清與蔗糖水之剩餘量，以得知在上述—小時内該 老鼠91所消耗之清水與蔗糖水的量。 依據過去的研究結果顯示，一般老鼠91先天就偏好 具有甜味的糖水，因此在一般狀態下，該蔗糖水的消耗量 會高於該清水的消耗量，而根據Willner的研究，若將該 2〇 老乳91對該蔗糖水的喜好當作一種興致（interest)，而受 壓力或憂鬱的時候會喪失興致，則將該老鼠91暴露於，，壓 力’’下，或使其產生，，憂鬱，，的狀態時，該老鼠91對於偏好 之蔗糖水的興致將會降低，而使得其對於該蔗糖水的攝取 比率降低。而若將一有效的抗憂營藥劑注射於該老鼠91 200417311 玖、發明說明（2) 體内後，因為興致回升，使其對於偏好之蔗糖水的興致恢 復，因此，其攝取蔗糖水之比率也將恢復至先前所述的一 般狀態。 但是依 Naida F· Forbes 等人於 Physiology & behavior， 5 Vol· 60·Ν〇· 6, ρρ·1481-1484，1996 所發表之”Chronic Mild Stress and Sucrose Consumption : Validity as a Model of Depression”一文，指出其等同樣依上述蔗糖偏好試驗進行 實驗卻無法得出相同之結果。特別是將該蔗糖水消耗量除 以該老鼠91體重進行修正後，所得到該老鼠91每單位體 10 重消耗的蔗糖水量並沒有明顯變化，因此認為上述蔗糖偏 好試驗將該裝有清水之清水瓶93與該裝有蔗糖水之糖水 瓶94放置於平行位置時，僅能測量該老鼠91對該蔗糖水 的喜好或需求。例如該老鼠91飢餓時可能會攝取較多之 蔗糖水以補充能量，而口渴時可能會飲用較多能解渴的清 15 水，但無法支持或證明該蔗糖水之消耗量與該老鼠91的 興致或憂鬱有關，當然也就無法驗證施加壓力之效果，以 及注射抗憂鬱藥劑的療效。 【發明内容】 因此，本發明之主要目的是在提供一種具有的動物興 20 致程度評估方法及其設備。 本發明之另一目的是在提供一種能區隔動物喜好與興 致的動物行為評估方法及其設備。 於是，本發明一種動物興致程度評估方法，包括下列 步驟： 6 200417311 玖、發明說明（3) &)將一動物限制於一活動空間； b)提供該動物於該活動空間内可取得飲用之一清水 ’以及該動物較喜好飲用之—偏好液體，且該偏 好液體取得位置高於該清水取得位置； 5 e)量測該動物於—預定期間⑽用該清水與偏好液 體量；及 d)比較該動物飲用該清水與偏好液體量，以i評估 該動物於該預定期間内之興致程度。 而依上述方法所製成之動物興致程度評估設備則包括 1〇 -箱體、-設置於該箱體上之清水容器，以及—設置於該 箱體上之偏好液體容器。該箱體具有一底座及一由該底座 向上延伸之圍繞壁，該底座與該圍繞壁能界定出一活動空 間。該清水容器具有-與該活動空間相連通並鄰近該底^ 之清水出口，而該偏好液體容器則具有一與該活動空間相 15連通且位置高於該清水出口之偏好液體出口。 【實施方式】 本發明之前述以及其他技術内容、特點與優點，在以 下配合參考圖式之-較佳實施例的詳細說明中，將可清楚 的明白。 2〇 衫意的是，本發明動物興致程度評估方法之較佳實 施例所採用的試驗動物為十隻C57BL/6黑色小鼠，然而 並非以此為限，例如一般實驗也常使用的BALB/c小鼠， 甚至於其他動物亦均能應用本發明進行興致程度評估。在 本實施例中，該等黑色小鼠之平均體重約為23公克至乃 200417311 玖、發明說明（4) 公克，且較為活躍 主要是夜間的個二是消耗水分較多的時間， 僅供應清水時，如第經實驗三日所得結果證明，若 晚上12小時内戶斤需 句於~彳目 5 10 15 岍而之飲水量約為4.0 cc至45 π ^ 供給清水與蔗糖水， 。右同時 放置，如第三圖所亍t 以相等之高度平行 〇·5 cc至1 cc的清水盥 、曰攝取約 一 ·5 ^至6_〇 cc的蔗糖水， 黑色小w對蔗糖水的喜好—Τ見該專 糖水的困難度提高 '^ 7而右將取得該蔗 又徒回，例如將該蔗糖水放置於 使該等黑色小鼠必須付出 、回之位置， 如第四Η所-w 方能取得薦糖水時， =四圖所不,等黑色小鼠—天將 1.5CC的清水與3·5 He至 薦糖水不是該等黑色小鼠生γ^γ’可見由於上述的 命與生存，更不會增加二:去:用，並且不會妨礙其生 ^ ^ ^痛古。因此當該等黑色小鼠願竜 化費額外的勞力或麻煩去 ' 取用¥，便能顯示出其興致。 因此’本發明即是以_動物能力範圍内能取得斧 糖水之偏好食品及一如清次夕淡4人 抵 月水之維生食品，在該動物能自由 選擇的情況下’藉由增加該偏好食品取得過程之困難度， 而達到提升鑑別興致或偏好的能力。 如第五圖至第七圖所示，本發明動物興致程度評估方 法之較佳實施例包括下列步驟·· 本實施财，該受評估之動物1為上狀黑色小鼠 步驟_，將一動物i限制於一活動空間2〇内。在 tM U.l JU JO- 、〇 上·《 · · 而1 20 200417311 玖、發明說明（5) 活動空間20則是囍由_ γβ _ 幻疋糟甶相體2所界定。該箱體2具有一 底座21 &由4底座21向上延伸之圍繞壁該底座 1…玄圍、、凡壁22月匕界定出限制動物i活動之該活動空間 20 〇 步驟102，提供動物！於該活動空間2〇内可取得飲 用之π水在此疋將盛裝有供動物1維生之清水的一清 水容器3設置於該箱體2上，該清水容器3具有一中空之 外套筒31以及彳设於該外套筒3 i内之活塞η，該活 塞32與該外套筒31密合並形成一盛裝清水之清水容室 〇 310該外套筒31上更形成有一與該活動空間2〇相連通 並鄰近該底座21之清水出口 3〇，動物i便是藉由該清水 出口 30攝取清水飲用。 步驟104，提供動物！於該活動空間2〇内取得位置 间於4 π水取得位置，且為動物丨較喜好飲用之一偏好液 15體。在本實施例中，該偏好液體是蔗糖水，當然並不以此 為限，該偏好液體也可以是可樂或含酒精之飲料。 同樣地，該偏好液體是盛裝於一偏好液體容器4内， 而該偏好液體容器4也是設置於該箱體2上，且具有一中 空之外套筒41以及一穿設於該外套筒41内之活塞42， 20 °亥活塞42與該外套筒41密合並形成一偏好液體容室4 i 〇 。該外套筒41上更形成有一與該活動空間2〇相連通並遠 離該底座21之該偏好液體出口 4〇，使得該偏好液體出口 40之位置高於該清水出口 3〇之位置。該箱體2更具有一 設置於該圍繞壁22上之管梯5，該管梯5具有一中空且 200417311 玖、發明說明（6) 如蛇腹呈圓弧狀連續起伏之管體52，一形成於該管體52 内之通道520’以及兩位於該管體52兩相反端之端部51 、51’。該等端部51、51，其中之一端部51，鄰近該偏好液 體出口 40並與該偏好液體容器4相連接，而該等端部51 5 、其中另一端部51則鄰近該底座21。動物1能藉由該 管梯5之通道520接近該偏好液體容器4並由該偏好液體 出口 40取得該偏好液體飲用。 步驟106，量測動物1於一預定期間内飲用該清水與 該偏好液體量。由於該清水容器3之活塞32上裝設有一 10 用於量測該清水於該清水容室310内之液面高度的感測器 61，而該偏好液體容器4之活塞42也裝設有一用於量測 該偏好液體於該偏好液體容室41〇内之液面高度的感測器 61，在本實施例中，各該感測器61、61 ’為一超音波收發 器，且該等感測器61、01，更與一超音波儀（圖未示）相連 15 接，並能精確且自動地紀錄該清水於該偏好液體之消耗量 〇 該預定期間在本實施例中為一日，當然，該預定期間 可隨實驗需要而設計不同之週期，並不以一日為限。經實 驗結果發現，動物1於一般狀態下，如第四圖所示，於該 20預定期間内會消耗約1·〇 CC至1.5 CC的清水與3.5 CC至4_0 cc的偏好液體。相對於如第三圖所示相等高度平行放置之 /月水約〇·5 cc至1 cc及偏好液體蔗糖水5.5 cc至0.0 cc的 消耗量，顯示出架高後之該偏好液體容器4的確能增加動 物1取得該偏好液體的困難度。 10 玖、發明說明（7) 另 ^一 —級有關壓力能否降低動物1飲用偏好液體興致的 實驗，如第同 — 曰 圖所不，動物1於未受壓力時，偏好液體消 耗高於清水消耗量，且平均差距約為2 cc ;而於受壓 ft下時’如第九圖所示，動物1之偏好液體消耗量清 5水/肖耗里則並非相同，其中編號2、編號3、編號5、編 说6編就8及編號10之黑色小鼠仍維持偏好液體消耗 置南於清水消耗量之情況，但編號丨、編號4、編號7及 編唬9之黑色小鼠的偏好液體消耗量均明顯低於清水消耗 量。 10 步驟108，量測動物1於該預定期間内之活動量。如 第六圖及第十圖所示，該箱體2側邊裝設一與該活動空間 20相連通並鄰近該底座21之運動裝置7。該運動裝置7 具有一樞接於該圍繞壁22上供上述黑色小鼠使用之滾輪 71。該箱體2與該滾輪71上更裝設有一計數裝置8，該 15 計數裝置8具有至少一設置於該滾輪71上之反光片81、 一設置於該圍繞壁22上並向該滾輪71方向發射光線之光 源82、一設置於該圍繞壁22上並能接收由該反光片81 反射該光源82所產生之光線的接收器83，以及一與該接 收器83連接之計數器84。因為該滾輪71並不會反射由 20 該光源82所發射之光線，因此該滾輪71於一圈的滾動範 圍内，會轉動至一特定位置，使該接收器83正好接收到 一由該反光片81反射之光訊號，此時該計數器84便會記 錄該滾輪71滾動一圈。 由於量測動物1於該預定期間内跑步圈數即能計算出 11 200417311 玖、發明說明（8) 動物1之活動量。