TH175833B - The preparation of trombin from plasma that separates cryoprocipetate using an activating agent. Factors ten from the poisonous snakes. - Google Patents

The preparation of trombin from plasma that separates cryoprocipetate using an activating agent. Factors ten from the poisonous snakes.

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Publication number
TH175833B
TH175833B TH1701002507A TH1701002507A TH175833B TH 175833 B TH175833 B TH 175833B TH 1701002507 A TH1701002507 A TH 1701002507A TH 1701002507 A TH1701002507 A TH 1701002507A TH 175833 B TH175833 B TH 175833B
Authority
TH
Thailand
Prior art keywords
plasma
preparation
cryoprocipetate
trombin
separates
Prior art date
Application number
TH1701002507A
Other languages
Thai (th)
Other versions
TH175833A (en
Original Assignee
จุฬาลงกรณ์มหาวิทยาลัย
Filing date
Publication date
Application filed by จุฬาลงกรณ์มหาวิทยาลัย filed Critical จุฬาลงกรณ์มหาวิทยาลัย
Publication of TH175833A publication Critical patent/TH175833A/en
Publication of TH175833B publication Critical patent/TH175833B/en

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Claims (1)

1. กระบวนการเตรียมทรอมบินจากพลาสมาที่แยกไครโอปริซิปิเตทโดยใช้สารปลุกฤทธิ์แฟคเตอร์ สิบจากพิษงูแมวเซา ซึ่งมีขั้นตอนการเตรียมดังนี้ ก. เจือจางพลาสมาที่แยกไครโอปริซิปิเตทด้วยน้ำกลั่นจนได้ปริมาตรเพิ่มขึ้นเป็น 8-12 เท่าของปริมาตรพลาสมาเริ่มต้น ข. ปรับพีเอสของสารละลายในข้อ ก. ด้วยกรดอะซิติก 1-3% โดยปริมาตร จนกว่าจะได้ พีเอชในช่วง 4.5-6.0 ค. นำสารละลายในข้อ ข. ไปปั่นในเครื่องหมุนเหวี่ยง ที่ความเร็ว 2000-4000 รอบต่อ นาท1. The preparation of trombin from plasma that separates cryoprocipetate by activating factor. Ten from the venom of the serpent snake The preparation steps are as follows: a. Dilute the cryoprecipitate plasma with distilled water until the volume is increased to 8-12 times the initial plasma volume. B. Adjust the PS of the solution in a With 1-3% acetic acid by volume until a pH in the range of 4.5-6.0 c. The solution in item B is centrifuged in a centrifuge at 2000-4000 revolutions per minute.
TH1701002507A 2017-05-04 The preparation of trombin from plasma that separates cryoprocipetate using an activating agent. Factors ten from the poisonous snakes. TH175833B (en)

Publications (2)

Publication Number Publication Date
TH175833A TH175833A (en) 2018-05-17
TH175833B true TH175833B (en) 2018-05-17

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