TR2021013445A2 - CERAMIC NANOFIBER BASED ELECTROCHEMICAL GLUCOSE SENSOR TO BE USED AS A CONSUMABLE IN BLOOD SUGAR MEASUREMENT - Google Patents
CERAMIC NANOFIBER BASED ELECTROCHEMICAL GLUCOSE SENSOR TO BE USED AS A CONSUMABLE IN BLOOD SUGAR MEASUREMENTInfo
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- TR2021013445A2 TR2021013445A2 TR2021/013445A TR2021013445A TR2021013445A2 TR 2021013445 A2 TR2021013445 A2 TR 2021013445A2 TR 2021/013445 A TR2021/013445 A TR 2021/013445A TR 2021013445 A TR2021013445 A TR 2021013445A TR 2021013445 A2 TR2021013445 A2 TR 2021013445A2
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- 239000008280 blood Substances 0.000 title claims abstract description 8
- 210000004369 blood Anatomy 0.000 title claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims description 18
- 239000008103 glucose Substances 0.000 title claims description 18
- 239000002121 nanofiber Substances 0.000 title claims description 12
- 239000000919 ceramic Substances 0.000 title claims description 8
- 238000005259 measurement Methods 0.000 title claims description 5
- 238000000034 method Methods 0.000 claims description 19
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 14
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 14
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 13
- 238000001523 electrospinning Methods 0.000 claims description 12
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 229920000642 polymer Polymers 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 239000002086 nanomaterial Substances 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 239000006185 dispersion Substances 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 230000005684 electric field Effects 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000007970 homogeneous dispersion Substances 0.000 claims description 2
- 229910052763 palladium Inorganic materials 0.000 claims description 2
- 229910052709 silver Inorganic materials 0.000 claims description 2
- 239000004332 silver Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000004753 textile Substances 0.000 claims description 2
- 230000000977 initiatory effect Effects 0.000 claims 1
- 238000003760 magnetic stirring Methods 0.000 claims 1
- 238000007650 screen-printing Methods 0.000 claims 1
- 239000002105 nanoparticle Substances 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 239000002131 composite material Substances 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 229910021389 graphene Inorganic materials 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- -1 Ag+ ion Chemical class 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 239000000956 alloy Substances 0.000 description 2
- 229910045601 alloy Inorganic materials 0.000 description 2
- 238000001354 calcination Methods 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 239000003575 carbonaceous material Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
- 150000003624 transition metals Chemical class 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Ecology (AREA)
- Electrochemistry (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
Buluş, kan şekerinin rutin kontrolü amacıyla kullanılan elektronik ölçüm cihazlarının kan örneğine temas eden kısmındaki yenilik ile ilgilidir.The invention relates to the innovation in the part of the electronic measuring devices used for routine control of blood sugar, in contact with the blood sample.
Description
TARIFNAME KAN SEKERI ÖLÇÜMÜNDE SARF MALZEMESI OLARAK KULLANILMAK ÜZERE SERAMIK NANOLIF ESASLI ELEKTROKIMYASAL GLIKOZ SENSÖRÜ TEKNIKALAN Bulus, kan sekerinin rutin kontrolü amaciyla kullanilan elektronik ölçüm cihazlarinin kan örnegine temas eden kismindaki yenilik ile ilgilidir. ÖNCEKI TEKNIK Glikoz sensörünün yillardir kullanildigi uygulamalarda genellikle benzer yöntemler izlenerek farkli tasarimlarda ürünler piyasaya sürülmüstür. Bu ürünlerle ilgili patentler mevcut olup buna ilave olarak çok fazla sayida yayin da yapilmistir. DESCRIPTION TO BE USED AS A CONSUMABLE IN BLOOD SUGAR MEASUREMENT CERAMIC NANOLIF BASED ELECTROCHEMICAL GLUCOSE SENSOR TEKNIKALAN The invention is an electronic measurement used for routine control of blood sugar. It is related to the innovation in the part of the devices that contact the blood sample. PRIOR ART In applications where the glucose sensor has been used for years, it is generally similar. By following the methods, products in different designs have been put on the market. with these products There are related patents and in addition to this, a large number of publications have been made.
