TH16784B - Method for preparing pineapple peels and cores for the production of polyhydroxybutyrate. - Google Patents

Abstract

------ 20/08/2019 ------ (OCR) page 1 of number 1 page invention summary. The method for preparing the coarse juice from the peel and core of pineapple for the production of polyhydroxybutyrate is based on this invention. It consists of the following steps: 1. Peel and cores fresh pineapples, squeezed by a juicer to separate the water and residue until the coarse juice. 2. 0 Coarse juice from item 1. To adjust the acid-base focus in The suitable range of 6.5-7.5 is 7.0 and should be filtered through the No. 1 Waterman filter and should be sterilized at standard temperature conditions. 121 C Under 15 psi pressure for 15 minutes, then microorganism was cultured [using the microorganism Cupriavidus necotor strain A-04 at 30-37 ° C, shaking rate of 100-300 cycles. Per minute for microbial growth and production of polyhydroxybutyrate. The invention is a method of preparing the coarse juice of agro-industrial waste from canned pineapple to produce polyhydroxyl canaate, polyhydroxybutyyrate. (polyhydroxybutyrate, PHB) by using the peel and core of fresh pineapple to focus on raw materials by preparing to focus on coarse juice with single molecule sugar for fermentation using Cupriavidus necotor strain A-04 with the ability to produce polyhydroxybenzoate. Drroxyl canaates are derived from many types of sugar and use different sources of nitrogen. It can also produce polyhydroxyethyl canaate type. Homopolymer and heteropolymer can be used as copolymer and teropolymer, with the production of polyhydroxyethylcylca. Up to 78 percent Noet by mass at 60 hours of production ------------ page 1 of the number 1 page. The process of preparing the coarse juice from pineapple peel and core for the production of polyhydroxybutyrate. According to this invention This is a method for preparing the coarse juice of agro-industrial waste from canned pineapple. To produce polyhydroxyethyl canoate, polyhydroxybutyrate (polyhydroxybutyrate, PHB) fresh pineapple peel and cores were prepared as raw materials with a single sugar molecule for fermentation. By using Cupriovidus necotor strain A-04 capable of producing polyhydroxyethylcalcanoids. E is derived from many types of sugar and uses different nitrogen sources. It can also produce polyhydroxyapatite. Alkanoate type Homopolymer and heteropolymer can be copolymer and thermopolymer. It produces up to 78 percent of polyhydroxyethyl canaate mass at 60 hours of production.

Claims (2)

Disclaimer (all) which will not appear on the advertisement page. : ------ 20/08/2019 ------ (OCR) Page 1 of Number 1 Page. 1. Method for preparing fresh pineapple juice from rind or core for polyhydroxybutyrate production, which consists of the following steps (1.1) Fresh pineapple peel and cores are squeezed by a separate juicer. Water and residue until coarse juice (1.2) Take the coarse juice from item (1.1) to adjust the pH value in the range 6.5-7.5 and filter through the No. 1 Waterman filter paper and then coarse juice. It was then sterilized at standard temperature 121 ° C under pressure of 15 psi for 15 minutes, then the cupriavidus necator strain A-04 was cultured at 30-37 ° C. 100-300 rpm For the production of polyhydroxybutyrate 2. Method of preparation of fresh pineapple juice extracted from peel or core for the production of polyhydroxybutyrate according to claim 1, which also includes additional steps. Coarse-squeezed water from item (1.1) is added 3-5 g / l of disodium hydrogen phosphate (Na2HP04), 1-2 g / l potassium dihydrogen phosphate (KH2PO4), magnesium sulfate, heptahydrate (MS04-7H20). ) 0.1-1.0 g / l Ferric ammonium citrate (Fe (lll) (NH4) citrate) 0.01-0.1 g / l Calcium chloride dihydrate (CaCl2-2H20) 0.01-0.10 g / l And a 1 ml low trace element solution containing boric acid (H3B04) 0.1-0.5 g / l, cobal chloride hexahydrate. (CoCl2 \ '6H20) 0.1-0.5 g / l zinc sulfate heptahydrate (ZnS04-7H20) 0.01-0.03 g / l Manganese chloride tetrahydrate (MnCl2-4H20) 0.01-0.05 g / l Ammonium heptamolybdetetrahydrate ((NH4) 6Mo7024-4H20) 0.01-0.05 g / l, nickel chloride hexahydrate (NiCl2-6H20) 0.01-0.05 g / l and copper sulfate pentahydrate (CuS04-5H20 ) 0.01-0.05 g / l, adjust the carbon / nitrogen ratio in the range 20-200, adjust the pH in the range 6.0-8.0, then filter through the No. 1 Waterman filter paper and then autoclave at standard conditions. The temperature was 121 degrees Celsius under the pressure of 15 psi for 15 minutes. 30-37 degrees Celsius Shaker rate of 100-300 rpm for the production of polyhydroxybutyrate ------------ page 1 of amount 1 page. 1. The process of preparing fresh pineapple juice from rind or core for the production of polyhydroxybutyrate, which consists of the following steps (1.1) fresh pineapple peel and cores are squeezed by a separate juicer. Water and residue until coarse juice (1.2) Take the coarse juice from item (1.1) to adjust the pH value in the range 6.5-7.5 and filter through the No. 1 Waterman filter paper and then coarse juice. It was sterilized at the standard temperature of 121 degrees Celsius under the pressure of 15 psi for 15 minutes and then cultured at 30-37 degrees Celsius. Shaking rate of 100-300 rpm for the production. Polyhydroxybutyrate 2. The process of preparing the coarse juice from the peel or core of fresh pineapple for the production of polyhydroxybutyrate. The Tirate according to claim 1, which also includes additional procedures Bring the coarse juice from item (1.1) to add disodium. Hydrogen phosphate (Na2HPO4) 3-5 g / l Potassium dihydrogen phosphate (KH2PO4) 1-2 g / l Magnesium sulfate Heptahydrate (MSO4-7H2O) 0.1-1.0 g / l Ferric ammonium cite (Fe (lll) (NH4) citrate) 0.01-0.1 g / l, calcium chloride dihydrate (CaCl2-2H2O) 0.01-0.10 g / l and a low trace element solution, volume 1 ml. Contains boric acid (H3BO4) 0.1-0.5 g / l, cobal chloride hexahydrate (CoCl2-6H20) 0.1-0.5 g / l zinc sulfate, heptahydrate (ZnSO4-7H2O). 0.01-0.03 g / l Manganese chloride tetrahydrate (MnCl2-4H2O) 0.01-0.05 g / l Ammonium heptamolybdate tetrahydrate ((NH4) 6Mo7024-4H20) 0.01-0.05 g / l, nickel chloride hexahydrate (NiCl2-6H20) 0.01-0.05 g / l and copper sulfate pentahydrate (CuS04 * 5H20 ) 0.01-0.05 g / l, adjust the carbon / nitrogen ratio in the range 20-200, adjust the pH in the range 6.0-8.0, then filter through the No. 1 Waterman filter paper and then autoclave at standard conditions. The temperature was 121 degrees Celsius under the pressure of 15 psi for 15 minutes and then cultured. Microorganisms at 30-37 ° C, shaking rate of 100-300 rpm for production Polyhydroxybutyrate