TH54852A - Method for producing L-lysine - Google Patents

Abstract

Corine forms bacteria with high L-lysine production capacity. Acquired by combining genes Which provides code for aspartokinase That originate from the chorine form bacteria that impede feedback by L-lysine. And L-threonine The agility was reduced to the chromosomaldNA of the corine form bacteria that are home to the leaky homocerene dehydrogenase or the deficient coryonform bacteria. Homoserene dehydrogenase gene

Claims (9)

1.DNA parts That gives the code for the homoserine Dehydrogenase that started from Corine forms bacteria in which the amino acid oxidant corresponds to the 23rd amino acid resin of the ID number sequence: 4, as measured from the N- tip, is an amino resin. Except leucine or amino acid resin, which corresponds to the 104th amino resin of the sequence ID: 4, as measured from the N- tip, is the amino resin, except Leucine or amino acid resin that matches the 23rd amino acid resin of the ID number sequence: 4, as measured from the N- tip, is amino resin, except leucine. And the amino resin acid that corresponds to the 104th amino acid resin of the sequence ID No. 4, as measured from the N- end, is the amino resin except valine. 2. Corine-form bacteria, which are home to genes, are coded homoseri dehydrogenases, a mutant type that originates from the chorine form bacteria, where the amino acid resides. That is, the 23th amino acid resin of the sequence ID number: 4, as measured from the N- end, is the amino resin, except leucine or the amino acid resin at Corresponds to the 104th amino acid resin of the ID number sequence: 4, as measured from the N- tip, is the amino resin, except leucine or amino acid resin that matches. The 23rd amino acid resin of the ID sequence ID: 4, as measured from the N- tip, is amino acid resin, except leucine and amino acid resin that match the amino acids. The 104th minoresidue of the ID number sequence: 4, as measured from the N-tip, is amino acid, except valine. 3. Corine form bacteria as described in claim 2, which is transfused by combining the genes coded for homoserine. Mutant type dehydrogenase into chromosomal DNA by homologous method Recombination with Homorene Dehydrogenase gene on chromosomes of corine to form bacteria. 4. Corine form bacteria as stated in Clause 2, where the amino acid acid corresponding to the 23rd amino acid resin of the ID number 4 sequence as measured by The N-tip is a phenylalanine or amino acid oxidant that corresponds to the 104th amino acid resin of the ID number sequence 4, as measured from the N- tip, is isoleucine. Or amino acid resin that corresponds to the 23rd amino acid resin of the ID number 4 sequence as measured from the N- end, namely phenylalanine and amino resin. That corresponds to the 104th amino acid resin of the sequence ID number: 4, as measured from the N- tip, is isoleucine. 5. Corine forms bacteria, which are home to genes, coded homoserine. Its gene dehydrogenase is destroyed by combining DNA fragments coded for part of the homoserine. Dehydrogenase initiated from corine to form bacteria into chromosomaldNA by homologous recombination with homoserene dehydration. Drogenase genes on chromosomes of corine form bacteria. 6. Corine form bacteria, which are home to recombinant DNA in their cells, are formed by binding the espartokinase gene that originates from the corine form. Bacteria and wacters are self-replicating in the cells of the corine form bacteria, where their homorene dehydrogenase genes are destroyed by combining the DNA fragments that are coded for. Part of the homosyrine Dehydrogenase that starts from corine form Bacteria enter their chromosomes by homologous methods. Recombination with homoserene Dehydrogenase gene on chromosomes 7. Corine form bacteria as described in Clause 6, where the aspartokinase gene is the genes for aspartokinase. Which prevents feedback By L-lysine and L-threonine in it Has been greatly reduced its agility 8. Corine form bacteria as described in claim 6, where aspartokinase and inhibition of L-lysine reactivity are reduced. And L-threonine is the mutant type of aspartokinase, where the amino acid oxidant corresponds to the 279th amino acid resin of the ID number sequence. Where: 9, as measured from the N- tip, is amino acid resin, except for alanine or the amino acid resin, and the amino acid resin that matches the amino acid. The 30th minoresidew of the sequence ID number: 11, as measured from the N- tip, is amino acid resin, except for alanine or resinic amino acids. 9. Corine forms bacteria which are transformed by incorporating into chromosomal. DNA with a gene coded for aspartokinase that originates from the corine form. Bacteria which impede feedback By L-lysine and L-threonine Where homosyrine Its gene dehydrogenase is destroyed by combining DNA fragments coded for part of the homoserine. Dehydrogenase originates from corine forms bacteria in their chromosomes by homologous method. Recombination 1 0. Corine form bacteria as described in Clause 9, where aspartokinase and inhibition of L-lysine reactivity are reduced. And L-threonine is the mutant type of aspartokinase, where the amino acid oxidant corresponds to the 279th amino acid resin of the ID number sequence. Where: 9, as measured from the N- tip, is amino acid resin, except for alanine or resinic amino acid, except alanine or amino acid residuric acid. Dew and amino acid resin, corresponding to the 30 amino acid resin of the sequence ID number: 11, as measured from the N- tip, are amino resin, except ala. Nine or amino acid