TR202009267A2 - Eight Compartment Petri Box - Google Patents
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- TR202009267A2 TR202009267A2 TR2020/09267A TR202009267A TR202009267A2 TR 202009267 A2 TR202009267 A2 TR 202009267A2 TR 2020/09267 A TR2020/09267 A TR 2020/09267A TR 202009267 A TR202009267 A TR 202009267A TR 202009267 A2 TR202009267 A2 TR 202009267A2
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Abstract
Bu buluş, mikrobiyoloji ,bakteriyoloji ve biyoloji laboratuvarlarmda bakterilerin, mikroorganizmaların, mantarların kültürlenmesi için tasarlanan, bakteri üremesi için paralel şartlarda farklı testler yapmak ve bir petri kutusu içinde çeşitli ağar ,çözücü ve reaktif koşullarının incelenmesine imkan sağlayan sekiz bölmeli petri kutusu ile ilgili olup; Buluş konusu sekiz bölmeli petri kutusu (1) düz bir dairesel tabana ve silindirik bir dik duvara sahip olan bir kap ,gövde kısmı dairesel tabana ve silindirik bir dik duvara sahip olan kabın dışına uyan ve onu çevreleyen aşağıya doğru bağlı bir yan duvara sahip düz bir üstte sahip olan bir kapağı içerir. Kapak duvarı, kabın altına yakın bir şekilde uzanır.The present invention relates to an eight-compartment petri dish designed for the cultivation of bacteria, microorganisms and fungi in microbiology, bacteriology and biology laboratories, allowing different tests for bacterial growth under parallel conditions and the examination of various agar, solvent and reagent conditions in a petri dish; The eight-compartment petri dish according to the invention (1) is a vessel with a flat circular bottom and a cylindrical vertical wall, a flat top with a side wall that fits the outside of the vessel and surrounds it, the body of which has a circular bottom and a cylindrical vertical wall. Includes a cover that has. The lid wall extends close to the bottom of the container.
Description
TARIFNAME SEKIZ BÖLMELI PETRI KUTUSU Teknik Alan: Bu bulus, mikroorganizmalarin varliginin veya yoklugunun saptanmasi amaciyla , mikroorganizmalarin tanimlanmasinda esit test hacimlerine bölünmüs birden fazla örnegin ayni anda es zamanli olarak asilanmasim saglayarak karsilastirmali testlerine imkan saglayan sekiz bölmeli petri kutusu ile ilgilidir. DESCRIPTION EIGHT SECTION PETRI BOX Technical Area: This invention is used to detect the presence or absence of microorganisms, for the identification of micro-organisms in the same sample of multiple samples divided into equal test volumes. eight, which allows for the comparative tests by providing simultaneous vaccination at the same time relates to the compartmented petri dish.
Teknigin Bilinen Durumu: Günümüzde kullanilan petri kutulari , mikrobiyoloji, bakteriyoloji, immünoloji, doku kültürü, onkolojik ,virolojik yani sira gida bilimi, ilaç endüstrisi ve ilgili tüm laboratuvar çalismalarinda kültür besiyerlerinin hazirlanmasinda ve testlerinde yaygin olarak kullanilmaktadir. Uzun yillar boyunca, en yaygin kullanilan petri kutulari, tipik olarak camdan yapilmistir. Cam petri kutulari sterilize edilerek yeniden kullanilabiliyorken ,cam petri kutularinin üretim ve kullanimi sirasinda kolayca çatlayabilmesi, kirilabilmesi, içerilerinde bulunan steril besiyerin kontamine olmasindan ya da içerisinde üretilmis bakterilerin çevreye bulasabilmesinden dolayi kullanimi azalmistir. State of the Art: Petri dishes used today are used in microbiology, bacteriology, immunology, tissue culture, oncological, virological as well as food science, pharmaceutical industry and all related laboratory studies It is widely used in the preparation and testing of culture media. Long years Throughout, the most common petri dishes were typically made of glass. glass petri dishes While they can be sterilized and reused, the production and use of glass petri dishes easily cracked or broken during It is not used because the bacteria produced in it can contaminate the environment. has decreased.
Genel olarak, kullanilan petri kutularinda, büyüme spektrumu ölçümü, mikroorganizmalari etkileyen kimyasal faktörler ve tür seçimi gibi deneylerde tararken ve tanimlarken, ayni alandaki islemden dolayi çogu zaman birçok problemler meydana gelir.Büyüme spektrumunu ölçerken, islem biraz istem disidir ve büyüme membranlari genellikle birbirleriyle bitisiktir, böylece hangi büyüme faktörünün türün büyümesini etkiledigine karar vermek iinkansizdir.Sonuçta ortaya çikan inhibisyon zonu veya saydam çemberin bozulmasi, birbiriyle etkilesime girer, çapini tam olarak belirleyemez, sonuçta testin tekrar edilmesi gerekir. Tür seçimi sirasinda çok sayida kültür kabina ihtiyaç duyulur ve temizleme ve sterilizasyon isi çok zaman almaktadir. In general, in the petri dishes used, growth spectrum measurement, microorganisms while scanning and identifying in experiments such as chemical factors affecting species selection and Many problems occur most of the time because of the operation in the field. when measuring, the process is somewhat involuntary and the growth membranes are usually adjacent to each other, thus deciding which growth factor influences the growth of the species. It is impossible. The resulting inhibition zone or the disruption of the transparent circle, interacts, cannot determine its diameter exactly, as a result, the test must be repeated. Type During the selection, a large number of culture cabinets are needed and the cleaning and sterilization work is very it takes time.
