TH3085B - Bacillus strain preparation Thru Ring Genesees That have better properties against lapytitters pests And new strains produced from the aforementioned processes - Google Patents

Abstract

It is an invention of Bacillus Thru Ring Genesis GC 91, specimen deposited under Parties NCTC 11821 or its subspecies. Or mutants of such species that have insecticidal properties against lapico terrestrial pests. And related to the production of Bacillus Thru-Ring Genesis That has improved the insecticidal properties as well It was merged into a single species by means of different insecticidal migration of the two initial strains, which were useful as an integral component in the constituents. Kill insects

Claims (1)

1. Bacillus Thru Ring Genesis GC 91, which has deposited a specimen of it under the Parties NCTC 11821 or its subspecies. Or a mutant of such species that has insecticidal properties against the lapidothermic pests. 2. Production process of Bacillus Thru-Ring Genesis. To get cooked, insecticidal properties By merging into the same species with different insecticidal properties of the two initial strains, respectively. Which consists of (a) selecting a mutant from the first initial species with a special character. Is the loss of the smidfish code for the polypeptide, which forms part of the protein seal, and (b) moves one smid. Which contains the code for the synthesis of the kelta endotoxin seal from the asporogenic mutant of the second starting species. The majority of the smid fish, as well as those of the second initial species, enter the production mutants in step (a) in order to produce the desired new species. The first is HD 135, and the mutant produced in step (a) is the 135-S4 (SCTC 11822). It holds 2 or 3 rights where the second starting species is HD 191 and the asphorogenic mutant is 191-A2 (NCTC 11823). 5. Bacillus production process. Strurling Genesis GC 91 (NCTC 11821), which includes the move from Basilus Thru Ring Genesis. Strain 191-A2 (NCTC 11823) enters the basilus thru ring genesis 135-S4 (NCTC 11822), where one smid It has a code for delta-endotoxin synthesis in strains 191-A2 6. Production process, as in claim 5, where 191-A2 and 135-S4 strains are cultured simultaneously in the substrate. Mixed culture, which will cause the moving of the smid that is similar to the articulation (conjugation-like plasmid transfer) and diluted the cultured mixture. And move into solid medium To get a single colony The colonies of GC 91 strains were selected based on the increased size of the parasparagus. The GC 91 strains were then cultured. 7. Insecticide derivatives from Bacillus Thru-Ringgenicil strains GC 91 (NCTC 11821) or from their derivatives. Or the mutant of the aforementioned species Or from the strains produced by the process in claim 2 by the insecticides are spore-crystalline complexes. 8. Basilus Threring G-based pesticides composition. Nisses strain GC 91 (NCTC 11821) or its derivative or mutant. Or pesticides as in claim 7, together with carriers to dilute Surfactant Or additives to stimulate the use (application-promoting adjuvant) 9. Elements as in claim 8, which also contain bioactive compounds selected from fertilizers. Micronutrient donors, other preparations that cause plant growth, herbicides, pesticides, fungicides, bactericides, parasites and shellfish and their mixtures 1 0. Composition as in Holds 8, consisting of Bacillus Thrugenesis GC 91 or its derivatives or mutants. Or the pesticides mentioned in claim 7 from 0.1-99% by weight. Solid additives Or 1-99.9% liquid by weight and surfactant from 0.25% by weight 1 1.How to get rid of lapidotaurus pests Which consists of the use of bacillus Thru Ring Genesis strain GC 91 (NCTC 11821) or its derivatives or mutants Or pesticides as in claim 7 or elements containing the aforementioned species Mutant derivatives Or such substances in quantities that have an effect to kill insects against such pests. Or use in the environment of such pests