TH17657C3 - Reagents for the preservation of conjugates and conjugated plates, including molecules used in signaling or amplifying and the treatment methods for preparing conjugates, including plates. - Google Patents

Abstract

Summary of the invention which will appear on the advertisement page ReadFile:------21/02/2020------(OCR)Page 1 of the 1 page summary of the invention of a solution for preserving the quality of Kon. Jugates and conjugated plates, including molecules used in signaling or amplification and procedures for the preparation of reagents for the preparation of conjugates and conjugated plates by reagents. For such quality treatment it consists of buffer solutions, non-specific binding agents and dimerized or stringed carbohydrates, and may contain additional antimicrobial agents by the method of treatment. The preparation of conjugates for use in the test kit consists of mixing the conjugate into the solution and loading the conjugate mixture into the conjugate plates. The test kit gate includes the method of using such reagents for the preparation of conjugated plates or signal amplifying molecules for use with the test kit. or molecules used in the signaling or amplification are pretreated with such reagents by soaking and/or spraying, drying and preserving of such packs. It was developed so that it can be applied to maintain the quality of such conjugates and plates so that they have the ability to bind to the target molecule and can provide or amplify the signal to enhance it. The efficacy of conjugate-conjugated assays involving chromatography and targetingligands in a highly functional and functional environment may also help. It also increases the efficiency of assays where assays are based on the principle of detection of target molecules by horizontal flow chromatography or side flow chromatography or immersion type or vertical type by the said process. It can be applied in the detection of various target molecules such as pathogens, chemical molecules, drugs, various biomarkers and specific target substances to be detected, etc. 1 of 1 page summary of fabrication of conjugated reagents, including plates containing conjugates and/or molecules used to provide or amplify signals and procedures for use in preparation of reagents. Conjugates, including conjugated plates, were developed so that they could be applied to maintain the quality of Such anjugates and plates retain the ability to bind to the target molecule and can provide or amplify signals to enhance the performance of conjugated assays relevant to chromatography. Matography and targetingligands can be highly functional and maintainable, and may also improve the efficiency of assays where assays are based on the principle of detection of target molecules. Horizontal flow chromatography or lateral flow or immersion or vertical type chromatography by such methods can be applied in the detection of various target molecules such as pathogens, substances chemistry, drug molecules, various biomarkers, and specific target substances to be detected, etc.

Claims (8)

