TR201816265T4

TR201816265T4 - Combinations of biological control agents and fungicides.

Info

Publication number: TR201816265T4
Application number: TR2018/16265T
Authority: TR
Inventors: Wolfram Andersch Dr; Hawen Evans Paul; Bernd Springer Dr; Bugg Kevin; Riggs Jennifer; Roy Chen Chi̇-Yu
Original assignee: Bayer Cropscience Ag
Priority date: 2008-04-07
Filing date: 2009-04-07
Publication date: 2018-11-21
Also published as: HUE042281T2; PT2918172T; MX361213B

Abstract

Bacillus firmus CNCM 1-1582 sporu ve bir fungisit içeren bir kompozisyon olup, özelliği; burada söz konusu fungisitin fluopiram, fluoksastrobin, fludioksonil, iprodion, mankozeb'den seçilmesidir.A composition comprising Bacillus firmus CNCM 1-1582 spore and a fungicide. wherein said fungicide is selected from fluopyram, fluoxastrobin, fludioxonil, iprodion, mankozeb.

Description

TARIFNAME BIYOLOJIK KONTROL AJANLARI VE FUNGISIT KOMBINASYONLARI Mevcut bulus genel olarak bitki sagligindaki, canliligindaki ve verimindeki bitki parazitik nematod ve mantarlardan kaynaklanan genel hasar ve kayiplari azaltmaya yönelik kompozisyonlar ve yöntemlerle ilgilidir. Daha spesifik olarak, mevcut bulus, tarimsal olarak yararli en az bir biyolojik kontrol ajani ve en az bir böcek kontrol ajani içeren kompozisyonlarla ve ayni zamanda bitkileri ve bitki materyalini tedavi etmek için bu kompozisyonlardan yararlanan yöntemlerle ilgilidir. DESCRIPTION BIOLOGICAL CONTROL AGENTS AND FUNGITIDE COMBINATIONS The present invention generally improves plant health, vitality and yield. aimed at reducing overall damage and loss from nematodes and fungi. relates to compositions and methods. More specifically, the present invention containing at least one useful biological control agent and at least one insect control agent with compositions but also for treating plants and plant material relates to methods that make use of compositions.

Nematodlar hemen hemen her türlü ortamda (kara, tatli su, deniz) bulundugu bilinen mikroskobik bölümlere ayrilmamis kurtlardir. 80,000'in üzerinde bilinen türden çogunlugu, özellikle de zararlilar olarak siniflandirilanlar tarimsal olarak önemlidir. Bu türlerden biri, genis bir bitki, çali ve ekin grubuna saldiran kök bogum nematodudur. Nematodes are known to be found in almost all kinds of environments (land, freshwater, sea). are worms that are not divided into microscopic segments. Over 80,000 known species the majority, especially those classified as pests, are agriculturally important. This One species is a root knot nematode that attacks a large group of plants, shrubs and crops.

Bu toprak kaynakli nematodlar yeni olusan köklere saldirir ve bodur gelisime, sismeye veya tümör olusumuna yol açar. Kökler daha sonra yarilabilir ve kökler bakteriler veya mantarlar gibi baska mikroorganizmalarin etkisine açik hale gelir. Azaltilmis toprak isleme veya toprak isleme olmadan çevre dostu uygulamalarla ve transjenik tohuma direnç kazanan çesitli nematod türleriyle, nematodla iliskili ürün kayiplarinin yükseliste oldugu görülmektedir. These soil-borne nematodes attack newly formed roots and cause stunted growth, swelling. or cause tumor formation. The roots can then be split and the roots become bacteria or become susceptible to the influence of other microorganisms such as fungi. reduced soil with environmentally friendly practices and transgenic seed without tillage or tillage The rise of nematode-related crop losses with various nematode species that become resistant. appears to be.

Toprak fumigantlari veya non-fumigantlari gibi kimyasal nematisitler, nematod infestasyonlari ile mücadelede uzun yillardir kullanimdadir. Bu nematisitler, ekimden önce topraga sentetik kimyasallarin tekrarli uygulamalarini gerektirebilir. Bunlarin toksisitesinden dolayi, kimyasal nematisitler, Çevre Koruma Örgütünün (EPA) detayli arastirmasina tabi tutulmus ve bazi durumlarda bunlarin kullanimi EPA tarafindan sinirlanmis veya kisitlanmistir. Metil-bromür ve organofosfatlar gibi geleneksel kimyasal nematisitlerin kullanimi asamali olarak azaltilmaya devam ettiginden, alternatif tedavi seçeneklerinin gelistirilmesine ihtiyaç dogmustur. Chemical nematicides such as soil fumigants or non-fumigants, nematode It has been in use for many years in the fight against infestations. These nematicides are may require repeated applications of synthetic chemicals to the soil first. These Due to their toxicity, chemical nematicides are recommended by the Environmental Protection Organization (EPA). have been investigated and in some cases their use has been regulated by the EPA. restricted or restricted. conventional, such as methyl-bromide and organophosphates. As the use of chemical nematicides continues to be gradually reduced, There is a need to develop alternative treatment options.

Bu ihtiyaci karsilamaya yönelik bir girisim, bakteriler, mantarlar, yararli nematodlar ve virüsler gibi biyolojik kontrol ajanlarinin kullanimidir. Bununla birlikte, simdiye kadar ticari açidan bu çabalar büyük ölçüde etkisiz olmustur. Dolayisiyla, nematod infestasyonuna açik olan tarimsal alanlarda bitki canliligini ve verimini arttirmak akimindan bütünüyle biyolojik tedavilerin genel etkinligi ile ilgili spekülasyon mevcuttur. An attempt to meet this need is an attempt to contain bacteria, fungi, beneficial nematodes and use of biological control agents such as viruses. However, so far Commercially, these efforts have been largely ineffective. Therefore, the nematode To increase plant vitality and yield in agricultural areas open to infestation There is currently speculation regarding the overall efficacy of purely biologic treatments.

Biyolojik kontrol ajanlarinin efikasitesini iyilestirmeye yönelik bir girisim WO sayili dokümanda ifsa edilen kompozisyonlar ve yöntemler, zararlilara ve patojenlere karsi bitki korumayi arttirmak için bir girisimde en az bir biyolojik kontrol ajani ve en az bir nematosidin, örnegin avermestinin kombinasyonlarina dayanmaktadir. Bir biyolojik nematisidin etki modu, bir kimyasal nematisidinkinden farkli olabildiginden, böyle bir kombinasyon tedavinin toplam efikasitesini iyilestirebilir fakat kimyasal nematisid bileseninden daha fazla toksisite saglayamaz. Dolayisiyla, çevre dostu biyolojik bilesenlerden yararlanmanin disinda, avermestin gibi bir miktar daha toksik geleneksel kimyasal nematisid kullanilmadan ayni zamanda bunlari iyilestirilmis bitki canliligi ve verimi saglayabilecekleri sekilde kullanan etkili kompozisyonlara ve yöntemlere ihtiyaç bulunmaktadir. An attempt to improve the efficacy of biological control agents WO compositions and methods disclosed in at least one biological control agent and at least one in an attempt to increase plant protection against It is based on combinations of a nematocide, eg avermestin. a biological Since the mode of action of a nematicide may differ from that of a chemical nematicide, such a The combination may improve the overall efficacy of therapy, but the chemical nematicide cannot provide more toxicity than its component. Therefore, environmentally friendly biological Apart from making use of the ingredients, some more toxic conventional ingredients such as avermestin without the use of chemical nematicides as well as improved plant vitality and There is a need for effective compositions and methods that use them in such a way that they can achieve efficiency. are available.

Parazitik nematodlarin yol açtigi giderek artan 'ürün kayiplari ile birlikte, alternatif olarak patojenik mantar hastaliklarina atfedilebilen bu türden birçok kayip bulunmaktadir. With increasing 'product losses' caused by parasitic nematodes, alternatively There are many such losses attributable to pathogenic fungal diseases.

Mevcut kimyasal maddelerdeki modifikasyonlara ve yeni etkili bilesikler ya da kimyasal madde kombinasyonlarinin gelistirilmesine ek olarak, biyolojik fungisitlerin gelistirilmesi ve kullanimi arastirilmaktadir. Modifications to existing chemicals and new effective compounds or chemical In addition to the development of agent combinations, biological fungicides development and use is under investigation.

Nematisidal bakteriler tek basina ürünler kadar her zaman tamamen etkili olmadigindan, fungisidal bakteriler, geleneksel kimyasal maddelerle bir replasman olmaktan ziyade bir tamamlayici olarak daha iyi islev görme egilimindedir. 5,215,747 sayili US patenti (WO 93/15611tin US karsiligi), fitopatenojenik fungal türlerden genel korumayi arttirmak için Bacillus subtilis (biyolojik birfungisit) ve kimyasal fungisitlerden olusan kompozisyonlari tarif etmektedir. fungisitler ve B. Subtilis suslarini (D747) içeren kompozisyonlar ifsa etmektedir. WO biyokontrol ajani kombinasyonunu ifsa etmektedir. US 6,406,690 sayili dokuman, Bacillus firmus CNCM 1-1582 sporlarinin nematosidal aktiviteye sahip olduguna iliskin bilgi vermektedir (tüm dokümanlar). Nematicidal bacteria are always fully effective as stand-alone products fungicidal bacteria are not a replacement with conventional chemicals. tends to function better as a complement rather than 5,215,747 US Patent No. (US equivalent of WO 93/15611), general use of phytopathogenic fungal species Bacillus subtilis (a biological fungicide) and chemical fungicides to increase protection describes the compositions. fungicides and compositions containing B. subtilis strains (D747). WO discloses the combination of biocontrol agents. US 6,406,690 document, It has been determined that Bacillus firmus CNCM 1-1582 spores have nematocidal activity. provides information (all documents).

Bitki parazitik nematodlarinin varliginda ve/veya patojenik mantar türlerinin kolaylastirdigi hastalik baskisi kosullarinda, en az bir biyolojik kontrol ajani ve en az bir böcek kontrol ajaninin tarimsal olarak etkili miktarlarini kombine ederek genel bitki canliligi ve verimini iyilestiren kompozisyonlar tarif edilmektedir. Biyolojik kontrol ajani, tarimsal faydasi ortaya konulmus olan ve ideal olarak bitkinin kök sistemine kolonize olabilen en az bir spor olusturan bakteri olabilir. Böcek kontrol ajani, kanitlanmis dogrudan nematisidal ya da fungisidal aktiviteye sahip olsun veya olmasin, uygulandigi bitkinin kök sistemi kütlesini artirma yetenegi kanitlanmis olan en az bir kimyasal insektisit olabilir. Mevcut bulusun kompozisyonlari tarimsal olarak kabul edilebilir bir raf ömrüne sahip olan tek bir stabil kompozisyon halinde formüle edilme veya kullanim zamaninda kombine edilme avantajina sahiptir (örnegin tank-karisimi). In the presence of plant parasitic nematodes and/or pathogenic fungal species under conditions of disease suppression that it facilitates, at least one biological control agent and at least one overall crop by combining agriculturally effective amounts of an insect control agent Compositions that improve viability and yield are described. biological control agent, colonize the root system of the plant, which has demonstrated agricultural utility and ideally There may be at least one spore-forming bacterium that can be Insect control agent, proven whether or not it has direct nematicidal or fungicidal activity, at least one plant with proven ability to increase root system mass of the plant to which it is applied Could be a chemical insecticide. Compositions of the present invention are considered agricultural Formulated as a single stable composition with a reasonable shelf life or it has the advantage of being combined at the time of use (eg tank-mix).

Bu bulusa göre kompozisyonlar istemler tarafindan karakterize edilmektedir. Compositions according to the present invention are characterized by the claims.

Burada tarif edilen kompozisyonlar, biyolojik kontrol ajani ve böcek kontrol ajanlari veya fungisitler, izoflabonlar ya da burada tarif edilen toprak asilama malzemeleri arasindaki sinerjik etkiden dolayi bitkilerin, bitki kisimlarinin veya bitki çogalma materyalinin tedavisinde sasirtici derecede yüksek insektisidal, nematisidal, akarisidal ya da fungisidal aktivite sergilemektedir. Compositions, biological control agent and insect control agents described herein or fungicides, isoflabons, or soil dressing materials described herein plants, plant parts or plant reproduction due to the synergistic effect between surprisingly high insecticidal, nematicidal, acaricidal or exhibits fungicidal activity.

Bir tohum ve/veya bitinin tedavi yöntemleri de saglanmaktadir. Söz konusu yöntem (a) en az bir biyolojik kontrol ajani ve en az bir böcek kontrol ajanindan etkili bir miktar içeren bir kompozisyon saglanmasi ve (b) kompozisyonun bitkiye uygulanmasi adimlarini içermektedir. Mevcut kompozisyonlar istenen herhangi bir biçimde, örnegin bir tohum kaplama, toprak sulama seklinde ve/veya dogrudan karik içerisine ve/veya yapraga püskürtme olarak uygulanabilir ve emerjanstan önce, emerjanstan sonra veya her iki sekilde uygulanabilir. Diger bir deyisle, kompozisyon tohuma, bitkiye ya da bitkinin meyvesine ya da bitkinin büyüdügü veya büyütülmek istendigi topraga uygulanabilir. Methods of treating a seed and/or louse are also provided. The method in question (a) at least one biological control agent and an effective amount of at least one insect control agent providing a composition containing, and (b) applying the composition to the plant. contains the steps. The present compositions may be used in any desired form, eg. as a seed coat, soil irrigation and/or directly into the furrow and/or It can be applied as a spray on the foliage and can be applied before, after or after emergence. can be applied both ways. In other words, the composition depends on the seed, plant or to the fruit of the plant or to the soil where the plant grows or is intended to be grown. applicable.

Yukaridaki ve diger yönler, detayli tarifnamede ve asagida izah edilen örneklerde ayrintili olarak açiklanmaktadir. The foregoing and other aspects are set out in the detailed description and in the examples explained below. are explained in detail.

Burada tarif edilen kompozisyonlarin, nematod istilasina ve fungal istilaya ugramis olan ortamlarda, biyolojik kontrol ajani veya böcek kontrol ajaninin tek basina uygulanmasi durumunda beklenecek olandan daha büyük derecede bitki canliligi ve verimi sagladigi saptanmistir. Burada tarif edilen böcek kontrol ajanlarindan en azindan bir kisminin, böcek sikintisi olmadiginda artan kök kütlesi sagladigi gösterilmistir, bu artan kök kütlesi rizosfer içerisinde yararli bakterilerin daha iyi yerlesmesine yol açar ve bu da ürün canliliginda ve verimdeki bitki parazitik nematodlarindan ya da mantarlardan kaynaklanan genel kayiplari azaltir. Bitkileri ve bitki materyalini tedavi ederken bu bilesenlerin fiziksel kombinasyonu ile birlikte, tercih edilen bir uygulamada, burada tarif edilen kompozisyonlar, spor olusturan, kökü kolonize eden bakteriler gibi canli biyolojik kontrol ajanlari için stabil bir ortam saglayacak sekilde formüle edilmistir. Ticari olarak uygun bir 'ürün üretmek için gerekli fiziksel ve kimyasal stabiliteye sahip olan son formülasyon için istenen özelliklere bagli olarak bulus niteligindeki her kompozisyon çesitli katki maddeleri eklenebilir. The compositions described herein are susceptible to nematode infestation and fungal infestation. Biological control agent or insect control agent alone greater degree of plant vitality and It has been found to provide efficiency. Most of the insect control agents described herein at least some of it provides increased root mass in the absence of insect infestation. It has been shown that this increased root mass allows better bacteria in the rhizosphere. This causes plant parasitic disease in crop viability and yield. reduces overall losses from nematodes or fungi. plants and combined with the physical combination of these components, the preferred In one embodiment, the compositions described herein are spore-forming, root a stable environment for living biological control agents such as colonizing bacteria It is formulated to provide required to produce a commercially viable 'product' depending on the desired properties for the final formulation with physical and chemical stability. As an inventive composition, various additives can be added.