Mevcut teknikte, glikoz sensörü üretimi, yüksek seçicilik ve algilama performansi elde etmek için enzim kullanimina dayanmaktadir. Diger yandan, biyolojik elementin glikoz sensörlerinde kullanilmasi, karmasik enzim immobilizasyon süreci ve yüksek maliyet, enzim tabakasinin düsük kararliligi ve es zamanli kisa raf ömrü gibi bazi dezavantajlari ortaya koymaktadir. Bu zorluklarin tümü, enzimatik glikoz sensörlerinin daha da gelismesini engellemektedir. In the current art, glucose sensor fabrication, high selectivity and detection It relies on the use of enzymes to achieve performance. On the other hand, use of biological element in glucose sensors, complex enzyme immobilization process and high cost, low stability of the enzyme layer and simultaneous short shelf life. It presents some disadvantages such as longevity. All of these challenges are enzymatic inhibits the further development of glucose sensors.
BULUSUN KISA AÇIKLAMASI Yukarida belirtilen eksiklikleri gidermek için, glikoz redoks reaksiyonlari için enzim yerine katalitik olarak aktif nanomalzemelerin kullanilmasina bagli olan enzimatik olmayan kari glikozunun tespiti, son birkaç yilda odak noktasi olmustur. BRIEF DESCRIPTION OF THE INVENTION To compensate for the above-mentioned shortcomings, for glucose redox reactions due to the use of catalytically active nanomaterials instead of enzymes. The detection of non-enzymatic snow glucose has been the focus of attention in the last few years.
Nanomalzemelerin katalitik özelliklerinden en iyi sekilde yararlanmak için arastirmacilar farkli yöntemler denemektedirler. To make the most of the catalytic properties of nanomaterials Researchers try different methods.
Basvurumuz kapsaminda ortaya konan bulusumuzda, elektroegirme teknigi ile essiz kafes yapili nanomalzeme elde edilmistir. Böylece daha kararli ve hassasiyeti yüksek glikoz sensörleri üretilebilmektedir. In our invention, which was presented within the scope of our application, we used electrospinning technique. A unique lattice-structured nanomaterial was obtained. Thus, it is more stable and sensitive. high glucose sensors can be produced.
Enzimatik esasli ölçüm çubuklarini sürekli kullanan diyabet hastalarinin çok iyi bildigi gibi, bu ürünlerle ölçülen degerlerde belli bir süre sonunda önemli hatalar meydana gelmektedir ve çözüm olarak hemen yenisinin temin edilmesi önerilmektedir. Bulusumuzla birlikte bu tür sorunlarin yasanma sikligi azalmaktadir. Diabetic patients who constantly use enzymatic-based dipsticks have very good results. As you know, there are significant errors in the measured values with these products after a certain period of time. occurs, and a new one is immediately available as a solution. recommended. With our invention, the frequency of such problems is decreasing.