故直接由該計數器84計算該滾輪71於 該預定時間内滾動之圈數，即能推估出該動物之健康狀況 。如第十一圖所示，於進行上述實驗控制變因為施加使動 物1興致降低之壓力時，該等受測黑色小鼠平均一天約跑 5 15000圈至20000圈滾輪71。 步驟110，比較動物i飲用該清水與偏好液體量，以 評估動物i於該預定期間内之興致程度。依上述實驗步驟 所獲得之結果發現，動物i在一般狀態下，如第八圖所示 ’動物1之偏好液體消耗量皆約高於清水消耗量2cc;而 H)在受壓力狀態下時，如第九圖所示，動物i之偏好液體消 耗量清水消耗量則並非相同，其中編號i、編號4、編號 7及編破9之黑色小鼠的偏好液體消耗量明顯地低於清水 消耗量，而編號2、編號3、編號5、編號6、、編號8及編 號一 1〇之黑色小鼠則似乎未受施加之壓力影響，很清楚地 顯示出每一隻黑色小鼠受到相同麼力後之反應不盡相同。 v驟112’依動物丨於該預定期間内之活動量校正動 物1於該預定期間内之興致程度。由於取得該偏好液體之 困難度較高’必須付出額外之勞動力方能取得，因此必須 監測動物1之健康狀況，是否仍符合原先之設定件，使得 該偏好液體之取得條件仍維持於動物！之能力範圍内。依 據步驟108中所得之動物】 動物1於该預定期間内跑滾輪71之 圈數，便能修正步驟U〇中所推估之興致程度，並確認該 偏好㈣出口 40仍位於動物i取得之能力範圍内，以確 吞忍所得實驗結果有效。太眚 本實知例是將跑滾輪71 ®數低於 12 200417311 玖、發明說明（9) 3〇〇〇圈之實驗數據剔除，如第十—圖所示，無論施加壓 力與否，在實驗過程中，並沒有任何一隻黑色小鼠跑滾輪 71圈數低於3000圈。 步驟114，完成動物丨興致程度之評估。 5 在上述動物興致程度評估方法中，步驟110以動物i ㈣該清水與偏好液體消耗量評估動物i之興致程度，如 第九圖所不，其結果發現在受壓力狀態下時，動物i之偏 好液體消耗里清水消耗量則並非相同，明確地顯示出受到 相同壓力後，每-隻黑色小鼠之興致產生不同程度的變化 1〇 ’且並非所有黑色小鼠都會降低興致，顯現出本發明能區 刀出以往傳統方法所無法區隔出之個體差異。 ^此外，步驟102與步驟104所提供清水與偏好液體， 月b以不同之維生食品以及偏好食品進行替代，但該維生食 品與該偏好食品必須能供受測動物丨明顯地分辨出喜惡。 田；、、；考畺實施之可行性，相對於該盛裝清水之清水容器 3及該盛裝偏好液體之偏好液體容器4，也應分別以適合 收納維生食品的維生食品容器，以及適合收納偏好食品的 偏好食品容器取代。 而上述步驟106中，使用該等感測器61、01，是為了 〇旎達到連續並自動化監測的功效，但並不以此為限，由於 步驟106是為了量測該清水及該偏好液體之消耗量，因此 右改以重量進行量測、甚至於以人力讀取容量刻度等，亦 能達到相同之目的。 另外’由於將該偏好液體容器4之偏好液體出口 4〇 13 200417311 玖、發明說明（10) 設置於該管梯5遠離該 出口 30之設置位署甘 之~51，以面於該清水 體之困難㈣/ 的在於使動物1取得該偏好液 、隹加，以區隔出動物1之興致與喜好的差里 f而提升鐘別興致或«的能力。故熟習該項技藝人= Γ輕易推想，凡是只要能增加動物1接近或取得該偏好: :之過程相對於該維生食品較為困難，使得動物i : 費額外的勞力方能取得該偏好食品，例如斜坡，二 ΐ雜開關等方法，均同樣能達到相同功效，而為本發明; 範轉。 < 在此必須說明的是，若使動物1於取得該偏好液體之 過程裝會遭受痛苦，例如於該管梯5上裝設—電擊裝置（ 圖未示），而動物丨仍願意忍受痛苦而進行取用時，則表 示動物1對該缺液體已經錢，有了依賴性，其不飲^ 之痛苦將大於受電擊之痛苦，财±述興致所造成之行為 。當動物1出現上述成瘾現象時’便表示其實驗結果非關 興致之行為表現，因此亦能做為本發明動物興致評估方法 之驗證。 依據上述之評估方法可知，本發明動物興致程度評估 設備包括該箱體2、該清水容器3、該偏好液體容器4、 20 該量測裝置6、該運動裝置7，以及該計數裝置8。 該箱體2具有該底座21、該由底座21向上延伸之圍 繞壁22，以及該設置於圍繞壁22上之管梯。該箱體2形 成有该由底座21與圍繞壁22界定出並供動物1活動之活 動空間20。该清水谷态3設置於該箱體2上，並具有該 14 200417311 玖、發明說明（11) 中二之外套同3 1、该穿設於該外套筒3 1内之活塞3 2、該 由活塞32與外套筒31所界定之清水容室31〇，以及該形 成於外套筒31上並與活動空間2〇相連通之清水出口 ％ 〇 忒偏好液體容器4設置於該箱體2上，並具有該中空 之外套筒41、該穿設於該外套筒41内之活塞42、該由活 塞42與外套筒41界定之偏好液體容室41〇，以及該形成 於外套筒41上並同樣與活動空間2〇相連通之偏好液體出 口 40。該偏好液體出口 4〇之位置高於該清水出口 3〇之 1〇 位置。該管梯5具有該中空之管體52，該形成於管體52 内之通道520、該位於管體52上並鄰近該偏好液體出口 40之端部51，，以及該位於管體52上相反於該端部51，處 且鄰近該底座21之端部51。 该量測裝置6具有該裝設於該清水容器3之活塞32 15 上之感測器61、該裝設於該偏好液體容器4之活塞42上 之感測器61’，以及該與感測器61、61，相連接之超音波 儀。該運動裝置7則具有該樞接於該圍繞壁22上之滾輪 71 ° 該計數裝置8具有該至少一設置於滾輪71上之反光 20片81、該設置於圍繞壁22上並向滾輪71方向發射光線 之光源82、該設置於圍繞壁22上並能接收由反光片81 反射光源82所產生之光線的接收器83，以及該與接收器 83連接之計數器84。 惟以上所述者，僅為本發明之一較佳實施例而已，當 15 200417311 玖、發明說明（12) > 不能以此限定本發明實施之範圍，即大凡依本發明申請專 利範圍及發明說明書内容所作之簡單的等效變化與修飾， 皆應仍屬本發明專利涵蓋之範圍内。 _ 【圖式簡單說明】 5 第一圖是習知蔗糖偏好試驗設備之一部份剖面圖； 第二圖是本發明本發明動物興致程度評估方法及其設 備的一較佳實施例之一柱狀圖，說明僅提供清水時，黑色 小鼠一日之清水消耗量； · 第三圖是該較佳實施例之一柱狀圖，說明提供平行放 10 置之清水與蔗糖水時，黑色小鼠一日之清水與蔗糖水消耗 量； 第四圖是該較佳實施例之一柱狀圖，說明提供清水與 提高位置之蔗糖水時，黑色小鼠一日之清水與蔗糖水消耗 量； 15 第五圖是該較佳實施例之一流程圖； 第六圖是該較佳實施例之一立體圖； ® 第七圖是該較佳實施例之一立體分解圖； 第八圖是該較佳實施例之一柱狀圖，說明提供清水與 提高位置之蔗糖水時，在未受壓力下黑色小鼠一日之清水 20 與蔗糖水消耗量； 第九圖是該較佳實施例之一柱狀圖，說明提供清水與 提高位置之蔗糖水時，在承受壓力下黑色小鼠一日之清水 與蔗糖水消耗量； 第十圖是該較佳實施例之一柱狀圖，說明於實驗過程 16 200417311 玖、發明說明（13) 中，黑色小鼠一日跑滾輪之圈數；及 第十一圖是該較佳實施例之一側視示意圖，說明運動 裝置之配置關係。5 (Sucrose preference test) ^ * Willner P et al. Developed a method for measuring mice's interest in 1987 (or "Herdonia"), or "the degree of depression, and the" anti-anxiety agent response, "such as As shown in the first figure, the above-mentioned sucrose preference test was that a mouse 91 was previously harvested in an experimental mouth I 92 towel, and no water was given for 24 hours. Then, in the experimental cage 92, a clear water bottle% filled with clear water and a sugar water bottle 94 which is equivalent to the clear ruler bottle 93 and filled with 2-3% sucrose water, and will be replaced after half an hour The positions of the clear water bottle 93 and the sugar water bottle 94 are interchanged. After half an hour, remove the clear water bottle 93 and the sugar water bottle%, and measure the remaining amount of clear and sucrose water contained in 15 towels to know The amount of water and sucrose water consumed by the mouse 91 in the above-mentioned hour. According