Burada tarif edilen kompozisyonlar tercih edilen sekliyle en az bir biyolojik kontrol ajani kapsamaktadir. Bir biyolojik kontrol ajani, kanitlanmis tarimsal faydasi olan en az bir spor olusturan bakteriyi göstermektedir. Tercih edilen sekliyle, en az bir spor olusturan bakteri kök kolonize eden bakteridir (örnegin rizobakteri). Tarimsal fayda, bakterinin, bitkiye phylum Nematoda ya da Aschelminthes gibi bitki patenojenik mantarlar ve/veya toprak kökenli hayvanlarin zararli etkilerinden koruma saglama yetenegini göstermektedir. Bitki parazitik nematodlari ve mantarlara karsi koruma, bu toprak kökenli hayvanlar velveya zararli mikrobiyal populasyonlara zarar veren kitinolitik, proteolitik, kollajenolitik veya baska aktiviteler vasitasiyla meydana gelebilir. Ilave koruma örnegin bitki zararlilarina akut derecede toksik kimyasallarin üretimi gibi dogrudan ya da bitkinin, bitki patojenlerinin neden oldugu hasardan kendisini korumasina olan veren sistemik bir bitki tepkisinin ind'üklenmesi gibi dolayli koruma olabilir. Bu nematisidal ve fungisidal özellikleri sergileyen uygun bakteriler Grup B Grup B: Tek basina veya kombinasyon halinde Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus amyloliquefaciens, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus firmus, Bacillus kurstaki, Bacillus lentimorbus, Bacillus Iicheniformis, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nigrificans, Bacillus popillae, Bacillus pumilus, Bacillus siamensis, Bacillus sphaericus, Bacillus spp., Bacillus subtilis, Bacillus thuringiensis, Bacillus uniflagellatus, arti "Bergey's Manual of Systematic Bacteriology, First Ed. (1986)" yayininda Bacillus Cinsleri kategorisinde listelenenler. The compositions described herein preferably contain at least one biological control agent. covers. A biological control agent has at least one proven agricultural benefit. indicates spore-forming bacteria. Preferably, at least one spore-forming bacterium is root-colonizing bacterium (for example, rhizobacteria). agricultural benefit, bacterium, plant pathogenic fungi such as the phylum Nematoda or Aschelminthes and/or ability to provide protection from the harmful effects of soil-borne animals. shows. Protection against plant parasitic nematodes and fungi, this soil chitinolytic, which harms animals of origin and harmful microbial populations, may occur through proteolytic, collagenolytic or other activities. Additional protection, such as the production of chemicals that are acutely toxic to plant pests. directly or the plant itself from damage caused by plant pathogens. indirect protection, such as induction of a systemic plant response to it could be. Suitable bacteria are Group B exhibiting these nematicidal and fungicidal properties. Group B: Bacillus agri, Bacillus aizawai, Bacillus alone or in combination albolactis, Bacillus amyloliquefaciens, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus firmus, Bacillus kurtaki, Bacillus lentimorbus, Bacillus Iicheniformis, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nigrificans, Bacillus popillae, Bacillus pumilus, Bacillus siamensis, Bacillus sphaericus, Bacillus spp., Bacillus subtilis, Bacillus thuringiensis, Bacillus uniflagellatus, plus "Bergey's Manual of Systematic Bacteriology, First Ed. (1986)" are listed in the Bacillus Genus category.

Alternatif olarak Grup B ayrica Bacillus cereus'u içermektedir. Alternatively, Group B also includes Bacillus cereus.

Ozellikle tercih edilen bir uygulamada, nematisidal biyolojik kontrol ajani, 6,406,690 sayili U.S. Patentinde ifsa edildigi gibi en az bir B. firmus CNCM 1-1582 sporu ve/veya B. Cereus susu CNCM 1-1562 sporudur. Tercih edilen baska uygulamalarda, tarimsal olarak yararli bakteriler en az bir B. amyloliquefaciens IN937a, en az bir Bacillus subtilis susu GBOS veya en az bir B. pumilus susu GBS4itür. Yukarida listelenen bakterilerin dört türünün ve ayni zamanda tarimsal olarak yararli özellikler sergiledigi bilinen spor olusturan, kökü kolonize eden bakterilerin kombinasyonlari burada tarif edilmektedir. In a particularly preferred embodiment, the nematicidal biological control agent 6,406,690 U.S. no. At least one B. firmus CNCM 1-1582 spore and/or B. Cereus strain is a spore of CNCM 1-1562. In other preferred applications, agricultural as beneficial bacteria at least one B. amyloliquefaciens IN937a, at least one Bacillus subtilis strain GBOS or at least one B. pumilus strain GBS4. listed above four species of bacteria and also exhibit agriculturally beneficial properties. combinations of known spore-forming, root-colonizing bacteria are described here. is being done.

Burada tarif edilen özellikle tercih edilen uygulamalar ayni zamanda B. firmus CNCM kompozisyonlardir. Çok özel olarak bir nematisidal, insektisidal veya bitki büyümesini destekleyen aktiviteye sahip olan mutantlardir. En özel olarak tercih edilenler bir nematisidal aktiviteye sahip olan mutantlardir. Particularly preferred embodiments described herein are also known as B. firmus CNCM. are compositions. Very specifically a nematicidal, insecticidal or plant growth mutants with supporting activity. The most preferred are a mutants with nematicidal activity.

Kompozisyonlarda kullanilan en az bir biyolojik kontrol ajaninin miktari, son formülasyona ve ayni zamanda yararlanilan bitki ya da tohumun büyüklügü veya türüne bagli olarak degisiklik gösterebilir. Tercih edilen sekliyle, kompozisyonlardaki en az bir biyolojik kontrol ajani, tüm formülasyonun agirlik/agirlik olarak yaklasik % 2isi ila yaklasik % 80'idir. Daha tercih edilen sekliyle, kompozisyonlarda kullanilan en az bir biyolojik kontrol ajani tüm formülasyonun agirlik/agirlik olarak yaklasik % 5'i ila yaklasik % 65ii ve en çok tercih edilen sekliyle agirlik/agirlik olarak yaklasik % 10iu ila yaklasik % 607idir. The amount of at least one biological control agent used in the compositions formulation, as well as the size or size of the plant or seed used. may differ depending on the type. In its preferred form, in compositions at least one biological control agent, approximately 2% w/w of the entire formulation to about 80%. More preferably, at least the minimum used in the compositions. a biological control agent from about 5% w/w of the entire formulation to about 65% by weight and most preferably about 10% by weight/wt. is about 607%.

Burada tarif edilen kompozisyonlar ayrica en az bir böcek kontrol ajani içermektedir. The compositions described herein also contain at least one insect control agent.

Tercih edilen bir uygulamada, böcek kontrol ajani, insektisidal aktiviteye sahip olan fakat dogrudan nematisidal ya da fungisidal aktiviteye sahip olmayan ve kullanilan biyolojik kontrol ajanina karsi zararli aktiviteye sahip olmayan ve tercih edilen sekliyle ayni zamanda uygulama üzerine kök kütlesini arttirma yetenegine sahip olan herhangi bir insektisidal kimyasal bilesik ya da kompozisyon olabilir. Alternatif bir uygulamada, kompozisyonlar, nematisidal ya da fungisidal özellikler sergileyen en az bir ilave kimyasal bilesik içerebilir. Bu kompozisyonlar, son derece yüksek nematod infestasyon popülasyonlarina sahip olan cografi arazilerde kullanisli olabilir veya agir fungal hastalik baskisina karsi ilave fungisidal aktivite saglayabilir. Bitki veya bitki materyali nematisidal veya fungisidal kontrol ajaniyla ayri olarak veya es zamanli olarak tedavi edilebilir. In a preferred embodiment, the insect control agent has insecticidal activity. but without direct nematicidal or fungicidal activity and used having no deleterious activity against a biological control agent and preferably also any that has the ability to increase root mass upon application. an insecticidal chemical compound or composition. In an alternative application, The compositions contain at least one additive exhibiting nematicidal or fungicidal properties. May contain chemical compounds. These compositions are extremely high in nematode infestation. may be useful in geographic areas with populations of may provide additional fungicidal activity against disease pressure. plant or plant material separate or concurrent treatment with a nematicidal or fungicidal control agent can be done.

