TH63314B - Antifreeze protein preparation - Google Patents

Abstract

DC60 (30/01/47) A method for enhancing the characterization of type III antifreeze protein when the protein is prepared by the heterologous (species) fungal (Fungal) transmission of To code the protein sequence gene, by means of Reduce the size of glycosylation The glycosylation of the protein was added to a method for enhancing the specific properties of the type III antifreeze protein when the protein was prepared by the heterologous (species) fungal (Fungal) transcription of the coding. Protein sequence genes, by means of Reduce the size of glycosylation (glycosylation) of protein down

Claims (1)

Disclaimer (all) which will not appear on the announcement page: EDIT 10/04/2017 1. Method for producing anti-coagulation protein lll (AFP) where such method It consists of indifferent expression within the fungal host cell. Cozylation of AFP lll protein compared to parent strains And the nucleic acid sequence coded For the AFP protein 2. Method according to claim 1, where fungal host cells were free from protein glycosylation by Causes a mutation of one or more genes that are coded for the associated enzyme. And protein glycosylation 3. Method of claim 1 or 2 where fungal cells are free from O-Glycosylation 4. Method of one of the above claims in which fungal cells are free from manno protein activity. At least one or more types of syltranferase (PMT) enzymes 5. Method of one of the above claims where the fungal cell is yeast 6. Method according to claim 5 Where the yeast is a mutant without PMT 1. 7. Method of claim 5 or 6, where the yeast Mutants without PMT 2 8. One of the Claims 5 to 7 method. Where the yeast Is Zaccaromycin Cerevice 9. Method of one of the above claims where AFP lll is type lll HPLC-12 1 0. Composition where Contains recombinant, antifreeze protein lll, where 50 to 99 percent of AFP is free of glycosylate 1 1. Composition according to claim 10, where AFP lll This is the type lll HP LC-12 ------- 1. A method for producing anti-cryogenic protein lll (AFP) where the method consists of Indoctrination Fungal host cells were devoid of glycovidation of AFP lll protein compared to parent strains. And the nucleic acid sequence that defines the code for the protein 2. The method is consistent with claim 1. Here, fungal host cells, or fungal, are deficient in glycosylation protein, with good conversion properties. Enzymes code one or more genes combined. 3. The method is consistent with claim 1 or claim 2. Here, the fungal cell is deficient in O-glycosylation. One of the preceding fungal cells here is lacking in the manno protein reaction. Synthesis (pmt) enzyme one or more 5. The method conforms to one of the previous claims in Here, the shell fungal (fungal) is yeast 6. Method complies with claim 5. Here, the yeast is the deficient modified strains -pmt1 7. The method conforms to claim 5 or claim 6. Is the Strain Transfigured - pmt2 8. Method Corresponds to one of the 5 to 7 Claims Here, the yeast is Saocharomyces cerevisiae 9. The Method corresponds to one of the previous claims here. Type III AFP is HPLC-12 Type III 1 0. The composition includes The recombinant type III antifreeze (AFP) protein here, from about 50 percent to 99 percent of AFP, is glycosylated 1. 1.Element complies with claim 10, hereinafter Type III AFP is HPLC-12 Type III.