Geleneksel bir petri kutusu, bir seferde sadece bir mikroorganizmayi kültürleye bilir, çünkü petri kutusunun iç alaninda bölünme araligi yoktur. Çogu zaman geleneksel petri kutulari düsük bir kullanim alanina sahiptir ve ortamin yüzeyi mikroorganizmalari kültürlemek veya izole etmek için sadece küçük bir alana müsaade ederler ve diger alanlar tamamen yararsizdir.Rutin testlerde ve bilinmeyen türlerin tanimlanmasinda özellikleri belirlemek için çoklu testlerde yapilmasi gerekebilmektedir. A conventional petri dish can only culture one microorganism at a time because There is no division gap in the inner area of the petri dish. Oftentimes, conventional petri dishes are low. It has a field of use and the surface of the medium is used to culture or isolate microorganisms. They only allow a small area to perform and the other areas are completely useless. in tests and in multiple tests to identify features in the identification of unknown species. may need to be done.
Günümüzde mikrobiyolojik ve bakteriyolojik analizlerde kültür elde etmek için 150 mm çapindaki petri kutulari içinde bulunan besiyeri yüzeyine elle manuel olarak veya bir cihaz vasitasi ile otomatik olarak mikrobiyal ekim yapilmaktadir.Petri kutusunda kullanilan besiyerler (besi ortami, ortam, vasat, kültür ortami, kültür vasati, kültür besiyeri) mikroorganizmalarin gelistirilmesi için formülize edilmis ortamlardir. Today, 150 mm is used to obtain cultures for microbiological and bacteriological analysis. manually or by a device on the surface of the medium in petri dishes. Microbial sowing is done automatically by means of media (medium, medium, medium, culture medium, culture medium, culture medium) are media formulated for the growth of microorganisms.
Bunlar,mikroorganizmalarin gelistirilmesi, izolasyon, identiiikasyon, sayim, duyarlilik testleri, sterilite testleri, klinik örneklerin incelenmesi, gida, su ve çevre kontrolleri, biyolojik ürünlerin elde edilmesi, antibiyotik ve vitamin analizleri, endüstriyel analizler vb. gibi çok farkli amaçlara yönelik olarakta kullanilabilmektedir. These are the development of microorganisms, isolation, identification, enumeration, susceptibility tests, sterility tests, examination of clinical samples, food, water and environmental controls, biological products obtaining, antibiotic and vitamin analysis, industrial analysis, etc. for different purposes such as can also be used for
Petri kutulari mikrobiyoloji çalismalarinda genellikle agar plakalari yapmak için kullanilir. Petri dishes are often used to make agar plates in microbiology studies.
Sicak sivi içeren agar kabi kismen besin maddeleri, kan, tuz, karbohidratlar, indikatörler, boyalar, amino asitler ve anti biyotikler gibi belirli maddelerin karisimini içeren içerik ile doldurulur. Daha sonra agar sogur ve kati lasir. Artik plaka asilama olarak bilinen süreçte, Örnek almaya uygun hale gelir. Virüs veya faj kültürler için, iki asamali bir asilama gereklidir. The agar dish containing hot liquid is partially composed of nutrients, blood, salt, carbohydrates, indicators, with ingredients containing a mixture of certain substances such as dyes, amino acids and antibiotics is filled. Then the agar is cooled and solidified. In the process now known as plate etching, Example becomes eligible to receive. For virus or phage cultures, a two-stage inoculation is required.
Bakteriler ilk ana viral inokülümü saglamak üzere yetistirilmektedir. Petri kutularindaki agar, havadaki partiküllerin dibe çökerek kültürlenmis mikroplara bulasma riskini azaltmak amaciyla ve ortami bozan su yogunlasmasini önlemek için bas asagiya döndürülerek inkübe edilir. Bilim adamlari uzun dogal hücrelerin yetistirilmesi için geleneksel sert yüzeylerin uygun olmadigini ve bu tür fenotipleri ortaya çikartmak için matris yapay yumusak ortamlar olusturulmasi gerektigini dile getirmektedirler. Ancak yumusak bir bir matris içerisinde hücre büyütülmesi yogun emek gerektiren, oldukça pahali ve pratik olmayan bir girisimdir. Bacteria are bred to provide the first major viral inoculum. agar in petri dishes, to reduce the risk of airborne particles sinking to the bottom and contaminating cultured microbes. and incubated by turning the head down to prevent water condensation, which spoils the environment. Science They said that traditional hard surfaces are not suitable for growing long natural cells. and the creation of matrix artificial soft environments to elicit such phenotypes. they state that it is necessary. However, cell growth in a soft matrix It is a labor-intensive, expensive and impractical undertaking.
