TH4993A - Decoding of hepatitis B antigen from recombinant baculovirus vector. - Google Patents

Abstract

This invention describes the transcription of hepatitis B virus (HBV) human antigen in cultured insects or insect cells. Acceptable insects or insect cells are infected with recombinant baculovirus containing the human HBV gene inserted into the baculovirus genome under the control of the polyhecrin gene promoter and within the starting sequence of the viral polyhecrin protein.

Claims (8)

1. The process of producing probe peptides containing at least a portion of the HBSAG antigen or pre-S2 protein involves infecting recombinant insects or insect cells with transcription vectors containing recombinant saccharides with the DNA fragment encoding the peptide, under the transcriptional control of the polydrin promoter. 2. Procedure under claim 1, where recombinant baculovirus exhibits polyhecrin-negative characteristics. 3. A procedure under claim 1 or 2 where the recombinant baquilovirus is derived from the ortographa californica nuclear polyhecrolis virus. 4. The process under any of the aforementioned claims, where the decoded vector is obtained from the recombination of a recombinant transfer vector containing the said DNA fragment and the DNA infected with the said saculovirus. 5. The procedure under claim 4 involves infecting accepted insects or insect cells with the recombinant DNA, along with a recombinant transfer vector containing the aforementioned DNA fragment and the DNA infected with the saculovirus. 6. The procedure under any of the above claims, where the said DNA contains the BAM HI inserted fragment of the transfer carrier PACPR6 - HRSYKI 4 (ATCC 67203). 7. Method for preparing recombinant baculoviruses containing DNA fragments that at least encoding a portion of the HBS AG antigen or the pre-S2 protein. 8. A method for preparing plasmid transfer media capable of replication in bacteria and adaptable to integrate with Bacuvovirus containing a DNA fragment that codes for at least part of the HBSAG antigen or the pre-S2 protein. (8 claims, 1 page, 5 images)