TH35885B - Recombinant preparation (Recombinant) Factor VIII in Protein-Free Culture Diets - Google Patents

Abstract

DC60 (24/03/41) Recombinant (recombinant) Factor VIII that can be produced in relatively large quantities. Continuously extracted from mammalian cells without the presence of any animal-derived proteins such as albumin by cell culture in protein-free cultured medium supplemented with poly. Old copolymers, which are often present in trace metals very small quantities) such as copper, in a very favorable manner, culture food It will cover Known polyols as Pluronic F-68, copper sulphate, ferrous sulphate/EDTA complexes, and tracemetal salts such as Manganese, Molybdenum, Silicon, Lithium, and Chromium Recombinant (recomvinant) Factor VIII that can be produced in relatively large quantities. Continuously extracted from mammalian cells without the presence of any animal-derived proteins such as albumin by cell culture in protein-free cultured medium supplemented with poly. Old copolymers, which are often present in trace metals very small quantities) such as copper, in a very favorable manner, culture food It will cover Known polyols as Pluronic F-68, copper sulphate, ferrous sulphate/EDTA complexes, and tracemetal salts such as manganese, molybdenum, silicon, lithium, and chrome

Claims (1)

Disclaimer (all) which will not appear on the advertisement page: 1. Methods for producing Vlll recombinant factor from mammalian cells. That there is a genetics for this. Which consists of Live cell culture that is a mammalian cell With such milk In protein-free cultured foods derived from plasma 2. The method of claim 1, where the polyole is pluronic F-68 and appears to be used in culture media. The concentrations ranged from 0.1 to 0.3% by weight. 3. Method of claim 1, whereby the above cultured feed included copper sulphates. The quantities ranged from 50 to 800 nanomol 4. Method of claim 1, where in the culture medium consisted of MnSo4 at 3 nanomol 5. Method of claim 1, where That in cultured food contains additional [NH4] 6Mo7O24,4H2O at 3 nanomol 6. Method of claim 1, in which the cultured feed contains additional CrK (SO4) 7,4H2O at 1.5 nanomol 7. Method of claim 1, where in the culture medium contains LiCl at 236nm 8. Method of claim 1, where The living cells that are mammalian cells Selected from a group consisting of baby hamster kidney cells, human embryonic kidney cells, Chinese hamster ovary cells 9. Cell cultures for the production of recombinant factor VIII containing Protein-free base cultures derived from plasma and include pollol and copper ions 1 0. The culture medium of claim No. 9}, which includes, at a minimum, tracemetal. (tace metal, a metal element that appears to be present in very small quantities) one type of choice from A group containing manganese, molybdenum, silicon, chromium and lithium 1. 1.Culture food of claim 10, which contains additional insulin