TR201815947T4 - Biological material processing apparatus. - Google Patents

Abstract

The present invention is an apparatus (1) for treating biological material, comprising: a holder (120) for a container (160, 170, 730) comprising at least one container portion of increased recognition for receiving the biological material; a radiation source (810b) in the respective holder for illuminating the recording area associated with the container portion with increased recognition; a sensor 810a for measuring the radiation intensity from the recording area; and an evaluation unit equipped to record the presence or absence of the container by comparing the measured density to the defined threshold.

Description

DESCRIPTION BIOLOGICAL MATERIAL PROCESSING APPARATUS The present invention is intended to process material, preferably biological material. relates to an apparatus. For example chemistry, biology, medicine or in various technical fields such as environmental science, biological analysis, processing or use of materials (e.g. liquids) must be reacted. For this purpose, liquids or materials are filtered, cooled, heated, separated, washed or pipetted in various ways, or processed by other methods. Often the biological material a long and complex series of process steps to prepare must be applied. In addition, in many cases different large assemblies containing materials in the same series should be processed in the same way or sequences of the same material should be processed in parallel. should be wet. This can take time, limits efficiency and is prone to interruption.

Processing biological materials, eg nucleic acids or extraction of biomolecules such as proteins and/or used in the purification field. For example, biomolecules pertaining to purification. well-known. a method, a 'biological providing access to sample (“lysis”) content, biological sample their contents to a solid support or carrier material. selective coupling (“binding”), the solid state of undesired components removal of support or carrier material (“washing”) and to the separation (“elution”) stages of the desired components is based.

desired absorption during purification of biomolecules. and, for example, silica to allow deabsorption consisting of gel and on the one hand the filter element of the liquid porous or matrix-like structure, on the other hand, the specific or specific a special surface that has a surface to which it is bonded in a process that is not filter elements have been developed. Further purification biomolecules in filter elements only The size is retained by the effect of exclusion. For example, nucleic acid A liquid containing a biomolecule such as the relevant biomolecules or fragments in any case in the filter element. while the remainder is removed from the filter element. In addition, to obtain biomolecules from the filter element, nuclease-free separation fluid such as water, biomolecule passes through the filter element to separate it. requested in this biomolecule is released (separated) from the filter element and in a container is caught. The filter elements in question are often used as an input. as individual containers with opening and outlet opening arranged or arranged in multi-well plates designed as membranes. The filter elements in question centrifugation (rotation format) or vacuum technology It is processed using an apparatus based on a membrane, a having an entrance opening and an exit opening and a individual vessels, columns, centrifuge columns, filter vessels, chromatography columns, columns, columns, rotary columns or single-turn columns Also known in form.

In general, compared to vacuum-based methods, centrifugation The advantages of the process are a higher degree of purity, more high concentration. and low risk of cross contamination.

In general, in terms of quality and concentration The best results for the purification of biomolecules, Centrifuge operating in the high gravity field (> 10,000 X 9) are obtained with columns, which has a minimum cross maximum contamination and the desired substance from the membrane. back allows for gain. However. a disadvantage of this situation, manual operation of centrifuge columns requiring intensive labor This means that different samples are subject to simultaneous processing. the risk of error and processing if kept or processed. increases the duration. Using multi-box plate formats with a higher degree of standardization and automation faster job production can be realized. However, this quality and/or sacrificing quantity.

QIAGEN contains different of the “bind-wash-separate” procedure for biomolecules comprehensive purification based on the fundamental principle Provides procedures and necessary products. For this purpose, different filter materials and equipment eg WO 03/040364 or US It is used as described in the documents numbered 6.277.648.

Description of a typical commercially available purification sequence The product "QIAGEN QIAprep Spin Miniprep Kit" qualified use in a centrifuge with reagents and buffers standardized QIAprep columns and 2ml Supports collection containers.

Various issues related to the processing of biological materials publications are available. For example, the patent numbered US 6.060.022 automatic centrifuge for sample processing in the document An automated system is described. US 5,166,889 in the patent document no. multiple collection cabinets on the conveyor wheel for access A collection system is described in which it is located. BASE A general method for automatic centrifugation containing biological material such as nucleic acids in a centrifuge a method for processing liquid samples in the patent document apparatus is described. In the patent document numbered WO 00/38046, a description of an automatic apparatus for loading the centrifuge the centrifuge columns in question are automatically It starts to operate using the guidance system. EP With reaction vessels in the patent document number 122772 A chemical manipulator for use is described. GB In the patent document numbered 2235639, it is stated that surrounding the rotating bowl A centrifuge with a protective casing is described.

A disadvantage of the existing automation system is that these systems high standard centrifuge columns of the processes it supports and partial manual intervention or manual intervention. It cannot work simultaneously without it.

Patent document EP O979999A2 with an analytical instrument the instrument in question, the container shelves, the instrument in various types and sizes for A test tube containing capped and uncapped test tubes and attachments. In said sample carrier, an infeeder, a Out feeder with out feeder and in feeder There is a cross feeder in between. USS879628A patent document, such as blood samples for hemostasis with a method and apparatus for handling samples is relevant. Patent document WO 97/26541 A1, punctureable for withdrawing a sampling tube containing a cap, and with sample tubes placed radially and in the suction position closed tube containing a cyclic tube carrier in which relates to the sampling apparatus.

The present invention addresses the problems outlined above. it aims to solve at least some of it. This object is in accordance with claim 1 by an apparatus for processing biological material according to is performed.

Additional advantages, features, aspects and aspects of the present invention preferred embodiments of the present invention in detail and certain aspects of it are set forth in the appended claims, specification and drawings. will be clearly visible.

One aspect of the invention is a container for receiving biological material. It relates to a gripping unit for holding. Aforementioned in the container, in the open position. and in the off position. a cover that can be held is found. The gripper unit tries to grip and release the container. a gripper and cover for a rough defined a cap holder for holding it in position.

The defined position is the open position of the cover.

One aspect of the invention is a method for processing biological material. with an apparatus comprising a gripper unit and a centrifuge. is relevant. The gripping part of the gripper unit, cabinet for placing in the centrifuge and removing it from the centrifuge suitable.

One aspect is to transfer a container to a container holder and from a container holder. relates to a process for removal, in which container, a container attached to the cabinet for closing and opening the cabinet. cover - The process in question is a cabinet with a gripper unit grasping or holding; a defined container attached to the cover in position. be held and covered with the cabinet gripper unit. guiding it into the holder and removing it from the container holder, the lid Maintaining the container's defined position contains the steps.

One direction, using a container gripper unit. from the cup holder relates to a process for removing said container, container It includes a lid that attaches to cover its mouth. Aforementioned process, cabin gripping with a gripper unit; cover, coarse cabin to be held and gripped in a connected defined position, by removing the cap from the cup holder using the gripper maintaining its defined position in the container contains the steps.

Another way is to attach it to a container holder using a gripper unit. relates to a process for container insertion, the container in question is a piece that is attached to the container to close the lid of the container. Includes cover. The process in question is a gripper unit. cabin retention; the cover has a defined position and the cabin held, gripper by placing the lid on the container using includes the steps of maintaining the defined position it does. Optional process cabinet with gripper It includes the additional step of releasing it.

Another aspect of the invention is the use of a container first container holder. from the first holding position to the second cup holder regarding the transmission of the pertaining to a process and said container, container mouth includes a lid fitted for closure.

The process is in the first holding position using the gripper unit. cabin grip; cover in a defined position attached to the container. keeping; first container via gripped cabin gripper removing it from the holder or first holding position; grasped using the cabinet gripper to the second cup holder or to the second includes the steps of placing it in the holding position. In the removal and installation stages, the cover is position is maintained. Optional process cabin, includes the additional step of being released with the gripper Another aspect is the container mouth to receive biological material. container for a container fitted with a lid for closing It's about the holder. Cup holder, use in centrifuge at least one holding element for holding the container and contain at least one valve receptor to hold the lid attached to the container the receptor in question is a rough-receptive will allow access to the cover with the cover holder of the unit. it has shape.

Another aspect, biological, is the container for processing the material. pertaining to an apparatus comprising a holder and a centrifuge, the container the holder is intended for use in the centrifuge.

Another direction is for the processing of biological material. relates to an apparatus containing the following parts: at least one container to hold the container for the biological material rotatable centrifugal rotor and container containing holder a gripper for insertion.

Another direction is for the processing of biological material. relates to an apparatus containing the following parts: at least one container for holding the container for biological material rotatable centrifugal rotor and container containing holder, container a gripper for removing it from the holder.

Another direction is for the processing of biological material. relates to an apparatus containing the following parts: at least one container to hold the container for the biological material rotatable centrifugal rotor with holder and a cup holder the container in the first holding position, the second holding of the container holder a gripper for transferring it to its position.

Another aspect is a cabinet centrifuge through a gripper. a guide for placing it in the cup holder on the rotor related to the process and said container holder, container holding is in position. The invention includes the following steps: the gripper is brought into a position relative to the holding position; the gripper moves to place the container in the holding position. is carried; the container is released by the gripper.

Another aspect is a cabinet centrifuge using a gripper. by a process of removing it from the cup holder on the rotor related and the said cup holder is in the container holding position.

The process in question includes the following stages: gripping, holding is brought to a position relative to its position; container with gripper is grasped; gripper about to remove container from holding position is moved.

Another direction, the cabinet through a gripper, centrifuge from the first holding position of a cup holder on the rotor to the second holding position of the cup holder on the centrifuge rotor relates to a process of transmission. Current invention, water includes stages: gripper attached to first holding position is brought into a position; the container is grasped by the gripper; the gripper moves to pick up the container in the first holding position is carried; gripper, attached to the second holding position is brought; gripper, placing the container in the second holding position moved for the purpose; the container is released by the gripper is left.

Another aspect is water for processing biological material. relates to an apparatus containing parts: a rotor axis rotating centrifugal rotor The drive mechanism adjusts the angular position of the centrifugal rotor. a counter which can rotate with the rotor to position with rotor in interaction with element positioning a non-rotating positioning element.

Another aspect is that the centrifugal rotor of a centrifuge angular rotation of the rotor about the axis of rotation It relates to a process of positioning. Aforementioned The process includes water stages: with centrifugal rotor with a rotatable positioning counter element, centrifugal rotor interacting with a non-rotating positioning element is brought into the operating range to enter and the centrifugal rotor, positioning counter element and positioning element positioned by interaction.

Another direction is for the processing of biological material. relates to an apparatus containing the following parts: container for a container for receiving biological material a rotor axis suitable for mounting the holder axially rotating centrifugal rotor, axial the first stop for movement and the container holder with the positioning counter element. deviation angle in case of impact suitable for holding the cup holder against the stop positioning element. Another aspect is that biological material to the centrifuge rotor in relation to a container for deviation angle of an axially mounted cup holder It relates to a process for positioning. Aforementioned The process includes the following steps: a positioning element, interacting with the positioning countermember of the cup holder enters; cup holder to axial movement of the cup holder contacts a stop for the deflection angle, positioning. element and against positioning It is positioned in the stop part by the interaction of the element.

Another aspect is a container for receiving biological material. relates to the carrier. Cup holder in a centrifuge holder for holding a container, suitable for use with a shape that will snap into a positioning element contains a positioning counter element. Thanks to this hook angular position and/or deflection angle of the cup holder positioning is possible.

The actual stop is generally the cup holder's It is mounted in the centrifuge chamber where it is placed.

Biological material processing apparatus water according to the present invention Contains parts: a container for receiving biological material holder for at least one container with increased recognizability container with part; for heating a recording area in the holder connected to the container area with increasing recognition and radiation field; the radiation intensity from the recording area. a sensor for measuring; with a defined threshold recording the absence or presence of containers by comparison An evaluation unit designed for

Another aspect is in a holder for said container. the presence of a container for receiving biological material, or your absence. It relates to a process for detecting. Promise The process in question includes the following steps: to increased recognition the recording area in the holder associated with the container part is illuminated; radiation intensity from the recording area measured; the presence or absence of a vessel is measured by density recorded by comparison between a defined threshold value.

Another aspect is water for processing biological material. relates to an apparatus containing parts: biological container holder for receiving a container for material; a a sensor unit with an ultrasound source and an ultrasound sensor and the sensor data of the ultrasound sensor in question. determine the state of the container mouth as a function and a function of the sensor data of the ultrasound sensor when on to determine the possible presence of the substance in the container. evaluation unit.

Another aspect is a process for the processing of biological material. The apparatus contains water parts: a vessel feed hopper station for holding; cap on hopper station a sign containing information on the nature of the containers and a radiation sensor for reading the sign.

Another direction is at the hopper station for said container. determining the nature of the container for the collection of biological material relates to a process for The process water contains the steps: containing information about the cabin quality the first sign on the hopper station is illuminated; first the radiation intensity from the beacon is measured; container nature, recorded as a function of the first density measured.

Another aspect is the use of a centrifuge. relates to the process, each of which is a biological material a plurality of container holding positions for the container. Available The process includes the following stages: for container holding positions registers whether a cabinet is available; cap holding The total number of containers recorded with the expression present at their location the number is recorded; available in container holding positions based on the total number of containers recorded. to the container holding positions of the containers. at least one distribution the condition is determined; available in container holding positions conformity of the distribution of the registered containers to the condition in question. a check is performed. Another direction, promise relating to an apparatus for performing the processes in question in addition to carrying out the individual process stages. Contains associated apparatus parts. These process steps a computer with hardware components, using the appropriate software program, a combination of the two, or any other method realizable. The present invention is additionally described above. It is directed to the processes in which the apparatus is running. Aforementioned process for performing each function of the apparatus contains the steps.

Some aspects are shown in the figures, and from this section is explained in more detail. In drawings: Figure la-c, without gripper unit and pipette discharge top view of the work plate with station and magnified top view of the pipette discharge station and centrifugal cover open operation with gripper unit shows the top view of the plate; Figure 2a, b shows the working plate of an arrangement in Figure lc. and shows a perspective view of a slot; Figure 3a-c cover. in the off state; with the lid open; your cover perspective of centrifuge unit with blade shows its appearance; Figure 4, lateral transverse of the centrifuge unit in Figure 3a shows the cross section; Figure 5a-c is a suspended one in the centrifuge rotor of the centrifuge. to the maximum pivot angle of the pot holder and pot holder shows a stop for Figure 6, the gripper with a gripper and pipetting unit shows the perspective view of the slider; Figure 7, perspective of the pipetting unit in Figure 6 shows its appearance; Figure 8, perspective of the gripper unit in Figure 6 shows its appearance; Figure 9 shows that the gripper of the gripper unit in Figure 8 shows the perspective view; Figure 10 is a perspective view of the container holder. shows.

Side view of Figure lla-d cup holder shows; Figure 12, consumables for consumables shows a perspective view of the material station; Figure 13, perspective of the unit on heating and agitation shows its appearance; Figure 14a, b of a unit for heating and agitation side view and top view respectively shows.

Figure 15 shows the sensor functions and control simple diagrammatic representation of functions shows.

Figure 16 shows the sensor functions and control detailed diagrammatic representation of functions shows.

Figure 17a, b user guide picture screens shows; Figure 18a, b users can choose a procedure, prepare and pictorial displays that guide you to operate shows.

Basic concept Biological materials described from this chapter apparatus for processing, process or biological material efficient operation of the procedural stages of processing allows or supports it. In the apparatus in question preferably one or more of the following useful aspects is applied.

One of the beneficial aspects is that the material is centrifuged. no centrifugation, as well as process stages, including other stages, without application of some of them in centrifuge. The process in question performing the steps in the centrifuge, other procedure of centrifugal apparatus such as cup holder The advantage of being able to be used efficiently for the stages of it provides. Also in some cases, other than centrifuge equipment may not be used. In addition, centrifuge and centrifuge material transfer can be reduced. In addition, movement distances can be shortened. As a result, time is saved and The risk of cross contamination can be minimized.

For performing the procedure steps in the centrifuge as well as the removal of material from a position in the centrifuge, placing the material in one position in the centrifuge and centrifugation It is also beneficial to transfer it between different locations within the

For this purpose, related to material transfer among other methods Two methods are available. First of all, if the material in question is liquid, can be pipetted. For this purpose, a pipetting unit in the apparatus may be available (comparable to, for example, Figure '7). Second In particular, a container containing material may be carried. Promise The container in question may be of the desired type. Preferably, for example a It is a column like a centrifugal column, that is, a single-turn column. or, for example, an Eppendorf tube or similar container. reaction or collection vessel, i.e. a plastic with/without cover caps. This type of vessel is removed from the first position in the centrifuge. convert to second position inside or outside the centrifuge For example, a movable gripper for the container in the apparatus can be found.

These and similar processes for material handling are It can be applied even without the use of a centrifuge.

Grasping a container for receiving biological material, and The gripper is suitable for releasing. Aforementioned gripper, intended to grasp and release a container a movable gripper to engage the gripper a suitable clamping element, a lifting element and lifting lifting element of said lifting element for to initiate its movement and thus to move the gripping element. It includes a drive element suitable for driving.

Optional clamping element, opening the clutch element suitable for clamping. As another option the lifting action of the lifting element moves in the closing direction. Alternative clamping clamp the element in the closing direction of the clutch element. suitable. As an additional option, the lifting element lifting movement, moving the clutch element in the opening direction Said clamping element is used to release the container. optionally suitable for clamping the gripping element. Additional as an option, with the lifting action of the lifting element, the gripping element that grips the container is moved. Alternative as the clamping element, the clutch for the container gripping suitable for clamping the element. As an additional option lifting of the lifting element. movement, the container is free. to leave moves the clutch element.

Typically suitable for attachment to cup ring on coupling element There is an indentation. Coupling element optionally is the first gripping element, and the first gripper in said gripper second clutch with features parallel to the clutch element element is available. In addition, the gripper has a pivot axis It is possible to mount axially around it. In this case the grip element has a central axis for said container. and the pivot axis is different from the central axis.

Another aspect of the invention is the biological use of a gripper. by a process of grasping the container for receiving the material is relevant. The process in question includes: removal creating the lifting movement of the element; lifting in the closing direction of the lifting movement of the to the movement of the gripping element for gripping converting the movement in question to a clamping element orienting it against the force it exerts on the gripping member; and gripping the cabin using said gripping element.

Alternatively, the lifting element in question movement of the gripper, for example, into the container. if the container grips on a steered container surface clutch in the opening direction for gripping said vessel element can be converted to motion.

Another aspect of the invention is the biological to release a cabinet for material collection relates to a process. This process includes water stages. means: the lifting action of a lifting element starting, clutching in the opening direction of the lifting movement a clamping element that allows the movement of the element gripping element for cabin release via starting its movement in the opening direction and the car, the clutch release via element. As an alternaive, The lifting action of the lifting element also can allow the movement of the element in the closing direction and for releasing the container of the gripping element in question. movement in the closing direction by the clamping element can be triggered. Another aspect of the invention is the biological material with a gripper unit for holding a container for receiving is relevant. The gripper unit contains water parts: 2 A gripping element movable in the direction of from the first position to the second position, depending on the gripper element a movable gripper and the gripper first a drive for moving from position to second position element.