Klinik kullanimlarinin disinda petri kutulari ayni zamanda sivi bir ortamda veya kati agar kullanilarak, ökaryotik hücre kültürü için de kullanilabilir. Bos Petri kutulari bitki çimlenmesi veya Hayvan etlerinin, kümes hayvanlarinin, yumurtalarin ve bunlarin islemlerinin ürünlerinin mikrobiyolojik kontrolü ve küçük hayvan davranislarinin incelenmesi ya da bu tür örnekleri firinda kurutma, tasima veya depolama amaciyla gün geçtikçe kullanim alani artan çesitli Mevcut bulus prensibine ait ilk uygulama örnegi SEKIL:5 ve SEKIL:6“da tasvir edilmistir.Acinetobacter cinsi Moraxellaceae familyasina ait bir bakteridirDünya çapinda degisik merkezlerde nozokomiyal salginlara yol açmakta ve siklikla çoklu antibiyotik direnci göstermektedir. Rutin mikrobiyoji laboratuvarlarinda otomatik bakteri identifikasyon sistemlerinin yanisira manuel olarak antibiyogram testi uygulanmaktadir. Apart from clinical uses, petri dishes can also be used in a liquid medium or on solid agar. It can also be used for eukaryotic cell culture. Empty Petri dishes plant germination or the products of animal meats, poultry, eggs and their processing microbiological control and study of small animal behavior or examples of such It is used for drying in the oven, for transportation or storage. The first application example of the present invention principle is depicted in FIG. 5 and FIG. 6 The genus Acinetobacter is a bacterium belonging to the Moraxellaceae family. It causes nosocomial outbreaks in different centers and is frequently multi-antibiotic resistance. shows. Automatic bacterial identification in routine microbiology laboratories In addition to the systems, manual antibiogram test is applied.
Tasvir edilen ilk uygulama örneginde Colistin adli antibiyotik çoklu ilaç direnci olan olgularda son tercihdir.Fakat etkili bir ajan olarak denenmektedir. Son yillardaki uygulamalarda otomatik cihaz sonuçlarindaki bazi süpheli sonuçlar manuel mikrodilüsyon ile yapilan colistin duyarlilik çalismalarini rutine sokmus ancak pahali olmasi dezavantaja neden olmustur. Mikrodilusyon üreme duyarlilik/direnç degerlendirilmesi yapilmaktadir. 2 mikrogram/ml ve altindaki üreme inhibisyonu colistin isimli antibiyotigin bakteriye etkili oldugunu göstermektedirRutinde ise tüm antibiyotikler otomatik yada manuel (mueller-hinton agar vb.besiyeri dökülmüs petri kutularinda) bakteriye karsi denenmektedir. In the first application example depicted, Colistin forensic antibiotic was used in cases with multidrug resistance. It is the last choice. But it is being tested as an effective agent. Automatically in applications in recent years some questionable results in instrument results colistin sensitivity with manual microdilution He put his work into a routine, but it was a disadvantage because it was expensive. Microdilution reproductive susceptibility/resistance evaluation is performed. Reproduction of 2 micrograms/ml and below Its inhibition shows that the antibiotic named colistin is effective against bacteria. all antibiotics are automatic or manual (mueller-hinton agar etc. boxes) are tested against bacteria.
Bu bulus, yukarda belirtilen dezavantajlarin üstesinden gelebilen Ortak bir ortamda organizmalarla es zamanli çalismalar için tasarlanmis sekiz bölmeli petri kutusu olup; Her bir bölmeye ayri ayri olarak petri kutusuna dökülmüs antibiyogram agar besiyerinde her bir bölmede mikrodilüsyon yöntemindekilerle ayni yogunlukta colistin içeren besiyeri (0.125, saptanmasi saglanmis ve ayrica diger eksiklikler ortadan kaldirilarak testlerin dogrulugu ispatlanmistir. Karsilastirmali ölçümler/kosullar için bölünmüs besiyer veya farkli agar besiyerlerinin uygulanmasinada imkan saglamistir.Ayrica farkli ortamlarda kullanim için veya inkübatör alanindada tasarruf gerektiginde ideal oldugu görülmüstür. This invention can be found in a Common environment that can overcome the above mentioned disadvantages. It is an eight-compartment petri dish designed for simultaneous studies with organisms; Each Each cell in antibiogram agar medium poured into petri dishes separately medium containing colistin at the same concentration as in the microdilution method in the chamber (0.125, detection and accuracy of the tests by eliminating other deficiencies. has been proven. Split media or different agar for comparative measurements/conditions It also allows the application of media. Also for use in different environments or It has been found to be ideal when saving in incubator space is required.
US4042463A sayili Birlesik devletler patent dökümaninda Mikroorganizmalarin transferi, ekimi, bakimi ve tanimlanmasi için çoklu bir asilama sisteminden bahsedilmektedir. Bu dökümanda bahsedilen bulus, bir çoklu igne asilama plakasi, inokulumu tutmak ve ignelere aktarmak için ignelere karsilik gelen çoklu oyuklar içeren ayri bir plaka ve besiyerin orta kisminda birbiriyle baglantili çok sayida bölmeye sahip bir petri kutusundan bahsedilmektedir.Bu bulusta birbirinden bagimsiz bölme bulunmamaktadir. Transfer of microorganisms in the United States patent document US4042463A, A multiple grafting system is mentioned for its cultivation, maintenance and identification. This The invention described in the document is a multi-needle grafting plate to hold the inoculum and attach it to the needles. a separate plate containing multiple wells corresponding to the needles and the middle of the medium for transfer from a petri dish with several interconnected compartments in the There are no independent divisions in this invention.