to the results of past research, the average mouse 91 has a preference for sweetened sugar water. Therefore, under normal conditions, the consumption of sucrose water will be higher than the consumption of clear water. According to Willner's research, if the 2 〇 Old milk 91's preference for sucrose water is regarded as an interest, and when stress or depression is lost, the mouse 91 is exposed to, or under pressure, or caused, depression In the state of, the mouse 91's interest in the preferred sucrose water will decrease, so that its intake ratio of the sucrose water will decrease. However, if an effective anti-worrying agent is injected into the mouse 91 200417311 发明, description of the invention (2), the interest in the sucrose water is restored due to the revival of the interest in the body. Therefore, the ratio of sucrose water intake It will also return to the general state described earlier. But according to the article "Chronic Mild Stress and Sucrose Consumption: Validity as a Model of Depression" by Naida F. Forbes et al. In Physiology & behavior, 5 Vol · 60 · Ν〇 · 6, ρρ · 1481-1484, 1996 , Pointed out that the same results can not be obtained by experimenting with the same sucrose preference test. In particular, after the sucrose water consumption was divided by the weight of the mouse 91 for correction, the amount of sucrose water consumed by the rat 91 at 10 weights per unit body did not change significantly. Therefore, the above-mentioned sucrose preference test considered that When the water bottle 93 and the sugar water bottle 94 containing sucrose water are placed in parallel, only the preference or demand of the mouse 91 for the sucrose water can be measured. For example, when the mouse 91 is hungry, it may ingest more sucrose water to supplement energy, and when it is thirsty, it may drink more clear water that quenches thirst, but it cannot support or prove that the consumption of sucrose water is the same as that of the mouse 91. Interest or depression are related, of course, the effect of applying pressure and the efficacy of injecting antidepressants cannot be verified. [Summary of the Invention] Therefore, the main object of the present invention is to provide a method and a device for evaluating the degree of animal interest. Another object of the present invention is to provide an animal behavior assessment method and equipment capable of distinguishing animal preferences and interests. Therefore, an animal interest assessment method according to the present invention includes the following steps: 6 200417311 发明, invention description (3) &) restricting an animal to an activity space; b) providing the animal to drink in the activity space A clear water 'and the animal that prefers to drink—the preferred liquid, and the preferred liquid obtaining position is higher than the clear water obtaining position; 5 e) measuring that the animal uses the clear water and preferred liquid amount in a predetermined period; and d) The amount of water and the preferred liquid that the animal drank was compared to evaluate the degree of interest of the animal during the predetermined period. And the animal interest degree evaluation equipment made according to the above method includes 10-box,-a clear water container provided on the box, and-a preferred liquid container provided on the box. The box has a base and a surrounding wall extending upward from the base, and the base and the surrounding wall can define a movable space. The clear water container has a clear water outlet communicating with the movable space and adjacent to the bottom, and the preferred liquid container has a preferred liquid outlet communicating with the movable space 15 and positioned higher than the clear water outlet. [Embodiment] The foregoing and other technical contents, features, and advantages of the present invention will be clearly understood in the following detailed description of the preferred embodiment with reference to the drawings. The