Uygun böcek kontrol ajanlari asagidaki (11) ila (122) gruplarinin bilesikleridir: Bu tarifnamede “yaygin adlariyla” belirtilen aktif bilesenler örnegin "The Pesticide Manual", 13th Ed., British Crop Protection Council 2003” yayinindan ve http://www.alanwood.net/pesticides web sayfasindan bilinmektedir. (11) Asetilkolinesteraz (AChE) inhibitörleri, Örnegin karbamatlar, örnegin alanikarb, aldikarb, aldoksikarb. alliksikarb. aminokarb, bendiokarb, benfurakarb, bufenkarb, butakarb, butokarboksim, bütoksikarboksim, karbaril, karbofuran, karbosulfan, kloetokarb, dimetilan, etiofenkarb, fenobukarb, fenotiokarb, formetanat, furatiokarb, izoprokarb, metam-sodyum, metiokarb, metomil, metolkarb, oksamil, pirimikarb, promekarb, propoksur, tiodikarb, tiofanoks, trimetakarb, XMC, ve ksililkarb; ya da organofosfatlar, örnegin asefat, azametifos, azinfos (-metil, -etil), bromofos-etil, bromfenvinfos (-metil), butatiofos, kadusafos, karbofenotion, kloretoksifos, klorfenvinfos, klormefos, klorpirifos (-metil/-etil), koumafos, siyanofenfos, siyanofos, klorfenvinfos, demeton-S-metil, demeton-S- metilsulfon, dialifos, diazinon, diklofention, diklorvos/DDVP, dikrotofos, dimetoat, dimetilvinfos, dioksabenzofos, disulfoton, EPN, etion, etoprofos, etrimfos, famphur, fenamifos, fenitrotion, fensulfotion, fention, flupirazofos, fonofos, formotion, fosmetilan, fostiazat, heptenofos, iodofenfos, iprobenfos, isazofos, isofenfos, izopropil, O-salisilat, izoksation, malation, mekarbam, metacrifos, metamidofos, metidation, mevinfos, monokrotofos, naled, ometoat, oksidemeton-metil, paration (-metil/-etil), fentoat, forat, fosalon, fosmet, fosfamidon, fosfokarb, foksim, pirimifos (-metiI/-etil), profenofos, propafos, propetamfos, protiofos, protoat, piraklofos, piridafention, piridation, kuinalfos, sebufos, sulfotep, suiprofos, tebupirimfos, temefos, terbufos, tetraklorvinfos, tiometon, triazofos, triklorfon, vamidotion, ve imisiyafos. (12) GABA geçitli klor'L'ir kanal antagonistleri, örnegin organoklorinler, örnegin kamfeklor, klordan, endosulfan, gamma-HCH, HCH, heptaklor, Iindan ve metoksiklor; ya da fiproller (fenilpirazoller), örnegin asetoprol, etiprol, fipronil, pirafluprol, piriprol ve vaniliprol. (13) Sodyum kanal modülatörleri/voltaja bagimli sodyum kanal blokerleri, örnegin piretroidler, örnegin akrinatrin, alletrin (d-cis-trans, d-trans), beta-ciflutrin, bifentrin, bioalletrin, bioalletrin S-siklopentil izomeri, bioetanometrini biopermetrin, bioresmetrin, klovaportrin, cis-sipermetrin, cis-resmetrin, cis-permetrin, klositrin, sikloprotrin, ciflutrin, Cihalotrin, cipermetrin (alfa-, beta-, teta-, zeta-), Cifenotrin, deltametrin, empentrin (1R izomeri), esfenvalerat, etofenproks, fenflutrin, fenpropatrin, fenpiritrin, fenvalerat, flubrocitirinat, flucitirinat, flufenproks, flumetrin, fluvalinat, fubfenproks, gamma- cihalotrin, imiprotrin, kadetrin, Iambda-cihalotrin, metoflutrin, permetrin (cis-, trans-), fenotrin (1 R trans izomeri), praletrin, proflutrin, protrifenbut, piresmetrin, resmetrin, RU 15525, silafluofen, tau-fluvalinat, teflutrin, teralletrin, tetrametrin (-1R-izomeri), tralometrin, transflutrin, ZXI 8901, piretrin (piretrum), eflusilanat; DDT; ya da metoksiklor. (14) Nikotinerjik asetilkolin reseptör agonistleri/antagonistleri, örnegin kloronikotiniller, örnegin asetamiprid, klotianidin, dinotefuran, imidakloprid, imidaklotiz, nitenpiram, nitiazin, tiakloprid, tiametoksam, D-1022, nikotin, bensultap, kartap, tiosultap-sodyum ve tiosilam. (15) Allosterik asetilkolin reseptör modülatörleri (agonistleri), örnegin spinosinler, örnegin spinosad ve spinetoram. (16) Klor'ür kanal aktivatörleri, örnegin mektinler/makrolitler, örnegin abamektin, emamektin benzoat, ivermektin, Iepimektin ve milbemektin; ya da (17) Jüvenil hormon analoglari, örnegin hidropren, kinopren, metopren, epofenonan, tripren, fenoksikarb, piriproksifen ve diofenolan. (18) Bilinmeyen ya da spesifik olmayan etki mekanizmalarina sahip olan aktif bilesenler, örnegin gazlama ajanlari, örnegin metil bromür, kloropikrin ve sülfüril flor'ur; selektif antifidanlar (antifeedants), örnegin kriyolit, pimetrozin, piriflukuinoazon ve flonisamid; ya da akar büyüme inhibitörleri, örnegin klofentezin, heksitiazoks, etoksazol. (19) Oksidatiffosforilasyon inhibitörleri, ATP bozucular, örnegin diafentiuron; organotin bilesikleri, örnegin azosiklotin, ciheksatin ve fenbutatin oksit; ya da propargit, tetradifon. (110) H proton gradiyentini keserek etki eden oksidatif fosforilasyon ayristiricilar, örnegin klorfenapir, binapakril, dinobuton, dinokap ve DNOC. (111) Böcek gut membraninin mikrobiyal bozuculari, Örnegin Bacillus thuringiensis suslari. (112) Kitin biyosentez inhibitörleri, örnegin benzoil'üreler, örnegin bistrifluron, klorfluazuron, diflubenzuron, flauzuron, flusikloksuron, flufenoksuron, heksaflumuron, lufenuron, novaluron, novi-flumuron, penfluron, teflubenzuron ya da triflumuron. (113) Buprofezin. (114) Deri degistirme bozucular, örnegin Ciromazin. (115) Ekdizon agonistleri/bozuculari, örnegin diaçilhidrazinler, örnegin kromafenozid, helofenozid, metoksifenozid, tebufenozid ve Fufenozid (JS118); ya da azadiraktin. (116) Oktopaminrjik agonistler, örnegin amitraz. (117) Alan III elektron tasima inhibitörleri/alan II elektron tasima inhibitörleri, örnegin hidrametilnon; acekuinosil; fluakripirim; ya da ciflumetofen ve siyenopirafen. (118) Elektron tasima inhibitörleri, örnegin METI akarisitler grubundan Alan l elektron tasima inhibitörleri, örnegin fenazakuin, fenpiroksimat, pirimidifen, piridaben, tebufenpirad, tolfenpirad ve rotenon; ya da voltaja bagimli sodyum kanal blokerler, örnegin indoksakarb ve metaflumizon. (119) Yag asidi biyosentez inhibitörleri, örnegin teronik asit türevleri, örnegin spirodiklofen ve spiromesifen; ya da tetramik asit türevleri, örnegin spirotetramat. (120) Bilinmeyen etki mekanizmasina sahip olan nöronal inhibitörler, örnegin bifenazat. (121) Riyanodin reseptör efektörleri, örnegin diamidler, örnegin flubendiamid, metilsulfoniletil)fitalamid, klorantraniliprol (Rynaxypyr), ya da Siyantraniliprol (Cyazypyr) (122) Bilinmeyen etki mekanizmasina sahip olan diger aktif bilesenler, örnegin amidoflumet, benklotiaz, benzoksimat, bromopropilat, buprofezin, kinometionat, klordimeform, klorobenzilat, klotiazoben, siklopren, dikofol, disiklanil, fenoksakrim, fentrifanil, flubenzimin, flufenerim, flutenzin, gossiplur, japoni'ure, metoksadiazon, petrol, potasyum oleat, piridalil, sulfluramid, tetrasul, triaraten ya da verbutin; ya da asagidaki bilinen aktif bilesikler: bilinmektedir), 4-{ [(6-fluorpirid- 3-il)metil](2,2-diflu0retil)amin0}furan-2(5H) -on (WO bilinmektedir), 4-{[(5,6-diklorpirid-3-il)metil](2-flu0retil)amin0}furan-2(5H) -on (WO klorpirid-S-il)metil](siklopropil)amino}furan-2(5H) -on (EP-A-O 539 588*den bilinmektedir), 4-{[(6-klorpirid-3-il)metil](metil)amin0}furan-2(5H) -on (EP-A-O 539 588tden bilinmektedir), [(6-klorpiridin-3- il)metil](metiI)oksido-)\4-sulfanilidensiyanamid diasteromerleri CH3 ÇH3 ya da [1-(6-triflu0rmetilpiridin-3-il)etil](metil)0ksid0- A4-sulfanilidensiyanamid (WO CH3 ÇH3 i \ /SICHa ` \ . /SICHS Tercih edilen bir uygulamada, böcek kontrol ajani asagidaki gruptan seçilir: Klotianidin, imidakloprid, tiakloprid, tiametoksam, asetamiprid, metiokarb, tiyokarb, beta-ciflutrin, ciflutrin, deltametrin, teflutrin, indoksakarb, spinosad, spinetoram, fipronil, etiprol, emamektin-benzoat, avermektin, spirodiklofen, spiromesifen, (triflorometil) etil] fenil}-N2-(1-metil-2-metilsulfoniletil)fitalamid, klorantraniliprol (Rynaxypyr) ya da Siyantraniliprol (Cyazypyr), sulfoksaflor, Tercih edilen baska bir uygulamada, böcek kontrol ajani transflutrindir. Suitable insect control agents are compounds of the following groups (11) to (122): Active ingredients referred to in this specification by their “common names” e.g. “The Pesticide” Manual”, 13th Ed., British Crop Protection Council 2003” and It is known from the web page http://www.alanwood.net/pesticides. (11) Acetylcholinesterase (AChE) inhibitors, eg carbamates such as alanicarb, aldicarb, aldoxycarb. allyxicarb. aminocarb, bendiocarb, benfuracarb, bufencarb, butacarb, butocarboxim, butoxycarboxim, carbaryl, carbofuran, carbosulfan, cloethocarb, dimethylan, etiophencarb, fenobucarb, phenotiocarb, formetanate, furatiocarb, isoprocarb, metam-sodium, methiocarb, metomyl, metolcarb, oxamil, pirimicarb, promecarb, propoxur, thiodicarb, thiophanox, trimethacarb, XMC, and xylylcarb; or organophosphates, eg acephate, azametifos, azinphos (-methyl, -ethyl), bromophos-ethyl, bromphenvinphos (-methyl), butatiofos, cadusafos, carbofenothion, chlorethoxyphos, chlorfenvinphos, chlormephos, chlorpyrifos (-methyl/-ethyl), koumaphos, cyanofenphos, cyanophos, chlorfenvinphos, demeton-S-methyl, demen-S- methylsulfone, dialifos, diazinon, diclofenthion, dichlorvos/DDVP, dicrotophos, dimethoate, dimethylvinphos, dioxabenzofos, disulfoton, EPN, ethion, etoprofos, etrimphos, famfur, fenamifos, fenitrothion, fensulfotion, fenthion, flupyrazofos, phonofos, formotion, fosmethylan, phostiazate, heptenofos, iodofenphos, iprobenfos, isazofos, isofenphos, isopropyl, O-salicylate, isoksation, malation, mecarbam, metacrifos, metamidofos, metidation, mevinphos, monocrothophos, naled, omethoate, oxidemeton-methyl, parathion (-methyl/-ethyl), phenthoate, forate, fosalon, fosmet, phosphamidone, phosphocarb, foxime, pyrimifos (-methyl/-ethyl), profenofos, propafos, propetamphos, protiophos, protoate, pyraclophos, piridafention, pyridation, quinalfos, sebufos, sulfotep, suiprofos, tebupyrimphos, temefos, terbufos, tetrachlorvinfos, thiomethone, triazophos, trichlorfon, vamidothion, and imisyaphos. (12) GABA-gated chloride channel antagonists, eg organochlorines, eg camphechlor, chlordane, endosulfan, gamma-HCH, HCH, heptachlor, Indane and methoxychlor; or fiprols (phenylpyrazoles), eg acetoprol, etiprole, fipronil, pirafluprol, pyriprol and vaniliprole (13) Sodium channel modulators/voltage dependent sodium channel blockers, eg pyrethroids, eg acrinatrin, allethrin (d-cis-trans, d-trans), beta-cifluthrin, bifenthrin, bioalletrin, bioalletrin S-cyclopentyl isomer, bioethanometrine biopermethrin, bioresmethrin, clovaportrin, cis-cypermethrin, cis-resmethrin, cis-permethrin, clocitrin, cycloprotrin, cifluthrin, Cihalothrin, cipermethrin (alpha-, beta-, theta-, zeta-), Cifenotrin, deltamethrin, empentrin (1R isomer), esfenvalerate, etofenprox, fenfluthrin, fenpropatrine, fenpyritrin, fenvalerate, flubrocitirinate, flucitirinate, flufenprox, flumethrin, fluvalinate, fubfenprox, gamma- cihalothrin, imiprothrin, cadethrin, Iambda-cihalothrin, methofluthrin, permethrin (cis-, trans-), phenotrin (1R trans isomer), pralethrin, profluthrin, protrifenbut, pyresmethrin, resmethrin, RU 15525, silafluofen, tau-fluvalinate, tefluthrin, terallethrin, tetramethrin (-1R-isomer), tralometrine, transfluthrin, ZXI 8901, pyrethrin (pyrethrum), eflusilanate; DDT; or methoxychlor. (14) Nicotinergic acetylcholine receptor agonists/antagonists, eg chloronicotinils, eg acetamiprid, clotianidine, dinotefuran, imidacloprid, imidaclotise, nitenpyram, nithiazine, thiacloprid, thiamethoxam, D-1022, nicotine, bensultap, cartap, thiosultap-sodium and thiosilam. (15) Allosteric acetylcholine receptor modulators (agonists), eg spinosyns, eg spinosad and spinetoram. (16) Chloride channel activators, eg mectins/macrolides, eg abamectin, emamectin benzoate, ivermectin, Iepimectin and milbemectin; or (17) Juvenile hormone analogues, eg hydroprene, quinoprene, methoprene, epofenan, triprene, fenoxycarb, pyriproxyfen and diophenolan. (18) Active substances with unknown or non-specific mechanisms of action components, for example gassing agents such as methyl bromide, chloropicrin and sulfuryl fluorine; selective antifeedants, such as cryolite, pymetrozine, pyrifluquinoazone and flonisamide; or mite growth inhibitors, eg clofentezine, hexthiazox, etoxazole. (19) Oxidativephosphorylation inhibitors, ATP disruptors, such as diafenthiuron; organotin compounds such as azocyclotin, cihexatin and fenbutatin oxide; or propargite, tetradiphone. (110) Oxidative phosphorylation disruptors that act by interrupting the H proton gradient, eg chlorfenapyr, binapacril, dinobuton, dinokap and DNOC. (111) Microbial disruptors of insect gut membrane, eg Bacillus thuringiensis shut up. (112) Chitin biosynthesis inhibitors, eg benzoylureas, eg bistrifluron, chlorfluazuron, diflubenzuron, flauzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, novi-flumuron, penfluron, teflubenzuron or triflumuron. (113) Buprofezin. (114) Skin change degraders, eg Ciromazine. (115) Ecdysone agonists/disruptors, eg diacylhydrazines, such as chromafenozide, helofenozide, methoxyfenozide, tebufenozide and Fufenozide (JS118); or azadirachtin. (116) Octopaminrgic agonists, eg amitraz. (117) Domain III electron transport inhibitors/domain II electron transport inhibitors, eg hydramethylnon; acequinosyl; fluacripim; or ciflumetophen and cyenopirafen. (118) Electron transport inhibitors, eg Alan 1 electron transport inhibitors from the group of METI acaricides, eg phenazaquin, fenpyroximate, pyrimidifen, pyridaben, tebufenpyrad, tolfenpyrad, and rotenone; or to voltage dependent sodium channel blockers, eg indoxacarb and metaflumizone. (119) Fatty acid biosynthesis inhibitors, eg teronic acid derivatives, eg spirodiclofen and spiromesifen; or tetramic acid derivatives such as spirotetramate. (120) Neuronal inhibitors of unknown mechanism of action, eg biphenazate (121) ryanodine receptor effectors, eg diamides, eg flubendiamide, methylsulfonylethyl)phthalamide, chlorantraniliprole (Rynaxypyr), or Cyantraniliprol (Cyazypyr) (122) Other active ingredients of unknown mechanism of action, e.g. amidoflumet, benclothiase, benzoxymate, bromopropylate, buprofezin, kinomethionate, chlordimeform, chlorobenzylate, clotiazoben, cycloprene, dicofol, dicyclanil, fenoxacrim, fentrifanil, flubenzimin, flufenerim, flutenzine, gossiplur, japoni'ure, methoxadiazon, petroleum, potassium oleate, pyridalyl, sulfluramide, tetrasul, triaraten or verbutin; or The following known active compounds are: known), 4-{ [(6-fluoropyrid-3-yl)methyl](2,2-difluoroethyl)amino}furan-2(5H)-one (WO known), 4-{[(5,6-dichloropyrid-3-yl)methyl](2-fluoethyl)amino}furan-2(5H)-one (WO chlorpyrid-S-yl)methyl](cyclopropyl)amino}furan-2(5H)-one (from EP-A-O 539 588) known), 4-{[(6-chloropyrid-3-yl)methyl](methyl)amino}furan-2(5H)-one (EP-A-O 539 588), [(6-chloropyridin-3-yl)methyl](methyl)oxido-)\4-sulfanilidenecyanamide diastereomers CH3 CH3 or [1-(6-trifluoromethylpyridin-3-yl)ethyl](methyl)OxideO-A4-sulfanilidenecyanamide (WO CH3 CH3 i \ /SICHa ` \ . /SICHS In a preferred embodiment, the insect control agent is selected from the following group: Clotianidine, imidacloprid, thiacloprid, thiamethoxam, acetamiprid, methiocarb, thiocarb, beta-cifluthrin, cifluthrin, deltamethrin, tefluthrin, indoxacarb, spinosad, spinetoram, fipronil, etiprole, emamectin-benzoate, avermectin, spirodiclofen, spiromesifen, (trifluoromethyl) ethyl]phenyl}-N2-(1-methyl-2-methylsulfonylethyl)phthalamide, chlorantraniliprole (Rynaxypyr) or Cyantraniliprol (Cyazypyr), sulfoxaflor, In another preferred embodiment, the insect control agent is transfluthrin.

Tercih edilen baska bir uygulamada, böcek kontrol ajani asagidaki gruptan seçilir: 4- dokümanindan bilinmektedir), 4-{[(6-fluorpirid-3-il)metil](2,2-difluoretil)amino}furan- dokümanindan bilinmektedir), 4-{[(5,6-diklorpirid-3-il)metil](2-fluoretil)amino}furan- bilinmektedir), 4-{[(6-klorpirid-3-il)metil](siklopropil)amin0}furan-2(5H) -on (EP-A-O 539 588 dokümanindan bilinmektedir), 4-{[(6-kl0rpirid-3-il)metil](metil)amino}furan-2(5H) - on (EP-A-O 539 588 dokümanindan bilinmektedir). Ozellikle tercih edilen böcek kontrol ajanlari klotianidin, imidakloprid, tiakloprid, tiametoksam ve asetamipriddir. Çok özel olarak tercih edilen bir böcek kontrol ajani imidaklopriddir. Çok özel olarak tercih edilen baska bir böcek kontrol ajani tiaklopriddir. Çok özel olarak tercih edilen baska bir böcek kontrol ajani tiametoksamdir. Çok özel olarak tercih edilen baska bir böcek kontrol ajani asetamipriddir. In another preferred embodiment, the insect control agent is selected from the following group: 4- known from documentation), 4-{[(6-fluorpyrid-3-yl)methyl](2,2-difluorethyl)amino}furan- known from documentation), 4-{[(5,6-dichloropyrid-3-yl)methyl](2-fluoretyl)amino}furan- known), 4-{[(6-chloropyrid-3-yl)methyl](cyclopropyl)amino}furan-2(5H)-one (EP-A-O 539 known from document 588), 4-{[(6-chloropyrid-3-yl)methyl](methyl)amino}furan-2(5H) - on (known from EP-A-O 539 588). Especially preferred insect control agents are clotianidine, imidacloprid, thiacloprid, thiamethoxam and acetamiprid. A very particularly preferred insect control agent is imidacloprid. Another particularly preferred insect control agent is thiacloprid. Another very particularly preferred insect control agent is thiamethoxam. Another particularly preferred insect control agent is acetamiprid.

En çok tercih edilen böcek kontrol ajani klotianidindir. The most preferred insect control agent is clothianidine.

Neonikotinoid bilesiklerin bitki büyümesini arttirma yetenegi, örnegin kök sistemi gelisimini arttirma yetenegi, bunlarin pestisidal aktivitesinden bagimsiz olarak ayrica 6,753,296 sayili US Patentinde açiklanmaktadir. The ability of neonicotinoid compounds to increase plant growth, e.g. root system The ability to enhance the development of these is also independent of their pesticidal activity. US Patent No. 6,753,296.

Alternatif bir uygulamada, bulus kompozisyonlari, yukarida listelenen nematidisidal olmayan böcek kontrol ajanlarindan en az iri ve biyolojik kontrol ajani ile kombinasyon halinde kullanilacak, dogrudan nematisidal aktivitesi olan istege bagli olarak ilave bir kimyasal bilesigi kapsamaktadir. Uygun nematisidal böcek kontrol ajanlari antibiyotik nematisitler, örnegin abamektin; karbamat nematisitler, örnegin benomil, karbofuran, karbosulfan ve kloetokarb; oksim karbamat nematisidler, örnegin alanikarb, aldikarb, aldoksikarb, oksamil; organofosforöz nematisidler, örnegin diamidafos, fenamifos, fostietan, fosfamidon, kadusafos, klorpirifos, diklofention, dimetoat, etoprofos, fensulfotion, fostiazat, heterofos, isamidofos, metoml, forat, fosfokarb, terbufos, tiyodikarb, tionazin, triazofos, imiciyafos ve mekarfonu kapsamaktadir. Diger uygun nematisidal böcek kontrol ajanlai asetoprol, benklotiaz, kloropikrin, dazomet, DBCP, DCIP, 1,2-dikl0ropr0pan, 1,3-dikloropropen, furfural, iyodometan, metam, metil bromür, metil izotiyosiyanat ve ksilenolleri kapsamaktadir. In an alternative embodiment, the compositions of the invention are the nematicidicidal compounds listed above. at least coarse of non-insect control agents and combination with biological control agent optionally an additional supplement with direct nematicidal activity to be used includes the chemical compound. Suitable nematicidal insect control agents antibiotics nematicides such as abamectin; carbamate nematicides, eg benomyl, carbofuran, carbosulfan and cloethocarb; oxime carbamate nematicides, eg alanicarb, aldicarb, aldoxycarb, oxamyl; organophosphorous nematicides, eg diamidafos, fenamiphos, phostiethane, fosfamidone, cadusafos, chlorpyrifos, diclofenthion, dimethoate, etoprofos, fensulfothion, phostiazate, heterophos, isamidofos, metoml, forate, phosphocarb, terbufos, includes thiodicarb, thionazine, triazophos, imiciafos and mecarphone. other suitable nematicidal insect control agents acetoprol, benclothiase, chloropicrin, dazomet, DBCP, DCIP, 1,2-dichloropropane, 1,3-dichloropropene, furfural, iodomethane, metam, methyl bromide, methyl isothiocyanate and xylenols.