US3902972A sayili Birlesik devletler patent dökümaninda otomatik asilama için modifiye edilmis petri kabindan bahsedilmektedir. Bu dökümanda bahsedilen bulus gram pozitif ve gram negatif bakteri karisimlarini ayirarak, organizmalarin klinik örneklerden izole edilmesini kolaylastiran ve saf kültürlerin koloni büyüme özelliklerini karsilastirmak için bölmelere sahiptir. Bu bulusta birbirinden bagimsiz bölme bulunmamaktadir. Modified for automatic grafting in United States patent document US3902972A It is mentioned in a petri dish. The invention mentioned in this document is gram positive and gram isolation of organisms from clinical specimens by separating mixtures of negative bacteria. compartments to compare colony growth characteristics of facilitating and pure cultures. has. There are no independent divisions in this invention.
Teknigin bilinen durumunda ,testlerin ayri ayri petri kutularinda yapilinasi yaygindir. Son zamanlarda istiflenebilir ve kaygan petri kutularinin iki bölmeli, üç bölmeli ve dört bölmeli olarak benzerlerinin kullanildigi görülmektedir.Fakat sekiz bölmeli petri kutusu henüz bulunmamaktadir. In the state of the art, it is common for tests to be performed in separate petri dishes. End Two-compartment, three-compartment and four-compartment petri dishes are stackable and slippery at times. It is seen that similar ones are used as does not exist.
Sonuç olarak, yukarda bahsedilen tüm sorunlar,ilgili teknik alanda bir yenilik yapmayi zorunlu hale getirmistir. Mevcut bulus, teknikte bu ihtiyaci ve istegi yerine getirir ve yeni bir hücre kültür sistemi saglar. As a result, all the above-mentioned problems compel an innovation in the relevant technical field. does not make it. The present invention fulfills this need and desire in the art and creates a new cell. culture system.
Bulusun Kisa Açiklanmasi: Mevcut bulus, yukarda bahsedilen gereksinimleri karsilayan, tüm dezavantajlari ortadan kaldiran ve ilave bazi avantajlar getiren sekiz bölmeli petri kutusu ile ilgilidir. Brief Description of the Invention: The present invention satisfies the above-mentioned requirements, avoiding all the disadvantages. It is related to the eight-compartment petri dish, which lifts and brings some additional advantages.
Bu bulusun amaci, Mikrobiyoloji, bakteriyoloji ve biyoloji deneylerinde organizma ve kültür yetistirmek için iç bölme duvarlari arasinda hava akisina imkan vererek birden fazla örnegin ayni anda es zamanli olarak asilanmasina ve karsilastirmali testlerine imkan saglayan gelismis birtek kullanimlik sekiz bölmeli petri kutusu (1)”nun gerçeklestirilmesidir. The aim of this invention is to study organism and culture in microbiology, bacteriology and biology experiments. multiple samples by allowing air flow between the interior partition walls to grow advanced simultaneous vaccination and comparative testing is the realization of a disposable eight-compartment petri dish (1).
Mevcut bulusun ilave bir amaci, anaerobik büyümeye elverisli kosullar yaratarak için petri kutusu içindeki atmosferin kimyasal reaksiyon ile islenmesi için, ayri ayri besiyerlerin döküldügü birkaç ayri alt bölmeye sahip anaerobik kültürleme cihazi saglamaktir. An additional aim of the present invention is to create conditions conducive to anaerobic growth. In order to treat the atmosphere in the box with a chemical reaction, it is necessary to prepare the media separately. is to provide an anaerobic culturing device with several separate sub-chambers into which it is poured.
Bu bulusun baska bir amaci , bakterilerin üremesi için gerekli olan fiziksel sartlarin siralanmasi, mikrobiyal ekim islemlerinin daha basit ve ekonomik bir sistem ile standart bir sekilde yapilmasi ve essiz dairesel düz taban yapisi sayesinde tek tip hücre büyümesini saglayarak, Sirali olarak besiyer derinliginin petri kutusunun her alaninda esit olmasinin gerçeklestirilmesidir. Another purpose of this invention is to enumerate the physical conditions necessary for the growth of bacteria, microbial cultivation processes in a standard way with a simpler and more economical system. by providing uniform cell growth thanks to its unique circular flat base structure, Make sure that the depth of the medium is equal in all areas of the petri dish, respectively. is realization.
Bu bulusun baska bir amaci , mikrobiyal ekim islemlerinde sterilize edilen petri kutularinda bir sonraki deneyde çapraz kirlilik bir sorun haline gelebileceginden dolayi, steril olmayan sekiz bölmeli petri kutusu sayesinde çok sayida ayri ayri petri kutusu kullanmak gerekli olmadigindan çapraz kontaminasyon riskinin ortadan kaldirmasi gerçeklestirilmistir. Another object of this invention is to provide a sterile solution in petri dishes that are sterilized in microbial cultivation processes. Since cross-contamination may become a problem in the next experiment, eight non-sterile Thanks to the divided petri dishes, it is necessary to use many separate petri dishes. Since there is no cross contamination, the risk of cross contamination has been eliminated.
