TH52219C3 - Methods of amino acid-producing bacteria and methods of amino acid-producing bacteria by fermentation with amino acid-producing bacteria created. - Google Patents

Abstract

DC60 (17/12/42) Method of production of corine form bacteria that produce good amino or nucleic acids. Up that consists of steps Of mutagenicity in the arrangement of biosynthetic gene activators, amino acids or nucleic acids on chromosomes. Of corine form to make It's close to the sort. Coherent Or importing changes in the arrangement of biosynthetic genes, amino acids or acids The nucleicides on the chromosomes of the corine form bacteria by recombination of genes to make them closely aligned. Put together to get yourself Mutation of microbial corineforms producing amino acids or nucleic acids, mutagenic culture Breeds and mutants capable of producing large amounts of the intended amino acids or nucleic acids, this method can create mutations that can increase or control the proper expression of the intended gene. Without the use of plasmids And likewise, amino acids can be produced in high results by Reunification or mutation Methods for producing corine form bacteria that produce good amino acids or nucleic acids. Up that consists of steps Of mutagenicity in the arrangement of biosynthetic gene activators, amino acids or nucleic acids on chromosomes. Of corine form to make It's close to the sort. Coherent Or importing changes in the arrangement of biosynthetic genes, amino acids or acids The nucleicides on the chromosomes of the corine form bacteria by recombination of genes to make them closely aligned. Put together to get yourself Mutation of microbial corineforms producing amino acids or nucleic acids, mutagenic culture Species and mutants capable of producing the intended amount of amino acids or nucleic acids, this method can produce mutations that can increase or control the proper expression of the intended gene. Without the use of plasmids And likewise, amino acids can be produced in high results by Reunification or mutation

Claims (7)

1. a method of production of corine form bacteria that results in the production of amino acids or nucleic acids that Better, which consists of The mutation stage in the activation of biosynthetic genes of amino acids or nucleic acids on Chromosomes of the bacteria corine form for Make it close to The corresponding alignment or import changes in Alignment Stimulation of biological synthesis genes, amino acids or Nucleic acids on the chromosomes of cattle bacteria Reformed by returning Combine genes to bring them closer to coherence to achieve microbial mutation. Reneformations that produce amino acids or nucleic acids, cultivate mutants and select mutants that can be produced. Amino acid or the intended amino acid in large quantities 2. Method of claim 1, where amino acids are selected. From the group containing glutamine acid Mix, Lysine, Arginine, Serine, Fe Nylalanine, proline And nucleic acids were selected from the group consisting of iosine, guanosine, acenosine, adenosine and nucleotides 3. Method of claim 1, where Amino acid Tamics and activators for genes Biological synthesis is selected from Group consisting of glutamate dehydrogenase (GDH) gene activators, citrate synthase (CS), isocitrate sitase (JCDH), pyruvate dehydrate. Drogenase (PDH) and Adinase (ACO) 4. Method of claim 3, whereby glucogenase activation Tame dehydrogenase (GHD) has at least one DNA coordination selected from a group consisting of (i) CGGTCA, TTGTCA, TTGACA, and TTGCCA in the -35 domain, (ii) TATAAT or It is sorted like TATAAT, but the ATAAT substance is replaced with another and which is not wrinkled. Withholding one's duty The stimulus in domains-10 and (iii) is the combination of (i) and (ii) 5. Method of claim 4, where the stimulus for GDH has TGGTCA in domain -35, and TATAAT in domain -10. , Or TTGTCA in domain -35, and TATAAT in domain -10 6. The method described in claim 3, where the actuator for CS contains (i) TTGACA collation in domain -35, (ii). The TATAAT arrangement in the -10 domains, or (iii) the conjunction of (i) (iii), the arrangement of (i) and (iii), and this arrangement does not impede. The function of the actuator 7. Method of claim 3, where at least one Of the first and second actuators for ICDH, there are (i) TTGCCA or TTGACA arrangements in the -35 domains, (ii) TATAAT arrangements in the -10 domains, or (iii) joint arrangements of ( i) and (ii), and no collation Interrupt Of actuator 8. Method of claim that 3, where the actuator for PDH has (i) TTGCCA collation in -35 domain, (ii) TATAAT collation in -10 domains, or (iii) conjunction of (i) and ( ii), and the arrangement does not inhibit its function Of activator 9. Method of claim 1, where amino acids are Arginine, and a stimulant for the gene Bio-synthesis for Arginanosaxinesyntase 1 0. Method of claim 9, where the stimulus for Arginino Sud Sinate Syntes Me At least one DNA collation was selected from a group containing (I) TGCCA, TTGCTA and TTGTCA in the -35 domain, (ii) TATAAT or TATAAT collation, but where the ATAAC substance was replaced by another. One that does not impede the function of stimulants in domain-10, and (iii) the combination of (I) and (ii) 1 1. Method of claim 10, whereby the stimulus for Arginino Succinate Syntase At least one DNA collation was selected from a group containing (I) TTGTCA in the -35 domain, (iii) TATTAAT collation in the -10 domains, and (iii) the conjunction of (I) and (iii) 1. 2. Gene that synthesizes glutamic acid with the said stimulant. Included in claims 4 through 8 1 3. Gene that synthesizes arginine with the said activator. In claim 10 1 4. Corine form bacteria that produce glutamic acid with the genes Synthesizes glutamic acid listed in claim 12 1. 5. Corine form bacteria that produce arginine containing the genes Synthetic arginine identified in claim 13 1 6.Methods of producing amino acids or amino acids over the acid Nucleicides by heavy loads where Consists of the seeding process Cultures corine form bacteria that are formed by the methods listed in Claims 1 through 11 and result in the production of amino acids or acids. Improved nucleicides, or cory bacteria This form of claim 14 or 15 in the culture medium and where amino acids or acids are accumulated. The intended nucleic in the medium, and their collection from the media 1 7.Methods of producing L-glutamic curl by fermentation, where Consists of the steps of Cultured Corineform bacteria that produced a built-in 4-fluoroglutamic acid-resistant L-glutamic grind. In the middle of the liquid to create and by which L-glutamic acid accumulates in it Medium and collecting it from the body Culture medium