Optionally, the gripper unit can be moved to another container. It is designed to be placed in the container. Optional drive The element is an electric drive element. optionally movement of the gripper from the first position to the second position, z takes place in a different direction. optionally movement of the gripper from the first position to the second position, in the z direction takes place in a vertical direction. Optionally, the first A pivot axis that moves the gripper from position to second position It is the rotational motion that takes place around it. Optionally, The pivot axis extends parallel to the z direction.

Another aspect is the sample material, preferably the sample liquid. water, which is intended to automatically process biomolecules and relates to an apparatus containing elements: first of all, each one to take a filter cabinet removable and a first opening for holding and optionally in such a way that it can remove an additional sealed container multiple secondary openings for receiving and holding a rotor involved in holding and rotating the chamber a centrifuge containing; the filter cabinet in question is in question means for receiving from the first disclosure; the filter in question to connect the cabinet with said additional container so that the two cabinets to ensure that it can rotate jointly with the centrifuge and thus the filter a liquid connection between the volume of the cabinet and the additional container at least one transaction containing means for providing unit.

The said processing unit is optionally equipped with the relevant filter. cabinet connected to said second opening from the first opening includes means for bringing into the additional container.

The processing unit can also optionally use the filter in question. cabin or said additional container, said filter cabinet or said sealed coarse coaxial intended to rotate around the car center line in the state includes tools. The filter cabinet in question the means of taking from the first deficit are optionally mentioned. the filter cabin in question is the sealed captain It contains the first motor for separation in the coaxial direction.

Filter cabinet, tools for inserting into additional container optionally to bring the first filter cabinet to the additional container. for at least one second engine.

Another aspect of the invention is the ability to hold biological material. relates to a cognitive unit of managing a vessel.

The container in question can be kept in the open and closed positions. There is a cover. The gripper unit grips and releases the container. a gripper and lid for releasing includes a cap holder for holding it in a defined position. it does. The defined position is the open position of the cover.

Another aspect includes the above-mentioned gripper unit. relates to a system. The relevant system is additionally a contains a centrifuge, the gripper cabinet is connected to the centrifuge. It is suitable for placing or removing from the centrifuge.

The cap is optionally made of plastic. Cover optional as coarse. Optional gripper unit, working perpendicular to the plane of the work plate in the z direction or It moves in the x-y direction along the plane. optionally the container has a circular cross-section, preferably 2 cm in diameter, It is under 1 cm. The lid optionally closes the lid of the container. suitable. Cover optionally with a coarse connector it is attached. Optionally, the container is held by the gripper. into a recess through which the cap connector is inserted when gripped. A clutch element is available. Optional cover the carrier includes a Hßkanik stop for the cap. it does. Optionally, the cover against the mechanical stop pressed with connector. Optional clutch element, cabinet movable to grasp and release. Optional As a container holder, it is a device for handling liquids.

Optional cover holder, clutch cover cover the lid holder will slide along the lid when you lift it or It is configured to be routed. optionally processing involves placing the cabinet in a container holder. The cup holder has a cap receptor and cover holder, cover the cover during the insertion process suitable for placement. Optionally processing, a container includes removing the cabinet from its holder and its holder has a cap receptor; the cover in question retainer in the cap receptor during the removal process. may remove the cover. Optional valve receptor and lid holder is in the same position as the lid attached to the container.

Another aspect of the invention is to transfer a container to a container holder and relates to a process for removing from the container holder, in the container in question, the container for closing and opening the cabinet It has a fitted cover. The process in question is a gripper grasping or holding the cabinet with the unit; your lid is tied to be held in a defined position, with the cabinet gripper unit guiding it into the holder and removing it from the container holder, the lid Maintaining the container's defined position contains the steps.

The process for a cabin transport is optionally cabin, from a cup holder using the gripper unit. process related to the cabinet in question, the container holder or the container. The step of moving it from the holder is still the cabin in question. is to remove it from the cup holder with the gripper. Optionally, step of removing the cover from the cabinet cup holder involves removing the captive from the cap receptor.

Optionally in the cup holder there is a It has a lid receptor and is removed from the cabinet cup holder. The removal step involves removing the lid from the lid receptor. contains and maintains the defined position of the lid attached to the container. Optionally, the process for transporting a cabin, using the gripper unit, the cabinet in question is a container. It is a process for placing the movement to or from the said cabinet cup holder. to the cabinet cup holder in question. includes insertion or insertion. Optional inserting or inserting it into the cabinet cup holder stage of the cap to the cap receptor of the cup holder. includes placement. Optionally, cover During the holding phase, the cover in question is attached to the gripper unit. in a defined position attached to the lid by the lid holder is kept. Optionally, word to place the cover The said cover is released by the gripper unit.

Optionally, preferably more than 2 cm or 1 There is a circular cross-section with a diameter of less than cm.

Optionally, in the container holder, there is a It has a lid receptor and is attached to the cabinet cup holder. The step of placing the cap in question is to the cap receptor. includes the placement of the lid attached to the container and the defined position is maintained.

Another way is to first cap a container using the gripper unit. for transferring from the holder to the second container holder, from the cabinet first cup holder or in the preceding paragraphs the first gripping of the first cup holder as described position and the cabin as described in the previous paragraphs. to the second cup holder or the second cup holder. with a process for placing it in the second holding position is relevant. In particular, said container is located in the first container holder. second holding of the second cup holder from the first holding position transferred to the location. First and second holding positions usually different. First hold position and second hold position in the same container holder or in different container holders. it could be.

Another aspect is the container mouth to receive biological material. container for a container fitted with a lid for closing It's about the holder. Cup holder, use in centrifuge at least one holding element for holding the container and contain at least one valve receptor to hold the lid attached to the container the receptor in question is a rough-receptive will allow access to the cover with the cover holder of the unit. it has shape.

Optionally, the cup holder or holders can be placed in a centrifuge. is mounted. Optionally, cup holder or holders e.g. axially mounted on a centrifugal rotor is mounted axially to the centrifuge. Cover optionally is open, that is, the lid is in the open position.

Another aspect is an apparatus for processing biological material. and contains water parts: to amalgamate biological material suitable for holding a container for a container with a fitted lid for closure holder and said cap in a defined position depending on the container. a lid holder or gripper for the container for holding unit and the container holder in question contains a rough to the cover with the cover holder to hold the attached cover. includes a cover receiver that allows access.

The apparatus is additionally equipped with a centrifuge in said centrifuge. It includes a cup holder suitable for use.

In the container holder or in the two paragraphs before this paragraph Optional access of the lid holder to the lid in the apparatus described as a mechanical contact with the cover holder and the cover. includes. A recess in the cap receptor is optional It allows access to the cover with the cover holder. Promise The apparatus in question is optionally equipped with an apparatus gripper. when grasped, you can easily access the cap with the cap holder. is designed to be.

The cap is optionally made of plastic. Cover optional as coarse. Cover, optionally in cross section it is circular and preferably has a diameter of 2 cm, less than 1 cm.

The lid is optionally suitable for closing a lid opening.

Another direction is for the processing of biological material. relates to an apparatus containing the following parts: at least one container to hold the container for the biological material rotatable centrifugal rotor and container containing holder a gripper for insertion.

Another direction is for the processing of biological material. relates to an apparatus containing the following parts: biological; at least one container to hold the container for the material rotatable centrifugal rotor and container containing holder, container a gripper for removing it from the holder.

Another direction is for the processing of biological material. relates to an apparatus containing the following parts: at least one container for holding the container for biological material rotatable centrifugal rotor with holder and a cup holder the container in the first holding position, the second holding of the container holder a gripper for transferring it to its position. optionally the first and second holding positions are different. first and second holding positions in the same cup holder. or different container may be in their holders.

In these apparatus, the gripper is optional, the protruding container It is designed to grasp the container by passing through the gripper, a a mouth or a main mouth and a protruding lid piece. optional for the lid, the gripper, the container from the mouth of the container grasping one side of a distant piece of lid protrusion is designed to. Optionally the gripping element is a container suitable for attachment to the ring and preferably 'away' from the container mouth. a recess suitable for attachment to one side of the ring.

Said container can optionally receive biological material. oriented. Cup holder optionally axial mounted on the centrifuge rotor. Grabber as optional angular position or deflected position of the cup holder includes a positioning element for identifying it.

Optionally, the said positioning, positioning element interacts with the positioning counter element is performed. To identify a deflected location the positioning element for the container holder with stop, eg with centrifugal rotor to work with a rotatable stop is designed. Gripper, optionally with water parts means: a mobile device for grasping and releasing a vessel. the gripping member is a suitable tool for clamping the gripping member. clamping element, lifting movement of the lifting element to start and thus to move the gripping element. a suitable lifting element. Cab as an option for transfer includes rotary motion. Cup holder optionally can be removed from the centrifuge.

Clamping element, optionally opening the clutch element suitable for clamping. Lifting of the lifting element movement optionally in the closing direction of the clutch element. moves. Clamping element, optionally clutch It is suitable for clamping the element in the closing direction. Lifting lifting action of the element, optionally clutch moves the element in the opening direction. clamping element optionally the gripping element to release the container. can clamp. Lifting movement of the lifting element Optionally move the gripping element to grip the container. it makes. Clamping element optionally gripping the container It can clamp the gripping element. of the lifting element lifting action to optionally release the container moves the clutch element.

Centrifuge with optional movable cup holder mounted on the rotor. In this case the positioning tools optionally the container during insertion / removal / transfer may prevent or restrict the movement of the holder.

Optionally, the transfer is carried out directly. i.e. the gripper is the first removal from the holding position and the second retains the container during the period between insertion into the holding position.

Optional third holding position available during transfer and the apparatus in question is also additionally equipped with the third holding position. transfer between first or second holding position can perform. Clutch element optionally cap at least one recess suitable for engagement with the ring. Clutch element is optionally the first gripping element, The said gripper has features parallel to the first gripping element. having a second gripping element. clutch element optionally axially mounted around a pivot axis is done. The coupling element is optionally a coarse defines the center axis and the pivot axis is separated from the center axis is different.

Another aspect is a cabinet centrifuge through a gripper. a guide for placing it in the cup holder on the rotor It is related to the process and its feature is; said container holder is in the holding position. The invention in question, water stages includes: the gripper is in a position dependent on the holding position. is brought; gripper is moved; the container is released by the gripper is left.

Another aspect is a cabinet centrifuge through a gripper. by a process of removing it from the cup holder on the rotor is related and said cup holder is in the container holding position.

The process in question includes the following stages: gripping, holding is brought to a position relative to its position; container with gripper is grasped; gripper about to remove container from holding position is moved.

Another direction, the cabinet through a gripper, centrifuge from the first holding position of a cup holder on the rotor to the second holding position of the cup holder on the centrifuge rotor relates to a process of transmission. Current invention, water includes stages: gripper attached to first holding position is brought into a position; the container is grasped by the gripper; the gripper moves to pick up the container in the first holding position is carried; gripper, attached to the second holding position is brought; gripper, placing the container in the second holding position moved for the purpose; the container is released by the gripper is left.

Optionally, the above-described processes centrifugal rotor predetermined around its axis of rotation includes the step of adjusting the angle of rotation. Removal and/or insertion gripper and gripping element optionally 2 moves jointly in the direction of the car and the car moves to the second holding position. gripper optionally during positioning it is moved depending on the gripper element. In addition, the promise Onusu processes optionally angular position or deflection Includes the fixing angle step. For example, the cup holder deflection angle, positioning element of the gripper, cup interacting with the positioning counter element of the holder can be fixed by allowing it to enter; pot holder, pot a stop for the rotary movement of the holder contacts and the angle of deflection of the cup holder, positioning on the stop of the positioning counter element with the fixed by interaction. Rotational movement of the container holder It can optionally rotate with the centrifugal rotor. Hold optionally a removable container from the centrifuge is in its holder.

The container gripping stage is the lifting element of a lifting element. initiation of movement; lifting element movement of the clutch element in the closing direction or in the opening to the gripping element movement to grip the container in the direction of It includes the partial stages of converting, feature; said movement, a clamping element directed against the force applied to the grip element and therefore, the cabin is gripped by means of the gripping element.

Cab release stage optional water part contains the stages: lifting in the lifting element to the movement of the gripping element in the opening direction. starting a lifting movement that allows by starting the clutch element in the closing direction or in the closing direction. the cabinet being released by a clamping element and so that said cabin is freed by the gripping element. to be released.

Cab engagement stage, optionally cab one protruding container away from its opening or main opening It includes the cabin grip on one side of its part.

During the positioning of the cabinet, attached to the second holding position gripper optionally in a direction other than 2 it is moved depending on the gripper element. cabin, second gripper during positioning depending on the holding position optionally located in a plane extending perpendicular to the 2 direction it is moved depending on the gripper element. cabin, second during positioning depending on the positioning position The gripper is optionally an axis extending parallel to the 2 direction on the axis connected to the surrounding gripper element is returned. Optional first and second holding positions is different. These are in the same cup holder or in different cup holders. may be in their holders. Cab as an optional grip attached to the protruding side. For material transfer here Controlled liquids for specified processes to allow the transfer and, for example, centrifugation of containers. unwanted movements during automatic transmission. mounted on the centrifuge and centrifuge to ensure It is useful to fix the pot holder. The movements in question around the rotor axis of the centrifugal rotor, among others around the rotation or pivot axis of a container holder rotation of the centrifugal rotor about its axis.

Another aspect of the invention is the use of biological material processing. relates to an apparatus comprising: a rotor centrifugal rotor that can rotate about its axis, centrifuge with a drive element for the rotor and rotor with a rotatable positioning counter element angular position of the centrifugal rotor in interaction a non-rotating rotor with the rotor positioning element.

Determination of angular position optional positioning mechanical element between the positioning counter element and the accomplished by merging. Centrifugal rotor optional a cabinet container for the receipt of biological material as It is convenient to take its holder. Positioning counter element optionally mounted on cup holder or centrifugal rotor is done. Optionally, the apparatus in question is a centrifuge or means for determining the angular position of the centrifuge rotor contains. In this way, the positioning element is positioning element of the positioning counter element An angular shape determined to be in the operating range can be aligned as a position function. positioning element, optionally also as explained later in this section. Suitable for positioning the deflection angle of the cup holder.

Centrifugal rotor optional, to take biological material For axial mounting of a container holder for a container such as suitable for a rotational movement of the container holder. includes a stop element. In this case the positioning element by interacting with the positioning counter element it can hold the cup holder at the deflection angle in the stop element.

The positioning element is a gripper to grip the container. can be attached to the gripping element of the unit. As an alternaive It can also be attached, for example, to a pipetting unit. Positioning, optionally 0.1° of angular position to set accurately allows. The apparatus in question is optionally centrifugal. includes tools for rough positioning of the rotor This allows the angular position to be adjusted with an accuracy of 1.5°. gives. The apparatus in question is additionally optionally centrifugal. for determining the angular position of the rotor, for example 1° to accurately determine the angular position of the centrifuge rotor. includes tools that enable Drive element optional as an electric drive element, preferably an induction motor is an asynchronous motor. positioning element optionally includes a spike the positioning counter element is in this case a contains indentation. Positioning element optionally access to the cup holding position of the cup holder of the gripper is achieved. in such a way that it is possible to position attached to the gripper element. Positioning element optional flexibly mounted on the gripper element in the 2 direction is done. The mounted spring optionally adjusts the cup length It allows the movement of the positioning element on it.

The container is optionally of circular cross-section and preferably it is made of plastic. Positioning element optionally It is arranged by a fixed angular tie in the holding position of the cup holder.

Said apparatus can optionally have multiple, preferably 3 on, more preferably. over 6 or more preferably 10 includes a positioning counter element on it.

Another aspect is that the centrifugal rotor of a centrifuge in the angle of rotation of the rotor around the axis of rotation It relates to a process of positioning. Aforementioned The process includes water stages: with centrifugal rotor with a rotatable positioning counter element, centrifugal rotor interacting with a non-rotating positioning element is brought into the operating range to enter and the centrifugal rotor, positioning counter element and positioning element positioned by interaction.

Optional positioning of the positioning counter element working range for interaction with the element and positioning counter element in the operating range in question around the axis of rotation in the angular range such that It is moved by selecting the angular position of the centrifugal rotor.

Positioning counter element optionally for interacting with the positioning element in stages brought into the operating range: the axis of rotation of the centrifugal rotor angular position around it is detected and positioning element as a function of the detected angular position. the positioning element will be positioned within the working range it is aligned.

By positioning the centrifugal rotor, that centrifuge of the holding position of a container rotation angle of the rotor around the rotor axis optionally detected. The interaction is optionally a mechanical engagement or is the magnetic interaction. centrifuge rotor by positioning rotation angle around the rotor axis optionally determines. The rotation angle of the cup holder in the centrifuge deflection angle of the cup holder with its positioning positioned or fixed.

Another aspect is the following for processing biological material. relates to an apparatus containing parts: a rotor swivel and biological material rotating the container holder for a container centrifugal rotor suitable for mounting a first stop member and container for movement the cup holder against said stop stop by interacting with the positioning counter element a suitable positioning to keep it at a deflection angle against the element.

The rotational motion of the container holder is usually an oscillating motion. or can be identified as a deflection movement because the motion usually defines the angle of deviation.

Positioning, optionally, as previously described here positioning element for angular positioning carried out through Centrifugal rotor optional also caused by the centrifugal force of the centrifugal rotor. a second stop for rotational movement it does. First and/or second stop as optional It is mounted on the centrifuge rotor or it can rotate there.

Positioning optionally against the stop piece obtained by pressing the holder. Stop piece optional caused by the centrifugal force of the centrifugal rotor. rotation of the container holder against a reference rotational movement restricts its movement. Rotation movement large optionally largely takes place around the horizontal axis.

Another aspect is water for biological material processing. relates to an apparatus containing the elements: biological axially move the cup holder for a container to receive material around a rotor axis and suitable for mounting rotatable centrifugal rotor, a positioning counter element the container holder containing the container holder a stop for said container holder and to the stop piece by interacting with the positioning counter element suitable for contacting, the angle of deviation of the cup holder by contact A positioning element to which it is positioned or fixed.

Another aspect is an uptake of biological material. axially mounted on the centrifuge rotor in relation to the rough positioning a cup holder deflection angle relates to a process. The process includes water stages means: a positioning element, interacts with the positioning counter element; cup holder, a stop for the rotational movement of the container holder contact part and the angle of deviation of the cup holder, of the positioning element and the stop of the positioning counter element. positioned by interaction.