Kompozisyonlarda kullanilan en az bir böcek kontrol ajaninin miktari, son formülasyona ve ayni zamanda tedavi edilecek bitki ve tohumun büyüklügüne bagli olarak degisiklik gösterebilir. Tercih edilen sekliyle, en az bir böcek kontrol ajani ya da fungisit, tüm formülasyona dayali olarak agirlik/agirlik olarak yaklasik % 1 ila yaklasik agirlik/agirlik olarak yaklasik % 5 ila yaklasik % 60 arasinda ve en çok tercih edilen sekliyle agirlik/agirlik olarak yaklasik % 10 ila yaklasik % 50 arasinda bulunur. The amount of at least one insect control agent used in the compositions depending on the formulation and also the size of the plant and seed to be treated. may vary. Preferably, at least one insect control agent or fungicide, from about 1% to about 1% w/w based on whole formulation from about 5% to about 60% w/w and most preferred in its form, it is found between about 10% and about 50% w/w.

Tipik olarak, biyolojik kontrol ajaninin bir böcek kontrol ajanina ya da bir fungisite olan 1:50 araligindadir. Bu oran araliklari, biyolojik kontrol ajaninin spor preparasyonunun 1011 /g içerdigi varsayimina dayanmaktadir. Spor preparasyonlarinin yogunluk olarak degismesi halinde, oranlarin, yukarida listelenen oran araliklari ile eslesecegi sekilde adapte edilmesi gerekir. 100:1 orani, böcek kontrol ajani ya da fungisitin 1 agirlik parçasina karsilik biyolojik kontrol ajaninin spor preparasyonlarinin 100 agirlik parçasi anlamina gelmektedir. Typically, the biological control agent is an insect control agent or a fungicide. It is in the range of 1:50. These ratio ranges are the same as that of the spore preparation of the biological control agent. It is based on the assumption that it contains 1011 /g. Concentration of sports preparations changes so that the rates match the ranges of rates listed above. must be adapted. 100:1 ratio, 1 weight of insect control agent or fungicide 100 weight parts of spore preparations of biological control agent versus part means.

Ayrica, bir ya da daha fazla fungisit içeren kompozisyonlar tarif edilmektedir. Bu fungisitler (F1) ila (F14) Iistelerinden seçilebilir: (F1) Nükleik asit sentez inhibitörleri, örnegin benalaksil, benalaksiI-M, bupirimat, klozilakon, dimetirimol, etirimol, furalaksil, himeksazol, metalaksil, metalaksil-M, ofuras, oksadiksil ve oksolinik asit. Also described are compositions comprising one or more fungicides. This fungicides can be selected from (F1) to (F14): (F1) Nucleic acid synthesis inhibitors, eg benalaxyl, benalaxyl-M, bupirimate, clozilacon, dimethirimol, ethrimol, furalaxyl, hymexazole, metalaxyl, metalaxyl-M, ofuras, oxadixyl and oxolinic acid.

(F2) Mitoz ve hücre bölünmesi inhibitörleri, örnegin benomil, karbendazim, klorfenazol, dietofenkarb, etaboksam, fuberidazol, pensikuron, tiabendazol, tiofanat, tiofanat-metil ve zoksamid. (F2) Mitosis and cell division inhibitors, eg benomyl, carbendazim, chlorphenazol, dietofencarb, ethaboxam, fuberidazole, pencicuron, thiabendazole, thiophanate, thiophanate-methyl and zoxamide.

(F3) Respirasyon inhibitörleri, örnegin Cl-respirasyon inhibitörü olarak diflumetorim; biksafen, boskalid, karboksin, fenfuram, flutolanil, fluopiram, furametpir, furmesikloks, izopirazam (QR-bileseni), izopirazam (98-bileseni), mepronil, oksikarboksin, pentiopirad, CIl-respirasyon inhibitörü olarak tifluzamid; amisulbrom, azoksistrobin, ciyazofamid, dimoksistrobin, enestroburin, famoksadon, fenamidon, fluoksastrobin, kresoksim-metil, metominostrobin, orisastrobin, pikoksistrobin, piraklostrobin, piribenkarb, CllI-respirasyon inhibitörü olarak trifloksistrobin. (F3) Respiratory inhibitors, eg diflumetorim as a Cl-respiration inhibitor; bicsfen, boskalid, carboxin, fenfuram, flutolanil, fluopiram, furametpir, furmecyclox, isopirazam (QR-component), isopirazam (98-component), mepronil, oxycarboxin, pentiopyrad, thifluzamide as a Cl1-respiration inhibitor; amisulbromine, azoxystrobin, ciyazofamide, dimoxystrobin, enestroburin, famoxadone, fenamidone, fluoxastrobin, kresoxim-methyl, metominostrobin, orisastrobin, picoxystrobin, pyraclostrobin, piribencarb, trifloxystrobin as a ClI-respiration inhibitor.

(F4) Ayirici olarak islev görebilen bilesikler örnegin binapakril, dinokap, fluazinam ve meptildinokap. (F4) Compounds that can function as separators such as binapacril, dinokap, fluazinam and meptildinocap.

(F5) ATP üretimi inhibitörleri, örnegin fentin asetat, fentin klorür, fentin hidroksit ve siltiofam. (F5) inhibitors of ATP production, eg fentin acetate, fentin chloride, fentin hydroxide and siltiofam

(F6) Amino asit ve/veya protein biyosentez inhibitörleri, örnegin andoprim, blastisidin- S, siprodinil, kasugamisin, kasugamisin hidroklorür hidrat, mepaniprim ve pirimetanil. (F6) Amino acid and/or protein biosynthesis inhibitors, eg andoprim, blasticidin- S, cyprodinil, kasugamycin, kasugamycin hydrochloride hydrate, mepaniprim, and pyrimethanil.

(F7) Sinyal transdüksiyon inhibitörleri, örnegin fenpiklonil, fludioksonil ve kuinoksifen. (F7) Signal transduction inhibitors such as fenpiclonil, fludioxonil and quinoxifen.

(F8) Lipid ve membran sentezi inhibitörleri, örnegin bifenil, klozolinat, edifenfos, etridiazol, iyodokarb, iprobenfos, iprodion, izoprotiolan, prosimidon, propamokarb, propamokarb hidroklorür, pirazofos, tolklofos-metil ve vinklozin. (F8) Lipid and membrane synthesis inhibitors, eg biphenyl, clozolinate, edifenphos, etridiazole, iodocarb, iprobenphos, iprodione, isoprothiolan, prosimidone, propamocarb, propamocarb hydrochloride, pyrazofos, tolclofos-methyl and vinclozine.

(F9) Ergosterol biyosentez inhibitörleri, örnegin aldimorf, azakonazol, bitertanol, bromukonazol, siprokonazol, diklobutrazol, difenokonazol, dinikonazol, dinikonazol-M, dodemorf, dodemorf asetat, epoksikonazol, etakonazol, fenarimol, fenbukonazol, fenheksamid, fenpropidin, fenpropimorf, flukuinkonazol, flurprimidol, flusilazol, flutriafol, furkonazol, furkonazol-cis, heksakonazol, imazalil, imazalil sülfat, imibenkonazol, ipkonazol, metkonazol, miklobutanil, naftifin, nuarimol, okspokonazol, paklobutrazol, pefurazoat, penkonazol, piperalin, prokloraz, propikonazol, protiokonazol, piributikarb, pirifenoks, kuinkonazol, simekonazol, spiroksamin, tebukonazol, terbinafin, tetrakonazol, triadimefon, triadimenol, tridemorf, triflumizol, triforin, tritikonazol, unikonazol, vinikonazol ve vorikonazol. (F9) Ergosterol biosynthesis inhibitors, eg aldimorph, azaconazole, bittanol, bromuconazole, ciproconazole, diclobutrazol, difenoconazole, diniconazole, diniconazole-M, dodemorph, dodemorph acetate, epoxiconazole, etaconazole, phenarimol, fenbuconazole, fenhexamide, fenpropidine, fenpropimorph, fluquinconazole, flurprimidol, flusilazole, flutriafol, furconazole, furconazole-cis, hexaconazole, imazalil, imazalil sulfate, imibenconazole, ipconazole, metconazole, miclobutanil, naftifine, nuarimol, oxpoconazole, paclobutrazol, pefurazoate, penconazole, piperaline, prochlorase, propiconazole, protioconazole, piributicarb, pirifenox, quinconazole, cimeconazole, spiroxamine, tebuconazole, terbinafine, tetraconazole, triadimefon, triadimenol, tridemorph, triflumizole, triforin, triticonazole, uniconazole, viniconazole and voriconazole.

(F10) Hücre duvari sentez inhibitörleri, örnegin bentiavalikarb, dimetomorf, flumorf, iprovalikarb, mandipropamid, polioksinler, polioksorim, protiyokarb, validamisin A ve valifenal. (F10) Cell wall synthesis inhibitors, eg bentiavalicarb, dimethomorph, flumorph, iprovalicarb, mandipropamide, polyoxins, polyoxorim, prothiocarb, validamycin A and validenal

(F11) Melanin biyosentez inhibitörleri, örnegin karpropamid, diklosimet, fenoksanil, fitalid, pirokuilon ve trisiklazol. (F11) Melanin biosynthesis inhibitors, eg carpropamide, diclosimet, fenoxanil, phthalide, pyroquilon and tricyclazole.

(F12) Bir konagin savunmasinin indükleyebilen bilesikler, örnegin acibenzolar-S-metil, probenazol ve tiadinil. (F12) Compounds capable of inducing a host's defenses, eg acibenzolar-S-methyl, probenazole and tiadinil.

(F13) Çok alanli bir etkiye sahip olabilen bilesikler, örnegin bordo karisimi, kaptafol, kaptan, klorotalonil, bakir naftenat, bakir oksit, bakir oksiklorür, bakir preparasyonlari, örnegin bakir hidroksit, bakir sülfat, diklofluanid, ditianon, dodin, dodin serbest baz, ferbam, florofolpet, folpet, guazatin, guazatin asetat, iminoktadin, iminoktadin albesilat, iminoktadin triasetat, manbakir, mancozeb, maneb, metiram, metiram çinko, oksin- bakir, propamidin, propineb, sülfür ve sülfür preparasyonlari, örnegin kalsiyum polisülfit, tiram, tolilfluanid, zineb ve ziram. (F13) Compounds that can have a multi-site effect, eg burgundy mix, captafol, captain, chlorothalonil, copper naphthenate, copper oxide, copper oxychloride, copper preparations, for example copper hydroxide, copper sulfate, diclofluanide, dithionone, dodine, dodine free base, ferbam, fluorofolpet, folpet, guazatin, guazatin acetate, iminoctadine, iminoctadine albesylate, iminoctadine triacetate, manbakir, mancozeb, maneb, metiram, metiram zinc, auxin- copper, propamidine, propineb, sulfur and sulfur preparations, eg calcium polysulfide, tiram, tolylfluanid, zineb and ziram.