Bu bulusun baska bir amaci , ayni petri kutusu içine farkli besiyer ve çözücü malzemeleri ayri bölmelere yerlestirilerek farkli reaktiflerle ayri ayri muamele edilmesine izin vererek mikroorganizmalarin üreme hizlari ve dönemlerini tanimlama , farkli tipte hücrelerin kültürlenmesini ve tanimlanmis alanlarda çalisilmasinin gerçeklestirilmesidir. Another purpose of this invention is to separate different media and solvent materials in the same petri dish. by placing them in chambers, allowing them to be treated separately with different reagents. describing the reproduction rates and periods of microorganisms, different types of cells culturing and working in defined areas.
Bu bulusun baska bir amaci, iç bölme duvarlarinin besiyer üzerinde gelisen bir hücre tipinin diger bölmelerdeki hücrelerle etkilesime girmesine ve bulasmasina izin vermeyen dairesel tabanin merkezinde konumlanmasi, inkibasyon asamasinda yogusmayi azaltan ve bölmeler arasinda hava sirkülasyonunu saglayan havalandirma kanalina sahip olan bir yapida havalandirmanin gerçeklestirilmesidir. Another object of the present invention is to ensure that the inner partition walls of a cell type grown on the medium are circular, which does not allow it to interact and contaminate cells in other compartments. positioning the sole in the center, reducing condensation during the incubation phase and separating in a structure that has a ventilation channel that provides air circulation between the realization of ventilation.
Bu bulusun baska bir amaci , Hücre etkilesimlerinin izlenmesi , etkilesen hücreler üzerinde çesitli analizler yapmak kolaydir, çünkü hücresel etkilesimler sadece tanimlanmis alanlarda, yani petri kutusunun bosluk alanlarinda, iç bölme duvarlarinin sagladigi alanlarda gerçeklestirilmesidir. Another purpose of this invention is to monitor cell interactions, to monitor the interacting cells. it is easy to perform various analyzes because cellular interactions only occur in defined areas, that is, in the empty areas of the petri dish, in the areas provided by the inner partition walls is realization.
Bu bulusun baska bir amaci , Mikrobiyoloji, bakteriyoloji ve benzer hücre kültürü Çalismalarinda kullanilmak üzere son derece seffaf renksiz ve renklendirilmis polistirenden üretilirler. Kapak gövde üzerine tam uyumlu olup devamli ve efektif çalismayi saglar. Ideal gövde yapisi otomatik dolum makinalari ile tam uyumlu çalismaya uygundur. Another object of this invention is microbiology, bacteriology and similar cell culture. Made of extremely transparent, colorless and colored polystyrene for use in its work. they are produced. The cover is fully compatible with the body and ensures continuous and effective operation. Ideal body structure is suitable for working in full harmony with automatic filling machines.
Bulusun yine bir baska amaci, eger plaklar diskler yerlestirilmeden önce, uzun süre oda isisinda birakilirsa organizma üremeye baslayabilir, bu durum inhibisyon zon Ölçülerinde hatali azalmayla sonuçlanir. Diskler, agarin yüzeyine ekim yapildiktan sonra 15 dk içinde yerlestirilmelidir. Anaerobik kültürleme için ortamin son derece hizli ve oldukça verimli bir sekilde hazirlanmasina imkan saglayan sekiz bölmeli petri kutusu sayesinde testie hazirlik süresinin kisaltilmasi ?petri kutusu sarfiyatinin azaltilmasi ve test maliyetlerinin düsürülmesinin gerçeklestirilmesidir. Yet another object of the invention is that if the platters are stored at room temperature for a long time before the discs are inserted. If left untreated, the organism may begin to reproduce, which may result in incorrect inhibition zone measurements. results in a decrease. Discs should be harvested within 15 minutes of sowing on the agarine surface. should be placed. Extremely fast and highly efficient removal of the medium for anaerobic culturing. Preparation for the jug thanks to the eight-compartment petri dish that allows it to be prepared shortening the duration of ?reducing petri dish consumption and reducing test costs is realization.
Bulusun baska bir amaci,besiyeri yüzeyindeki agar üzerine tek koloniden yogun koloniye kadar farkli asamalarda ekiin yapilarak ihtiyaç duyulan maksimum koloni izolasyonunu saglamaktir. Another object of the invention is to work from single colonies to dense colonies on agar on the surface of the medium. It is to provide the required maximum colony isolation by planting at different stages.
Bulusun bir baska ve daha spesifik amaci; sekiz bölmeli petri kutusuna dökülmüs agarli besiyerleri sürme , yayma ve dökme plak yöntemlerinden birisinin veya ayri ayri bölmelerde her birisinin kullanilarak ekim yapilabilmesi ve inkübasyona tabi tutulmasinin gerçeklestirilmesidir. Another and more specific object of the invention is; agarli poured into an eight-compartment petri dish The media can be used in one of the smear, spread and pour plate methods or in separate compartments. sowing and incubation using each is realization.