residium 1 1. Corine form bacteria, which are home to recombinant DNA in their cells, are formed by binding the espartokinase gene that originates from the corine form. Bacteria and wachers are self-replicating in the bacterial cells of the corine and are transformed by homoserine integration into their chromosomes. Mutant gene dehydrogenase coded homoserene dehydrogenase that originates from the bacterial form of chorine, in which amino acid oxidation is associated with amino acids. The 23rd minoresidew of the ID number sequence: 4, as measured from the N-tip, is amino acid resin, except leucine or amino acid resin that matches the amino acid. The 104th resin of the sequence ID No.: 4, as measured from the N- tip, is amino acid resin, except valine or amino acid resin that matches the amino acid. The 23rd of the sequence ID number: 4, as measured from the N- tip, is the amino acid corresponding to the amino acid. Residu except leucine and amino acid resin, corresponding to the 104th amino acid resin of the ID number sequence: 4, as measured from the N- tip, is an amino resin. Dew except Valine by Homogas Recombination and Homoserine Dehydrogenase genes on chromosomes and corine form such bacteria and therefore do not express homoserine. Traditional dehydrogenase 1 2. Corine form bacteria, as stated in Clause 12, where asparitokinase and its impediment to double feedback greatly reduced agility 1. 3. Corine form bacteria as described in Clause 9, where aspartokinase and inhibition of L-lysine reactivity are reduced. And L-threonine is the mutant type of aspartokinase, where the amino acid oxidant corresponds to the 279th amino acid resin of the ID number sequence. Where: 9, as measured from the N- tip, is amino acid resin, except for alanine or resinic amino acid, except alanine or amino acid residuric acid. Dew and amino acid resin, corresponding to the 30 amino acid resin of the sequence ID number: 11, as measured from the N- tip, are amino resin, except ala. Nine or amino acid residium 1 4. Corine forms bacteria, which are transformed by inclusion into chromosomal. DNA with a gene coded for aspartokinase that originates from the corine form. Bacteria which impede feedback By L-lysine and L-threonine Where homosyrine Its gene dehydrogenase is destroyed by combining DNA fragments coded for part of the homoserine. Dehydrogenase originates from coryene, a bacterial form, where amino acid oxidants match the 23rd amino acid residua of the sequence ID number: 4, as measured by The N-tip is amino acid resin, except leucine or amino acid resin that matches the 104th amino acid resin of the ID number sequence: 4, as measured from the N tip. - is amino resin, except valine or amino acid, which corresponds to the 23th amino acid resin of the ID number sequence: 4, as measured from the N- end. Is an amino acid decidua that corresponds to amino acids Residu except leucine and amino acid resin, corresponding to the 104th amino acid resin of the ID number sequence: 4, as measured from the N- tip, is an amino resin. Dew except Vali by Homogas Recombination with Homosirine Dehydrogenase genes on chromosomes and corine form such bacteria and therefore do not express homoserine. Traditional dehydrogenase 1 5. Corine form bacteria as described in Clause 14, where aspartokinase and inhibition of L-lysine reactivity are reduced. And L-threonine is the mutant type of aspartokinase, where the amino acid oxidant corresponds to the 279th amino acid resin of the ID number sequence. Where: 9, as measured from the N- tip, is amino acid resin, except for alanine or resinic amino acid, except alanine or amino acid residuric acid. Dew and amino acid resin, corresponding to the 30 amino acid resin of the sequence ID number: 11, as measured from the N- tip, are amino resin, except ala. Nine or amino acid residium 1 6. L-lysine production method comprising the process of culturing the coryoform bacteria as described in Clause 2 in appropriate media. Production and accumulation of L-lysine in its culture media And the collection of L-lysine from that culture agent 1 7.DNA parts As set out in Clause 1, where Homosyrine Dehydrogenase has an ID sequence of 4, where the 23 amino acid reside, as measured from the N end - is the amino acid reside except leucine or amino acid. Norexium 104, as measured from tip N - is amino acid reside except valine or 23rd amino acid reside, as measured from N end - is amino acid Aminoresiudew except leucine and the 104th amino acid reside, as measured from the N end - is the amino acid reside except valine 1. 8.DNA parts As stated in Clause 17, where the 23rd amino acid reside, as measured from the N-tip, is phenylalanine or the 104th amino acid reside, as required. Is measured from the N end - is isoleucine or the 23rd amino acid reside, as measured from the N end - is phenylalanine and amino acid reside at 104, as measured from the N tip - is isoleucine 1. 9. Corine form bacteria as described in Clause 2, where homocerene dehydrogenase has an ID number sequence: 4, where amino acid resiudine 23. As measured from tip N - is amino acid reside except leucine or 104th amino acid reside, as measured from tip N - is amino reside except wa. Lean, or the 23rd amino acid resin as measured from the N end - is the amino acid reside except leucine and the 104th amino acid resin as measured from the tip. N - is amino acid reside, except valine 2. 