TH1671B - Process for purification of hepatitis B virus surface antigen - Google Patents

Claims (7)

1. Procedure for preparation of surface antigen of hepatitis B virus from Lala with surface antigen of hepatitis B virus by column chromatography method improved using ester. Of acid Sulfur with polysaccharides or cellulose with a gel bond. For making chromatography 2. Clause in claim 1, whereby the sinter of sulfuric acid and polysaccharide of the weld line Choose from a group containing cellulose sulfate. Atherosulphate welding wires and extruded sulphate welding wires 3. Clause in claim 2, where cellulose sulfate welding line is cellulose sulfate. Linked by Epichlorohydrin 4. Clause in claim 2, where agarose sulfate is coupled agarose sulfate with epichlorohydrin-linked. 5. The method described in claim 2, where dexanthan sulfate is dextran sulfate, an epichlorohydrin link. 6. Clause in claim 1 whereby the esters ask for acid Sulfur vs cellulose Is obtained from the ester of sulfuric acid with Selected cellulose from cristallin, cellulose, or cellulose containing Cristallin area Or no area of Kristallin 7. Kra. Method of purification of hepatitis B virus surface antigen, which consists of ester gel packaging process. Ask for sulfuric acid with polysaccharide or cellulose, bond into column, balance column with buffer solution with ion intensity 0.001 to 1.0 and pH range 4 to 10 through anhydrous solution. Geni, the surface of the hepatitis B virus onto The column was then washed off the surface antigens of the hepatitis B virus, which were absorbed on the column, using a buffer solution with ion intensity 0.1 to 5 and higher than the ion intensity. In the buffer used, balance the column and the pH used in the range 4 to 10 (7 claims, 1 page, 0 figure).