TH36022B - Methods for the production of base amino acids. - Google Patents

Abstract

DC60 (20/04/53) When amino acid bases are produced by culturing capable microorganisms Base amino acids in liquid culture medium under aeration conditions for the production and accumulation of basal amino acids in the medium, the pH of the medium was controlled to 6.5-9.0 during incubation. cultured and 7.2-9.0 at the end of culture, and at the culture interval where 2 g/L or more of the hydrogen carbonate ion and/or the carbonate ions present in the culture medium were Stabilize it during culturing by controlling the pressure in the fermentation tank to be positive pressure during fermentation or adding carbon dioxide gas. or a gas mixture containing carbon dioxide gas to culture medium, to provide hydrogen carbonate ions and/or carbonate ions will act as The opposite ions of the cations that make up most of the base amino acids. When amino baseic acids are produced by culturing a microorganism capable of producing bases, Aminos in a liquid medium under aerobic conditions to produce and accumulate basic ami acid No bases in the agar medium were controlled to 6.5-9.0 during incubation, and 7.2-9.0 at the end of incubation, and at the incubation period at 2 g/L or greater. of ions The hydrogen carbonate and/or carbonate ions contained in the culture medium were Stabilize it during culturing by controlling the pressure in the fermentation tank to be positive pressure during fermentation or adding carbon dioxide gas. or a gas mixture containing carbon dioxide gas to agar to make hydrogen carbonate ions and/or carbonate ions will act as The opposite ions of the cations that make up most of the base amino acids.

Claims (8)

Disclaimers (all) that will not appear on the advertisement page: 1. A method for the production of basic ammino acid or fermented water or products. Fermentation consisting of amino acid-base amino acids by fermentation that consists of micro-culture processes. Capable of living in liquid culture media under aeration conditions to produce and accumulate base amino acids. In culture medium where: pH of the medium is regulated to 6.5-9.0 during culture, and 7.2-9.0 at the end of Culture and culture interval at 2 g / l or more of hydrogen carbonate ions and / or Carbonate ions present in the culture medium are maintained during culture by pressure control in the tank. Ferment to be at a positive pressure during fermentation or the distribution of carbon dioxide or mixed gas containing carbon dioxide to the feed medium, so that the hydrogen carbonate and / or the ion charge should be performed. Function as the opposite charge of base amino acids. 2. Methods for the production of amino acid-base or weight or composite fermentation products. With base amino acids according to claim 1 where the pressure in the fermenter is 0.03-0.2 MPa. 3. Methods for producing amino acid-base or weight or composite fermentation products. With base amino acids according to claim 1 or 2, where base amino acids consist of one part More than one type of amino acid is selected from L-Lysine, L-Arginine and Ale-Histidine. 4.Methods for the production of fermented water or fermentation products containing base amino acids. By fermentation that consists of the process of culturing microorganisms capable of producing amino acids. Base in liquid culture media under airtight conditions to produce And accumulation of base amino acids in the culture medium by the said fermentation It is composed of hydrogen carbonate ions and / or carbonate ions, which are the opposite of the cation ions that make up the main base amino acids. The normal ratio is 5-80% where the rate is calculated according to the following equation. Normality rate = [normality of hydrogen carbonate ions and / or carbonate ions] / [norms of the constituent cations of amino acids. No base type] x 100 5. Method according to claim 4, whereby amino acids are composed of one or more types of amino acids, which are selected from lysine, ale-arginine and Ele - histidine 6.Methods according to any one of claims 1 through 5 that also comprise e-emission procedures. Carbon dioxide from fermented water or fermentation products 7.Methods according to any one of claims 1 through 6 where the microbes are Corine form Bacteria or Escherichia coli 8. Method for claim 7, where Cory Form Bacteria is Corynebacterium glutamicum