0. Corine form bacteria as stated in Clause 19, where the 23 amino acid reside as measured from the N-tip is phenylalanine or amino acid. Sewdew 104, as measured from the N end - is isoleucine, or amino acid reside 23, as measured from the N tip - is phenylalanine and amino acid. Sew that 104, as it is measured from the N tip - is isoleucine 2. 1. Corine form bacteria as described in Clause 8, where aspartokinase has the alpha subunits - with the sequence ID number: 9 and the beta subunit. Where the amino acid acid corresponding to the 279 amino acid resin of the sequence ID number: 9, as measured from the N- end, is the amino acid. Amino acidic acid except alanine or amino acid resin, except alanine or amino acid is a direct resinic acid and amino acid resin. With amino acid resin, the 30th of the ID number sequence: 11, as measured from the N- tip, is amino acid resin, except for alanine or the amino acid resin. Wor 2 2. Corine form bacteria as stated in Clause 21, where amino acid-corrosive acid matches the 279 amino acid resin of the sequence ID number: 9, as is Measure from the end N- selected from a group containing threonine, arginine, cysteine, phenylalanine, proline, serene, tyrosine and valine and amino acids. The acid that matches the amino acid resin of the 30th sequence ID number: 11, as measured from the tip. N- selected from a group containing threonine, arginine, cysteine, phenylalanine, proline, serene, tyrosine and valine, and 2. 3. Corine form bacteria as described in Clause 10, where aspartokinase has the alpha subunit - with the sequence ID number: 9 and the beta subunit. Where the amino acid acid corresponding to the 279 amino acid resin of the sequence ID number: 9, as measured from the N- end, is the amino acid. Amino acidic acid except alanine or amino acid resin, except alanine or amino acid is a direct resinic acid and amino acid resin. With amino acid resin, the 30th of the ID number sequence: 11, as measured from the N- tip, is amino acid resin, except for alanine or the amino acid resin. Wor 2 4. Corine form bacteria as described in Clause 23, where amino acid-corrosive acid matches the 279 amino acid resin of the sequence ID number: 9 as measured. From the end, N- is amino acid resin, except alanine or amino acid resin, except alanine or amino acid is resin and amino acid. Norezidyu corresponding to the 30th amino acid resin of the sequence ID No.: 11, as measured from the N- end, is amino acid resin except alanine or amino acid. No-acidic resin 2 5. Corine form bacteria as described in Clause 13, where aspartokinase has the alpha subunit - with the sequence ID number: 9 and the beta subunit. Where the amino acid acid corresponding to the 279 amino acid resin of the sequence ID number: 9, as measured from the N- end, is the amino acid. Amino acidic acid except alanine or amino acid resin, except alanine or amino acid is a direct resinic acid and amino acid resin. With amino acid resin, the 30th of the ID number sequence: 11, as measured from the N- tip, is amino acid resin, except for alanine or the amino acid resin. Wor 2 6. Corine form bacteria as stated in Clause 25, where amino acid-corrosive acid matches the 279 amino acid resin of the sequence ID number: 9, as is. Measure from the end N- selected from a group containing threonine, arginine, cysteine, phenylalanine, proline, serene, tyrosine and valine and amino acids. The acid that matches the amino acid resin of the 30th sequence ID number: 11, as measured from the tip. N- selected from a group containing threonine, arginine, cysteine, phenylalanine, proline, serene, tyrosine and valine, and 2. 7. Corine form bacteria as described in Clause 15, where aspartokinase has the alpha-subunit with the sequence ID number 9 and the beta-subunit. There is a sequence of ID number 11, where the amino acid corridor matches the 279 amino acid resin of the ID number sequence: 9, as measured from the N- tip, is the amino acid. Residu except alanine or amino acid resin, except alanine or amino acid is a resinic acid and amino acid acidic acid. The 30th amino resin of the sequence ID No.: 11, as measured from the N- tip, is amino acid resin except for alanine or the amino acid resin, resin 2. 8. Corine form bacteria as described in Clause 26, where amino acid-corrosive acids match the 279 amino acid resin of the sequence ID number: 9, as is. Measure from the end N- selected from a group containing threonine, arginine, cysteine, phenylalanine, proline, serene, tyrosine and valine and amino acids. The acid that matches the amino acid resin of the 30th sequence ID number: 11, as measured from the tip. N- selected from a group containing threonine, arginine, cysteine, phenylalanine, proline, serene, tyrosine and valine, and 2. 9. Corine form bacteria as described in Clause 11, where homocerene dehydrogenase has an ID number sequence: 4, where amino acid reside 23 follows. As measured from the N end - is amino reside except leucine or 104th amino acid reside, as measured from the N end - is amino reside except vali. N, or 23rd amino acid reside as measured from the N end - is the amino acid reside except leucine and the 104th amino acid reside as measured from the N end. - is amino acid reside, except valine 3 0. Corine form bacteria as described in Clause 14, where homocerene dehydrogenase is assigned an ID number: 4, where amino acid reside 23 follows. As measured from the N end - is amino reside except leucine or 104th amino acid reside, as measured from the N end - is amino reside except vali. N, or 23rd amino acid reside as measured from the N end - is the amino acid reside except leucine and the 104th amino acid reside as measured from the N end. - is amino acid reside, except valine