TH37593A - Enzyme in starch biosynthesis - Google Patents

Abstract

DC60 (07/07/41) This prototype involves isolated nucleic acid fragments that explicitly code for all or part of the plant glycogenin or water stress proteins. The prototype also involves the structure of a chimeric gene encoding all or part of the plant glycogenin or water stress proteins in the sense and antisense orientation. Here, the expression of the chimeric gene affects the production of levels of plant glycogenin or water stress proteins. This prototype involves isolated nucleic acid fragments that explicitly code for all or part of the plant glycogenin or water stress proteins. The prototype also involves the structure of a chimeric gene encoding all or part of the plant glycogenin or water stress proteins in the sense and antisense orientation. Here, the expression of the chimeric gene... It affects the production of glycogenin levels from plants or proteins, in relation to water stress.

Claims (9)

1. The isolated nucleic acid fragments consist of units selected from the following groups: a) isolated nucleic acid fragments that code for all or part of the predefined amino acid sequence regions in the selected units SEQ ID NO:2, 10, 12, 14, 16, 18, 20, 22, 24, and 26; b) isolated nucleic acid fragments that are clearly similar to the isolated nucleic acid fragments that code for all or part of the predefined amino acid sequence regions in the selected units SEQ ID NO:2, 10, 12, 14, 16, 18, 20, 22, 24, and 26; and c) isolated nucleic acid fragments that are complementary to a) or b). 2. The isolated nucleic acid fragments claiming claim 1 here have nucleotide sequences of the specified fragments located in units selected from a group consisting of SEQ ID NO: 1, 9, 11, 13, 15, 17, 19, 21, 23, and 25. 3. The dimeric gene consists of nucleic acid fragments of trustee 1 that are manipulated to the appropriate regulatory sequence. 4. Transforming host cells using the chimeric gene of claim 3 and... 5. A method for altering the expression levels of plant glycogenin or hydrostimulatory proteins in host cells was investigated using a) transformation of host cells containing the chimeric gene of patent 3'' and b) growth of the transformed host cells in step (a) under conditions optimized for chimeric gene expression. Here, chimeric gene expression resulted in altered levels of plant glycogenin or hydrostimulatory proteins in the transformed host cells. 6. Methods for obtaining nucleic acid fragments encoding the complete amino acid sequence or the explicit amino acid sequence encoding plant glycogenin or hydrostress-related proteins consisting of: a) tracing cDNA or a genome library containing the nucleic acid fragment of claim 1; b) identifying DNA clones hybridized with the nucleic acid fragment of claim 1; and c) sequencing dNDA or genome fragments containing the clones identified in step (c). Here, the sequenced nucleic acid fragments encoding the explicit partial amino acid sequence encoding plant glycogenin or hydrostress-related proteins. 7. The method for obtaining nucleic acid fragments that encode regions of the amino acid sequence encoding plant glycogenin or hydrostress proteins consists of: a) Synthesis of oligonucleotide primers corresponding to the above-mentioned sequence regions in units selected from the group EQ ID NO: 1, 9, 11, 13, 15, 17, 19, 21, 23, and 25; and b) Amplification of the cDNA correspondences present in the cloning vector using the oligonucleotide primers from step (a) and primers representing the sequences of the cloning vector. Here, the amplified nucleic acid fragments encode parts of the amino acid sequence encoding plant glycogenin or hydrostress proteins. 8. The product of the claim method 6. 9. Output of the Claim Method 7 (9 claims, 2 pages, 3 figures)