SEKIL LISTESI Sekil 1. Üretilen Kafes Seklinde Nanoliflerin SEM Görüntüsü BULUSUN DETAYLI AÇIKLAMASI Bulusumuz paladyum (Pd), gümüs (Ag) ve grafenoksit (GO) nanomalzemelerinin belirlenmis oranlarda ve yeni bir yöntemle kafes seklinde elde edilmesi esasina dayanmaktadir. Pd gibi çok sayida özel yapim asil metal ve metal oksit nanoyapilarin glikoz ve diger analitlere karsi mükemmel elektrokatalitik algilama özellikleri gösterdigi bilinmektedir. Pd nanopartiküller (NP'Ier) iyi kimyasal kararlilik, mükemmel elektriksel iletkenlik ve dikkate deger elektro-katalitik performans dahil olmak üzere üstün kimyasal ve elektrokimyasal özellikleri nedeniyle büyük ilgi görmektedir. Bununla birlikte, Pd NP'lerin sentezi sirasinda reaktiviteyi, kararliligi, geri dönüstürülebilirligi ve özellikle algilama performansini etkileyebilen aglomerasyon meydana gelebilir. Bu eksikliklerin üstesinden gelmek için, bir miktar geçis metali ile Pd metaline dayali nano alasimlarin imalati, güçlü etkilesimlerinin bir sonucu olarak artan sinerjistik etki nedeniyle etkili bir stratejidir. Ek olarak, alasim olusumu, yapi içindeki elektronik bulutu siklikla etkileyen kusurlarin olusmasina ve dolayisiyla elektron transferi için artan elektro-aktif bölgelerin olusmasina neden olur. LIST OF FIGURES Figure 1. SEM Image of Lattice-Shaped Nanofibers Produced DETAILED DESCRIPTION OF THE INVENTION Our invention consists of palladium (Pd), silver (Ag) and graphenoxide (GO). nanomaterials are obtained in the form of a lattice in determined proportions and with a new method. is based on. Numerous custom-made noble metals and metals such as Pd Excellent electrocatalytic detection of oxide nanostructures against glucose and other analytes. known to exhibit properties. Pd nanoparticles (NPs) have good chemical stability, including excellent electrical conductivity and remarkable electro-catalytic performance great interest due to its superior chemical and electrochemical properties, including sees. However, during the synthesis of Pd NPs, its reactivity, stability, which can affect recyclability and especially detection performance. agglomeration may occur. To overcome these shortcomings, some The fabrication of transition metal and Pd metal-based nano-alloys is one of their strong interactions. It is an effective strategy because of the increased synergistic effect as a result. Additionally, alloy Its formation causes defects that often affect the electronic cloud within the structure and therefore, it causes the formation of increased electro-active sites for electron transfer.
Ag+ iyonu güçlü antibakteriyel özellik ve çesitli bakteriyel propagüller, bakteri sporlari, virüsler, mantarlar ve diger mikroorganizmalara karsi mükemmel ve genis spektrumlu antibakteriyel aktivite gösterir. Ayrica az miktarda Ag+ iyonu ile katkilanmis kompozit materyallerin insan vücudu için toksik olmadigi bildirilmistir. Ag+ ion has strong antibacterial properties and various bacterial propagules, bacteria Excellent and broad spectrum against spores, viruses, fungi and other microorganisms. Shows broad spectrum antibacterial activity. Also with a small amount of Ag+ ions It has been reported that the added composite materials are not toxic to the human body.
Diger gümüs sekli olarak, Ag NP'Ier sadece mükemmel antibakteriyel ve elektriksel iletkenlik sergilemekle kalmaz, ayni zamanda genis spesifik yüzey alanina ve güçlü yüzey aktivitesine sahiptir. Ag NP'ler, nanofiberlerin antibakteriyel özelliklerini ve iletkenligini gelistirmek için de seçilmistir. In other silver form, Ag NPs are not only excellent antibacterial and electrical conductivity, but also has a large specific surface area and strong It has surface activity. Ag NPs enhance the antibacterial properties of nanofibers and It was also chosen to improve its conductivity.
Karbon-esasli malzemelerin sahip oldugu essiz özellikler, elektrokimyasal yöntemler kullanilarak biyolojik önemi yüksek olan çok sayida molekülün yüksek hassasiyette, hizli ve güvenilir bir sekilde tayininin yapilmasina olanak saglamaktadir. The unique properties of carbon-based materials, electrochemical high number of molecules of high biological importance by using methods It allows determination of sensitivity, fast and reliable way.
