TH9686C3 - Process for producing extracts from allergens from grass pollen. - Google Patents

Abstract

DC60 (18/11/57) Production process of grass pollen allergen extract. For use as a reagent Standard allergy Used in testing and evaluating allergic conditions And used as a component of Grass pollen allergy vaccine Used in the treatment of patients allergic to grass pollen. It consists of the following steps: grass pollen and reeds per solvent (Phosphate buffer saline, PBS) in an appropriate ratio are passed through high frequency sound waves (Sonicate) to be disintegrated and centrifuged for Separate grass and reed sediments. Then extract the fat layer with ether. Will extract allergens from Grass pollen and reeds The extract obtained was measured by the Kjeldahl method to find the protein content in PNU / ml units and then tested for sterility (Sterility test). The colored bottles are stored at 4 ° C. When used, they are added with a PBS buffer solution (PBS) with or without phenol (Phenol). grass For preparation Allergy standard used for testing and evaluating allergic conditions and as a component of Grass pollen allergy vaccine used to treat patients allergic to grass pollen. It consists of the following steps: use of grass pollen. And reed per solvent (Phosphate buffer saline, PBS) in the appropriate ratio, passed through high frequency sound waves (Sonicate) to break down, then centrifuged to separate sediments, grass and reeds. Then extract the fat layer with ether. Will extract allergens from Grass pollen and reeds The extract obtained was measured by the Kjeldahl method to find the protein content in PNU / ml units and then tested for sterility (Sterility test). The white bottle is stored at a temperature of 4 ° C. When used, it is added to PBS buffer. (PBS) that is mixed or not mixed with phenol (Phenol)

Claims (1)

1. The production process of extracting allergens from grass pollen. For the preparation of allergens Standard used to test and assess allergic conditions and as a component of vaccines. Allergy to grass pollen used in the treatment of patients allergic to grass pollen. Consists of the steps of Extraction of allergens from grass pollen. Then the protein content is analyzed. In this crude extract And then taken to collect Or prepared as a standard allergen used in Test and assess allergy conditions And / or used as a component of the pollen allergy vaccine There are special characteristics of the grass used in the treatment of patients allergic to grass pollen. Grass pollen and reed were applied to the solvent (Phosphate buffer saline, PBS) at the appropriate ratio. It was then passed through high frequency sound waves (Sonicate) 130 watt Ultrasonic Processor with Timer and Pulse 20 KHz for 30 minutes using Amplitude 40-454 Pulse-ON for 30 seconds alternately Pulse -OFF for 30 minutes. To centrifuge with a blender Ultrasonic centrifuge at 9,000 rpm (rpm) for 30 minutes to separate the sediment, pollen of grass and reeds, and then extract the fat layer with ether (Ether) in the ratio 1: 1 will get allergen extracts from pollen of grass and reed