TH25833Y - Decolorization process of extract and fractionation of rhodomyrtone from Rhodomyrtus tomentosa (Ait.) Hassk. Leaves - Google Patents

Abstract

OCR 09WP 16/06/2025 The process of reducing the color of the extract and separating the rhodomythone from the leaves of the kratom has the following steps: preparation of the leaves, preliminary removal of chlorophyll color from the leaves, extraction of the important substances in the leaves, absorption of chlorophyll color from the solution, concentration of the important substances from the leaves, separation of the extract, separating the rhodomythone, concentrating the extract, which will result in the extract, separating the rhodomythone from the leaves that is a light brown powder.

Claims (4)

OCR 09WP 16/06/2568 1. The process of decolorizing the extract and separating the rhodomyrtone fraction from the leaves of the Malabar spinach plant involves the following steps: leaf preparation, preliminary chlorophyll removal from the leaves, extraction of active compounds from the leaves, chlorophyll removal from the solution, concentration of active compounds from the leaves, separation of the rhodomyrtone extract, and concentration of the rhodomyrtone extract. The details are as follows: a. Leaf preparation: Select healthy and disease-free leaves, wash them thoroughly with water, and dry them in a hot air oven at 50 to 60 degrees Celsius. Then, grind the dried leaves finely enough for extraction and store the powder in a sealed, light-protective container to prevent degradation of the active compounds. This step yields finely ground leaf powder. b. Preliminary chlorophyll removal from the leaves. The finely ground kratom leaf powder from step A is boiled with water in a ratio of kratom leaf powder to water of 1:3 for 30-60 minutes. The water is filtered and discarded. This step yields kratom leaf powder with some chlorophyll removed. C. Extraction of active compounds from kratom leaves: The kratom leaf powder from step B is air-dried and soaked in 95% ethanol in a ratio of kratom leaf powder to ethanol of 1:2 for 3-5 days. The extraction is repeated at least 3-5 times. All obtained solutions are filtered through a coarse filter. This step yields a solution containing the active compounds from kratom leaves. D. Chlorophyll removal from the solution: Activated charcoal is added to the solution containing active compounds from kratom leaves from step C at 60-70°C and stirred thoroughly. This step yields a solution containing the active compounds from kratom leaves mixed with activated charcoal. E. Concentration of active compounds from kratom leaves: The solution containing the active compounds from kratom leaves is concentrated by... Step 4: Separation of the rhodomyrtone extract. The crude extract from step e is dissolved in a fine-penetrating solvent until all the charcoal powder is removed. The resulting solution is then evaporated under vacuum to ensure no solvent remains. This step yields a crude extract from the dark brown leaves of the rhodomyrtone plant. Step 2: Separation of the rhodomyrtone extract. The crude extract from the brown leaves (from step e) is dissolved in a low-polar solvent. Coarse silica gel is added to the crude extract solution and stirred until it dries into a loose powder. This powder is then slowly transferred into a column (a chromatography column with a diameter of 8-10 cm, using hexane as a wetting solvent to maintain a smooth surface). A hexane-ethyl acetate mixture (hexane to ethyl acetate ratio of 100:0 to 94:6) is used to elute the semi-purified extract from the column. This step yields a rhodomyrtone fractional extract. To concentrate the rhodomyrtone fractional extract, evaporate the solvent from the extractive solution from step F under reduced pressure for 1-2 hours, repeating 4-5 times. 2. Method for decolorizing the extract and separating the rhodomyrtone fraction from the leaves of *Kratuek* (a type of vine), as per claim 1. In step 6, the rhodomyrtone fraction is separated by dissolving the crude brown extract from the leaves (from step 7) in a low-polar solvent. The low-polar solvent can be selected from hexane, dichloromethane, chloroform, or ether. 3. A method for decolorizing the extract and separating the rhodomyrtone compounds from the leaves of the Indian cork tree, as per claim 2, where the low-polar solvent is dichloromethane. 4. A method for decolorizing the extract and separating the rhodomyrtone compound from the Botanical tree, according to any one of claims 1-3, which will yield a rhodomyrtone extract that is a light brown powder.