Bulusun bir baska ve daha spesifik amaci; petri kutulari genellikle agar plakalari yapmak için kullanilir. Agar plakasi kismen sicak sivi ihtiva eden agar besiyeri, kan, tuzlar, karbonhidratlar, boyalar, göstergeler, amino asitler ya da antibiyotikler içerebilen bilesenlerin karisimi ile doldurulabilmektedir.Nadirde olsa,bazi bakteriler asit ortamlarda,bazilar1 ise bazik ortamlarda daha iyi ürerler. Sekiz bölmeli petri kutusu“nun kimyasallara dayanikli yapisi sayesinde çesitli testlerinde yapilabilirliginin gerçeklestirilmesidir. Another and more specific object of the invention is; Petri dishes are often used to make agar plates. used. Agar plate is agar medium containing partially hot liquid, blood, salts, carbohydrates, with a mixture of ingredients that may include dyes, indicators, amino acids or antibiotics Although rare, some bacteria are in acid environments, and some in basic environments. they breed better. Thanks to the chemical-resistant structure of the eight-compartment petri dish, various realization of feasibility in tests.
Bulusun yapisal ve karakteristik özellikleri ve tüm avantajlari asagida verilen sekiller ve bu sekillere atiflar yapilmak suretiyle yazilan detayli açiklama sayesinde daha net olarak anlasilacaktir ve bu nedenle degerlendirmenin de bu sekiller ve detayli açiklama göz önüne alinarak yapilmasi gerekmektedir. The structural and characteristic features of the invention and all its advantages are given in the following figures and More clearly thanks to the detailed explanation with references to the figures. will be understood and therefore the assessment should also take into account these figures and detailed explanation. must be made by taking
Sekillerin Açiklanmasi: Bulusun amacina ulasmak için gerçeklestirilen sekiz bölmeli Petri kutusu ekli sekillerde gösterilmis olup, bu sekillerden; SEKILel Sekiz Bölmeli Petri kutusu genel görünümüdür. Explanation of Figures: In order to achieve the aim of the invention, the eight-compartment Petri dish is in the attached figures. has been shown, from these figures; FIGURE This is the general view of the Eight Compartment Petri dish.
SEKIL-Z Sekiz Bölmeli Petri kutusunun kesit halindeki önden görünümüdür. FIGURE-Z is the cross-sectional front view of the Eight Compartment Petri dish.
SEKILIB Sekiz Bölmeli Petri Kutusu iç bölme duvarli ve isaretli görünümüdür. SEKILIB Eight Compartment Petri dish is a marked view with an inner partition wall.
SEKILI4 Sekiz Bölmeli Petri Kutusu, çizgi ekim yöntemi görünümüdür. SEKILI4 Eight-Compartment Petri dish is the line cultivation method view.
SEKIL-S Sekiz Bölmeli Petri Kutusu,damla plak ekim yöntemi görünümüdür. FIGURE-S Eight Compartment Petri dish, drop plate sowing method view.
SEKILýö Sekiz Bölmeli Petri Kutusu,üst üste istifleme görünümüdür. SEKILIO Eight Compartment Petri dish is the stacking view.
Sekillerdeki Referanslarin Açiklanmasi: Bu bulusun anlasilmasina yardimci olacak sekiller ekli resimde belirtildigi gibi numaralandirilmis olup, bu numaralarin karsiligi isimleri ile beraber asagida verilmistir. 1. Sekiz Bölmeli Petri kutusu 2. Üst Kapagi 3. Alt Tabagi 4. Dairesel Alt Taban . Dis Duvar 6. Iç Bölme Duvari 7. Istifleme Yuvasi 8. Istifleme Halkasi 9. MH Agar Besiyeri . Kabartma Bölme Sayisi 12. Petri Kutusu Merkezi 13. Halka Öze Aleti 14. Çizgi Ekim . Antibiyotik Diski 16. Inhibisyon Zonu 17. Etkili Antibiyotik Bu ayrintili açiklamada,bulus konusu sekiz bölmeli petri kutusu (1) ve tercih edilen yapilanmalari,sadece konunun daha iyi anlasilmasina yönelik olarak ve hiçbir sinirlayici etki olusturmayacak sekilde açiklanmaktadir. Seki1:1,2,3,4,5 ve 6'da bulus konusu sekiz bölmeli petri kutusu (1)”nun görünümleri verilmektedir. Explanation of References in Figures: Figures to assist in understanding this invention are as indicated in the attached picture. are numbered and the equivalents of these numbers are given below along with their names. 1. Eight-Compartment Petri dish 2. Top Cover 3. Bottom Plate 4. Circular Bottom Base . Outer wall 6. Interior Partition Wall 7. Stacking Slot 8. Stacking Ring 9. MH Agar Medium . Number of Relief Sections 12. Petri Box Center 13. Ring Loop Tool 14. Line October . Antibiotic Disc 16. Zone of Inhibition 17. Effective Antibiotic In this detailed description, the subject of the invention is the eight-compartment petri dish (1) and the preferred structuring is only for a better understanding of the subject and has no limiting effect. described in a way that does not constitute. In Figs1:1,2,3,4,5 and 6, the subject of the invention has eight compartments. The views of the petri dish (1) are given.
