TH61978B - Methods and equipment for identifying points And separating blood cells without enveloping fluid - Google Patents

Abstract

DC60 (06/06/60) This invention involves a method And a device that uses a method of identification, identification and classification of blood cells Which can be applied appropriately with But without being limited to the counting and classification of white blood cells. It is also especially relevant to Knowledge circle of normalized hematological instruments with moderate operating costs The fabricated method is characterized by the impedance measurement technique used. For identifying particles Which has a trajectory that does not pass through a predetermined optical measuring region in order to specifically process these particles. Which is to avoid Encapsulated fluids are used to guide particles to the measuring region. This invention involves a method And a device that uses a method of identification, identification and classification of blood cells Which can be applied appropriately with But without being limited to the counting and classification of white blood cells. It is also especially relevant to The knowledge circle of normalized hematological instruments with moderate operating costs, the invention-based methodology, was characterized in such a way that the impedance measurement technique. Will be used for particle identification Whose trajectory does not pass through the measuring area Predetermined In order to specifically process all of these particles, in order to avoid the use of enveloping fluid for directing them to the measuring zone,

Claims (1)

Disclaimer (all) which will not appear on the advertisement page: Revised 6/6/2017 1. Method of identification. And separating blood cells without enveloping fluid For particle characterization, it is composed of steps of: fluid transfer (3) with particle (4) through the microparticle (1) of the cell - of flow through the opening (2), where the cell - of the flow through this opening, at least partially transparent, with Significant to at least one wavelength of interest, and at least partially dielectric. Significantly, a measure of the electrical impedance variation of the particles produced by displacement. Through the particles (4) contained in the fluid (3) through the microscopic holes (1) of the cells - of the flow through the opening (2) using an electrode (5) placed on the side. In the opposite direction of the cell - of the flow through the aforementioned opening (2) to establish a longitudinal electric field through the aforementioned holes (1) and to obtain a transformation. Of the electrical impedance of the particle in the form of pulses (41) where the shape of the pulses is dependent on The trajectory of particles (4) within the small holes (1) due to the irregular geometric characteristics. Of the electric field lines, a measure of at least one optical property of such particles (4), which interacts with At least one (7) beam that passes through the microscopic hole (1) of the cell-flow through the opening (2), characterizing the particle (4) by using such measurements of variation. of Particle electrical impedance And optical properties of that particle, inferencing information relating to the trajectory of the particle (4) within the microscopic hole (1) of the cell-flow through the opening (2), at least from the analysis of the shape Of pulses (41) of such measurements of the variations in electrical impedance, the use of such information in relation to the trajectory, at least for the use of Benefits of such measurements of optical properties during particle characterization All of them, including The rejection of the measurement corresponds to the particle (4) whose trajectory is outside the predetermined reliable optical measurement zone (32) relative to the indication characteristic to define the limit in the pulses shape. The flow (41) is, where only the fluid (3) with the particles (4) is made through the cell - the flow through the opening (2) only (original). 1. a method of identifying points And to classify blood cells for the characterization of particles comprising different stages of - fluid transfer (3) with particles (4) through the cells - of flow through openings (2) where cells - of The flow through these openings, at least partially, is significantly transparent to the At least 1 wavelength of interest And at the very least, there is a significant part of the dielectric - a measure of the electrical impedance variations arising from the passage of such particles (4) present in the fluid (3 ) Through cells - of flow through openings (2) - measurement of at least 1 optical properties of such particles (4) interacting with At least one (7) beam of light that passes through the cell - of flow through the opening - characterizes such particles (4) by using such measurements of variation. Of electrical impedance And optical properties It is characterized in such a way that it also includes the steps of - inferencing information related to the trajectory of particles (4) in the cell - of the flow through the opening (2). From the analysis of such measurements of variation Of electrical impedance - use of such information concerning the trajectory, at least for the use of The benefits of such measurements of optical properties during characterization of particles are