(F14) baska bilesikler, örnegin 2,3-dibutyI-G-klorothien0[2,3-d]pirimidin-4(3H) -on, etil (22) -3-aminfenil]- (diflorometil) -1-metil-1H-pirazoI-4-Karboksamid, 3-(difl0rometil) -1-metiI-N-(3*,4',5'- metilfenoksi) -5-fl0ropirimidin-4-il]oksi}fenil) -2-(metoksiimino) -N-metiletanamid, (2E) - hidroksibenzamid, 5-metoksi-2-metil-4-(2-{[({(1 E) -1-[3-(trifl0rometil) (metoksiimino) -N-metiI-2-(2-{[({(1E) -1-[3-(trifl0r0metil)fenil]etiliden}amino)oksi] metil}fenil)etanamid, (2E) -2-(metoksiimino) -N-metil-2-{2-[(E) -({1-[3-(trifl0r0metil)fenil] fenilethenyl]0ksi}fenil)etiliden]min0}0ksi)metil]feniI}-2-(met0ksiimin0) -N- dimetiI-2,3 -dihidr0-1H-inden-1-il) -1Himidazol- 5 -karboksilat, N-etil-N-metiI-N'- {2- metil-5-(trifl0rometil) -4-[3-(trimetiIsiliI)pr0p0ksi]fenil}imidoformamid, N,-{5-(diflorometil) -2-metiI-4-[3-(trimetiIsilil)propoksi]feniI}-N-etil-N-metilimido-formamid, O-{1-[(4- metoksifenoksi)metiI]-2,2-dimetilpr0pil}1 H-imidazol-1 -ka rbotioate, N-[2-(4-{[3-(4- 1,3,4-thiadiazoI-2-tiol, propamokarb-fosetil, 1-[(4-metoksifenoksi)metil] -2,2- (triflorometil) -1H-pirazoI-4-karb0ksamid, 2,3,5,6-tetrakloro-4-(metiIsulfonil)piridin, 2- bütoksi-ö-iodo-ß-propiI-4H-kromen-4-0n, 2-fenilfenol ve tuzlari, 3-(diflorometil) -1- trikloropiridin-2,6-dikarb0nitril, 3-[5-(4-klorofenil) -2,3-dimetilisoxazolidin-S-il]piridin, 3- diflorofenil) -3,6-dimetilpirida2ine, kuinolin-8-ol, kuinolin-8-ol sülfat (2:1) (salt), 5-metil- dihidr0[1,2,4]triazol0[1,5-alpiriniidin-7-amin, bentiazol, betoksazin, kapsimisin, karvon, Chinometionat, kloroneb, cufraneb, ciyflufenamid, simoksanil, ciyprosulfamid, dazomet, debakarb, diklorofen, diklomezin, dikloran, difenzokuat, difenzokuat metilsülfat, difenilamin, ekomat, ferimzon, flumetover, fluopikolid, floroimid, flusulfamid, flutianil, fosetiI-alüminyum, fosetiI-kalsiyum, fosetiI-sodyum, heksaklorobenzen, irumamisin, izotianil, metasulfokarb, metil (2E) -2-{2-[({siklopr0pil[(4- metoksifeniI)imin0]metil}tio)meti|]fenil}-3-met0ksiakrilat, metil izotiyosiyanat, metrafenon, (5-bromo-2-metoksi-4-metilpiridin-3-il)(2,3,4-trimetoksi-6-metilfenil) - metanon, mildiomisin, tolnifanid, N-(4-klorobenzil) -3-[3-metoksi-4-(prop-2-in-1- iloksi)fenil]pr0panamid, N-[(4-kl0r0feniI)(siyano)metil]-3-[3-metoksi-4-(prop-2-in-1- iloksi)fenil]pr0panamid, N-[(5-br0mo-3-kloropiridin-2-il)metil]-2,4-dikloropiridin-S- karboksamid, N-[1-(5 -br0m0-3 -kloropiridin-2-il)etiI]-2,4-dikloropiridin-3-karboksamid, N-{(E) -[(sikl0pr0pilmetoksi)imin0][6-(diflorometoksi) -2,3-diflorofenil]metiI}-2-fenilaset- amid, natamisin, nikel dimetilditiokarbamat, nitrothaI-izopropil, oktilinon, oksamokarb, oksifenthiin, pentaklorofenol ve tuzlari, fenazin-1-karboksilik asit, fenotrin, fosforöz asit ve bunun tuzlari, propamokarb fosetilat, propanosin-sodyum, prokuinazid, pirrolnitrin, 1Hpirazol-1-karboti0ate, tekloftalam, teknazen, triazoksid, triklamid, 5-kloro-N'-fenil- Nr-prop-2-yn-1-iItiophene-2-sulfonohydrazide ve zarilamid. (F14) other compounds such as 2,3-dibutyI-G-chlorothienO[2,3-d]pyrimidin-4(3H)-one, ethyl (22) -3-aminephenyl]- (difluoromethyl)-1-methyl-1H-pyrazole-4-Carboxamide, 3-(difluoromethyl)-1-methyl-N-(3*,4',5'- methylphenoxy) -5-fluoropyrimidin-4-yl]oxy}phenyl) -2-(methoxyimino)-N-methylethanamide, (2E) - hydroxybenzamide, 5-methoxy-2-methyl-4-(2-{[({(1E)-1-[3-(trifluoromethyl)) (methoxyimino)-N-methyl-2-(2-{[({(1E) -1-[3-(trifluoromethyl)phenyl]ethylidene}amino)oxy] methyl}phenyl)ethanamide, (2E)-2-(methoxyimino)-N-methyl-2-{2-[(E) -({1-[3-(trifluoromethyl)phenyl] phenylthenyl]Oxy}phenyl)ethylidene]minO}Oxy)methyl]phenyl}-2-(methoxyimin0)-N- dimethyl-2,3-dihydro-1H-inden-1-yl) -1Himidazole-5-carboxylate, N-ethyl-N-methyl-N'- {2- methyl-5-(trifluoromethyl)-4-[3-(trimethylylsilyl)pr0p0xy]phenyl}imidoformamide, N,-{5-(difluoromethyl) -2-methyl-4-[3-(trimethylsilyl)propoxy]phenyl}-N-ethyl-N-methylimido-formamide, O-{1-[(4- methoxyphenoxy)methyl]-2,2-dimethylpropyl}1H-imidazole-1-carbotioate, N-[2-(4-{[3-(4-) 1,3,4-thiadiazoI-2-thiol, propamocarb-focetyl, 1-[(4-methoxyphenoxy)methyl]-2,2- (trifluoromethyl)-1H-pyrazole-4-carboxamide, 2,3,5,6-tetrachloro-4-(methylsulfonyl)pyridine, 2- butoxy-o-iodo-ß-propyl-4H-chromene-4-0n, 2-phenylphenol and its salts, 3-(difluoromethyl) -1- trichloropyridine-2,6-dicarboxytrile, 3-[5-(4-chlorophenyl)-2,3-dimethylisoxazolidin-S-yl]pyridine, 3- difluorophenyl)-3,6-dimethylpyrida2ine, quinolin-8-ol, quinolin-8-ol sulfate (2:1) (pure), 5-methyl- dihydro[1,2,4]triazolo[1,5-alpyriniidin-7-amine, benthiazole, betaxazine, capsimycin, carvone, Chinomethionate, chloroneb, cufraneb, ciyflufenamide, cymoxanil, cyprosulfamide, dazomet, debacarb, dichlorophene, diclomezin, dichloran, diphenzoquat, diphenzoquat methylsulfate, diphenylamine, ecomate, ferimzon, flumetover, fluopicolide, fluoroimide, flusulfamide, flutianyl, focetyl-aluminum, focetyl-calcium, fosethyl-sodium, hexachlorobenzene, irumamycin, isothianyl, metasulfocarb, methyl (2E) -2-{2-[({cyclopropyl[(4-) methoxyphenylimimin0]methyl}thio)methyl]phenyl}-3-methoxyacrylate, methyl isothiocyanate, metrafenone, (5-bromo-2-methoxy-4-methylpyridin-3-yl)(2,3,4-trimethoxy-6-methylphenyl) - methanone, mildiomycin, tolnifanide, N-(4-chlorobenzyl)-3-[3-methoxy-4-(prop-2-yn-1-) yloxy)phenyl]propanamide, N-[(4-chlorophenyl)(cyano)methyl]-3-[3-methoxy-4-(prop-2-yn-1-) yloxy)phenyl]propanamide, N-[(5-br0mo-3-chloropyridin-2-yl)methyl]-2,4-dichloropyridine-S- carboxamide, N-[1-(5-br0m0-3-chloropyridin-2-yl)ethyl]-2,4-dichloropyridine-3-carboxamide, N-{(E)-[(cyclopropylmethoxy)imin0][6-(difluoromethoxy)-2,3-difluorophenyl]methyl}-2-phenylacet- amide, natamycin, nickel dimethyldithiocarbamate, nitrothaI-isopropyl, octilinone, oxamocarb, oxyfenthiin, pentachlorophenol and its salts, phenazine-1-carboxylic acid, phenotrin, phosphorous acid and its salts, propamocarb fosethylate, propanosine-sodium, proquinazide, pyrrolnitrin, 1Hpyrazole-1-carbotylate, monophthalam, technazen, triazoxide, trichamide, 5-chloro-N'-phenyl- Nr-prop-2-yn-1-ylthiophene-2-sulfonohydrazide and zarilamide.

Alternatif olarak, burada tarif edilen kompozisyonlar bir biyolojik kontrol ajani ve (F1) ila (F14) gruplarindan seçilen en az bir fungisit içermektedir. Alternatively, the compositions described herein may contain a biological control agent and (F1). at least one fungicide selected from the groups to (F14).

Ayrica, burada tarif edilen kompozisyonlar bir biyolojik kontrol ajani, (11) ila (122) gruplarindan seçilen en az bir böcek kontrol ajani ve (F1) ila (F14) gruplarindan seçilen en az bir fungisit içermektedir. In addition, the compositions described herein are used as a biological control agent, (11) to (122) at least one insect control agent selected from groups (F1) to (F14) contains at least one fungicide.

Tercih edilen bir uygulamada, söz konusu fungisitler asagidaki listeden seçilir: 1H-pirazoI-4-karboksamid), Karbendazim, Karboksin, Fenamidon, Fludioksonil, Fluopikolid, Fluoksastrobin, Flukuinkonazol, Flutriafol, lpkonazol, Iprodion, Izotianil, Mefenoksam, Metalaksil, Pensikuron, Prokloraz, Protiokonazol, Piraklostrobin, Pirimetanil, Siltiofam, Tebukonazol, Thiram, Tolilfluanid, Triadimenol, Triazoksid, Trifloksistrobin, Triflumuron, Tritikonazol. In a preferred embodiment, the fungicides are selected from the following list: 1H-pyrazole-4-carboxamide), Carbendazim, Carboxin, Phenamidone, Fludioxonil, Fluopicolide, Fluoxastrobin, Fluquinconazole, Flutriafol, Ipconazole, Iprodion, Izotianil, Mefenoxam, Metalaxyl, Pencicuron, Prochloraz, Protioconazole, Pyraclostrobin, Pyrimethanil, Siltiofam, Tebuconazole, Thiram, Tolylfluanid, Triadimenol, Triazoxide, Trifloxystrobin, Triflumuron, Triticonazole.

Tercih edilen baska bir uygulamada, söz konusu fungisit N-{2-[1,1'-bi(sikl0pr0pil) -2- il]fenil}-3-(difl0rometil) -1-metiI-1H-pirazoI-4-karb0ksamiddir. In another preferred embodiment, said fungicide is N-{2-[1,1'-bi(cycl0pr0pil)-2- yl]phenyl}-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide.

Burada tarif edilen tercih edilen kompozisyonlar, Bacillus firmus susu CNCM 1-1582 sporu olan bir biyolojik kontrol ajani ve (F1) ila (F14) listesinden seçilen en az bir fungisit içermektedir. Preferred compositions described herein are Bacillus firmus strain CNCM 1-1582 a biological control agent with spores and at least one selected from the list (F1) to (F14) Contains fungicide.

Tercih edilen baska bir uygulamada, burada tarif edilen kompozisyonlar, Bacillus cereus susu CNCM 1-1562 sporu olan bir biyolojik kontrol ajani ve (F1) ila (F14) listesinden seçilen en az bir fungisit içermektedir. In another preferred embodiment, the compositions described herein contain Bacillus cereus strain CNCM 1-1562 spore, a biological control agent and (F1) to (F14) Contains at least one fungicide selected from the list.

Burada tarif edilen `Özellikle tercih edilen kompozisyonlar, Bacillus firmus susu CNCM 1-1582 sporu olan bir biyolojik kontrol ajani ve asagidaki listeden seçilen en az bir fungisit içermektedir: Azoksistrobin, Boskalid, BYF 14182, Karbendazim, Karboksin, Fenamidon, Fludioksonil, Fluopikolid, Fluoksastrobin, Flukuinkonazol, Flutriafol, lpkonazol, Iprodion, Izotianil, Mefenoksam, Metalaksil, Pensikuron, Prokloraz, Protiokonazol, Piraklostrobin, Pirimetanil, Siltiofam, Tebukonazol, Thiram, Tolilfluanid, Triadimenol, Triazoksid, Trifloksistrobin, Triflumuron, Tritikonazol. ` Particularly preferred compositions described herein are Bacillus firmus strain CNCM A biological control agent with 1-1582 spores and at least one selected from the list below Contains fungicides: Azoxystrobin, Boskalid, BYF 14182, Carbendazim, Carboxin, Phenamidone, Fludioxonil, Fluopicolide, Fluoxastrobin, Fluquinconazole, Flutriafol, Ipconazole, Iprodion, Isotianil, Mefenoxam, Metalaxyl, Pencicuron, Prochloraz, Protioconazole, Pyraclostrobin, Pyrimetanil, Siltiofam, Tebuconazole, Thiram, Tolilfluanid, Triadimenol, Triazoxide, Trifloxystrobin, Triflumuron, Triticonazole.

Burada tarif edilen ve bir biyolojik kontrol ajani ve en az bir böcek kontrol ajani içeren tercih edilen kombinasyonlar (C1) ya da (02) kombinasyonlari ya da (C1-9) ila (C1-10) kombinasyonlaridir: (C1) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin asagidaki listeden seçildigi kombinasyonlar: Klotianidin, imidakloprid, tiakloprid, tiametoksam, asetamiprid, metiokarb, tiodikarb, beta-ciflutrin, ciflutrin, deltametrin, teflutrin, indoksakarb, spinosad, spinetoram, fipronil, etiprol, emamektin-benzoat, avermektin, spirodiklofen, spiromesifen, spirotetramat, N2-(1-metiI-2-metilsulf0niletil)phthaI-amid, klorantraniliprol (Rynaxypyr), ya da Siyantraniliprol (Cyazypyr), sulfoksaflor, 4-{[(6-brompirid-3-il)metil](2- dokümanindan bilinmektedir), 4-{[(2-kl0r-1,3-tiazol-5-il)metil](2-fluoretil)amino}furan- dokümanindan bilinmektedir), 4-{[(6-klor-5-flu0rpirid-3-iI)metil](metil)amino}furan- bilinmektedir), 4-{[(6-klor-5- fluorpirid-S-il)metil](siklopropil)amino}furan-2(5H) -on (WO il)metil](siklopropil)amino}furan-2(5H) -on (EP-A-O 539 588 dokümanindan dokümanindan bilinmektedir), [(6-kl0rpiridin-3-il)metil](metil)0ksid0-)\4- dokümanindan bilinmektedir) ve bunun (A) ve (B) diastereomerleri. Containing a biological control agent and at least one insect control agent described herein preferred combinations (C1) or combinations (02), or (C1-9) to (C1-10) are combinations of: (C1) In which the biological control agent is Bacillus firmus CNCM 1-1582 spore and Combinations where the insect control agent is selected from the following list: Clotianidin, imidacloprid, thiacloprid, thiamethoxam, acetamiprid, methiocarb, thiodicarb, beta-cifluthrin, cifluthrin, deltamethrin, tefluthrin, indoxacarb, spinosad, spinetoram, fipronil, etiprole, emamectin-benzoate, avermectin, spirodiclofen, spiromesifen, spirotetramate, N2-(1-methyl-2-methylsulfonylethyl)phthalene-amide, chlorantraniliprole (Rynaxypyr), or Cyantraniliprole (Cyazypyr), sulfoxafluor, 4-{[(6-brompyrid-3-yl)methyl](2- known from documentation), 4-{[(2-chlor-1,3-thiazol-5-yl)methyl](2-fluoretyl)amino}furan- known from documentation), 4-{[(6-chloro-5-fluoropyrid-3-yl)methyl](methyl)amino}furan- known), 4-{[(6-chloro-5-fluorpyridine-S-yl)methyl](cyclopropyl)amino}furan-2(5H)-one (WO yl)methyl](cyclopropyl)amino}furan-2(5H)-one (from EP-A-O 539 588 known from documentation), [(6-chlorpyridin-3-yl)methyl](methyl)Oxide0-)\4- known from the document) and its diastereomers (A) and (B).

(CZ) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin asagidaki listeden seçildigi kombinasyonlar: Klotianidin, imidakloprid, tiakloprid, tiametoksam, asetamiprid, metiokarb, tiodikarb, beta-ciflutrin, ciflutrin, deltametrin, teflutrin, indoksakarb, spinosad, spinetoram, fipronil, etiprol, emamektin-benzoat, avermektin, spirodiklofen, spiromesifen, spirotetramat, flubendiamid, (R),(S) -3-klor0-N-[2- metiI-4-[1,2,2,2-tetrafloro-1-(triflorometil)etil]fenil}- N2-(1-metiI-2-metilsulfoniletil)fitalamid, klorantraniliprol (Rynaxypyr), ya da Siyantraniliprol (Cyazypyr), sulfoksaflor, 4-{[(6-brompirid-3-il)metil](2- dokümanindan bilinmektedir), 4-{[(2-kl0r-1,3-tiazol-5-il)metil](2-fluoretil)amino}furan- dokümanindan bilinmektedir), 4-{[(6-klor-5-fluorpirid-S-il)metil](metil)amino}furan- bilinmektedir), 4-{[(6-klor-5- fluorpirid-3-il)metil](siklopropil)amino}furan-2(5H) -on (WO il)metil](siklopropil)amino}furan-2(5H) -on (EP-A O 539 588 dokümanindan dokümanindan bilinmektedir), [(ö-klorpiridin-S-il)metil](metil)oksido-)\4- dokümanindan bilinmektedir) ve bunun (A) ve (B) diastereomerleri. (CZ) In which the biological control agent is Bacillus firmus CNCM 1-1582 spore and Combinations where the insect control agent is selected from the following list: Clotianidin, imidacloprid, thiacloprid, thiamethoxam, acetamiprid, methiocarb, thiodicarb, beta-cifluthrin, cifluthrin, deltamethrin, tefluthrin, indoxacarb, spinosad, spinetoram, fipronil, etiprole, emamectin-benzoate, avermectin, spirodiclofen, spiromesifen, spirotetramate, flubendiamide, (R),(S)-3-chloro-N-[2-methyl-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]phenyl}- N2-(1-methyl-2-methylsulfonylethyl)phthalamide, chlorantraniliprole (Rynaxypyr), or Cyantraniliprole (Cyazypyr), sulfoxafluor, 4-{[(6-brompyrid-3-yl)methyl](2- known from documentation), 4-{[(2-chlor-1,3-thiazol-5-yl)methyl](2-fluoretyl)amino}furan- known from documentation), 4-{[(6-chloro-5-fluoropyridine-S-yl)methyl](methyl)amino}furan- known), 4-{[(6-chloro-5-fluorpyrid-3-yl)methyl](cyclopropyl)amino}furan-2(5H)-one (WO yl)methyl](cyclopropyl)amino}furan-2(5H)-one (from EP-A 0 539 588 known from documentation), [(o-chloropyridin-S-yl)methyl](methyl)oxido-)\4- known from the document) and its diastereomers (A) and (B).

(C1-1) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin klotianidinden olustugu kombinasyonlar. (C1-1) In that the biological control agent is Bacillus firmus CNCM 1-1582 spore. and combinations of the insect control agent clotianidine.