Bulus konusu sekiz bölmeli petri kutusu (l) sunlari içerir ; SEKlel “de gösterildigi gibi Sekiz bölmeli petri kutusu (l) standart boyutta, iki sekliyle ayni, ancak büyüklük kisimlarinda farkli çevresinden asagiya sarkan bir bir dis duvar (5) “dan olusmaktadir. SEKIL 3'te gösterilen Alt Tabagi (3)`nin dairesel alt taban (4)”ina bitisik içinde Iç Bölme Duvari (6) ile birbirinden ayrilmis olan böylece her biri farkli sekiz kültür ortami olusturan, birbirlerinden esit aralikli Ölçülerde bölmelerin kaçinci bölme oldugunun daha kolay tanimlanmasini saglayan Kabartma Bölme Sayisi (10) sayisal isaretlerle, ve alt tabagi (3)”nin Dis Duvar (5)'inda yer alan cetvel formundaki Sivi kültür Seviye Cetveli (1 1)'ni içerrnektedir. The subject of the invention is the eight-compartment petri dish (1); Eight as shown in SEKlel compartment petri dish (l) standard size, same as two, but different in size portions It consists of an outer wall (5) hanging down from its periphery. Bottom shown in FIG. Inner Partition Wall (6) adjacent to the circular bottom base (4) of the Plate (3) and separated from each other. equidistant from each other, thus forming eight different culture media, each Relief, which makes it easier to define which division is the division in the dimensions. Number of Partitions (10) with numeric signs, and the ruler located on the Outer Wall (5) of the bottom plate (3) It contains the Liquid Culture Level Chart (1 1) in the form.
Bulus konusu sekiz bölmeli petri kutusu (l)°nun SEKIL22 “de enine kesit görünüsüdür. Üst kapagi (2) ve alt tabagi (3) arasinda sizdirmazligi göstermek amaciyla. The subject of the invention is the cross-sectional view of the eight-compartment petri dish (1) in FIG.22. Top to show the tightness between the cover (2) and the bottom plate (3).
Bulus konusu Sekiz Bölmeli Petri kutusu (1)”nun SEKIL 3'te gösterilen Alt Tabagi (3) içindeki Iç Bölme Duvari (6) bulustan ayrilmadan çesitli sekillerde, boyutlarda ve sayilarda olabilir; ve ayrica her biri Petri Kutusu Merkezi (12)7ninde birleserek, Iç Bölme Duvari (6) ile birbirinden ayrilmis olan böylece her biri farkli bir kültür ortami olusturan bölmelerin kaçinci bölme oldugunun daha kolay tanimlanmasini saglayan, Dairesel alt taban (4) içinde birbirlerinden esit aralikli ölçülerde Kabartma Bölme Sayisi (10) sayisal isaretlerle yine Alt Tabagi (3) sivi kültürün döküldügü mikroorganizma büyüme ortami içerir ve Alt Tabagi (3) içindeki her bir bölmeye ayri ayri ölçü ve ölçekte sicak sivi ihtiva eden agar, besin, kan, tuzlar, kabonhidratlar, boyalar, amino asitler ya da antibiyotikler içeren belirli bilesenlerin karsimi ile doldurulur. Alt Tabagi (3) Dis Duvar (5)”inda yer alan isaret bir cetvel formunda karakterize olup, sivi kültür Seviye Cetveli (1 1) olarak islev görmesini saglamaktadir. The subject of the invention is in the Subplate (3) of the Eight Compartment Petri dish (1), shown in FIG. The Inner Partition Wall (6) may be of various shapes, sizes and numbers without departing from the invention; and also, each of them converging in the Petri-Box Center (12), separated from each other by the Inner Partition Wall (6). which section of the sections that are separated so that each of them creates a different culture medium Equal to each other within the Circular subbase (4), which makes it easier to identify Relief Section Number (10) in intermittent sizes, again with numeric signs, Substrate (3) liquid The microorganism on which the culture is poured contains growth medium and each individual in the Substrate (3) agar containing hot liquid, food, blood, salts, carbohydrates, The dyes are filled with a mixture of certain ingredients containing amino acids or antibiotics. Lower The sign on the outer wall (5) of the Plate (3) is characterized in the form of a ruler, and the liquid culture It allows it to function as a Level Chart (1 1).
Bulus konusu Sekiz Bölmeli Petri kutusu (1)”nun kullanim alanina göre, SEKIL 4'te gösterilen Çizgi Ekim (14) Yöntemi ile Iç Bölme Duvari (6) ile birbirinden ayrilmis bölmelerindeki ,MH Agar Besiyeri (9),nin farkli bölgelerine örnek ile temas ettirilen Halka Öze Aleti (13) sirasiyla sürülür. Örnek miktari ve içerdigi mikroorganizma sayisinin her bölgede farkli olmasi nedeniyle üreme miktarlarinin da farkli olmasi saglanir. Her bir Iç Bölme Duvari (6) ile birbirinden izole edilmis bölmelerdeki numunelerin analiz edilmesi saglanmaktadir. According to the usage area of the Eight Compartment Petri dish (1), which is the subject of the invention, as shown in FIG. MH in the partitions separated from each other by the Line Sowing (14) Method and the Inner Partition Wall (6). Ring Loop Tool (13), which was contacted with the sample on different parts of the Agar Medium (9), respectively. is driven. The amount of sample and the number of microorganisms it contains are different in each region. Due to this reason, it is ensured that the reproduction amounts are also different. With each Interior Partition Wall (6) It is provided to analyze the samples in the compartments that are isolated from each other.
