TH3340B - Polypeptide Processing of Plasmodium Falciparum from Cloned cDNA Clones - Google Patents

Abstract

This invention discusses the fabrication of DNA which consists of polynucleotide sequences. which corresponds to some or all of Plasmodium falciparum messenger RNA (mRNA) or genomic DNA (gDNA). This fabricated ANA consists of polynucleo sequences. A tide that is the code of all or part of one or more P. falciparum proteins, or is a polypeptide, precursor or modification of such protein. This DNA molecule is prepared can decode polypettide These synthetic peptides or polypeptides have antigenic properties. Some or all of the P. falciparum antigens, the constituents of substances that stimulate mammals to produce Immunity to P. falciparum antigen, consisting of polypeptides At least one polypeptide containing P. falciparum antigenicity mixed with a known medicinal vector.

Claims (2)

1. The invention method of recombinant DNA containing a nucleotide body that can be regarded as fully or partially compliant with the RNA of P. falciparum, with the neutron This cleotide is a code for the andigenic polypeptide in the term The asexual divide in the erythrocyte of at least one P. falciparum. 2. The recombinant dNA molecule fabrication method follows. Claim 1, in which the order of the nucleotides is The code of the parts of the polypeptide or all of the polypeptide. At least two species of P. falciparum obtained from one isolate. Variant Or greater than that of P. falciparum 3. The invention of recombinant DNA molecules according to Claim 1 that the said nucleotide sequence is the code of The polypeptide of P. falciparum, which is considered to be consistent with the S antigen of P. falciparum. 4. The recombinant DNA molecule fabrication process follows. Claim 1 that the aforementioned nucleotide sequence consists of: At least the epitope of a partial sequence is shown in Figure 1. 5. The process of recombinant dNA molecule fabrication according to Claim 1 that the aforementioned nucleotide sequence consists of: At least the epitope of partial sequencing is shown in Figures 2a and 2B. 6. The recombinant DNA molecule fabrication process that Contains nucleotide sequences that can be transcribed to at least one polypeptide showing antigenic CD of P. falciparum antigen in the asexual division of the red blood cell. 7. Recombinant DNA molecule invention that Contains a nucleotide sequence. According to claims 1 to 6, link and work with the transcription control sequence. 8. The recombinant DNA molecule invention method follows. Claim 7, where the decoding control sequence, consists of the promoter sequence and the inclusion sequence. And loud nucleotides Addressed on the 3 \ 'side of promoter and sequence of iniculators. 9. The process of fabricating vectors or vectors for recom cloning. Deciphered binanthides for all polypeptides or It is partial to P. falciparum in the asexual division. In red blood cells And one of the nucleotide sequences from Clause 1 to 6 is inserted. In the aforementioned order, welding and Working with the transcription control sequence 1 0. The recombinant DNA molecule fabrication method follows Claim 9, where decryption control sequence, consists of sequence Promoter and sequence of iniculators And that nucleotide is on the 3 \ 'side of the promoter sequence. And sequence of inositol 1. 1. Process for fabrication of recombinantd vectors. NA according to claim 9, characterized by the nucleotide sequence The decoding control sequence was implanted in Bacteriophage 1. 2. The fabrication process for DNA cloning according to claim 11, with the characteristics of such bacteria as bacteria. Rio Phase 2 Amp3 1 3. The process of fabricating household cells with oval molecules. Combined TNA according to Claim 7 or Carrier or Wake Cloning of recombinant DNA as described in Claim 9 1 4. Process for fabricating peptides or synthetic polypeptides. The antigenic activity of P. falciparum antigens in the asexual division in whole or partial erythrocyte phase 1 5. Method for fabrication of synthetic peptides or polypeptides. According to claim 14, in the manner in which it exhibits the total or partial antibiotic of the S antigen of P. falciparum 1 6. The process of fabricating peptides or synthetic polypeptides. According to claim 14, with characteristics considered to be consistent With antigens characterized in Figures 7 through 14 and in Tables 1 to 4, wholly or partially 1 7. The fabrication process of a welded polypeptide consisting of a strand. Deactivate polypeptides showing the activity of P. falciparum antigens in the asexual division in red blood cells. The carbon tip and the additional polypeptide at the tip Welded nitrogen 1 8. Polypeptide welding fabrication process. According to claim 17, where the additional polypeptide is a part of the polypeptide Caused by the code of recombinant DNA carrier 1 9. Production process of welded polypeptide According to claim 17, which consists of a house cell culture procedure under claim 13 and a collection of polypeptides welded from Culture 2 0. Polypeptide fabrication process welded by the Produced according to claim 19 2 1. Polypeptide production process according to claim 14, which consists of cutting process of welded polypeptide according to claim 20 and separating the desired polypeptide from the polypeptide. Lipeb Tide Additional Section 2 2. Polypeptide production process according to the method in claim 21 (22 claims, 3 pages, 14 figures).