TH8735B - Vaccine - Google Patents

Abstract

We are given weakened microorganisms containing two mutated genes, each located in the aromatic pathway of the microorganism. These microorganisms can be utilized in the form of vaccines. They can be genetically engineered to express antigens from other pathogens, and that is the basis of many valuable vaccines.

Claims (15)

1. A method for producing attenuated bacteria involves inserting a second irreversible mutation into the second aro gene in bacteria with the first irreversible mutation in the first aro gene. 2. The method claimed in claim 1, by second mutation, is achieved through transposon mutation. 3. The method claimed in claim 1 involves cloning a second aro gene into a vector, incorporating selected marker genes into the cloned gene to inactivate it, inserting a plasticizer containing the inactivated gene and various selected marker genes into bacteria, and selecting a bacterial mutant where the inactivated gene is recombined into the bacterial chromosome. 4. The method claimed under any one of the claims 1-3, where one of the parties is Chinese aro A. 5. Method according to claim 4, where the second China aro is China aro C or aro E. 6. The method claimed in any of the preceding claims whereby the bacteria are selected from the genera Salmonella, Bordetella, and Haemophilus. 7. The method claimed in claim 6 by the bacterium S. typhi. 8. The method claimed in claim 7, by the bacterium S. typhi strain Ty 2. 9. The method claimed in any of the preceding claims whereby the generated weakened bacteria undergo irreversible mutation in each of the two separate aromatic biosynthetic genes is accomplished by providing a characterization cassette containing the DNA sequence encoding the pathogen's antigen. 10. The method claimed in any of the preceding claims, whereby the generated attenuated bacteria undergo irreversible mutation in each of the two separate aromatic biosynthetic pathways, is co-added with a pharmaceutically recognized carrier. 11. A method for constructing a vaccine consisting of the addition of two separate, irreversible mutated Chinese aro bacteria, each in its respective aromatic biosynthesis pathway, to a pharmaceutically recognized carrier. 12. Method according to claim 11 by one of the aro entities is Chinese aro A. 13. Method according to claim 11 by Chan aro second is China aro C or aro E. 14. Any method under any of the claims 11 through 13 where the bacteria are selected from the genera Salmonella, bordetella, and Haemophilus. 15. The method under claim 14 by the bacterium S. typhi (Clause 15, 2 pages, 1 image).