meaning of 20 is that the test animal used in the preferred embodiment of the animal interest assessment method of the present invention is ten C57BL / 6 black mice, but it is not limited to this. For example, BALB / c. Mice and even other animals can use the present invention to evaluate the degree of interest. In this example, the average weight of these black mice is about 23 grams to 200417311 玖, description of the invention (4) grams, and is more active mainly at night, the second is the time that consumes more water, only for clear water At the same time, if the results obtained on the third day of the experiment prove that if the households need to make a sentence in the 12 hours in the evening, the water consumption is about 4.0 cc to 45 π ^ to supply clean water and sucrose water. Place them on the right side at the same time. As shown in the third picture, t is parallel to the same height of 0.5 cc to 1 cc of fresh water, and the intake of sucrose water of about 1.5 ^ to 6 _cc, the small black w Preference—T seeing that the difficulty of the special sugar water increases' ^ 7 and the right will get the sugar cane and return, for example, placing the sugar sugar water in a position where the black mice must pay and return, as in the fourth station- When w can get the recommended sugar water, = the four pictures do not, wait for the black mice-the day will be 1.5CC of water and 3 · 5 He to recommend sugar water is not the black mice γ ^ γ 'can be seen due to the above life and Survival will not increase two more: go: use, and will not hinder its life ^ ^ ^ Pain. Therefore, when these black mice are willing to spend extra labor or trouble to 'take ¥, they can show their interest. Therefore, the present invention is based on the preference food that can obtain axe sugar water within the range of animal abilities and the living food for 4 people who reach the moon water as clear as the night of Qingxi, and when the animal can choose freely, by increasing the Difficulty in the process of obtaining food preferences to achieve the ability to identify interests or preferences. As shown in Figures 5 to 7, the preferred embodiment of the method for assessing the degree of interest in animals of the present invention includes the following steps: In this implementation, the animal 1 to be evaluated is a step-like black mouse. i is confined to an activity space 20. On tM U.l. JU JO-, 〇 ·· · · 1 20 200417311 玖, invention description (5) Activity space 20 is defined by _ γβ _ 疋 疋 疋 疋 甶 体 2. The box 2 has a base 21 & a surrounding wall extending upward from 4 bases 21 The base 1 ... Xuanwei, Fanbi 22 defines the activity space that restricts the movement of animals i 20 〇 Step 102, provide animals! The drinking π water can be obtained in the activity space 20. Here, a clear water container 3 containing clear water for animals to live on is provided on the box 2. The clear water container 3 has a hollow outer sleeve. 31 and a piston η provided in the outer sleeve 3 i, the piston 32 and the outer sleeve 31 are tightly merged to form a clear water container containing water. 310 The outer sleeve 31 is further formed with a movable space. 20 is connected to and adjacent to the clear water outlet 30 of the base 21, and the animal i consumes clear water through the clear water outlet 30 to drink. Step 104, provide animals! Obtain a position within 20 of the activity space. Obtain a position between 4 π water, and it is one of the preferred liquids for animals. In this embodiment, the preferred liquid is sucrose water, which is of course not limited to this. The preferred liquid may also be a cola or an alcoholic beverage. Similarly, the preferred liquid is contained in a preferred liquid container 4, and the preferred liquid container 4 is also disposed on the case 2 and has a hollow outer sleeve 41 and a penetrating through the outer sleeve 41. The inner piston 42 and the 20 ° helical piston 42 are tightly merged with the outer sleeve 41 to form