TH52487B - Methods for L-Glutamine production and L-glutamine producing bacteria. - Google Patents

Abstract

DC60 (13/02/47) L-Glutamine was produced by culturing the bacteria Corine form capable of producing L-Glutamine and was modified to provide Intracellular glutaminease function is reduced, and is commonly used, for example, that has been modified to achieve the mentioned intracellular glutamine function. Of the Production includes bacterial cultures in the medium, followed by L-glutamine accumulation in the medium and L-glutamine collection. From that medium L-Glutamine is produced by culturing bacteria that can produce L-Glutamine and is modified to provide The intracellular glutaminease function is reduced, and is commonly used, for example, that has been modified to make glutamine syntase function within the cells mentioned. Of the Production includes bacterial cultures in the medium, followed by L-glutamine accumulation in the medium and L-glutamine collection. From that medium

Claims (1)

1. Corine form bacteria capable of producing L-glutamine and being modified to provide The function of glutamine in the cells is reduced. 2. Bacteria of the claim No. 1 by which glutamine functions Mines within the cells mentioned were Reduced by making the gene Glu The taminase on the chromosome is broken.3.Bacteria of claim 1, where glutamine function is 0.1 U / mg of cell protein or less.4.Bacteria of claim 1. Where acting glutamine The mines within the cells mentioned are similar or less common than doing. Glutamine functions Synthase, when measured as a function of the cellular protein weight unit 5. Bacteria of claim 1, which has been modified to Gives the function of glutamine syntase within the cells mentioned. Make it stand out. 6. Bacteria of claim No. 5, where the action of the mentioned glutamine syntase was performed. To stand out by most of the time Expression of the genes glutamine chinase 7. bacteria of claim 6, with an increase in The expression of the glutamine syntase gene was made. It was obtained by increasing the number of repetitions of the genes transcribing glutamine syntase, or by modifying the expression of the regulator. A gene that decodes glutamisinase, in order to make gene expression The bacteria are more prominent. 8. Methods for L-Glutamine production, consisting of a) Bacterial culture of claim No. 1 in the medium to produce L-Glutamine. And b) L-glutamine from that medium 9. Glutamine Synthase genes derived from corene form bacteria, whereby the region arrangement - 35 genes were replaced by TTGCCA, and the collation of the -10 region of genes was replaced by TATAAT 1 1.1. 1. Glutamine Syntase gene of claim 9, whereby the gene decoding the sequined protein Amino Acids of Arrangement: 4