The yaw angle is also known as the swivel position.

Positioning, optionally, as previously described here positioning element for angular positioning carried out through Positioning optionally cap a stop mounted on the centrifugal rotor of the holder It is performed by pressing against the part.

Another aspect of the invention is a method for retrieving biological material. relates to the container holder for the container. cup holder, a suitable for use in a centrifuge and intended for holding a container holder and a positioning element having a positioning counter element. this hang up angle of the cup holder in the centrifuge to be positioned Allows determination of position and/or angle of deviation.

Cup holder optionally additionally It contains a connection element suitable for connecting to the centrifuge.

The connector optionally defines a pivot axis.

between the positioning counter element and the cup holder center. distance cover optionally with positioning counter element is greater than the distance between the holder edge.

Positioning counter element optionally available a space for placing the positioning element contains. Positioning with the positioning counter element optional positioning of the connection between the element positioning in at least one plane direction with the counter element It is suitable in terms of preventing relative movement between the elements.

Cross section of the cavity optionally against the cavity interior decreases. The two opposite points of one side of the space the distance between the said positioning optionally from a depth at which the element can be inserted into the cavity, or is smaller than the depth of motion. Cup holder, optional It is a device for processing liquids.

Another aspect of the invention is the processing of biological material. relates to an apparatus containing water parts intended for a centrifugal; a centrifuge for removing biological material a holding member for holding the container; and a positioning element. The container carrier is passed by the positioning element. angular position and/or deviation of the cup holder in the centrifuge a positioning shaped to position its angle contains the opposite element.

Also, if the apparatus in question can be operated easily it is useful. For example, to feed the consumable and and apparatus for removing used consumable easily accessible from the front or some different It is useful to use parts. Especially this part such as in a drawer or many drawers to be provided as a removable or pull-out partition it is useful.

In addition, the apparatus in question in the initial stages of typical operation or It is also useful that it can detect installation errors. For example, user inputs with other inputs and the chosen procedure. It is useful to check the consistency. Also, consumables of loading materials with each other or at a selected also checking for consistency with the procedure (e.g. processing with liquid, containers and/or pipette tips).

User routing is as simple as possible as intuitively as possible by realizing It can also be started after the least possible input stage. it is useful. For example, using a graphical display providing a structured menu guidance it is useful. Also, that menu orientation is rarely input fields that need to be changed are normally Accessible via optional sub-Henus that can be skipped It is also beneficial to be designed in such a way that separately in question recent or frequently used settings of the apparatus It is also useful to store and provide for quick access.

In addition, the apparatus in question is flexible to many different procedures. It is also useful to be able to adapt it in some way. especially for example Broad spectrum as supplied by QIAGEN company is also useful. In addition, for processing biological material other procedures, through software updates adding or modifying existing procedures. it is useful. In addition, for the purposes of examination of the apparatus in question Ability to detect variable number of samples (batch size) and/or support them flexibly.

Adjusting the temperature of a centrifuge and especially cooling the centrifuge is also beneficial. For this purpose, biological containing water particles associated with centrifugation of material a centrifuge is provided: a chamber, a lid for the chamber, centrifuge rotor, centrifuge arranged in the chamber a drive element for the rotor, for cooling the cover the first cooling device and the second for cooling the chamber cooling device.

The first cooling device is optionally a refrigerant It is a cooling device for cooling the chamber through the

Optionally, there is an option for washing the chamber with coolant. Includes washing device. Cooling of the first cooling device gas is optionally ambient air.

The second cooling device optionally covers the outer surface of the chamber. comprising a washing device for washing with a refrigerant it does. The cooling gas of the second cooling device is optional ambient air. Optional second cooling device, control independently of the drive element of the centrifugal rotor and/or the second cooling device can also be driven It is designed to cool the element.

Optional primary and secondary cooling devices is different. Optional first and/or second cooling The device contains cooling channels. Optional word The cooling channels in question are aligned horizontally and/or vertically.

Optional first and/or second cooling device active It is a cooling device. Optionally first and/or second Each washing device of the cooling device contains a fan. it does. Optionally, the cover can preferably cover the inside of the chamber. It is suitable for closing from the outside of the chamber. Optional hopper, lid. that refrigerant will not flow into the chamber when closed or the first and/or the second cooling device into the chamber. It is designed in such a way that it cannot flow.

Another aspect of the invention is biological centrifugation in a centrifuge. relates to a process for the material. The process in question contain water stages. means: of a centrifugal rotor. biological loading with material; centrifuge using a lid closing the reservoir; a driving element of the centrifugal rotor rotating it about the rotor axis by means of; of the chamber and cooling of the cover.

Optional cooling of the centrifuge chamber, centrifuge using a washer of the outer surface of the It is carried out by the method of washing with cooling gas.

Optionally, the inner surface of the centrifuge chamber or The inside of the centrifuge chamber is not washed with refrigerant.

Optionally, the washing device is independent of the drive device.

Optionally centrifuge rotor inside the centrifuge chamber is arranged.

In addition, a centrifuge containing water particles is provided: a centrifuge chamber; arranged inside the centrifuge chamber a centrifuge rotor, a cover for the centrifuge chamber and A device for cooling the hood using ambient air.

The apparatus is located inside the centrifuge chamber for ambient air. Defines a separate flow path from

Optionally, the apparatus for cooling the cover, the cover includes a washing device for washing with air.

Optionally, the cover has an interior and the flow path, passes through the inside of the cover. Optional cooler channels, a cover in thermal contact with the flow path aligned in the region. Optional cooling channels, flow aligned in the downstream direction of the path. Optional cover, contain means for removing heat from the interior of the centrifuge. it does. Optionally. environment. atmosphere, environment. is at the temperature.

Optionally, the apparatus for cooling the cover, the absorbed medium the velocity profile of the air, preferably cut at an angle and different using refrigerant duct edges retracted at angles Includes tools for balancing.

Described for the processing of biological material to cool one of the centrifuges, especially the centrifuge a device equipped to control the temperature. An apparatus comprising a centrifuge is also provided.

Another aspect is with a process for cooling a centrifuge. is relevant. A centrifuge chamber in the centrifuge, centrifuge a rotating centrifugal rotor arranged inside the chamber and a cover for the centrifuge chamber. Aforementioned The process includes water stages: removing ambient air and the flow of ambient air leaving the inside of the centrifuge chamber Passing along the cover along the path. Optional word The process in question was carried out in one of the described centrifuges, i.e. in a centrifuge equipped to control the temperature is performed.

Find out. another' aspect, biological centrifuge in a centrifuge by a process involving the water stages of the material relates to: loading the centrifuge with biological material; the rotator wheel in the centrifuge chamber inside the centrifuge, by means of a drive element that rotates about the rotor axis. rotate; of the drive element and the centrifuge chamber washing the surface with a coolant.

Independent of the described arrangement, a centrifuge Other aspects are also presented. In particular, biological material contains the following components for centrifugation A centrifuge is provided: at least one of the biological material a rotor that can rotate about its rotor axis to receive a container. steering wheel; centrifuge chamber and rotor axis between the rotating rotor wheel and the centrifuge chamber. a centrifuge containing a shield placed is presented, the height of the outer border of the rotor wheel, the cabinet or container holder during the centrifugation process. higher than the height of the position of its upper edge. are below.

Another aspect is the surface height of the substance in a container. relates to a process of determination. The article in question optionally liquid but may be mixed with another, such as a powder It could also be the item. The process in question includes the following stages: the surface of matter is irradiated by a radiation source; matter radiation from its surface is measured with a radiometer; radiation based on radiation measured with a radiometer The source, the radiometer and the container are in a spatial relationship with each other. so that radiation is perpendicular to the matter surface. The first angle between the first beam passing through its source is essentially with the first beam from the material source to the radiometer second between the reflected beam and the direction perpendicular to the material source equals the angle; and the height of the matter surface of the source, the radiometer, and the cabin spatial relationship. determined as a function.

Optionally, irradiation between 0.3 mm and 3 cm, preferably between 1 mm and 6 mm, eg 3 mm pen diameter carried out with a pen beam. Optional word The surface in question is an interface between air and liquid. Optional Additionally, the radiation source and the radiometer are in the same housing.

Optionally, a radiation source and a radiometer light in the sensor. Optional radiation source, The radiometer and the cabinet are in a spatial relationship with each other. detection involves bending the radiation source. Optional curvature of the liquid surface caused by adhesion forces may be notable. Optional material surface height, the case where the first and second angles are the same determined using.

Another aspect is a process for processing biological material. relates to the apparatus. The apparatus in question is capable of receiving a substance. oriented a container holder and substance in a container a fill level for determining the height of the surface includes the meter. In addition to the fill level meter, the substance a radiation source for heating its surface; matter a irradiator for measuring the radiation coming from the surface sensor; a positioning device and a control unit contains. control unit, radiation source, the radiometer and the container, a measure of the radiation measured by the radiometer will be spatially related to each other as a function of so that they are perpendicular to the material surface. first between the first beam passing through the radiation source The angle is essentially the first beam going from the material source to the radiometer. between the reflected beam and the direction perpendicular to the material source. equals the second pain; and the height of the material surface radiation source, radiometer and cabin spatial determined as a function of the relationship.

Optionally, the optical sensor of the level meter or positioning a sensor unit with a light sensor moving or the presence of a cabinet as described in this application. It may also be appropriate to determine

In addition, the apparatus in question is used for biological material processing. is the apparatus. The apparatus consists of an optical sensor and an ultrasound. a movable unit to which its sensor is attached; of the optical sensor to the liquid reservoir as a function of the sensor data. the presence or absence of a liquid reservoir in a holder for detection tools and the sensor of the ultrasound sensor contained in a liquid reservoir as a function of Contains tools for determining the level of liquid Regardless of the arrangement described, the Other aspects of the sensing device are also presented. Therefore, containing water particles for biological material processing An apparatus is presented: an apparatus for receiving biological material. holder for container, at least one container with increased recognizability container containing the partial; for heating a recording area in the holder connected to the container piece with increasing recognizability radiation field; the radiation intensity from the recording area. a sensor for measuring; measured density, defined equal the presence or absence of the vessel by making a comparison between an evaluation unit equipped to record.

The vessel optionally includes a centrifuge vessel, a filter vessel, a collector cup or pipette tip. For example, as it is known Centrifuge vessel such as spin tube or Eppendorf tube and the collecting container is usually the container or container of the material main opening for pipetting and optionally a Includes cover. In the centrifuge chamber, there is usually also a main on one side of the centrifuge cabinet opposite the opening There is a regulated exit opening. in increasing recognition The container part optionally consists of a membrane, a filter, a filter. membrane is a lid hinge and/or container lid.

The sensor in question is optional. is mobile. Aforementioned this way, the apparatus can be heated by an adjustment mark on the apart. and sensor position by measuring a radiation from the gauge light may be suitable for identification. In this way, the sensor movement optionally in at least one direction, preferably in both directions container for tip picking or pipetting unit it depends on a gripper movement.

As will be explained below, the sensor in question is optionally available. presence of a substance or level of filling in the container can determine. Radiation from the holding zone optional as the radiation source. reflected radiation. Promise The radiation in question is typically electromagnetic radiation and preferably in the visible or infrared spectrum range.

Evaluation unit optionally measured under threshold absence of density and density measured on equal It is designed to record its existence.

Optional irradiation, increased recognizability vessel a spot with a diameter between 0.3 times and 1.3 times the carried out using Radiation source and sensor can optionally move together. In this case the word The apparatus in question additionally covers the radiation source and the holding area. for positioning the connected sensor. positioning tools contains.

Optional in radiation from the radiation source It has a spot diameter of about 3 nmi. with increasing recognition optionally at least 3 mm x 3 mm area in the container part has. The pot portion with increasing recognizability generally increasing signal difference or density between presence and absence of vessel It is a container part that shows the difference.

Another aspect is in a holder for said container. the presence of a container for receiving biological material, or It relates to a process for detecting its absence. Promise The process in question includes water stages: the residual in the holder The recording area associated with the recognizable cabinet door part is illuminated; radiation intensity from the recording area measured; the presence or absence of a vessel is measured by density recorded by comparison between a defined threshold value.

Irradiation and measurement optionally with a movable sensor is performed by the unit and the process is optionally In addition to the above, the water stage contains: the container positioning of the sensor unit attached to its holder.

Optionally, the location of the sensor unit on the apparatus by illuminating the calibration mark and determined by measuring the radiation.

Another designed direction is for processing biological material. relates to an apparatus and includes the following parts: container holder for receiving a container for biological material; a sensor with an ultrasound source and an ultrasound sensor unit; of the sensor data from the ultrasound sensor as a function of determining the state of the vessel opening and as a function of the sensor' data of the ultrasound sensor when the cabinet is open, the possible presence of the substance in the container an evaluation unit to determine

The substance is optionally a liquid. evaluation unit optionally possibly the ultrasound sensor fill level of the substance as a function of be determined or regulated accordingly. Evaluation substance with optional emitted ultrasound signal reflected by its surface. between an ultrasound signal suitable to determine the working time by measuring or is designed for. Sensor unit optionally movable and additionally contains water parts: a radiation sensor and a function of the sensor data of a radiation sensor to position the sensor unit connected to the container as oriented positioning tools. Radiation sensor optional As a result, it is suitable to determine the presence of the container. determination, for example any of this reference to determining the existence of a container using the methods described in can be performed (eg next page).

In addition, at least one container of increasing recognizability in said container register part of the sensor unit in the holder there is a radiation source to illuminate the area, of the recording area is associated with the container part with increasing recognizability. It is claimed to be. In this case, the radiation sensor It is suitable for measuring the intensity of the radiation coming from the field, the density measured in the evaluation unit. and defined threshold the presence or absence of the vessel by making a comparison between equipped to record.

With an apparatus for processing biological material. related water another aspect is also envisaged, which has parts: a container container station for maintaining its feed; cap station containing information on the nature of the containers. sign; and a radiation sensor for reading the sign.

The beacon can optionally come from a corner of the hopper station. It is a protruding element. Hopper optionally at least one signal element integrated with the station contains. Signal optionally at least two sign elements Each of these contains yes/no information. encodes binary information. Hopper station optional can be provided with various directions regarding the apparatus. or it can be inserted into the apparatus. is configured as follows.

Sign optional, various of sign reading independent of a special referral selected from referrals It is configured as it is.

Hopper optionally includes multiple holding positions each of which is for a container and the optical sensor is one cabin in each holding position of the plurality of holding positions suitable for detecting its presence. Described in the paragraphs below The method used can be used for this detection process.

Optionally the chamber station includes a radiation source. it does. The radiation source in question is, as of this section, for one or more functions that describe available: lighting a recording area; sign illumination of the element. Optional hopper station additionally multiple holding positions, each for a container containing at least one of increasing recognizability. comprising the container portion; radiation source covers a recording field suitable for lighting, and the recording area has increased recognizability. associated with the special container part. Radiation sensor optional as a measure of the intensity of the radiation coming from the recording area. is suitable, the evaluation unit is measured density and defined by making a comparison between the threshold, the existence of the container or equipped to record its absence.

The cups are optional pipette tips. The cabin in question quality of the pipette tip, pipette tip material, or may be the collection volume of a material contained in the pipette tip.

The optical sensor is optionally a light sensor. This sensor optionally with the radiation source is a part. The said radiation source is optional. suitable for illuminating the sign element of said sign and said apparatus, reading the sign; signal through the sensor measure the intensity of the radiation coming from the element and radiation to compare the intensity with a specified threshold. can illuminate the pointer element through its source.

Another direction is at the hopper station for said container. determining the nature of the container for the collection of biological material relates to a process for The process water contains the steps: containing information about the cabin quality the first sign on the hopper station is illuminated; first the radiation intensity from the beacon is measured; cup type, measured recorded as a function of the first density. Optional chamber containing information about the cabin quality. the second sign at the station is illuminated; from the second mark the second incoming radiation intensity is measured; container nature, as a function of the first and second density measured saved.

Another aspect is the use of a centrifuge. relates to the process, each of which is a biological material a plurality of container holding positions for the container. process, contains water stages. For container holding positions it is recorded whether a cabin is available; cap holding Total number of containers currently saved at their location is stored; in the container holding positions. saved as present based on the total number of containers at least one condition for distribution in holding positions determines; recorded as present in the door handle positions an examination of the conformity of the distribution of the containers with the relevant condition. control is performed. Sample container position optional in a shaker and/or heater for the sample cabinet is arranged.

Other aspects are that a centrifuge with multiple cup holders relates to a process for controlling the occupancy.

Each of the container holders has at least one handle for biological material. relates to at least one container holding position relative to the container. Each for the cup holder in the respective cup holding position an empty state is defined where no cabinet is available, and at least one cabinet is present in any respective container holding position A full situation is defined. The process water includes stages: word for pot holders whether the cup holders in question are empty or full saved; distribution and total number of fully loaded cup holders saved; full container based on total number recorded at least one condition for proper distribution of holders determined and the number of full container holders recorded a statement regarding the distribution of control is done. Optional in cup holding positions The total number of containers available is also recorded.

In these processes, the cup holding positions are optionally centrifugal. It can be rotated with its rotor.

In these processes, a sensor is optionally a cabinet. records whether the container is in the holding position and the process additionally determines the number of sample vessels via the sensor. This includes the step of registering the sample container locations.

Processes optionally also include the number of sample containers, correlated with the total number of containers currently recorded incompatible, that is, whether the two numbers meet each other. or the number of sample cups, cup holding Check if the set of positions matches the number of includes the act of doing.

Processes also optionally include a container holding position creating an association that assigns it to the sample cabinet includes the stage. In this case, that association Optionally, each sample cabin is available in the form of a cabin. assigns a container hold location where it is saved, or a group of containers. It assigns a sample container to the holding position. a the cabinet is present in at least one container holding position of said group is recorded as. The association in question is typical Alternatively, the sample cabinet is a sample chamber connected to the container holding position. with the regulation of the instructions for repositioning the container is created. The sample chamber position is optionally a common the container in question is allocated to the holding position by naming.

The sample cup position is optionally available with a sample cup. the layout of the sample container locations at the corresponds to the arrangement of cup holding positions in the centrifuge is allocated to the container holding position based on its status.

Associating, optionally the respective container holding position, a table that assigns the sample chamber position to the specimen chamber is created and created.

Another aspect of the invention is a method for processing samples. with a process for checking the fullness of the apparatus is relevant. The process includes water stages: with samples the number of sample containers to be prepared are recorded; samples based on the number of sample containers prepared. The number and/or amount of consumables required for processing determines; number of consumables in said apparatus and/or ink amount, with the number of consumables required and/or compared to the amount.

Optional cup holding positions, with centrifugal rotor returns together. Optionally, the required consumables The number and/or amount is determined in accordance with the relevant protocol.

