TH1601003461A - Monoclonal antibodies for the diagnosis of foot stomatitis And how to produce monoclonal antibodies that - Google Patents
Monoclonal antibodies for the diagnosis of foot stomatitis And how to produce monoclonal antibodies thatInfo
- Publication number
- TH1601003461A TH1601003461A TH1601003461A TH1601003461A TH1601003461A TH 1601003461 A TH1601003461 A TH 1601003461A TH 1601003461 A TH1601003461 A TH 1601003461A TH 1601003461 A TH1601003461 A TH 1601003461A TH 1601003461 A TH1601003461 A TH 1601003461A
- Authority
- TH
- Thailand
- Prior art keywords
- foot
- mouth disease
- monoclonal antibodies
- monoclonal antibody
- animals
- Prior art date
Links
- 102000005614 monoclonal antibodies Human genes 0.000 title claims abstract 13
- 108010045030 monoclonal antibodies Proteins 0.000 title claims abstract 13
- 229960000070 antineoplastic Monoclonal antibodies Drugs 0.000 title claims abstract 4
- 229960000060 monoclonal antibodies Drugs 0.000 title claims abstract 4
- 208000003265 Stomatitis Diseases 0.000 title claims 2
- 238000003745 diagnosis Methods 0.000 title claims 2
- 241001465754 Metazoa Species 0.000 claims abstract 6
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 claims abstract 5
- 238000009007 Diagnostic Kit Methods 0.000 claims abstract 4
- 241000710198 Foot-and-mouth disease virus Species 0.000 claims abstract 2
- 102000004965 antibodies Human genes 0.000 claims abstract 2
- 108090001123 antibodies Proteins 0.000 claims abstract 2
- 238000002823 phage display Methods 0.000 claims abstract 2
- 102000004169 proteins and genes Human genes 0.000 claims abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract 2
- 239000000126 substance Substances 0.000 claims abstract 2
- 101800000345 Protein 3ABC Proteins 0.000 claims 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
Abstract
การประดิษฐ์นี้เกี่ยวข้องกับการผลิตโมโนโคลนอลแอนติบอดี (MAb) เพื่อนำไปใช้ในชุดตรวจโรค ที่เกิดจากการแพร่ระบาดของโรคปากและเท้าเปื่อย โดยใช้เทคนิคฟาจดิสเพลย์ (Phage display technology) ในการผลิตโมโนโคลนอลแอนติบอดี (MAb) ที่เฉพาะกับโปรตีน 3ABC ของโรคปากและเท้าเปื่อย ซึ่งเป็นการตัดต่อนำแอนติบอดียีนที่เพิ่มปริมาณด้วยวิธีพีซีอาร์ (PCR) จากสัตว์ที่ติดเชื้อหรือได้รับการฉีดเชื้อที่สนใจ โดยโมโนโคลนอลแอนติบอดีที่ได้จะถูก นำไปใช้เป็นสารหลักในชุดตรวจวินิจฉัยเพื่อแยกสัตว์ที่ติดเชื้อไวรัสโรคปากและเท้าเปื่อยออกจาก สัตว์ที่ได้รับวัคซีน The invention involved the production of a monoclonal antibody (MAb) for use in diagnostic kits. Caused by the spread of foot and mouth disease Phage display technology was used to produce a monoclonal antibody (MAb) specific to the 3ABC protein of foot and mouth disease. This was an amelation of the antibody gene enriched by PCR method from infected or injected animals of interest. The monoclonal antibodies obtained are It is used as the main substance in diagnostic kits to isolate animals infected with the foot-and-mouth disease virus from Vaccinated animals
Claims (1)
Publications (1)
Publication Number | Publication Date |
---|---|
TH1601003461A true TH1601003461A (en) | 2020-06-01 |
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