(C1-2) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin imidaklopridden olustugu kombinasyonlar. (C1-2) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and combinations of the insect control agent imidacloprid.

(C1-3) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin klotianidin ve imidaklopridden olustugu kombinasyonlar. (C1-3) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and combinations of the insect control agent clothianidine and imidacloprid.

(C1-4) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin klotianidin ve ß-ciflutrinden olustugu kombinasyonlar. (C1-4) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and combinations of the insect control agent clothianidine and ß-cifluthrin.

(C1-5) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin tiametoksam ve teflutrinden olustugu kombinasyonlar. (C1-5) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and combinations of the insect control agent thiamethoxam and teflutrin.

(C1-6) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin klotianidin ve imidaklopridden olustugu kombinasyonlar. (C1-6) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and combinations of the insect control agent clothianidine and imidacloprid.

(C1-7) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin tiodikarb ve imidaklopridden ya da tiodikarb ve klotianidinden olustugu kombinasyonlar. (C1-7) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and insect control agent from thiodicarb and imidacloprid or from thiodicarb and clotianidine combinations formed.

(C1-8) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin Klotianidin ve fipronil ya da fipronil ve imidaklopridden olustugu kombinasyonlar. (C1-8) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and insect control agent from Clotianidin and fipronil or from fipronil and imidacloprid combinations formed.

(C1-9) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin asagidakilerden olustugu kombinasyonlar a) klorantaniliprol, ve b) imidakloprid ya da klotianidin ya da tiametoksam ya da tiakloprid ya da asetamiprid ya da nitenpiram veya sulfoksaflor ya da devam eden listeden seçilen bilesiklerden biri: 4-{ [(6-brompirid-3-il)metil](2-fluoretil)amino}furan-2(5H) -on (WO dokümanindan bilinmektedir), 4-{[(6-klorpirid-3-il)metil](2-fluoretil)amino}furan-2(5H) - dokümanindan bilinmektedir), 4-{[(5,6- diklorpirid-3-il)metil](2-fluoretil)amino}furan- bilinmektedir), 4-{[(6-klorpirid-3- il)metil](siklopropil)amino}furan-2(5H) -on (EP-A-O bilinmektedir), [1-(6-klorpiridin-3-il)etil](metil)oksido-A4-sulfanilidensiyanamid (WO (C1-10) Içerisinde biyolojik kontrol ajaninin Bacillus firmus CNCM 1-1582 sporu oldugu ve böcek kontrol ajaninin asagidakilerden olustugu kombinasyonlar a) siyantraniliprol b) imidakloprid ya da klotianidin ya da tiametoksam ya da tiakloprid ya da asetamiprid ya da nitenpiram veya sulfoksaflor ya da devam eden listeden seçilen bilesiklerden biri: 4-{ [(6-brompirid-3-il)metil](2-fluoretil)amino}furan-2(5H) -on (WO dokümanindan bilinmektedir), 4-{[(6-kl0rpirid-3-il)metil](2-flu0retil)amin0}furan-2(5H) - dokümanindan bilinmektedir), 4-{[(5,6- diklorpirid-3-il)metil](2-fluoretil)amino}furan- bilinmektedir), 4-{[(6-klorpirid-3- il)metil](siklopropil)amino}furan-2(5H) -on (EP-A-O 2(5H) -on (EP-A-O 539 588 dokümanindan bilinmektedir), [(ö-klorpiridin-S- bilinmektedir), [1-(6-klorpiridin-3-il)etil](metil)oksid0-A4-sulfanilidensiyanamid (WO Burada tarif edilen diger uygulamalar, içerisinde Bacillus firmus CNCM 1-1582 sporu yerine (C1), (C2), (C1-1) to (C1-10) kompozisyonlarinin herhangi birinde biyolojik kontrol ajaninin B grubunun herhangi bir 'üyesi oldugu kompozisyonlari içermektedir. (C1-9) The biological control agent is Bacillus firmus CNCM 1-1582 spore. and insect control agent combinations of a) chlorantaniliprole, and b) imidacloprid or clotianidine or thiamethoxam or thiacloprid, or acetamiprid or nitenpyram or sulfoxaflor or selected from the following list one of the compounds: 4-{ [(6-brompyrid-3-yl)methyl](2-fluoretyl)amino}furan-2(5H)-one (WO known from documentation), 4-{[(6-chloropyrid-3-yl)methyl](2-fluoretyl)amino}furan-2(5H) - known from documentation), 4-{[(5,6-dichloropyrid-3-yl)methyl](2-fluoretyl)amino}furan- known), 4-{[(6-chloropyrid-3-yl)methyl](cyclopropyl)amino}furan-2(5H)-one (EP-A-O known), [1-(6-chloropyridin-3-yl)ethyl](methyl)oxido-A4-sulfanilidenecyanamide (WO (C1-10) Bacillus firmus CNCM 1-1582 spore of biological control agent in and the insect control agent consisting of: a) cyantraniliprole b) imidacloprid or clotianidine or thiamethoxam or thiacloprid, or acetamiprid or nitenpyram or sulfoxaflor or selected from the following list one of the compounds: 4-{ [(6-brompyrid-3-yl)methyl](2-fluoretyl)amino}furan-2(5H)-one (WO known from documentation), 4-{[(6-chloropyrid-3-yl)methyl](2-fluoethyl)amino}furan-2(5H) - known from documentation), 4-{[(5,6-dichloropyrid-3-yl)methyl](2-fluoretyl)amino}furan- known), 4-{[(6-chloropyrid-3-yl)methyl](cyclopropyl)amino}furan-2(5H)-one (EP-A-O 2(5H)-one (known from EP-A-O 539 588), [(o-chlorpyridine-S- known), [1-(6-chloropyridin-3-yl)ethyl](methyl)oxideO-A4-sulfanilidenecyanamide (WO Other applications described herein include Bacillus firmus CNCM 1-1582 spore in any of the compositions (C1), (C2), (C1-1) to (C1-10) includes compositions in which the control agent is any member of group B.

Baska bir uygulamada, burada tarif edilen kompozisyonlar bir izoflavon içerebilir. In another embodiment, the compositions described herein may contain an isoflavone.

Izoflavonlar, genis bir biçimde Leguminosae bitki familyasinin 'üyelerinde olan bitki kimyasallaridir. Bunlar 'örnegin Carlson et al (1980) Journal of Chromotography, Patentinde tarif edildigi gibi basit bir difenolik halka yapisina dayanmaktadir. Burada tarif edilen bilesende (lt) kullanisli olan izoflavonlarin 'örnekleri, bunlarla kisitli olmamak kaydiyla genistein, biyokanin A, 10 formononetin, daidzein, glisitein, hesperetin, naringenin, kalkon, kumarin, Ambiol(2-metiI-4-[dimetilaminometil]-5-hidr0-, askorbat ve pratensein ve bunlarin tuzlari ve esterlerini kapsamaktadir. Formononetin, hesperetin, naringenin ve bunlarin tuzlari, esterleri ve karisimlari tercih edilen izoflavonlardir. Isoflavones are plant members of the broad plant family Leguminosae. chemicals. These are 'for example Carlson et al (1980) Journal of Chromotography, It is based on a simple diphenolic ring structure as described in its patent. Here Examples of 'isoflavones' useful in the component (lt) described, including but not limited to with the exception of genistein, biochanin A, 10 formononetin, daidzein, glycitein, hesperetin, naringenin, chalcone, coumarin, Ambiol(2-methyl-4-[dimethylaminomethyl]-5-hydro-, ascorbate and pratensein and their salts and esters. Formononetin, hesperetin, naringenin and their salts, esters and mixtures are preferred. are isoflavones.

Tercih edilen bir uygulamada, bir izoflavon, (C1), C(2), (C1-1) ila (C1-10) kompozisyonlari ile karistirilir. In a preferred embodiment, an isoflavone, (C1), C(2), (C1-1) to (C1-10) mixed with its compositions.

Ozellikle tercih edilen bir izoflavon, bir tuz ya da serbest asit olarak formononetindir. A particularly preferred isoflavone is formononetin as a salt or free acid.

Baska bir uygulamada, burada tarif edilen kompozisyonlar bir asi malzemesi, özellikle de bir toprak asi malzemesi içerebilir. Bu asi malzemelerinin örnekleri Rhizobium, Pseudomonas, Azospirillum, Azotobacter, Streptomyces, Burkholdia, Agrobacterium, End0-, Ect0-, Vesicular-Arbuscular (VA)Myc0rhizza cinslerinden bakterilerdir. In another embodiment, the compositions described herein contain a base material, particularly may also contain a soil acid material. Examples of these rebel materials are Rhizobium, Pseudomonas, Azospirillum, Azotobacter, Streptomyces, Burkholdia, Agrobacterium, They are bacteria of the genus End0-, Ect0-, Vesicular-Arbuscular (VA)Myc0rhizza.

Tercih edilen bir uygulamada, bir asi malzemesi (C1), C(2), (C1-1) ila (C1-10) kompozisyonlarindan biri ile karistirilir. In a preferred embodiment, a base material (C1), C(2), (C1-1) to (C1-10) mixed with one of its compositions.

Ayni zamanda, topraga (yani karik içerisine), bitkinin bir kismina (yani sulama) ya da ekimden önce tohum üzerine (yani tohum kaplama veya gübreleme) etkili bir miktarda geleneksel çesitli formülasyonlarin herhangi birinin uygulanmasi yoluyla bir bitkinin tedavi yöntemleri saglanmaktadir. Geleneksel formülasyonlar solüsyonlari (SL), emülsiyon haline getirilebilir konsantre (EC), sulandirilabilir tozlar (WP), süspansiyon konsantresi (SC ve F8), sulandirilabilirtoz (WP), çözülebilirtozlar (SP), granüller (GR), süspansiyon-emülsiyon konsantresi (SE), aktif bilesik emdirilmis dogal ve sentetik materyaller ve polimerik maddelerde çok ince kontrollü salim (CR) kapsüllerini kapsamaktadir. Bir uygulamada, böcek kontrol ajani ve biyolojik kontrol ajani, kullanima hazir bir formülasyon halinde bulunabilen veya kullanim sorasinda karistirilan tozlar seklinde formüle edilir. Her iki uygulamada, söz konusu toz, ekimden önce veya ekim sirasinda toprakla karistirilabilir. Alternatif bir uygulamada, biyolojik kontrol ajani veya böcek kontrol ajaninin biri ya da her ikisi, tedavi sirasinda birbirine karistirilan bir likit formülasyondur. Teknikte siradan uzmanliga sahip olan bir kimse, bulus kompozisyonlarinin etkili bir miktarinin kompozisyonun nihai formülasyonuna ve ayni zamanda isleme tabi tutulacak olan tohumun büyüklügü veya bitkinin büyüklügüne dayali oldugunu anlayacaktir. It can also be applied to the soil (i.e. inside the furrow), part of the plant (i.e. irrigation) or an effective amount onto the seed (i.e. seed coating or fertilization) prior to planting. of a plant through the application of any of the various conventional formulations. treatment methods are provided. Conventional formulations solutions (SL), emulsifiable concentrate (EC), dispersible powders (WP), suspension concentrate (SC and F8), reconstituteable powder (WP), soluble powders (SP), granules (GR), suspension-emulsion concentrate (SE), natural and synthetic impregnated with active compound ultrafine controlled release (CR) capsules in materials and polymeric substances covers. In one embodiment, the insect control agent and the biological control agent are available in a ready-to-use formulation or It is formulated as mixed powders. In both applications, the powder in question is It can be mixed with the soil before or during planting. In an alternative embodiment, biological control agent or one or both of the insect control agents are interrelated during treatment. It is a mixed liquid formulation. One of ordinary skill in the art, an effective amount of the compositions of the invention to the final formulation of the composition and also the size of the seed or the plant to be treated. he will understand that it is based on his size.

Nihai formülasyon ve uygulama yöntemine bagli olarak, mevcut kompozisyonlara ayni zamanda bir ya da daha fazla uygun katki maddesi dahil edilebilir. Mevcut kompozisyonlara tozlar, granüller ya da lateksler formunda karboksimetilselüloz ve dogal ve sentetik polimerler gibi adezifler, örnegin arap sakizi, kitin, polivinil alkol ve polivinil asetat ve ayni zamanda dogal fosfolipidler, örnegin sefalinler ve Iesitinler ve sentetik fosfolipidler eklenebilir. Depending on the final formulation and method of administration, the same One or more suitable additives may also be included. Available carboxymethylcellulose in the form of powders, granules or latexes and adhesives such as natural and synthetic polymers, such as gum arabic, chitin, polyvinyl alcohol and polyvinyl acetate as well as natural phospholipids such as cephalins and leecithins and synthetic phospholipids may be added.

Tercih edilen bir uygulamada, söz konusu kompozisyonlar tekli, stabili bir solüsyon ya da emülsiyon veya süspansiyon seklinde formüle edilir. Solüsyonlar için, aktif kimyasal bilesikler (yani böcek kontrol ajani), biyolojik kontrol ajani eklenmeden önce solventler içerisinde çözündürülür. Uygun sivi solventler petrol esasli aromatikleri, örnegin ksilen, tolüen ya da alkilnaftalenleri, alifatik hidrokarbonlari, örnegin sikloheksan ya da parafinleri, örnegin petrol fraksiyonlarini, mineral ve nebati yaglari, alkolleri, örnegin bütanol ya da gliserol ve ayni zamdan bunlarin eterleri ve esterleri, ketonlar, örnegin metil etil keton, metil izobütil keton ya da sikloheksanon, güçlü bir biçimde polar solventler, örnegin dimetilformamid ve dimetil sülfoksidi kapsamaktadir. Emülsiyon ya da süspansiyon için sivi ortam sudur. Bir uygulamada, söz konusu böcek kontrol ajani ve biyolojik kontrol ajani, ayri sivilar içerisinde süspansiyon haline getirilir ve uygulama aninda karistirilir. Tercih edilen bir süspansiyon uygulamasinda, böcek kontrol ajani ve biyolojik madde, en az iki yillik bir raf ömrü sergileyen kullanima hazir bir formülasyon içerisinde kombine edilir. Kullanimda, söz konusu sivi püskürtülebilir ya da yapraga veya kariga ürünün ekimi sirasinda atomize edilebilir. Sivi kompozisyon topraga, tohumun çimlenmesinden önce ya da dogrudan köklerle temas halinde olan topraga, bunlarla kisitli olmamak kaydiyla damla sulama, sprinklerler, topraga enjeksiyon ya da topragi islatma gibi çesitli tekniklerden yararlanarak verilebilir. In a preferred embodiment, said compositions are used as a single, stable solution or It is also formulated as an emulsion or suspension. For solutions, active chemical compounds (ie, insect control agent), solvents before adding the biological control agent dissolved in it. Suitable liquid solvents are petroleum-based aromatics, eg xylene, toluene or alkylnaphthalenes, aliphatic hydrocarbons such as cyclohexane or paraffins, eg petroleum fractions, mineral and vegetable oils, alcohols, eg butanol or glycerol and also their ethers and esters, ketones, for example methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents include, for example, dimethylformamide and dimethyl sulfoxide. emulsion or Also, the liquid medium for suspension is water. In one embodiment, said insect control agent and the biological control agent are suspended in separate liquids and applied. instantly mixed. In a preferred suspension application, the insect control agent and the biological material is a ready-to-use product with a shelf life of at least two years. combined in the formulation. In use, the liquid in question can be sprayed or It can also be atomized into the leaf or rattle during planting of the crop. liquid composition into the ground before the germination of the seed or directly in contact with the roots to the ground, including but not limited to drip irrigation, sprinklers, It can be given by using various techniques such as injection or soil soaking.