a preferred liquid container 4 i 〇. The outer sleeve 41 is further formed with the preferred liquid outlet 40 which communicates with the movable space 20 and is far from the base 21, so that the position of the preferred liquid outlet 40 is higher than the position of the clear water outlet 30. The box 2 further has a pipe ladder 5 provided on the surrounding wall 22, and the pipe ladder 5 has a hollow and 200417311 玖, description of the invention (6) if the belly of the bellows 52 continuously undulates, a A channel 520 'in the tube body 52 and two end portions 51, 51' located at two opposite ends of the tube body 52. The end portions 51, 51, one of which is adjacent to the preferred liquid outlet 40 and connected to the preferred liquid container 4, and the end portions 51 5 and the other end portion 51 are adjacent to the base 21. The animal 1 can access the preferred liquid container 4 through the passage 520 of the pipe ladder 5 and obtain the preferred liquid from the preferred liquid outlet 40 for drinking. Step 106: Measure the amount of the clear water and the preferred liquid that the animal 1 drinks during a predetermined period. Since the piston 32 of the clear water container 3 is provided with a sensor 61 for measuring the liquid level of the clear water in the clear water chamber 310, the piston 42 of the preferred liquid container 4 is also provided with a sensor The sensor 61 for measuring the liquid level of the preferred liquid in the preferred liquid container 41. In this embodiment, each of the sensors 61 and 61 ′ is an ultrasonic transceiver, and The sensors 61 and 01 are further connected to an ultrasonic instrument (not shown), and can accurately and automatically record the consumption of the clear water in the preferred liquid. The predetermined period is one day in this embodiment. Of course, the predetermined period can be designed with different cycles according to the needs of the experiment, and is not limited to one day. According to the experimental results, in the general state, as shown in the fourth figure, animal 1 will consume about 1.0 CC to 1.5 CC of fresh water and 3.5 CC to 4_0 cc of preferred liquid during the 20 predetermined period. Relative to the consumption of about 0.5 cc to 1 cc / monthly water and the preferred liquid sucrose water 5.5 cc to 0.0 cc, which are placed in parallel at the same height as shown in the third figure, it shows that the preferred liquid container 4 is indeed elevated. It can increase the difficulty for animal 1 to obtain the preferred liquid. 10 发明 、 Explanation of the invention (7) Another first-grade experiment about whether pressure can reduce animal 1's preference for drinking liquids, as shown in the figure above. Animal 1 prefers liquid consumption higher than water when not under pressure. Consumption, and the average gap is about 2 cc; while under pressure ft 'as shown in the ninth figure, animal 1's preferred liquid consumption is not the same as water consumption / shaw consumption, which is numbered 2, 3 No. 5, No. 5, No. 6 and No. 8 and No. 10 black mice still maintain preference for liquid consumption and set south to clear water consumption, but No. 丨, No. 4, No. 7 and No. 9 preference for black mice Liquid consumption was significantly lower than water consumption. 10 Step 108: Measure the activity of Animal 1 during the predetermined period. As shown in the sixth and tenth figures, a movement device 7 connected to the movable space 20 and adjacent to the base 21 is installed on the side of the box 2. The exercise device 7 has a roller 71 pivoted on the surrounding wall 22 for the black mouse. A counting device 8 is further installed on the box 2 and the roller 71, and the 15 counting device 8 has at least one reflective sheet 81 disposed on the roller 71, and one is disposed on the surrounding wall 22 and faces the roller 71. A light source 82 that emits light, a receiver 83 disposed on the surrounding wall 22 and capable of receiving light