Grafen, karbon atomlarinin sp2 hibritlesmesi sonucu iki boyutta hekzagonal dizilimi sonucu olusan atomik seviyede ince bir tabakadir. Grafenin bu atomik dizilimi essiz derecede yüksek bir elektriksel iletkenlik, mekanik mukavemet ve çok yüksek fiziksel yüzey alani saglamaktadir. GO aslinda yüzeyinde bazi fonksiyonel gruplar bulunduran grafendir. GO sahip oldugu üstün mekanik özellikleri nedeniyle seramik kompozitler için güçlü bir destek elemanidir. Benzersiz elektriksel ve termal özellikleri genis uygulama alanlarina sahip çok fonksiyonlu seramik üretiminde ilgi çekici bir katki maddesidir. Grafen destekli seramikler grafenin katkisindan dolayi büyük bir potansiyele sahip olmaktadirlar. Graphene is a two-dimensional hexagonal arrangement of carbon atoms as a result of sp2 hybridization. The result is a thin layer at the atomic level. This atomic arrangement of graphene is unique. extremely high electrical conductivity, mechanical strength and very high physical provides surface area. GO actually has some functional groups on its surface. containing graphene. GO is ceramic due to its superior mechanical properties. It is a strong support element for composites. Unique electrical and thermal properties It is an interesting product in the production of multifunctional ceramics with wide application areas. is an additive. Graphene-supported ceramics have a great impact due to the contribution of graphene. they have potential.
Elektrospinleme yöntemi kompozit malzemeyi birkaç yüz nanometre çapinda bir lif içine tutturarak ve bu liflerin dönerek ulastigi toplaç üzerinde üç boyutlu kafes yapiyi olusturarak ideal bir sensör malzeme yapisi saglamaktadir. Bu amaçla bulusumuzda elektrospinleme yönteminden yararlanilmistir. Bu yöntem ayrica iyon halindeki metallerin (Ag+ ve Pd2+) yüksüz nanoparçaciklara dönüsmesini de tek islemle yapma imkâni saglamaktadir. Elektrospinleme yöntemiyle üretilen nano liflerin özelliklerini, derisim, siringa ucu ve toplayici arasi mesafe, akis hizi gibi parametreler etkilemektedir. The electrospinning method cuts the composite material into a few hundred nanometers in diameter. three-dimensional lattice on the top that these fibers reach by attaching them to a fiber and rotating It provides an ideal sensor material structure by forming the structure. To this end Electrospinning method was used in our invention. This method also It is the only way to transform metals (Ag+ and Pd2+) into uncharged nanoparticles. provides the opportunity to do business. Nano produced by electrospinning method properties of the fibers, such as concentration, distance between syringe tip and collector, flow rate. parameters affect.
Bulusumuzun üretilme adimlari su sekildedir: - Öncelikle destek polimeri olan polivinil alkol (PVA) polimerinin %10'luk sudaki çözeltisi homojen bir sekilde hazirlanmistir. PVA biyouyumlulugu yüksek, suda çözünebilen ve kalsinasyon sonucu ortamdan kolayca uzaklastiralabilen birlestirici bir malzeme olup muadillerine göre temini ve fiyati da rekabetçi oldugu için tercih edilmistir. PVA'nin suda hazirlanan çözeltisinin optimum derisimi, homojenlik, viskozite ve elektrospinleme asamasinda çözücünün istedigimiz hizla buharlasmasi gibi kriterlere uydugu için kütlece %10 olarak belirlenmistir. - 10 mL 2 mg/mL GO (grafenoksit) dispersiyon çözeltisi içine kati toz halinde karistirilmistir. The production steps of our invention are as follows: - First of all, 10% of the polyvinyl alcohol (PVA) polymer, which is the support polymer, its solution in water was prepared homogeneously. PVA biocompatibility high, water-soluble and easily removed from the environment as a result of calcination. It is a bonding material that can be removed and can be supplied and used according to its equivalents. It is preferred because its price is also competitive. PVA prepared in water optimum concentration of solution, homogeneity, viscosity and electrospinning At the stage of evaporation of the solvent at the speed we want, criteria such as It has been determined as 10% by mass because it fits. - 10 mL of 2 mg/mL GO (graphenoxide) as solid powder into dispersion solution mixed.