Istege bagli olarak, alkali ve toprak alkali metal tuzlari ve organik asitler, örnegin sitrik asit ve askorbik asit, inorganik asitler, örnegin hidroklorik asit ya da sülfürik asit gibi stabilizörler ve tamponlar eklenebilir. Biyositler de eklenebilir ve formaldehitleri ya da formaldehit salan ajanlari ve benzoik asit türevlerini, örnegin p-hidroksibenzoik asidi içerebilir. Optionally, alkali and alkaline earth metal salts and organic acids, eg citric acid and ascorbic acid, inorganic acids such as hydrochloric acid or sulfuric acid stabilizers and buffers can be added. Biocides may also be added and formaldehydes or formaldehyde releasing agents and benzoic acid derivatives, eg p-hydroxybenzoic acid may contain.

Bir uygulamada, kati ve sivi kompozisyonlar ayrica tohumlari seçici herbisitlerin zararli etkilerinden koruyabilen aktiflestirilmis karbon, besleyiciler (gübreler) ve çimlenmeyi ve ürünlerin kalitesini iyilestirebilen baska ajanlar gibi fonksiyonel ajanlar içermektedir. In one embodiment, the solid and liquid compositions also protect the seeds against harmful effects of selective herbicides. activated carbon, nutrients (fertilizers) and germination and contains functional agents such as other agents that can improve the quality of products.

Ozellikle tercih edilen bir uygulamada, burada tarif edilen kompozisyonlar bir tohum tedavisi olarak formüle edilir. Tohum tedavisi en az bir böcek kontrol ajani ve en az bir biyolojik kontrol ajani içermektedir. Tohumlar burada ifsa edilen kompozisyonlarin bir ya da daha fazla tabakasi ile geleneksel karistirma, püskürtme ya da bunlarin kombinasyonundan olusan yöntemler kullanarak, spesifik olarak dizayn edilmis ve üretilmis tedavi uygulama ekipmani kullanarak, tohum tedavi ürünlerinin tohumlara dogru, güvenli ve verimli bir biçimde uygulanmasi için büyük ölçüde tek biçimli olarak kaplanir. Bu ekipman döner kaplayicilar, tamburlu kaplayicilar, akiskan yatak teknikleri, püskürtmeli yataklar, döner bugular ya da bunlarin bir kombinasyonu gibi çesitli kaplama teknolojilerini kullanir. Burada tarif edilenler gibi sivi tohum tedavileri döner bir “atomizer” disk veya tohum tedavisini tohumlar üzerine sprey paterni hareket ettikçe homojen sekilde dagitan bir sprey nozülü vasitasiyla uygulanabilir. Tercih edilen sekliyle, tohum daha sonra ilave tedavi dagitimi ve kurutma saglamak için ek bir zaman dilimi boyunca karistirilir veya tamburlanir. Tohumlar, çimlenme ve emerjans homojenligini arttirmak için bulus kompozisyonlari ile kaplamadan önce islemden geçirilebilir veya geçirilmeyebilir. Alternatif bir uygulamada, kuru bir toz formülasyonu hareketli tohum üzerine ölçülü sekilde uygulanabilir ve tamamen dagilana kadar karismasi saglanir. In a particularly preferred embodiment, the compositions described herein are formulated as a treatment. Seed treatment with at least one insect control agent and at least one Contains biological control agent. The seeds are one of the compositions disclosed herein. or more layers of conventional mixing, spraying or using methods consisting of a combination of specifically designed and seed treatment products into seeds, using manufactured treatment application equipment. largely uniform for its correct, safe and efficient application. is covered. This equipment is suitable for rotary coaters, drum coaters, fluidized bed techniques, such as spray bearings, rotary mist, or a combination of these. uses various coating technologies. Liquid seed treatments such as those described here A rotating “atomizer” disc or spray pattern moves the seed treatment onto the seeds. It can be applied by means of a spray nozzle that distributes homogeneously as it spreads. Choice As described, the seed is then added to provide additional treatment distribution and drying. it is mixed or tumbled for a period of time. seeds, germination and prior to coating with compositions of the invention to increase emergence homogeneity. may or may not be processed. In an alternative embodiment, a dry powder The formulation can be applied sparingly on the motile seed and completely It is allowed to mix until it disperses.

Tohumlar bir kesikli veya sürekli kaplama prosesi vasitasiyla kaplanabilir. Sürekli bir kaplama uygulamasinda, sürekli akis ekipmani es zamanli olarak hem tohum akisini hem de tohum tedavi ürünlerini ölçer. Tohum akisini bir sürgülü tapa, koni ve açiklik, tohum çarki ya da tartim cihazi (kemer veya saptirici) düzenler. Tedavi ekipmani içerisinde tohum akis hizi belirlendikten sonra, tohum tedavisinin akis hizi, tohum tedavi ekipmani içerisinde geçtikçe istenen dozu tohuma vermek için tohum akis hizina kalibre edilir. Ilave olarak, bir bilgisayar sistemi kaplama makinesine tohum girisini izleyebilir ve bu suretle tohumun uygun miktarinin sabit bir akisi korunmus olur. Seeds can be covered by a batch or continuous coating process. A continuous In coating application, the continuous flow equipment simultaneously circulates both the seed flow. as well as measure seed treatment products. Seed flow with a slide plug, cone and opening, regulates the seed wheel or weighing device (belt or deflector). treatment equipment After determining the seed flow rate in the seed treatment flow rate, the seed the seed flow rate to deliver the desired dose to the seed as it passes through the treatment equipment. is calibrated. In addition, a computer system enters the seed inlet into the coating machine. and thus a constant flow of the appropriate amount of seed is maintained.

Bir kesikli kaplama uygulamasinda, kesikli tedavi ekipmani belirtilen birtohum miktarini tartar ve tohumu kapali bir tedavi haznesine veya teknesine yerlestirir, burada daha sonra uygun tohum tedavi dozu uygulanir. Bu grup daha sonra sonraki grubun islenmesi için hazirlikta tedavi haznesinden bosaltilir. Bilgisayar kontrol sistemleriyle, bu grup prosesi, grup tedavi prosesini sürekli olarak tekrarlamaya olanak verecek sekilde otomatiklestirilir. In a batch coating application, batch treatment equipment can deliver a specified amount of seed. tartar and place the seed in a closed treatment hopper or trough, where Then the appropriate seed treatment dose is applied. This group is then the next group. It is emptied from the treatment chamber in preparation for processing. With computer control systems, this group process will allow to repeat the group treatment process continuously is automated in this way.

Her iki uygulamada, tohum kaplama makine aksami istege bagli olarak, çesitli ekipmanin çalisan müdahalesi olmadan baslatilmasina ve durdurulmasina olanak veren programlanabilir bir mantik kontrolörü tarafindan çalistirilabilir. Bu sistemin komponentleri, Gustafson Equipment of Shakopee, MN gibi çesitli kaynaklardan ticari olarak tedarik edilebilir. In both applications, the seed coating machinery is optionally available in various Allows equipment to be started and stopped without employee intervention. It can be operated by a programmable logic controller. This system Components are commercially available from various sources such as Gustafson Equipment of Shakopee, MN. can be supplied as

Bulus kompozisyonlarini içeren tohum tedavi formülasyonlarina çesitli katki maddeleri eklenebilir. Baglayicilar eklenebilir ve kaplanacak olan tohum üzerinde fitotoksik ektisi olmadan dogal ya da sentetik olabilen bir adezif polimerden olusanlari kapsamaktadir. Various additives to seed treatment formulations containing compositions of the invention can be added. Binders can be added and have phytotoxic effects on the seed to be coated. These include those consisting of an adhesive polymer, which can be natural or synthetic, without

Farkli renklendiriciler, örnegin nitrozo, nitro, azo, örnegin monoazo, bisazo ve poliazo, difenilmetan, triarilmetan, ksanten, metin, akridin, tiyazol, tiyazin, indamin, indofenol, azin, oksazin, antrakuinon ve fitalosiyanin olarak siniflandirilan organik kromoforlardan yararlanilabilir. Eklenebilen diger katki maddeleri demir, manganez, bor, bakir, kobalt, molibden ve çinko tuzlari gibi eser miktarda besleyicileri kapsamaktadir. Tohum yüzeyi Diger geleneksel tohum tedavi katkilari, bunlarla kisitli olmamak kaydiyla kaplama ajanlarini, sulandirma ajanlarini, tamponlama ajanlarini ve polisakkaritleri kapsamaktadir. Tohum tedavi formülasyonuna su, katilar ya da kuru tozlar gibi en az bir tarimsal olarak kabul edilebilir tasiyici eklenebilir. Kuru tozlar kalsiyum karbonat, kireç, vermik'ulit, talk, humus, aktif kömür ve çesitli fosforöz bilesikler gibi çesitli materyallerden elde edilebilir. Different colorants, eg nitroso, nitro, azo, eg monoazo, bisazo and polyazo, diphenylmethane, triarylmethane, xanthene, methine, acridine, thiazole, thiazine, indamine, indophenol, organic chromophores classified as azine, oxazine, anthraquinone, and phthalocyanine can be used. Other additives that can be added are iron, manganese, boron, copper, cobalt, It contains traces of nutrients such as molybdenum and zinc salts. seed surface Other traditional seed treatment additives, including but not limited to coating agents, wetting agents, buffering agents and polysaccharides covers. At least a minimum of water, solids or dry powders may be added to the seed treatment formulation. an agriculturally acceptable carrier can be added. Dry powders of calcium carbonate, miscellaneous such as lime, vermiculite, talc, humus, activated charcoal and various phosphorous compounds. available from the materials.

Bir uygulamada, tohum kaplama kompozisyonu organik ya da inorganik, dogal veya sentetik bir bilesen olan en az bir dolgu maddesi içerebilir ve bununla aktif bilesenler, bunun tohum 'üzerine uygulanmasini kolaylastirmak için kombine edilir. Tercih edilen sekliyle, dolgu maddesi killer, dogal ya da sentetik silikatlar, silika, reçineler, mumlar, kati gübreler (örnegin amonyum tuzlari), dogal toprak mineralleri, örnegin kaolinler, killer, talk, kireç, kuartz, atapulgit, montmorillonit, bentonit ya da kizelgur veya sentetik mineraller, örnegin silika, alümina veya silikatlar, özellikle de alüminyum ya da magnezyum silikatlardir. In one embodiment, the seed coating composition may be organic or inorganic, natural or may contain at least one filler, which is a synthetic component, and the active components therewith, combined to facilitate its application to the 'seed'. Preferred as filler clays, natural or synthetic silicates, silica, resins, waxes, solid fertilizers (eg ammonium salts), natural soil minerals, eg kaolins, clays, talc, lime, quartz, attapulgite, montmorillonite, bentonite or kieselguhr or synthetic minerals, for example silica, alumina or silicates, especially aluminum or magnesium silicates.

Nematodlar ve/veya patojenik mantarlar tarafindan saldiriya elverisli olan bir bitki olusturmak için çimlenebilen her türlü bitki tohumu, bulusa uygun olarak tedavi edilebilir. Uygun tohumlar kara lahana ürünleri, sebzeler, meyveler, agaçlar, Iifli tek çenekli ve çift çenekli türlerden diger bitkileri kapsamaktadir. Tercih edilen sekliyle, bugday, arpa, çavdar, sorgum, pirinç, kolza tohumu, seker pancari, ayçiçegi, domates, biber, fasulye, marul, patates ve havuç tohumlarini kapsamaktadir. En çok tercih edilen sekliyle, pamuk veya misir (tatli, tarla, tohum veya patlamis misirlik) tohumlar mevcut kompozisyonlarla kaplanir. A plant susceptible to attack by nematodes and/or pathogenic fungi any plant seed that can germinate to form a treatment in accordance with the invention. can be done. Suitable seeds are collard greens, vegetables, fruits, trees, It includes other plants from monocot and dicotyledonous species. In its preferred form, wheat, barley, rye, sorghum, rice, rapeseed, sugar beet, sunflower, tomato, pepper, beans, lettuce, potato and carrot seeds. most preferred as, cotton or corn (sweet, field, seed or popcorn) seeds available covered with compositions.

Mevcut bulusa uygun olarak kompozisyonlar, çevre dostu en az bir biyolojik kontrol ajani ve en az bir böcek kontrol ajaninin tarimsal olarak etkili miktarlari ile karistirildiginda beklenmedik derecede iyi genel bitki canliligi ve verimi sergiler. Bu beklenmedik sonuçlar, biyolojik kontrol ajaninin nematisidal ve/veya fungisidal özelliklerinin ve böcek kontrol ajaninin kök kütlesi arttirma özelliklerinin kombinasyonuna atfedilmektedir. Compositions in accordance with the present invention may contain at least one environmentally friendly biological control. agent and at least one insect control agent in agriculturally effective amounts. When mixed, it exhibits unexpectedly good overall plant vitality and yield. This unexpected results indicate that the biological control agent is nematicidal and/or fungicidal. properties and root mass increasing properties of the insect control agent. attributed to the combination.

Bulus kombinasyonlarinin ayni zamanda özellikle transjenik tohumlarla kullanilabilmesi avantajli olarak görülmektedir ve bu suretle bu tohumdan çikan bitkiler, zararlilara ve patojenlere karsi bir protein ekspresyonu gerçeklestirebilir. Bu tohumun bulus ajanlari ile tedavisi yoluyla, örnegin insektisidal proteinin ekspresyonu yoluyla belirli zararli ve patojenler zaten kontrol edilebilir ve ilave olarak bulus ajanlari ile birlikte sinerjik aktivite takviyesi meydana gelmesi ve bunun da zararli ve patojen infestasyonundan koruma verimliligini daha da arttirmasi sasirticidir. Combinations of the invention can also be found, especially with transgenic seeds. It is seen as advantageous to be able to be used and thus the plants emerging from this seed, can perform a protein expression against pests and pathogens. of this seed by treatment with agents of the invention, for example by expression of insecticidal protein certain pests and pathogens can already be controlled and additionally with agents of the invention concomitant synergistic activity reinforcement, resulting in harmful and pathogenic It is surprising that it further increases the efficiency of protection from infestation.

Bulus ajanlari, zaten tarif edildigi gibi tarimda, seralarda, ormancilikta, bahçe insaatinda veya 'üzüm baglarinda kullanilan tüm türlerden bitki çesitlerinin tohumunu korumak için uygundur. Ozellikle, bu, misir, yer fistigi, kanola, kolza, hashas, zeytin, hindistancevizi, kakao, soya pamuk, pancar (örnegin seker pancari ve yem pancari), pirinç, dari, bugday, arpa, yulaf, çavdar, ayçiçegi, seker kamisi veya tütün tohumu ile ilgilidir. Bulusun ajanlari ayni zamanda önceden tarif edildigi gibi meyve bitkileri ve sebzelerin tohumunun tedavisi için uygundur. Misir, soya, pamuk, bugday ve kanola veya kolza tedavisine özellikle önem verilmektedir. Dolayisiyla, örnegin, misir tohumunun tedavisi için (1) numara kombinasyonu özellikle uygundur. Agents of invention are used in agriculture, greenhouses, forestry, horticulture, as already described. Seed of plant varieties of all species used in the construction or 'vineyards' suitable for protection. In particular, it is corn, peanut, canola, rapeseed, hashish, olive, coconut, cocoa, soybean cotton, beet (eg sugar beet and fodder beet), with rice, millet, wheat, barley, oat, rye, sunflower, sugarcane or tobacco seeds is relevant. Agents of the invention are also fruit plants and plants as previously described. Suitable for the treatment of seeds of vegetables. Corn, soybean, cotton, wheat and canola Particular attention is paid to the treatment of rapeseed or rapeseed. So, for example, corn The number combination (1) is particularly suitable for the treatment of seed.

Zaten tarif edildigi gibi, transjenik tohumun bir bulus ajani ile tedavisi özellikle Önemlidir. Bu, genel olarak, özel insektisidal özelliklere sahip bir polipeptidin ekspresyonunu kontrol eden en az bir heterolog gen içeren bitki tohumlari ile ilgilidir. As already described, treatment of the transgenic seed with an agent of the invention is particularly It is important. In general, it is a polypeptide with specific insecticidal properties. relates to plant seeds containing at least one heterologous gene that controls its expression.

