TH1801003911A - Carrimycin biosynthetic gene family - Google Patents

Claims (10)

1. Revised 12/09/2018 Claim 1. The carrimycin biosynthetic gene family, which comprises 44 genes, includes: 1) five polyketide synthase genes; That includes orf10-14; 2) Nine genes involved in expansion units in the synthesis and modification of polyketones including orf1,orf4-6,15 and 36-39; 3) Sixteen genes involved in glycosyl synthesis including orf9,16-22,24,26,28,29, 33-35 and 41; 4) Six genes involved in glycosyltransduction including orf7,8,30-32 and 40; 5) Two genes involved in resistance include orf3 and 25; 6) Four genes involved in biosynthesis regulation including orf2,23,27 and 42; and 7) Two genes including the splicing marker gene exogenus orf43 and the mycarose 4"-O-hydroxylisovaleryltransferase orf44-linked gene. Continue with orf43 ---------- Page 1 of 2 Pages Claim 1. Gene family for carrimycin biosynthesis, containing 44 genes containing: 1 ) five polyketide system genes, including orf10-14; 2) nine genes involved in modification and elongation units for synthesis Polyketones, including orf1,orf4-6,15 and 36-39; 3) Sixteen genes involved in glycosyl synthesis, including orf9,16-22,24,26,28, 29,33-35 and 41; 4) Six genes involved in glycosyltransfer, including orf7,8,30-32 and 40; 5) two tolerance-related genes, including orf3 and 25; 6) four genes involved in the regulation of biosynthesis, including orf2,23,27 and 42; and 7) two genes, including the engineered marker genes of the exogenous genes orf43 and Mycaros 4"-O-hydroxyylisovaleryltransferase orf44 connected to orf43 2. A gene family for biosynthesis according to claim 1, characterized by Five polyketide synthase genes code for polyketide biosynthesis enzymes, enzymes. The biosynthetic polyketide catalyzes the synthesis of the 16-membered lactone ring. of carrimycin, and The amino acid sequence corresponding to the nucleotide sequence or the equivalent sequence of the 5 polyketide synthase. The orf10-14 gene includes IA-W10,IA-W11,IA-W12,IA-W13 and IA-W14. 3. Gene groups for biosynthesis according to claim 1 or claim 2, containing The uniqueness that the five polyketide synthase contains an amino acid sequence corresponding to The nucleotide sequences or equivalent sequences of the structural domains of ketosyntheses (KS), acyltransferase (AT), keto reductase (KR), dehydrated (DH), enoyl reductase (ER), acil Carrier protein (acylcarrierprotein) (ACP) and thioesterase 4. The gene family for biosynthesis according to claim 3, characterized by Five polyketide synthases are composed of structural modules or domains. 2nd page of 2 pages 5. A gene family for biosynthesis according to claim 1, characterized by that sequence an amino acid that corresponds to the nucleotide sequence or sequence of the gene associated with Modifications and elongation units for the synthesis of polyketones including orf1,orf4-6,15. and 36-39 include IA-W1,IA-W4,IA-W5,IA-W6,IA-W15,IA-W36,IA-W37, IA-W38 and IA-W39. 6. A gene family for biosynthesis according to claim 1, characterized by that sequence The amino acids corresponding to the sequences of genes involved in glycosyl biosynthesis include: orf9,16-22,24,26,28,29,33-35 and 41 includes IA-W9,IA-W16,IA-W17,IA- W18,IA-W19,IA-W20,IA-W21, IA-W22,IA-W24,IA-W26,IA-W28,IA-W29,IA-W33, IA-W34,IA-W35 and IA-W41 7. A gene family for biosynthesis according to claim 1, characterized by that sequence An amino acid corresponding to a nucleotide sequence or to the sequence of a glycosyl-related gene. Transfers that include orf7,8,30-32 and 40 include the IA-W7,IA-W8,IA-W30, IA-W31,IA-W32 and IA-W40. 8. A gene family for biosynthesis according to claim 1, characterized by that sequence amino acids corresponding to nucleotide sequences or sequences of tolerance-related genes That includes orf3 and 25 includes the IA-W3 and IA-W25. 9. A gene family for biosynthesis according to claim 1, characterized by that sequence amino acids corresponding to nucleotide sequences or sequences of genes involved in Biosynthetic regulators including orf2,23,27 and 42 include IA-W2, IA-W23,IA-W27 and IA-W42. 10. A gene family for biosynthesis according to claim 1, characterized by that sequence amino acids corresponding to nucleotide sequences or to sequences of marker-related genes The engineered genes of the exogenous genes orf43 and orf44 connected to the orf43 assembly. Included IA-W43 and IA-W44;