- Daha sonra bu karisim 60°C'ye getirilen %10'luk PVA içine eklenip manyetik karistiricida iyice karistirilarak çözündürülmüstür. Böylece kompozit malzememiz için gereken tüm bilesenleri bulunduran elektrospinleme çözeltisi hazirlanmistir. - Then this mixture is added to 10% PVA brought to 60°C and It was dissolved in a magnetic stirrer by mixing it well. Like this containing all the components required for our composite material The electrospinning solution was prepared.
- GO miktari %10”Iuk PVA içinde %1 oranda ayarlanmistir. GO kimyasal olarak sahip oldugu özelliklerinden dolayi tabakalari arasinda kuvvetli bir çekim bulunmaktadir ve bulundugu çözeltinin fiziksel özelliklerini önemli ölçüde etkilemektedir. Hem gereken katkiyi verebilecegi hem de elektrospinleme islemine engel olmayacagi bir derisimin seçilmesi gerekmektedir. - The amount of GO is adjusted at 1% in 10%”Luk PVA. GO chemical due to its properties as a strong There is attraction and the physical properties of the solution in which it is found are important. extent affect. It can both give the necessary contribution and Choosing a skin that will not interfere with the electrospinning process required.
Bu karisimda PVA, destek polimeri özelligi göstermekte olup, nanoparçaciklarin kafes yapisinda düzenlenmelerine yardimci olmaktadir. PVA ana çözelti ortaminda bulunan kompozit malzemeler elektrospinleme islemi sonucu nanometre seviyesinde çapa sahip iplikçikler haline gelmektedir. Bu yapidaki iplikçiklerin üst üste birikerek çok katli ag biçiminde toplanmasi üç boyutlu kafes yapinin olusmasini saglamaktadir. Bu sekilde elde edilen nanolif yapili tekstil malzemeden, kalsinasyon islemi sonucu ortamda bulunan organik yapidaki bilesen, yani PVA uzaklastirilip ve seramik malzemeden üç boyutlu kafes yapiya sahip malzeme elde edilir. Çözelti hazirlama asamasinda, hazirladigimiz çözeltiden elektrospinleme yöntemiyle nanolif elde edilmesi için yapilan denemeler sonucunda en uygun boyut ve özellikte nanolif elde ettigimiz yöntemin ayrintilari asagida verilmistir; - Ilk olarak siringaya 5 mL polimer çözeltisi çekilerek, siringa pompasina yerlestirilir. In this mixture, PVA shows support polymer properties, It helps the nanoparticles to be arranged in a lattice structure. PVA main Composite materials in solution environment are the result of electrospinning process. It becomes filaments with a diameter of nanometers. in this build three-dimensional lattice it creates the structure. The nanofiber fabric obtained in this way The organic component found in the environment as a result of the calcination process from the material, that is, the PVA is removed and the ceramic material has a three-dimensional lattice structure. material is obtained. In the solution preparation stage, electrospinning from the prepared solution As a result of the trials made to obtain nanofibers by the method of and the details of the method in which we obtained nanofibers are given below; - First, 5 mL of polymer solution is drawn into the syringe, and it is pumped into the syringe pump. is placed.
- Yüksek gerilim güç kaynagi araciligiyla siringa ucuna güç kaynagindan 11,5 kV yüksek gerilim uygulanir, - Çözelti akis hizi siringa pompasindan saatte 0,3 mL olarak ayarlanir ve elektrospinleme süreci baslatilir. - From the power supply to the syringe tip via the high voltage power supply 11.5 kV high voltage is applied, - The solution flow rate is set at 0.3 mL per hour from the syringe pump and The electrospinning process is started.
- Siringadan çikan polimer, uygulanan yüksek gerilim sayesinde olusan elektriksel alan etkisiyle, nanolif olusturulur. - The polymer coming out of the syringe is formed by the high voltage applied. With the effect of electric field, nanofiber is formed.