Transjenik tohumdaki heterolog gen Bacillus, Rhizobium, Pseudomonas, Serratia, Trichoderma, Clavibacter, Glomus ya da Gliocladium gibi mikroorganizmalardan kaynaklanabilir. Mevcut bulus, Bacillus sp.°den kaynaklanan en az bir heterolog gen içeren ve gen ürünü Avrupa misir kurdu ve/veya bati misir kök kurduna karsi aktivite sergileyen transjenik tohumun tedavisi için özellikle uygundur. Ozellikle, Bacillus thuringiensis'ten kaynaklanan heterolog bir gen tercih edilmektedir. The heterologous gene in the transgenic seed Bacillus, Rhizobium, Pseudomonas, Serratia, Microorganisms such as Trichoderma, Clavibacter, Glomus or Gliocladium can be caused. The present invention has at least one heterologous gene originating from Bacillus sp. activity against European corn borer and/or western corn rootworm It is particularly suitable for the treatment of transgenic seed exhibiting Specifically, Bacillus A heterologous gene from thuringiensis is preferred.

Bakteriyel sporlar bir kimyasal böcek kontrol ajaninin varliginda sasirtici bir biçimde kendi nematisidal ve/veya fungisidal özelliklerini korumanin yaninda, yüksek bir bitki kök sistemini kolonize etme yetenegi de sergilemektedir. Bu yüksek yetenek, bunlarin nematisidal ve/veya fungisidal aktivitesinin artmasina yol açmakta ve böylelikle iyi verimle sonuçlanan iyi canliliga yol açmaktadir. Bacterial spores surprisingly grow in the presence of a chemical insect control agent. In addition to preserving its nematicidal and/or fungicidal properties, a higher plant It also exhibits the ability to colonize the root system. This high talent leads to an increase in nematicidal and/or fungicidal activity and thus a good It leads to good vitality resulting in yield.

Mevcut bulusun avantajlari, asagidaki kisitlayici olmayan örneklerin tarifinden anlasilacaktir. Asagidaki örnekler mevcut bulusun yalniz tercih edilen bir uygulamasini sunmaktadir. Asagidaki örneklerde ortaya konuldugu gibi, mevcut bulusa uygun olarak kompozisyonlar, çevre dostu en az bir biyolojik kontrol ajani ve en az bir böcek kontrol ajaninin tarimsal olarak etkili miktarlari ile karistirildiginda beklenmedik derecede iyi genel bitki canliligi ve verimi sergiler. Bu beklenmedik sonuçlar, biyolojik kontrol ajaninin nematisidal ve/veya fungisidal özelliklerinin ve böcek kontrol ajaninin kök kütlesi arttirma özelliklerinin kombinasyonuna atfedilmektedir. Asagidaki örneklerde ayrica ortaya konuldugu gibi, bakteriyel sporlar bir kimyasal böcek kontrol ajaninin varliginda sasirtici bir biçimde kendi nematisidal ve/veya fungisidal özelliklerini korumanin yaninda, yüksek bir bitki kök sistemini kolonize etme yetenegi de sergilemektedir. Bu yüksek yetenek, bunlarin nematisidal ve/veya fungisidal aktivitesinin artmasina yol açmakta ve böylelikle iyi verimle sonuçlanan iyi canliliga yol açmaktadir. The advantages of the present invention derive from the description of the following non-limiting examples. will be understood. The following examples illustrate only one preferred embodiment of the present invention. offers. In accordance with the present invention, as set forth in the following examples, The compositions contain at least one environmentally friendly biological control agent and at least one insect control agent. Unexpectedly good when mixed with agriculturally effective amounts of the agent exhibits general plant vitality and yield. These unexpected results are root of the nematicidal and/or fungicidal properties of the insect control agent. attributed to the combination of mass increasing properties. In the examples below As has also been demonstrated, bacterial spores are the result of a chemical insect control agent. surprisingly its nematicidal and/or fungicidal properties The ability to colonize a higher plant root system, as well as to protect exhibits. This high ability allows them to be nematicidal and/or fungicidal. It leads to an increase in its activity and thus leads to good vitality resulting in good yield. opens.

Deneyler, belirli bakterilerin kök sistemlerini kolonize etme yetenegini göstermek üzere dizayn edildi. Bu belirli deneyde, hem tedavi edilmemis pamuk tohumlari hem de Bacillus firmus (biyolojik bir nematisid) sporlari ile tedavi edilmis pamuk tohumlari, dogal florayi minimize etmek için otoklavli topraga ekildi. Fideler üç hafta sonra hasat edildi. Steril su ve bir korsa kullanarak, kök sistemleri bakterileri geri kazanmak için isleme tabi tutuldu. Experiments were used to demonstrate the ability of certain bacteria to colonize root systems. was designed. In this particular experiment, both untreated cottonseeds and Cotton seeds treated with spores of Bacillus firmus (a biological nematicide), planted in autoclaved soil to minimize native flora. Seedlings are harvested after three weeks was done. Using sterile water and a cork, root systems to restore bacteria was subjected to heat.

Tüm örnekler çoklu bakteri türü içermesine karsin, B. firmus yalniz tedavi edilen kökten yetisen bitki kök sistemlerinden izole edildi. Bu deney, bir kök tedavisi olarak kullanildigi zaman, B. firmus*un rizosfer içerisinde yetisebildigi ve çogalabildigini ortaya Ornek 1*deki deney daha sonra degistirilmis bir geri kazanma yöntemiyle gerçeklestirildi. Hasat sirasinda, tedavi edilen kökten kök sistemlerinin yarisi 30 saniye boyunca steril suyla durulandi ve bir korsa kullanmak yerine, tüm kök sistemi dogrudan bir triptik soya agar plakasi üzerine yerlestirildi. B. firmus tekrar tedavi edilmeyen örneklerden geri kazanilmadi ve tedavi edilen tohumdan yetisen bitkilerin durulanmamis kök sistemlerinden geri kazanilirken, tutarli bir biçimde geri kazanilan baskin bakteriyel türler degildi. Bununla birlikte durulanan kök sistemlerinde, B. firmus sadece geri kazanilmadi, ayni zamanda tutarli bir biçimde baskin bakteri türleri oldugu ortaya konuldu. Bu deney, bir kök tedavisi olarak kullanildigi zaman, B. firmus'un rizosfer içerisinde yetisebildigi ve çogalabildigini ortaya koymanin yaninda, aslinda kök sistemlerini kolonize edebildigini ortaya koydu. Benzer deneyler ayni zamanda kök kolonizasyonunu ortaya koymak için tarimsal olarak yararli baska bakterilerle de gerçeklestirildi. Although all specimens contained multiple bacterial species, B. firmus was derived from the treated root alone. isolated from growing plant root systems. This experiment is used as a root treatment. When used, it was revealed that B. firmus can grow and multiply in the rhizosphere. The experiment in Example 1 was then followed by a modified recovery method. carried out. During harvest, half of the root systems from the treated root take 30 seconds. rinsed with sterile water throughout and, instead of using a cork, the entire root system was directly placed on a tryptic soy agar plate. B. firmus untreated not recovered from the specimens and the plants grown from the treated seed recovered from unrinsed root systems, while being recovered on a consistent basis were not the dominant bacterial species. However, in rinsed root systems, B. firmus not only recovered, but also consistently dominant bacterial species revealed. When this experiment is used as a root treatment, B. firmus In addition to revealing that it can grow and multiply in the rhizosphere, in fact, root proved to be able to colonize their systems. Similar experiments also with other agriculturally useful bacteria to reveal the colonization of carried out.

Bu deneyin durulanmis kök sistemlerinden geri kazanilan bakterilerin orijinal tedavide kullanilanla ayni türler ve sus oldugunu daha fazla dogrulamak için, 500 baz çifti DNA analizi ve RNA karsilastirmalari gerçeklestirildi. Bu testin sonuçlari geri kazanilan bakterilerin ayni türler olmanin yaninda, tohumun tedavisinde kullanilan bakterilerden ayirt edilemeyen bir RiboPrint paternine sahip oldugunu gösterdi. Bacteria recovered from rinsed root systems of this experiment were used in the original treatment. To further confirm that it is the same species and strain as used, 500 base pairs of DNA analysis and RNA comparisons were performed. The results of this test are recovered In addition to being the same species of bacteria, it is one of the bacteria used in the treatment of the seed. showed that it had an indistinguishable RiboPrint pattern.

Verim verileri analiz edilirken dikkate alinacak pek çok faktör bulunmaktadir ve çevresel kosullar ve farkli hastalik/nematod/böcek sikintilarinin varligi veya yoklugu ayni alan içerisinde bile dalgalanma gösterdiginden karsilastirmali çalismalar güç olabilmektedir. Degiskenlik olmasina karsin, yeterince büyük bir veri setine bakarak, modeller ortaya çikmaya baslar. There are many factors to consider when analyzing yield data and environmental conditions and presence or absence of different disease/nematode/insect nuisances Comparative studies are difficult as it fluctuates even within the same area. can happen. Despite the variability, looking at a large enough dataset, models begin to emerge.

Tablo 3, 10 alan denemesinin ortalamalarini göstermektedir, burada bir kimyasal fungisit kontrol (baz) ve bir biyolojik fungisit, bir neonikotinoid insektisid ve hem biyolojik fungisit hem de neonikotinoid insektisidin bir kombinasyonu ile baz tedavi arasinda verim karsilastirilmistir. Tablo 3 ayni zamanda, bu denemelerin, bilinen nematod infestasyonu alanlarinda gerçeklestirilmis olmasi ve biyolojik fungisit yerine biyolojik bir nematisid kullanilmis olmasi disinda benzer bir protokolden 7 alan denemesini kapsamaktadir. Bu 17 deneme, 2007 yilinda bu iki protokolden toplanan verilerin tümünün ortalamalarini kapsamaktadir. denemesinin ortalamasi denemesinin ortalamasi Verim /BU % iyilesme Verim / BU % iyilesme FC 60.84 FC 41.48 FC = Fungisit Kontrol, BF = Biyolojik Fungisit, BN = Biyolojik Nematisit, NI = Neonikotinoid insektisit Sinerji için Colby formülünden alindan bir esitlik kullanilarak ("Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", by 8. R. Colby, April 11, 1966, Scientific Article No. A 1271 Maryland Agricultural Experiment Station, Department of Agronomy, University of Maryland, College Park, Maryland makalesinde bulunmaktadir), biyolojik kontrol ajanlari ve neonikotinoid insektisitlerin (E) kombinasyonundan verimdeki beklenen yüzdelik artis, tek basina biyolojik kontrol ajanlarinin kullanilmasindan elde edilen verimdeki yuzdelik artis (Pi) ve tek basina neonikotinoid insektisit kullanilmasindan elde edilen verimdeki yüzdelik artis (P2) kullanarak hesaplanir. Table 3 shows the averages of 10 field trials, where a chemical fungicide control (base) and a biological fungicide, a neonicotinoid insecticide and both Base treatment with a combination of biological fungicide and neonicotinoid insecticide yields were compared. Table 3 also shows that these trials carried out in nematode infestation areas and instead of biological fungicide. 7 fields from a similar protocol except that a biological nematicide was used includes the experiment. These 17 trials were collected from these two protocols in 2007. includes the mean of all data. average of experiment average of experiment Yield / THIS % improvement Yield / THIS % improvement FC 60.84 FC 41.48 FC = Fungicide Control, BF = Biological Fungicide, BN = Biological Nematicide, NI = Neonicotinoid insecticide Using an equation from Colby's formula for synergy ("Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", by 8. R. Colby, April 11, 1966, Scientific Article No. A 1271 Maryland Agricultural Experiment Station, Department of Agronomy, University of Maryland, College Park, Maryland article), biological control agents and neonicotinoid insecticides. Expected percent increase in yield from combination (E) versus biological control alone percent increase in yield (Pi) from the use of agents and alone Percent increase in yield from using neonicotinoid insecticide (P2) is calculated using

Bu esitlik yukaridaki denemelere uygulandiginda, fungisit denemelerinde kombinasyon tedavisi için beklenen yüzdelik artis % 1.99 iken (bununla birlikte gerçek artis % 3.93 idi) ve nematisit denemeleri için kombinasyon tedavisindeki beklenen yüzdelik artis % 4.95 iken (gerçek artis % 5.27 idi). When this equation is applied to the above trials, the fungicide trials The expected percent increase for combination therapy was 1.99% (however, the actual increase was 3.93%) and expected in combination therapy for nematicide trials percent increase was 4.95% (actual increase was 5.27%).

Asagidaki istemlerde kullanilan sekliyle, “bir”, “bu” ve benzeri gibi tanimliklar, takip eden nesnenin tekil veya çogul formunu gösterebilmektedir. As used in the following claims, definitions such as "one", "this" and the like, follow It can show the singular or plural form of the object.

Claims

REQUESTS

1. It is a composition containing Bacillus firmus CNCM 1-1582 spore and a fungicide. wherein said fungicide is selected from fluopyram, fluoxastrobin, fludioxonil, iprodione, mankozebr.

2. A composition according to claim 1, characterized in that; It also contains an insect control agent.

3. Compositions according to any one of claims 1 to 2, characterized in that; wherein the insect control agent is selected from the following list: clotianidin, imidacloprid, thiacloprid, thiamethoxam, acetamiprid, methiocarb, thiodicarb, beta-ciyfluthrin, ciyfluthrin, deltamethrin, teflutrin, indoxacarb, spinosacarb, spinosad, thiamethoxam, spinetoram, phytocarb, thiodicarb, beta-cilyfluthrin, ciyfluthrin, deltamethrin, teflutrin, indoxacarb, spinosacarb, spinosad, thiaclopride, thiamethoxam, spinetoram, phyllocarb, benzopyramide, benzopyramide, benzopyramidine, , spiromesiphene, spirotetramate, flubendiamide, (R),(S)methylsulfonylethyl)phthalamide, chlorantraniliprole (Rynaxypyr), or Cyantraniliprol (Cyazypyr), sulfoxafluor, 4-{[(6-br0mpiride-3-yl)methyl](2- fluoretyl)amino}furen-2(5H) - known from ten), 4-{[(6-chlorpyrid-3-yl)methyl](2-fluoretyl)amino}furan-2(5H) - known from document), 4 -{[(5,6-dichloropyrid-B-yl)methyl](2-fluoretyl)amino}furan- known), 4-{[(6-chloropyrid-3-yl)methyl](cyclopropyl)amino}furan- 2(5H)-one (known from EP-A-O 539 588), 4-{[(6-chloropyrid-3-yl)methyl](methyl)amino}furan- known), [1-(6-chlorpyridine-3) -yl)ethyl](methyl)oxido-)\4-sulfanilidenecyanamide (WO

4. It is a treatment method and its feature is; comprising applying a composition of any one of claims 1-3 to a seed, plant, or soil where a plant is growing or in which a plant is desired to grow.

5. It is a seed treatment formulation, its feature is; comprising the composition of any one of claims 1-3.

6. It is a spray formulation for irrigation or application into the furrow, and its feature is; The composition of any one of claims 1-37.

7. It is a herbal treatment method, its feature is; According to any one of claims 1-3, providing at least one composition containing at least one spore-forming bacteria at a ratio of 0.0001 to 20 °/o 0.0001 to 20 % by weight and at least one fungicide at a ratio of 0.01 to 20% by weight/wt., and applying the composition to the plant. .

8. It is the method in claim 7, its feature is; wherein said composition is applied to soil, a seed, fruit and/or a plant or part of a plant.

9. It is the method in claim 8, its feature is; wherein said seed is selected from the group consisting of soybean, wheat, barley, rice, rapeseed, sugar beet, tomato, bean, carrot, cotton and corn seed.

10. The method of claim 8, characterized in that; wherein said composition is applied to the root of a plant.

11. The method in claim 8, and its feature is; is the direct application of said composition to the soil, before the germination of the seed and/or to the soil in contact with the root of a plant or where a plant is desired to be grown.