Optional consumables, containers, especially straws ends, filter cups, for example spin tubes and containing collection containers such as Eppendorf tubes. selected from the list. Optional consumable biological substances, eg biological liquids, enzymes and/or buffer fluids. Optional consumable from the determination of the actual status of the materials to the user. references to produce the desired state is created.

Another aspect is a method for holding multiple cup holders. a rotor or a device that contains devices for handling liquids. It's about centrifugation. Each of the container holders, centrifugation, i.e. rotation of the rotor around the rotor axis It can hold the first container during. The centrifuge additionally a processing unit that can remove the container from the respective container holder contains. Especially when the cup holder is in the centrifuge. realizable. In addition, the processing unit first container, may bind to the second container, or at least partially can be inserted, the second cup is held in the cup holder.

Optional gripper, movement in x and y directions can be moved, x KK: with pipette units in y directions can perform a movement and/or move in the z direction.

Movement in the Z direction is generally independent of the pipette unit.

The gripper can be rotated and the containers re-inserted in the container holder. can position. This reposition is usually involves rotating the gripper.

Another aspect of the invention is biological in a centrifuge unit. It relates to a process related to the processing material. Centrifugal unit has a rotatable centrifugal rotor, centrifuge a container holder and biological material arranged in its rotor the first holding position in which a cabinet is held for receiving and a the gripper unit comprising the gripper. Aforementioned process, water steps to remove the container from the first position includes: to provide access to the first holding position positioning the gripper; cabin a gripper from the cup holder with the cabinet gripper moving the gripper to remove it.

The process in question is optionally a rotary position. determination or rotational movement of the centrifuge rotor. includes an additional step to prevent Optional the cup holder is mounted axially on the centrifuge rotor. and said process, the cup holder in the centrifuge rotor includes the step of preventing the rotational movement.

Description of Independent Aspects Whole System Parts Overview Figure la, work in accordance with the present invention shows the top view of the platform (2). Biological procedure steps for the processing of substances A few useful modules to implement is shown. Figure la shows the following modules: for centrifugation of biological material centrifuge unit 200 (see Figures 3a to 5); a waste disposal, such as used pipette tips station 740; relevant stages of the procedure for biological substances Regarding various consumables consumables station 700 (see Fig. 12); aforementioned consumables station, among other things buffer liquid for performing the procedural steps a hopper station (710) for holding hoppers found contains. Consumables for consumables pipette station (700) additionally from a pipette unit a pipette tip station (720) for tip removal and other procedures to carry out the steps contain other reservoirs for liquids. to heat biological material and/or other liquids and/or a heater/shaker 600 for agitation (see Fig.

Figures 13 and 14). Heater/shaker e.g. lysine stages can be used during execution. input and output of user routing and other information A screen (910) used for

Individual elements shown, modules as much as possible edited as. These are added to the work platform individually. can be assembled and disassembled and preferably placed is in the structure. For example, power supply or individual modules additional connections for the control of the device via plug connections makes. Individual modules can be easily exchanged working platform of the modules in question transverse shown on the work platform below corresponding substantially to their cross-section and thus vertical It is preferred that they cover an area where they can be pushed as

However, due to space, the individual cross section of the modules shown on the work platform It may also be advantageous to cover less area than the cross-section. The modular nature of the work platform means that the individual modules alternative regulations that vary in the regulation specifies. In this section, special space requirements are taken into account. receivable. In addition, as shown in Figure la, the individual Alternative arrangements are possible in which modules are omitted.

Therefore, for example, to realize the lysis stages, the heater/shaker (600) negligence unless it is necessary can be done. Similarly, other process steps realization or previously supported process steps. alternative modules for better realization can be added. Other module examples include: a UV lamp; active cooling for the heater/shaker; eg cap holders, containers, buffer fluid and other. processing civilians for automatic loading of consumables such as apparatus; analysis modules for analyzing the processed liquid, an apparatus for disposing of used consumables; And other modules.

Of an apparatus for processing biological material Another top view of the work platform. Shown in lc. In contrast to Figure la, Figure lc Centrifuge cover of centrifuge (200) in top view shown (240) is omitted for illustration purposes. This is the centrifuge. rotor 212 and a container holder 120 arranged therein. means 'visible'. income. In addition, the centrifuge cover air inlet (253) and air outlet for cooling system channel (255) is visible. In addition, the centrifuge cover a mechanism (260) for opening and closing and a centrifuge an element (268) for fixing the cover in the closed position is visible. These elements, with reference to Figures 3b and 5 is explained in more detail.

Additionally, a gripper unit (400), a straw unit (500) and a gripper for moving the pipette unit. the transport system 300 is shown.

The transport system (300) (also Figure 6), the apparatus in question, For example, an X fixed to the rear wall of the apparatus. rail (312) (ie its direction is the X direction of a coordinate system) and a Y rail (314). of the Y rail one end of said Y rail in the X direction of the X rail (312) that you can move the sliding element (318) It is mounted on the X-rail with said sliding element (318) in the figure.

The Y-rail is held horizontally by the slider. This Although the situation is not shown in the drawing, alternatively, the operation Y that can slide along the second X-ray attached to the platform (2) providing a second sliding element at the other end of the rail, and thus with a sliding element at both ends of the Y-rail. possible to hold.

Carrier member 320 moves along the Y-rail in the Y direction. can be moved, this carrier element (320) supports the gripper unit. (400) and pipetting unit (500). gripper unit and pipette unit each independently in 2 directions The carrier element 320 can be moved along (ie. vertically, ie perpendicular to the drawing plane in Figure lc). This the gripper unit (400) and pipetting unit (500) each It can move in direction 2 and the movement in direction 2 is independent.

Depending on the complexity of the procedure followed and the layout of the modules to move the gripper and pipette unit based on Alternative arrangements are also possible. For example, that the gripper unit (400) and the pipette unit (500) can be moved in one direction (eg in the z direction) or in two can be moved in the x direction and in the 2 direction an arrangement is possible. In this case, for example, the Y rail (314) is neglected. and the carrier element 320 is attached directly to a fixed rail. can be attached. In addition, either or both rails can be replaced by, for example, one or more elements and so, for example, a pivot to which the support member 320 is attached. arcuate motion can be produced through the arm. Moreover desired procedure of gripper or pipette unit promise if it is not necessary to carry out the steps of units can be neglected.

Slot, Cover Said apparatus is arranged in a housing (4). Aforementioned The apparatus further includes a cover at the front of the housing.

The closed state of the cover is shown in Figure 2a. Slot (4) e.g. metal, plastic or other resistant material may be the product. On the cover (10), a handle for manual opening (12) are available. The cover (10), the working of the inner part of the apparatus transparent so that it can be viewed optically during is in the structure.

The cover (10) and the housing (4) are soundproofed. it is advantageous. For this purpose, the housing (4) is provided with a sound-absorbing inner lining. can be done. Vibrating parts in the housing also sound insulation provided, it can be flexibly swayed. Also on the cover A sealing member for (10) may be provided.

Additionally, Figure 2a illustrates the screen 910 in question.

Drawer of the waste bin (750) for consumables element is also shown. Drawer element a handle (752) can be pulled from the front of the apparatus through In addition, a speaker hole (902) for loudspeaker and on/off button (6) is shown. Using the waste box, this It is explained in more detail in the next section.

Figure 2b shows the apparatus in Figure 2a with the cover (10) removed. shows. As a result, on the inside of the slot (4), Figure 1a and the centrifuge cover (240), shown at lc heater/shaker (600), fork-type light barrier (852), work platform with hopper station (710) and Y-rail (314) (2) is visible. In addition, for holding the cover (10) holders ~ (14) are shown. Holders (14) up can rotate around the axis so that the cover can be manually can be opened upwards.

centrifuge and other components, stability will be achieved and with vibration, the influence of neighboring nmdullars will be greatly suppressed It is integrated into the apparatus platform in this way.

Centrifugal Figure 3a, perspective view of centrifuge 200 shows. In this view, the centrifuge cover (240) is closed. centrifuge rotor (212) cannot be seen. Centrifugal cover 240 includes a cover cage 244. Cover (240) other parts are described in reference to Figure 3c. Shape A centrifuge chamber surrounding the centrifuge rotor at 3a (220) is shown. The centrifuge chamber in question, aerodynamically separates the centrifugal rotor from the environment, thus reduces noise and parts parts at high rotor speed It provides a safety precaution in case of separation from each other.

In addition, centrifuge in the form of asynchronous electric motor drive member 218 is visible. For example, the drive element Other forms using a belt or chain are also possible.

Drive element shown approx. 0 - 15,000 rpm designed for alignment.

In Figure 3b, the centrifuge (200) in question is the centrifuge cover (240) is displayed in the open. Here is a centrifugal axis (214) a rotating centrifugal rotor (212) is shown. Also around the centrifugal axis (214) a shield that can rotate with the centrifugal rotor 212, or rotor bowl 216 is shown. Centrifugal rotor (212), means for suspending a container holder (120).

One such container holder 120 is shown in Figure 11 .

In principle, the various positions provided for this purpose are may be filled with its holder, preferably any unbalance centrifugal rotor of cup holders to minimize Pay attention to ensure that it is distributed homogeneously around should be done. In addition, non-rotating with the centrifugal rotor 212 The inner wall 222 of the centrifuge chamber is shown.

For opening and closing the centrifuge cover (240a) driven by a drive element (not shown) a transfer rod 262 is shown. Separate related pass the cover that can be snapped onto the part (268) and closed is closed. a locking mechanism that allows it to be held securely in position a closing member (267) held by (269) is shown. For the centrifuge cover, the aforementioned centrifuge chamber when the centrifuge cover (240) is closed. (220) at least partially sealing the interior a gasket (228) is also shown. Biological samples While processing, the temperature in the centrifuge is at a predetermined Better results can be achieved if kept within the temperature range. can be done. Friction generated during centrifugation minimizing the heat or evacuating outside in particular, such results curable.

The centrifuge shown in figures 3a, 3b and 3c is partially in question. It is equipped with a cooling system that meets the requirements.

The cooling system shown is the evacuation of the heat to the outside of the centrifuge. allows for. The cooling system shown is also to properly affect the air flow inside the centrifuge. allows.

An element of the centrifugal cooling system is the centrifuge chamber. 220 is external cooler 230. External cooler (230) Figure It is shown in 3a. External cooler, centrifugal heat from the chamber (220) or its outer wall to the ambient air. Cooling channels that allow it to be evacuated (232). Except for the cooling ducts (232) shown a form of surface cooling may also be considered. For example, another Configured cooling ducts, especially heat-conducting material coating or the heat effectively into the ambient air Other surfaces are also possible.

Above 50 W/(m2 K) regardless of the arrangement shown a surface or arrangement with a heat transfer coefficient heat transfer coefficient over 100 W/(m2 K) It is especially preferred and has a heat transfer rate of over 150 W/(m2 K). coefficient is most preferred.

In addition, the shown fan (234) 220 performs the external cooling function. air, blows on the outer wall of the centrifuge chamber. This is also results in more efficient evacuation of heat to the outside air. However, said ventilator (234) may additionally be omitted or with an alternative refrigerant such as nitrogen or a liquid A device for washing with a refrigerant (eg water cooling) interchangeable with other devices such as

Lower the centrifuge (204) to direct the airflow outward. There are holes (205) in the part. On one side of the apparatus (1) suitable air such as the arrangement of the centrifuge or hoses If the outlets are provided, the lateral air removal slots It is also possible to use. Mention in the regulations shown the mentioned fan (234), cooling channels (232) or the outside of the chamber wall (222) as well as the centrifuge rotor (212) drive member (218) with a refrigerant. It provides the advantage of washing.

The centrifugal cooler shown in Figures 3a to c consists of air or centrifuge chamber of any other coolant prevents it from entering. In addition, gaskets (228, 229) and the nature of the protection ring (215a) and the chamber wall (222), cooling It helps to prevent the leakage of gas. shown regardless of the regulation, the refrigerant gas, centrifugal If it does not overflow the inside of the reservoir (220), this The situation is advantageous. In other words, cooling air or another refrigerant does not enter the centrifuge chamber, so the inside of the centrifuge chamber is made of refrigerant or protected from gas current.

Centrifuge cooling or from the inside of the centrifuge. Another device for heat evacuation is the centrifuge. a cover cooling for cooling the cover 240 system (250). Cooling the centrifuge cover (240), rising of the heated air in the centrifuge and towards the cover It provides benefits related to the tendency to move. Conclusion As a result, the heat can be removed from the cover particularly effectively.

Various cooling devices (250) for the centrifuge cover (240) regulations can be considered.

A similar arrangement is shown in Figures 3a to 3c. Promise This arrangement is designed to cool the centrifuge cover (240). that takes ambient air and blows it against the centrifuge cover (240) a ventilator 251. air, an air intake it is directed from the channel (252) to the centrifuge cover (240). Weather, an air inlet (253) and the corresponding air inlet towards the cover (240) via an input device containing aperture 258) taken into the centrifuge cover. The air is inside the centrifuge cover (240). centrifuge cover by passing through or blowing absorbs the air and carries it out again. In this way, the air is centrifuged The heat can be evacuated from the cover. air, an air outlet evacuated through opening 254; heat passed through the cover air passes through the air outlet opening (254) through the cover. can escape, through the air outlet duct (255) to the base of the apparatus. it is directed out of the apparatus.

Figure 3c, centrifuge with lid cage (244) removed cover 240 (see Figures 3a and 3b). This of the centrifuge cover base (242), air flow cooling ducts (256) that define the ducts (257) associated with It shows what you have. Cooling ducts (256) and cover expanding surface, centrifuge cover (240), cover. flowing guarantees adequate heat dissipation to the air. In the chamber (232) similar to the cooling ducts, here in the cover alternative surface forms are likely to exist.

Alternative that differs in terms of cover cooling quality expressions are also possible. For example, the cage of the centrifuge cover (244) can be removed and/or ventilator 251 can be omitted.

In the second case, the centrifuge cover is passively supplied with ambient air. can be cooled. In addition, the lid may contain a refrigerant such as water. Liquid cooling is also possible. In this case, flexible tubes Using the valve, the cap does not have any difficulty despite the tubes. of the coolant in such a way that it can be turned on and off without feeding to the lid surface and taking it from the lid surface it is useful. 50 W/(m2 K) regardless of the arrangement shown a surface area with a heat transfer coefficient above While the arrangement is preferred, a voltage above 100 W/(m2 K) coefficient is particularly preferred and above 150 W/(m2 K) coefficient is most preferred.

Figure 3b shows the rotor bowl as another component of the centrifuge. or the shield (216). Connected in Figure 4 As explained more accurately, the rotor bowl (216) heat development and affects the airflow inside the centrifuge chamber (see Fig. 4). Regardless of the arrangement shown, in the rotor bowl (216) any holes or holes off the centrifugal axis (214). no further clarification has been provided.

Additionally, the centrifugal rotor 212 is shown in Figure 3b.

12 intake positions on rotor for receiving cup holders 120 available. Receive locations are numbered. with the number "1" The first pickup position marked is optionally also easily discernible as the colored mark (not shown) may be marked with an acceptable sign. Rotor to cup holder Includes holders for suspension means (110) for (120) it does. Suspension means (110) for the container holder (120) suspended from the holders, they are horizontally attached to the rotor (212). can rotate on one axis. Figure 3b, for example with suspension tools (110) suspended on the rotor (212) and around the horizontal pivot axis shows an outward rotating cup holder 120 . Hanger tools (110) integrally with cup holder (120) can be created or created separately from it. Second In this case, the cup holder (120) is attached to the suspension means (110). can be designed to be placed. a preferred According to the regulation, the cup holder is made of plastic and the hanger tools are made of metal such as aluminum.

The rotor is also attached to the container holder (120) or the suspension means. (110) to the maximum pivot position around the horizontal pivot axis Represents a stop piece for stop piece, the car defines the maximum pivot angle, where When the maximum rotation angle is achieved, the rotor (212) part contacts. Preferably. maximum pivot angle, external axis direction, that is, the rotor (212) rotates around its axis (214). rotation produced by centrifugal force is in the direction.

Different suspension means (110) or, for example, the cup holder. (120) on the suspension means (110) different orientations as well as the container for mounting the holder (120) on suspension vehicles (110). different maximum pivot with the use of different methods Identification of different stop parts for angles supportable.

In addition, it is necessary to rotate the cup holder 120 on the inside. A stop can be provided for Both stop part of the centrifuge rotor (212) or another part of the centrifuge. may be mounted on some of its parts (200). to the stop and Patent Application No., Page 39, Line 11 to Page 43, Line 6 spacing, Figures 17 and 18, and Claims 61 to 68 is defined. Figure 4 is a diagram containing rotor axis 214. lateral cross-sectional plane passing through the centrifugal rotor shows the cross section. Output parts of motor shaft (215), a rotor wheel (212), a cup holder (120) and a rotor The bowl (216) is mounted rotatably about the rotor axis (214). is done. The motor (218) rotates itself around the rotor axis. moves. The rotor axis shown in the figure and in question there are non-rotating elements around the chamber wall (222) of the centrifuge chamber (220), the chamber a hopper carrier (226) for supporting the wall of the engine Connect the fixed parts (218) to the outer wall of the engine (218) and the hopper. a sealing element (229) between the wall (222) and the space between the chamber wall (222) and the centrifuge cover. a sealing ring (228) to seal (not shown in Figure 4). In addition, the engine The protection ring (215a) of the shaft (215) outlet is located inside the engine (218). part of the centrifuge chamber (220) and the inside of the centrifuge chamber (220). acts as a separation.

Output of motor shaft (215), rotor wheel (212) and rotor bowl (216) are interconnected. Rotor wheel (212) user can be changed by For this purpose, the impeller motor through the screw element of the output shaft (215) of the motor shaft. it is screwed to the outlet (top element of the motor shaft 215 outlet) and motor with additionally a pin for transmitting rotary motion it is connected to the safti outlet. Rotor for mounting the pin A recess 212a is provided in wheel 212. Indent (212a) to ensure that it does not cause any imbalance A sync hole (212b) is also provided.

A cup holder (120) in the cross-sectional view of Figure 4 is shown. The container holder in question is further illustrated in Figure 10. shown in detail. Cup holder, eg hanger It is placed on suspension tools (110) such as

As shown in Figure 5, the suspension means 11O, centrifuge It can be hung on the rotor (212). Relevant suspension tools for this purpose (110) is the suspension of the centrifuge rotor (212) provided for the purpose. to the elements (112b) in question. to place the suspension tools appropriate axial portions 112a. hanger tools When the (110) is hung on the centrifuge rotor (212), the tools and placed cup holder (120) horizontal axis (113) can rotate around it.