Tekstil halinde elde edilen ve PVA/GO/Ag/Pd karisimindan ibaret nanoliflerin 400°C'de kül firininda isil isleme tabi tutulmasi ile toz halinde ve kafes yapiya sahip seramik sensör malzemesi elde edilir. Bu islemle ayni zamanda destek polimeri olarak kullanilan PVA da ortamdan uzaklastirilmis olur. Elde edilen sensör malzemesi daha sonra dimetil formamit çözücüsü içinde mümkün oldugu kadar homojen bir dispersiyon (dagilmis) haline getirilir ve damlalik yardimiyla karbon ekran baski elektrot yüzeyine damlatilir. Dimetil formamit sahip oldugu fiziksel ve kimyasal özellikleri dolayasiyla iyi bir dispersiyon çözücüsüdür. Homojen bir dispersiyon hazirlanmasina uygun olmasinin yaninda, kurutma asamasinda da ortamdan kolayca uzaklastigi için tercih edilmistir. Kuruduktan sonra glikoz sensörü hazir hale gelmis olur. Nanofibers obtained in the form of textiles and consisting of a mixture of PVA/GO/Ag/Pd It is in powder form and has a cage structure after heat treatment in an ash oven at 400°C. ceramic sensor material is obtained. At the same time with this process, the support polymer PVA, which is used as a solvent, is also removed from the environment. The resulting sensor material is then dissolved in dimethyl formamide solvent as much as possible. it is made into a homogeneous dispersion (dispersed) and carbon screen print is dripped onto the electrode surface. Dimethyl formamide has physical and It is a good dispersion solvent due to its chemical properties. a homogeneous In addition to being suitable for dispersion preparation, it is also suitable for drying. It is preferred because it is easily removed from the environment. Glucose sensor after drying it will be ready.
Elde ettigimiz sensör, normal kosullarda ve geleneksel bir elektrot ile belirlenmesi çok zor olan glikozun, daha kolay tespit edilmesini saglamaktadir. The sensor we obtained is under normal conditions and with a conventional electrode. It enables glucose, which is very difficult to determine, to be detected more easily.
Piyasada bulunan ve yaygin bir sekilde kullanilan glikoz sensörlerinde katalizör olarak islev gören enzim yerine, bizim ürettigimiz sensör malzemesinde bulunan Pd, Ag ve GO malzemeleri benzer etki göstererek glikozun yükseltgenme tepkimesini hem kolaylastirmakta hem de hizlandirmaktadir. Böylece glikozun varligini ve derisiminin tespit edilebilecegi bir sinyal elde edilmektedir. Catalyst in commercially available and widely used glucose sensors Pd, which is in the sensor material we produce, instead of the enzyme that functions as a Ag and GO materials have similar effects, causing the oxidation reaction of glucose. It both simplifies and speeds up. Thus, the presence of glucose and A signal is obtained from which the concentration can be detected.
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| TR2021/013445A TR2021013445A2 (en) | 2021-08-25 | 2021-08-25 | CERAMIC NANOFIBER BASED ELECTROCHEMICAL GLUCOSE SENSOR TO BE USED AS A CONSUMABLE IN BLOOD SUGAR MEASUREMENT |
| PCT/TR2022/050871 WO2023027667A1 (en) | 2021-08-25 | 2022-08-17 | A ceramic nanofiber based electrochemical glucose sensor to be used as a consumable in blood sugar measurement |
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| TR2021/013445A TR2021013445A2 (en) | 2021-08-25 | 2021-08-25 | CERAMIC NANOFIBER BASED ELECTROCHEMICAL GLUCOSE SENSOR TO BE USED AS A CONSUMABLE IN BLOOD SUGAR MEASUREMENT |
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| KR102337896B1 (en) * | 2019-12-24 | 2021-12-09 | 중앙대학교 산학협력단 | Electrode of Biosensor for Glucose Detection and Method thereof |
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