TH16951B - Primers and DNA probes for the detection of Campylobacter spp. With loop mediated isothermal reactions. Application (Loop-mediated isothermal amplification (LAMP)), combined with the use of a gold nanoprobe DNA biosensor labeled probe. - Google Patents

Primers and DNA probes for the detection of Campylobacter spp. With loop mediated isothermal reactions. Application (Loop-mediated isothermal amplification (LAMP)), combined with the use of a gold nanoprobe DNA biosensor labeled probe.

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Publication number
TH16951B
TH16951B TH1803000709U TH1803000709U TH16951B TH 16951 B TH16951 B TH 16951B TH 1803000709 U TH1803000709 U TH 1803000709U TH 1803000709 U TH1803000709 U TH 1803000709U TH 16951 B TH16951 B TH 16951B
Authority
TH
Thailand
Prior art keywords
loop
mediated isothermal
dna
detection
lamp
Prior art date
Application number
TH1803000709U
Other languages
Thai (th)
Inventor
จันทร์ศิริ รศ.ดร.โกสุม
อารีกิจ ดร.สุพัตรา
ภาคพิธเจริญ นางอาชว์ดาม์
ขาวสัก นายไพศาล
สันติวัฒนกุล รศ.ดร.สมชาย
ทองเผือก นางสาวเดือนเต็ม
ศรียาภัย ผศ.ดร.ทายาท
ศรียาภัย ผศ.ดร.พิชาภัค
หวังรุ่งทรัพย์ ดร.ปิยะดา
Original Assignee
นางสาวนิยดา รุ่งเรืองผล
Filing date
Publication date
Application filed by นางสาวนิยดา รุ่งเรืองผล filed Critical นางสาวนิยดา รุ่งเรืองผล
Publication of TH16951B publication Critical patent/TH16951B/en

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Abstract

หน้า 1 ของจำนวน 1 หน้า บทสรุปการประดิษฐ์ ชุดไพรเมอร์ (primer) และตัวตรวจจับ (DNA probe) สำหรับตรวจเชื้อแคมไพโลแบคเตอร์ สปีชีส์(Campylobacter spp.) ด้วยปฏิกิริยาปฏิกิริยาลูพเมดดิเอทเตด ไอโซเทอร์มอล แอมพลิฟีเคชั่น (Loop- mediated isothermal amplification (LAMP)) ผนวกกับการใช้ตัวตรวจสอบที่ติดฉลากตัวยอนุภาคทองคำ (Goldnanoprobe DNA biosensor) มีบทสรุปดังนี้ 1. เป็นชุดตรวจอย่างง่าย สะดวก และรวดเร็ว ทราบผลในเวลา 1 ชั่วโมง 30 นาที สามารถ นำไปใช้ตรวจภาคสนาม (point-of-care) ได้ 2. มีความจำเพาะ (specificity) สูง 3. มีความแม่นยำสูงสามารถตรวจพบได้ในระดับปริมาณต่ำสุด (limit of detection) ที่ปริมาณ ดีเอ็นเอระดับประมาณ นาโนกรัม (nanogram, 10-9) ถึง เฟมโตรัม (femtogram, 10-15) 4. ใช้สำหรับตรวจเชื้อเชื้อแคมไพโลแบคเตอร์ สปีชีส์ (Campylobacter spp.) Page 1 of 1 page Summary of the invention of a primer and DNA probe for the detection of campylobacteria. Species (Campylobacter spp.) With loop-mediated isothermal amplification (LAMP) reactions were added to the assay. Examination labeled with gold particles (Goldnanoprobe DNA biosensor) is summarized as follows: 1. It is a simple, convenient and fast test kit. Results in 1 hour 30 minutes can be used for field examination. (point-of-care) 2. High specificity 3. High accuracy Can be detected at the lowest level (limit of detection) at approximately nanogram DNA content (nanogram, 10-9 ) To femtogram (femtogram, 10-15) 4. Used for testing of Campylobacter species (Campylobacter spp.)

Claims (1)

ข้อถือสิทธฺ์ (ทั้งหมด) ซึ่งจะไม่ปรากฏบนหน้าประกาศโฆษณา :Disclaimers (all) that will not appear on the advertisement page:
TH1803000709U 2018-03-26 Primers and DNA probes for the detection of Campylobacter spp. With loop mediated isothermal reactions. Application (Loop-mediated isothermal amplification (LAMP)), combined with the use of a gold nanoprobe DNA biosensor labeled probe. TH16951B (en)

Publications (1)

Publication Number Publication Date
TH16951B true TH16951B (en) 2020-11-24

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