Claims (all), which will not appear on the classifieds page:------21/02/2020------(OCR)Page 1 of 2 Claims Pages. 1. Reagents for the preservation of conjugated substances and conjugated plates, including molecules used to administer or amplify the reagents consist of a. at least one selectable buffer solution from Tris-Hi. Hydrochloride buffer, borate buffer, borax buffer, phosphate buffer, or carbonate buffer where the buffer solution is 0.1-100 mmolar b. Choose at least one from bowine albumin, egg albumin, human albumin, casein protein, skim milk, or polymer. Polyvinylpyrrolidone, where the non-specific binding agent accounts for 0.1-40% by weight of the total mixture and dimerized or selective carbohydrates. At least one from maltodextrin, hydroxylpropyl beta-cyclodextrin, beta-cyclodextrin, cyclodextrin, manni. Tol, trehalose, or raffinose, where dimerized or chain carbohydrates account for 0.1-40% by weight of the total mixture to which the buffer solution is added to the adjusting solution. Let all ingredients be 100% by weight. 2. Reagents for the treatment of conjugate and conjugated plates, including molecules used for signaling or amplification according to Claim 1, are supplemented with selected antimicrobial agents. At least one type of sodium azide or thymerrosol was obtained by preventing microbial growth of 0.01-0.1% by weight of the total mixture. 3. Reagents for the treatment of conjugated substances and conjugated plates, including molecules used to induce or amplify claims 1 or 2 where the reagent is applied to the test kit. Choose one or more from the target molecule detection test kit or the signaling test kit. 4. Reagents for preserving conjugates and conjugated plates, including molecules used in Claim 3 signaling or amplification where at least one target molecule is selected from the pathogen. Disease chemical molecules, biomarkers, hormones and at least one selectable specific target from biomolecules. 5. Reagents for the treatment of conjugates and conjugated plates, including molecules used in Claim 3 or 4, where test kits for the detection of target molecules rely on the technique. Horizontal flow type chromatography, lateral flow type, immersion type and vertical type, either page 2 of 2 pages. 6. Reagents for the treatment of conjugated substances and conjugated plates, including molecules used for signaling or amplifying according to Claim 3, where the test kit for which the signal is displayed contains a molecule. that is specific to one or more selectable target molecules from antibody molecules, fragments or synthetic derivatives of antibody fragments of nucleic acid RNA strands. Tamer peptides, PNA avidin and biotin, and b. at least one selectable amplifying/amplifying molecule from gold nanoparticles, metal-based nanoparticles and polysaccharides. Inorganic pigments, coloring enzymes, fluorescence signaling agents, Raman signaling agents, infrared signaling agents, pigments and polymers. Signaling where molecules specific to the target molecule are labeled with the signaling/amplifying molecule. 7. Methods of use of reagents for preserving the quality of conjugated substances and conjugated plates, including molecules used for signaling or amplifying under Claims 1-6 for the preparation of substances. The conjugate for use with the test kit consists of the following steps: a. Mixing the prepared conjugate with an opaque value of 0.1-30 to dissolve it in the solution to obtain the conjugate mixture; and b. The conjugate mixture was loaded into the conjugated plate of the test kit with a volume of 0.1-10 µL per test kit. 8. Methods of use of reagents for preserving the quality of conjugated substances and conjugated plates, including molecules used to provide or amplify signals under Claims 1-6 for plate preparation. Conjugate/molecules used for amplification/amplification for use in the test kit consists of the following steps: a. The pretreatment of the conjugate/molecules used for amplifying or pretreatment with the reagent For 1-30 minutes by soaking and/or spraying b. Drying such packing sheets at a temperature of 4-37 degrees Celsius, 10-60% relative humidity for 1-12 hours and c. keeping the sheets. The package was dried at 4-25°C with a relative humidity of 10-60% and kept away from light---------------Page 1 of Page 2 Claims. 1. Reagents for the preservation of conjugated substances, including conjugated plates and/or molecules used to induce or amplify the reagents consisting of g. at least one selectable buffer solution from a trise. -Hydrochloride buffers, borate buffers, borax buffers, phosphate buffers, or carbonate buffers where the buffer solution has a concentration of 0.1-100 mmolar b. Not only can choose at least one from Bowine albumin, egg albumin, human albumin, casein protein, skim milk, or enough. A polyvinylpyrrolidone polymer, where non-specific binding agents account for 0.1-40% by weight of the total mixture and dimerized or stranded carbohydrates. Choose one or more from maltodextrin,hydroxypropyl beta-cyclodextrin,beta-cyclodextrin,cyclodextrin, Mannitol, trehalose, or raffinose, where dimerized or chain carbohydrates account for 0.1-40% by weight of the total mixture to which the buffer solution is added to the solution. to adjust all the ingredients to be 100% by weight 2. The reagent according to claim 1 additionally contains at least one selectable antimicrobial agent from sodium azide or thymerrosol as a percentage of antimicrobial agent. 0.01-0.1 by weight of all ingredients 3. Reagents according to claim 1 or 2 where the reagent is applied to at least one selectable test kit from a target molecule test kit or a signaled test kit. 4. Claims 3 reagents where at least one selectable target molecule from a pathogen, chemical drug molecule, hormonal biomarker and at least one selectable specific target from biomolecule. 5. Reagents according to claim 3 or 4 where assays for the detection of target molecules are based on either horizontal flow chromatography, lateral flow, immersion and vertical type chromatography. one page 2 of the number 2 pages 6. Reagents according to claim 3, where the assays for which the signal is displayed consist of a molecule that is specific to one or more selectable target molecules from the antibody molecule, fragment or synthetic derivative. of antibodies, DNA fragments, RNA strands, nucleic acid aptamers, peptides, PNA avidin, and biotin, and b. At least one selectable amplification/amplifier from gold nanoparticles, metallic and inorganic particles, pigment enzymes, fluorescence signaling agents, Raman signaling agents. Colored and signaling polymer infrared signaling, where molecules specific to the target molecule are labeled with signal amplifying/amplifying molecules. 7. Any method of using the reagent according to Claims 1-6 for the preparation of conjugates for use with the procedural test kit consists of a. Mixing of the prepared conjugate to an opaque value of 0.1 -30 to be dissolved in the solution to obtain a conjugate mixture and b. The conjugated particle mixture is loaded into the conjugated plate of the test kit with a volume of 0.1-10 µL/1. test kit 8. Any method of reagent use according to Claims 1-6 for the preparation of conjugate/molecule plates used to provide or amplify signals for use with the test kit consists of: a. The conjugate/molecules used for signal amplification were pretreated with the solution for 1-30 minutes by soaking and/or spraying. 37°C, 10-60% relative humidity for at least 1-12 hours, and c. The storage of such packing sheets that have been dried at 4-25°C with a relative humidity of 10-60% and stored for wrapping. from the light