TH16951B - Primers and DNA probes for the detection of Campylobacter spp. With loop mediated isothermal reactions. Application (Loop-mediated isothermal amplification (LAMP)), combined with the use of a gold nanoprobe DNA biosensor labeled probe. - Google Patents

Primers and DNA probes for the detection of Campylobacter spp. With loop mediated isothermal reactions. Application (Loop-mediated isothermal amplification (LAMP)), combined with the use of a gold nanoprobe DNA biosensor labeled probe.

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Publication number
TH16951B
TH16951B TH1803000709U TH1803000709U TH16951B TH 16951 B TH16951 B TH 16951B TH 1803000709 U TH1803000709 U TH 1803000709U TH 1803000709 U TH1803000709 U TH 1803000709U TH 16951 B TH16951 B TH 16951B
Authority
TH
Thailand
Prior art keywords
loop
mediated isothermal
dna
lamp
detection
Prior art date
Application number
TH1803000709U
Other languages
Thai (th)
Inventor
หวังรุ่งทรัพย์ ดรปิยะดา
อารีกิจ ดรสุพัตรา
ทองเผือก นางสาวเดือนเต็ม
ภาคพิธเจริญ นางอาชว์ดาม์
ขาวสัก นายไพศาล
ศรียาภัย ผศดรทายาท
ศรียาภัย ผศดรพิชาภัค
จันทร์ศิริ รศดรโกสุม
สันติวัฒนกุล รศดรสมชาย
Original Assignee
นางสาวนิยดา รุ่งเรืองผล
Filing date
Publication date
Application filed by นางสาวนิยดา รุ่งเรืองผล filed Critical นางสาวนิยดา รุ่งเรืองผล
Publication of TH16951B publication Critical patent/TH16951B/en

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Abstract

หน้า 1 ของจำนวน 1 หน้า บทสรุปการประดิษฐ์ ชุดไพรเมอร์ (primer) และตัวตรวจจับ (DNA probe) สำหรับตรวจเชื้อแคมไพโลแบคเตอร์ สปีชีส์(Campylobacter spp.) ด้วยปฏิกิริยาปฏิกิริยาลูพเมดดิเอทเตด ไอโซเทอร์มอล แอมพลิฟีเคชั่น (Loop- mediated isothermal amplification (LAMP)) ผนวกกับการใช้ตัวตรวจสอบที่ติดฉลากตัวยอนุภาคทองคำ (Goldnanoprobe DNA biosensor) มีบทสรุปดังนี้ 1. เป็นชุดตรวจอย่างง่าย สะดวก และรวดเร็ว ทราบผลในเวลา 1 ชั่วโมง 30 นาที สามารถ นำไปใช้ตรวจภาคสนาม (point-of-care) ได้ 2. มีความจำเพาะ (specificity) สูง 3. มีความแม่นยำสูงสามารถตรวจพบได้ในระดับปริมาณต่ำสุด (limit of detection) ที่ปริมาณ ดีเอ็นเอระดับประมาณ นาโนกรัม (nanogram, 10-9) ถึง เฟมโตรัม (femtogram, 10-15) 4. ใช้สำหรับตรวจเชื้อเชื้อแคมไพโลแบคเตอร์ สปีชีส์ (Campylobacter spp.)Page 1 of 1. Summary of the Invention: A primer and DNA probe kit for detecting Campylobacter spp. using the loop-mediated isothermal amplification (LAMP) reaction, combined with the use of a gold nanoprobe DNA biosensor. The summary is as follows: 1. It is a simple, convenient, and rapid test kit, providing results in 1 hour and 30 minutes. It can be used for field testing. (Point-of-care) Advantages: 2. High specificity. 3. High accuracy, capable of detecting DNA at a minimum level (limit of detection) of approximately nanograms (10⁻⁹) to femtoriums (10⁻¹⁵). 4. Used for detecting Campylobacter species (Campylobacter spp.).

Claims (1)

1. ข้อถือสิทธฺ์ (ทั้งหมด) ซึ่งจะไม่ปรากฏบนหน้าประกาศโฆษณา :1. All claims, which will not appear on the advertisement page:
TH1803000709U 2018-03-26 Primers and DNA probes for the detection of Campylobacter spp. With loop mediated isothermal reactions. Application (Loop-mediated isothermal amplification (LAMP)), combined with the use of a gold nanoprobe DNA biosensor labeled probe. TH16951B (en)

Publications (1)

Publication Number Publication Date
TH16951B true TH16951B (en) 2020-11-24

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