TH10632U - Kit for detecting Gnathostomiasis - Google Patents

Abstract

DC60 (20/08/57) Gnathostomiasis Diagnostic Kit contains: an antigen strip, reagent A (for absorbing antibodies against bacteria), reagent B (for washing the antigen strip), reagent C (enzyme conjugate solution), reagent D (substrate dissolving solution), and reagent E (hydrogen peroxide solution). The kit includes a 30% H2O2 substrate, reagent F (positive control), reagent G (negative control), a reaction tray, test tubes, a color chart, and a few forceps. This gnathostomiasis diagnostic kit is designed to detect specific antibodies in the serum of patients infected with Gnathostoma spinigerum, a type of gnathostomiasis found in the cutaneous, cerebral and cerebrospinal, and visceral regions. The accuracy, sensitivity, predictive positive, and predictive negative percentages are 97.4%, 100%, 96.1%, 92.6%, and 100%, respectively. Results are read visually and reported within 2 hours. (Gnathoustomisais) consists of an antigen strip, reagent A (for adsorbing antibodies against bacteria), reagent B (for rinsing the antigen strip), reagent C (enzyme conjugate solution), reagent D (for dissolving the substrate), reagent E (30% hydrogen peroxide (H2O2) solution), reagent F (positive control), and reagent G (negative control). A number of reaction trays, test tubes, color charts, and forceps are used. The gnathostomiasis diagnostic kit is designed to detect specific antibodies in the serum of patients infected with Gnathostoma spinigerum, a type of gnathostomiasis found in the cutaneous, cerebral and cerebrospinal, and visceral regions. The accuracy, sensitivity, predictive positive, and predictive negative percentages are 97.4%, 100%, 96.1%, 92.6%, and 100%, respectively. Results can be read visually and reported within 2 hours.

Claims (7)

1. The kit for detecting Gnathous worm infection (Gnathous tomisais) contains: - A number of antigen strips, which are membranes containing proteins from genes. Matrix metalloproteinase of the gnathostomiasis parasite (Gnatostoma). (spinigerum) is attached. -Reagent A is the solution used to absorb antibodies against bacteria, and it contains Escherichia coli bacteria. A 1% lysate (Escherichia coli lysate) solution in a pH 7.5 PBS solution (0.1M PBS, pH 7.5) was used. 7.5) Concentration 0.1 molar - Solution B is the solution used to wash the antigen plate, consisting of 0.1% Tween 20 and milk. 1% low-fat skimmed milk powder in a PBS solution, pH 7.5. 0.1 molar concentration -Reagent C is the enzyme conjugate solution, which is the antibody solution of sequence number... 2 (secondary antibodies) labeled with the enzyme horseradish peroxidase; HRP (Hydrogen-Related Polymerase Chain) containing 0.005% enzyme-labeled second antibody and milk fat powder. 1% low-fat skimmed milk in a 0.1 molar (0.1M) PBS solution with a pH of 7.5. (PBS, pH 7.5) - Solution D is the solution used to dissolve the substrate, which is a three-phase solution with a pH of 7.6 and a specific concentration. 50 millimolar (50 mM Tris HCl, pH 7.6) - Solution E is a 30% hydrogen peroxide (H2O2) solution. - The substrate is diaminobenzidine tetrahydrochloride. tetrahydrochloride (DAB) -Reagent F is a positive control, which is the total serum that gives a positive result when detecting antibodies specific to the band. An antigen weighing 32 kilotaltons. -Reagent G is a negative control, which is a mixed serum that gives a negative result when detecting antibodies specific to the band. An antigen weighing 32 kilotaltons. - A number of reaction trays are trays with grooves that are marked to indicate the reaction. Positive tests, negative tests, and tests for samples that need to be examined. (sample test) - A number of test tubes marked to indicate a positive test result. Positive tests (negative tests) and sample tests. - Color chart - A number of forceps.