Figure 4 holding the container holder attached to the horizontal pivot axis shows in position. occur during centrifugation. incoming centrifugal forces push out of the cup holder causes it to rotate on the pivot i.e. the bottom of the cup holder, away from the centrifuge axis (214). Do not turn outward to limit its movement, as shown in Figure 5. stop piece is provided. Stop piece, in Figure 5c centrifuge when maximum pivot aS is achieved as shown of the suspension vehicles contacting the passing edge (ll4b) of the rotor It is formed by one edge (114). The other part of the stop with details of patent EP-A-1767274, Page 42, Line 7 It is described on page 43, Line 6. cup holder, cannot turn inwards due to the stop arrow. This move of the stop (212m) (see Fig. 4) and/or the centrifugal rotor (212) stopper (116b) in Figures 4 and 5, respectively. the corresponding engagement of the suspension means (110) as shown. based on contact with the stopper (110 m or 116a) is blocked.

During centrifugation of biological material, the container the containers for biological material in the holder (120) It is not necessary to have closed covers. Actually, they may not have any cover or the cover is open it could be. For example, the containers shown in Figure 4 the open lids held in the lid chamber of the carrier (120). may have.

In the centrifuge bowl 216 shown in Figures 3b and 4 , there is a lower region extending towards the centrifuge axis (214).

There is also an upwardly curved outer edge region.

This arc, the cup holder, is motionless in the centrifuge rotor (212). position, the outer edge of the centrifuge bowl (216) between the lower edge and the upper edge of the said container holder (120) It means that it is at a height that is found.

The centrifuge bowl 216 also contains an interior, ie roughly extending to the height of the outer rim of the bowl. a `region defines. At the same time, the outer and lower parts of the centrifuge bowl (216) its part, ie the edge (220) towards the centrifuge chamber, defines a relatively smooth surface and thus turns Air friction can be minimized during However centrifuge bowl (216), top of centrifuge chamber (220) as it does not extend to the edge of the centrifuge chamber, centrifuge by draining towards the wall (222) air exchange on the wall of the chamber 220 realizable. Thus, more effective cooling is obtained.

Figure 10 is a container holder for holding a cabinet 160 (120) (see Figure 11b). the container in question three holding positions (130, 140, 150) for containers available. Each of the holding positions is in the same container or in another can be provided for containers. For example, shown in Figure 10 on the cup holder than the corresponding holding positions 130 and 150 holding position for a large diameter container (140) has been provided.

The cup holder also has a volume 122 capable of holding liquid. It may or may not contain a clear Again The holding positions 130 and 140, with reference to Fig. 11a, emerging from a cabinet lower opening in the holding position (130). it includes an opening through which the liquid can pass into the volume 122.

However, in the holding position 150, the liquid leaving the held containers, cannot enter volume (122) because these grip positions do not leak solids. surrounded by a wall. Hence the holding position 150 can act like a container.

As shown in Figures 11a to d, the grip position 140 The diameter (A2) of the cup holder (120) is the position of the holding positions (130 and 150) diameter (A3=Al). As a result, the first (170), an outer anchor (A2) that allows the container to be placed, and inner diameter corresponding to the diameter of the other two holding positions (A3:A1) it is possible to place it in the holding position 140.

Therefore, the holding positions 130 and 150 are provided. The container 160 may be placed in this container 170. In particular, said arrangement cabin 160 is removed from the holding position. 130, e.g. container (170) held in the respective holding position (140) allows its transmission through a gripper such as the gripper (400). gives. Similar methods for the transfer of containers EP-A- 8, and the said parts are available included in the description of the application. The methods in question associated gripper 410 (see Figure 9) or a similar can be applied using the gripper.

Lid on containers to be held by the container holder (120) can be found. Container (166) with lid (166) for example in Figure 9 is shown. container as shown in Figure 10. cap chambers for caps in holder (120) (132, 142, 144) are available. Lid chambers, centrifugation for fixing the cover in a safe position during is configured. The lid chambers are located in the lid carrier (414). Configured to allow access to cover (166) (see Figure 9). For this purpose, the lid chambers are (414) will act as a stop by contacting the cover. contains openings 133, 143, 145, hence the lid removing the lid from the reservoir (120) or removing the lid The relevant cover is removed from the cover chamber during its placement. removable or by cover carrier (414). the defined location can be placed at the corresponding location when retrieving.

As shown in Figure 11d for the holding position 140 two cabins (160, 170) placed together two cover chambers (142, 144) provided for lids available.

The cup holder also stabilizes the holding positions 130, 140. to the support rods (136, 146) that fulfill the function of has. For additional stabilization this retention their positions are attached to the outer wall of the cup holder (120).

Hence the holding positions during centrifugation. occur It can be kept stable against the incoming forces.

A positioning opening or positioning in the container holder counter element (124) is available. Positioning counter element (124) of the positioning device as shown in Figure 8 (450) to locating tip or locator (452) It has a shape to pass. explained with reference to Figure 8. said insert can fix an angular position or can position. Also, the deflection of the cup handle in the centrifuge can position or fix the angle. Positioning counter element (124) to receive said positioning element. contains a space for

Positioning element with positioning counter element (124) (450) with the positioning counter element (124) positioning in a plane direction, that is, in the horizontal plane in terms of preventing relative movement between the element (450) suitable. Cup holder center with positioning counter element distance between positioning counter element and cup holder is greater than the distance between the edges. of the cup holder pivot as its center is generally shown in Figure 5c It is defined by its axis (113). The gap tapers down so cross section of the cavity in a direction towards the cavity interior decreases. The two opposite points of one side of the space to the space of the positioning element in question. from a depth or course depth where it can be placed is smaller. described in the patent. All kinds. rectify the imbalance. either centrifuge as much as possible to minimize balanced loading is beneficial. For this purpose it is centrifuge, the centrifuge is as stable as possible It contains tools that enable it to be loaded. Tools, centrifuge The rotor (212) is largely uniform, even when it is not fully loaded. Contains Rules (Rules) that contribute to the installation.

According to one aspect of the present invention, the Rules stipulate that the centrifugal rotor (212) can be used for automatic loading. find it Rules according to an alternative aspect, control manual installation can be used to. In this case, the installation must comply with the Rules. unsuitable or sufficiently advantageous for imbalance warning or error message if the distribution does not have can be published. Also about the optimized loading recommendations can also be shown.

The rules are eg the weight of the containers in which the centrifuge is filled. its center is consistent with the rotor axis (214). may require. For this purpose, each vessel, direction, cabin centrifuge the angle around the rotor axis (214) on which the rotor is arranged. may be related to a corresponding Vector- In this case Rules, Vector sum zero or specified threshold may require below. Doors in different weights Vector length proportional to container weights it could be.

Other Rules may be provided for automatic installation. For example, P positions B with cup holders or with cups Rules for automatic loading, uniform distribution may require. Precisely every (P/B)th of the centrifugal rotor position is occupied if P is divisible by B. If P is not divisible by B and B' is the next greater divisor of P compared to B (B'-B) empty containers or container holders can be loaded and thus B' containers are evenly distributed.

In addition, the drive element control, the centrifugal drive element Based on data from the acceleration sensor that is part of (218) It may allow the determination of the imbalance as a result. In this case a certain amount of imbalance or the average duration of the imbalance. If the threshold is exceeded, an error message may be generated and centrifugation can be stopped.

Loading material into the centrifuge, material from the centrifuge unloading or anticipation of material delivery to the centrifuge or to processing the material in the centrifuge or for analysis. In the event that steps are taken to ensure that if the centrifugal rotor can be positioned and/or this position if it can be benefited. Rotor position, centrifuge a place (e.g., a container or a sign) with centrifugal rotor (212) such as holder or holding position a defined element that can rotate together), with this place is the angle of rotation relative to a non-rotating reference angle.

Positioning, recognizing the relevant position, positioning 'or position or fixation.

Centrifugal rotor control for positioning the rotor can be used. Preset a desired position on the remote in such a case, the control will enable the acquisition of the position and optionally kept at the specified accuracy angle. guarantees. In this way, for example, the individual processing stages Required cup holders or containers, possibly a straw unit, gripper or suitable as an analyzer position to interact with processing tools. can be brought.

Centrifugal drive 218 may be an Induction motor. This Typically, the induction motor is robust in terms of construction and cost-effectively, as well as trouble-free at high speeds It can provide the advantage of being suitable for work. However, a asynchronous motor compared to servomotor, required for positioning failure to have control data instantly available Based on the positioning difficulty due to is disadvantaged.

However, the asynchronous motor can be equipped with Vector control. Vector control, in the state of the art, for example A. Fitzgerald and et al., “Electric Machinery” (McGraw-Hill, 2003) explained in the post. Vector control, with Servomotor Provides comparable Asynchronous motor control. In this way, at high speeds and centrifugal rotor positioning it is possible to obtain an advantageous response.

Therefore, regardless of the arrangement described, a centrifuge for biological material, preferably a centrifuge Vector-controlled as drive element for the rotor Provision of a laboratory centrifuge with an asynchronous motor recommended. The asynchronous motor in question also has its rotor, to position the rotor at the angle of rotation around the axis of rotation. equipped with the relevant tools. Preferably, these vehicles includes a sensor for determining the rotation angle of the rotor. It also has a velocity feedback of positioning. It is possible to regulate it by means of an asynchronous motor.

A modified U/f characteristic controlled process is available. This In the process, the active current value is detected and a speed fed to the regulator. In this case positioning is active regulated using sinusoidal currents.

Mechanical Positioning of the Tip Centrifuge In addition, the positioning is non-rotating with the centrifugal rotor. and at least one rotatable with the centrifugal rotor suitable for interaction with the positioning counter element. can be realized using the positioning element.

The interference may be, for example, a mechanical engagement, a magnetic engagement, or sign can be read. First for interaction rough positioning, e.g. centrifugal rotor has already been carried out by command and so that the positioning counter element and the positioning It is beneficial that the element is in the range of interaction with each other. it provides. In addition, a large number of positioning counter-elements is also beneficial. For example, the centrifuge to be positioned arranged to correspond to all positions on the rotor It is beneficial to provide a positioning counter element.

A positioning element 450 and the corresponding an example of the positioning counter member (124) gripper explained in more detail in the description (see Figure 8); not attached to the gripper by persons skilled in the art. but, for example, the apparatus (1) in the centrifuge chamber (222) or other positioning attached to the work platform (2) against the elements and similarly described here The method of application of the elements is known.

Gripper and Pipette Unit Carrier Figure 6 shows the gripper unit (400) and pipetting unit (500) a carrier system (300) for moving shows the perspective view. Carrier system (300), a rigidly mounted X-rail (312) and a Y-rail (314). it does. Y-rail (314) X through a slide (318) it depends on the rail. Sliding element (318), X rail (312) in x direction It can slide along the Y rail (314) in the x direction. moves.

A drive to move the Y rail (314) in the x direction element 316 and drive element belt are provided. drive The apricot hangs from two reels. Drive element, from rollers can start someone's turn; turn, drive belt converted to progressive motion. For this purpose, rollers and drive Apricot is preferably in the form in which these pieces will interlock. i.e. the drive belt is a toothed belt. drive The belt is firmly attached to the X-ray with the attachment tools.

The coupling means are the X of the advance movement of the drive belt. It causes the Y-rail to move along the rail, that is, in the x-direction.

The carrier system 300 further includes a carrier member 320. (see Figure lc). Carrier member 320 Y rail in y direction is mobile throughout. For this purpose, the carrier element is the Y-rail (314). which can slide along the y-direction so that the carrier element movable to a sliding element. (328) (see Figure lc) it is attached. A drive element (326) to initiate this movement is provided. Slide (328) and drive (326), x similarly to the drive element (316) of the Y rail in the direction of is configured. The drive element (326) is attached to the Y-rail and it cannot move with the carrier member 320. drive element 316 and 326) are in the form of stepping motors so that the xy Correct positioning of the carrier element (320) in the direction of possible. Control of stepper motors, with reference to Figure l6 more accurately described. Flat ribbon cable (309), carrier connects the system 300 to a control unit; In Figure l6 connection between the respective interfaces (87la and 87lb) shown corresponds to the cable.

A suspension for the gripper 400 in the carrier element 320 tools (340) are available. Suspension tools, gripper (400) 2 allows it to move in the opposite direction. For this purpose, suspension tools Movement of the gripper (400) (340) in the z direction (see Figure 8) a gripper rail (345) to which it can be moved. Additionally for this movement, the drive belt of the Y rail (314) drive a lifting belt (342) similar to the drive member. An actuating member 348 is provided. Lifting belt (342), is guided on two rollers (346a, b) and the drive element can be moved through. Gripper (400), a attaches to lifting belt (342) with clamping hardware (443) and thus the lifting belt moves in the z direction as it advances.

In addition, the gripper carrier is attached to the pipetting unit (500) and related suspension means (350). Suspension tools (350), similarly to suspension means 340 for the gripper 400 allows movement of the pipetting unit in the z direction. In particular, the suspension means 350 include a lifting strap 352, a rail on which the pipetting unit can be moved in the z direction (355) (Fig. 7), rollers with which the lifting belt is guided (356a, b) and lifting in the z direction of the pipetting unit a lift drive unit for initiating its movement (358). These elements are attached to the gripper unit (400) similar to related elements.

Thanks to this structure, the gripper unit (400) and the pipetting unit (500) move together in the horizontal plane, that is, in the x and y directions can. In addition, the gripper unit (400) and pipetting unit (500) in the vertical direction, that is, in the x direction. can act independently.

Drive elements of Y-rail and carrier element (316, 326) drive of the gripper unit and the pipetting unit with elements (348, 358) are configured as step Hwtors.

Time the driven unit reaches the calibrated position. slot sensors (319) to detect for elements 348, 358; not shown) (see Figure lc), 329 (see Figure 6) is provided. Stepper motors and housing sensors similar to the description for the gripper (Fig. 8, 9) It is configured in the figure and depending on Figure 16.

Pipetting Unit Figure 7 is a perspective view of the pipetting unit 500. shows. Pipetting unit, attached to Fig. 6 that can run in 2 direction along rail (355) as shown. a pipetting member 501. Therefore, pipetting element 501 is a slider along rail 355 in 2 directions. pipetting with carrier, sliding or rotating element (545) suspension elements for the movement of the element (501) in the 2 direction (350) contain. it does. Suspension tools (350), a lift. apricot (352), rollers (356a, b) and lift belt (352) move an actuating member 358. lifting belt, It is attached to the pipetting element (501) by means of attachments (543).

The drive element 358 is similar to the drive element 316 of the Y rail is configured as follows. Drive element (358), rail (355) movement of the sliding member (545) along the can initiate movement of the pipetting body 501 in 2 directions.

Pipette tip for pipetting unit (500) pipette tips (730) holder 520. In Figure 7, a pipette tip (730), it is pushed into the pipette tip holder (520). pipette tips, it pushes detachably ie from the pipette tip holder (520) can be removed or placed here. pipette tip additional details on the construction of the holder EP-A-1862219 It is described in EP Patent Application no. Aforementioned The reference is a longitudinal pipette tip that extends in the axial direction. a device for holding with an axial fastener A plunger tube (518) on top of the pipette tip holder (520) is mounted. Pipette tip holder (520), inside of pipette tip Gas or pressure between the interior of the piston tube (730) and the piston tube (518). configured so that the change can be performed.

Piston tip (518) interior and pipette tip (730) sealing supplied attached to the outer part, so that the inner and outer Gas exchange between the sections only occurs at the pipette tip (730). pipette mouth 732. in the piston tube (518) The reduced pressure due to the outer part is transferred to the pipette tip and gas or liquid from the pipette nozzle (732) to the tip of the pipette. (730) causes it to be absorbed into the interior. In the piston tube (518) excessive pressure, similarly to the tip of the pipette tip of gas or liquid (730) from the inside out through the straw (732) causes strain.

Pipetting to create reduced or excessive pressure unit 500 includes a piston system 510. Piston system along a drive member (5l2) and piston rail (515b). A drive and piston carrier that can move in 2 directions (515a). The piston system is additionally (512) to the lifting element of the piston carrier (515a). a transmission system (514) for transmission.

The transmission system 514 is configured as an worm gear.

The piston system is additionally attached to the piston carrier with a fitting (517). 515a includes an attached piston 516.

Thus, the piston (516) can be operated without leaking the piston of the piston tube. It is placed inside the piston tube (518) to seal it.

The piston system is additionally composed of the piston carrier and thus a tool for detecting the calibrated position of the piston. the housing sensor (513). Slot sensor, description below similar to the slot sensor (428) of the gripper configured (see Figure 9). According to a preferred direction, The drive member 512 of the hoist is a stepping motor.

In this embodiment, the caliber of the piston is detecting the open state. each raised state of the piston, if possible caliber of stepping motor starting from open state can be determined by counting the steps. Shot with piston stroke or a fixed relationship between the volume of fluid removed available: Piston 516 and piston tube 518 provide a syringe. Above described in the EP Patent Application No. 06010976.6 pipette. tip holder directly onto the syringe, especially It is useful to attach it to the piston tube (518). This arrangement is allows a particularly compact design of the unit 500.

Immersion of the straw (732) in a liquid and the plunger (516) gas in the piston tube (518) increase in volume inside the plunger tube (518) and at the pipette tip. (730) creates reduced pressure. This decreasing pressure it passes through the mouth (732) and causes it to be taken to the pipette tip (730). The volume of liquid drawn is the gas contained in the piston tube (518). corresponds to an increase in volume.

The pipette tip 730 contains a liquid and the piston 516 If kept at a constant height, the liquid is at the pipette tip. The pipette tip 730 contains a liquid and the piston 516 gas contained in the piston tube (518) when lowered reduction in volume inside the plunger tube (518) and at the pipette tip. (730) creates excessive pressure. Excessive pressure, liquid from the mouth (732) allowing it to be removed from the pipette tip. The volume of ejected liquid is reduced by the volume of the piston tube (518). corresponds to the gas volume.

Pipetting element (501) for picking up pipette tip, pipette tip via a suitable pipette tip (730) in the station 720. can be moved with the pipette tip holder (520) (see Fig. 1 and Figure 12). Straw element 501, downward movement so that the pipette tip holder (520), approximately 10-50 N is strongly pressed down against the pipette tip. Later on eg pipette tip. outer circumference of pipette tip holder (520) here by squeezing the sealing element around the surface is kept. This type of sealing element, again reference described in EP Application No. EP-A-1862219 shown has been made.

To discard the pipette tip (730a) from the top in Figure lb. waste related disposal system as shown in the view (740) can be provided. The disposal system 740 is a large cross-sectional area. (744) and a mouth with a small cross-sectional area (746) (742). Wide cross-section of the area of interest (744), it is designed so that the pipette tip (730) can be pushed. Alanine The small cross-section (746) ensures that the straw tip (730) cannot be pushed. only so that the tip holder (520) can be pushed in. is designed.

