TH11697A - How to prepare a plasmid that possesses both the ability to exhibit characteristics of a Chinese retrovirus and the ability to be modified? - Google Patents

Abstract

This invention provides a method for preparing plasmids capable of both retroviral gene expression and post-translation enrichment, and plasmids with their expression products. The method involves the insertion of a cDNA fragment containing at least one protease gene from a highly expressive retroviral gene pool or a gene-directed expression vector. This allows for the expression of the retroviral gene and, conversely, the enrichment of its expression product with the expressed product protease, generating high concentrations of the three core proteins encoded by the gag gene and three enzymes encoded by the pol gene, either as standalone or active proteins. Alternatively, the method involves the insertion of a cDNA fragment containing the HIV nef gene region into a highly expressive gene and a gene-directed expression vector that generates the nef protein, a product of the highly expressive nef gene.

Claims (9)

1. A method for preparing plasmids capable of both retroviral gene expression and post-translational enhancement, consisting of the following steps: Bonding by inserting a prepared retroviral gene cDNA fragment containing at least a gene protease region into DNA selected from a group containing a high proportion of expressed genes and vectors whose expression is directed by the gene. 2. A method for preparing the plasmid as claimed in claim 3.1, where the retrovirus is the HIV/AIDS virus. 3. Plasmids possessing both retroviral gene expression and post-translational enhancement capabilities, involving the insertion of a prepared retroviral gene cDNA fragment containing at least a protease region into DNA selected from a pool of highly expressed genes and a gene-directed expression vector. 4. A plasmid as claimed in claim 3, where the retrovirus is the HIV/AIDS virus. 5. Products from plasmid expression possessing both retroviral gene expression and post-translational enhancement capabilities. This involves bonding by inserting a prepared retroviral gene cDNA fragment containing at least one protease region into DNA selected from a pool of highly expressed genes and gene-directed expression vectors. 6. A product exhibiting the characteristics of the plasmid claimed in Claim 5, where the retrovirus is HIV/AIDS. 7. The product of expression of the plasmid claimed in Claim 5 or 6, whereby at least one of the proteins from the group comprising the core protein and the enzyme encoded by the gag and pol genes. 8. Plasmids are constructed by inserting a cDNA fragment of a prepared retroviral gene containing the HIV nef gene region into DNA selected from a pool containing a high concentration of the gene and a vector expressing the gene-directed trait. 9. Products from the expression of bonded plasmids by inserting a prepared retroviral gene cDNA fragment containing the HIV nef gene region into DNA selected from a pool of highly expressed genes and gene-directed vectors (9 claims, 2 pages, 4 figures).