generated by the light reflecting plate 81 reflecting the light source 82, and a counter 84 connected to the receiver 83. Because the roller 71 does not reflect the light emitted by the light source 82, the roller 71 will rotate to a specific position within a rolling range of the circle, so that the receiver 83 just receives a reflective sheet The light signal reflected by 81, at this time, the counter 84 will record one rotation of the roller 71. Since measuring the number of running laps of animal 1 within the predetermined period, 11 200417311 can be calculated. (8) Activity amount of animal 1. Therefore, the counter 84 directly calculates the number of revolutions of the roller 71 during the predetermined time, so that the health status of the animal can be estimated. As shown in the eleventh figure, when the above experimental control was carried out due to the pressure that reduced the interest of Animal 1, the black mice tested ran an average of about 5 15,000 to 20,000 rollers 71 per day. Step 110: Compare the amount of water and preferred liquid that animal i drinks to evaluate the degree of interest of animal i during the predetermined period. According to the results obtained according to the above experimental steps, it is found that in the general state of animal i, as shown in the eighth figure, the animal's preferred liquid consumption is about 2cc higher than the water consumption; and H) when under pressure, As shown in the ninth figure, the preferred liquid consumption of animal i is not the same as the consumption of clean water. The preference liquid consumption of black mice numbered i, number 4, number 7, and number 9 is significantly lower than that of water. The black mice numbered 2, 3, 5, 5, 8, and 10 seemed to be unaffected by the applied pressure, which clearly showed that each black mouse was subjected to the same force. The response was different. vStep 112 'corrects the degree of interest of animal 1 during the predetermined period according to the amount of activity of the animal in the predetermined period. Since the difficulty of obtaining the preferred liquid is high, additional labor is required to obtain it. Therefore, the health status of Animal 1 must be monitored to ensure that it still meets the original settings, so that the conditions for obtaining the preferred liquid are still maintained in the animal! Within their capabilities. According to the animal obtained in step 108] Animal 1 runs the number of turns of the roller 71 in the predetermined period, and then the estimated degree of interest estimated in step U0 can be corrected, and the ability of the preference ㈣ exit 40 to still be located in the animal i can be confirmed Within the range, the experimental results obtained to confirm tolerance are valid. Taiji This practical example is to exclude the experimental data of the running roller 71 ® number less than 12 200417311 发明, invention description (9) 3,000 laps, as shown in the tenth figure, whether the pressure is applied or not, in the experiment During the process, none of the black mice ran the wheel with 71 turns less than 3,000. In step 114, the evaluation of the degree of interest of the animal is completed. 5 In the above method for assessing animal interest, step 110 evaluates the interest of animal i by using animal i 动物 the water and preferred liquid consumption, as shown in the ninth figure, and the result shows that under pressure, animal i The preference for liquid consumption is not the same in the consumption of water. It is clearly shown that after being subjected to the same pressure, the interest of each black mouse changes to varying degrees 10 ′ and not all black mice will reduce interest, showing the present invention. Can distinguish individual differences that cannot be distinguished by traditional methods in the past. ^ In addition, the clean water and preferred liquids provided in steps 102 and 104, and month b are replaced by different vitamins and preferred