TH69235B - Corn stalks and kernels that are fortified for aspartame and protein - Google Patents

Abstract

DC60 (14/07/49) This invention involved corn plants and kernels that were fortified with levels of proteins and amino acids. The invention also involved DNA building blocks, which provided expression in corn cells that were It was reprocessed of asparagene synthase, while DNA was used in a method to produce plant and Corn kernels were reprocessed and selected for plants and kernels with enhanced levels of protein and amino acids. The invention involved plants and corn kernels that were enriched with protein and amino acid levels. Involved in the DNA building block, which provides expression in corn cells that It was reprocessed of asparagene synthase, while DNA was used in a method to produce plant and Corn kernels were reprocessed and selected for plants and kernels with enhanced levels of protein and amino acids.

Claims (2)

Disclaimer (all) which will not appear on the advertisement page. : ------ 06/07/2018 ------ (OCR) page 1 of the number 2 pages Clause 1. and (c) the nucleic sequence which is mated to the sequence of ( a) or (b) or add-on to this section under high-rigidity conditions 0.2 X SSC and 65? h 1. 4. Method of claim 1 which is further incorporated into the procedure f ) Selection of kernels containing proteins that were increased by 1.and (c) the nucleic sequence which was bred to the sequence of (a) or (b) or its complement under the condition of high 0.2 intensity. X SSC and 65 ° C 1. 1. 1. 9. Corn-based feed of claim 5 or 6 ------------ Revised 15 g. Nov. 2017 1. and (c) nucleic acid sequences which are mated to the sequences of (a) or (b) or complement to this under High rigidity conditions 0.2 x SSC and 65 ํ --------------------------------------- ----------- (Old) 1. Method of selection of bred corn stalks with added asparagene that contains a) corn cell transformation that has an integral part. Generates heterogeneous DNA made up of promoter molecules. Where the DNA molecule conveys the polypeptide code of asparagene synthase and b) the ingestion of corn cells and conventional corn plants; And c) selection of the above. Which contains asparagene added to tissues relative to Untransformed corn stalk tissue with such DNA generators; And d) full maturation of the corn plant; And e) the harvesting of the kernels from the corn plant 2. Method of claim 1 where the Molecule DNA was selected from the group containing the specific number 1, 3, 5, 7, 9, 10, 11. , 12, 13, 14, 15, 16 and 17 1. 4, 6 and 8. 4. Method of selection of maize that was bred with protein added. Consisting of a) the transformation of corn cells that make up heterologous DNA composed of a promoter molecule. Where the binding of the DNA molecule conveys the polypeptide code of the asparagene synthase of corn; And b) traditional maize and plant cell culture; And c) Selection of corn plants Which contains asparagene added to tissues relative to Corn stalk tissue that was not transformed with the said DNA generator; And d) full cultivation of corn plants; And e) harvesting the seed from the corn plant; And f) selection of the protein-added seed 5. Method of claim 4, whereby the DNA molecule is selected from the group. It consists of a specific number No. 1, 3, 5, 7, 9, 10, 11, 12, 13, 14, 15, 16 and 17. 6. Method of claim 4, whereby the polypeptide of the AS. Paragene Synthase was selected from a group containing the sequence number: 2, 4, 6, and 8. 7. A corn meal containing protein was added relative to other corn-based foods, where Corn meal Contains a heterochromatic DNA building block consisting of promoter molecules. Where the binding of the DNA molecule conveys the polypeptide code of the asparagene synthase of corn; And 8. Corn meal of claim 7 whereby corn aspartate synthase enzyme is the AsnS 2 polypeptide of the maize. 9. Corn meal of the article Holds 7, where the DNA molecule is the prime number sequence: 3 1 0. Corn meal of claim 7 where the DNA molecule conveys the polypeptide code of the prime number sequence: 4 1. 1. Corn kernels containing the enzyme aspartame. Syntheses are produced by the expression of heterologous DNA builders. That constitutes the DNA molecule that conveys the Poe code Lypeptide of asparagin synthase 1 2. Corn meal is made from corn kernels containing asparagene synthase produced by the expression of heterologas DNA building blocks. Containing DNA molecules that convey the polypeptide code of asparagene, synthase of corn.