Bulus konusu Sekiz Bölmeli Petri kutusu (l),nun bir baska kullanim alanina göre, SEKIL 5'te gösterilen damla plak ekim yöntemi ile Iç Bölme Duvari (6) ile birbirinden ayrilmis bölmelerine dökülmüs olan, MH Agar Besiyeri (9)`nin üstüne farkli miktarlarda ekilen Antibiyotik Diski (15)”nin MH Agar Besiyeri (9) üzerindeki Etkili Antibiyotik (17) diskleri ve etkisiz Antibiyotik Diski (15) gözlemlenerek olusturduklari Inhibisyon Zonu (16) ölçümlerine göre, örnek miktari ve içerdigi mikroorganizma sayisinin her bölmede farkli olmasi nedeniyle üreme miktarlarinin Bulus konusu Sekiz Bölmeli Petri kutusu (1),nun SEKIL 6'da görüldügü gibi üst üste ve alt alta istiflenebilmesini ve dengelenmesini saglayabilmek için üst ve alt istifleme halkasina sahiptir. According to another usage area of the Eight Compartment Petri dish (l), the subject of the invention is shown in FIG. with the drop plate sowing method shown, into the partitions separated by the Inner Partition Wall (6). Antibiotic Disc sown in different quantities on top of spilled MH Agar Medium (9) Effective Antibiotic discs of (15) on MH Agar Medium (9) and ineffective Antibiotic According to the Inhibition Zone (16) measurements formed by observing the disk (15), the sample amount and the number of microorganisms it contains is different in each compartment. The subject of the invention is the Eight Compartment Petri dish (1), one above the other and one below the other, as seen in FIG. It has upper and lower stacking rings to enable it to be stacked and balanced.
Genel laboratuvar kullanimi sirasinda inkübatörlerde ve depolamada yerden tasarruf saglamak amaciyla ve kullaniinlarini daha pratik hale getirmek için özel olarak tasarlanmistir. Sekiz Bölmeli Petri kutusu (1)'nun istiflenebilir ve otomatik dolum sistemleri için gelistirilmis kaygan petri kutusuda mevcuttur.Öze11ikle kaygan Sekiz Bölmeli Petri kutusu (1), Otomatik dolum ve çizgi sistemleri için tasarlanmistir.Sekiz Bölmeli Petri kutusu (1)”nun rahat ve tam oturmasini saglayan simetrik paralel istifleme halkasi (8) ve Istifleme yuvasi (7)”1 sayesinde Sekiz Bölmeli Petri kutusu (1)'nun güvenli ve stabil istiflenmesi saglanmaktadir. To save space in incubators and storage during general laboratory use It has been specially designed for the purpose and to make its use more practical. Eight Developed for stackable and automatic filling systems of compartment Petri dish (1) Also available in a slippery Petri dish. Particularly slippery Eight Compartment Petri dish (1), Automatic It is designed for filling and line systems. thanks to the symmetrical parallel stacking ring (8) and the Stacking slot (7)”1. Safe and stable stacking of the Eight Compartment Petri dish (1) is ensured.
Bulusun Sanayiye Uygulanma Biçimi: Yukarda bahsedilen amaçlara hizmet eden Sekiz bölmeli petri kutusunun, iç pazar ve dis pazarda ,mikrobiyoloji laboratuvarlarinda sikça ve düzenli olarak kullanim zorunlulugu bulunan mevcut iki bölmeli, üç bölmeli ve dört bölmeli petri kutularinin yerine gerek spesifikasyonunun daha yüksek olusu, gerekse kullanicilar için kullanim kolayligi ve test maliyetlerini oldukça asagiya çekmesi sebebiyle sanayinin herhangi bir dalinda üretilebilir ve kullanilabilir nitelikte olup sanayiye uygulanabilir yapidadir. Industrial Application of the Invention: Eight-compartment petri dish serving the above-mentioned purposes, domestic market and foreign frequent and regular use in the market, microbiology laboratories No need to replace existing two-compartment, three-compartment and four-compartment petri dishes. higher specification, as well as ease of use for users and testing It can be produced in any branch of the industry due to the fact that it reduces the costs considerably and It is usable and industrially applicable.
Tanimlamada Belirtilen Referanslar Basvuran tarafindan belirtilen bu referanslar listesi yalnizca okuyucu için bir kolaylik saglamasi içindir. Avrupa patent dokümaninin bir parçasini teskil etmez.Referanslarin derlenmesinde büyük bir özen gösterilmis olmakla birlikte hatalar ve eksikler olabilir ve EPO bu anlamda hiçbir sorumluluk üstlenmemektedir. References Specified in the Description This list of references cited by the applicant is for the reader's convenience only. is for. It does not form part of the European patent document. Whilst great care has been taken, errors and omissions may occur and the EPO is therefore assumes no responsibility.
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