foods, but the vitamins and preferred foods must be able to clearly distinguish evil. As for the feasibility of the implementation, compared with the clean water container 3 containing clear water and the liquid preference liquid container 4, the living food container suitable for storing living foods and the suitable storage Preferred food containers are replaced by preferred food. In the above step 106, the sensors 61 and 01 are used in order to achieve continuous and automatic monitoring, but it is not limited to this. Since step 106 is for measuring the clear water and the preferred liquid. Consumption, so right to change to measure by weight, or even read the capacity scale by manpower, etc., can also achieve the same purpose. In addition, 'because the preferred liquid outlet 4 of the preferred liquid container 4 0413 200417311 发明, description of the invention (10) the installation of the pipe ladder 5 away from the outlet 30 is located at ~ 51, facing the clean water body The difficulty is to make animal 1 get the preference solution and increase it to separate the interest and preference of animal 1 and improve the ability of Zhongbei interest or «. Therefore, the artist who is familiar with this = Γ can easily infer that as long as it can increase the animal 1's access to or obtain the preference:: The process is relatively difficult compared to the living food, so that the animal i: can obtain the preferred food with extra labor. For example, methods such as ramps and two-hybrid switches can also achieve the same effect, and are the invention; < It must be explained here that if animal 1 is caused to suffer during the process of obtaining the preferred liquid, for example, an electric shock device (not shown) is installed on the pipe ladder 5, and the animal is still willing to endure the pain When it is taken, it means that Animal 1 has money and has dependence on the lack of liquid, and the pain of not drinking will be greater than the pain caused by electric shock. When the above-mentioned addiction phenomenon occurs in Animal 1, it indicates that its experimental results are non-interesting behaviors, and therefore it can also be used as a verification of the animal interest evaluation method of the present invention. According to the above evaluation method, it can be known that the apparatus for evaluating the degree of animal interest of the present invention includes the case 2, the fresh water container 3, the preferred liquid container 4, 20, the measuring device 6, the exercise device 7, and the counting device 8. The box 2 has the base 21, a surrounding wall 22 extending upward from the base 21, and a pipe ladder provided on the surrounding wall 22. The box 2 is formed with an activity space 20 defined by the base 21 and the surrounding wall 22 and for the animal 1 to move. The clear water valley state 3 is arranged on the box body 2 and has the 14 200417311 玖, the second outer sleeve of the invention description (11) is the same as the 3, the piston 3 penetrating inside the outer sleeve 3 1, and the The fresh water volume 31 defined by the piston 32 and the outer sleeve 31, and the clear water outlet% formed on the outer sleeve 31 and communicating with the movable space 20%. The preferred liquid container 4 is provided on the case 2 And has the hollow outer sleeve 41, the piston 42 disposed in the outer sleeve 41, the preferred liquid container 41 defined by the piston 42 and the outer sleeve 41, and the formed in the outer sleeve The preferred liquid outlet 40 on 41 is also in communication with the activity space 20. The position of the preferred liquid outlet 40 is higher than the position of the clean water outlet 30. The pipe ladder 5 has the hollow pipe body 52, the passage 520 formed in the pipe body 52, the end portion 51 located on the pipe body 52 and adjacent to the preferred liquid outlet 40, and the oppositely located on the pipe body 52 The end portion 51 is located at and adjacent to the end portion 