TH19364C3 - Saccharomycescerevisiae hybrid yeast cells for the production of terpenes. - Google Patents

Abstract

Summary of the invention which will appear on the advert page ReadFile:------14/08/2020------(OCR)This invention aims to genetically improve the yeast Saccharomycescerevisiae to obtain cells. Yeast hybrid S.cerevisiae for the production of terpene essential substances such as beta-eudesmol and 10-epi-gamma-udesmol (10-epi-gamma- eudesmol, etc., which is a one-step fermentation in which the hybrid yeast is genetically modified to express terpenesynthase enzymes such as beta-udesmolsinte. The beta-eudesmolsynthase derived from the bantam plant and the enhanced ability of such yeast hybrids to produce a large amount of farnesylpyrophosphate intermediates reduces costs and shortens the lead time. In the production and extraction of terpene essential oils--------------Page 1 of 1 Summary of Invention This invention aims to genetically improve the yeast Saccharomycescerevisioe for A hybrid S.cerevisioe yeast cell was obtained for the production of terpenes such as beta-eudesmol and 10-epi-gamma-udesmol (10). This is a one-step fermentation in which the hybrid yeast is genetically modified to express the expression of terpenesynthase enzymes such as beta-eudesmol. The beta-eudesmolsynthase derived from the quail plant and the increased ability of such yeast hybrids to produce high levels of farnesylpyrophosphate intermediates was therefore reduced. Cost and time reduction in the production and extraction of terpene essential oils

Claims (9)

Claims (total) which will not appear on the classifieds page:------14/08/2020------(OCR) 1.Saccharomycescerevisiae hybrid yeast cell BMGC305 for the production of bulk essential oils. Terpenes arranged for the expression of enzymatic transcription genes in the terpenesintase group by the transcription genes in those groups have a nucleotide sequence according to SEQIDNO. 1 which is the nucleotide sequence obtained from the kratom 2. Claim 1 hybrid yeast cell, where the enzyme transcription of the terpenesinase family is the ZzZSS2 gene. 3. A hybrid yeast cell according to claim 1 or 2, where the aforementioned terpenesinase enzyme is beta-udesmalsintase. 4.Hybrid yeast cells pursuant to one of Claims 1-3, where the terpene synthase enzyme transcription gene is regulated by the nucleotide sequence. Promoter region otides can be selected from either a promoter with a constant expression level control or a promoter that requires an induction of expression. 5.Hybrid yeast cells according to claim 4, where appropriate promoters are promoters that require induction of expression. 6.Hybrid yeast cells according to claim 5, where the promoter relied on induction for such expression is selected from either the promoter of the GAL1 gene or the promoter of the GAL10 gene. 7.Hybrid yeast cells according to claim 6, where the promoter relied on induction for optimal expression is the promoter of the GAL10 gene. 8. Hybrid yeast cells under Claim 1, where S.cerevisiae yeast host cells are selected from natural (wild-type), strain S288C, strain BY4742, strain CEN.PK, strain W303, strain. A364A strain, FL100 strain, EPY300 strain or one of the modified strains producing high amounts of farnecil pyrophosphate. 9. Hybrid Yeast Cells under Claim 1 or 8, where the most suitable host S.cerevisiae yeast cell is strain EPY300------------Revised Nov 2,18 No Claim. ++++++++++++++++++++++++++ Page 1 of the number 1 claim page 1.Hybrid yeast cells SaccharomycescerevisiaeBMGC305 for the production of terpene enzymes are arranged for expression of terpenesinase gene transcription by enzyme transcription gene. The nucleotite sequences were sequenced according to SEQIDNO.1, which is a nucleotide sequence obtained from kratom. 2. Claim 1 hybrid yeast cell, where the enzyme transcription of the terpenesinase family is the ZzZSS2 gene. 3. A hybrid yeast cell according to claim 1 or 2, where the aforementioned terpenesinase enzyme is beta-udesmalsintase. 4.Hybrid yeast cells pursuant to one of Claims 1-3, where the terpene synthase enzymatic transcription gene is regulated in the expression of that gene by a bucle sequence. The promoter region otite can be selected from either a promoter with a fixed expression level control or a promoter that requires an induction of expression. 5.Hybrid yeast cells according to claim 4, where appropriate promoters are promoters that require induction of expression. 6.Hybrid yeast cells according to claim 5, where the promoter relied on induction for such expression is selected from either the promoter of the GAL1 gene or the promoter of the GAL10 gene. 7.Hybrid yeast cells according to claim 6, where the promoter relied on induction for optimal expression is the promoter of the GAL10 gene. 8. Hybrid yeast cells under Claim 1 where S.cerevisiae yeast host cells are selected from natural (wild-type), strain S288C, strain BY4742, strain CEN.PK, strain W303, strain. A364A strain, FL100 strain, EPY300 strain or one of the modified strains producing high amounts of farnecil pyrophosphate. 9. Hybrid yeast cells according to claim 1 or 8, where the most suitable S.cerevisiae yeast host cell is strain EPY300.