Pipette unit (500) for disposal, containing pipette tip (730) large cross-sectional area (744) of pipette tip holder (520) is moved as it will be on it. Pipette tip (730) The tip holder (520) containing the pipette tip is lowered and the tip is it is pushed into the opening (742). Then the pipette tip (730) containing pipette tip holder (520), small cross-sectional area (746) is moved and the pipette tip holder (520) is (742) is removed. Pipette tip 730, eg an open field on the edge (744) is held by its upper edge and therefore remains below the opening (742). Pipette tip holder (520) When completely removed from the opening, the pipette tip (730) is detaches and the used straw is placed on the underside of the opening (742). It falls into the reservoir provided for the ends.

From this section the entire pipetting procedure is described. will be. For pipetting purposes, the pipette unit in question first selects a pipette tip as described above.

The selected pipette tip (730) is placed on the chamber holding the liquid to be drawn. It is moved by the straw body (501) and inserted into said chamber. right lowered.

During pipetting, knowing the fill level of the chamber or It is helpful to identify the pipette tip 730 before lowering it.

This is one of the fill level measurement methods described below. using one, for example, using the ultrasound unit (830) realizable. Then the pipette. tip 730, generally to the level of filling regardless of the arrangement described can be lowered into the rough based on This situation, for example greatly reduce the dependence on the formation of drops on the pipette tip. It provides the benefit of being selected to prevent it.

The desired amount of liquid is drawn into the pipette tip (730), the pipette tip (730) is moved over the target container and the desired amount the liquid is released from the pipette tip into the target container. Pipette tip, target If it is desired to prevent contamination with the liquid in the container, the pipette tip with the liquid in the target container or the target container wall or on the target container to prevent contact with the rim. height is beneficial.

Basically, to avoid contamination, the pipette tip is (730) is useful to change, otherwise the samples there is a risk of contamination or cross-contamination it could be. For this purpose, the pipette tip is used as described above. discarded to the waste disposal station 740 and again described above. A new pipette tip is selected as indicated.

Grip Unit Figure 8 shows a perspective view of the gripper 400. shows. Gripper, Z as shown in Figure 6 a gripper that can be moved along the rail (345) in the direction of element (4). For this purpose, the gripper element The carrier (445) for a rail (345) Suspension means (340), rollers (346a, b) with (342), and similar to the associated elements of the pipetting unit 500. includes drive element 348 (see description in Figure 7).

The gripper unit (400) is a device for gripping the container (160). the gripper (410). A detailed view of the gripper It is shown in Figure 9. Gripper, hard gripper slot contains. Two gripping arms (416a, 416b) in addition to the gripper contains. Each of the gripper arms is in the gripper slot (412) is suspended from a shaft (426a, 426b) and can be rotated around. Grab handles in closing direction when turned, the two arms (416a, 4l6b) the gripper moves together in the field and therefore the container (160) may grasp at the ring 168. Kabi from the ring (168) recesses (417a, b) to fit the ring (168) in the respective grip areas of the gripper arms (4l6a, b) is provided. A drive element for clutching the container 160a (420) is provided. The drive element is connected to the lift element (422). moves the course. Lifting element stroke, clutch presses the two upper limbs of its elements apart so that the lower limbs, i.e. the shafts (426a, b) from the upper limbs on your side. the found limbs are pressed together. lower limbs, includes a coarse grip area. Hence the description the movement is a movement in the closing direction and the lower arms Compressing it allows the cabinet 160 to be gripped.

To release the cabinet 160, the drive member (420) lift the lifting element (422) in the opposite direction, i.e. to the top in Figure 9 It should move right. In this way, your two upper arms limb (416a, 416b) closer to lifting member (422) is suppressed. As a result, the spring or elastic element 424 can press the upper limb further together and push the two lower limbs further can separate. Thus, the gripper moves in the opening direction and the cap is released.

In the corresponding figure, the elastic element 424, the gripping arms 416a, 416b) is shown as a compression spring in the lower limb area.

However, in the upper limb area of the spring, for example, the elastic tensile element Alternative arrangements that it takes the form of are also possible. Elastic of the tensile member to the lifting member (422) unintentionally. Be careful not to hit it. This is, for example, strain elastic covering the two upper limbs of the member. in the form of a ring possible by creating

Grabber (410) to detect the opening state of the gripper also whether the gripper in question is open or closed. that outputs a sensor signal containing information about the absence of the housing sensor (428). For this purpose, the slot sensor (428) e.g. the optical path of the gripper based on the gripper state may contain a beam of light that may or may not be cut by the limb.

So that the slot sensor changes its state, so for example state interrupted by the transition state of the optical path of the light beam detecting a caliber open condition that varies between can be done. Caliber on state in a possible arrangement For example, it is clear that the gripper opens to the maximum length. According to a preferred direction, the actuation of the hoist element 420 is a stepping motor. In this arrangement, the gripper if the caliber open condition can be detected, every open. status, starting from calibrated on state can be determined by counting the steps of the stepper motor. Shape Other slot sensors, for example, as shown in Fig. function is similar.

The gripper 410 further includes a cover cage 414.

Lid holder (414) firmly in gripper housing (412). it connects. Lid holder (414) is a latch for container lid (166). provides a stop. Cover (166), cover said cover (166) connects this component to the container 160 and therefore a fixed a link that positions or holds against The element (164) is elastic. stop with force. against the piece is suppressed. Clutch levers (416a) additionally have a recess and the connector (164) is formed from this recess. may be prolonged. Via this recess and cover holder (414) it is possible to guide the cabinet 160 connected to the gripper 410.

However, the gripper is also a container without a cover 166. (160) can comprehend. In such a case, routing is not provided.

Referring again to Figure 8 , the gripper 410 is a 'shaft 436a' It is mounted in rotation around its periphery. Gripper (410) rotates the device 430 can be rotated about the shaft 436a. rotary device (430), a rotary drive member (431), a drive gear (434) includes an additional gear (436) and a seat sensor (438). it does. Rotary drive (431), drive gear (434) arranged to move. Drive gear (434), it passes to the other gear (436). Gripper (410), other gear (436) is rigidly connected and with this gear the rotation shaft (326a) can spin around together.

Rotary drive 431 preferably as a stepping motor configured so that the gripper 410 the angle can be adjusted around the rotation axis 436a. nest sensor (438), whether the gripper (410) is original or calibrated. provides information on whether it has returned to its desired position, and so there is a tool for controlling the stepping motor (431). creates a calibration point. of the calibration point function of the clutch levers (416a, b) described above. similar to the function of the calibrated on state. gripper Rotating the gripper 410 preferably at an angle of at least 120°. It is formed so that it can be rotated with the device 430.

The gripper 400 is additionally attached to the cup holder of the centrifuge 200. 120 for a positioning device 450.

The positioning device 450 includes a positioning tip 452. it does. The locating tip is attached to the corresponding cup holder (120). It is formed in such a way that it can pass into the positioning hole (124).

More generally, the positioning element 452 is the container. holder (120) to the positioning counter member (124) with this component, which may pass through or, for example, by electromagnetic force. are created in such a way that they can interact. Positioning of this type with a positioning opening for the The container holder is shown, for example, in Figure 10. This pass, of the gripping element (401) attached to the container holder (120) ensures that it is in one position.

The positioning device is additionally connected to the positioning tip (452). a positioning member 451. Positioning element 451, independent of gripper element 401 in the Z direction It can move along the positioning rail 456 as This positioning element (451) for purpose positioning in the Z direction to a positioning carrier that can slide along the rail (456). (457) is connected. In addition, the positioning element is related with a positioning spring using rig (458a, b) 458 is coupled to gripper member 401; positioning spring (458), positioning member (451), gripping member (401) It provides a resetting force for its connected motion.

Positioning spring, positioning during car engagement positioning counter member (124) by the tip (452) sufficient force to be applied so that the two It ensures that the engagement between the elements is guaranteed.

Preferably said force is between 1 and 10 N, preferably between 1 and It is between N.

Regardless of the edit shown, unpublishing spacing (ie, the gripper element in the 2 direction (401), position element (452) of the positioning spring (458) into the cup holder that he presses against the positioning counter member (124) (120) connected height distance), one cabinet (160) one container from the holder (120) or into the container holder. in such a way as to meet the requirements at the time of installation. For example, if the lifting range is a cabinet (160) be removed from or placed in this position. from the cup holder, containing the not completely removed or partially inserted into the holder. positioning with positioning element (452) along the entire phase that a crossover may occur between the opposing element (124) It is beneficial to be selected.

The gripper (410) is for example the following on biological fluids. suitable for gripping containers: rotary tube (160), Eppendorf tube (170) or similar without cap vessels: said vessels with grip levers (416a, 416b) understood from the outside. Optionally one or more a number of recesses (417a, 417b) engage the ring of the containers. similar to lidless containers with additional optional lid holder (414) covers (166, 176) as described above preserves.

A container for biological liquids, where the liquid in question as a component intended to receive and contact this liquid is defined.

In addition, the gripper 410 and the cup holder 120 It can be configured so that it can grip the cup holder.

For this purpose, part of the container holder is pushed through the gripper. It is designed to grasp. For example, shown in Figure 10 unlike the cup holder, a holding position (150) is provided with a ring. can be equipped and the gripper can grip the ring like a container.

Meanwhile, the cup holder is preferably the optional lid holder. (414) so as not to interrupt gripper operation. is designed. Lid with suitable configuration of cup holder The holder is also a position defined, for example, by the holding position (150). of the cover holder, such as the angle of rotation around the axis. can be used to define routing. gripper, one container (160) is suitable for placing in another container (170). This insertion, cabinet 160 into a holding position (130, 150) carried out in a similar way to placement. Preferably, the other cup 170 is in the holding position of the cup holder 120 (140). Preferably the cup holder 120 is in a centrifuge 200 is positioned. Gripper, one vessel (160) from another vessel it is suitable for removal (170). This removal process requires cabinet 160 similar to removing it from the holding position (130, 150). is performed. Preferably, an additional container (170), container holder (120) is in the holding position (140). Preferably the cap holder 120 is positioned in a centrifuge 200. Moreover The additional container (170) is in the holding position during the removal process. It is useful to provide an apparatus for fixing (140).

The apparatus in question is, for example, on the upper part of the cabinet or on the side. contacts an additional container (170) and presses the container downwards. can take the form of a compression element (not shown). Promise the clamping element in question, for example the positioning element 452) can be pre-tensioned by a similar spring or can be integrated with the positioning element 452.

The drive elements of the gripper as described above (420, 431, 441) are defined as stepping motors. nest sensors (428, 438, and 448), the caliber of the driven unit available to detect when it has reached a location. is done. Driven by counting the steps of stepper motors detecting each position, starting from the calibrated position of the unit. possible to do. Slot sensors (428, 438, 448) light designed as beams. to calibrated position when it is reached, the optical path of the light beam is refracted. nest sensors alternatively when the calibrated position is reached as light beams in which the optical path of the light beam opens can be designed or contact sensors or other sensors can be created as

Figures 13 and 14 show how to heat samples or liquids and shows representations of a unit 600 for agitation.

The unit in question is, for example, the lysis phase of a procedure. can be used for implementation. In addition, the sample other procedure that needs to be heated and/or shaken can be used for stages. At the same time, the unit Provides defined holding positions for biological material.

Therefore, the unit for heating and shaking (600), It can also act as a sample holder in the apparatus.

Heating and shaking unit as shown in Figure 13 (600) work platform for work platform It is equipped with an additional piece (602) according to its height. Unit (600) a container with an additional 12 container holding positions (612) carrier (610). Container holder, container to hold is adapted and can be manually inserted into the unit 600. Various different types of containers if other container types are used Carriers can also be provided. This particular procedure a suitable special container holder can be inserted into the unit (600). This container for easier classification in case of has numbers on its carrier (as “2” on the container carrier. (see Figure 14b, numbered).

The pot holder (610) shown is two rows of bottom pots. for 12 reaction cabinets with optional lids arranged in designed (eg Eppendorf cubes or other containers).

A row, as shown in cross-section in Figure 14a, ie the bottom holding position is for the 1.5 ml format while the other row is designed according to the 2 ml format.

On the vessel carrier 610, remove the lids of the reaction vessels. There are cover holding positions (616) for picking up. Lid holding positions (616) additionally e.g. slot, recess or pocket can take the form. The container carrier 610 is designed to be used with holding containers or placed together with the contained samples, removable. As a result, the container carrier (610) can be loaded in. Container carrier, holding containers with bare hands It can be created in such a way that it can be attached and removed. Alternative As two labware, the containers are stuck on top of each other. so that it can be inserted and removed using gloves configurable. Also, do not machine the containers. so that it can be inserted or removed is created. In this case, the lid holder, if provided positions (616), any cover provided in the gripper (410). holder (414) (see Figure 9) so that the covers can be accessed. It is useful to be designed in such a way. Container with 12 holding positions The carrier (610) can parallel processing up to 12 different samples. allows. Parallel processing of different samples container to eliminate allocation errors in case of A clear positioning of the carrier is helpful. Net an apparatus for positioning, for example, in Figure 13 is shown. In said apparatus, the container holder (610) a guide element (618), such as a rail, recess, or slot provided and oscillating frame (634), a counter element (619) that the cup holder (610) is attached to the oscillating frame (634) only It is mounted in such a way that it can be placed in an orientation.

Also in Figure 14b to eliminate allocation errors. numbering of the holding positions (612), as shown It is advantageous to be labeled via (613).

Figure 13 also includes a heating element (622) and heat transfer. illustrates a heating system comprising element 626. Heat of the transfer element 626 and the cup carrier 610 in Figure 13. heat transfer as shown in the specified arrangement element (626) into a container placed on the container carrier (610). direct contact, heating increases the efficiency of the system. Preferably heat transfer element (626) A>3O W/(m K K), most preferably Ä>100 W/(m x K) thermal conductivity is available. Heat transfer element (626) eg It is connected to the heater (600) by screw connection. container formatine element (626) can be replaced in case of change- Heat transfer alternatively, the element 626 is a part of the container carrier 610. can be created as part of In this case, the container carrier This part is thermally conductive, such as a thermally conductive metal. May contain conductive material. In this case the container format when replaced, it includes only the cup holder (610) -70a-.

In the direction shown, samples can be heated up to 70°C.

Heating time for the temperature range from room temperature to 55°C about five minutes. placed on the container carrier (610). temperature accuracy required for liquid in a container is +/- 3 K (at 55°C). Temperature accuracy, for example at target temperature a result of deviations or temperature difference inside the heater with different deviations in the target temperature of the liquid electrical heating element. Two separate heating in this element circuit is provided. An advantageous combination of the heating circuit force 70 W for the first heating circuit, the second 30 W for the heating circuit.

A temperature sensor such as type PT100O with temperature regulation value can be read. Heating depends on heating time and temperature of the above-mentioned specifications regarding the accuracy sizes available. connected in series in the power circuit A temperature safety device is available. This device itself won't reboot. Any active in the arrangement shown there is no cooling.

The pot holder (610) also has a hand guard and heating system. 620 can be designed as a connected thermal insulation. This In this way, the risk of burns to the user can be eliminated or can also be reduced. For example, in the container carrier A<5 W/(m X K), more preferably a handle with a thermal conductivity of A>2 W/(m x K) part may be available. In addition, the container holder (610) e.g. of a thermally insulated material such as ceramic or plastic may be the product. This is the isolation of properties. heat loss It provides an additional advantage in the form of reduction. Used plastic eg at 121 °C for 20 minutes an autoclavable plastic that tolerates autoclaving it could be.

The lysis step of some procedures is the mechanical separation of the samples. requires shaking. The arrangement in Figure 13 is for this purpose. an agitation system integrated into the shaker/heater (600) (630). Said agitation system (630) is attached to the apparatus. (1) an attached drive block (636), with said drive block (636) attached to work platform (2) about to swing an adjustable movable oscillating platform (632) contains. The vessel with the heating system (620) described above the carrier (610) connects said elements with an oscillating platform. It is mounted on the oscillating platform so that it oscillates together. is done. The block in question is in the form of a cross of four separate windings. to create a churning movement that involves the regulation of includes an electric drive element for These are a It works like a stepping motor. However, the drive element is desirable It can be any other electrical actuation element that is used. Agitation, movement, weight. around the center an oscillating motion, a rotational motion, or their or combination of similar movements is meant.

In the present aspect of the invention, the agitation system 630 is a rotary formed as a shaker. This formation is approximately 2 mm (top to top) agitation amplitude and 2000 Maximum agitation time above RPM or allows speed. The shaking motion of the samples splatter and, accordingly, cross between samples in order to remove contamination as much as possible. is designed. Therefore, the shaking motion is in one plane. occurs, partially vertical component, includes 'or no vertical component' knowledge does not contain. In the agitation system, to the work platform drive block (636) and attachment (602) for working fixed, attached to its platform; shown in Figure 13 other elements are shaken. of splashes that may occur during shaking movements. sample to be caught and not cross-contaminated. Providing a separator or shield for containers it is useful. According to the appropriate design of the container carrier 610 a shield with separating elements, for example separating walls obtainable. Separator elements of this type are shown in Figures 13 and 14. is not shown. These are in the cup holder. (610) integrated separator walls between adjacent cup holding positions (612) can be manufactured. The height of the separating walls, the container, Cup holder when placed in one of the cup holder positions at least the upper edge of the cabinet provided for corresponding to its height. may come. Preferably separator wall height is 3 to 7 mm higher than the height of the cabinet top edge. is too much. Alternatively, the container holder (610) can be placed. other. an additional piece. by. a separator can be created and this separator provides separator elements.

Preferably, the separating elements consist of the pipette unit 500 and/or will enable the gripper 400 to gain access to the containers. is created in this way. In addition, the defined oscillating platform It is useful to be able to stop at a standstill. For example, oscillating platform in the shaker up to a stop able to bring and defined against said stop an electromagnet that can be positioned at rest editable. Alternatively, for example the drive of the gripper stepper motors with slot sensors similar to said drive block containing can be achieved by means of positioning. arrangements are also possible. For example, in any case other system Shaker system only if use is not required without heating element or heating element only A free rinse system may be used. Other functions can also be added. For example, cool, juicy, A cooler such as a thermoelectric cooler can also be used.

The said refrigerant is also combined with the centrifugal chiller. (230, 250) can be combined (see Figure 3).

In addition, the arrangement of the holding positions 612 has been changed. can show. Figures 13 and 14, 6 ;ç 2 matrix of holding positions shows another one with n x m holding positions. arrangements are also possible. Other possible alternative arrangements, for example a circular or hexagonal arrangement The holding positions (612) of the container carrier (610) holding positions in the centrifuge (200). to layout of locations It is particularly advantageous if it is arranged in a corresponding manner.