51 of the base 21. The measuring device 6 has the sensor 61 installed on the piston 32 15 of the clean water container 3, the sensor 61 ′ installed on the piston 42 of the preferred liquid container 4, and the sensor Devices 61, 61, connected ultrasonic instruments. The moving device 7 has the roller 71 pivoted on the surrounding wall 22. The counting device 8 has the at least one reflective 20 piece 81 provided on the roller 71. The counting device 8 is provided on the surrounding wall 22 and faces the roller 71. A light source 82 that emits light, a receiver 83 disposed on the surrounding wall 22 and capable of receiving light generated by the light reflector 82 reflecting the light source 82, and a counter 84 connected to the receiver 83. However, the above is only one of the preferred embodiments of the present invention. When 15 200417311 玖, the description of the invention (12) > can not be used to limit the scope of the invention, that is, the scope of the patent application and invention according to the invention The simple equivalent changes and modifications made in the description should still fall within the scope of the patent of the present invention. _ [Schematic description] 5 The first figure is a partial cross-sectional view of a conventional sucrose preference test device; the second figure is a column of a preferred embodiment of the method and apparatus for evaluating animal interest according to the present invention A diagram showing the consumption of water by black mice in one day when only water is provided; The third diagram is a histogram of one of the preferred embodiments. It shows that when providing 10 sets of water and sucrose water in parallel, the black color is small. Consumption of clean water and sucrose water in a rat in a day; Figure 4 is a histogram of one of the preferred embodiments, illustrating the consumption of clean water and sucrose water in a black mouse in a day when fresh water and sucrose water in an elevated position are provided; 15 The fifth diagram is a flowchart of the preferred embodiment; the sixth diagram is a perspective view of the preferred embodiment; ® the seventh diagram is an exploded perspective view of the preferred embodiment; the eighth diagram is the comparative view A histogram of one of the preferred embodiments illustrates the consumption of clean water 20 and sucrose water in black mice in a day without pressure when providing clean water and raised sucrose water; Figure 9 is one of the preferred embodiments Histogram illustrating water supply and elevation When sucrose water is placed, the consumption of fresh water and sucrose water by black mice under pressure for one day; Figure 10 is a histogram of one of the preferred embodiments, illustrated in the experimental process 16 200417311 发明, Description of the invention (13 ), The number of turns of a black mouse running a day; and FIG. 11 is a schematic side view of one of the preferred embodiments, illustrating the configuration relationship of the exercise device.
17 200417311 玖、發明說明（14) 【圖式之主要元件代表符號簡單說明】 1 動物 51 端部 2 箱體 52 管體 20 活動空間 520通道 21 底座 6 感測裝置 22 圍繞壁 61 感測器 3 清水容器 7 運動裝置 30 清水出口 71 滾輪 31 外套筒 8 計數裝置 32 活塞 81 反光片 310清水容室 82 光源 4 偏好液體容器 83 接收器 40 偏好液體出口 84 計數器 41 外套筒 91 老鼠 42 活塞 92 實驗籠 410偏好液體容室 93 清水瓶 5 管梯 94 糖水瓶 100.102.104·106.108_110·112·114.步驟17 200417311 发明. Description of the invention (14) [Simplified description of the main elements of the diagram] 1 animal 51 end 2 box 52 tube 20 movable space 520 channel 21 base 6 sensing device 22 surrounding wall 61 sensor 3 Clear water container 7 Movement device 30 Fresh water outlet 71 Roller 31 Outer sleeve 8 Counting device 32 Piston 81 Reflector 310 Clear water tank 82 Light source 4 Preferred liquid container 83 Receiver 40 Preferred liquid outlet 84 Counter 41 Outer sleeve 91 Mouse 42 Piston 92 The experimental cage 410 prefers a liquid container 93 a water bottle 5 a tube ladder 94 a sugar water bottle 100.102.104 · 106.108_110 · 112 · 114.
1818