The advantage of this situation is that the holding positions of the cup carrier 610 (612), the coordination of the holding positions in the centrifuge especially easy. In particular, the container carrier (610), as a sample holder for samples to be processed used and the centrifuge provides the samples to be processed The risk of assignment errors is reduced.

Arrangement of handle positions (612), Figures 13 and 14 with the heat transfer element 626 in the embodiment shown. determines. For example, alternative heating using a liquid bath system or a heat transfer element integrated into the container carrier larger relative to the arrangement of the holding positions 612 allows flexibility.

Consumables Station Figure 12, consumable for consumables (700) shows a perspective view of the station; Consumption materials station, a hopper for process fluids station 710, a pipette tip for pipette tips 730 station (720) and vessels containing process fluid (714, 716). Contains other holders for

Hopper station 710 for process liquid, process liquid It includes six holding positions for chambers 712 containing it.

Different process fluids can be used based on the procedure.

Examples of process fluids used are “bind-wash-separate” are buffer fluids for the realization of the stages.

Hopper station 710 plus consumables 700 from the consumables station for the corresponding station (710) a handle 718 for its removal. Aforementioned handle (718) additionally to the hopper station (710) to the corresponding station. (700) stipulates a clear routing in which it can be placed.

Consumables Station (700) plus process fluid holding positions for chambers 716, 714 containing contains. Said chambers are connected to the respective chambers (712). process is similar but only sparingly used aimed at civilians. Stations (714 and 716), related The station 714 contains lid holders for the container lid. differs in that. So for example Eppendorf tubes can also be used here as containers.

Conversely, any cap holder at the respective station 716 is not available, so the station in question is only for containers with or without a screw cap used. Additionally, Figure 12 shows the tip station 720.

From the consumables station for consumables removable. Pipette tip station (720) to pipette tips (730) It includes several holding positions for Related figure, for example two, which differ in the quality of the pipette tips they hold. shows the pipette tip station 720. For example, two straws The pipetting volume of the pipette tips in the tip station is different. it could be. Pipette tip station 720 It contains signs (721, 722) that allow it to be recognized. This marks are detected, for example, using an optical sensor. are created as protruding elements. every sign, whether the protruding element is present (1) or not It can carry one bit of information based on (O). Therefore said elements (721, 722) are two bits of information, ie 1 to 4 carries information that corresponds to a number between them. Therefore distinguish four different states that can be read in the arrangement shown Three of these situations can be used with different types of pipette tips. is relevant; the fourth case is “no pipette tip available” indicates the status. The alternative sign (721, 722) form is also possible. For example, different light with a surface that absorbs or reflects Marking can be done by coating. The nature of the sign also e.g. color coded or magnetically readable can be changed by coding. The coded bit shown in Figure 12 the number is also variable. For example, one, three, four or or a number of other signs may alternatively be provided.

Said elements (721, 722) are located in two of the pipette tip stations. such that the point along its short side is symmetrical is designed. As a result, the signs are anti-rotation mounted so that the consumables At station 700, there are two possible ways of tipping station 720. regardless of which redirect you use readable. Figure 12 corresponds to the bit order of 1-1, that is Indicates the type of beacon for which both marks are present.

The pipette tip station 720 also includes recesses 724 and strips 725. Stripes (725), indentations (724) can be placed and two as shown in Figure 12. be able to interconnect the neighboring pipette tip station 720 is designed in this way.

Pipette tip station (720) plus tip consumables manual insertion into the station (700) or two opposing handles that can be grasped for manual removal element 726. During the insertion process, the handle The hook portions arranged under the elements 726 are The materials are attached to the Station (700) with a latch. Extraction In the process, the handle elements (726) are pressed together and the hook elements can be released.

In addition, the consumables station 700 to remove the station from or to the work platform. also include holders 708 to facilitate insertion.

Waste Station Below the waste disposal system 740 shown in Figure 1 for example, used pipette tips, columns or other containers There is a hopper for receiving such wastes. Also waste on the working platform for taking the liquids (not shown) a receptacle accessible through the opening has been provided.

Disposal of pipette tips is described with reference to Figure 7. is being done. All kinds of other waste, to be destroyed it can be thrown into the receiving hopper through the opening (748). For example, to the process the liquid used for the purpose and no longer needed (748) may be released, for example, may be pipetted out. Moreover, Containers that are no longer needed, such as filter bowls, can also escape from the opening. (748) can be destroyed. The various receiving chambers are preferably separate chambers and a common It is arranged together in the waste receiving apparatus in the form of a hopper. Taking apparatus so that it can be removed in one piece. can be created. For example, the apparatus in question is shown in Figure 2a. of the screen as shown for the station 750. 910 may be in the form of a drawer that can be removed from the bottom.

Controls and Sensors Figure 15 is an extremely simplified schematic drawing. operating the biological material processing apparatus in case of shows. The controller consists of a computer (870a'), an input and output unit (870b'), intended for communication with external equipment interfaces (870c') and motors, stepping motors, light beams interfaces (862') to the control parts of the apparatus (l) such as contains all of the said components on one card. (870') or spread over several cards.

The computer (870a') is a processor, working memory, operating system, preferably with an embedded IS and various control It contains programs to run its functions. One or more protocols and words in working memory parameters to run the protocols in question. additionally recorded.

The input and output unit or user interface (870b') is a graphic controller for controlling the display 910 and graphic display, for example keys and/or touchscreen It contains an input interface for receiving inputs of the form it does. Said interface (8700') is for example an Ethernet interface, a USB interface, an R8232 interface or some other classic can be interfaces. Interface (870c') plus a Web server may contain.

Interfaces (862') to be controlled like stepping motors convenient control interfaces for various pieces of equipment contains. Various controllable items shown in Figure 15 the unit is listed in the description in Figure 16.

Figure 16, Biological material processing by sampling method a more detailed view of the apparatus controller shows. For example, the control unit shown in Figure l6 It can be used in the apparatus shown in figure lc. In this case The motherboard (870) is located in the main body of the apparatus, that is, the working It is located under the platform (2). Y board (874) Y rail (314) arranged below. Transport card (872) of carrier (320) arranged on it. Gripper board (876), gripper The pipetting card (877) is arranged on top of the unit (400). It is arranged on the pipette unit (500). Centrifugal card (878) on top of centrifuge unit (200). has been arranged. The cards mentioned above are in Figure 15. corresponds to the specified computer board (870'). main board (870) a mainframe (870a), a graphics controller interfaces to the various apparatus units to be controlled (862, 864). Additional carrier board mounted via a transport card via a counter interface (871b) 872 includes an interface 87la for communicating.

Host (870a), graphical interface (870b), and interfaces (870c) corresponds to. The following functions in interfaces (870c-e) available: interface (870c) that controls a USB host protocol USB is the host interface. Therefore, that interface (870c), host (870a) memory eg USB sticks Allows the installation of new protocols via For example The (870d) component is a series that can be used for maintenance purposes. interface (R8232) icons. Alternatively, the interfaces (870c and d) be combined and desired, such as Ethernet or TCP/IP. Any protocol can be used. Interface (870e) (R8232) communicates with driver board (878) of the centrifuge.

Host (870a), directly or interfaces (871a and 871b) pertaining to stepper motors and light beams through controls various interfaces 862. Light. general beams such as slot sensors for stepper motors i.e. the stepper motor driven part is a defined in the calibrated position, for example, motion the blocking part connected to the part to be moved, the light beam a signal or signal change in the sensor when it breaks it occurs. Alternatively, the said light beam it may also be attached to the part to be moved.

In this way, the mainframe 870a, in particular the X of the Y rail movement on the track (316, 319); on the carrier's Y-rail (326, 329) to his movement; along the Z-axis (441, 448) of the gripper to your movement; rotary movement of the gripper 420, 428; gripping action of gripper arms (431, 438); pipetting Z movement of element (541, 548) and pipette piston drive respective stepper motors for the movement of element 512 and controls the slot sensors. The host (870a) also movement of the motor towards the heater/shaker (636). checks. Slot sensor required for this motion is not; which can only be controlled by an interface an optional light beam (850) for other purposes can be used. Other light beams (850, 852) eg for consistency checks and other troubleshooting. can be operated by interfaces.

In addition, the main computer is designed to ventilate the centrifuge. heaters with motors (234, 251) and another interface (864). temperature element (622) and heater/shaker (600). It controls the sensor (624). For different stepping motors various interfaces via different boards (870, 872, 874) is distributed.

In addition, host (870a), corresponding interface (870e) The driving element of the centrifuge (200) via electronic checks its components (878). Driver electronics (878) to control centrifugal motor (218), centrifuge cover (240) and, for example, cover lock (269) with a suitable for opening with opening mechanism 260 (not shown) Contains basic control tools.

Also on the gripper board (872) with the host (870a) A separate computer (872a) communicating is provided. Promise subject computer (872a), associated interfaces (866, 867) via an optical sensor (810a) and a light source a light sensor (810) (see also Fig. 7), including (8lOb), an ultrasound unit comprising an ultrasound sensor (830a) (830) and to control an ultrasound source (830b) used. Computer (872a) plus other stepper motors Contains drivers for

The apparatus in question, the apparatus and the apparatus load various Equipped with sensors to detect the status of it is advantageous. For example, the sensors are one of the following or suitable for performing multiple functions: pipette tips 730, for example a pipette tip in the provided holding positions for the ends, such as the hopper 720 detecting; container holders (120) for holders detection at locations located in the centrifuge, i.e. the container in the centrifuge detecting the positions occupied by the holder; 716), for example in the centrifuge (200), a in the heater/shaker (600) or in a hopper for this purpose, in a container holder (120) at the station 710. to be made; to compensate for any mechanical tolerance e.g. carrier (300), gripper (400) or pipette unit positioning moving parts such as (500); detecting the zero or slot position of the stepping motor; measuring the fill level of the liquid or other Ingredient; a cabinet, for example a column 160 or a pipette tip. (730) determination of qualification and/or capacity. Cover such as, for example, the centrifuge cover (240) and the apparatus cover equipment condition, such as opening, or filling of the waste bin. checking level. In particular, many of the above-mentioned functions and other Providing sensors suitable for performing functions it is advantageous.

In a preferred embodiment, one or more of the aforementioned more functions, carrier (300), gripper A light that can be attached to the (400) or pipette unit (500) by the sensor (810). For example in Figure 6 In the embodiment, the light sensor 810 is attached to the pipette unit. Conclusion as a light sensor, part of the work surface (2) It is movable on the sensor and the height of the sensor can be adjusted.

Preferably the light sensor is in the red or infrared range. as a radiation method with frequency. a laser or light cone and/or external light to signal modulation to suppress the input and darken the background with triangulation. equipped with tools. Therefore, the light sensor the presence of material with specific reflective properties, especially in the detection range of the light sensor. to disperse the reflection in the range of light frequency can be detected accordingly. Pipette tips, columns, container holders or other consumables. to the objects or types of objects in question the presence or absence of objects in the position of apparatus for the diagnosis of its absence is meant. can be performed for diagnosing a large number of objects. This In this case the sensor point is line by line in various positions, for example is advanced. Measurements at suitable positions during movement makes. between positions for objects and the sensor. sensor position if mechanical tolerances are too large first after this chapter. can be adjusted as described.

If the measurement is taken during movement, the measurement time is short. occur, preferably within the range of a few milliseconds, possibly 2 It should be between 100 and 100 ms. Alternatively, the sensor motion can be stopped for the detection process.

Sensor* motion indicates that the sensor recognition range is to a particular object, for example a pipette tip or part of a pipette tip. It is selected to cover the points or locations provided.

If possible, make sure that the sensor point is possible during the measurement. It should be guaranteed to affect the feature to be recognized as accurately as possible. For example, if pipette tips are present point should not touch the edge of the pipette tip, but should It should descend as steeply as possible.

Recognition range preferably to the part of the object that increases the recognition is directed. Increased recognition, “object exists” and “object does not exist” is defined as the increasing signal difference between

Therefore, the item with increased recognizability, any object signal detected at the relevant location when not available reflection characteristics that differ from the or several other signal behaviors. of the light sensor or preferably the light beam of some other radiation source part with increased recognizability visible in the direction of the sensor point not exceeding 1.5 times, l times or 0.7 times its area has size. In addition, the track with increased recognition It is preferable to be positioned at a defined height depending on the sensor. Increased recognition if pipette types are available the part may be a fleece that is mounted on the pipette tip. Promise The fleece in question is usually white but can be of any other colour.

Increased recognizability if cup holder is present part, for example the container provided with a particularly reflective material It could be part of the holder. Columns (rotating tubes) available a piece of membrane with increased recognizability in the event of a The filter can be membrane or Fliess. For example Eppendorf increased recognition if containers such as tubes part with a flip cover, a hinge for the cover element or stabilizer element.

If containers or columns are present, of the components at the desired liquid level, in the desired color. It should be taken into account that it can be filled with liquid.

A felt in the columns can be in any desired color.

Therefore, the background for the detection process is darkened. It is preferable to use a light sensor. This is the first distance and then column or first distance is moved over the container at the second shorter distance.

The methods and other methods described above are shown in Figure lc. checking the loading of an apparatus as shown is suitable for For example, the completeness of the apparatus in question or consistency can be checked. For example, centrifuge (200) or the sample holder into the heater/shaker (600) Different container holding to control the loading of the apparatus Check if a cabin is available for their location. saving is advantageous. Therefore, in the container holding positions the total number of currently registered containers can be determined and can be saved. For example, the container in the centrifuge rotor (212) the total number of containers in the respective holding positions of the holder (120) number can be detected. From the total number of containers and their from their positions, for example the cup holders in the centrifuge (200). The number (120) can be deduced or separately can be saved. Available in cup grip positions containers based on the total number of containers recorded its distribution in the holding positions is also detected. For example, centrifuge (200), as described in the previous sections. to minimize any imbalance in the loading a condition may be made. Available in cup grip positions the distribution of containers recorded as A check for compliance can be carried out.

As explained above, a cabinet in the pot-handle positions an optical device for recording the presence or absence of sensor can also be used. Therefore, in addition optical sensor, sample cup positions can save the number. Sample container location may be present in the shaker/heater 600. Therefore, of the number of sample containers currently recorded. It can be checked whether it coincides with the total number of Each sample, such as a container holding position in the centrifuge 200 an allocation that assigns a container holding position to the cabinet realizable.

Allocation, eg a sample booth, with booth available you can assign a container hold position where it is saved, or the cabinet, present in at least one container holding position of the group a group container holding position in a sample cabinet. can assign. Allocation, sample cabinet assigned to container holding position instructions for positioning a sample cup can also be edited. For example, the sample container location, may be allocated to a container holding location by a common name or of the sample cup positions at the sample cup station. of the container holding positions in the centrifuge. allocation on the basis of the corresponding regulation can be done. The allocation also includes the respective container holding position, sample a table that allocates the cabinet to the sample cup position can also be created.

Optical sensors are also included with the consumables of the apparatus. makes it possible to control its loading. The said consumable materials can be containers or biological materials. Especially these are pipette tips, rotary tubes, Eppendorf tubes and biological fluids, enzymes and/or buffer fluids it could be. For this purpose, ready-made samples containing samples for processing the number of sample containers is recorded; relevant in certain situations depending on the protocol, the number of sample cups prepared required number of samples to process samples based on and/or the amount of consumables is determined; available in the apparatus the number and/or quantity of consumables are determined; the number and/or amount of consumables available in the apparatus, with the number and/or quantity of consumables required are compared. Actual condition of consumables user-directed references, desired created to generate the state~ and on the monitor (910) is displayed.

To adjust the position of the moving parts, one or more Multiple light sensors can also be used. preferably the word subject light sensor, positioning objects used for detection. In one possible embodiment, one or more multiple light sensors eg carrier (300), gripper (400) or a movable unit such as the pipette unit (500). attached so that it moves with the unit. of the unit the sensor is moving over a beacon and the location of the said sign is defined and known. This In this way, the sensor and thus the position of the moving unit can be detected. The mark is light at a defined position of the light sensor. triggering the signal or signal difference in the sensor element. For example, the mark may appear on the work surface (2). can be created with an edge, so work on one side of the edge surface (2) is within the detection range of the light sensor. is not in this range. Alternatively, the sign two surfaces with different diffuse reflection characteristics can be formed by a boundary between in various directions in a number of directions to allow positioning extending edge or nerve (for example, two directions perpendicular to each other) can be used. However, due to its structure like a light sensor the presence of a sensor of a rotationally symmetrical nature positioning in different directions is typically different. has degrees of accuracy. In this case, in different directions multiple directed light sensors or rotary sensors can be used. movable depending on the relevant position mark the position of the part, by counting the number of steps of a stepper motor can be determined. For example, the corresponding positioning will be backup can be designed in . Positioning, mechanical tolerances can balance.

Other sensors and markings are also for positioning. can be used. For example, the sensor is a magnetic coil, camera or It could also be a light beam. In this case, the signal is for example inductive. conductive element intended for a colored mark or light beam It could be a blocking element. For example, by stepping motor moved, at the defined caliber position of a part light beam to detect if (slot location) can be used. This type of sensor (438) is shown in Figures 8 and 16. described with reference. Alternatively, it the sensor can be fixedly mounted and in the movable part location, mark can be provided. For example, with reference to Figure lc, centrifuge rotor 212 or cup holder 120 positioning mark can be provided. In this case the centrifuge rotation angle of rotor 212, using optical sensor can be positioned.

Centrifuge rotor (212) or another with positioning marking. a movable device to position some moving parts. sensor can also be used. For example pipette unit (500) to a sensor unit that can be moved by a stepping motor A mounted sensor can also be used. In this case positioning of the moving part in the following steps is performed: Position of sensor unit moved by stepping motor calibrating, i.e. by means of an optical sensor the socket of the sensor unit by reading a fixed mark. creation of position. Sign, e.g. study can be provided on the platform (2). The sensor unit is movable move to a defined position in the part's motion range (eg in the case of centrifuge 200); defined location, number of steps of the stepper motor of the sensor unit. obtained by counting.

Moving the moving part, eg optics the sensor is a mark on the moving part, i.e. possibly rotating with a centrifugal rotor. that the mark is in a defined relationship with the optical sensor. rotating the centrifuge rotor (212) until it detects it.

Calculation of the moving part from the defined relationship.

Alternatively, the position of the moving part can also be movement of the unit over the range of motion of the moving part. can also be detected by Meanwhile the sensor The position of the unit is counted by the number of steps of the stepper motor. is updated. The optical sensor is a piece on the moving part. that the sign is in a defined relationship with the optical sensor. the position of the moving part when detected can be calculated from the relation.

Containers for biological materials, especially liquids a fill level monitor to check the fill level device is advantageous because this type of fill level monitoring supplying the apparatus with a sufficient amount of buffer, without is left to the user's responsibility. Error risk and user The control heat can be reduced by

Also, filling other liquids or solids such as powders levels can also be checked. In particular, relevant Guaranteed fill levels to be within a certain range. can be done. The existence of the said substances is also described below. can be checked using the methods described. For example, filter in the centrifuge using a fill level monitoring device The filling level of the containers (rotating tubes) is too high. possible to be guaranteed. As a result, the filter A warning may be given if the containers are overfilled. This ensure that the filter pans are not blocked during centrifugation. prevention is possible.

Various methods for measuring the fill level of a liquid can be used. To prevent cross contamination The contactless method is particularly advantageous. Fill levels some non-contact measurement methods via the following example explains: In the optical measurement of the transit time, light excitations are used in an optical is fed into the hopper via the fiber. reflected from the liquid surface with a light photodiode. detected. between stimulus and echo delay is a measurement of fill level depth. ultrasound measurement during ultrasound excitation, for example a piezo-ceramic element It is propagated by a piezo element such as reflected from the liquid surface excitation by the same piezo element after the delay is taken. The measured transit time is the distance between the sensor and the liquid surface. is the measure of distance. Preferably about 250 kHz transmission using a sensor with a resolution of approximately +/-l mm at the frequency preferable. Also using a reference surface It is preferable to calibrate the system.

During capacitive measurement, the sensor's switching point capacitive sensor head against the liquid surface until it is reached is moved. Capacitor electrodes of the sensor head, close to the surface, the switching point is reached.

During the optical measurement of the transmission, the laser wavelength, filling that the laser light will be absorbed based on the level of is selected in the sequence. For example, wave in the region of about 1300 nm size is advantageous. Accordingly, absorption filling measure the level.

In the optical measurement of angles, the laser beam is applied to the liquid surface. is reflected. The angle of reflection and therefore the liquid surface, optical determined using the line sensor. In this process, the material surface is illuminated by laser or some other radiation source; liquid or liquid (or solid) surface projected onto its surface radiation diffusely back-radiated by a measured by a radiometer; measured by radiometer radiation source, radiometer and vessel are spatially related to each other (e.g. radiation source by bending), so that radiation with a vertical beam to the surface of matter between the first beam passing from the source to the material surface. the first angle, with the beam perpendicular to the substance surface, from the substance surface the beam reflected by the first beam passing through the radiometer closely resembles the second angle between; so that the height of the matter surface, the radiation source, a function of the radiometer and the cabin spatial relationship is determined as.

The level of substance surface height is generally the first and using the same case of second angles. determines.

Irradiation is typically a 3 mm pen diameter pen. beam carried out using For angular measurement for other purposes such as positioning and/or load control. It is also advantageous to use a corresponding light sensor. Optical reflected light specifically for optical angle measurement especially when using a light sensor, water sources of error must be considered: first, all There are surface waves that cause it to be reflected in the directions it could be. This will cause false signals throughout the range. can open. Therefore, the detection is the peak of the surface waves. long enough on the points or over the area implementation is advantageous. In this way, the maximum signal greatly depends on the angle of reflection of a largely smooth surface. surface by obtaining a corresponding reflection angle waves are averaged. Also from walls and container It is preferable to filter out reflections from the base otherwise however, they may falsify the measurement. When the shape of the container is known, this it becomes possible. In addition, preferably certain liquid whose level is examined It is preferable to consider adhesive strengths based on is done. Adhesive forces are lens-like on the walls inside the container. causes surface formation. Ignoring this surface This may cause errors in the case of narrow containers. can open. Point to consider adhesive forces significantly in size under the inner vessel cross-section is advantageous. In this case, the angle of reflection is horizontal. can be measured as a function of position. Adhesive addictive surface curvature by comparing with a model that takes into account the forces can be determined and compensated. As an alternaive if the point is small enough, the point in question is to the center of the container where the surface is substantially horizontal can be directed. For this purpose, the dot area should be It is preferred that the cross-section be less than one-tenth. a cabin cabin, in order to determine its nature and/or capacity. a mark attached to it or to the container chamber can be read. For example, the sign in question is the one shown in Figure 12. projections can be in the form (721, 722). This sign is moving a light sensor or a light beam or some other can be read through sensors.

As an additional example, the size of the door may be based on size. In this case, the cabin clutch cup size during closing of a clutch lever can be determined by measuring the magnitude of the movement.

As an additional example, the container size depends on the container length. it could be. In this case, the cabinet is upright along its length. be grasped by a grasper in such a way as to be held and one or more of the gripper in a horizontal beam path a defined number of light beams (852) (see Figure la) It is advantageous to move at height. cap length and light based on the size of a particular light beam the beam path of the beam may be broken by the container or may not be included. Length, limitable or light breaking the beam or which beam of light is broken can be determined from the information on determine the length If this information is necessary for on beams of different heights or beams of different heights. can be moved once. The gripper is also on the light beam can be positioned and vertically oriented until the optical path breaks. can be moved.

User Interface, Working Concept The operating concept of the apparatus is simple and intuitive versus is directed. The user's manual of the apparatus specifically contributes to the concept. Apparatus as little input as possible stage will be necessary, the said stages will be possible It will be as intuitive as it is and the user needs where information can be understood as easily as possible. It is designed to be in shape. For example, frequently used the ability to save the settings of the procedures, directly input and rarely require modification. This situation is contributed by omitting the fields.

The apparatus is basically using a TFT touch screen. is run. For example, a screen with a resolution of 240 x 320 pixels. touch screen can be used. Like keys and keys e.g. emergency shutdown function or stand-alone further processing for independent operation of modules The use of elements is also possible optionally. Promise the apparatus in addition to transmitting diagnostic data, software to perform updates or remotely protocols and digital data to load or save parameters The exchange contains interfaces. This and similar in Figure l6 a USB interface (870c) and an R8232 interface for all purpose (870d) is shown.

Typical for the user's guide shown on the screen (910) The screen design is shown in Figure 17b. shown on the screen (930) a title (932), a value field and another miscellaneous key (“standard” (937); “save”; cancel”) is shown.

Title (932), For example, in a user's guide such as menu acts as a redirect to the current location. in the title (932) Extremely long texts have been shortened. This situation is a series of indicated by a dot. The importance of the left or right side of the text The text in question is cut from the left or right, depending on

The Value field is used to change values and value in this case is the buffer unit. An on/off element (938). Press the “+” key of the control element (938) While the corresponding value is increased by pressing the "-" button, the value is increased. is dropped. Possible threshold values (934, 935) are displayed. A When the threshold value is reached, the control element (938) key becomes inactive.

From a fixed number of elements, similar to the value field shown for selecting an element; For example, from several protocols a list field (not shown) to select a ` protocol hosting is possible. To the control element (938) in question similarly for selecting the desired element in the list field. Direction keys are available. Any existing element in bold letters and larger font in the frame is displayed. List field additionally with selected element contains an action key describing the action to be performed.

When the representation of the selected list element is pressed directly, the possible action is taken.

When the apparatus is used for the first time, it is shown in Figure 17a. the initial screen (920) appears. The initial screen in question (920) is fully integrated into the screen described in Figure 17b above. unlike. Two quick-start keys (924), protocol keys (922) and access to equipment settings and other functions a tool key 926 for providing Beginning When the key is pressed on the screen, the user enters the relevant menu. is directed.

Quick start keys (924) last started procedure (left key) and shows the next (right-click) procedure from the last started.

More than two quick-start keys can be offered. Fast Caption of the start keys (924), a protocol changes when started. A speed of a protocol When the allocation of the start key is necessary, the corresponding quick The start key (924) can be operated for a long time. Relating to The independent displayed parameters of the protocols are subject to change. can show. A set of coordinated parameters protocol can be saved and then a list field is selected using In this way, the parameter set is can be adjusted using the options key. For example, in Figures 18a and l8b, for the operation of a protocol Typical operating procedure is shown. First screen (920) It is the initial screen shown in Figure 17a. quick start When the screen (911) is pressed, the apparatus is filled with consumables. instructions for downloading (946) and corresponding protocol one or more containing other preparations for screen 940 appears.

The screen 940 shown is of the reservoir 710 for the buffer fluids. protocol specified for its various locations (942) Shows the required fluids and fluid levels (944) for

Figure l8a shows an overview screen 940.

Here, the fluids are color-coded and the required fluid levels are charted. is displayed as. Detailed information about the installation, is skipped.

When the centrifuge chamber is opened, the user interface also instructions for loading the centrifuge (not shown) displays.

In the example shown, the “skip” button is pressed (911). in Figure 18a. consecutive third screen (950) protocol to be performed Shows an overview of the stages. related stages, are displayed as elements of the progress bar (951).

The following protocol stages are shown on the screen shown: initiation, lysis, incubation, lysis, binding. lack of space other stages that cannot be displayed due to when displaying or with elements such as dots (not shown), additional stages are added to the element. indicated by tapping. buffer type and amount, double illusion, initial volume, RNAse name etc. variable parameters can be adapted. Start Pressing the key (911) causes the protocol to start.

The screens (950) displayed while processing the protocol are in Figure 18b. is displayed. This is the remaining calculated time (954) and actually operated. a `protocol step (952) in a progress screen shows. The protocol in question can be stopped at any time, but cannot be resumed. Cut key, emergency stop button is not. Various processes before the final program end terminated and the shafts return to their starting position.

In addition, the centrifuge and the shaker themselves can be run independently of protocols. to these functions for example the tools menu of the start screen in Figure l7a (926) or using a special key accessible. Various equipment settings are displayed on the initial screen (see Figure 17a). can be selected after pressing the tools button (926). Next a menu containing the following setting options is displayed. use of the centrifuge 200 regardless of protocols; heating and/or agitation regardless of protocols; maintenance, eg resetting moving parts; centrifugal opening or closing the lid; settings, eg display brightness, audio signals; system check, eg daily files, equipment characteristics (serial number, specification etc.) save, playback updates; and eg temperature, voltage, error messages etc. system The order of menu items may be based on frequency of use: how often a function is needed It appears in the menu (up to 3 in the upper section. Basically “Standard”) button is also available. This key is for a selected element. sets standard values.

A USB configured as a USB host on the apparatus interface (870c) is available. This data is from a USB memory can be read or written to memory. Hence the USB memory It acts as a medium between the PC and the apparatus.

Alternatively another interface such as Ethernet or R8232 can be used and other functions such as Web server are also available. realizable.

The following data can be stored with USB memory using USB interface. can be given. protocols* (individual. protocols or several protocols simultaneous loading and saving): equipment information security (e.g. diagnostic information, service data) log files containing or error data, status files etc.) and firmware or software update.

In addition, protocols or other files can be deleted. (independent protocols or several simultaneous protocol). Protocols are preserved as SPS algorithm and xml saved as files.

Any type of file (e.g. to be uploaded or saved) protocols, firmware, etc.) is related to its structure, and the index is the predetermined name. shows. File transfer to or from USB memory using the special utility that provides the menu is performed. User pre-existing on target equipment overwrite files or simply do not pre-exist It has the option to transfer files.

With the R5232 interface equipped with the special service software of the apparatus (870d) other service if connected to a PC functions can also be performed. The operation is the easiest to use for the most common applications. is designed to offer. These are usually new running and deleting protocols.

Protocols In an apparatus with partial modules described above during processing of biological material partial steps that can be realized will be explained by way of example with reference to a protocol. First, as shown in Figure 18a, a desired the protocol is examined and optionally the protocol parameters is selected. Then the apparatus is intended for loading the apparatus in question. gives instructions 940. Apparatus according to the said instructions manually loaded. Alternatively, automatically or partially It can also be loaded automatically. Especially, consumables station (701) (see Figure 12) pipette loaded with hoppers. centrifuge (200) with suitable cup holders (120) and cups (160, 170) is loaded; warmer/shaker (600) samples loaded with containers (170). The samples to be treated are included in the sample cups (170).

Alternatively, loading, for example a station ready for the apparatus (701) or by placing a container carrier (610) realizable.

The loading is then checked for consistency and completeness by the apparatus. is controlled for. As explained earlier, the word The subject control is for example on the work platform and/or on a light sensor movable via ultrasound unit (830) It is carried out through (810). other process parameters (e.g. detected sample cups (170) from the number of samples to be processed) from the control can be done. Then the protocol steps are to process the samples. performed for. By way of example, in cells lysing, pelleting, binding, of a protocol that includes the washing and separation steps. automatic processing is explained. Processing, for example “QIAGEN Bench Guide”, “QIAGEN Guide to Template Purification adn DNA Sequencing” with “QIAGEN QIAprep® Miniprep Handbook” (Second Edition, June 2005) It may also contain other protocols. additionally the protocol stages that have been carried out only some, or in addition to or in place of them may include other stages.

For the lysis stage, the sample material, with the sample cup, optionally with gripper (400) or pipette unit (500) It is transferred manually to the heater/shaker via the

The buffer liquid is pipetted into the sample material. same pipette tip, for example during the pipetting process of the pipette tip in question at a sufficient height to ensure that it is not contaminated It can be used for all samples provided that it is present.

Buffer and sample material warmer/shaker stirred by shaking. Sample as optional a heating method defined in the heater/shaker brought to temperature and for a certain period of time kept at temperature (incubation). Optional other bumper liquids are added. Desired biomolecules residual buffer liquid soluble (lysate).

The lysate is then prepared as described with reference to Figure 7. transferred to the centrifuge by pipetting. to each sample A separate pipette tip is used for Then optionally, the pelleting step is performed.

At this stage, the sample better removes cell debris with the lysate. centrifuged to ensure separation. Sample, transferred to a container in the centrifuge by pipetting. the container in question for example an Eppendorf container (170) held in the hold position (140) or a container 150 integrated into a container holder 120 (eg Figure llc). Alternatively, the sample containing the sample the container 170 is a holding with the gripper 400 in the centrifuge. can be transferred to position 140. Then the sample, cell such that debris will collect mainly on the container floor. centrifuged in the centrifuge (200). Centrifugal centrifuge cover (240) during operation, with cover control (260) is closed. Then access the inside of the centrifuge. reopens to allow provisioning.

Then the binding phase takes place. At this stage, the lysate by pipetting into a filter cabinet 160 or a membrane It is transferred to a container 160 containing carrier material such as

Lysatine, basal portion with increased cell debris not transferred.

The container 160 containing the carrier material is attached to the container holder 120 is in the holding position (130) (or 150). With the centrifuge process biomolecules as the lysate is forced through the carrier material preferably remaining in the carrier material. Remaining lysate, cap it is rinsed towards the waste part (122) of the carrier (120) (see Fig.

Figure llc).

The washing step is then carried out: one or more a large number of washing liquids by using the pipette unit (500). After adding (160), the container (160) being forced through the material or through the carrier material. subjected to centrifugation. These unwanted components removes it from the carrier material. containing solutes washer fluid into the waste section (122) of the cup holder (120) rinsed. Washing stages can be repeated optionally.

The container 160 is transferred to prepare the separation step.

Generally independent of 'and other' protocol stages container to receive biological material, which is primarily a liquid (160) using the grabber prior to the parsing step. other. transferred to a container (170). Preferably. cap, a gripper It is transmitted using (400). Preferably in the grip Fig. 6, 8 and/or some of the features described in accordance with 9. Particularly preferably, the additional container 170 is placed in a centrifuge 200. arranged and most preferably the additional container 170 is placed in a centrifuge. removable in the holding position (140) of the cup holder (120) arranged in a row. Also, in the container (160), the liquid will come out. the presence of an outlet hole for the transmission of all kinds of liquids passing through the outlet hole of the cabinet (160). the relevant cabin so that it will be collected in the additional container (170) after (160) depending on additional container (170) after transfer arrangement is preferred.

It will be transmitted in an unlimited capacity from this chapter onwards. the respective cabinet (160) will be arranged in the first holding position (130). and the additional cabinet (170) cup holder (120) in the centrifuge. arranged in the second holding position 130 (see Figure llc) is assumed. Then, independent of other phases of the protocol container transfer to another container with the gripper as follows can be performed correctly, for example: Fig. in 8 and 9 The gripper unit (400), as shown in the description, It is positioned as attached to its holder (120), the positioning element 452, with the positioning counter element of the cup holder (124) interacts. If necessary, gripper (410), drive rotated by element 431 about axis 436a and so that the gripper can grip the container 160. Kabi grasps and removes it from the holding position (130). Gripper with drive element 431 is rotated about axis 436a. As a result gripper, inserting the container 160 into the additional container (170). relating to releases the container. Positioning throughout this movement with the positioning counter member of the cup holder (452) 124 is preferred to interact with. For example, spring (458) can be guaranteed.

Alternatively, the container 160 may additionally be placed in the first container holder. (120) from the first holding position (130) to an additional container (170) can be transferred, the additional container is located in the centrifuge and in another cup holder different from the first cup holder 120 is arranged. Then, between removal and insertion, additional an additional gripping unit in relation to the cup holder the positioning step must be performed.

Additional action of the gripper 410 and the above-mentioned movement details are described in reference to Figures 8 and 9.

For the separation stage, the separation liquid, coarse 160 (pipeted) is added and vessel 160 is centrifuged.

Separating fluid, desired component other than carrier material dissolves it and washes it into an additional bowl. The component requested here, ready It is retained and dissolves in the decomposition liquid.

As a result, for the final “cleaning” process, intermediate stages of the procedure. e.g. containers used for It can be taken to the waste disposal station (740) manually or automatically. as destroyed.

Claims (1)

REQUESTS 1. For biological material processing, - with a radiation source (810b) for a container for receiving biological material (160, characteristic; cabin with at least one container part of increased recognizability; differential, for illuminating the recording area associated with the container part of increasing recognizability); - with a sensor (810a) for Measuring the radiation intensity from the recording area; - associated with an evaluation unit equipped to record the presence or absence of vessel by comparing the measured intensity with the defined threshold, said sensor (810a) being mobile and the sensor (810a) its position is determined by illuminating the gauge mark on the apparatus with a radiation source (810b) and measuring the radiation from the gauge, the radiation source and the sensor being able to act together, and said apparatus further comprising positioning means for positioning said radiation source and the sensor associated with a holding area. .I It is an apparatus according to stem 1, its feature is; the holder 120 is configured to hold a centrifuge chamber 160, 170, a filter vessel 160, a collecting vessel 170, or a pipette tip 730. Apparatus according to one of Claim 1 or Z, its feature is; configuring said radiation source (810b) to illuminate a membrane, a filter, a filter membrane, a lid hinge, and/or a container lid. Apparatus according to one of claims 1 to 3, characterized in that; The evaluation unit is equipped to record the absence of density measured below the threshold and to record the presence of density measured above equal. The apparatus according to any of the previous claims, and its feature is; whose position is predefined and known to the apparatus. of the sensor in question. is to be configured to move on the setting mark. The apparatus according to any of the previous claims, and its feature is; is that the setting mark is a blocking element for the light beam.